Supporting Information
|
|
- Arleen Hicks
- 6 years ago
- Views:
Transcription
1 Supporting Information Enzymatic Reactor with Trypsin Immobilized on Graphene Oxide Modified Polymer Microspheres to Achieve Automated Proteome Quantification Huiming Yuan #, Shen Zhang #, Baofeng Zhao, Yejing Weng, Xudong Zhu, Senwu Li, Lihua Zhang*,Yukui Zhang CAS Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian , China; University of Chinese Academy of Sciences, Beijing , China. # These authors contributed equally to this work * To whom correspondence should be addressed. Prof. Lihua Zhang: phone, ; fax, ; , lihuazhang@dicp.ac.cn. Table of Contents Materials and Reagents...S-3 Protein extraction...s-3 Preparation of GO@PEI@polymer particles...s-4 Characterization of GO@PEI@polymer particles...s-4 Trypsin immobilization...s-4 Evaluation of the peptide carryover on the IMER...S-5 Trypsin digestion and 18 O labeling...s-5 Life time of the IMER......S-6 MALDI-TOF MS detection... S-6 Data analysis...s-6 References...S-7 Table S-1...S-8 Table S-2...S-8 S-1
2 Table S-3...S-9 Figure S-1... S-10 Figure S-2...S-11 Figure S-3...S-11 Figure S-4...S-13 Figure S-5...S-14 Figure S-6...S-15 Figure S-7...S-16 Figure S-8...S-17 S-2
3 Materials and Reagents Bovine serum albumin (BSA, bovine serum), Trypsin (bovine pancreas, TPCK treated), formic acid (FA), α-cyano-4-hydroxycinnamic acid (CHCA), polyethylenimine (average Mw=800, PEI), and protease inhibitor cocktail were ordered from Sigma Aldrich (St. Louis, MO, USA). Dithiothreitol (DTT) and iodoacetamide (IAA) were obtained from Acros (Morris Plains, NJ, USA). Acetonitrile (ACN, HPLC grade) was purchased from Merck (Darmstadt, Germany). Water was purified by a Milli-Q system (Millipore, Milford, MA, USA). 18 O-Water (97%) was obtained from the Shanghai Research Institute of Chemical Industry (Shanghai, China). All other chemicals and solvents were of analytical grade. A precise syringe pump (Baoding Longer Pump Company, Baoding, China) was used to pump the protein solution through the IMERs. A BCA protein assay kit was obtained from the Beyotime Institute of Biotechnology (Tianjin, China). Acrylic polymer particles with amine groups (10 μm, 1,000 Å) were obtained from Suzhou Nanomicro Technology Inc. (Suzhou, China). Fused-silica capillaries (250 µm i.d. 375 µm o.d.) were obtained from Sino Sumtech (Handan, China). Protein extraction E. coli cells were cultured in LB medium at 37 C for 24 h. The cells were harvested and then washed with ice-cold PBS 3 times, finally suspended in an extraction buffer composed of 8 M urea and 1% (v/v) protease inhibitor cocktail. The suspension was ultrasonicated for 100 s at 100 W and centrifuged at 20,000 g for 30 min. Then, the supernatant was collected as the soluble fraction of E. coli cell lysate. Hepatocarcinoma ascites syngeneic cells with high (Hca-F) and low (Hca-P) lymph node metastasis rates were donated by Professor Shujuan Shao (Dalian Medical University). The grown cells were suspended in an extraction buffer composed of 8 M urea and 1% (v/v) protease inhibitor cocktail, followed by ultrasonication for 100 s at 100 W and centrifugation at 25,000 g for 40 min. The supernatant was collected as the soluble fraction proteins. The supernatants from E.coil, Hca-F, and Hca-P cells were precipitated by the addition S-3
4 of ice-cold acetone. After centrifugation, the pellets were lyophilized by a Speed Vac Concentrator (Thermo Fisher Scientific, CA) and re-dissolved in 50 mm NH 4 HCO 3 (ph 7.8). For 18 O labeling, the NH 4 HCO 3 buffer was made with 18 O-water instead of 16 O-water. The protein concentration was determined by a BCA assay. Preparation of GO@PEI@polymer particles Acrylic polymer particles with amino groups (150 mg, 10 μ, 1,000 Å) were suspended in phosphate buffer (PB, ph 8.0) containing 5% glutaraldehyde (v/v) and vortexed for 3 h at room temperature. After centrifugation, the modified particles were obtained, and the unreacted glutaraldhyde was removed by flushing the particles with ethyl alcohol and water. Then, 5 mg/ml PEI was added to the suspension of the modified particles and reacted for 3 h at room temperature. After centrifugation, PEI-modified polymer particles were obtained, and the unreacted PEI was removed by washing the PEI-modified particles with ethyl alcohol and water. Finally, approximately 75 mg of PEI-modified particles was mixed with 1.5 ml (1 mg/ml) of GO suspension and vortexed for 6 h at room temperature. After centrifugation, the obtained GO@PEI@polymer particles were washed with water, ethyl alcohol, and phosphate