egis Technologies Ion Pair eagents and Buffers Ion Pair HPLC greatly extends the applicability of HPLC by making the efficient octadecylsilyl columns amendable to use with ionized or ionizable samples. egis S-Series (for Cations) egis Q-Series (for Anions) The sulfonates are sodium salts that act as an anionic counterion for the separation and resolution of positively charged analytes. The sulfonates are available as ion pair concentrates: premixed 0. M solutions of alkyl sulfonates. When diluted to L with HPLC-grade water, a 0mL bottle forms a 0.00 M solution. Five 0mL Bottles 00mL Bottle Description Cat. No. Cat. No. S Pentanesulfonate 00 00 S Hexanesulfonate 00 00 S Heptanesulfonate 00 00 S8 ctanesulfonate 008 008 S Dodecanesulfonate 00 00 Method Development Kit S-Series 0mL of S (,,, 8, and ) 000 The Q-series is comprised of quaternary alkyltriethylamines that can be used for the resolution of negatively charged species. This unique set of cationic reagents was developed to complement the Sulfonate Series (S-series). The Quaternary Alkyltriethylamines are available as ion pair concentrates: premixed 0. M solutions of alkyl amines. When diluted to L with HPLC-grade water, a 0mL bottle forms a 0.00 M buffered solution. Five 0mL Bottles 00mL Bottle Description Cat. No. Cat. No. Q Pentyl (TEA) 00 00 Q Hexyl (TEA) 00 00 Q Heptyl (TEA) 00 00 Q8 ctyl (TEA) 008 008 Q Dodecyl (TEA) 00 00 Method Development Kit Description Cat. No. Q-Series 0mL of Q (,,, 8, and ) 000 Bulk Ion Pair eagents For Cations Cat. No. Description Grams 00 -Pentanesulfonate, sodium salt g 0 00g 00 -Hexanesulfonate, sodium salt g 0 00g 00 -Heptanesulfonate, sodium salt g 0 00g 008 -ctanesulfonate, sodium salt g 08 00g 00 -Dodecanesulfonate, sodium salt g 00 g For Anions 800 Tetrabutylammonium Phosphate 0mL 0.M, ph. 800 Tetrabutylammonium Phosphate 00mL 0.M, ph. Tech Tip: Guidelines to developing a successful method using ion pair reagents: Select a column endcapped DS (octadecylsilyl) is most common. Use only HPLC-grade water and chromatography grade reagents in mobile phase preparation. Choose the mobile phase components and concentrations that give the best separation. If nonionic components are present in the sample, optimize the resolution prior to attempting ionic separations. Select the appropriate ion pair series to provide the necessary counterion. Use the Q-series for acidic compounds and the S-series for basic compounds. Through a process of elimination, choose the alkyl chain length which results in the best separation. nce the reagent has been selected, adjust the ph of the mobile phase to maximize resolution. Because slight modification of ph can profoundly effect retention and selectivity, make all adjustments in small increments and monitor carefully. Ideally, the ion pair reagent concentration in the mobile phase should be 0.00M. However, small adjustments in reagent concentration may increase retention slightly and optimize the separation. For a complete list of egis reagents, please contact Chrom Tech. -800-8- in MN 9--000 www.chromtech.com sales@chromtech.com
