Cell Culture Powder Media g Comment: Composition HS-No.: 3822 Storage: 2-8 C LGK: 10-13 S: 24/25 hygroscopic Powder media from AppliChem are mixed and processed according to the original formulations. To produce these media only raw materials of high quality are used. The single raw material that is used in the media production is taken only from one lot, which is dried according to instructions. Shortly, after drying the raw materials, they will be grinded in special mills to get a homogenous fine powder, that is packed immediately. Due to that the customer can easily dissolve our media. You will receive a weighing protocol on request. HEPES-buffered media HEPES-buffered media are often used, whenever the ph-value has to be stable in the media. With the addition of HEPES the ph value will get an additional stability in the ph range 7.2 to 7.6 for ph-sensitive cell culture systems. Deviations of the ph value in the media are caused by the metabolism of the cultivated cells but also by changes of the CO 2 concentration during incubation. Storage All powder media are produced without sodium hydrogen carbonate to increase the stability. Keeping them tightly closed is absolutely necessary as they are very hygroscopic. For this reason one package of powder media should always be dissolved completely. Storing dry and cold (+4 to +8 C) will increase the shelf life, which is normally two years. Generally the time for delivery will be one week. Customer formulations As a special service AppliChem offers the production of powder media according to the customer formulation for competitive prices - even in smaller quantities. In our laboratories we are able to consider most wishes regarding the composition of the powder media. The minimum order size is 20 liters with a delivery-date of two to three weeks, generally. Please ask for our offer for your formulation giving us the exact composition and the quantity you need. Literature ME: Eagle, H. (1965) Proc. Soc. Exp. iol. Med. 89, 362 DMEM: Dulbecco, R. & Freeman, G. (1959) Virology 8, 396 Smith et al. (1960) Virology 12, 185 DMEM/F12: Dulbecco, R. & Freeman, G. (1959) Virology 8, 396 Smith et al. (1960) Virology 12, 185 arnes, D. & Sato, G. (1980) Anal. iochem. 102, 255 Earle's buffered salt mixture: Earle, W. (1943) J. Nat. Cancer Inst.4, 165 Gamborg's 5 - Plant salts: Gamborg O.L., Miller R.A., OjimaK. (1968) Exp. Cell Res. 50,151 Glasgow - MEM (HK 21): MacPherson, I. & Stoker, M. (1961) Virology 16, 147 Grace's - Insect medium: Grace, T.D.C. (1962) Nature 195, 788 Grace, T.D.C. (1962) Nature216, 613 Grace, T.D.C. (1966) Nature 211, 366 Ham's F10 - Medium: Ham, R.G. (1963) Exp. Cell Res. 29, 515 Ham's F12K - Medium: Kaighn, M.E., (1973) Tissue Culture Methods and Applications, 54 (eds. Kruse & Patterson) Ham's F12 - Medium: Ham, R.G. (1965) Proc. Nat. Acad. Sci USA 53, 288 Hanks' buffered salt mixture: Hanks', J.H. & Wallace, R.E. (1949) Proc. Soc. Exp. iol. Med. 71, 196. Iscove's mod. Dulbecco's MEM (IMDM): Iscove, N., N. & Melchers, F (1978) J. Exp. Med. 147, 926 