SoyBase, the USDA-ARS Soybean Genetics and Genomics Database

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Transcription:

SoyBase, the USDA-ARS Soybean Genetics and Genomics Database David Grant Victoria Carollo Blake Steven B. Cannon Kevin Feeley Rex T. Nelson Nathan Weeks

SoyBase Site Map and Navigation

Video Tutorials: Using SoyBase and Community How-Tos

Search all of SoyBase for a specif ic item or a general term. All items related to search term will be returned. Tools for extracting and downloading all or subsets of the data in SoyBase. BLAST sequence similarity search against the Wm82.a2 genome sequence. The Full BLAST link allows sequence similarity searches against more than 30 selected subsets of soybean sequences. Jump directly to viewer for a specif ic Linkage Group (genetic map) or Chromosome (genome sequence). Search the SoyBase SoyCyc metabolism database. Comprehensive overview of the data, map and sequence browsers, and analysis and reporting tools at SoyBase. Short video tutorials on using SoyBase.

Gene models on Genome build Wm82.a2.v1 Chromosome Gm11 from 6216988 to 8899050 bp Gene Model Glyma.11g082800 Glyma.11g082900 Glyma.11g083000 Glyma.11g083100 Glyma.11g083200 Glyma.11g083300 Glyma.11g083400 Glyma.11g083500 Glyma.11g083600 Glyma.11g083700 Glyma.11g083800 Glyma.11g083900 Glyma.11g084000 Glyma.11g084100 Glyma.11g084200 Glyma.11g084300 Glyma.11g084400 Glyma.11g084500 Glyma.11g084600 Glyma.11g084700 Glyma.11g084800 Glyma.11g084900 Glyma.11g085000......... Start 6221273 6232129 6237429 6239967 6245591 6254033 6256998 6260432 6265374 6279503 6285020 6304911 6306788 6320131 6327560 6340163 6342649 6349265 6358863 6367696 6379315 6393085 6397113 Stop 6229354 6233067 6238491 6242268 6246466 6254848 6257711 6261499 6277820 6280405 6295194 6305972 6310937 6325155 6337749 6340882 6344632 6352603 6365321 6374677 6381377 6394280 6399993

Feature Glyma.11g082800 Glyma.11g082800 Glyma.11g082800 Glyma.11g082800 Glyma.11g082800 Glyma.11g082900 Glyma.11g082900 Glyma.11g083000 Glyma.11g083000 Glyma.11g083100 Glyma.11g083100 Glyma.11g083200 Glyma.11g083200 Glyma.11g083300 Glyma.11g083300 Glyma.11g083300 Glyma.11g083400 Glyma.11g083400 Glyma.11g083500 Glyma.11g083500 Glyma.11g083600 Glyma.11g083600 Glyma.11g083600 Glyma.11g083600 Glyma.11g083600 Glyma.11g083600 Glyma.11g083600......... Source KOG PFAM Panther Panther AT PFAM AT PFAM AT PFAM AT PFAM AT KOG PFAM AT PFAM AT PFAM AT GO GO GO PFAM PFAM PFAM Panther Annotation ID KOG2473 PF09734 PTHR13230 PTHR13230:SF5 AT3G49410.1 PF03087 AT4G35690.1 PF03087 AT4G35690.1 PF03087 AT4G35690.1 PF03087 AT4G35690.1 KOG4473 PF01988 AT3G43660.1 PF03087 AT4G35690.1 PF03087 AT4G35680.1 GO:0003677 GO:0006306 GO:0008168 PF00145 PF00385 PF01426 PTHR10629 Annotation Text RNA polymerase III transcription factor (TF)IIIC subunit RNA polymerase III transcription factor (TF)IIIC subunit GENERAL TRANSCRIPTION FACTOR IIIC, POLYPEPTIDE 5 GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 5 Transcription factor IIIC, subunit 5 Arabidopsis protein of unknown function Arabidopsis protein of unknown function (DUF241) Arabidopsis protein of unknown function Arabidopsis protein of unknown function (DUF241) Arabidopsis protein of unknown function Arabidopsis protein of unknown function (DUF241) Arabidopsis protein of unknown function Arabidopsis protein of unknown function (DUF241) Uncharacterized membrane protein VIT family Vacuolar iron transporter (VIT) family protein Arabidopsis protein of unknown function Arabidopsis protein of unknown function (DUF241) Arabidopsis protein of unknown function Arabidopsis protein of unknown function (DUF241) DNA binding DNA methylation methyltransferase activity C-5 cytosine-specif ic DNA methylase Chromo (CHRromatin Organisation MOdif ier) domain BAH domain CYTOSINE-SPECIFIC METHYLTRANSFERASE

Some Traits Analyzed by GWAS Al tolerance Chlorophyll content Chlorophyll f luorescence ABS/RC Chlorophyll f luorescence ETo/ABS Chlorophyll f luorescence ETo/TR Chlorophyll f luorescence Fv/Fm Chlorophyll f luorescence Piabs Days from f lowering to maturity Days to f lowering Days to maturity Drought tolerance Iron def ci iency chlorosis Pod weight, fresh Pods per plant SCN race 1 SCN race 3 Seed free amino acids Seed length Seed length to thickness ratio Seed length to width ratio Seed oil Seed protein Seed sucrose Seed thickness Seed weight Seed weight, fresh Seed width Seed width to thickness ratio Seed yield Seeds per plant Soybean mosaic virus

View GWAS QTL in Context of Genetic Map

View GWAS QTL in Context of Genome Sequence

Genome Browser Available for Both Wm82.a1 and Wm82.a2

SoySNP50K SNPs in Genome Browser

Markers in Genome Browser

RNA-seq Expression Data on the Genome Sequence

Show Large-scale Genome Structure Relationships

Transposable Element Insertions and Fast Neutron-induced Indels Displayed in Genome Browser

This shows the primary entry to the Fast Neutron Mutant Population page. From here mutants can be selected by sample name, gene model, locus name or indel name1, pedigree2, visual3 or biochemical4 phenotype, or sequence similarity to a query sequence5. 1 3 4 3 1 3 1 2 4 1 5

A list of gene or locus names can be submitted to determine if any are covered by an indel.

One or more FASTA sequences can be BLASTed against the indel sequences to determine if any are covered by an indel.

Genes Covered by an Indel

Selected Examples of Data Sets and Tools BLAST GO Enrichment Tool Data Downloads Searching SoyBase

Full BLAST Search

BLAST Targets at SoyBase * * * *

BLAST Results

View BLAST Alignments

View BLAST Hit in Genome Browser

View All BLAST Hits for Whole Genome

GO Term Enrichment Tool

Data Downloads

Search All of SoyBase for Cadmium

Summary of Search Results

Questions? We value your opinion!! Please take our quick six question survey using the link on the SoyBase home page or at http://www.soybase.org/survey/