Agilent Zorbax New! Zorbax apid esolution (HT) High Throughput Columns Up to 9% decrease in analysis time Great resolution Extended ph range, ph to 9 A better way to faster separations! All Agilent Zorbax columns are uniquely optimized for specific applications. ur newest Zorbax columns, apid esolution HT, are packed with totally porous.8µm particles. These columns are optimized for ultra-fast HPLC separations with more resolving power for small molecules. The typical particle size of traditional HPLC columns is µm, but in the past,.0 to.µm particles offered better efficiency with short column lengths. Now, our new apid esolution HT columns with.8µm particles nearly double laboratory productivity compared with columns containing.µm packings. You get even higher resolution and efficiency in very short columns ( to 0mm) ideal for sample screening, LC/MS and many routine analyses. apid esolution HT Columns,.8µm Dimensions StableBond Eclipse (mm) Format SB-C8 XDB-C8. x 0 column 99-90. x 0 cartridge 89-90 99-90. x 0 cartridge 89-90 99-90. x cartridge 89-90 99-90. x 0 column 800-90 900-90. x 0 cartridge 800-90 900-90. x 0 cartridge 800-90 900-90. x cartridge 800-90 900-90 apid esolution HT Cartridge Hardware Kit 80-90 Kit includes: () end-fitting assemblies; () 0mm holder; () 0mm holder; () mm holder A B C D Column Conditions Zorbax SB-C8: Mobile Phase: 0% 0mM Na HP, ph.8: 0% ACN Temperature: Ambient Detection: 0nm. Estradiol. Ethynylestradiol. Dienestrol. Norethindrone Flow Analysis Column ate Time Time Dimensions (ml/min) s (,) (min) Savings A.. x 0mm, µm.0.8 9. - B.. x 00mm,.µm.0.. % C.. x 0mm,.8µm.0.. 8% D.. x 0mm,.8µm.0.. 9% esolution between peaks and Zorbax Method Development (ph -) Low ph - Zorbax StableBond Start method development at low ph, where silanols on a P-HPLC column are protonated. This minimizes peak tailing by eliminating silanol/base interactions. At low ph, basic compounds are positively charged and their retention may be reduced. Acidic compounds may be protonated and have increased retention. etention times are usually stable with small changes in ph, producing a robust method. Volatile mobile phase additives, such as formic acid or trifluoroacetic acid (TFA), are often used at low ph with LC/MS. Mid ph -8 Zorbax Eclipse XDB or Bonus-P Develop methods at ph s at least ph unit above or below the pka to minimiz changes in retention with small changes in ph. Some silica surface H groups become - above ph to ; tailing interactions may be possible. Minimize interaction by selecting an end-capped column, using additives such as TEA (less desirable) or using polar-linked bonded phases. lica breakdown is prevented by innovative bonding chemistry, heavy endcapping and use of x-sil. High ph 8- Extend C8 In this region, basic compounds may be in their free base form. Increased retention and resolution of basic compounds are likely. etention changes little in this region-thus robust methods can be developed. lica breakdown is prevented by innovative bidentate column chemistry, heavy endcapping, use for x-sil and optimum mobile phase. Ammonium hydroxide is an excellent volatile mobile phase modifier at high ph. 8-800-8- in MN 9--000 www.chromtech.com sales@chromtech.com
Agilent Zorbax StableBond 80Å Long column lifetime and best reproducibility for low ph separations down to ph! Stable column chemistry allows use at high temperature and low ph without degradation Excellent first choice for method development StableBond packing materials are not endcapped in order to provide exceptional stability and to maximize lifetime and reproducibility under acidic mobile phase conditions. Zorbax StableBond columns are compatible with all common mobile phases including very high aqueous mobile phases. Polar compounds can be analyzed with excellent reproducibility and peak shape because SB-Aq columns have a hydrophilic surface that inhibits phase collapse, even with 00% aqueous mobile phases. Zorbax StableBond Column Specifications Phase ze Area Limits ange Endcapped Load SB-C8 80Å 80m /g 90 C.0-8.0 No 0% SB-C8 80Å 80m /g 80 C.0-8.0 No.