Endocytosis and Endocytic Network in Plants Communication in Plants: Neuronal Aspects of Plant Life Bonn, 29 November 2006
WHAT IS ENDOCYTOSIS? Endocytosis Endo (within) and Cytosis (cell) A process in which a substance gains entry into a cell without passing through the cell membrane Subdiving into three different types: 1. Receptor-mediated endocytosis 2. Pinocytosis 3. Phagocytosis
1. Receptor-mediated endocytosis 2. Pinocytosis 3. Phagocytosis
TYPES OF ENDOCYTOSIS Phagocytosis &
Signalling Endosomes Animal/Human Neurobiology Animal/Human Immunobiology McPherson P.S. et al. (2001) Signalling on the Endocytic Pathway. Traffic 2: 375-384 384 Sorkin A, Von Zastrow M (2002) Signal Transduction and Endocytosis: Close Encounters of Many Kinds. Nat Rev Mol Cell Biol 3: 600-614 614 Gonzalez-Gaitan Gaitan M. (2003) ) Signal Dispersal and Transduction Through the Endocytic Pathway. Nat Rev Mol Cell Biol 4: 213-224 224 Miaczynska M. et al. (2004) Not Just a Sink: Endosomes in Control of Signal Transduction. Curr Opin Cell Biol 16: 400-406 406 Le Borgne (2006) Regulation of Notch Signalling by Endocytosis and a Endosomal Sorting. Curr Opin Cell Biol 18: 1-101 10
Endosomes Control Trafficking and Localization of Receptors McPherson P.S. et al.. (Traffic( 2: 375-384 384, 2001)
Signalling Endosomes Between Neuronal Synapses and Nucleus McPherson P.S. et al.. (Traffic( 2: 375-384 384, 2001)
... IS THERE ANY ENDOCYTOSIS IN PLANTS? The 80 and 90 of the 20th Century: No The High Turgor Pressure Does Not Allow Any Endocytosis In n Plant Cells!!!
... IS THERE ANY ENDOCYTOSIS IN PLANTS? Baluška F, Šamaj J, Hlavačka ka A, Kendrick-Jones J, Volkmann D (2004) Myosin VIII and F-actin F enriched plasmodesmata in maize root inner cortex cells accomplish fluid-phase endocytosis via an actomyosin-dependent process. J Exp Bot 55: 463-473 473 Šamaj J, Baluška F, Voigt B, Schlicht M, Volkmann D, Menzel D (2004) 04) Endocytosis, actin cytoskeleton and signalling. Plant Physiol 135: 1150-116 11611 (Review) Voigt B, Timmers A, Šamaj J, Hlavacka A, Ueda T, Preuss M, Nielsen E, Mathur J, Emans N, Stenmark H, Nakano A, Baluška F, Menzel D (2005) Actin-based based motility of endosomes is linked to polar tip- growth of root hairs. Eur J Cell Biol 84, 609-621 621 Baluška F, Liners F, Hlavačka ka A, Schlicht M, Van Cutsem P, McCurdy D, Menzel D (2005) Cell wall pectins and xyloglucans are internalized into dividing root cells s and accumulate within cell plates during cytokinesis. Protoplasma 225: 141-155 155 Ovecka M, Lang I, Baluška F, Ismail A, Illeš P, Lichtscheidl IK (2005) Endocytosis and vesicle trafficking during tip growth of root hairs. Protoplasma 226: 39-54 Wang Q, Kong L, Li Y, Šamaj J, Baluška F, Lin J (2005) Effects of Brefeldin A on pollen germination and tube growth: antagonistic effects on endocytosis and secretion. Plant Physiol 139: 1692-1703 1703 Šamaj J, Read ND, Volkmann D, Menzel D, Baluška F (2005) The endocytic network in plants. Trends Cell Biol 15: 425-433 433 (Review) Šamaj J, Baluška F, F Menzel D (2005) Endocytosis in Plants. Springer Verlag (Book) Dhonukshe P, Baluška F, Schlicht M, Hlavačka ka A, Šamaj J, Friml J, Gadella Jr TWJ (2006) Endocytosis of cell surface material mediates cell plate formation during plant cytokinesis.. Dev Cell 10: 137-150 150 Baluška F, Menzel D, Barlow PW (2006) Cytokinesis in plant and animal cells: endosomes shut the door.. Dev Biol,, In press (Review)
Multivesicular Bodies (MVBs) Endosomes in Plants Winter and Hauser (Trends Plant Sci, In press)
Endocytosis: Plant Cell Nutrition Šamaj J, Baluška F, Menzel D (eds) Endocytosis in Plants (2005) Springer Verlag
Endocytosis in Plants 1. Plant Cell Nutrition 2. Cell Wall Remodelling 3. Tip Growth 4. Cytokinesis 5. Stomata Movements 6. Papilla Formation 7.. Innate Immunity 8.. Polar Auxin Transport 9.. Brassinosteroid Signalling 10.. Blue Light Perception Signalling 11.. Plasma Membrane Nucleus Signaling
Brefeldin A (BFA) Treatment Induces s BFA Compartments F-Actin-Dependent Endocytosis Drives Vesicle Recycling
BFA-Induced Compartment
Auxin Efflux Carrier (PIN1) Recycles At Cross-Walls Of Apical Cell Poles Of Root Cells B Control BFA PIN1 PIN1 A
BFA Compartments Cross Walls PIN2 PIN2 - BFA B Boonsirichai K et al - Plant Cell 15: 2612-2625 2625 (2003) A
Our bones are replaced via endocytosis... Bone-resorbing osteoclast Väänänen nen et al. (2000) what about plant cell walls?
