Making Light of Photosynthesis
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- Ralf Garrett
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1 Investigating Photosynthesis using Egeria najas Making Light of Photosynthesis Kate Andrews Paul Beaumont Margaret Louis
2 Curriculum for Excellence: Sciences Experiences and Outcomes I have collaborated on investigations into the process of photosynthesis and I can demonstrate my understanding of why plants are vital to sustaining life on Earth [SCN 3-02A]
3 Curriculum for Excellence: Sciences Experiences and Outcomes Through exploring the carbon cycle, I can describe the processes involved in maintaining the balance of gases in the air, considering causes and implications of changes in the balance [SCN 4-05b]
4 Limiting factors: carbon dioxide concentration, light intensity and temperature and their impact on photosynthesis and plant growth. Analysis of limiting factors graphs. Techniques - measuring the rate of photosynthesis The effect of limiting factors on photosynthesis. Elodea/Cabomba investigations to find out about limiting factors.
5 Cabomba caroliniana Wonderful alternative to Elodea canadensis... EU list of invasive alien species... an offence in the UK to keep, cultivate, breed, transport, sell or exchange this species, or release it, intentionally or unintentionally, into the environment.
6 Egeria najas Water plant Non-native so care in disposal! Aquarium oxygenator Narrow serrated leaves easily confused with Egeria densa.
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8 Hydrogencarbonate indicator Used to measure [CO 2 ] Orange/red in air Increasingly yellow as [CO 2 ] increases Orange red magenta deep purple as [CO 2 ] decreases
9 Hydrogencarbonate indicator ph (in 0.4 increments)
10 Fun with photosynthesis 1: Compare the effect of Egeria najas on hydrogencarbonate indicator in light and dark conditions From knowledge of hydrogencarbonate indicator, make deductions about gas exchange in plants under different conditions
11 Before starting: Wash two empty Bijou bottles with a small quantity of hydrogencarbonate indicator, then discard the indicator (wash in sink) If there is any colour change, rinse again Continue until there is no colour change
12 1.Cut 2 x 3cm lengths of Egeria najas stem Add 1 piece to each of two empty Bijou bottles
13 2. Fill each bottle with hydrogencarbonate indicator
14 3,4. Cover one Bijou with black paper. Irradiate both bottles. (30-40 min)
15 Fun with Photosynthesis 2 To investigate gas evolution under different lighting conditions To contribute to the development of an understanding of why plants are vital to sustaining life on Earth
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17 1. A piece of Egeria najas equal to the length of a boiling tube. Place in the boiling tube, stem end upwards
18 2. Trim the leaves exposing the final 3 cm of the stem
19 Fill boiling tube with 1% sodium hydrogencarbonate
20 2. Cut the stem under the liquid 3-5. Play with lamp
21 6. Squeeze the bulb of a 3 cm 3 plastic pipette very tightly and extract fluid until pipette fills
22 7. Seal pipette by placing Blu-tack TM over tip
23 8,9. Cut pipette at 3 cm 3 mark, then top up any fluid lost from the weighing boat Full pipette essential!
24 10, 11. Quickly invert the full pipette and place over the stem
25 12. Irradiate for min
26 Fun with photosynthesis 1 5.What colour changes do you notice in the hydrogencarbonate indicator? Can you suggest a reason for the colour change in the - light - dark?
27 Cross-curricular opportunities
28 NASA film Watch Earth breathe
29 Cross-curricular opportunities
30 Atmospheric CO 2 measured at Mauna Loa - Keeling Curve (taken from Scripps Institution of Oceanography)
31
32 (Taken from Scripps Institution of Oceanography)
33 Planet Earth Land 30% Photosynthesis on Earth? Water 70% Land 40% Water 60%
34
35 Phytoplankton a micro view Wikipedia image
36 Phytoplankton a macro image NASA images
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light A student investigated the effect of different wavelengths of light on the rate of photosynthesis of the water plant, Cabomba. tap
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