Iscove, N., N. et al. (1980) Exp. Cell Res. 126, 121 IPL-41 - Medium: Weiss, S.A., G.C. Smith, S.S. Kalter & J.L. Vaughn. (1981) In Vitro 17, 495. Joklik - MEM: Eagle, H. (1959) Science 130, 432 Leibovitz's L15 - Medium: Leibovitz, A. (1963) Amer. J. Hyg. 78, 173 Linsmaier, ednar & Skoog - Plant salts: Linsmaier E.M. & Skoog F. (1965) Physiol. Plantarum 18, 1 McCoy's 5A - Medium (modified): McCoy, T.A. et al. (1959) Proc. Soc. Exp. iol. Med. 1, 115 Hsu, T.C. & Kellogg, D.S. (1960) J. Nat. Cancer Inst.,25, 221 Iwakata, S. & Grace, J.T. (1964) N.Y. J. Med. 64, 2279 Medium 199: Morgan, J.F. et al. (1950) Proc. Soc. Exp. iol. Med. 73, 1 Morgan, J.F. et al. (1955) J. Nat. Cancer Inst. 16, 557 a-mem: Stanners, C.P. et al. (1971) Nature New iology 230, 52 Stanners, C.P. & Goldberg, V.J. (1975) J. Gen. Virol. 29, 281 MEM: Eagle, H. (1955) Science 122, 501 Eagle, H. (1959) Science 130, 432 S-MEM: Eagle, H. (1959) Science 130, 432 Murashige & Skoog - Plant medium: Murashige, T. & Skoog, F. (1962) Physiol. Plant. 15, 473 NCTC 135 - Medium: Evans, V.J. et al. (1964) Exp. Cell Res. 36, 439 PS - Powder: Dulbecco, R. & Vogt, M. (1957) J. Exp. Med. 106, 167 RPMI 1640 - Medium: Moore, G.E. et al. (1967) J.A.M.A. 199, 519 Schneider's Drosophila - Insect medium: Schneider, I. & lumenthal, A. (1978) iology and Genetics of Drosophila vol. 2A, Acad. Press, N.Y. pp. 266. TC-1 - Insect medium: Gardiner, G.R. & H. Stockdale (1975) J. Invert. Pathol. 25, 363 TNM-FH - Insect medium: Hink, W.F. (1970) Nature 266, 466 Hink, W.F. et al. (1974) In Vitro 9, 371 Waymouth's M 752/1 - Medium: Waymouth, C. (1959) J. Nat. Cancer Inst. 22, 13 William's Medium E: Williams, G.M. et al. (1971) Exp. Cell Res. 69, 106 Williams, G.M. & Gunn, J.M. (1974) Exp. Cell Res. 89, 139 Instructions for dissolving the powder to prepare liquid media Powdered media and salts are very hygroscopic and have to be stored under dry conditions. After opening one package you should dissolve the whole content at once. We recommend to produce 1X liquid medium from the powdered form, as the different amino acids may precipitate because of their low solubility. They are able to form salts which are of low solubility in concentrated solutions. If you have to add supplements to your medium, you can add them before filtration (unsterile) or after filtration (sterile). For dissolving the powdered medium please use bidistilled or deionized, pyrogen-free water. Procedure The required quantity of powdered medium will be completely dissolved under stirring in approx. 90 % of the water quantity. The temperature of the water should be between +15 and +30 C. The powder which remains in the package should be flushed out and dissolved with water. If the powder is completely dissolved, add the sodium hydrogen carbonate and dissolve it completely as well. The required quantities are listed in the table on the following pages. The ph (physiological optimum is ph 6.8-7.2) has to be adjusted with 1 N HCl or 1 N NaOH while stirring. The ph should be approx. 0.2 units lower than the target ph, because the ph will raise up a bit during filtration when the CO2 will leak out. After adjusting the ph you have to add water up to the desired volume and mix it very well and filter immediately. Store the medium at +4 C protected from light. 727