Endocytosis: Cell Wall Remodelling Plant Physiol 130: 422-431 431 (2002) Plant Physiol 130: 4154 15-422 (2002)
RGII Pectins In Maize Root Cells BFA 2h
RGII Pectins In BFA-Treated Maize Root Cells
Endosomes - PPBs Microtubules Endosomes PPB Dhonukshe et al (Dev Cell 10: 137-150, 150, 2006)
Endosomes Cell Plate Golgi Stacks Endosomes Dhonukshe et al (Dev Cell 10: 137-150, 150, 2006)
PEN1 SNARE Papilla Formation
PEN1 SNARE Papilla Formation Assaad et al (Mol Biol Cell 15: 5118-5129 5129, 2004)
PEN1 SNARE Localizes to Endocytic BFA-induced Compartments and Cytokinetic Cell Plates PEN1-GFP line: Hans Thordal-Christensen (Boris Voigt)
PEN1 SNARE Localizes to Endocytic Clear Zone of Root Hairs PEN1 FM4-64 Control Cyt D PEN1-GFP line: Hans Thordal-Christensen (Boris Voigt)
FYVE-GFP Labelled Endosomes in Root Hairs Voigt et al (Eur J Cell Biol 84: 609-621, 621, 2005)
FYVE-GFP Labelled Endosomes in Root Hairs Accumulate at Outgrowing Bulges (b) and at Actively Growing Tips (c) Voigt et al (Eur J Cell Biol 84: 609-621, 621, 2005)
Ovecka et al (Protoplasma 226: 39-54, 2005) Endocytic Tracer FM1-43 in n Root Hairs
Plant Physiol 133: 1314-1321 1321 (2003) FM4-64
Fluid-Phase Endocytosis Of f Lucifer Yellow At t Plasmodesmata/Pit /Pit- Fields Of The Inner Cortex Cells PD CW PD CW Vacuole PD
Myosin VIII Actin Fluid-Phase Endocytosis Myosin VIII and Actin at Pit-Fields in Cells of the Inner Cortex Pit-Fields Pit-Fields Pit-Fields Pit-Fields Actin
Myosin VIII - Plasmodesmata Root Inner Cortex Cross-Section
Myosin VIII At Plasmodesmata/Pit-Fields In The Inner Cortex Young Cross- Wall CW Pit-Field Reichelt et al. (1999) Plant J 19: 555-567 567
Plasmodesmata Pit-Fields ields Plasmodesmata Plasmodesmata Grouped Into Pit-Field Pit-Field Evert et al. (1977) Planta 136: 77-89
Synapses Term was Coined in 1897 for Neurons by Charles Scott Sherrington Current Broader Definition: Asymmetric Actin-Based Adhesion Domains Specialised for Cell-to-Cell Communication Dustin ML, Colman DR (2002) Neural and immunological synaptic relations. Science 298: 785-789
Synapses A) Neuronal Synapses B) Immunological Synapses C) Virological Synapses D) Plant Synapses Baluška F, Volkmann D, Menzel D (2005) Plant synapses: actin-based adhesion domains for cell-to-cell communication. Trends Plant Sci 10: 106-111
Plant Synapses Endocytosis and Vesicle Recycling at Plant Synapses: From Auxin Transport to Signalling Baluška F et al (2003) Polar transport of auxin: Carrier-mediated flux across the plasma membrane or neurotransmitter-like secretion? Trends Cell Biol 13: 282-285 Baluška F, Volkmann D, Menzel D (2005) Plant synapses: actin-based adhesion domains for cell-to-cell communication. Trends Plant Sci 10: 106-111
Plant Synapses: Actin and Myosin Actin Myosin VIII Zea mays Baluška F, Barlow PW, Volkmann D (2000) Actin and myosin VIII in developing root cells. In: Actin: A Dynamic Framework For Multiple Plant Cell Functions, CJ Staiger, F Baluška, D Volkmann, PW Barlow (eds), Kluwer Academic Publishers, Dordrecht, The Netherlands, 457-476
Effects of Brefeldin A (BFA) on F-Actin and Plant Synapses Zea mays Control BFA 2h
Endosomal Plasma Membrane/Synapse Nucleus Signalling in Plants: Trithorax ATX1 ATX1-GFP Alvarez-Venegas R, Sadder M, Hlavacka A, Xia Y, Lu, G, Firsov A, Sarath G, Moriyama H, Baluška F, Avramova Z (2006) Phosphoinositide-5-phosphate phosphate and the Trithorax homolog, ATX1, define a novel regulatory pathway in Arabidopsis. Proc Natl Acad Sci USA, In press
Plant Synapses Blue Light Photoreceptor PHOTOTROPIN OTROPIN 1 Arabidopsis thaliana GFP-PHOT1 PHOT1 Green, FM4-64 Red Yinglang Wan Collaboration with Winslow Briggs
The blue light receptor photropin 1 (Phot1) is a flavin-binding light-activated protein kinase. The blue light-induces induces endocytosis of blue light receptor photropin 1, which emerges as part of the signal transduction pathway. The most rapid blue-light light induced endocytosis of photropin 1 was scored at root synapses.
Innate Immunnity Receptor Kinase FLS2 is Internalized in Response of Binding to Ligand Flagellin and Its Endocytosis is Needed for Its Signalling
Endosomes in Self/Non-Self Recognition Goldraij et al (Nature 439: 805-810, 810, 2006)