A 3 1 Shoot mss (g) B Root mss (g) C 8 Primry rnhes D 8 Seonry rnhes 1 e E 8 Shoot mss/rnhes norml low 1 F 8 8 Shoot mss/root mss 8 8 Supplementl Figure 1 Aitionl phenotypi t reore from the plnts esrie in Figure 1 A C. Petuni (wil type, 8 n mutnts) were grown in nutrient ontrolle hyroponis. After germintion the plnts were estlishe in stnr nutrient mei for weeks. The mei ws reple with either stnr ( µm P; rk green) or low ( µm P; light green) nutrient mei n groh ontinue for weeks. Grphs show phenotypi t s inite. Vlues re mens ± SEM (n = 9 1).
A Shoot mss (g) B Root mss (g) 8 norml low 1 C 1 8 Primry rnhes D 3 8 Seonry rnhes 1 E 1 8 Shoot mss/rnhes F 1 8 Shoot mss/root mss 8 8 G 8 8 Brnh ngle (men) e Angle fromvertil H 1 8 8 Brnh ngle norml low 8 norml 8 low norml low 8 Supplementl Figure 1 Noe Aitionl phenotypi t reore from the plnts esrie in Figure 1 D F. Petuni (wil type, 8 n mutnts) were grown in nutrient ontrolle hyroponis. After germintion the plnts were estlishe in stnr nutrient mei for 3 weeks. The mei ws reple with either stnr (7 µm N; rk lue) or low ( µm N; light lue) nutrient mei n groh ontinue for weeks. Grphs show phenotypi t s inite. Vlues re mens ±SEM (n = ).
A 1 Primry rnhes ef e g fg orner ege mile B Seonry rnhes 8 8 C Supplementl Figure 3 Aitionl phenotypi t reore from the plnts esrie in Figure 1 G I. Petuni plnts (wil type, 8 n mutnts) were grown on soil supplemente with fertiliser for 7 weeks t pek ensity of 1 plnt/ m. The plnts were sujete to three levels of rowe onitions for seven weeks: orner (three neighours; lue), ege (five neighours; green) or mile (eight neighours; mustr). Grphs show phenotypi t s inite. A n B show t for groh of primry n seonry rnhes (ount t). C, lyout of the experiment. Vlues re mens ±SEM (n = 11 ).
Dimeter (mm) 3 1 Hypootyl imeter Dimeter (mm) Stem imeter re fr re 8 8 Primry rnh lef numer 3 Seonry rnh lef numer 3 1 8 1 8 Primry rnh length. Seonry rnh length Totl rnh length length (m) 1 8 length (m).3..1. 8 length (m) 1 8 1 8 Primry rnh numer 8 3 1 Seonry rnh numer 8 1 Totl rnh numer 8 Supplementl Figure Aitionl phenotypi t reore from the plnts esrie in Figure 1 J L. Petuni plnts (wil type n 8 mutnts) were germinte then estlishe in hyroponis in white light for weeks. The lighting ws then supplemente with re (R:FR.3) or fr re light (R:FR.) for 3 weeks. Bright re rs (re light tretment), rk re rs (fr re light tretment). Grphs show phenotypi t s inite. Vlues re mens ±SEM (n = 8).
Supplementl Figure Photogrphs of plnts from the light n phosphte experiment. A representtive plnt from eh groh onition is shown, light onitions (R, W, FR) re inite in the top left orners n phosphte onitions re inite in the ottom left orners of eh piture.
Supplementl Figure Phylogeneti nlysis Phylogeneti nlysis of the lssii TCPs. Protein sequenes with similrity to known TCP genes were ientifie in puli tses y itertive BLAST serh n n initil lignment y using Geneious ws mnully eite. Phylogeneti reltionships were etermine using the PhyML plugin to rete mximum likelyhoo tree. Numers re perent ootstrp vlues for 1 replites. Nmes re given s two letters for the speies (At, Ariopsis thlin; Sl, Solnum lyopersium; St, Solnum tuerosum; Ph, Petuni hyri; B, Brhypoium isthyon; Os, Oryz stivum; Mt, Meigo truntulum; Ps, Pisum stivum; Pt, Populus tremul; P, Phoenix tylifer; Pp, Physomitrell ptens; Ap, Anemone pulstill; Nn, Nelumo nuifer; Zm, Ze mys) followe y TCP n numer where the gene hs een ssigne TCP numer in the literture n letter where suh n ssignment hs not yet een me. AtTCP18 n AtTCP1 re ommonly known s BRC1 n BRC respetively. SlTCP18 n AtTCP re lss I TCP proteins n were use s the outgroup.
Reltive trnsript unne...1.. CCD8 Light Qulity Reltive trnsript unne.3..1. PDR1 Light Qulity Reltive trnsript unne Reltive trnsript unne Reltive trnsript unne. 1. 1... 1. 1.... 1. 1... Supplementl Figure 7 DAD ** Light Qulity TCP1 Light Qulity TCP3 Light Qulity Reltive trnsript unne Reltive trnsript unne Reltive trnsript unne. 1. 1.... 1. 1....... MAXA Light Qulity TCP Light Qulity CDKB plus low minus Light Qulity Detils of trnsript unne hnges in response to light qulity (R:FR) in xillry u smples. Reltive trnsript unne of the genes teste in the xillry u smples from wil-type petuni trete with vrie P vilility n light qulity. For eh gene the smples re groupe y light tretment, the oloure rs representing P vilility µm P (green), µm P (lue), µm (rk lue). Vlues re mens (n = 3), with SEM. The vlues of unne for eh gene re normlise to the gretest unne of tht trnsript in ny smple. The * next to the gene nme inites the P vlue in Figure.
1 Asolute irriene (µw/m /nm) 8 7 8 9 Wvelength (nm) W R FR Supplementl Figure 8 Spetr of light for Re, white n fr re tretments The spetr of the light tretments use in the experiments presente in Figures,, n 7. The spetr were mesure using n Oen Optis USB spetroriometer n t pture using the Spetrsuite softwre following the mnufturer s instrutions (www.oenoptis.om).