ME, asal medium with Earle's salts ME, asal medium with Earle's salts ME, asal medium with Earle's salts 10X ME, asal medium with Earle's salts 10X ME, asal medium with Earle's salts ME, asal medium with Earle's salts (diploide) ME, asal medium with Earle's salts (diploide) ME, asal medium with Earle's salts, autoclavable ME, asal medium with Earle's salts ME, asal medium with Earle's salts A1310,10 1L A1310,50 5L A1310,9010 10 L A1310,9050 50 L A1311,10 1L A1311,50 5L A1311,9010 10 L A1311,9050 50 L A1977,10 1L A1977,50 5L A1977,9010 10 L A1977,9050 50 L A1983,10 1L A1983,50 5L A1983,9010 10 L A1983,9050 50 L A1984,10 1L A1984,50 5L A1984,9010 10 L A1984,9050 50 L A1312,10 1L A1312,50 5L A1312,9010 10 L A1312,9050 50 L A1976,10 1L A1976,50 5L A1976,9010 10 L A1976,9050 50 L A1915,10 1L A1915,50 5L A1915,9010 10 L A1915,9050 50 L A1916,10 1L A1916,50 5L A1916,9010 10 L A1916,9050 50 L 728
ME, asal medium with Hanks' salts ME, asal medium with Hanks' salts ME, asal medium with Hanks' salts 10X ME, asal medium with Hanks' salts 10X ME, asal medium with Hanks' salts ME, asal medium with Hanks' salts, autoclavable ME, asal medium with Hanks' salts ME, asal medium with Hanks' salts DMEM (Dulbecco's mod. Eagle - Media) A1917,10 1L A1917,50 5L A1917,9010 10 L A1917,9050 50 L A1313,10 1L A1313,50 5L A1313,9010 10 L A1313,9050 50 L A1990,10 1L A1990,50 5L A1990,9010 10 L A1990,9050 50 L A1988,10 1L A1988,50 5L A1988,9010 10 L A1988,9050 50 L A1978,10 1L A1978,50 5L A1978,9010 10 L A1978,9050 50 L A1918,10 1L A1918,50 5L A1918,9010 10 L A1918,9050 50 L A1919,10 1L A1919,50 5L A1919,9010 10 L A1919,9050 50 L DMEM (Dulbecco's mod. Eagle - Medium) with 1.0 g/l D(+)-Glucose with Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 1.0 g/l D(+)-Glucose with Sodium pyruvate A1924,10 1L A1924,50 5L A1924,9010 10 L A1924,9050 50 L A1314,10 1L A1314,50 5L A1314,9010 10 L A1314,9050 50 L 729
DMEM (Dulbecco's mod. Eagle - Medium) with 1.0 g/l D(+)-Glucose with Sodium pyruvate without Phenol red DMEM (Dulbecco's mod. Eagle - Medium) with 1.0 g/l D(+)-Glucose with Sodium pyruvate without Phenol red DMEM (Dulbecco's mod. Eagle - Medium) with 1.0 g/l D(+)-Glucose with Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 1.0 g/l D(+)-Glucose with Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose without Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose without Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose with Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose with Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose with Sodium pyruvate without Phenol red DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose with Sodium pyruvate without Phenol red A0826,10 1L A0826,50 5L A0826,9010 10 L A0826,9050 50 L A3134,10 1L A3134,50 5L A3134,9010 10 L A3134,9050 50 L A1925,10 1L A1925,50 5L A1925,9010 10 L A1925,9050 50 L A1315,10 1L A1315,50 5L A1315,9010 10 L A1315,9050 50 L A1926,10 1L A1926,50 5L A1926,9010 10 L A1926,9050 50 L A1316,10 1L A1316,50 5L A1316,9010 10 L A1316,9050 50 L A1985,10 1L A1985,50 5L A1985,9010 10 L A1985,9050 50 L A1986,10 1L A1986,50 5L A1986,9010 10 L A1986,9050 50 L A3137,10 1L A3137,50 5L A3137,9010 10 L A3137,9050 50 L A3139,10 1L A3139,50 5L A3139,9010 10 L A3139,9050 50 L 730
DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose without Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose without Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose with Sodium pyruvate DMEM (Dulbecco's mod. Eagle - Medium) with 4.5 g/l D(+)-Glucose with Sodium pyruvate DMEM/F12 (1:1) - Media A1950,10 1L A1950,50 5L A1950,9010 10 L A1950,9050 50 L A1965,10 1L A1965,50 5L A1965,9010 10 L A1965,9050 50 L A1979,10 1L A1979,50 5L A1979,9010 10 L A1979,9050 50 L A1951,10 1L A1951,50 5L A1951,9010 10 L A1951,9050 50 L DMEM/F12 (1:1) - Medium DMEM/F12 (1:1) - Medium DMEM/F12 (1:1) - Medium with 15 mm HEPES DMEM/F12 (1:1) - Medium with 15 mm HEPES DMEM/F12 (1:1) - Medium DMEM/F12 (1:1) - Medium A1920,10 1L A1920,50 5L A1920,9010 10 L A1920,9050 50 L A1318,10 1L A1318,50 5L A1318,9010 10 L A1318,9050 50 L A1922,10 1L A1922,50 5L A1922,9010 10 L A1922,9050 50 L A1317,10 1L A1317,50 5L A1317,9010 10 L A1317,9050 50 L A1923,10 1L A1923,50 5L A1923,9010 10 L A1923,9050 50 L A1921,10 1L A1921,50 5L A1921,9010N 10 L A1921,9050 50 L 731
Earle's balanced salts (ESS) Earle's balanced salts (ESS) with 1.0 g/l D(+)-Glucose with Phenol red Earle's balanced salts (ESS) with 1.0 g/l D(+)-Glucose without Phenol red Earle's balanced salts (ESS) with 1.0 g/l D(+)-Glucose with Phenol red without Calcium and Magnesium Earle's balanced salts (ESS) with 1.0 g/l D(+)-Glucose without Calcium and Magnesium without Phenol red Gamborg's 5 Plant salts A1712,50 5L A1712,9010 10 L A1712,9050 50 L A2039,50 5L A2039,9010 10 L A2039,9050 50 L A3026,50 5L A3026,9010 10 L A3026,9050 50 L A3127,50 5L A3127,9010 10 L A3127,9050 50 L A1911,50 5L A1911,9010 10 L A1911,9050 50 L Glasgow - MEM (HK 21) Glasgow - MEM (HK 21) without Tryptose/Phosphate-ouillon Glasgow - MEM (HK 21) without Tryptose/Phosphate-ouillon Glasgow - MEM (HK 21) with Tryptose/Phosphate-ouillon Glasgow - MEM (HK 21) with Tryptose/Phosphate-ouillon A2024,10 1L A2024,50 5L A2024,9010 10 L A2024,9050 50 L A1320,10 1L A1320,50 5L A1320,9010 10 L A1320,9050 50 L A2025,10 1L A2025,50 5L A2025,9010 10 L A2025,9050 50 l A1321,10 1L A1321,50 5L A1321,9010 10 L A1321,9050 50 L 732
Grace's - Insect medium Grace's - Insect medium Grace's - Insect medium Ham's F10 - Medium A2023,10 1L A2023,50 5L A2023,9010 10 L A2023,9050 50 L A1529,10 1L A1529,50 5L A1529,9010 10 L A1529,9050 50 L Ham's F10 - Medium Ham's F10 - Medium Ham's F10 - Medium Ham's F10 - Medium Ham's F10 - Medium with 1.2 g/l Sodium hydrogen carbonate Ham's F10 - Medium with 1.2 g/l Sodium hydrogen carbonate Ham's F12 - Medium A1970,10 1L A1970,50 5L A1970,9010 10 L A1970,9050 50 L A1525,10 1L A1525,50 5L A1525,9010 10 L A1525,9050 50 L A1989,10 1L A1989,50 5L A1989,9010 10 L A1989,9050 50 L A1982,10 1L A1982,50 5L A1982,9010 10 L A1982,9050 50 L A2026,10 1L A2026,50 5L A2026,9010 10 L A2026,9050 50 L A1952,10N 1L A1952,50 5L A1952,9010 10 L A1952,9050 50 L Ham's F12 - Medium A1948,10 1L A1948,50 5L A1948,9010 10 L A1948,9050 50 L 733
