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1 ACTA SEDIMENTOLOGICA SINICA Vol. 30 No. 6 Dec ~ 60 rpm chengxing500@ 163. com P A ~ 3 4 ~ ph 16 CO 2 HCO

2 West 2 B Calothrix brerissima G. S. West Calothrix geitler Cop. Schizothrix calcicola Ag. Gom. 3 C Gloeocapsa granosa Berkeley kütz Phormidium gelatinosum Woron Oscil- latoria limosa Agarh var. limosa Aphanocapsa muscicola Menegh Will Os- 1 cillatoria Subbrevis Schm. 4 D Gloeocapsa granosa 4 A Berkeley kütz Phormidium gelatinosum B C D Woron Calothrix geitler Cop. Gloeocapsa calcicola Gardner % 1 SE Medium 1 ~ 2 h Working solution g /l NaNO g K 2 HPO 4 3H 2 O g 12 MgSO 4 7H 2 O g Aphanocapsa Gloeocapsa Calothrix Schizothrix CaCl 2 2H 2 O KH 2 PO g g Phormidium Oscillatoria NaCl Soil extract 40ml 1 A FeCl 3 6H 2 O Fe - EDTA ml Gloeocapsa minutula Gardner. A5 solution " Distilled water 1ml 958 Gloeocapsa granosa Berkeley Soil Extract kütz Aphanocapsa muscicola Menegh. Will Gloeocapsa aeruginosa Carmichael Kütz Calothrix brerissima G. S kg ml 2 h

3 NaHCO 3 ml 4 " Composition of the A5 solution Add to 100 ml of distilled water H 3 BO 3 MnCl 2 4H 2 O ZnSO 4 7H 2 O CuSO 4 5H 2 O 286mg 181mg 22mg 7. 9g NH 4 6 Mo 7 O 24 4H 2 O 3. 9mg EDTA Fe EDTA FeCl 3 6H 2 O HCl 0. 1N Na 2 EDTA 1g Distilled water 50ml FeCl 3 6H 2 O 81mg HCl 0. 1N 50ml SPX 250IC FACHB-950 FACH-898 FACHB BG11 Working solution g /l NaNO K 2 HPO 4 3H 2 O MgSO 4 7H 2 O CaCl 2 2H 2 O Citric acid Ferric ammonium citrate EDTA dinatrium-salt Na 2 CO A5 + Co solution " 1ml " Composition of the A5 + Co solution Add to ml of distilled water H 3 BO 3 MnCl 2. H 2 O ZnSO 4 7H 2 O CuSO 4 5H 2 O Na 2 MoO 4 2H 2 O Co NO 3 2 6H 2 O FACHB-254 FACHB g 1. 81g g g g g 152 mg HCO - 3 /L # 2. 5 mm $ SPX 250IC PYX 280Z B lux 14L 10D ~ 180 rpm 2 PYX 280Z B 14L 10D ~ 180 rpm d ph Ca 2 + Scanning Electrion Microscope ph Ca ph CO 2 ph CO 2 ph ph CO 2 ph 4 ph Ca SE BG ml 1 ph 200 ml ph PHS 25B SW CJ ID 2

4 OD 560 FACHB ml UV nm OD 560 log A /A 0 0 rpm 3 60 rpm 120 rpm 180 rpm lux 25 Ca mg /l Ke = ln N 1 /N 0 / t 1 - t 0 Ke ph8 N 1 N 0 d t 1 t 0 24 UV log A /A 0 t nm rpm 4 Ca 2 + FACHB-898 synechocystis sp. FACHB-248 phor- ph midium mucicola μm log A /A 0 PE5100PC 1 2 Ca FACHB Table 1 OD optical density value of FACHB-898 in r /min 3 min different water hydrodynamic condition 2. 5% 0. 1mol /L ph = Absorbency mean value mol /L 3 Days 0 lux 60 lux 120 lux 180 lux 50% ~ 100% JEOL JSM ml SPX 250IC UV FACHB-248 PHS 25B Table 2 OD optical density value of FACHB-248 in different water hydrodynamic condition GMSX 280 Days SW CJ ID PYX 280Z B FA JA FA1004N PE5100PC JEOL JSM FACHB Absorbency mean value 0 lux 60 lux 120 lux 180 lux

5 FACHB-898 synechocystis sp. FACHB-248 phormidium mucicola FACHB-898 synechocystis sp. FACHB-248 phormidium mucicola 60 rpm 1 FACHB-898 synechocystis FACHB-898 synechocystis sp. 60 rpm mucicola FACHB-898 synechocystis sp. FACHB-248 phormidium mucicola rpm 10 2 rpm FACHB-248 phormidium mucicola FACHB-898 synechocystis sp. 60rpm FACHB-248 phormidium mucicola 180 rpm 3 60 rpm FACHB-898 synechocystis sp. Ca rpm 4 3 FACHB-898 FACHB-248 Fig. 3 Cell ratio growth rate of FACHB-898 and FACHB-248 in different water hydrodynamic condition log A /A 0 t 3 sp. FACHB-248 phormidium

6 FACHB-898 synechocystis sp. Ca rpm Ca rpm Ca Table 3 Days FACHB-898 Ca 2 + Calcium Concentration of FACHB-898 in different water hydrodynamic condition Calcium Conc. mg /l mean value 0 lux 60 lux 120 lux 180 lux control Table 4 Days Calcium Concentration of FACHB-248 in different water hydrodynamic condition Ca 2 + mg /l mean value 0 lux 60 lux 120 lux 180 lux control Ca 2 + control 4 FACHB-898 Ca Fig. 4 Ca 2 + concentration curve of FACHB-898 in different water hydrodynamic condition FACHB-248 phormidium muci- 5 FACHB-248 Ca 2 + Fig. 5 Ca 2 + concentration curve of FACHB-258 in different water hydrodynamic condition cola 0 rpm Ca rpm FACHB-248 Ca 2 + control Ca FACHB-248 Ca 2 + Ca 2 + Ca ~ 60 rpm Ca rpm 3 CO 2 1 CO 2 1 CO 2 5