buffer. Characterization of GO@PEI@polymer particles Dynamic light scattering (DLS) and zeta potential measurements were performed on a Malvern Nano Z Zetasizer (Worcestershire, UK). The particles were dispersed in water during the measurements. The chemical composition was performed on an X-ray photoelectron spectrometer (XPS, Thermo ESCALAB 250 Xi) with Al Kα radiation as the X-ray source (Thermo, Waltham, MA, USA). Transmission electron microscopy (TEM) analysis was performed on a JEM-2000EX instrument (120 kv, Tokyo, Japan). Trypsin immobilization The GO@PEI@polymer particles were packed into a 250 μm capillary tube; then, a 2 mg/ml trypsin solution containing 50 mm benzamidine was pumped into the capillary tube S-4
5 for 24 h at 4 C. The obtained IMER was flushed by 50 mm ammonium bicarbonate (ph 8.0) to remove the residual trypsin; the unreacted residual trypsin was collected and analyzed by a BCA assay. The IMER was stored at 4 C for further use. For comparison, amino-functionalized polymer microparticles and PEI-modified polymer microparticles were packed into capillary tubes with the same dimensions. The supports were activated by flushing a solution of 5% (v/v) glutaraldehyde for 6 h at room temperature, and trypsin was covalently bonded by continuously pumping 3 mg/ml trypsin dissolved in 100 mm phosphate buffer (ph 8.0) containing 50 mm benzamidine and 5 mg/ml sodium cyanoborohydride for 24 h at 4 C. After being purged with 1 M Tris-HCl (ph 8.0) and 20% acetonitrile (ACN) for 4 h, the IMERs were filled with 0.02% (w/v) NaN3 solution and stored at 4 C before use. Evaluation of the peptide carryover on the IMER The adsorption of peptides on the IMER based on GO@PEI@polymer particles was evaluated by the following steps. The IMER was flushed in sequence with light and heavy formaldehyde-labeled peptides from E. coli (0.1 mg/ml) at a flow rate of 1 μl/min for 10 min. The light fraction was discarded, whereas the heavy fraction was collected and subjected to nano-lc-ms/ms analysis. Trypsin digestion and 18 O labeling For traditional in-solution protocols, BSA and E. coli extracts were digested by trypsin with a substrate-to-enzyme ratio of 25:1(m/m), which was followed by incubation at 37 C for 12 h. After being desalted, the tryptic digests from BSA and E. coli extracts were lyophilized by a Speed Vac Concentrator and re-suspended in trypsin (25:1, m/m) prepared with 100 mm ammonium acetate buffer (dissolved in 18 O-water, ph 6.0). After incubation for 24 h at 37 C, the protein solutions were boiled for 10 min at 100 C to deactivate residual trypsin [1]. For the IMER, BSA and E. coli extracts dissolved in NH 4 HCO 3 buffer (prepared with 18 O-water) were pumped into the IMERs at a flow rate of 1 μl/min at 37 C, and the digests were collected for further MS analysis. Furthermore, in our experiments, we added 16 O-water to redissolve 18 O labeled peptides obtained by the IMER, and kept them at 4 C for a week. The peptides were analyzed by MALDI-TOF MS. S-5
6 Lifetime of the IMER The lifetime of the IMER was evaluated by digestion and 18 O labeling of 0.1 mg/ml BSA at the flow rate of 1 μl/min in consecutive 10 days (100 runs). The collected digests were analyzed by MALDI-TOF MS. MALDI-TOF MS detection MALDI-TOF MS experiments were performed on a Bruker ultraflex III MALDI-TOF/TOF mass spectrometer (Bruker Daltonics, Bremen, Germany) using a Smartbeam laser as an ionization source. With a polished target, 7 mg/ml CHCA dissolved in a 2:1 mixture of ACN/H 2 O containing 0.1% (v/v) TFA was used as the matrix. MALDI-TOF MS experiments were performed with constant laser intensity in the positive ion mode. All mass spectra were obtained with an accumulation of 800 laser shots in the reflector mode with an accelerating voltage of kv. Data analysis For MALDI-TOF MS, several peptides within a broad range of m/z were chosen to calculate the 18 O labeling efficiency from the MALDI data in BSA digests, and the efficiency of incorporation of one or two 18 O atoms was manually calculated according to the following equations [2]: (1) The theoretical intensities of Mi were calculated from its sequence according to the MS-isotope value obtained from the ProteinProspector website ( (2) For LC-MS analysis, the acquired raw files were analyzed with MaxQuant (version S-6