Ham's F12 - Medium Ham's F12 - Medium Ham's F12 - Medium Ham's F12K - Medium A1526,10 1L A1526,50 5L A1526,9010 10 L A1526,9050 50 L A1969,10 1L A1969,50 5L A1969,9010 10 L A1969,9050 50 L A1953,10 1L A1953,50 5L A1953,9010 10 L A1953,9050 50 L Ham's F12K - Medium Ham's F12K - Medium Hanks' balanced salts (HSS) A1963,10 1L A1963,50 5L A1963,9010 10 L A1963,9050 50 L A1927,10 1L A1927,50 5L A1927,9010 10 L A1927,9050 50 L Hanks' balanced salts (HSS) with 1.0 g/l D(+)-Glucose with Phenol red Hanks' balanced salts (HSS) with 1.0 g/l D(+)-Glucose without Phenol red Hanks' balanced salts (HSS) with 1.0 g/l D(+)-Glucose without Calcium and Magnesium with Phenol red Hanks' balanced salts (HSS) with 1.0 g/l D(+)-Glucose without Calcium and Magnesium without Phenol red HEPES - balanced salts A1711,50 5L A1711,9010 10 L A1711,9050 50 L A3140,50 5L A3140,9010 10 L A3140,9050 50 L A2729,50 5L A2729,9010 10 L A2729,9050 50 L A2512,50 5L A2512,9010 10 L A2512,9050 50 L A0629,10 1L A0629,50 5L A0629,9010 10 L 734
IPL 41 - Medium IPL 41 - Medium IPL 41 - Medium Iscove's mod. Dulbecco's - Medium (IMDM) A2027,10 1L A2027,50 5L A2027,9010 10 L A2027,9050 50 L A1964,10 1L A1964,50 5L A1964,9010 10 L A1964,9050 50 L Iscove's mod. Dulbecco's - Medium (IMDM) Iscove's mod. Dulbecco's - Medium (IMDM) Iscove's mod. Dulbecco's - Medium (IMDM) Iscove's mod. Dulbecco's - Medium (IMDM) Joklik - MEM, modified for spinner culture A2028,10 1L A2028,50 5L A2028,9010 10 L A2028,9050 50 L A1909,10 1L A1909,50 5L A1909,9010 10 L A1909,9050 50 L A2029,10 1L A2029,50 5L A2029,9010 10 L A2029,9050 50 L A1322,10 1L A1322,50 5L A1322,9010 10 L A1322,9050 50 L Joklik - MEM, modified for spinner culture with Earle's salt (modified) without Antibiotics without Calcium chloride Joklik - MEM, modified for spinner culture with Earle's salts (modified) without Antibiotics without Calcium chloride A1974,10 1L A1974,50 5L A1974,9010 10 L A1974,9050 50 L A1539,10 1L A1539,50 5L A1539,9010 10 L A1539,9050 50 L 735
Leibovitz's L15 - Medium Leibovitz's L15 - Medium Leibovitz's L15 - Medium Leibovitz's L15 - Medium Leibovitz's L15 - Medium Linsmaier, ednar & Skoog Plant salts A1967,10 1L A1967,50 5L A1967,9010 10 L A1967,9050 50 L A1323,10 1L A1323,50 5L A1323,9010 10 L A1323,9050 50 L A1968,10 1L A1968,50 5L A1968,9010 10 L A1968,9050 50 L A1954,10 1L A1954,50 5L A1954,9010 10 L A1954,9050 50 L A0591,50 5L A0591,9010 10 L A0591,9050 50 L McCoy's 5A - Medium (modified) McCoy's 5A - Medium (modified) without Serum McCoy's 5A - Medium (modified) without Serum McCoy's 5A - Medium (modified) without Serum McCoy's 5A - Medium (modified) without Serum A2034,10 1L A2034,50 5L A2034,9010 10 L A2034,9050 50 L A1324,10 1L A1324,50 5L A1324,9010 10 L A1324,9050 50 L A2035,10 1L A2035,50 5L A2035,9010 10 L A2035,9050 50 L A1955,10 1L A1955,50 5L A1955,9010 10 L A1955,9050 50 L 736
