[ FOOD SAFETY SOLUTIONS ] FASTER, EASIER, AND MORE ACCURATE FOOD SAFETY ANALYSES

[ FOOD SAFETY SOLUTIONS ] FASTER, EASIER, AND MORE ACCURATE FOOD SAFETY ANALYSES

Growing Worldwide Concern The safety of our food supply can no longer be taken for granted. As the world changes and populations continue to grow, so will the responsibility of laboratories to meet the increasing demand for safe food supplies. Growing Regulatory Requirements To meet these worldwide demands, the requirements of regulatory bodies are also increasing. As continual food safety monitoring is required, legislation for the testing of a variety of contaminants in food and beverages for exported, imported, and domestic consumption continues to become more rigorous. Growing Demands on Your Business It is no longer enough to differentiate and establish your brand based strictly on taste or dietary benefits. Now you must assure your customers that it is safe and has no negative health effects. Growing Laboratory Demands Today, food safety testing laboratories face numerous scientific and operational challenges meeting mandated detection limits, developing sample preparation techniques for complex matrices, increasing sample throughput, and maximizing their return on investment. Addressing these challenges requires innovative analytical solutions that will enhance laboratory effectiveness.

[ FOOD SAFETY SOLUTIONS ] THE WATERS SOLUTION Waters helps laboratories meet these challenges with technologies that are unparalleled in the industry. Our solutions deliver the sensitivity, stability, reproducibility, and versatility required to identify diverse chemical compounds, meet compliance requirements, reduce operational costs, and increase productivity. By integrating analytical standards and reagents, column and sample preparation chemistries, chromatography, mass spectrometry, and data management software, we continue to push the limits of key food safety applications. Waters is committed to developing new methods that will help you meet new and emerging legislative requirements. Look to us as a partner who delivers scientific expertise, flexible, superior service, and a commitment to your success. Sample Preparation and Consumables ACQUITY UPLC and UPLC Detectors UPLC/MS/MS UPLC/QTof MS APGC/MS Data Management Waters Global Services

SAMPLE PREPARATION AND CONSUMABLES SPE Method For The Cleanest Extracts Oasis Sample Extraction Products Oasis Solid Phase Extraction (SPE) products allow for simple and rapid sample preparation and method development. With the Oasis product line, you can expect robust SPE methods without having to endure irreproducible results and low recoveries, sorbent drying, or ph limitations. Greater capacity. Excellent stability over entire ph range. Cleanest extracts. Elimination of matrix effects. Reduction of ion suppression. Superior recovery, reproducibility, retention, and selectivity for a wide variety of compounds. Dispersive Sample Preparation for Multi-Residue Pesticide Analysis DisQuE Dispersive Sample Preparation Dispersive SPE is a simple sample preparation technique suitable for multi-residue pesticide analysis in a wide variety of food and agricultural products. One specific method which has become popular is the QuEChERS technique which was created to facilitate the rapid screening of large numbers of agricultural samples for pesticides. Easy and straightforward methods that require little training. Decrease in overall sample preparation time. Efficient, cost-effective method that can accommodate a wide variety of commodities. Improve consistency in extraction products. Improve lab efficiency and workflow. Improve quality. Conveniently packaged centrifuge tubes and foil pouches containing pre-weighed sorbents and buffers for use with both the AOAC and CEN QuEChERS methods.