% SB-C 80Å 80m /g 80 C.0-8.0 No.0% SB-CN 80Å 80m /g 80 C.0-8.0 No.0% SB-Phenyl 80Å 80m /g 80 C.0-8.0 No.% SB-Aq 80Å 80m /g 80 C.0-8.0 No Proprietary StableBond columns are designed for optimal use at low ph. At ph -8, highest column stability for all silica-based columns is obtained by operating at temperatures <0 C and using low buffer concentrations in the range of 0.0-0.0M. At mid-range ph, Eclipse XDB and Bonus-P are recommended. 80Å Zorbax StableBond Cartridge Columns Dimensions Particle (USP L) (USP L) (mm) ze (µm) SB-C8 SB-C8. x 0 998-8 9908-8. x 0 998-9 9908-9. x. 998-9908-. x 0. 89-90 89-90. x. 89-90 89-90.0 x.0, 0/pk 998-0 998-0. x 0. 800-90 800-90. x. 800-90 800-90 Cartridge holder - 0-8 0-8 Hardware kit - 80-90 80-90 requires cartridge holder 0-8 requires hardware kit 80-90 80Å Zorbax StableBond Columns Dimensions Particle (USP L) (USP L) (USP L0) (USP L) (mm) ze (µm) SB-C8 SB-C8 SB-CN SB-C SB-Phenyl SB-Aq. x 0 8809-90 8809-90 8809-90 8809-909 8809-9 8809-9. x 0 889-90 889-90 889-90 889-909 889-9 889-9. x 0 89-90 89-90 89-9. x 0. 89-90 89-90 89-90 89-9 89-9. x 00. 89-90 89-90 89-90 89-9 89-9. x. 89-90 89-90 89-90 89-9 89-9. x 0. 89-90 89-90 89-90 89-9 89-9.0 x 0 8809-0 8809-0 8809-0 8809-09 8809-.0 x 0 889-0 889-0 889-0 889-09 889-.0 x 0. 89-0 89-0 89-0 89-.0 x 00. 89-0. x 0 8800-9 8800-90 8800-90 8800-909 8800-9. x 0 809-90 809-90 809-90 809-909 809-9 809-9. x 0. 80990-90 80990-90 80990-9. x 00. 8-90 8-90 8-90 8-9 8-9. x. 8-90. x 0. 800-90 800-90 800-9.0 x 0. 800-90 800-90 800-90.0 x 0. 800-90 800-90.0 x 0. 800-90 800-90.0 x 8-90 8-90. x., /pk 8090-90 8090-9 8090-9 8090-9 8090-9 8090-9. x., /pk 8-9 8-9 8-9 8-9 8-9 8-9 Guard Hardware Kit (fits both. x. and. x. cartridges) Hardware kit - 80888-90 80888-90 80888-90 80888-90 80888-90 80888-90 -800-8- in MN 9--000 www.chromtech.com sales@chromtech.com 9
Agilent Zorbax StableBond 00Å Designed for the acidic ph mobile phases used in peptide and protein separations Improve hydrophobic peptide separations Applications: Peptides, biopharmaceuticals, hydrophobic peptides, proteins at low ph Zorbax 00StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two key reasons. First, wide-pore, 00Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the, 00SB-C8, 8899-90 00SB-C, 8899-909 00SB-C8, 8899-90 8 9 0 8 9 0 0 0 0 0 0 00SB-CN, 8899-90. Nase. Insulin. Cytochrome C. Lysozyme. Parvalbumin, 8 9 0 8 9 0 The 00SB-C8, C8, C and CN bonded phases all provide a different separation of this group of polypeptides. This adds an important parameter for quickly optimizing protein separations. The 00SB-CN column offers unique selectivity for more hydrophilic polypeptides. Column Conditions Zorbax 00SB Mobile phase: Linear gradient, -0% B in 0 min. Flow rate:.0ml/min Temperature: 0 C µg each protein. CD. Myoglobin 8. Carbonic Anhydrase 9. S-00β 0. S-00α bonded phase. Second, 00StableBond columns are unmatched in their durability at low ph, such as with the TFA containing mobile phases typically used. 00StableBond columns can also be sued with formic acid and acetic acid mobile phase modifiers. Zorbax StableBond Column Specifications Phase ze Area Limits ange Endcapped Load SB-C8 00Å m /g 90 C.0-8.0 No.8% SB-C8 00Å m /g 80 C.0-8.0 No.% SB-C 00Å m /g 80 C.0-8.0 No.% SB-CN 00Å m /g 80 C.0-8.0 No.