7 ~ 19 m /s mg /l 47 mg /l Table 5 The reference equilibrium time under two situation in different water hydrodynamic condition hrs FACHB FACHB J Zhang Yingjun Cheng Xing. The experimental study on tufa deposition J. Carsological Sinica J Zhou Zhongfa Cheng Xing. The research on tufa spray deposition J. Journal of Guizhou Normal University J Cheng Xing. Study on the structure of rimstone dams and the hydraulic conditions J. Carsological Sinica J Liu Zaizhua Li Qiang Sun Hailong et al. Diurnal variations in hydrochemistry in a travertine depositing stream at Baishuitai Yunnan 6 SW China Observations and Explanations J. Hydrogeology & Engineering 1983 Geology Fig. 6 Concentration curve in different water hydrodynamic condition from Dianzhang J Tian Youping He Fusheng. A study on biogenetic of calcareous tufa 4 0 ~ 60 rpm FACHB-898 synechocystis sp. FACHB-248 phormidium mucicola Ca 2 + References Take the tufa in Jiuzhaigou Sichuan and Huangguoshu Guizhou as an example J. Carsological Sinica Pentecost A. Growth and calcification of Calothrix-dominated oncolites from Northern England C Crick R ed. Origin Evolution and Modern Aspects of Biomineralizations in Plants and Animals. New York Plenum Press Mervyn Peart.

8 J Zhang Dian Mervyn Peart Shi Changxing et al. Natural water softening processes associated with waterfall effects in karst areas J. Acta Sedimentologica Sinica Lin Xiuli WAN Xin-nan A J Hua Hong Cao Ruiji. summary of the study on the cause of Haizi Waterfall Landscape in Jiuzhaigou Valley J. Science and Technological Information Develop- An abrupt variation event of stromatolitic microstructures in the Neoproterozoic and its origina background J. Acta Palaeontologica Sinica ment & Economy Martina Merz-Prei Robert Riding. Cyanobacterial tufa calcification in two freshwater stream ambient environment chemical thresholds and biological process J. Sedimentary Geology O J. The Blue-green Algae Calcification Test Preliminary Research on Land Facies Calcium Carbonate Deposit in Different Water Hydrodynamic Condition CHENG Xing 1 SHI Fang-hong 2 LI Ben-gang 1 ZHANG Jin-mei 1 PAN Xiang-liang 3 1. The College of Geography and Environment Guizhou Normal University Guiyang Guizhou Traffic Science Institute Guiyang Guiyang Geochemical Institute Chinese Academy of Sciences Guiyang Abstract The surface calcification is a kind of deposit on the earth which is an important deposit type of land calcium carbonate. For a long time the hydrodynamic formation cause of land calcium carbonate deposit is familiar to the researchers. The research of the biological cause of the formation is relatively fewer. In fact the biological cause should be not ignored as well especially algous deposit process. From imitating for field hydrodynamic force condition taking the blue-green algae for example the research makes a biological deposit test under the different hydrodynamic force condition. From the test we get optimum hydrodynamic condition of algae growth and their calcification rate that is under the rate of 0 ~ 60 rpm under the excessive strong the growth of blue-green algae will be restrained and reduce the deposit rate of calcium carbonate. Under the condition of waterfall hydrodynamic force is strong in calcium carbonate depositing process hydrodynamic formation cause of land calcium carbonate deposit is the lead cause Under the weaker hydrodynamic condition such as in river bed biological calcification deposit will be the lead cause. Therefore hydrodynamic-biological cause viewpoint has been proposed in the article. From the field algous samples the calcium carbonate depositing test has been done under the different water hydrodynamic conditions. The test results show that under the faster water condition most calcium carbonate will deposit very quick and fewer algae calcium carbonate will precipitate because of adverse living environment under the condition. And under the slower water condition because of being avail for algae s living more algae calcium carbonate will precipitate and few calcium carbonate will deposit under the condition without algae. Also under the motionless condition contribution of calcium carbonate deposit have been compared in the article between the algous environment and without algous environment. The article presents while without algous under the motionless condition the calcium carbonate depositing is controlled by liquid equilibrium state over saturation liquor will keep the deposition going unless the liquor is saturated. But while algous join under the motionless condition the calcium carbonate depositing may not be controlled by liquid equilibrium state algous calcium carbonate deposit will keep going even if it is saturated. So under the faster flow condition without doubt fast flow makes CO 2 in liquor overflow calcium carbonate will deposit in quantity while the contribute from algous calcium carbonate deposit is few. Under the motionless condition Mechanism of CO 2 overflowing almost disappear or weak corresponding calcium carbonate deposit is few and algous calcium carbonate deposit is main. Then in nature largely stromatolite may indicate a quiet deposit environment. Key words hydrodynamic blue-green algae calcification deposit influence

productivity. Out of the studied strains, Monoraphidium minutum and Chlorella

productivity. Out of the studied strains, Monoraphidium minutum and Chlorella Chapter-4 Isolation and screening of microalgae for carbon sequestration and its lipid content Abstract: Samples collected from Gujarat coast and from polluted habitat, were enriched for isolation and