Determination of Green Soybean (Edamame) Quality using Near-Infrared Spectroscopy (Part + )*
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1 1+ 0 : 32 +*/ **3 + * + - * * * * / 0 - * * * ninhydrine reaction quantity Infratec++ R *13 SECV *+0 R *0/ SECV *0 R *2* SECV *+0 R *02 SECV * Determination of Green Soybean (Edamame) Quality using Near-Infrared Spectroscopy (Part + )* Determination of Eating Quality-Related Constituent Content of Green Soybean (Edamame) Seeds + - Yoshie SUE* Motoyasu NATSUGA* Hiroaki EGASHIRA* Takeshi IKEDA* Tomoya CHIDA* Nanako HORINOUCHI* Tsuneya AKAZAWA* / 0 - Abstract We have been studying the determination of the eating quality-related constituents of green soybean (edamame) seeds such as sucrose and (defined by ninhydrine reaction quantity which has high positive correlation with total free amino acids) using a commercial near-infrared (NIR) transmission spectrometer (Infratec ++ ) We obtained results of R * 13 and SECV * +0 **/ 3 1 **/ **0 3 1 ** /// + - TEL *-/ 2 3*0 Faculty of Agriculture Yamagata University Tsuruoka 3312/// Japan :JA 30* * TEL * // -*+ Present address : ZEN-NOH Fukushima Fukushima 30* *-+ Japan 3312/// + - TEL *-/ 2 3*0 Faculty of Agriculture Yamagata University Tsuruoka 3312/// Japan /// + - TEL *-/ 2 3*0 Faculty of Agriculture Yamagata University Tsuruoka 3312/// Japan : 33* 2/*0 - / TEL *-0 Present address : DENROKU Co Ltd Yamagata 33* 2/*0Japan / : * *** +31* TEL *+312+ /** Present address : AJITECH Co Ltd Kitakami * *** Japan 0 : -++ +/ TEL * Present address : Rokko Region Agriculture and Forestry O$ ce Ibaraki Prefecture Hokota -++ +/+1 Japan Corresponding author : toko@tds + tryamagata-uacjp
2 : + 99 for and R *0/ and SECV *0 for sucrose for room temperature samples Samples cooled to C were added to room temperature samples and calibrations were developed The results of R *2* and SECV *+1 for and R *02 and SECV *- for sucrose for room temperature and cooled samples were su$ cient to determine eating quality-related constituents of edamame seeds in the field [Keywords] near-infrared spectroscopy (NIR) edamame dadachamame quality sucrose ninhydrine reaction quantity () I et al **0 0* wb Natsuga et al **1 Akazawa et al ** Infratec++ * Iwata et al +313 II + ** **- Near-Infrared Spectroscopy : NIR Spe- NIAS ctroscopy +3 Natsuga et al +33 a 0 2 ** +33 b +33- Natsuga et al ** a 2 ** b **- Natsuga et al ** 3 Kawamura et al **1 -* * Velasco Velasco +** g + +/ * et al +311 Perez-Vich Velasco et al +332 Sato Sato et al **- Golebiowski 3 + Golebiowski ** +** * + g Golebiowski et al **/ Arganosa - Arganosa et al **0 Font h Font
3 **3 + Table + Physical properties of green soybean seeds Table Chemical composition of green soybean seeds ** **0 ** **0 n* n ** g +/14/ 240 w4b4 // SD** +141 SD -42 /*043 *41+ + gl 2234/ 421 / SD** 04 SD * *4+0 mm *4+ +43/ +4 *42/ SD** +43 SD *4-/ mm +*4+ - SDD* SD** +4- Table - The repeatability of chemical analysis SDD* 4 mm SD** *42 ** **0 *4 *4++ * *Standard Deviation of the Di# erence : ** SD Yem et al +3// SDD : Standard Deviation of the Di# erence - SDD * SDD Infratec++ Foss The Unscrambler 3 A/S Hillerd Denmark CAMO Oslo Norway Full Cross-validation 2/* +*2 nm nm -* mm +* +** g 3 - /- - + /- Test Set Test Set / g+-/ h +* g / TI- +** Heiko Tokyo Japan JA Sucrose/D-Glucose Test Kit Roche Darmstadt Germany Nishizawa Nishizawa et al +332 Yem