7 ). Cysteine carbamidomethylation was searched as a fixed modification, whereas N-terminal acetylation and methionine oxidation were searched as variable modifications. Enzyme specificity was set as trypsin. Two missed cleavages were allowed, and at least six amino acids were required per identified peptide. For 18 O based quantitative analysis, 18 O was set as the heavy label. References (1) Yang, S. J.; Nie, A. Y.; Zhang, L.; Yan, G. Q.; Yao, J.; Xie, L. Q.; Lu, H. J.; Yang, P. Y. Journal of Proteomics, 2012, 75, (2) López-Ferrer, D.; Ramos-Fernandez, A.; Martinez-Bartolome, S.; Garcia-Ruiz, P.; Vázquez, J. Proteomics, 2006, 6, 4-6. S-7
8 Table S-1. Zeta potentials of GO, polymer particles, particles and particles determined by dynamic light scattering. Zeta potential GO Polymer particle particle ymer particle (mv) / Average Table S-2. Elemental analysis of polymer particles and PEI@polymer particles by X-ray photoelectron spectroscopy (XPS). Atomic PEI@polymer Polymer particle % particle C1s N1s O1s S-8
9 Table S-3. Comparison of the labeling efficiencies obtained by the IMER and a traditional off-line protocol. In the experiments, the 8 most intense peaks from the MALDI-TOF MS spectra were selected. Sequence m/z Labeling efficiency (%) IMER (2.5 min) Labeling efficiency (%) off-line protocol (38 h) K.YLYEIAR.R K.CCTESLVNR R R.FKDLGEEH FK.G K.LGEYGFQN ALIVR.Y K.LKPDPNTL CDEFK.A R.KVPQVSTPT LVEVSR.S K.DAFLGSFLY EYSR.R R.RPCFSALTP DETYVPK.A Average labeling efficiency (%) S-9
10 Figure S-1. TEM morphologies of a) polymer microparticle b) GO, c) hybrid particles ( 4,000), and d) GO@PEI@polymer particles ( 15,000). S-10
11 Figure S-2. MALDI-TOF MS spectra of BSA digests by the IMER in H 2 18 O (a) and H 2 16 O (b). S-11
12 (1) (2) (3) (4) (1) (2) (3) (4) Figure S-3. Comparison of peptide labeling efficiencies obtained by the IMER based on particles (2), particles (3), and polymer particles (4). In the experiments, the same protein digestion was also performed in H 16 2 O (1).The sequences of selected peptides were as follows: a) K.YLYEIAR.R; b) K.VLTSSARQR.L; c) K.LGEYGFQNALIVR.Y; d) R.KVPQVSTPTLVEVSR.S; e) K.DAFLGSFLYEYSR.R; and f) R.RPCFSALTPDETYVPK.A. S-12
13 Figure S4. Evaluation of protein digestion by upimer in proteome quantification. a). The log2 (H/L) ratio distribution of peptides from E. coli samples with and without missed cleavage sites. b). The observed log2 (H/L) ratios of the quantified proteins in triplicate runs. S-13
14 Figure S-5. Carryover of peptides on the IMER. Three representative peptides are listed, and the sequences of these peptides were as follows: a) GIISVEQEIK; b) AIEEANADIEVK; and c) ISGSVTVGETPVIR. S-14
15 Figure S-6. Comparison of the labeling efficiencies for 18 O-peptides produced by the IMER in 18 O-water (above) and re-dissolved in 16 O-water for 7 days (below). The sequences of the selected peptides were as follows: a) K.VLTSSARQR.L; b) K.LGEYGFQNALIVR.Y; c) R.KVPQVSTPTLVEVSR.S; d) K.DAFLGSFLYEYSR.R; e) K.YNGVFQECCQAEDK.G; and f) R.RPCFSALTPDETYVPK.A. S-15
16 Figure S-7. Evaluation of durability of the IMER. a) The sequence coverage of BSA achieved by upimer in consecutive 100 runs (10 days); b) The 18 O labeling efficiency achieved by upimer in consecutive 100 runs (10 days) S-16
17 Figure S-8. Differential proteomics analysis of Hca-F and Hca-P. a) Volcano plot of the gobal quantification of proteins in Hca-F/P cell line; b) Gene ontology analysis of quantified proteins. S-17
Supporting Information. Experimental details
Electronic Supporting Information Experimental details Chemicals and reagents Pseudoboehmite (78.4 wt% Al 2 O 3 ), phosphoric acid (85 wt%), triethylamine (TEA, 99%), tetrabutyl titanate (IV) (99%) and
More informationSupporting Information. for. Advanced Materials, adma Wiley-VCH 2006
Supporting Information for Advanced Materials, adma.200601546 Wiley-VCH 2006 69451 Weinheim, Germany Supporting Information Synthesis of Magnetic Microspheres with Immobilized Metal Ions for Enrichment
More informationProtocol for 2D-E. Protein Extraction
Protocol for 2D-E Protein Extraction Reagent 1 inside the ReadyPrep TM Sequential Extraction kit (in powder form) 50ml of deionized water is used to dissolve all the Reagent 1. The solution is known as
More informationSupporting Information
Supporting Information Realization of on-tissue protein identification by highly efficient in-situ digestion with graphene-immobilized trypsin for MALDI Imaging analysis 0 Jing Jiao, a, Aizhu Miao, b,
More informationEffective desalting and concentration of in-gel digest samples with Vivapure C18 Micro spin columns prior to MALDI-TOF analysis.
Introduction The identification of proteins plays an important role in today s pharmaceutical and proteomics research. Commonly used methods for separating proteins from complex samples are 1D or 2D gels.