[ FOOD SAFETY SOLUTIONS ] UPLC AND UPLC DETECTORS Faster Sample Turnaround and Lower Costs For Liquid Chromatography Separations ACQUITY UPLC H-Class System The Waters ACQUITY UPLC H-Class System offers the flexibility of a quaternary-based HPLC with the performance advantages of UPLC this means greater sample throughput, reduced cost per analysis, and better quality results for food testing laboratories that use traditional HPLC. Runtimes are up to 10 times faster. Seamlessly transition to UPLC separations. Maximize chromatographic resolution. Solvent consumption reduced by up to 95%. Significant reduction in method development times. Single Class and Compound Specific Analyses with High Performance Detectors ACQUITY UPLC Detectors ACQUITY UPLC optical and mass detectors enhance high-efficiency UPLC separations, allowing you to analyze a wide variety of food contaminants. The ACQUITY UPLC TUV, PDA, and Fluorescence detectors provide fast data acquisition capabilities for rapid analyses, and the sensitivity required for trace impurity detection and quantification. And with the ACQUITY QDa Detector, any analytical scientist can consistently generate the highest quality mass spectral data routinely no special training or expertise required. Apply the performance of UPLC to high volume, routine testing. Reduce instrument training time. Easily meet or exceed regulatory detection limits. Improve analytical workflow with Empower Chromatography Data (CDS) Software. Quantify compounds with no UV response at levels not amenable to or accessible by optical detection with the ACQUITY QDa Detector. Application areas include the detection of polyaromatic hydrocarbons (PAHs), pesticides, and aflatoxins. ACQUITY QDa Detector. A. 1 µg/l patulin B. 5 µg/l patulin Peak-to-peak S/N = 4 Peak-to-peak S/N = 19 Analysis using the ACQUITY UPLC H-Class System with the ACQUITY QDa Detector. Single ion recording (SIR) chromatograms of patulin spiked into extracted apple juice equivalent to A. 1 μg/l and B. 5 μg/l. The signal-to-noise ratio (calculated using peak-to-peak) is annotated on each chromatogram.

UPLC/MS/MS Multi-Class Compound Testing with Advanced Quantitative UPLC/MS/MS Xevo TQ-S The Xevo TQ-S provides the ultimate in quantitative capabilities for the analysis of food and beverage samples. This system is designed to enable uncomplicated access to superior UPLC/MS/MS performance, delivering the highest levels of sensitivity, selectivity, robustness, speed, and accuracy. RADAR Technology - Monitor matrix interferences by simultaneously monitoring full scan background data during quantitative MS/MS method development. StepWave off-axis ion source technology allows for the detection of target compounds at very low concentrations, dilution of samples to reduce matrix effects, and the use of smaller sample volumes. ScanWave Technology allows scientists to get both the very best 100 Xevo TQ-S. Acetamiprid 4.59 F9:MRM of 2 channels,es+ 223 > 126 100 Pyraclostrobin 9.79 F27:MRM of 2 channels,es+ 388.1 > 163 quantitative data as well as superior spectral MS/MS information. % % Automated instrument optimization with IntelliStart Technology. 0 100 Acetamiprid 4.59 min 0 F9:MRM of 2 channels,es+ 223 > 56.1 100 9.58 Pyraclostrobin 9.79 min F27:MRM of 2 channels,es+ 388.1 > 193.9 Search, manage, and automatically optimize methods with MassLynx workflow software tools including the Quanpedia Database. Simple dilute-and-shoot approach improves laboratory productivity by eliminating sample prep for certain beverage matrices. Minimize sample runtime and maximize the number of compounds assayed in a single method. % 0 4.200 4.400 4.600 4.800 5.000 min 0 10.00 9.51 10.37 min 9.600 9.800 10.000 10.200 10.400 Pesticide analysis with the ACQUITY UPLC I-Class System and the Xevo TQ-S. MRM chromatograms of acetamiprid and pyraclostrobin in mixed vegetable juice samples at ultra-trace levels (acetamiprid at 0.51 ppb and pyraclostrobin at 0.17 ppb). % Application areas include the detection of pesticides, veterinary drugs, mycotoxins, and marine biotoxins.