Medium 199 with Earle's Salts Medium 199 with Earle's Salts Medium 199 with Earle's Salts Medium 199 with Earle's Salts Medium 199 with Earle's Salts Medium 199 with Hanks' Salts A2022,10 1L A2022,50N 5L A2022,9010 10 L A2022,9050 50 L A1325,10 1L A1325,50 5L A1325,9010 10 L A1325,9050 50 L A2030,10 1L A2030,50 5L A2030,9010 10 L A2030,9050 50 L A1326,10 1L A1326,50 5L A1326,9010 10 L A1326,9050 50 L Medium 199 with Hanks' Salts Medium 199 with Hanks' Salts Medium 199 with Hanks' Salts Medium 199 with Hanks' Salts a-mem A2031,10 1L A2031,50 5L A2031,9010 10 L A2031,9050 50 L A1327,10 1L A1327,50 5L A1327,9010 10 L A1327,9050 50 L A2021,10 1L A2021,50 5L A2021,9010 10 L A2021,9050 50 L A1328,10 1L A1328,50 5L A1328,9010 10 L A1328,9050 50 L a-mem without Deoxyribonucleosides without Ribonucleosides A1971,10 1L A1971,50 5L A1971,9010 10 L A1971,9050 50 L 737
a-mem without Deoxyribonucleosides without Ribonucleosides a-mem with Ribonucleosides with Deoxyribonucleosides a-mem with Deoxyribonucleosides with Ribonucleosides a-mem without Deoxyribonucleosides without Ribonucleosides a-mem without Deoxyribonucleosides without Ribonucleosides a-mem with Deoxyribonucleosides with Ribonucleosides a-mem with Desoxyribonucleosides with Ribonucleosides MEM with Earle's salts A1330,10 1L A1330,50 5L A1330,9010 10 L A1330,9050 50 L A1913,10 1L A1913,50 5L A1913,9010 10 L A1913,9050 50 L A1329,10 1L A1329,50 5L A1329,9010 10 L A1329,9050 50 L A1980,10 1L A1980,50 5L A1980,9010 10 L A1980,9050 50 L A1972,10 1L A1972,50 5L A1972,9010 10 L A1972,9050 50 L A1949,10 1L A1949,50 5L A1949,9010 10 L A1949,9050 50 L A1914,10 1L A1914,50 5L A1914,9010 10 L A1914,9050 50 L MEM with Earle's salts MEM with Earle's salts MEM with Earle's salts, autoclavable A1331,10 1L A1331,50 5L A1331,9010 10 L A1331,9050 50 L A1332,10 1L A1332,50 5L A1332,9010 10 L A1332,9050 50 L A2597,10 1L A2597,50 5L A2597,9010 10 L A2597,9050 50 L 738
MEM with Earle's salts with NEAA MEM with Earle's salts with NEAA MEM with Earle's salts MEM with Earle's salts MEM with Earle's salts with NEAA MEM with Earle's salts with NEAA MEM with Hanks' salts A2018,10N 1L A2018,50 5L A2018,9010N 10 L A2018,9050 50 L A1334,10 1L A1334,50 5L A1334,9010 10 L A1334,9050 50 L A2019,10 1L A2019,50 5L A2019,9010 10 L A2019,9050 50 L A1333,10 1L A1333,50 5L A1333,9010 10 L A1333,9050 50 L A2032,10 1L A2032,50 5L A2032,9010 10 L A2032,9050 50 L A1956,10 1L A1956,50 5L A1956,9010 10 L A1956,9050 50 L MEM with Hanks' salts MEM with Hanks' salts MEM with Hanks' salts with NEAA MEM with Hanks' salts with NEAA A1957,10 1L A1957,50 5L A1957,9010 10 L A1957,9050 50 L A1335,10 1L A1335,50 5L A1335,9010 10 L A1335,9050 50 L A2041,10 1L A2041,50 5L A2041,9010 10 L A2041,9050 50 L A1337,10 1L A1337,50 5L A1337,9010 10 L A1337,9050 50 L 739
