Two marks for correct bald answer 65 (%) Answer Acceptable answers Mark

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1(a) 13 (1) Two marks for correct bald answer 65 (%) 1(b) innate / instinctive / inherited Kinesis Ignore positive / negative Reject taxis (1) 1(c)(i) An explanation linking three of the following: the squirrels are marking their territory (1) Accept area for territory using chemical markers / chemical signals / pheromones / scent (1) which reduces conflict (1) reduces competition / results in more food for these squirrels / the offspring (1) Keep other squirrels away / out Ignore comments re scaring predators away / attracting mates / finding way home. 1(c)(ii) B habituation (1)

1(c)(iii) A description including three of the following: random / chance behaviour (1) Credit: operant behaviour in context of squirrels or other animals Trial and error behaviour (specific / repeated) behaviour rewarded (1) animal associates (reward with behaviour) (1) behaviour reinforced (1) (eventually) behaviour occurs without reward / infrequent reward / learned behaviour(1) Accept operant condition in terms of negative reinforcement / punishment (Total for question 1 = 10 marks)

2(a)(i) 90 780 = 0.115 (1) x 100 = 11.5(%) (1) Accept 12% 2(a)(ii) Any two from the following points respiration (1) excretion / egestion (1) temperature regulation (1) energy lost as heat movement / exercise not all eaten (1) 2(b) Any two from the following points: keep them in a warm environment (1) restrict their movement (1) provide {high energy / low wastage / easily digestible} food (1) Ignore feed more treat parasites (1) 2(c)(i) C (1)

2(c)(ii) An explanation linking the following points: bacteria provides nitrates for the plants (1) (by) nitrogen-fixation / converting nitrogen into nitrates (1) Accept nitrogen-fixing bacteria (nitrates) provide protein / for growth (1) (Total for question 2 = 10 marks)

3(a)(i) Answer B Mark (1) 3(a)(ii) Answer Mark An explanation that combines identification application of knowledge (1 mark) and reasoning/justification application of understanding (1 mark): the bacteria convert the ammonia into nitrites then nitrates maintaining the ph (1) (this prevents an increase in ph) which would cause enzymes to denature and kill the fish (1) 3(a)(iii) Answer Mark An explanation that combines identification understanding (1 mark) and reasoning/justification understanding (1 mark): the aquatic plant will take up nitrates by active transport (1) against the concentration gradient/from where there is a low concentration to where there is a high concentration of nitrates (1)

3(b) 3(c) Answer Additional guidance Marks An answer that combines the following points of application of knowledge and understanding to provide a logical description: a description of the use of a quadrat either by random sampling or using a belt transect (1) a sample size 10 100 and count the of clover plants in each quadrat (1) multiplication factor dependent on the of quadrats sampled (1) Answer to gain maximum marks steps must be in a logical sequence Mark An explanation that combines identification understanding (1 mark) and reasoning/justification understanding (3 marks): clover/leguminous plants could be used in crop rotation (1) where at intervals (2 3 years) a field is planted with clover/leguminous plants and left fallow (1) the clover/leguminous plants will have colonies of nitrogen fixing bacteria which will produce nitrates (1) the nitrates will increase the fertility of the soil and negate the need for artificial fertilisers (1) (4) (Total for question 3 = 12 marks)

4a(i) A autotrophically (1) 4a(ii) 7 760 / 97 000 = 0.08 (1) Award 3 marks for correct bald answer 0.08 x 100 = 8.00 (1) Accept alternate method of 100 8.00 = 92.00 (%) calculation OR 97000-7760 = 89240 (1) 89240/97000 = 0.92 (1) X 100 = 92(%) 4a(iii) Any two of the following: not all of the organisms are consumed (1) indigestible / egestion (1) excretion (1) movement (1) hunting / flying heat / respiration (1) reproduction (1)

Indicative Content QWC *4(b) An explanation to include some of the following points Mar k (6) mutualism involves organisms living closely with each other both organisms benefit oxpeckers relationship with large herbivores in Africa oxpecker feeds off of the parasitic insects that live on the herbivore disease reduced in herbivores from parasitic insect removal cleaner fish relationship with ocean species such as sharks and large fish cleaner fish eats the dead skin and parasites on the large fish or sharks large fish / sharks have disease reduced by removal of parasites nitrogen fixing bacteria relationship with leguminous plants such as beans bacteria live inside root nodules bacteria fix nitrogen for the plant to use bacteria obtain nutrition from the plant and are protected from the environment chemosynthetic bacteria relationship tubeworms in deep sea vents lack of light so no photosynthesis tubeworm gathers chemical substances needed by the bacteria for chemosynthesis / provide protection from heat bacteria produce chemicals for the tubeworm Level 0 No rewardable content 1 1-2 a limited explanation of at least one example of mutualism or definition of mutualism the answer communicates ideas using simple language and uses limited scientific terminology spelling, punctuation and grammar are used with limited accuracy 2 3-4 a simple explanation of at least two examples of mutualism or a detailed explanation of one the answer communicates ideas showing some evidence of clarity and organisation and uses scientific terminology appropriately spelling, punctuation and grammar are used with some accuracy 3 5-6 a detailed explanation of at least three examples of mutualism including nitrogen fixing bacteria or chemosynthetic bacteria the answer communicates ideas clearly and coherently uses a range of scientific terminology accurately spelling, punctuation and grammar are used with few errors Total for question 4 = 12 marks