P-CN467E Highly sensitive quantitative estimation of genotoxic impurities from API and drug formulation using LC/MS/MS ASMS 24 TP496 Shruti Raju, Deepti Bhandarkar, Rashi Kochhar, Shailesh Damale, Shailendra Rane, Ajit Datar, Pratap Rasam, Jitendra Kelkar Shimadzu Analytical (India) Pvt. Ltd., A/B Rushabh Chambers, Makwana Road, Marol, Andheri (E), Mumbai-49, Maharashtra, India.
Introduction The toxicological assessment of Genotoxic Impurities (GTI) and the determination of acceptable limits for such impurities in Active Pharmaceutical Ingredients (API) is a difficult issue. As per European Medicines Agency (EMEA) guidance, a Threshold of Toxicological Concern (TTC) value of. µg/day intake of a genotoxic impurity is considered to be acceptable for most pharmaceuticals []. Dronedarone is a drug mainly used for indications of cardiac arrhythmias. GTI of this drug has been quantitated here. Method has been optimized for simultaneous analysis of {2-n-butyl-3-[4-(3-di-n-butylamino-propoxy)benzoyl]--nitro benzofuran}, {-amino-3-[4-(3-di-n-butylamino-propoxy)benzoyl}-2-n-but yl benzofuran} and {2-n-butyl-3-(4-hydroxy benzoyl)--nitro benzofuran}. Structures of Dronedarone and its GTI are shown in Figure. As literature references available on GTI analysis are minimal, the feature of automatic MRM optimisation in LCMS-84 makes method development process less tedious. In addition, the lowest dwell time and pause time and ultrafast polarity switching of LCMS-84 ensures uncompromised and high sensitive quantitation. N N NHS 2 Me N 2 Dronedarone N H N 2 NH 2 Figure. Structures of Dronedarone and its GTI 2
Method of Analysis Sample Preparation Preparation of and and stock solutions 2 mg of each impurity standard was weighed separately and dissolved in 2 ml of methanol to prepare stock solutions of each standard. Preparation of calibration levels GTI mix standards (, and ) at concentration levels of. ppb, ppb, ppb, ppb, 4 ppb, ppb and ppb were prepared in methanol using stock solutions of all the three standards. Preparation of blank sample 4 mg of Dronedarone powder sample was weighed and mixed with 2 ml of methanol. Mixture was sonicated to dissolve sample completely. Preparation of spiked (at ppb level) sample 4 mg of sample was weighed and spiked with 6 µl of ppm stock solution. Solution was then mixed with 2 ml of methanol. Mixture was sonicated to dissolve sample completely. LC/MS/MS Analytical Conditions Analysis was performed using Ultra High Performance Liquid Chromatography (UHPLC) Nexera coupled with LCMS-84 triple quadrupole system (Shimadzu Corporation, Japan), shown in Figure 2. Limit of GTI for Dronedarone is 2 mg/kg. However, general dosage of Dronedarone is 4 mg, hence, limit for GTI is.8 µg/4 mg. This when reconstituted in 2 ml system, gives an effective concentration of 4 ppb. For analytical method development it is desirable to have LQ at least 3 % of limit value, which in this case corresponds to ppb. The developed method gives provision for measuring GTI at much lower level. However, recovery studies have been done at ppb level. Figure 2. Nexera with LCMS-84 triple quadrupole system by Shimadzu 3
Below mentioned table shows the analytical conditions used for analysis of GTI. Table. LC/MS/MS analytical conditions Column : Shim-pack XR-DS II (7 mm L x 3 mm I.D.; 2.2 µm) Mobile phase : A:.% formic acid in water B: acetonitrile Flow rate :.3 ml/min ven temperature : 4 ºC Gradient program (B%) :. 2. min 3 (%); 2. 2. min 3-4 (%); 2. 7. min 4-6 (%); 7. 8. min 6- (%); 8.. min (%);.. min -3 (%);. 