SUPPLEMENTARY FIGURES
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1 SUPPLEMENTARY FIGURES Supplementary Figure 1. Gel permeation chromatograms of a cleavable diblock copolymer before and after UV irradiation. Multi-peak-fitting analysis was performed to estimate the percentage of cleavage. After very short irradiation times ( s), the peak of the larger PEG-b-PLA (in red) starts to decrease while the peak of the shorter PEG (in blue) starts to increase. Photocleavage appears to reach a plateau after ~60 s of irradiation. 1
2 Supplementary Figure 2. A. 60-nm nanoparticles with different content of cleavable diblock copolymer can be synthesized. After synthesis, all nanoparticles have the same overall PEG density (full triangles). Upon UV irradiation, the PEG density on the surface of the nanoparticles (empty triangles) is inversely proportional to the amount of cleavable diblock copolymer used in the preparation of the nanoparticles. B. Shedding of the PEG corona does not appear to significantly affect the size distribution of the nanoparticles, possibly because the negative surface charges (COO ) cause electrostatic repulsion. C. The shedding of the PEG corona increases the negative charge of the nanoparticles. D. Neutralization of the surface charge by cations (Ca 2+ or Na + ) or acidic ph (ph 1) triggers precipitation of the nanoparticles. 2
3 [ 14 C]-NP remaining in solution (%) Without CaCl 2 With 5 mm CaCl UV irradiation time (s) Supplementary Figure 3. After very short UV irradiation, the nanoparticles lose their protective PEG layer and precipitate in presence of calcium. The time needed to precipitate the nanoparticles is in agreement with the kinetics of copolymer cleavage obtained from GPC data. 3
4 Supplementary Figure 4. The adsorption of dyes onto nanoparticles is evidenced by phase separation. In order to avoid photobleaching of the dyes, the nanoparticles were irradiated prior to incubation with the dyes. 4
5 Supplementary Figure 5. A The partitioning into nanoparticles of chemicals with different physicochemical properties appears to increase with decreasing particle size. Values represent each replicate, n = B. The increase in partitioning appears to correlate with an increase in surface-to-volume ratio. To facilitate comparison, partition coefficients for each chemicals were normalized to those obtained with 160-nm nanoparticles. Values represent mean ± standard deviation, n =
6 Supplementary Figure 6. For all tested particle sizes, the adsorption onto nanoparticles appears to correlate with the hydrophobicity of the small molecules, suggesting that hydrophobic interactions are at least partly responsible for the interactions between the chemicals and the nanoparticles. 6
7 Supplementary Figure 7. In the absence of nanoparticles (dark red), the photodegradation of most chemicals is reduced, suggesting that the photocleavable linker plays an active role in the reaction. Different degradation mechanisms might be involved since, for some chemicals (i.e., pentachlorophenol, triclosan and methoxychlor), photodegradation appears to be reduced in the presence of nanoparticles (light pink). 7
8 Supplementary Figure 8. A. To show that the reduced teratogenicity observed after treatment with photo-responsive nanoparticles was due to extraction and photodegradation of BPA, control experiments were carried out in which an equivalent amount of BPA was irradiated with UV light (in gray), or irradiated with UV light in the presence of non-photo-responsive nanoparticles (in dark blue). In both groups, all fish died during the 2 nd day post exposure to BPA, while fish not exposed to BPA survived (> 90% survival) (n = 96, p < 0.001). B. Functionalization of PEG with the hydroxyethyl photolinker did not enhance the teratogenicity of the polymer either before or after UV irradiation (> 90% survival). Here, the concentrations of the functionalized polymers were 10 times higher than those of unmodified PEG (log rank, p = 0.788, n = 48). 8
9 a. b. Supplementary Figure 9. A. Amount of BPA extracted from thermal printing paper using acetonitrile. The total BPA content was calculated by adding the values of two consecutive extraction steps. B. The amount of BPA remaining in the thermal printing paper is inversely proportional to the amount extracted with water or nanoparticles. 9
