Chapter 12B: EUKARYOTES The Protists & Helminths. 1. Protists. Algae Protozoa. 2. Helminths. 1. Protists. A. Algae. B. Protozoa. A.

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Chapter 12B: EUKARYOTES The Protists & Helminths 1. Protists Algae Protozoa 2. Helminths 1. Protists A. Algae B. Protozoa A. Algae 1

Overview of the Algae Characteristics of algae: unicellular or multicellular eukaryotes almost all are photoautotrophs (photosynthetic) all are essentially aquatic (live in fresh or saltwater) all are capable of asexual reproduction some are capable of sexual reproduction as well **produce an estimated 80% of O 2 in the atmosphere!** Diatoms unicellular or filamentous (form multicellular filaments) have a unique cell wall structure composed of a carbohydrate called pectin & silica responsible for geometric, glass-like appearance widely distributed throughout photic zone important part of aquatic food webs Dinoflagellates what are commonly referred to as plankton unicellular algae with 2 perpendicular flagella some produce potent neurotoxins source of toxic algal blooms (e.g., red tide ) important part of the oceanic food web 2

B. Protozoa Overview of the Protozoa Characteristics of protozoa: all are unicellular eukaryotes all are heterotrophs (a few can be photosynthetic) capable of asexual reproduction (some sexual reproduction) parasitic species have complex life cycles some form protective cysts as part of their life cycles Protozoan phyla we will consider: Archaeozoa Microspora Apicomplexa Ciliophora Euglenozoa Amoebozoa Archaezoa do NOT have mitochondria have an analogous organelle called a mitosome most have multiple flagella several parasitic genera can cause human disease Trichomonas, Giardia 3

Apicomplexa non-motile obligate intracellular parasites have a unique apical complex of fibers and vacuoles that release digestive enzymes aid in penetration of host animal tissue includes species of Plasmodium responsible for the disease malaria P. vivax (inside RBCs) Plasmodium vivax Life Cycle (malaria) Malaria (pp. 696-8) Caused by protozoa in the genus Plasmodium: 4 main Plasmodium species cause malaria: P. vivax most common malaria pathogen P. falciparum most deadly malaria pathogen P. ovale & P. malariae cause milder form of malaria much like P. vivax 4

Transmission: vector transmission through the saliva of biting (female) mosquitoes (Anopholes) Signs/Symptoms: Plasmodium life cycle involves infection and destruction of RBCs periodic fever, chills anemia can be severe tissue damage due to capillary blockage Diagnosis: in most cases diagnosis is made through the microscopic examination of a blood smear Treatment: quinine is the traditional medicine for malaria quinine derivatives chloroquine & mefloquine are more commonly used today Prevention: causes death of merozoites in RBCs elimination, avoidance of mosquito vectors an truly effective vaccine has yet to be produced Ciliophora ( ciliates ) all have many small projections called cilia used for locomotion & to direct food into the cytostome ( mouth ) some have multiple nuclei have contractile vacuole to expel excess water taken in by osmosis (expelled by exocytosis) 5

Sexual Reproduction in Ciliates Most ciliates are capable of sexual reproduction by a process called conjugation: conjugating Paramecia ea produce 4 haploid micronuclei by meiosis, 3 of which disintegrate remaining micronucleus divides by mitosis (2) exchange of 1 micronucleus between cells conjugating Paramecia old & new micronuclei fuse, divide by mitosis, one of which replaces original micronucleus Euglenozoa some are photosynthetic (Euglena) have a light-sensitive eyespot & a single flagellum includes the hemoflagellates (Trypanosoma) responsible for sleeping sickness & Chagas disease Sleeping Sickness tse tse fly Chagas Disease kissing bug Trypanosoma brucei Trypanosoma cruzi 6

Amoebozoa aka amoebas have distinct form of locomotion called amoeboid movement extend cytoplasmic projections called pseudopods also used to engulf and ingest food by phagocytosis 3. Helminths Overview of the Helminths Helminths are parasitic worms found in 2 animal phyla, the Platyhelminthes (flatworms) and the Nematodes (roundworms). multicellular eukaryotic heterotrophs have complex life cycles frequently involving multiple hosts contain distinct organ systems some may be reduced or absent due to dependence on host (e.g., no digestive system, no locomotion) 7

Platyhelminthes (flatworms): typically hermaphroditic (monoecious) have a proctostome (single opening, no anus) we will look at 2 classes: Trematodes (flukes) & Cestodes (tapeworms) Nematodes (roundworms): typically dioecious (2 sexes) have complete digestive system (mouth & anus) we will look at 2 types: pinworms & hookworms Trematodes (flukes) (liver fluke) Members of this class of flatworms are all parasites associated w/particular host tissues (liver, blood, lung) can have multiple larval stages and intermediate hosts hermaphroditic (monoecious) attach to host tissue via oral and ventral suckers absorb nutrients through outer cuticle Fluke Life Cycle (blood fluke) 8

Tapeworms (cestodes) intestinal parasites scolex (head) has hooks & suckers for attachment no digestive system, absorb nutrients repeating proglottids have male & female reproductive organs (monoecious) mature proglottids detach & pass w/feces allowing transmission to other hosts Typical Tapeworm Life Cycle entire life cycle in human hosts live in large intestine females lay eggs on anus Pinworms Enterobius vermicularis a dioecious parasitic roundworm (nematode) transmitted to new hosts via eggs 9

Hookworms dioecious nematodes live, mate, lay eggs in small intestine eggs pass with feces, hatch in soil larvae enter new host through skin, pass to lungs via blood, lymph coughed up, swallowed to reach small intestine repeat life cycle cutting plates in mouth used for attaching to and removing host tissue Hookworm Life Cycle Key Terms for Chapter 12 mitosome cytostome pseudopods monoecious, dioecious scolex, proglottids Relevant Chapter Questions rvw: 11-14 MC: 6, 8-10 10