Belgrade Food International Conference. Food, health and well being. Belgrade, 26 th to 28 th November 2012.

Transcription

1 Belgrade Food International Conference Food, health and well being Belgrade, 26 th to 28 th November 2012.

2 Belgrade Food International Conference Prof. Dr. Marija Gavrović-Jankulović, President of the Belgrade Food International Conference Scientific Committee: Prof. Dr. Tanja Ćirković Veličković (President) Dr. Estelle Bonnin Dr. Ivan Minkov Dr. Guro Gafvelin Dr. Theodore Sotiroudis Dr. Arnd Petersen Dr. Joost Smit Dr. Marija Glibetić Prof. Dr. Zoran Vujčić Prof. Dr. Živoslav Tešić Prof. Dr. Miroslav Vrvić Organizing Committee: Dr. Dragana Stanić-Vučinić (President) Dr. Nenad Milosavić Milica Grozdanović Jana Ognjenović Marija Stojadinović Jelena Radosavljević Jasna Nikolić 1

3 Content: 1. Session 1: Enzymes in food processing 2. Session 2: Wastes and biomass valorization 3. Session 3: Supplements, micronutrients and food additives 4. Session 4: Food antioxidants 5. Session 5: Nutrition science and bioactive compounds 6. Session 6: New approaches to food analysis 7. Session 7: Food allergens 8. Session 8: Nutrition and immunology 9. Session 9: Molecular biotechnology for the benefit of consumers 10. Session 10: New functional foods 11. Session 11: Health effects of food 12. List of poster presentations 2

4 P Antioxidative properties of infant formulas against hydroxyl radical production in the Fenton reaction N. Lugonja 1, O.B. Laugier 1, M.M.Vrvić 1,2, S.Miletić 1, S. D. Spasić 1 1 Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Belgrade, Njegoševa 12, P.O.Box 473, Serbia 2 Faculty of Chemistry, University of Belgrade, Belgrade, Studentski trg 12-16, P.O.Box 51, Serbia Electron paramagnetic resonance (EPR) spin-trapping spectroscopy was used to compare the antioxidative capacities of commercial infant formulas against hydroxyl radical production in the Fenton reaction. Infant formula used in our experiments was Impamil- Serbia: Impamil 1, Impamil 2, Impamil 3, Impamil Pre, Impamil FL, and Danone- Holland: Bebelac and Milupa-Germany: Aptamil marked as samples 1-7. All formulas were prepared by dissolving 15 g of dry powder in 90 ml water (150 g/l). EPR spectra were recorded using a Varian E104-A EPR spectrometer operating at the X-band (9.572 GHz) with the following settings: modulation amplitude: 0.2 mt; modulation frequency: 100 khz; microwave power: 20 mw; time constant: 32 ms; scanning time: 4 min. The temperature in the cavity was controlled at 293 O K. Spin-trap BMPO (5-tertbutoxycarbonyl-5-methyl-1-pyrroline-N-oxide) was purchased from Enzo Life Sciences International (Plymouth Meeting, PA, USA). Recordings were performed using EW software (Scientific Software, Bloomington, IL, US). The Fenton reaction was performed by adding Fe 2+ (FeSO 4 Sigma-Aldrich) and H 2 O 2 (Reanal, Budapest, Hungary) at final concentrations of 0.6 mm and 3 mm, respectively, followed after the addition of BMPO (10 mm). The samples were drawn into 10 cm long gas-permeable Teflon tubes (wall thickness: mm; internal diameter: 0.6 mm; Zeus industries, Raritan, NJ, USA) in order to maintain constant po 2 levels. Measurements were performed using quartz capillaries in which the Teflon tubes were placed. Recordings were conducted 2 min after the start of the reaction. Spectral simulations of each spectrum were performed using the WINEPR SimFonia computer program (Bruker Analytische Messtechnik GmbH, Darmstadt, Germany). In all samples the generation of hydroxyl radical led to the formation of carbon-centred and ascorbyl radicals. In samples 1-5, the level of carboncentred radical was significantly higher than in samples 6 and 7 but those samples have higher signal of ascorbyl radical in comparison with other samples. In addition to hydroxyl radical scavenging, Fenton reaction may be hampered by the removal of redox active (inappropriately chelated) iron. That fact limit amount of ascorbate which may be added in formulas and other possible mechanisms for increased antioxidative capacity are discussed. 87