4 : **0 **0 / / Fig + Original spectra and the nd derivative (-D) Fig Original spectra and the nd derivative (-D) ( / ) spectra for room temperature samples of ( / ) spectra for cooled samples of **0 green **0 green soybean seeds soybean seeds III Full cross-validation PLS + + **0 Table Calibrations for determining sucrose content in green soybean using full cross-validation Savitzky- Golay / SECV**** * n R ** nf*** ** **0 R -1 * *40/ ++ *40 Maeda / ** **0 RC --/ * * *4 2/ O-H +0+ nm ++2 nm Maeda *RC ** *** PLS et al +33/ Kawano **** Cross-validation Standard error of cross-valida- 2+ nm 300 nm tion Kawano and Abe +33/ + 30* nm O-H Full Cross-validation +
5 **3 / Full cross-validation PLS Table / Calibrations for determining in green soybean using full cross-validation n SECV R nf ** **0 R ** **0 RC -1 --/ *4-* +43/ *4-* 4*- *413 *42* *4+0 *4+0 Full Cross-validation - / Full Cross-validation - ** **0 Full Cross- Validation + / Fig - Scatter plot for full cross-validation using ** **0 **-**0 all samples (sucrose) R *13 SECV *+0 ** **0 **0 ** **0 R * 03 SEP * Bias * SEP R *0/ SECV *0 Natsuga et al +33 Williams and Norris **+ ** **0 **0 R * *1 SEP ** **0 *1- SEP Full Cross-validation R *2* SECV *+0 Test Set R *2+ SECV * *-+ *- ** **0 / Full Cross-validation R *02 SECV * Test Set R *02 SEP */ ++ * Full Cross-validation ** **0 -
6 : ** **0 Full Cross- Validation Fig Scatter plot for full cross-validation using ** **0 all samples () 0 Fig 0 Scatter plot for NIR determination of sucrose content in green soybean in a practical application / Fig / Capture of quality estimation program for dadachamame ** **/ Full Cross-validation R *0 SECV *2 R * 1 SECV * +1 **/ **0 JA 0 1 R *2/ SEP *2 R *21 SEP *+ IV Microsoft Visual Basic 0* / Infratec++ PC : Infratec++ Akazawa et al ** Infratec++
7 **3 NIAS Infratec ++ References 1 Fig 1 Scatter plot for NIR determination of in green soybean in a practical application + R *13 SECV *+0 R *0/ SECV *0 R *2* SECV *+1 R *02 SECV * - **/ **0 JA R *21 SEP *+ R *2/ SEP *2 +/ * Akazawa T Takahashi H ** Varietal di# erences in Concentrations of Sugar and Water-soluble Nitrogen in Vegetable-type and Grain-type Soybean Cultivars Bulletin of the Research Farm Faculty of Agriculture Yamagata University + 3 /0 Arganosa G C Darkentin T Racz V J Blade S Phillips C Hsu H **0 Prediction of crude protein content in field peas using near infrared reflectance Canadian Journal of Plant Science 20 ( + ) +/1 +/3 Font R Del Rio-Celestino M De Haro-Bailon A **0 The use of near-infrared spectroscopy (nirs) in the study of seed quality components in plant breeding programs Industrial Crops and Products (-) -*1-+- Golebiowski T ** Near infrared reflectance spectroscopy of oil in intact canola seed (Brassica napus L) I Journal of Near Infrared Spectroscopy + (/) -/ --* Golebiowski T Leong AS Panozzo JF **/ Near infrared reflectance spectroscopy of oil in intact canola seed (Brassica Napus L) II Journal of Near Infrared Spectroscopy +- () // 0 Iwata T Shirahata K +313 Keeping quality of green soybeans I The factors concerning post harvest deterioration and the e# ect of atmosphere and whole plant packaging(in Japanese) Journal of Japanese Society for Horticultural Science 2 ( + ) +*0 ++- Kawamura S Kawasaki M Nakatsuji H Natsuga M **1 Near-infrared spectroscopic sensing system for online monitoring of milk quality during milking Sensing and Instrumentation for Food Quality and Safety Kawano S Abe H +33/ Development of a calibration equation