3. min 3 (%) Injection volume : µl MS interface : Electro Spray Ionization (ESI) MS analysis mode : MRM Polarity : Positive and negative MS gas flow : Nebulizing gas 2 L/min; Drying gas L/min MS temperature : Desolvation line 2 ºC; Heat block 4 ºC Note: Flow Control Valve (FCV) was used for the analysis to divert HPLC flow towards waste during elution of Dronedarone so as to prevent contamination of Mass Spectrometer. Results LC/MS/MS analysis LC/MS/MS method was developed for simultaneous quantitation of GTI mix standards. MRM transitions used for all GTI are given in Table 2. No peak was seen in diluent (methanol) at the retention times of GTI for selected MRM transitions which confirms the absence of any interference from diluent (shown in Figure 3). MRM chromatogram of GTI mix standard at ppb level is shown in Figure 4. Linearity studies were carried out using external standard calibration method. Calibration graphs of each GTI are shown in Figure. LQ was determined for each GTI based on the following criteria () % RSD for area < %, (2) % Accuracy between 8- % and (3) Signal to noise ratio (S/N) >. LQ of. ppb was achieved for and whereas ppb was achieved for. Results of accuracy and repeatability for all GTI are given in Table 3. Table 2: MRM transitions selected for all GTI Name of GTI MRM transition Retention time (min) Mode of ionization 479.>7..83 Positive ESI 9.>4..8 Positive ESI 338.2>244. 8.77 Negative ESI 4
:DRA-IB 479.>7.(+) CE: -29. 2:DRA-IA 9.>4.(+) CE: -4. 3: 338.2>244.(-) CE: 2. 7 2. 2.. 7.. min Figure 3. MRM chromatogram of diluent (methanol) :DRA-IB 479.>7.(+) CE: -29. 4 2:DRA-IA 9.>4.(+) CE: -4. 3: 338.2>244.(-) CE: 2. 3 3 2 2 479.>7. 9.>4. 338.2>244.. 2.. 7.. min Figure 4. MRM chromatogram of GTI mix standard at ppb level Area Area 7 Area R 2 -.9989 R 2 -.9943 R 2 -.9922 7 2 2. 2.. 7. Conc.. 2.. 7. Conc.. 2.. 7. Conc. Figure. Calibration graphs for GTI
Table 3: Results of accuracy and repeatability for all GTI Sr. No. Name of GTI Standard concentration (ppb) Calculated concentration from calibration graph (ppb) (n=6) % Accuracy (n=6) % RSD for area counts (n=6)..492 98.4 9..44 4.4 6.62 4.96 99.22 3..4. 2.97 4 38.36 9.9.7 49.93 99.83.8 3.7 3.7.86.994 99.4.2 4.96 98.32 2.82 2 4.96 37.63 96.63 94.8 2.43.27 48.6 97.2.4.38.4.99..486 97.2 4.88.62 6.2 6.97 4.9 98.24 2.6 3.97 99.23.3 4 37.378 93.4.37 48.8 97.4.43 96.747 96.7.9 Recovery studies For recovery studies, samples were prepared as described previously. MRM chromatogram of blank and spiked samples are shown in Figures 6 and 7 respectively. Results of recovery studies have been shown in Table 4. Recovery could not be calculated for as blank sample showed higher concentration than spiked concentration. 4 3 :DRA-IB 479.>7.(+) CE: -29. 2:DRA-IA 9.>4.(+) CE: -4. 3: 338.2>244.(-) CE: 2. 3 2 2 479.>7. 9.>4. 338.2>244.. 2.. 7.. min Figure 6. MRM chromatogram of blank sample 6
:DRA-IB 479.>7.(+) CE: -29. 2:DRA-IA 9.>4.(+) CE: -4. 3: 338.2>244.(-) CE: 2. 7 2 479.>7. 9.>4. 338.2>244.. 2.. 7.. min Figure 7. MRM chromatogram of spiked sample Table 4. Results of the recovery studies Name of Impurity Concentration of GTI mix standard spiked in blank sample (ppb) Average concentration obtained from calibration graph for blank sample (ppb) (A) (n=3) Average concentration obtained from calibration graph for spiked sample (ppb) (B) (n=3) % Recovery = (B-A)/ * 94.2 NA NA 3.279.84 79.678.24.723 9.689 Conclusion A highly sensitive method was developed for analysis of GTI of Dronedarone. Ultra high sensitivity, ultra fast polarity switching (UFswitching) enabled sensitive, selective, accurate and reproducible analysis of GTI from Dronedarone powder sample. References [] Guideline on The Limits of Genotoxic Impurities, (26), European Medicines Agency (EMEA). First Edition: June, 24 www.shimadzu.com/an/ For Research Use nly. Not for use in diagnostic procedures. The content of this publication shall not be reproduced, altered or sold for any commercial purpose without the written approval of Shimadzu. The information contained herein is provided to you "as is" without warranty of any kind including without limitation warranties as to its accuracy or completeness. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication. This publication is based upon the information available to Shimadzu on or before the date of publication, and subject to change without notice. Shimadzu Corporation, 24