5 Antioxidative properties of infant formulas against hydroxyl radical production in the Fenton reaction N. Lugonja1, O.B. Laugier1, M.M.Vrvić1,2, S.Miletić1, S. D. Spasić1 1Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoševa 12, Belgrade, Serbia 2Faculty of Chemistry, University of Belgrade, Studentski trg 16, Belgrade, Serbia. Electron paramagnetic resonance (EPR) spin-trapping spectroscopy was used to compare the antioxidative capacities of commercial infant formulas against hydroxyl radical production in the Fenton reaction. Infant formula used in our experiments was Impamil-Serbia: Impamil 1, Impamil 2, Impamil 3, Ipamil PRE, Impamil FL, and Danone-Holland: Bebelac and Milupa-Germany: Aptamil marked as samples 1-7 (Table 1). All formulas were prepared by dissolving 15 g of dry powder in 90 ml water (36 g/l). EPR spectra were recorded using a Varian E104-A EPR spectrometer operating at the X-band (9.572 GHz) with the following settings: modulation amplitude: 0.2 mt; modulation frequency: 100 khz; microwave power: 20 mw; time constant: 32 ms; scanning time: 4 min. The temperature in the cavity was controlled at 293oK. Spin-trap BMPO (5-tert-butoxycarbonyl-5-methyl-1-pyrroline-Noxide) was purchased from Enzo Life Sciences International (Plymouth Meeting, PA, USA). Recordings were performed using EW software (Scientific Software, Bloomington, IL, US). The Fenton reaction was performed by adding Fe2+ (FeSO4 Sigma-Aldrich) and H2O2 (Renal, Budapest, Hungary) at final concentrations of 0.6 mm and 3 mm, respectively, followed after the addition of BMPO (10 mm). The samples were drawn into 10 cm long gas-permeable Teflon tubes (wall thickness: mm; internal diameter: 0.6 mm; Zeus industries, Raritan, NJ, USA) in order to maintain constant po2 levels. Measurements were performed using quartz capillaries in which the Teflon tubes were placed. Recordings were conducted 2 min after the start of the reaction. Spectral simulations of each spectrum were performed using the WINEPR SimFonia computer program (Bruker Analytische Messtechnik GmbH, Darmstadt, Germany). In all samples the generation of hydroxyl radical led to the formation of carboncentred and ascorbyl radicals. In samples 1-5, the level of carboncentred radical was significantly higher than in samples 6 and 7 but those samples have higher signal of ascorbyl radical in comparation with other samples. In addition to hydroxyl radical scavenging, Fenton reaction may be hampered by the removal of redox active (inappropriately chelated) iron. That fact limit amount of ascorbate which may be added in Formulas and other possible mechanisms for increased antioxidative capacity are discussed (Figure 1). Table 1. Figure 1. References: 1. Lugonja N, Spasić SD, Laugier O, Nikolić-Kokić A., Spasojević I., Oreščanin-Dušić Z., Vrvić M.M. Differences in direct pharmacological effects and antioxidative properties of mature breast milk and infant formulas. Nutrition 2012; doi.org/ /j.nut Friel JK, Martin SM, Langdon M, Herzberg GR, Buettner GR. Milk from mothers of both premature and full-term infants provides better antioxidant protection than does infant formula. Pediatr Res 2002; 51(5): Acknowledgements: This work was supported by the Ministry of Education Science of the Republic of Serbia, Grants # *Corresponding author: Snežana D. Spasić, PhD, Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoševa 12, Belgrade, Serbia; tel: ; svujin@chem.bg.ac.rs