with temperature compensation for determining the Brix value in intact peaches Journal of Near Infrared Spectroscopy - () Maeda H Ozaki Y Tanaka M Hayashi N Kojima T +33/ Near infrared spectroscopy and chemometrics studies of temperature-dependent spectral variations of water : relationship between spectral changes and hydrogen bonds Journal of Near Infrared Spectroscopy - () +3+ *+ Natsuga M Kawamura S Itoh K +33 Precision and accuconstituent content of grain (Part ) (in Japanese) racy of near-infrared reflectance spectroscopy in determining Journal of JSAM / ( 0) 233- Natsuga M Kawamura S Itoh K +33- Accuracy of nearinfrared reflectance spectroscopy in determining constituent content of grain(part -) (in Japanese) Journal of JSAM // ( + ) +*3 ++/ Natsuga M Kawamura S Itoh K +33 Factors a# ecting in determining constituent content of grain by nir spectroscopy (Part ) (in Japanese) Journal of JSAM /0(-) --/*
8 : Natsuga M Kawamura S Itoh K ** E# ects of wave- of green soybeans from single pod measurements Jourlength range and light path-length on the accuracy of nal of Near Infrared Spectroscopy +/ -1-- the constituent analysis of unhomogenized milk using Perez-Vich B Velasco L Fernandez-Martinez JM +332 near-infrared spectroscopy (in Japanese) Journal of Determination of seed oil content and fatty acid compo- JSAM 0 (/) 2-22 sition in sunflower through the analysis of intact seeds Natsuga M Kawamura S Itoh K +33 Accuracy of near- husked seeds meal and oil by near-infrared reflectance infrared reflectance spectroscopy in determining constit- spectroscopy Journal of the American Oil Chemists Souent content of grain (Part + ) (in Japanese) Journal of ciety 1/ (/) /1 /// JSAM / ( + ) 233- Sato T Maw A Katsuta M **- NIR reflectance spectro- Natsuga M Nakamura A Kawano S ** A study on scopic analysis of the FA composition in sesame ( sesamum high-speed sorting of single-kernel brown rice using indicum L) seeds Journal of the American Oil Chemists near-infrared spectroscopy (Part + ) (in Japanese) Journal Society 2* ( + ) ++/ of JSAM 0 ( + ) +** +*/ Velasco L De Haro A Fernandez-Martinez JM +331 Deter- Natsuga M Nakamura A Kawano S ** A study on mination of the fatty acid composition of the oil in high-speed sorting of single-kernel brown rice using intact-seed mustard by near-infrared reflectance specnear-infrared spectroscopy (Part) (in Japanese) Journal troscopy Journal of the American Oil Chemists Society of JSAM 0 ( + ) +* ( + ) +/3/ +0* Natsuga M Nakamura A Kawano S **- A study on Williams P Norris K **+ Near-Infrared Technology in the high-speed sorting of single-kernel brown rice using Agricultural and Food Industries (Second Edition) near-infrared spectroscopy (Part-) (in Japanese) Journal American Association of Cereal Chemists Inc Minnesoof JSAM 0/ (-) +*1 ++- ta USA Natsuga M Sue Y Ikeda T Egashira H Akazawa T Ura N Yemm E W Cooking E +3// The determination of amino **1 Development and evaluation of dedicated bench- acids with ninhydrine Analyst 2* *3 +- type near infrared spectrometers to estimate the flavour : **2 2 3 : *+* + -+
Determination of Green Soybean (Edamame) Quality Using Near-Infrared Spectroscopy (Part,)*
1- + : /+ /0 *++ * + 1 * * * * / 0 - * * * :NRQ + Infratec++ +/ * R */1 SECV *3 NRQ R *03 SECV *+ +/ NRQ Determination of Green Soybean (Edamame) Quality Using Near-Infrared Spectroscopy (Part )* Constituent
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