% StableBond columns are designed for optimal use at low ph. At ph -8, highest column stability for all silica-based columns is obtained by operating at temperatures <0 C and using low buffer concentrations in the range of 0.0-0.0M. At mid-range ph, Eclipse XDB and Bonus-P are recommended. 00Å Zorbax StableBond Columns Dimensions Particle (USP L) (USP L) (USP L0) (mm) ze (µm) 00SB-C8 00SB-C8 00SB-CN SB-C. x 0 88099-90 88099-90 88099-90 88099-909. x 0 8899-90 8899-90 8899-90 8899-909. x 0 8090-90 8090-90 8090-90 8090-909. x 0. 89-90 89-90 89-90 89-909. x 00. 89-90 89-90. x 0. 89-90 89-90 89-90 89-909.0 x 0. 89-0.0 x 00. 89-0. x 0 880-90 880-90 880-90 880-909. x 0. 80-90. x 00. 8-90 8-90. x 0. 80-90 80-90.0 x 0. 80-90 80-90.0 x 0. 80-90 80-90.0 x, /pk 8-90 8-90. x., /pk 8090-9 8090-98 8090-9 8090-9. x., /pk 8-98 8-98 8-9 8-9 Guard Hardware Kit (fits both. x. and. x. cartridges) Hardware kit - 80888-90 80888-90 80888-90 80888-90 Zorbax Poroshell 00SB Columns for Bioanalytical Chemistry Porous Shell SLID CE µm The revolutionary new Poroshell µm particle combines a solid core with a 00Å porous surface layer bonded with SB-C8. The result is a highly efficient particle for rapid, high-resolution separations of proteins and other large molecules. Large and/or heterogeneous proteins have more complex interactions with the surface of the column packing and show improved peak shapes and resolution on superficially porous particles bonded with shorter phases such as C and C8. The simplified interaction of protein with the surface of the packing is the reason for improved chromatography. For very large, heterogeneous proteins, Zorbax Poroshell C or C8 columns give the best peak shape in a separation 00Å Zorbax StableBond Columns Dimensions Particle Poroshell Poroshell Poroshell (mm) ze (µm) 00SB-C8 00SB-C8 00SB-C. x 00-90 00-90 00-909.0 x 0-90 0-90 0-909. x., /pk 80-90 Guard Hardware Kit (fits both. x. and. x. cartridges) Hardware kit - 80888-90 0-800-8- in MN 9--000 www.chromtech.com sales@chromtech.com
Agilent Zorbax Eclipse XDB Long column life at mid ph with extra dense bonding (XDB) and double endcapping Complementary selectivity to StableBond, Bonus-P or Extend columns Uses Zorbax x-sil silica with uniform wall thickness for better column life at higher ph ph range: recommended to 8, maximum to 9 Zorbax Eclipse XDB Column Specifications Phase ze Area Limits ange Endcapped Load XDB-C8 80Å 80m /g 0 C.0-9.0 Double 0% XDB-C8 80Å 80m /g 0 C.0-9.0 Double.% XDB-Phenyl 80Å 80m /g 0 C.0-9.0 Double.% Zorbax Eclipse XDB Columns Dimensions Particle (USP L) (USP L) (USP L) (mm) ze (µm) XDB-C8 XDB-C8 XDB-Phenyl. x 0 9909-90 9909-90 9909-9. x 0 999-90 999-90 999-9. x 0 99-90 99-90. x 0. 99-90 99-90 99-9. x 00. 99-90 99-90. x. 99-90 99-90 99-9. x 0. 99-90 99-90 99-9.0 x 0 9909-0 9909-0 9909-.0 x 0 999-0 999-0 999-.0 x 0. 99-0 99-0 99-.0 x. 99-0. x 0 9900-90 9900-90 9900-9. x 0 909-90 909-90 909-9. x 0. 90990-90 90990-90. x 00. 9-90 9-90. x. 9-90. x 0. 900-90 900-90.0 x 0. 900-90 900-90.0 x 0. 900-90 900-90.0 x, /pk 8-9 8-9 Good Peak Shape for Acids, Bases, and Neutrals on Zorbax Eclipse XDB at Mid-pH. Uracil. Butyl Paraben. Propranolol. Naphthalene. Dipropyl Phthalate. Acenaphthene. Amitriptyline 0 0 In the mid-ph region, residual silanols can interact more strongly with analytes, resulting in asymmetric peaks. The Eclipse XDB column provides good peak shape in this difficult ph range. Column Conditions Zorbax Eclipse XDB-C8 Mobile phase: % 0mM NaH P, ph.0, % MeH Flow rate:.0ml/min Temperature: C Detection: UV nm Column Stability Testing. x., /pk 8090-9 8090-9 8090-9. x., /pk 8-9 8-9 8-9 Eclipse XDB-C8 (Sol-Type) After 8 column volumes Guard Hardware Kit (fits both. x. and. x. cartridges) Hardware kit - 80888-90 80888-90 80888-90 Zorbax Eclipse XDB Cartridge Columns Dimensions Particle (USP L) (USP L) (mm) ze (µm) XDB-C8 XDB-C8. x 0 998-8 9908-8. x 0 998-9 9908-9. x. 998-9908-. x 0. 