More informationFunctional Magnetic Nanoparticle-Based Label Free Fluorescence Detection of Phosphorylated Species
Functional Magnetic Nanoparticle-Based Label Free Fluorescence Detection of Phosphorylated Species Cheng-Tai Chen and Yu-Chie Chen * Department of Applied Chemistry, National Chiao Tung University, Hsinchu
More informationEncapsulation of enzyme in metal ion-surfactant nanocomposites for
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2017 Supporting information for Encapsulation of enzyme in metal ion-surfactant nanocomposites for catalysis
More informationLaser desorption/ionization on the layer of graphene nanoparticles coupled with mass spectrometry for characterization of polymer
Laser desorption/ionization on the layer of graphene nanoparticles coupled with mass spectrometry for characterization of polymer Minghua Lu, a Yongquan Lai, a Guonan Chen, b and Zongwei Cai* a a Department
More information(APTES), 3- mercaptopropyltrimethoxysilane (MPTMS), tetraethyl orthosilicate (TEOS), N,Ndimethylformamide
SUPPRTING INFRMATIN Materials: Iron (II) chloride hydrate ( FeCl 2,4H 2, 99%) was purchased from Alfa Aesar ( Ward Hill, MA). Iron (III) chloride hexahydrate ( FeCl 3,6H 2, 97%), (3-aminopropyl)triethoxysilane
More informationMethods for proteome analysis of obesity (Adipose tissue)
Methods for proteome analysis of obesity (Adipose tissue) I. Sample preparation and liquid chromatography-tandem mass spectrometric analysis Instruments, softwares, and materials AB SCIEX Triple TOF 5600
More informationSERVA ICPL Kit (Cat.-No )
INSTRUCTION MANUAL SERVA ICPL Kit (Cat.-No. 39230.01) SERVA Electrophoresis GmbH Carl-Benz-Str. 7 D-69115 Heidelberg Phone +49-6221-138400, Fax +49-6221-1384010 e-mail: info@serva.de http://www.serva.de
More informationMagnetic Janus Nanorods for Efficient Capture, Separation. and Elimination of Bacteria
Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2016 Magnetic Janus Nanorods for Efficient Capture, Separation and Elimination of Bacteria Zhi-min
More informationSupplementary Materials
Supplementary Materials One-Pot Approach to Prepare Organo-silica Hybrid Capillary Monolithic Column with Intact Mesoporous Silica Nanoparticle as Building Block Shengju Liu 1,2, Jiaxi Peng 1,2, Zheyi
More informationSupporting Information
Supporting Information Highly Efficient Ionization of Phosphopeptides at Low ph by Desorption Electrospray Ionization Mass Spectrometry Ning Pan, a, b Pengyuan Liu, b Weidong Cui, c Bo Tang, a Jingmin
More informationImproved 6- Plex TMT Quantification Throughput Using a Linear Ion Trap HCD MS 3 Scan Jane M. Liu, 1,2 * Michael J. Sweredoski, 2 Sonja Hess 2 *
Improved 6- Plex TMT Quantification Throughput Using a Linear Ion Trap HCD MS 3 Scan Jane M. Liu, 1,2 * Michael J. Sweredoski, 2 Sonja Hess 2 * 1 Department of Chemistry, Pomona College, Claremont, California
More informationCollege of Life Science and Bioengineering, Beijing University of Technology, No.100, PingLeYuan, ChaoYang District, Beijing, , P.R.
Supporting information A T 1 -Mediated Nanosensor for Immunoassay Based on Activatable MnO 2 Nano-Assembly Zixin Liu, #, Yunlei Xianyu #, Wenshu Zheng #, Jiangjiang Zhang, Yunjing Luo *, Yiping Chen *,
More informationSupporting Information
Supporting Information A Low-Temperature Solid-Phase Method to Synthesize Highly Fluorescent Carbon Nitride Dots with Tunable Emission Juan Zhou, Yong Yang, and Chun-yang Zhang* Single-Molecule Detection
More informationSupporting Information
Supporting Information Coating Fe 3 O 4 Magnetic Nanoparticles with Humic Acid for High Efficient Removal of Heavy Metals in Water Jing-fu Liu, Zong-shan Zhao, and Gui-bin Jiang* State Key Laboratory of
More informationQuantitation of a target protein in crude samples using targeted peptide quantification by Mass Spectrometry
Quantitation of a target protein in crude samples using targeted peptide quantification by Mass Spectrometry Jon Hao, Rong Ye, and Mason Tao Poochon Scientific, Frederick, Maryland 21701 Abstract Background:
More informationTryptic Digest of Bovine Serum Albumin
CARE products are designed to support our worldwide customers with high-quality consumables, accessories and dedicated kits. The CARE product range is specifically optimized and certified for use with
More informationProtein Reduction, Alkylation, Digestion
Contents... 1 Reduction / Alkylation (DTT, IAA and UREA), Trypsin digestion... 2 Reagents and Materials (see Table 1)... 2 Reduction / Alkylation (DTT, IAA and UREA)... 2 Trypsin digestion:... 2 Reduction
More informationNatural montmorillonite nanosheet colloid-catalyzed hydrogen peroxide
Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2014 Supplementary Material (ESI) for RSC Advances Supplementary Information Natural montmorillonite
More informationHybrid Gold Superstructures: Synthesis and. Specific Cell Surface Protein Imaging Applications
Supporting Information Hybrid Gold Nanocube@Silica@Graphene-Quantum-Dot Superstructures: Synthesis and Specific Cell Surface Protein Imaging Applications Liu Deng, Ling Liu, Chengzhou Zhu, Dan Li and Shaojun
More informationElectronic Supplementary Information. Microwave-assisted, environmentally friendly, one-pot preparation. in electrocatalytic oxidation of methanol
Electronic Supplementary Information Microwave-assisted, environmentally friendly, one-pot preparation of Pd nanoparticles/graphene nanocomposites and their application in electrocatalytic oxidation of
More informationSupporting Online Material. On-Chip Dielectrophoretic Co-Assembly of Live Cells and. Particles into Responsive Biomaterials
Supporting Online Material On-Chip Dielectrophoretic Co-Assembly of Live Cells and Particles into esponsive Biomaterials Shalini Gupta, ossitza G. Alargova, Peter K. Kilpatrick and Orlin D. Velev* Description
More informationSupporting Information. Electrospinning-based synthesis of highly ordered mesoporous. silica fiber for lab-in-syringe enrichment of plasma peptides
This journal is The Royal Society of Chemistry 0 Supporting Information 0 Electrospinning-based synthesis of highly ordered mesoporous silica fiber for lab-in-syringe enrichment of plasma peptides Gang-Tian
More informationSelf-assembling covalent organic frameworks functionalized. magnetic graphene hydrophilic biocomposite as an ultrasensitive
Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry 2017 Electronic Supporting Information for: Self-assembling covalent organic frameworks functionalized
More informationElectronic Supplementary Information