[ FOOD SAFETY SOLUTIONS ] Routine Quantitative Analyses that are Rapid, Robust, and Reliable Xevo TQD Created to reduce complexity in mass spectrometry, the Xevo TQD ensures maximum productivity with minimum effort. The advanced technology of the ACQUITY UPLC H-Class System and the robust universal ion source architecture available with the Xevo TQD ensure dependability, while providing a flexible platform to accommodate ever-changing food and beverage sample types. A cost-effective solution that everyone in the lab can use. The only system in its class that enables simultaneous acquisition of high quality quantitative and qualitative data from the widest range of experiments. ACQUITY UPLC H-Class System with Xevo TQD. IntelliStart Technology simplifies MS setup and routine operation with automated quantification workflow tools that minimize variability between different operators, departments, and laboratories. Observe MRM (multiple reaction monitoring) data and full scan background data simultaneously for faster, more robust method development. RADAR Technology provides the sensitivity and selectivity to monitor for matrix interferences, metabolites, impurities, and degradants in samples while accurately quantifying target compounds.

UPLC/Tof MS A Comprehensive, Fit-For-Purpose Screening Solution Waters Screening Platform Solution with UNIFI Integrates unsurpassed chromatography and mass spectrometry instrumentation with next-generation Informatics. Target, identify, quantify, and review using a streamlined analytical workflow that enables more accurate results across a wide range of food safety applications including pesticides, veterinary drugs, natural toxins, and food packaging contaminants. The ACQUITY UPLC I-Class System: Rapid, high-resolution separations help resolve components of interest from complex matrix interference peaks faster than ever before. Maximizes peak signal-to-noise to enhance mass spectral sensitivity. Provides the lowest carryover. The Xevo G2-S QTof: Xevo G2-S Q-Tof. The UNIFI Scientific Information System: Data acquisition, processing, and reporting functionality within a single easy-to-use Informatics platform. Facilitates data standardization so that information can be exchanged among departments and third-party partners. The scientific library allows you to make use of your prior work, as well as the data of colleagues. Analyze the broadest range of compounds in the most complex samples. StepWave ion optics allow for unsurpassed levels of robust sensitivity. MS E Technology simplifies and deconvolutes spectra, associating fragment ions with their specific precursor. Meet rigorous regulatory requirements by reducing false positives and false negatives. Analysis of antibiotics using the Waters Screening Platform Solution with UNIFI. A mix of 12 sulfonamides was spiked into a sample of bovine milk at 100 μg/l prior to extraction. The 12 sulfonamides were identified in the extracted sample using the accurate mass of molecular ions and retention time contained in the UNIFI Scientific Library. The identified components are displayed in the component plot. Information rich MS E data allows for confirmation of the individual sulfonamides using accurate mass high energy fragments obtained in the same injection. Annotated spectra and extracted ion chromatograms are displayed for all low and high energy masses identified for each component.

[ FOOD SAFETY SOLUTIONS ] Resolution in Three Dimensions SYNAPT G2-Si SYNAPT High Definition Mass Spectrometry is the combination of high-efficiency T-Wave ion mobility (IM) measurements and separations with high-performance tandem MS, enabling the differentiation of samples by size, shape and charge, as well as mass. StepWave, QuanTof, and MS E data independent acquisition combine to provide the most comprehensive and confident untargeted identification and quantification of compounds with UPLC/MS/MS, at the lowest concentration levels in complex matrices. Provides a large increase in analytical peak capacity, and delivers more information than is available with the highest chromatographic or mass resolution alone. SYNAPT G2-Si. The orthogonal dimension of gas-phase ion mobility separation allows you to take advantage of a molecule s Collision Cross-Section (CCS) to significantly enhance separation, specificity, sensitivity, and structural insight in your analysis. Singly charged up to 5x total MS peak capacity. Multiply charged up to 10x total MS peak capacity. retention UPLC x mobility T-Wave IMS x m/z QuanTof = peak capacity LC/HDMS