99-90 99-90. x. 99-90 99-90.0 x.0, 0/pk 998-0 998-0.0 x. 990-. x 0. 900-90 900-90. x. 900-90 900-90 Cartridge holder - 0-8 0-8 Hardware kit - 80-90 80-90 requires cartridge holder 0-8 requires hardware kit 80-90 0 8, 0 8 0 Column:. x 0mm, µm Mobile Phase: 0% ACN, 0% 0mM phosphate buffer, ph.0 Flow rate:.ml/min Temperature: 0 C Water Symmetry C8 (l-type) After 8 column volumes. Uracil. Nortriptyline. Doxepin. Amitriptyline. Trimipramine Double endcapping, dense bonding, and the durable x-sil particles (sol-type) combine to provide long lifetime at ph.0 when compared to single endcapped sil-gel columns, such as the Waters Symmetry C8 column used here. The conditions used for this test high temperature (0 C) and high salt concentration (0mM), accelerate the dissolution of silica, causing premature failure of the sil-gel type column. -800-8- in MN 9--000 www.chromtech.com sales@chromtech.com
Agilent Zorbax Bonus-P Excellent peak shape for challenging basic compounds at low and mid ph Unique reversed-phase selectivity Novel bonding technology with embedded polar group and steric protection Usable in 00% aqueous mobile phases The Agilent Zorbax Bonus-P column has a polar amide group embedded in a long alkyl chain. This novel bonding reduces interactions between basic compounds and the silica support, improving peak shape for the most difficult basic compounds. Peak shape and column lifetime are further improbed by triple endcapping. In addition, diisopropyl side groups provide steric protection against acid hydrolysis for good lifetime at low ph. The Bonus-P column provides an alternate selectivity to C8 and C8 alkyl bonded phases. Zorbax Bonus-P Column Specifications Phase ze Area Limits ange Endcapped Load Bonus-P 80Å 80m /g 0 C.0-9.0 Triple 9.% Zorbax Bonus-P Columns Particle Bonus-P ze (µm) Cat. No.. x 0mm 8808-90. x 0mm 888-90. x 0mm. 88-90. x 00mm. 88-90. x mm. 88-90. x 0mm 88-90. x 0mm 89-90. x 00mm. 88-90. x 0mm. 800-90.0 x 0mm. 808-90.0 x 0mm. 808-90.0 x 0mm. 808-90.0 x mm, /pk 8-9 Guard Cartridges (requires hardware kit 80888-90). x.mm, /pk 8090-98. x.mm, /pk 8-98 Guard Hardware Kit (fits. and.mm ID cartridges) Hardware kit - 80888-90 Bonus-P Can Provide Alternative Selectivity to Alkyl Phases. Prometryne. Tebuthion. Atrazine. Propazine Bonus-P Alkyl-C8 Column Conditions Zorbax Bonus-P Column:. x 0mm Instrument: Agilent 00 Mobile phase: MeH: 0.% TFA (0:0) Flow rate: ml/min. Temperature: Ambient Inj.: µl UV: nm For low ph work, a TFA mobile phase is often preferred over phosphate and is compatible with LC/MS. Dacthal. Diuron. Propanil Bonus-P Provides Excellent Peak Shape for Anorectics at Mid-ange. Phentermine pka 0.. Fenfluramine pka 9.. Impurity Column Conditions Zorbax Bonus-P Columns:. x 0mm Mobile phase: mm K HP, ph./meh:acn (0:0), / Flow rate: ml/min. Inj.: µl UV: nm Anorectics ( Fen-phen ) 80888-90 guard hardware kit shown with guard cartridge and column -800-8- in MN 9--000 www.chromtech.com sales@chromtech.com
