Beaumont, TX 2009 North I II III IV V 1 HBK C5941 2 S49 W6 RR 3 8325 4 HBK C5025 5 HBK RR5425 6 HBK C5941 7 S49 W6 RR 8 8325 9 HBK C5025 10 HBK RR5425 11 Vernal 12 HBK RR5425 13 8360 14 8325 15 HBK C5941 16 Vernal 17 HBK RR5425 18 8360 19 8325 20 HBK C5941 21 S49 W6 RR 22 HBK RR5123 23 HBK C5941 24 HBK RR5425 25 8360 26 S49 W6 RR 27 HBK RR5123 28 HBK C5941 29 HBK RR5425 30 8360 31 8360 32 Vernal 33 HBK C5025 34 HBK RR5123 35 8325 36 8360 37 Vernal 38 HBK C5025 39 HBK RR5123 40 8325 41 HBK C5025 42 HBK C5941 43 Vernal 44 S49 W6 RR 45 HBK RR5123 46 HBK C5025 47 HBK C5941 48 Vernal 49 S49 W6 RR 50 HBK RR5123 51 8325 52 HBK C5025 53 HBK RR5123 54 Vernal 55 HBK C5025 56 8325 57 HBK C5025 58 HBK RR5123 59 Vernal 60 HBK C5025 61 HBK RR5123 62 8325 63 HBK RR5425 64 8360 65 S49 W6 RR 66 HBK RR5123 67 8325 68 HBK RR5425 69 8360 70 S49 W6 RR 71 HBK RR5425 72 8360 73 S49 W6 RR 74 HBK C5941 75 Vernal 76 HBK RR5425 77 8360 78 S49 W6 RR 79 HBK C5941 80 Vernal Treated Untreated Plot size = 4 rows, 30 inch row spacing, 32 ft long, 5 ft alley 92
Agronomic and Cultural Information Experimental design: Split-plot with main plots variety and sub plots treated or untreated for stink bugs Planting: Pulled beds in Morey silt loam soil (30 inch row width) on Feb 27. Planted test on Mar 21 (8 entries, treated and untreated, with 5 replications), general emergence on Mar 27 Plot size: Herbicide: 4 rows, 30 inch row width, trimmed to 32 ft after emergence First Rate @ 0.75 oz/a and Dual II Magnum @ 2.5 pts/a applied with 3-nozzle spray boom (110-04S nozzles, 29 gpa) Cultivation: Cultivated pre-plant on Mar 21 Cultivation on May 18 Treatments: Orthene 75S @ 1 lb ai/a applied with a 2-person hand-held spray boom (13, No. 2 cone nozzles, 50 mesh screens 18 gpa) to treated plots on Jun 3, Jun 16, Jul 13, Jul 30 and Sep 14; treated plots were sprayed a total of 5 times Sampling: Harvest: Inspected plots for flowering: no plots more mature than R1 on May 14. All plots short and no stink bugs observed in any plots. Pretreatment sweeps (15 sweeps per variety per rep) and stage of growth for each variety per rep on Jun 1 Stage of growth of each plot in rep 1 and 15 sweeps in each plot on Jun 8, Jun 19, Jul 15, Aug 3 and Sep 16 Plots not harvested due to poor seed yield and quality caused by abnormally high rainfall during and after podfill. Data analysis: Insect