10 SUPPLEMENTARY TABLES Supplementary Table 1: Molecular weight and polydispersity of photocleavable diblock copolymers Copolymer Mn (NMR) a Mn (GPC) b Mw (GPC) b PDI c PEG5k-b-PLA5k ,679 18, PEG5k-b-PLA10k 14,648 17,888 20, PEG5k-b-PLA20k 25,184 21,658 25, PEG5k-b-PLA35k 39,380 30,264 42, a Relative molecular mass determined by 1 H-NMR (CDCl3, 300 MHz) b Relative molecular mass determined by GPC against polystyrene standards c Polydispersity index (Mw / Mn) 10
11 Supplementary Table 2: Copolymer concentrations used for the different formulations (in mg ml -1, total polymer concentration 10 mg ml -1 ) and representative sizes of the prepared nanoparticles Fig. 4 Fig. 3 Fig. 2 Fig. 1D Suppl. Fig. 1 Fig. 1C PEG5k-linker-PLA35k PEG5k-linker-PLA20k PEG5k-linker-PLA10k PEG5k-linker-PLA5k PEG5k-PLA20k PLGA30k PLGA95k Radioactive PLGA20k Size (mean ± SD) 20% ± ± % ± ± % ± ± % ± ± % ± ± % ± ± % ± ± % ± ± % ± ± nm ± ± nm ± ± nm ± ± nm ± ± nm ± ± nm ± ± nm ± ± nm ± ± nm ± ± nm ± ± Panel A ± ± Panel B ± ± Water ± ± Thermal paper ± ± Soil ± ± PDI 11
12 Supplementary Table 3: HPLC methods for all compounds Molecule Injection volume (µl) Flow (ml min -1 ) Acetonitrile (%) Water 0.1% TFA (%) Ret. Time (min) Detector λ (nm) Bisphenol A FLD 225 / α-Ethinyl estradiol FLD 225 / β-Estradiol FLD 225 / 310 Bisphenol S MWD 254 Carbamazepine MWD 285 Chlordane MWD 210 DEHP MWD 210 4,4'-DDT MWD 210 Benzo[a]pyrene FLD 230 / 460 2,4-Dichlorophenol MWD 227 Docetaxel MWD 227 Fluoxetine FLD 230 / 290 Furosemide MWD 230 Gemfibrozil FLD 242 / 300 Picloram MWD 230 Probenecid MWD 250 Propranolol FLD 230 / 340 Rotenone MWD 210 Triclosan MWD 210 Methoxychlor MWD 210 Pentachlorophenol MWD 210 Dinoseb MWD 280 Biphenyl MWD
13 SUPPLEMENTARY METHODS Photocleavage of PEG-b-PLA copolymers To investigate the photocleavage of PEG-b-PLA copolymers, 20 mg of PEG5k-b-PLA10k were dissolved in 2 ml of acetonitrile. The solution was split into two batches; in each case, 1 ml of this solution was dropped into 10 ml of water and stirred over night under light protection to evaporate the acetonitrile. All batches were combined and concentrated to 2 ml by using Amicon Ultra-15 Centrifugal Filter Units, molecular weight cut-off 100 kda (EMD Millipore, Billerica, MA). Aliquots of 250 µl were irradiated with UV light ( nm, DYMAX BlueWave 200 UV Curing Spot Lamp, Dymax Corporation, Torrington, CT) for 0 s, 5 s, 10 s, 30 s, 60 s, and 300 s; the light intensity was adjusted to 10 mw cm -2. The irradiated samples were freeze-dried and dissolved in 500 µl of chloroform. The samples were analyzed by gel permeation chromatography (GPC) on a Shimadzu 10AVP HPLC system equipped with a Shimadzu RID-10A refractive index detector (Shimadzu Deutschland GmbH, Duisburg, Germany) at 40 C. A Phenogel 5 µm 500 Å column was used in combination with a Phenogel 5 µm guard column (Phenomenex, Aschaffenburg, Germany). One hundred µl of each sample were injected; chloroform was used as mobile phase (flow rate 1 ml min -1 ). Precipitation of nanoparticles in the presence of NaCl or acidic ph To evaluate the effect of NaCl or different ph values on the precipitation of nanoparticles, 10 µl of [ 14 C]-labeled nanoparticles were added to 80 µl of water, 10 mm acetic acid buffer (ph 4) or 10 mm trifluoroacetic acid buffer (ph 1). The samples were incubated for 10 min, and then supplemented with 10 µl of water, 50 mm CaCl2 or 1.5 M NaCl. Afterwards, the samples were irradiated with UV light for 1 min ( nm, 10 mw cm -2, DYMAX BlueWave 200 UV Curing Spot Lamp, Dymax Corporation, Torrington, CT). The irradiated samples were then centrifuged for 5 min at 100 rcf; then, 20 µl of the supernatant were analyzed by scintillation counting on a Tri-Carb 2810 TR Liquid Scintillation Analyzer (Perkin Elmer, Waltham, MA). The amount of particles remaining in suspension was measured by comparing the measured radioactivity to that in untreated samples. Each experiment was conducted in triplicate. High performance liquid chromatography (HPLC) quantification The concentration of chemicals in solution was quantified using reverse-phase HPLC on a 1260 Infinity system (Agilent, Santa Clara, CA) equipped with a quaternary pump (1260 Quat Pump VL GB11C), an autosampler (1260 ALS G1329B), a thermostatted column compartment (1260 TCC G1316A), a multiple wavelength detector (1260 MWD VL G13265D) and a fluorescence detector (1260 FLD G1321B). A C-18 Zorbax 300SB-C18 column, 5 µm, 4.6 x 250 mm was used. The chemicals were separated by isocratic elution using different mixtures of HPLC-grade acetonitrile (Sigma-Aldrich, St-Louis, MO) and 0.1% TFA in ultrapure water (Millipore, Billerica, MA). The mobile phase compositions as well as the detection wavelengths are presented in Supplementary Table 3. 13
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