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 217 Electronic Supplementary Information Self-repairing metal-organic hybrid complexes for reinforcing
More informationTandem mass spectra were extracted from the Xcalibur data system format. (.RAW) and charge state assignment was performed using in house software
Supplementary Methods Software Interpretation of Tandem mass spectra Tandem mass spectra were extracted from the Xcalibur data system format (.RAW) and charge state assignment was performed using in house
More informationRapid Microwave-Assisted CNBr Cleavage of Bead-Bound Peptides
Rapid Microwave-Assisted CNBr Cleavage of Bead-Bound Peptides Su Seong Lee 1,*, Jaehong Lim 1, Junhoe Cha 1, Sylvia Tan 1, James R. Heath 1,2, * 1 Institute of Bioengineering and Nanotechnology, 31 Biopolis
More informationPeptide & Protein Quantification Kit, with RED EpicoccoStab for the accurate and sensitive determination of peptides, as well proteins
Peptide & Protein Quantification Kit, with RED EpicoccoStab for the accurate and sensitive determination of peptides, as well proteins Description Catalog #: CH4191, 1 kit up to 2000 assays Name: Protein&Peptide
More informationElectronic Supplementary Information
Electronic Supplementary Information Polymer-coated spherical mesoporous silica for ph-controlled delivery of insulin Sae Rom Choi a,, Dong-jin Jang b,, Sanghyun Kim a, Sunhyung An c, Jinwoo Lee c, Euichaul
More informationThe Blue Two Photon Fluorescence Metal Cluster Probe. Precisely Marking Cell Nuclei of Two Cell Lines
Electronic Supplementary Information The Blue Two Photon Fluorescence Metal Cluster Probe Precisely Marking Cell Nuclei of Two Cell Lines Yaling Wang, a, Yanyan Cui, a, Ru Liu, a Yueteng Wei, a Xinglu
More informationElectronic supplementary information
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2018 Electronic supplementary information Heterogeneous nucleation and growth of highly crystalline
More informationSupporting Information
Supporting Information D Nanoporous Ag@BSA Composite Microspheres As Hydrogen Peroxide Sensor Quanwen Liu a, *, Ting Zhang b, Lili Yu c, Nengqin Jia c, Da-Peng Yang d * a School of Chemistry and Materials
More informationThree Dimensional Nano-assemblies of Noble Metal. Nanoparticles-Infinite Coordination Polymers as a Specific
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2014 Electronic Supplementary Information Three Dimensional Nano-assemblies of Noble Metal Nanoparticles-Infinite
More informationSupporting Information
Supporting Information Facile Synthesis of N-Doped Carbon Dots as A New Matrix for Detection of Hydroxy-Polycyclic Aromatic Hydrocarbons by Negative-Ion Matrix-Assisted Laser Desorption/Ionization Time
More informationSwitching shape of hollow layer-by-layer hydrogel microcontainers
Electronic Supplementary Information Switching shape of hollow layer-by-layer hydrogel microcontainers Veronika Kozlovskaya, William Higgins, Jun Chen and Eugenia Kharlampieva* University of Alabama at
More informationReagents. Affinity Tag (Biotin) Acid Cleavage Site. Figure 1. Cleavable ICAT Reagent Structure.
DATA SHEET Protein Expression Analysis Reagents Background The ultimate goal of proteomics is to identify and quantify proteins that are relevant to a given biological state; and to unearth networks of
More informationSupporting Information
Supporting Information Precisely Controllable Core-Shell Ag@Carbon Dots Nanoparticles: Application to in Situ Super-Sensitive Monitoring of Catalytic Reactions Jing Jin, Shoujun Zhu, Yubin Song, Hongyue
More informationfor Affinity-tunable dual-mode specific recognition of glycoproteins via boronate affinity-based controllable oriented
Electronic Supplementary Information for Affinity-tunable dual-mode specific recognition of glycoproteins via boronate affinity-based controllable oriented surface imprinting Shuangshou Wang, Jin Ye, Zijun
More informationenzymatic cascade system
Electronic Supplementary Information Fe 3 O 4 -Au@mesoporous SiO 2 microsphere: an ideal artificial enzymatic cascade system Xiaolong He, a,c Longfei Tan, a Dong Chen,* b Xiaoli Wu, a,c Xiangling Ren,
More informationChemiluminescence excited photoelectrochemistry using graphene-quantum dots nanocomposite for biosensing
Electronic Supplementary Information (ESI) for Chemical Communications This journal is (c) The Royal Society of Chemistry 2012 Chemiluminescence excited photoelectrochemistry using graphene-quantum dots
More informationColloidal Particles with Complex Microstructures via Phase Separation in Swelled Polymer Microspheres
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2017 Supporting Information Colloidal Particles with Complex Microstructures via Phase Separation in
More informationSupporting Information
Supporting Information Dynamic Interaction between Methylammonium Lead Iodide and TiO 2 Nanocrystals Leads to Enhanced Photocatalytic H 2 Evolution from HI Splitting Xiaomei Wang,, Hong Wang,, Hefeng Zhang,,
More informationFully Zwitterionic Nanoparticle Antimicrobial Agents through Tuning of Core Size and Ligand Structure
Supporting Information Fully Zwitterionic Nanoparticle Antimicrobial Agents through Tuning of Core Size and Ligand Structure Shuaidong Huo,,,, Ying Jiang,, Akash Gupta, Ziwen Jiang, Ryan Landis, Singyuk
More informationMultifunctional polyphosphazene-coated multi-walled carbon. nanotubes for the synergistic treatment of redox-responsive
Electronic Supplementary Material (ESI) for Polymer Chemistry. This journal is The Royal Society of Chemistry 2017 Supporting information for Multifunctional polyphosphazene-coated multi-walled carbon
More informationSupporting Information
Supporting Information Substrate Hydrolysis Triggered Formation of Fluorescent Gold Nanoclusters A New Platform for the Sensing of Enzyme Activity Yang Chen, 1,2,3 Huipeng Zhou, 1 Yan Wang, 1,3 Wenying
More informationElectronic Supplementary Information. Lithium-rich composite metal oxide used as SALDI-MS matrix for the determination of small biomolecules
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2014 Electronic Supplementary Information Lithium-rich composite metal oxide used as SALDI-MS matrix
More informationTaKaRa BCA Protein Assay Kit
Cat. # T9300A For Research Use TaKaRa BCA Protein Assay Kit Product Manual Table of Contents I. Description... 3 II. Components... 3 III. Storage... 3 IV. Materials Required by not Provided... 3 V. Precautions
More informationBis sulfone Reagents. Figure 1.