counts transformed using means separated by LSD. x + 0.5 ; all data analyzed by ANOVA and Discussion In general, stink bug populations were low throughout the experiment (Tables 1-14). Current treatment thresholds for stink bugs are about 5 per 15 sweeps. Populations never reached this density in untreated plots on any sampling date. As noted above, plots were not harvested due to abnormally high rainfall during and after podfill. Data suggest planting soybeans in March in SE Texas (east of Houston) is not a viable practice. 93
Table 1. Total (nymphs + adults) stink bugs (redbanded, southern green and brown) per 15 sweeps in soybean host plant resistance. Beaumont, TX. 2009. Variety Trt a PRE b Jun 8 Jun 19 Jul 15 Aug 3 Sep 16 8325 T 2.2 0.2 0.4 0.8 1.4 0.8 U 0 1.0 0.8 1.4 1.2 1.2 8360 T 0.2 0.6 0.8 0.8 2.0 0.8 U 0 0.8 0.6 2.6 0.6 1.0 HBK C5025 T 0.2 0.6 1.8 1.8 1.8 1.2 U 0 1.2 1.6 1.6 3.8 1.2 HBK C5941 T 1.6 0.4 1.8 2.4 2.4 2.0 U 0 0.6 1.0 1.4 2.2 2.4 HBK RR5123 T 1.8 0.2 1.0 2.0 3.6 1.0 U 0 0.2 1.0 1.8 1.8 1.0 HBK RR5425 T 0.8 0.2 2.4 2.0 3.6 1.0 U 0 0.4 1.6 1.0 1.8 1.6 S49 W6 RR T 2.0 0.6 3.2 1.6 1.0 0.8 U 0 2.2 1.0 0.2 2.0 0.4 Vernal T 2.0 0.4 0 0.6 2.8 1.2 U 0 1.4 2.2 2.2 3.2 1.2 b PRE = pretreatment sweeps Table 2. Statistical analysis of data in Table 1. PRE a Jun 8 Jun 19 Jul 15 Aug 3 Sep 16 8325 1.1 0.6 0.6 1.1 1.3 1.0 8360 0.1 0.7 0.7 1.7 1.3 0.9 HBK C5025 0.1 0.9 1.7 1.7 2.8 1.2 HBK C5941 0.8 0.5 1.4 1.9 2.3 2.2 HBK RR5123 0.9 0.2 1.0 1.9 2.7 1.0 HBK RR5425 0.4 0.3 2.0 1.5 2.7 1.3 S49 W6 RR 1.0 1.4 2.1 0.9 1.5 0.6 Vernal 1.0 0.9 1.1 1.4 3.0 1.2 Treated b 1.4 a 0.4 b 1.4 1.5 2.3 1.1 Untreated 0 b 1.0 a 1.2 1.5 2.1 1.3 P = 0.2931 0.7865 0416 0.2719 0.4206 0.9816 a PRE = pretreatment sweeps Means in a column followed by the same or no letter are not significantly different (P = 0.05, ANOVA and LSD) 94
Table 3. Mean stink bug data (number per 15 sweeps) on Jun 1 (pretreatment) for soybean host plant resistance. Beaumont, TX. 2009. 8325 T 1.6 0 1.6 0 0 0 0.6 0 0.6 8360 T 0 0 0 0 0 0 0.2 0 0.2 HBK C5025 T 0.2 0 0.2 0 0 0 0 0 0 HBK C5941 T 1.0 0 1.0 0.4 0 0.4 0.2 0 0.2 HBK RR5123 T 0.6 0 0.6 0 0 0 1.2 0 1.2 HBK RR5425 T 0.8 0 0.8 0 0 0 0 0 0 S49 W6 RR T 1.6 0 1.6 0.2 0 0.2 0.2 0 0.2 Vernal T 1.2 0 1.2 0.4 0 0.4 0.4 0 0.4 Table 4. Statistical analysis of data from Table 3. Beaumont, TX. 2009. 