Agilent Zorbax Extend-C8 High efficiency and long life at high ph up to ph. Unique bidentate bonding and double endcapping provides high ph stability More efficiency and better peak shape than polymerbased columns Improve retention, resolution and peak shape of basic compounds High sensitivity for LC/MS separations of peptides.0e.0e.0e.0e.0e LC/MS Analysis of Angiotensin 80Å Extend-C8 AII + AIII AI Angiotensin I Max: 0889 0 0 0.. 0. min 00 000 m/z A. Acidic Conditions: A: 0.% TFA in water B: 0.08% TFA in 80% acetonitrile (ACN).0E 00 80 0 0 0 + + C8 lica Support C8 New bidentate C8-C8 bonding for Extend-C8 bonded phase The Agilent Zorbax Extend-C8 column uses a new bidentate C8- C8 column bonding technology to make high ph separations a good choice with a silica-based column. At high ph, non-charged basic compounds will not interact with the silica, so it is possible to do separations with high efficiency, superior peak shape and improved resolution. Some of the mobile phase buffer options for high ph include triethylamine, pyrrolidine, glycine, borate and ammonium hydroxide. Ammonium hydroxide at ph 0. is an excellent mobile phase modifier for the LC/MS of peptides and small molecules with improved sensitivity compared with TFA containing mobile phase at low ph. Zorbax Extend-C8 Column Specifications Phase ze Area Limits ange Endcapped Load Extend-C8 80m /g 0 C.0 -. Double.% Temperature limits are 0 C up to ph 8, 0 C for ph 8-.. 80Å or 00Å, as indicated in ordering information table below. Zorbax Extend-C8 Columns Dimensions Particle 80Å 00Å (mm) ze (µm) Extend-C8 Extend-C8. x 0 00-90 099-90. x 0 0-90 99-90. x 0 0-90. x 0. 9-90 9-90. x 00. 9-90 9-90. x. 9-90. x 0. 9-90 9-90.0 x 0. 9-0.0 x 0. 9-0. x 0 00-90. x 0 00-90. x 00. -90-90. x 0. 00-90 0-90.0 x 0. 00-90.0 x 0. 00-90.0 x 0. 00-90.0 x, /pk 8-9 Guard Cartridges (requires hardware kit 80888-90). x., /pk 8090-90 8090-9. x., /pk 8-90 8-9 Guard Hardware Kit (fits. and.mm ID cartridges) Hardware kit - 80888-90 80888-90.0E 00 Angiotensin I AIII.0E 80 + Max: AII 0.0E 0 + AI.0E 0 + 0 0 0.. 0. min 00 000 m/z B. Basic Conditions: A: 0mM NHH in water B: 0mM NHH in 80% ACN Both small and large peptides demonstrate selectivity changes at high and low ph. At high ph, due to a change in charge, all three Angiotensins can be resolved. In addition, the spectral clarity of Angiotensin I is dramatically improved at high ph with the ammounium hydroxide mobile phase. The Extend-C8 column can also be used for the analysis of small peptides at high ph. Column Conditions Zorbax 80Å Extend-C8 Column:. x 0mm, µm, 00-90 Flow rate: 0.mL/min Temperature: C Mobile phase: as indicated Gradient: -0% B in minutes LC/MS: Pos. Ion ESI-Vf 0V, Vcap. kv, N - psi, LPM, C Angiotensin I, II, III;.µL sample (0 pmol each) Angiotensin I: Asp-Arg-Val-Tyr-IIe-His-Pro-Phe-His-Leu Angiotensin I: Asp-Arg-Val-Tyr-IIe-His-Pro-Phe Angiotensin I: Arg-Val-Tyr-IIe-His-Pro-Phe Extend-C8 Provides Good Peak Shape at Low ph These basic compounds are separated on the Extend-C8 at low ph with excellent peak shape. The Extend-C8 column can be used at high and low ph. Column Conditions Zorbax Extend-C8 Column:. x 0mm, µm, 0-90 Mobile phase: 80% mm NaH P, ph.0, 0% MeH Flow rate:.0ml/min Temperature: C Detection: UV nm. Theobromine. Dimethylxanthine. Theophylline. Caffeine Pseudoephedrine and scopolamine are difficult to retain at low and mid ph. Pseudoephedrine is often analyzed by ion exchange methods. The Extend-C8 column retains these compounds in a noncharged ph form at high ph and improves resolution. -800-8- in MN 9--000 www.chromtech.com sales@chromtech.com 0 Improved etention, esolution and Peak Shape of Basic Antihistamines on Extend-C8 at High ph Column Conditions Zorbax Extend-C8 Column:. x 0mm, µm, 0-90 Mobile phase: ph : 0% 0mM Na HP, 0% MeH ph : 0% 0mM TEA, 0% MeH Flow rate:.0ml/min Temperature: Ambient Detection: UV nm. Maleate. Chlorpheniramine. Scopolamine. Triprolidine. Pseudoephedrine. Diphenhydramine. Doxylamine, 0 0 0 ph
