IR-4 ORNAMENTAL DATA REPORTING FORM 1. INVESTIGATOR (Name, Address, Phone#):Betsy Anderson / Dr. Michael Reding, USDA-ARS, Application Technology Research Unit, 1680 Madison Ave., Wooster, Ohio 44691 (330) 263-3898 LOCATION OF Wooster, OH TRIAL TYPE: (check one) FIELD X CONTAINER GREENHOUSE INTERIORSCAPE 2. PESTICIDE: COMMON NAME: Dinotefuran FORMULATION: 20 SG BATCH NO.: VO4C-16S6-1 PRODUCT: Dinotefuran EPA REG. NO. Not registered MFG: Valent 3. USE INFORMATION: COMMON NAME SCIENTIFIC NAME PLANT: Stonecrop Sedum Vera Jameson PEST(S): Black vine weevil SOIL TYPE OR TYPE OF POTTING MIX: Mixture of pine bark (60%), sphagnum peat (15%), compost (15%), sand (10%), and limestone. % SAND % SILT % CLAY % OM 25 ph 6.1 SEEDING DATE EMERGENCE DATE TRANSPLANTING DATE 4/15/04 PLANT OR POT SPACING 1.5ft. ROW SPACING 2 ft. POT SIZE 2 gallon PLOT SIZE 8 x 6 EXPERIMENTAL DESIGN RCB NO.OF REPS 4 4. APPLICATION PARAMETERS: TYPE OF APPLICATION: Drench NO. OF APPLICATIONS 1 APPLICATION TYPE Poured over soil surface NOZZLE TYPE/SIZE n/a NOZZLE PRESSURE n/a DELIVERY RATE n/a CALIBRATION DATE (S) n/a 5. APPLICATION SUMMARY: APPL.DATE RATES (a.i./a)* GROWTH STAGE 06/02/2004 1x = 0.75 lb. product/100gal. 2x = 1.50 lb. product/100gal. 4x = 3.00 lb. product/100gal. Vegetative PAGE 1 OF 6
IR-4 ORNAMENTAL DATA REPORTING FORM (Please type or print) 6. RAINFALL/IRRIGATION RECORDS: Container plants were watered as needed with drip irrigation. INCLUDE RAINFALL/IRRIGATION INFORMATION (See page 5 for rainfall record) 7. OTHER PESTICIDES, FERTILIZER, LIME AND ADJUVANTS USED: PRODUCT Osmocote Slow Release 18-6-26 AMOUNT 1 Tablespoon/ 2 gal. pot DATE 04/15/04_ PRODUCT Peter s 20-20-20 AMOUNT 16 oz. / pot DATE 04/15/04_ PRODUCT AMOUNT DATE 8. NARRATIVE SUMMARY OF METHODS AND RESULTS: Sedum plants were purchased as 4 bare root plants and transplanted into 2 gallon pots on 15 April. The drench treatment was applied on 2 June and four hours later each container was infested with 4 adult black vine weevils and caged to prevent the insects from leaving. The containers were put outdoors in a container nursery and irrigated as needed with a drip irrigation system. The drench treatment rates of Dinotefuran were: 1x = 0.75 lb. product / 100 gal. water or.86g. of product to 960 milliliters of water 2x = 1.50 lb. product / 100 gal. water or 1.72g. of product to 960 milliliters of water 4x = 3.00 lb. product / 100 gal. water or 3.45g. of product to 960 milliliters of water 960 milliliters of each treatment were prepared to apply 240 ml of solution to each 10 (2 gallon) pot as required in the protocols. The drench was applied to the soil surface of each of the four replications. Four infested plants were left untreated as controls. There was no phytotoxicity on any of the treated plants (page 3). Plants were evaluated for feeding damage on three dates (see photos on page 6). To determine efficacy, the containers were dumped on 18 October and the roots and soil carefully examined for black vine weevil larvae. Many of the larvae found were within the roots. The plants were destroyed in the process of examination. The feeding damage and efficacy results are on page 4. 9. GOOD RESEARCH PRACTICE STATEMENT: I acknowledge that I have read and followed the IR-4 Research protocol and completed this trial following good agricultural practice, or reported any deviations (note any changes from authorized protocol in narrative). SIGNATURE PRINCIPAL INVESTIGATOR DATE PAGE 2 OF 6