Bis sulfone Reagents An intact IgG molecule has four accessible inter chain disulfide bonds that can be reduced to form eight free cysteine thiols, which can serve as sites for conjugation. The reaction
More informationPd-P nanoalloys supported on porous carbon frame as efficient catalyst for benzyl alcohol oxidation
Electronic Supplementary Material (ESI) for Catalysis Science & Technology. This journal is The Royal Society of Chemistry 2018 Supporting information Pd-P nanoalloys supported on porous carbon frame as
More informationThe Power of LC MALDI: Identification of Proteins by LC MALDI MS/MS Using the Applied Biosystems 4700 Proteomics Analyzer with TOF/TOF Optics
APPLICATION NOTE TOF MS The Power of LC MALDI: Identification of Proteins by LC MALDI MS/MS Using the Applied Biosystems 4700 Proteomics Analyzer with TOF/TOF Optics Purpose The Applied Biosystems 4700
More informationSupplemental Information
Supplemental Information Neutralizing positive charges at the surface of a protein lowers its rate of amide hydrogen exchange without altering its structure or increasing its thermostability. Bryan F.
More informationSupporting Information
Supporting Information Activating Room Temperature Long Afterglow of Carbon Dots via Covalent Fixation Kai Jiang,, Yuhui Wang, Congzhong Cai, and Hengwei Lin*, Key Laboratory of Graphene Technologies and
More informationOMX-S pro Instruction Manual* Oct/07
OMX-S Instruction Manual OMX-S for rapid in-gel digestion OMX-S pro Instruction Manual* Oct/07 Part No.: 17001 Table of Contents 1. Introduction...2 2. Content and precautions...2 2.1. Content...2 2.2.
More informationSupporting Information
Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2014 Supporting Information Au nanoparticles supported on magnetically separable Fe 2 O 3 - graphene
More informationIRDye 800CW Protein Labeling Kit Low MW
IRDye 800CW Protein Labeling Kit Low MW Developed for: Aerius, and Odyssey Family of Imagers Please refer to your manual to confirm that this protocol is appropriate for the applications compatible with
More informationAnalysis of Metals, Halides, and Inorganic Ions Using Hydrophilic Interaction Chromatography
Application Note Inorganic Ions, Water Testing, Minerals, Metals, Basic Chemicals Analysis of Metals, Halides, and Inorganic Ions Using Hydrophilic Interaction Chromatography Authors Anne Mack, Adam Bivens
More informationElectronic Supplementary Information. Zhen Zheng, Gongyu Li, Chengfan Wu, Miaomiao Zhang, Yue Zhao, and Gaolin Liang*
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2017 Electronic Supplementary Information Intracellular synthesis of D-aminoluciferin for bioluminescence
More informationSupramolecular Self-Assembly of Morphology-dependent Luminescent Ag Nanoclusters
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2014 Supporting information for Supramolecular Self-Assembly of Morphology-dependent Luminescent Ag
More informationElectronic Supplementary Material (ESI) for Chemical Communications This journal is The Royal Society of Chemistry 2011
Supplementary Information for Selective adsorption toward toxic metal ions results in selective response: electrochemical studies on polypyrrole/reduced graphene oxide nanocomposite Experimental Section
More informationSupporting information for Analysis of Cellular Responses of Macrophages to. Zinc Ions and Zinc Oxide Nanoparticles: A Combined Targeted and Proteomic
Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry 2014 Supporting information for Analysis of Cellular Responses of Macrophages to Zinc Ions and Zinc
More informationSUPPORTING INFORMATION
Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry 2014 SUPPORTING INFORMATION Materials Graphite powder (SP-1 graphite) was obtained from Bay carbon.