APGC/MS No Compromise GC/MS Atmospheric Pressure GC Atmospheric Pressure GC (APGC) adds GC capability to today s advanced MS technologies. This provides users with access to ultra-trace quantification, comprehensive qualitative MS and MS/MS, and high resolution, ion-mobility separations. Soft ionization means less fragmentation for many compounds compared with techniques such as electrospray ionization (EI). This can yield higher sensitivity and specificity, simplifying pre-cursor ion selection. Maximize uptime and asset utilization - Changeover from UPLC to GC is quick and simple, and since APGC is not a vacuum technique, equilibration times between modes of operation are kept to a minimum. APGC tandem quadrupole MS, using the Xevo TQ-S, enables detection limits at ultra-trace levels. This allows compliance with regulatory limits and the ability to inject less sample matrix. APGC with Xevo G2-S QTof enables the most complete screening of samples using both ACQUITY UPLC and GC separations. High-resolution, ion mobility separations with APGC and the SYNAPT G2-Si allow you to use the Collision Cross Section (CCS) of a molecule to provide significant enhancements in sensitivity, separation, specificity, and structural insight. Application areas include the detection of multi-residue pesticides, dioxins, and PCBs. Chlorpyrifos Molecular formula C9H 11 Cl 3 NO 3 PS Molar mass 350.59 100 97 NIST Library 197 Cl 100 APGC 351.93 349.93 29 Cl O O P S O N 50 Cl 314 % 0 125 65 258 47 109 208 286 81 15 133 153 169 244 276 349 224 10 30 50 70 90 110 130 150 170 190 210 230 250 270 290 310 330 350 353.93 167.99 323.89 355.92 323.8930 0 m/z 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 Pesticide analysis with APGC. Spectral comparison for chlorpyrifos: EI (left) and APGC (right). The EI spectrum (taken from NIST) is highly fragmented whereas the APGC spectrum shows less fragmentation and provides the [M+H] + as the base peak.

[ FOOD SAFETY SOLUTIONS ] WATERS LABORATORY INFORMATICS SOFTWARE Effective Data Management for Food Safety Analyses Empower 3 Chromatography Data Software (CDS) makes it easier than ever to run samples and produce meaningful results. Empower 3 s interface is designed to maximize your productivity, improving how you collect, process, and print chromatography data. Grows seamlessly from a single workstation to an enterprise-wide system with Empower 3 s scalable, modular architecture, which fits into the most modern, secure, HA (high availability) corporate architecture. NuGenesis 8 Platform is a comprehensive data management and workflow solution that supports the entire product lifecycle from discovery through manufacturing. This user-centric platform encompasses NuGenesis SDMS, a compliant-ready data repository, and NuGenesis ELN, a flexible analytical electronic laboratory notebook. Capabilities such as sample management, laboratory inventories, data retention and legal hold, and electronic laboratory execution methods, allow NuGenesis 8 to connect the scientific data from your laboratories to the business systems of your organization more effectively than ever before. MassLynx Software acquires, analyzes, and distributes Waters mass spectrometry information for comprehensive instrument control and data processing. To assist with both data acquisition and the transformation of data into usable results, MassLynx Software offers easy-to-use Application Managers that allow you to focus the power of MS on your laboratory's specific tasks. Paradigm Scientific Search is the next-generation of search technology for science-driven organizations. Offers a solution for accessing scientifically relevant information (chemical structures, methods, chemical reactions, chromatograms, spectra etc.), across an organization, spanning information silos, performing searches based on science objects, and adding structure to unstructured data. Waters Global Services Be Assured. Choose Waters Global Services. Waters Global Services focuses on optimizing Waters products with superior service, support, upgrades, training, and Waters Quality Parts. Proven Satisfaction For thirteen consecutive years, an independent quality auditing firm ranked Waters Global Services Best-in-Class in providing expert technical knowledge, quick resolution of system issues, and process support. 1 1 Achievement in Customer Excellence Award, CustomerSat, Inc., 2007-2013; NorthFace ScoreBoard Award, sm Omega Management Group Corporation, 2001-2006.

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