8325 0.8 0 0.8 0 0 0 0.3 0 0.3 8360 0 0 0 0 0 0 0.1 0 0.1 HBK C5025 0.1 0 0.1 0 0 0 0 0 0 HBK C5941 0.5 0 0.5 0.2 0 0.2 0.1 0 0.1 HBK RR5123 0.3 0 0.3 0 0 0 0.6 0 0.6 HBK RR5425 0.4 0 0.4 0 0 0 0 0 0 S49 W6 RR 0.8 0 0.8 0.1 0 0.1 0.1 0 0.1 Vernal 0.6 0 0.6 0.2 0 0.2 0.2 0 0.2 Treated b 0.9 a 0 0.9 a 0.1 0 0.1 0.4 a 0 0.4 a Untreated 0 b 0 0 b 0 0 0 0 b 0 0 b P = 0.3075 --- 0.3075 0.5640 --- 0.5640 0.1736 --- 0.1736 Means in a column followed by the same or no letter are not significantly different (P = 0.05, ANOVA and LSD) 95
Table 5. Mean stink bug data (number per 15 sweeps) on Jun 8 for soybean host plant resistance. Beaumont, TX. 2009. 8325 T 0 0 0 0 0 0 0.2 0 0.2 U 0.6 0 0.6 0.2 0 0.2 0.2 0 0.2 8360 T 0.2 0 0.2 0 0 0 0.4 0 0.4 U 0.2 0 0.2 0.6 0 0.6 0 0 0 HBK C5025 T 0.2 0 0.2 0.2 0 0.2 0.2 0 0.2 U 0.6 0 0.6 0 0 0 0.6 0 0.6 HBK C5941 T 0.2 0 0.2 0 0 0 0.2 0 0.2 U 0.4 0 0.4 0 0 0 0.2 0 0.2 HBK RR5123 T 0.2 0 0.2 0 0 0 0 0 0 U 0.2 0 0.2 0 0 0 0 0 0 HBK RR5425 T 0.2 0 0.2 0 0 0 0 0 0 U 0.2 0 0.2 0.2 0 0.2 0 0 0 S49 W6 RR T 0.4 0 0.4 0.2 0 0.2 0 0 0 U 1.6 0 1.6 0.4 0 0.4 0.2 0 0.2 Vernal T 0.4 0 0.4 0 0 0 0 0 0 U 0.8 0 0.8 0.2 0 0.2 0.4 0 0.4 Table 6. Statistical analysis of data from Table 5. Beaumont, TX. 2009. 8325 0.3 0 0.3 0.1 0 0.1 0.2 0 0.2 8360 0.2 0 0.2 0.3 0 0.3 0.2 0 0.2 HBK C5025 0.4 0 0.4 0.1 0 0.1 0.4 0 0.4 HBK C5941 0.3 0 0.3 0 0 0 0.2 0 0.2 HBK RR5123 0.2 0 0.2 0 0 0 0 0 0 HBK RR5425 0.2 0 0.2 0.1 0 0.1 0 0 0 S49 W6 RR 1.0 0 1.0 0.3 0 0.3 0.1 0 0.1 Vernal 0.6 0 0.6 0.1 0 0.1 0.2 0 0.2 Treated b 0.2 b 0 0.2 b 0.1 0 0.1 0.1 0 0.1 Untreated 0.6 a 0 0.6 a 0.2 0 0.2 0.2 0 0.2 P = 0.6813 --- 0.6813 0.7084 --- 0.7084 0.5391 --- 0.5391 Means in a column followed by the same or no letter are not significantly different (P = 0.05, ANOVA and LSD) 96
Table 7. Mean stink bug data (number per 15 sweeps) on Jun 19 for soybean host plant resistance. Beaumont, TX. 2009. 8325 T 0.2 0 0.2 0.2 0 0.2 0 0 0 U 0.6 0.2 0.8 0 0 0 0 0 0 8360 T 0.6 0.2 0.8 0 0 0 0 0 0 U 0 0 0 0.2 0 0.2 0.4 0 0.4 HBK C5025 T 1.6 0 1.6 0 0 0 0.2 0 0.2 U 0.8 0 0.8 0.4 0 0.4 0.4 0 0.4 HBK C5941 T 0.4 0 0.4 0.4 0 0.4 1.0 0 1.0 U 0.6 0 0.6 0 0 0 0.4 0 0.4 HBK RR5123 T 0.2 0 0.2 0.4 0 0.4 0.4 0 0.4 U 0.6 0 0.6 0.2 0 0.2 0.2 0 0.2 HBK RR5425 T 1.4 0.4 1.8 0.2 0 0.2 0.4 0 0.4 U 1.0 0 1.0 0 0 0 0.6 0 0.6 S49 W6 RR T 2.2 0 2.2 0.4 0.4 0.8 0.2 0 0.2 U 0.2 0.2 0.4 0.2 0 0.2 0.4 0 0.4 Vernal T 0 0 0 0 0 0 0 0 0 U 0.8 0 0.8 0.4 0 0.4 1.0 0 1.0 Table 8. Statistical analysis of data from Table 7. Beaumont, TX. 2009. 