More informationSupplementary information
Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry 2016 Supplementary information 1. Composition of the nanoparticles 2. Dynamic light scattering 3. Scanning
More informationBackbone modification of a parathyroid hormone receptor-1 antagonist/inverse agonist
SUPPORTING INFORMATION Backbone modification of a parathyroid hormone receptor-1 antagonist/inverse agonist Ross W. Cheloha, Tomoyuki Watanabe, Thomas Dean, Samuel H. Gellman*, Thomas J. Gardella* *Corresponding
More informationPurdue-UAB Botanicals Center for Age- Related Disease
Purdue-UAB Botanicals Center for Age- Related Disease MALDI-TOF Mass Spectrometry Fingerprinting Technique Landon Wilson MALDI-TOF mass spectrometry is an advanced technique for rapid protein identification
More informationSupporting Information
Supporting Information Bamboo-Like Carbon Nanotube/Fe 3 C Nanoparticle Hybrids and Their Highly Efficient Catalysis for Oxygen Reduction Wenxiu Yang a,b, Xiangjian Liu a,b, Xiaoyu Yue a,b, Jianbo Jia,
More informationThe GO was synthesized by oxidation of purified natural small graphite and graphite
Jing-He Yang, a,b Geng Sun, a Yongjun Gao, a Huabo Zhao, a Pei Tang, a Juan Tan, b Lu b and Ding Ma*,a An-Hui a Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering,
More informationSpectrum-resolved Dual-color Electrochemiluminescence Immunoassay for Simultaneous Detection of Two Targets with Nanocrystals as Tags
Supporting Information Spectrum-resolved Dual-color Electrochemiluminescence Immunoassay for Simultaneous Detection of Two Targets with Nanocrystals as Tags Guizheng Zou, *, Xiao Tan, Xiaoyan Long, Yupeng
More informationSUPPLEMENTARY INFORMATION
Methods: Poly(zwitterionic)protein conjugates offer increased stability without sacrificing binding affinity or bioactivity Andrew J. Keefe and Shaoyi Jiang Materials: α-chymotrypsin from bovine pancreas
More informationSynthesis of 2 ) Structures by Small Molecule-Assisted Nucleation for Plasmon-Enhanced Photocatalytic Activity
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2015 Electronic Supplementary Information Synthesis of Au@UiO-66(NH 2 ) Structures by Small Molecule-Assisted
More informationRing-Opening Polymerization Reaction of Polyhedral. Oligomeric Silsesquioxanes (POSSs) for Preparation of
1 2 3 Ring-Opening Polymerization Reaction of Polyhedral Oligomeric Silsesquioxanes (POSSs) for Preparation of Well-controlled 3D Skeletal Hybrid Monoliths 4 5 6 Supporting Information Hui Lin a,b, Junjie
More informationElectronic Supplementary Information
Electronic Supplementary Information Selective Diels-Alder cycloaddition on semiconducting single-walled carbon nanotubes for potential separation application Jiao-Tong Sun, Lu-Yang Zhao, Chun-Yan Hong,
More informationAdsorption of Amino Acids, Aspartic Acid and Lysine onto Iron-Oxide Nanoparticles
Supporting Information Adsorption of Amino Acids, Aspartic Acid and Lysine onto Iron-Oxide Nanoparticles Klementina Pušnik, Mojca Peterlin, Irena Kralj Cigić, Gregor Marolt, Ksenija Kogej, Alenka Mertelj,
More informationOMX-S basic Kit Instruction Manual March/09
OMX-S for rapid in-gel digestion OMX-S basic Kit Instruction Manual March/09 Table of Contents Part No.: 17002 1. Introduction...2 Content...2 Kit components...2 Storage conditions...2 Additionally required
More informationElectronic Supplementary Information for:
Electronic Supplementary Information for: 7-((5-Nitrothiophen-2-yl)methoxy)-3H-phenoxazin-3-one as a spectroscopic off-on probe for highly sensitive and selective detection of nitroreductase Zhao Li, Xinghui
More informationA graphene oxide-based AIE biosensor with high selectivity toward bovine serum albumin
This journal is The Royal Society of Chemistry 11 Electronic Supplementary Information (ESI) A graphene oxide-based AIE biosensor with high selectivity toward bovine serum albumin Xiujuan Xu, a Jing Huang,
More informationNAD + /NADH Assay [Colorimetric]
G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name NAD + /NADH Assay [Colorimetric] (Cat. #786 1539, 786 1540) think proteins! think G-Biosciences
More informationSupplementary Materials. Synthesis of Reusable Silica Nanosphere-Supported Pt(IV) Complex for. Formation of Disulfide Bonds in Peptides
Supplementary Materials Synthesis of Reusable Silica Nanosphere-Supported Pt(IV) Complex for Formation of Disulfide Bonds in Peptides Xiaonan Hou, Xiaowei Zhao, Yamei Zhang, Aiying Han, Shuying Huo*, and
More informationSupporting Information for. PNA FRET Pair Miniprobes for Quantitative. Fluorescent in Situ Hybridization to Telomeric DNA in Cells and Tissue
Supporting Information for PNA FRET Pair Miniprobes for Quantitative Fluorescent in Situ Hybridization to Telomeric DNA in Cells and Tissue Orenstein, et al Page S2... PNA synthesis procedure Page S3...