Agilent Zorbax Columns Zorbax Gel Filtration Columns Zorbax Ion Exchange Columns apid separations and extended column life time often twice that of other size exclusion columns High recovery for most proteins (>90%) Usable with organic modifiers and denaturants ph range of.0-8.0 The silica surface of the Zorbax Gel Filtration columns is stabilized with zirconia, then coated with a monolayer diol phase providing a broad ph range of -8 a highly reproducible, hydrophilic column. Zorbax Gel Filtration Column Specifications Bonded Pore Particle MW Surface Phase ze ze ange Area GF-0 0Å µm,000-00,000 0m /g GF-0 00Å µm 0,000-900,000 0m /g Additional Specifications for Both Gel Filtration Columns Bonded Phase ph ange Flow ate Max Pressure GF-0 and GF-0.0-8.0 <.0mL/min,000 psi Zorbax ion exchange columns (0Å SAX and 00Å SCX) use Zorbax SIL porous silica microspheres as the stationary phase support; ion exchange groups are attached to a diol backbone. These columns provides highly efficient, rapid separations (pressure limit,000 psi). SCX uses aromatic sulfonic acid SAX uses a trifunctional quaternary amine Typical Applications: SCX basic water soluble compounds SAX aromatic carboxylic acids, aliphatic carboxylic acids, and sulfonic acids Zorbax Ion Exchange Columns Bonded Pore Particle Surface ph Bonded Phase ze ze Area ange Functionality SCX 00Å µm 0m /g.0 -.0 Sulfonic acid SAX 0Å µm 00m /g.0 -.0 Quaternary amine Separation of a Protein Mixture Zorbax GF-0 Column 8 0 Column Conditions Zorbax GF-0 Column: 9. x 0mm Flow rate: ml/min Eluent: 0mM NaCl/0mM KCl/ 0mM Na HP, ph.0 Detection: UV 0 nm. Mouse IgM 900,000 Da. Bovine Thyroglobulin 0,000 Da. Sweet Potato ß-Amylase 00,000 Da. Bovine Serum Albumin,000 Da. Chicken Albumin,000 Da. Bovine NAse,00 Da. Azide Da Cough-cold emedies Zorbax SCX Column Conditions Zorbax SCX Column:. x 0mm Flow rate: ml/min Eluent: 0. M NaH P, ph. Temperature: 0 C Detection: UV 0 nm. Pyrilamine. Theophylline. Glyceryl Guaicolate. Caffeine. Phenylephrine High-Speed ze Exclusion Separation Zorbax GF-0 Column BSA and BSA Dimers (duplicate injections) 0 Column Conditions Zorbax GF-0 Column: 9. x 0mm Flow rate: ml/min Eluent: PBS, ph. Detection: UV 0 nm Temperature: Ambient. BSA dimer. BSA monomer. Azide Zorbax Gel Filtration Columns Dimensions 0Å 00Å (mm) GF-0 GF-0 9. x 0 889-90 889-90. x 0 889-0 9. x, /pk 80-80-. x., /pk 8090-9 8090-9 Guard Hardware Kit Hardware kit for 9. ID 800-90 800-90 Hardware kit for. ID 80888-90 80888-90 0 Zorbax Ion Exchange Columns Dimensions (mm) SAX (0Å) SCX (00Å) 9. x 0 8809-0 8809-0. x 0 8809-0 8809-0. x 0 889-0 889-0. x 0 89-0.0 x 0 8000-0. x 0 8800-0. x 0 8000-0 Guard Cartridges, /pk (requires hardware kit 80888-90). x. 8090-90 8090-90 Guard Hardware Kit Hardware kit 80888-90 80888-90 -800-8- in MN 9--000 www.chromtech.com sales@chromtech.com