8325 0.4 0.1 0.5 ab 0.1 0 b 0.1 0 0 0 8360 0.3 0.1 0.4 b 0.1 0 b 0.1 0.2 0 0.2 HBK C5025 1.2 0 1.2 a 0.2 0 b 0.2 0.3 0 0.3 HBK C5941 0.5 0 0.5 ab 0.2 0 b 0.2 0.7 0 0.7 HBK RR5123 0.4 0 0.4 b 0.3 0 b 0.3 0.3 0 0.3 HBK RR5425 1.2 0.2 1.4 a 0.1 0 b 0.1 0.5 0 0.5 S49 W6 RR 1.2 0.1 1.3 a 0.3 0.2 a 0.5 0.3 0 0.3 Vernal 0.4 0 0.4 b 0.2 0 b 0.2 0.5 0 0.5 Treated b 0.8 0.1 0.9 0.2 0.1 0.3 0.3 0 0.3 Untreated 0.6 0.1 0.6 0.2 0 0.2 0.4 0 0.4 P = 0.0486 0.1305 0.0418 0.3455 0.0272 0.1623 0.2525 --- 0.2525 Means in a column followed by the same or no letter are not significantly different (P = 0.05, ANOVA and LSD) 97
Table 9. Mean stink bug data (number per 15 sweeps) on Jul 15 for soybean host plant resistance. Beaumont, TX. 2009. 8325 T 0.8 0 0.8 0 0 0 0 0 0 U 1.4 0 1.4 0 0 0 0 0 0 8360 T 0.6 0 0.6 0 0 0 0.2 0 0.2 U 2.4 0 2.4 0 0 0 0.2 0 0.2 HBK C5025 T 1.6 0 1.6 0 0 0 0.2 0 0.2 U 1.4 0 1.4 0 0 0 0.2 0 0.2 HBK C5941 T 2.4 0 2.4 0 0 0 0 0 0 U 1.2 0 1.2 0 0 0 0.2 0 0.2 HBK RR5123 T 1.6 0 1.6 0.2 0 0.2 0.2 0 0.2 U 1.6 0 1.6 0.2 0 0.2 0 0 0 HBK RR5425 T 1.6 0 1.6 0.2 0 0.2 0.2 0 0.2 U 0.8 0 0.8 0 0 0 0.2 0 0.2 S49 W6 RR T 1.2 0 1.2 0 0 0 0.4 0 0.4 U 0.2 0 0.2 0 0 0 0 0 0 Vernal T 0.2 0 0.2 0 0 0 0.4 0 0.4 U 1.4 0 1.4 0.6 0 0.6 0.2 0 0.2 Table 10. Statistical analysis of data from Table 9. Beaumont, TX. 2009. 8325 1.1 0 1.1 0 0 0 0 0 0 8360 1.5 0 1.5 0 0 0 0.2 0 0.2 HBK C5025 1.5 0 1.5 0 0 0 0.2 0 0.2 HBK C5941 1.8 0 1.8 0 0 0 0.1 0 0.1 HBK RR5123 1.6 0 1.6 0.2 0 0.2 0.1 0 0.1 HBK RR5425 1.2 0 1.2 0.1 0 0.1 0.2 0 0.2 S49 W6 RR 0.7 0 0.7 0 0 0 0.2 0 0.2 Vernal 0.8 0 0.8 0.3 0 0.3 0.3 0 0.3 Treated b 1.3 0 1.3 0.1 0 0.1 0.2 0 0.2 Untreated 1.3 0 1.3 0.1 0 0.1 0.1 0 0.1 P = 0.3530 --- 0.3530 0.5692 --- 0.5692 0.7827 --- 0.7827 Means in a column followed by the same or no letter are not significantly different (P = 0.05, ANOVA and LSD) 98
Table 11. Mean stink bug data (number per 15 sweeps) on Aug 3 for soybean host plant resistance. Beaumont, TX. 2009. 