Lesson 5: Active Transport Protein Pumps Objectives: In this lesson the student will: CELLS NOT YOUR CELL PHONE HOMEOSTASIS: LESSON 5 OVERVIEW 1. Identify how the unique structure of the cell membrane protein pumps move molecules across the cell membrane. 2. Investigate the difference between passive and active transport. 3. Identify different types of active transport 4. Investigate how the Sodium/ Potassium pump functions as it relates to homeostasis. 5. Investigate indirect active transport as it relates to homeostasis. TEKS Biology 112.34 Principles of Health Science 130.202 Health Science 130.204 Anatomy and Physiology 130.206 Pathophysiology 130.208 Principles of Agriculture 130.2 (c)1a, 2F, 2G, 2H,4B (c) 1D, 1E,1G, 2D (c) 1D, 1E (c)1a, 2F, 2G, 2H, 6A (c)1a, 2F, 2G, 2H, 4A (c)12b Vocabulary Considerations: ATP ADP Protein pump Active transport Sodium Potassium Glucose Phosphorylated Hydrolyzed Symport Antiport Content Delivery PowerPoint Presentation: Homeostasis Lesson 5 Practice Sodium/ Glucose Symport Worksheet Active Transport coloring worksheet Evaluate Pre/Post Test: Active Transport: Protein Pump
Homeostasis Lesson 5: Active Transport Pre- Test 1. What is active transport? 2. How is active transport different than passive transport? 3. What are some different types of active transport? 4. What is necessary for active transport to occur? 5. Describe how the Sodium Potassium pump functions. 6. What is a symport? 7. Give an example of a symport and describe how it works. 8. What is an antiport? Label the transport pump for A and B on the diagram 9.(A) 10.(B)
Homeostasis Lesson 5: Active Transport Test Key 9. What is active transport? Active transport is the process of moving molecules in and out of the cell with the use of ATP. 10. How is active transport different than passive transport? Active transport is different than passive in that the molecules are moving against the concentration gradient with the use of ATP. 11. What are some different types of active transport? Protein pumps Endo/exocytosis 12. What is necessary for active transport to occur? ATP is necessary for active transport to occur. 13. Describe how the Sodium Potassium pump functions. 3 intracellular Na+ molecules bind to the protein pump. ATP is hydrolyzed. When pump is phosphorylated its shape changes releasing Na+ to the outside of the cell. Once Na+ is released K+ binds to the protein. Protein loses a phosphate group causing the protein to return to original shape releasing K+ to the inside of the cell. 14. What is a symport? A symport is a type of indirect active transport where one ion is being pump against the concentration gradient while the other ion is diffusion across the membrane. Both ions are moving in the same direction, either in or out of the cell. 15. Give an example of a symport and describe how it works. Na+ Glucose pump. Allows sodium and glucose to enter the cell together at the same time. Sodium flows down its gradient while glucose is pumped up its gradient. Glucose cannot enter the cell until sodium has also bound to the protein. 16. What is an antiport? An antiport is a pump that pumps one ion in one direction which then provides the energy for the pump at the same time pump another ion in the opposite direction. Label the transport pump for A and B on the diagram 9. (A) Sodium/ Potassium pump 10. (B) Sodium/ Glucose Pump
Sodium/ Glucose Symport Worksheet Place the correct number in the blank Na+ is released inside but then is pump back out of the cell by the Na+/K+ pump. Pump opens to inside Bound Na+ signals glucose to also bind. Once both molecules are bound then protein changes shape. Loss of Na+ allows glucose to be released to the inside of cell. Na+ binds to protein Release of Glucose tells the protein to return to original shape. Color the parts of the diagram according to the chart: 1. Na+ Molecules = yellow 2.Glucose Molecules= purple 3. Protein= blue 4. Phospholipid heads= orange 5.Intracellular space= light green 6.Extracellular= light blue
Homeostasis Lesson 5: Transport Worksheet 1 2 Use the diagram to label as active or passive transport 1. 2. 3 4 5 6 Place correct number next to the process of the sodium potassium pump. Hydrolysis of ATP phosphorylates the protein pump and changes its shape 3 Na+ and 1 ATP bind to the protein pump The shape change releases Na+ outside the cell and enables K+ to bind to the pump. Release of a phosphate returns the pump to its original shape, releasing K+ to the inside of the cell. The process starts over.
Homeostasis Lesson 5: Active Transport Post- Test 17. What is active transport? 18. How is active transport different than passive transport? 19. What are some different types of active transport? 20. What is necessary for active transport to occur? 21. Describe how the Sodium Potassium pump functions. 22. What is a symport? 23. Give an example of a symport and describe how it works. 24. What is an antiport? Label the transport pump for A and B on the diagram 9.(A) 10.(B)