Agilent Hypersil Hypersil eversed Phase Columns Hypersil lica Normal Phase Columns Hypersil BDS is both base deactivated and endcapped to improve peak shapes of basic solutes by minimizing interaction with unreacted silanols. Hypersil DS, a very popular packing, adds a monolayer of octadecyl silane (C8) to the Hypersil lica support. Columns are endcapped to reduce silanol interaction. For less retention with similar selectivity, Hypersil MS columns provide a monolayer of dimethyloctylsilane (C8 alkyl chain). eversed Phase Cartridge Columns Cat. No. Description 99BD- BDS-C8,.0µm,.0 x mm 99BD- BDS-C8,.0µm,.0 x mm 99BD- BDS-C8,.0µm,.0 x mm 99B- BDS-C8,.0µm,.0 x 00mm 99B-8 BDS-C8,.0µm,.0 x 0mm 99BD- BDS-C8,.0µm,.0 x mm 99BD-8 BDS-C8,.0µm,.0 x 0mm 99B- BDS-C8,.0µm,.0 x mm 99BD-8 BDS-C8,.0µm,. x 0mm 99BD-9 BDS-C8,.0µm,. x 0mm 998- DS,.0µm,.0 x mm 99D- DS,.0µm,.0 x 0mm 99D- DS,.0µm,.0 x 00mm 998- DS,.0µm,.0 x mm 998-8 DS,.0µm,.0 x 0mm 99D- DS,.0µm,.0 x mm 99D-8 DS,.0µm,.0 x 0mm 998-9 DS,.0µm,. x 0mm 998-8 DS,.0µm,. x 0mm 99B8- BDS-C8,.0µm,.0 x mm 99B8-8 BDS-C8,.0µm,.0 x 0mm 99B8- BDS-C8,.0µm,.0 x mm 99B8- BDS-C8,.0µm,.0 x mm Agilent Cartridge Hardware 0-8 Agilent cartridge hardware eversed Phase Stainless Steel Columns 99BD- BDS-C8,.0µm,. x 00mm 99BD-8 BDS-C8,.0µm,. x 0mm 99BD- DS,.0µm,.0 x mm 99D- DS,.0µm,. x 00mm 99D- DS,.0µm,. x 0mm 99D- DS,.0µm,. x 00mm 99D- DS,.0µm,. x 00mm 99D- DS,.0µm,. x 00mm 99D- DS,.0µm,. x 00mm 998-8 DS,.0µm,. x 0mm 99M- MS,.0µm,. x 00mm 99M- MS,.0µm,. x 00mm 99M- MS,.0µm,. x 00mm Hypersil eversed Phase Guard Cartridge Columns 99KT-0 DS,.0µm,. x 0mm, /pk 998-0 DS,.0µm,.0 x mm, 0/pk 99KT-0 DS,.0µm,.0 x 0mm, 0/pk 99KT- MS,.0µm,. x 0mm, /pk 99KT- MS,.0µm,.0 x 0mm, /pk 99BD-0 BDS,.0µm,.0 x mm, 0/pk Agilent Guard Cartridge Hardware 9900CH-00 Cartridge hardware for.mm,.mm ID Hypersil silica columns from Agilent combine a strong, high-purity silica and Agilent s superior packing processes. The strength and reproducibility of these silica particles are also available in an amino phase, Hypersil APS, for normal phase chromatography. Normal Phase Guard Cartridge Columns Cat. No. Description 99AP- Hypersil APS,.0µm,. x 00mm 99AP- Hypersil APS,.0µm,. x 00mm 99AP- Hypersil APS,.0µm,. x 00mm 99SI- Hypersil,.0µm,. x 00mm 99SI- Hypersil,.0µm,. x 00mm 99SI- Hypersil,.0µm,. x 00mm 99SI- Hypersil,.0µm,. x 00mm Normal Phase Guard Cartridge Columns 99KT- Hypersil APS,.0µm,. x 0mm, /pk 99KT- Hypersil APS,.0µm,.0 x 0mm, /pk 99KT- Hypersil,.0µm,. x 0mm, /pk 99KT- Hypersil,.0µm,.0 x 0mm, /pk Agilent Guard Cartridge Hardware 9900CH-00 Cartridge hardware for.mm,.mm ID Column Identification Module (CIM) Attached to an LC column, the column identification module (CIM) communicates with the Agilent 00 Series control module and Agilent ChemStation through a built-in read/write device. Column usage data including serial and batch numbers, dimensions, particle size, packing material and number of injections is recorded electronically in the module (pre-recorded before shipment of Agilent s CIM HPLC columns). You can also record method, plate number, installation date, operator name and other data as needed. With constant updating, column information is always current. Automatic column tracking enhances the Agilent 00 Series features for meeting GLP, cgmp and quality standard requirements, and for achieving fast trouble free validation. Cartridge Columns with CIM Cat. No. Description 98BD- BDS-C8 w/cim,.0µm,.0 x 00mm 98BD-8 BDS-C8 w/cim,.0µm,.0 x 0mm 988- DS w/cim,.0µm,.0 x mm 988-8 DS w/cim,.0µm,.0 x 0mm Stainless Steel Columns with CIM 988- DS w/cim,.0µm,. x 00mm 988- DS w/cim,.0µm,. x 00mm -800-8- in MN 9--000 www.chromtech.com sales@chromtech.com