8325 T 0.4 0.4 0.8 0 0.4 0.4 0.2 0 0.2 U 0.8 0 0.8 0.4 0 0.4 0 0 0 8360 T 1.0 0.8 1.8 0.2 0 0.2 0 0 0 U 0.4 0 0.4 0 0 0 0.2 0 0.2 HBK C5025 T 0.8 0.4 1.2 0 0.4 0.4 0.2 0 0.2 U 1.0 1.8 2.8 0.2 0.2 0.4 0.6 0 0.6 HBK C5941 T 1.4 0.4 1.8 0.2 0 0.2 0.4 0 0.4 U 1.0 0.8 1.8 0 0.4 0.4 0 0 0 HBK RR5123 T 1.0 1.2 2.2 0.4 0.4 0.8 0.6 0 0.6 U 1.0 0.4 1.4 0.2 0 0.2 0.2 0 0.2 HBK RR5425 T 1.2 1.6 2.8 0 0.6 0.6 0.2 0 0.2 U 1.6 0.2 1.8 0 0 0 0 0 0 S49 W6 RR T 0.6 0.4 1.0 0 0 0 0 0 0 U 1.0 0.6 1.6 0 0.2 0.2 0.2 0 0.2 Vernal T 1.2 1.2 2.4 0 0.4 0.4 0 0 0 U 0.6 1.8 2.4 0.6 0 0.6 0.2 0 0.2 Table 12. Statistical analysis of data from Table 11. Beaumont, TX. 2009. 8325 0.6 0.2 0.8 0.2 0.2 0.4 0.1 0 0.1 8360 0.7 0.4 1.1 0.1 0 0.1 0.1 0 0.1 HBK C5025 0.9 1.1 2.0 0.1 0.3 0.4 0.4 0 0.4 HBK C5941 1.2 0.6 1.8 0.1 0.2 0.3 0.2 0 0.2 HBK RR5123 1.0 0.8 1.8 0.3 0.2 0.5 0.4 0 0.4 HBK RR5425 1.4 0.9 2.3 0 0.3 0.3 0.1 0 0.1 S49 W6 RR 0.8 0.5 1.3 0 0.1 0.1 0.1 0 0.1 Vernal 0.9 1.5 2.4 0.3 0.2 0.5 0.1 0 0.1 Treated b 1.0 0.8 1.8 0.1 0.3 0.4 0.2 0 0.2 Untreated 0.9 0.7 1.6 0.2 0.1 0.3 0.2 0 0.2 P = 0.9158 0.2389 0.7762 0.0757 0.2440 0.7306 0.2444 --- 0.2444 Means in a column followed by the same or no letter are not significantly different (P = 0.05, ANOVA and LSD) 99
Table 13. Mean stink bug data (number per 15 sweeps) on Sep 16 for soybean host plant resistance. Beaumont, TX. 2009. 8325 T 0.2 0.2 0.4 0 0 0 0.4 0 0.4 U 0.4 0.4 0.8 0 0 0 0.2 0.2 0.4 8360 T 0.2 0.4 0.6 0 0 0 0.2 0 0.2 U 0 0.4 0.4 0.4 0 0.4 0.2 0 0.2 HBK C5025 T 0 0 0 0.2 0.2 0.4 0.6 0.2 0.8 U 0.2 0.2 0.4 0.2 0 0.2 0 0.6 0.6 HBK C5941 T 0.4 1.0 1.4 0.2 0 0.2 0.4 0 0.4 U 0.6 1.0 1.6 0 0.2 0.2 0.6 0 0.6 HBK RR5123 T 0 0.4 0.4 0 0.2 0.2 0.4 0 0.4 U 0.2 0 0.2 0 0 0 0.8 0 0.8 HBK RR5425 T 0.4 0.2 0.6 0 0.2 0.2 0.2 0 0.2 U 0.6 0.2 0.8 0 0 0 0.8 0 0.8 S49 W6 RR T 0 0 0 0 0 0 0.6 0.2 0.8 U 0.2 0 0.2 0 0 0 0 0.2 0.2 Vernal T 0.6 0 0.6 0 0.2 0.2 0.4 0 0.4 U 0.4 0 0.4 0 0.2 0.2 0.6 0 0.6 Table 14. Statistical analysis of data from Table 13. Beaumont, TX. 2009. 8325 0.3 0.3 b 0.6 b 0 0 0 0.3 0.1 0.4 8360 0.1 0.4 b 0.5 b 0.2 0 0.2 0.2 0 0.2 HBK C5025 0.1 0.1 b 0.2 b 0.2 0.1 0.3 0.3 0.4 0.7 HBK C5941 0.5 1.0 a 1.5 a 0.1 0.1 0.2 0.5 0 0.5 HBK RR5123 0.1 0.2 b 0.3 b 0 0.1 0.1 0.6 0 0.6 HBK RR5425 0.5 0.2 b 0.7 b 0 0.1 0.1 0.5 0 0.5 S49 W6 RR 0.1 0 b 0.1 b 0 0 0 0.3 0.2 0.5 Vernal 0.5 0 b 0.5 b 0 0.2 0.2 0.5 0 0.5 Treated b 0.2 0.3 0.5 0.1 0.1 0.2 0.4 0.1 0.5 Untreated 0.3 0.3 0.6 0.1 0.1 0.1 0.4 0.1 0.5 P = 0.9052 0.8810 0.8977 0.6395 0.6983 0.7562 0.4653 0.9708 0.8140 Means in a column followed by the same or no letter are not significantly different (P = 0.05, ANOVA and LSD) 100