More informationBCA Protein Quantitation Kit
BCA Protein Quantitation Kit Catalog Number KA3840 100 assays Version: 01 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
More informationCarbon nanodots as peroxidase mimetics and their applications to glucose detection
Electronic Supplementary Information Carbon nanodots as peroxidase mimetics and their applications to glucose detection Wenbing Shi, a,b Qinlong Wang, a Yijuan Long, a Zhiliang Cheng, a Shihong Chen a,
More informationIn vivo monitoring of hydrogen sulfide using a cresyl violet-based ratiometric fluorescence probe
Electronic Supplementary Information for: In vivo monitoring of hydrogen sulfide using a cresyl violet-based ratiometric fluorescence probe Qiongqiong Wan, Yanchao Song, Zhao Li, Xinghui Gao and Huimin
More informationSupplementary Information. Core-Shell Silver/Polymeric Nanoparticles-Based Combinatorial Therapy against Breast Cancer In-vitro
Supplementary Information Core-Shell Silver/Polymeric Nanoparticles-Based Combinatorial Therapy against Breast Cancer In-vitro Nancy M. El-Baz 1,2, Laila Ziko 1,3, Rania Siam 1,3, Wael Mamdouh 1,2 * 1
More informationGrowth of silver nanocrystals on graphene by simultaneous reduction of graphene oxide and silver ions with a rapid and efficient one-step approach
Growth of silver nanocrystals on graphene by simultaneous reduction of graphene oxide and silver ions with a rapid and efficient one-step approach Xiu-Zhi Tang, a Zongwei Cao, b Hao-Bin Zhang, a Jing Liu
More informationSpherotech, Inc Irma Lee Circle, Unit 101, Lake Forest, Illinois PARTICLE COATING PROCEDURES
SPHERO TM Technical Note STN-1 Rev C. 041106 Introduction Currently, there are several methods of attaching biological ligands to polystyrene particles. These methods include adsorption to plain polystyrene
More informationThe cytotoxicity of gold nanoparticles is dispersitydependent
Electronic Supplementary Material (ESI) for Dalton Transactions. This journal is The Royal Society of Chemistry 2015 The cytotoxicity of gold nanoparticles is dispersitydependent Dengtong Huang, Hualu
More informationAnalysis of Illegal Dyes in Food Matrices using Automated Online Sample Preparation with LC/MS
Application Note: 56 Analysis of Illegal Dyes in Food Matrices using Automated Online Sample Preparation with LC/MS Yang Shi, Catherine Lafontaine, Matthew Berube, John Fink, François Espourteille Thermo
More informationAlkaline Phosphatase Labeling Kit-NH2
Alkaline Phosphatase Labeling Kit-NH2 Catalog Number KA0001 1 Kit Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay...
More informationSupplementary Information:
Supplementary Information: One-Step and Rapid Synthesis of Clean and Monodisperse Dendritic Pt Nanoparticles and Their High Performance Toward Methanol Oxidation and p-nitrophenol Reduction Jun Wang, Xin-Bo
More informationSupporting Information
Supporting Information NiFe-Layered Double Hydroxide Nanosheet Arrays Supported on Carbon Cloth for Highly Sensitive Detection of Nitrite Yue Ma,, Yongchuang Wang,, Donghua Xie,, Yue Gu,, Haimin Zhang,
More informationSupporting Information Ultrathin Porous Bi 5 O 7 X (X=Cl, Br, I) Nanotubes for Effective Solar Desalination
Electronic Supplementary Material (ESI) for Journal of Materials Chemistry A. This journal is The Royal Society of Chemistry 2018 Supporting Information Ultrathin Porous Bi 5 O 7 X (X=Cl, Br, I) Nanotubes
More informationSILAC and TMT. IDeA National Resource for Proteomics Workshop for Graduate Students and Post-docs Renny Lan 5/18/2017
SILAC and TMT IDeA National Resource for Proteomics Workshop for Graduate Students and Post-docs Renny Lan 5/18/2017 UHPLC peak chosen at 26.47 min LC Mass at 571.36 chosen for MS/MS MS/MS MS This is a
More informationMag Sepharose is available in the following formats (Instructions for use are included): μl TiO 2
GE Healthcare Instructions 28-9537-65 AB Mag Sepharose Mag Sepharose Mag Sepharose is available in the following formats (Instructions for use are included): 1 500 μl Mag Sepharose, 20% medium slurry 4
More informationBottom-up Optimization of SERS Hot Spots. Supplementary Information
Bottom-up Optimization of SERS Hot Spots Laura Fabris, * Department of Materials Science and Engineering, Institute for Advanced Materials Devices ad Nanotechnology, Rutgers, The State University of New
More informationOligo Click S ROTI kit for DNA labeling
USER MANUAL Oligo Click S ROTI kit for DNA labeling ROTI kit for DNA labeling Carl Roth GmbH + Co. KG Oligo Click S ROTI kit for DNA labeling For Click Chemistry labeling of up to 10 nmol oligonucleotide
More informationElectronic supplementary information
Electronic supplementary information Surface plasmon resonance enhanced upconversion luminescence in aqueous media for TNT selective detection Nina Tu and Leyu Wang* State Key Laboratory of Chemical Resource
More informationElectrogenerated Upconverted Emission from Doped Organic Nanowires
Electrogenerated Upconverted Emission from Doped Organic Nanowires Qing Li, Chuang Zhang, Jian Yao Zheng, Yong Sheng Zhao*, Jiannian Yao* Electronic Supplementary Information (ESI) 1 Experimental details
More informationOligo Click M Reload ROTI kit for DNA labeling
USER MANUAL Oligo Click M Reload ROTI kit for DNA labeling ROTI kit for DNA labeling Carl Roth GmbH + Co. KG Oligo Click M Reload ROTI kit for DNA labeling For Click Chemistry labeling of up to 100 nmol
More information