TOXICITY REPORT FORM TREATMENT RATE* ai/ REP. RATE #1 6/22/04 RATE #2 7/27/04 RATE # 3 8/20/04 RATE #4 9/15/04 CONTROL 0 1 0 0 0 0 CONTROL 0 2 0 0 0 0 CONTROL 0 3 0 0 0 0 CONTROL 0 4 0 0 0 0 CONTROL 0 MEAN 0 0 0 0 Dinotefuran 1x 1 0 0 0 0 Dinotefuran 1x 2 0 0 0 0 Dinotefuran 1x 3 0 0 0 0 Dinotefuran 1x 4 0 0 0 0 Dinotefuran 1x MEAN 0 0 0 0 Dinotefuran 2x 1 0 0 0 0 Dinotefuran 2x 2 0 0 0 0 Dinotefuran 2x 3 0 0 0 0 Dinotefuran 2x 4 0 0 0 0 Dinotefuran 2x MEAN 0 0 0 0 Dinotefuran 4x 1 0 0 0 0 Dinotefuran 4x 2 0 0 0 0 Dinotefuran 4x 3 0 0 0 0 Dinotefuran 4x 4 0 0 0 0 Dinotefuran 4x MEAN 0 0 0 0 NOTE: DEFINE MEASUREMENT OF TOXICITY, OR INDEX OF INJURY (0=NO INJURY, 10=COMPLETE KILL) No phytotoxicity was observed on any of the plants. PAGE 3 OF 6
EFFICACY REPORT FORM TREATMENT RATE ai/ REP. * Feeding damage date: 6/22 * Feeding damage date: 7/27 * Feeding damage date: 8/20 # of live larvae **Control CONTROL** 0 1 8 10 8 7 0 CONTROL 0 2 8 10 7 12 0 CONTROL 0 3 9 10 5 8 0 CONTROL 0 4 10 10 7 6 0 CONTROL 0 MEAN 8.75 10 6.75 8.25 0 Dinotefuran 1x 1 2 1 3 0 10 Dinotefuran 1x 2 1 1 1 0 10 Dinotefuran 1x 3 2 1 1 0 10 Dinotefuran 1x 4 1 1 0 0 10 Dinotefuran 1x MEAN 1.5 1 1.25 0 10 Dinotefuran 2x 1.5.5 0 0 10 Dinotefuran 2x 2.5.5.5 0 10 Dinotefuran 2x 3 1 1.5 0 10 Dinotefuran 2x 4.5 0 0 0 10 Dinotefuran 2x MEAN.625.5.25 0 10 Dinotefuran 4x 1 1.5 0 0 10 Dinotefuran 4x 2.5 0 0 0 10 Dinotefuran 4x 3.25 0 0 0 10 Dinotefuran 4x 4.5.5.5 2 7.6 Dinotefuran 4x MEAN.56.25.125 0.5 8.7 NOTE: *Feeding damage index of injury (0=No Feeding, 10= All leaves with feeding damage); Control determined by comparison to mean number of BVW larvae (8.25) in untreated plants. (**0=NOCONTROL, 10=COMPLETE CONTROL) 2004 IR-4 RAINFALL RECORD- WOOSTER, OHIO PAGE 4 OF 6
PRECIPITATION IN INCHES DAY APRIL MAY JUNE JULY AUGUST SEPTEMBER 1 0.72 0.33 0.03 0 0.01 0 2 0.13 0.7 0 0 0 0 3 0.01 0.51 0 0 0 0 4 0.14 0 0 0.11 0.08 0.44 5 0 0 0 0 0 0 6 0 0 0 0 0 0 7 0 0.28 0 0.81 0.01 0.13 8 0.08 0 0 0 0 1.36 9 0 0 0.27 0 0 0.04 10 0 0 0.6 0.01 0 0 11 0 0.17 1.41 0.02 0 0 12 0.66 0.01 0.03 0.16 0 0.01 13 0.56 0 1.02 0.01 0.11 0 14 0.11 0.1 0.53 0.1 0 0 15 0 0.47 1.31 0 0 0 16 0 0 0.01 0 0 0.01 17 0.24 0.52 0.51 0.06 0 1.36 18 0 0.73 0 0.04 0.94 0 19 0.04 0.05 0.02 0.01 0.72 0 20 0 0 0 0.31 1.82 0.01 21 0.36 2.54 0.02 0 0.04 0 22 0.6 0.08 0.01 0.02 0 0 23 0 0 0 0.01 0 0 24 0 0.27 0.38 0 0.05 0 25 0.01 0.05 0 0 0 0 26 0.05 0 0 0.6 0.05 0 27 0.03 0 0 0.06 0.15 0 28 0 0.01 0.2 0 0.51 0 29 0 0 0.01 0.01 0.38 0 30 0.26 0.39 0 0.28 0.01 0.01 31-0.7-1.0 0 - TOTAL: 4.0 7.9 6.36 3.62 4.88 3.37 PAGE 5 OF 6 Stonecrop plants (Sedum Vera Jameson ) were drenched with three rates of Dinotefuran (1x =.75 lb. product/100 gal., 2x = 1.5lb. product/100gal., 4x = 3.0lb. product/100gal.) on 2 June and infested and
PR.NO.: 25201 (formerly 23578A) DATE: 12/9/2004 caged with adult black vine weevils. Photographs of feeding damage on Sedum were taken on 3 August, 2004. The untreated plant had feeding around every leaf margin but very little damage was observed on the treated plants. CONTROL 1x 2x 4x PAGE 6 OF 6