MEM with Hanks'salts MEM with Hanks' salts MEM with Hanks' salts with NEAA MEM with Hanks' salts with NEAA S-MEM, modified for spinner culture A2042,10 1L A2042,50 5L A2042,9010 10 L A2042,9050 50 L A1336,10 1L A1336,50 5L A1336,9010 10 L A1336,9050 50 L A2043,10 1L A2043,50 5L A2043,9010 10 L A2043,9050 50 L A1958,10 1L A1958,50 5L A1958,9010 10 L A1958,9050 50 L S-MEM, modified for spinner culture with Earle's salt (modified) S-MEM, modified for spinner culture with Earle's salts (modified) S-MEM, modified for spinner culture, autoclavable with Earle's salt (modified) autoclavable NCTC 135 - Medium A1338,10 1L A1338,50 5L A1338,9010 10 L A1338,9050 50 L A1308,10 1L A1308,50 5L A1308,9010 10 L A1308,9050 50 L A1975,10 1L A1975,50 5L A1975,9010 10 L A1975,9050 50 L NCTC 135 - Medium NCTC 135 - Medium A2020,10 1L A2020,50 5L A2020,9010 10 L A2020,9050 50 L A1528,10 1L A1528,50 5L A1528,9010 10 L A1528,9050 50 L 740
PS buffer (Dulbecco's) - Powder Phosphate buffered saline, Phosphate buffered saline, 10X concentrated PS buffer (1X, Dulbecco's) - Powder PS buffer (10X Dulbecco's) - Powder PS buffer (1X Dulbecco's) - Powder with 0,01 % Thimerosal with 0,02 % EDTA RPMI 1640 - Medium A0964,9010 10 L A0964,9050 50 L A0964,91 1 L A0965,9010 10 L A0965,9050 50 L A0965,91 1 L A1908,9010 10 L A1908,9050 50 L A1908,91 1 L RPMI 1640 - Medium RPMI 1640 - Medium RPMI 1640 - Medium without Phenol red RPMI 1640 - Medium without Phenol red RPMI 1640 - Medium RPMI 1640 - Medium A1959,10 1L A1959,50 5L A1959,9010 10 L A1959,9050 50 L A1339,10 1L A1339,50 5L A1339,9010 10 L A1339,9050 50 L A2040,10 1L A2040,50 5L A2040,9010 10 L A2040,9050 50 L A3135,10 1L A3135,50 5L A3135,9010 10 L A3135,9050 50 L A1960,10 1L A1960,50 5L A1960,9010 10 L A1960,9050 50 L A1538,10 1L A1538,50 5L A1538,9010 10 L A1538,9050 50 L 741
Click - RPMI - Medium Click - RPMI - Medium Click - RPMI - Medium Schneider's Drosophila - Insect medium A2044,10 1L A2044,50 5L A2044,9010 10 L A2044,9050 50 L A2504,10 1L A2504,50 5L A2504,9010 10 L A2504,9050 50 L Schneider's Drosophila - Insect medium Schneider's Drosophila - Insect medium TC 1 - Insect medium A2036,10N 1L A2036,50 5L A2036,9010N 10 L A2036,9050 50 L A1533,10 1L A1533,50 5L A1533,9010 10 L A1533,9050 50 L TC 1 - Insect medium A2033,10 1L A2033,50 5L A2033,9010 10 L A2033,9050 50 L TC 1 - Insect medium TNM-FH - Insect medium (modifiziertes Grace's Insektenmedium) A2017,10 1L A2017,50 5L A2017,9010 10 L A2017,9050 50 L modified Grace's Insect medium TNM-FH - Insect medium (modified Grace's Insect medium) with Lactalbumin hydrolysate with Yeast extracts TNM-FH - Insect medium (modified Grace's Insect medium) with Lactalbumin hydrolysate with Yeast extracts A2037,10 1L A2037,50 5L A2037,9010 10 L A2037,9050 50 L A1570,10 1L A1570,50 5L A1570,9010 10 L A1570,9050 50 L 742
Waymouth's M 752/1 - Medium Waymouth's M 752/1 - Medium Waymouth's M 752/1 - Medium Waymouth's M 752/1 - Medium with 20 mm HEPES Waymouth's M 752/1 - Medium with 20 mm HEPES William's Media E A1973,10 1L A1973,50 5L A1973,9010N 10 L A1973,9050 50 L A1527,10 1L A1527,50 5L A1527,9010 10 L A1527,9050 50 L A1981,10 1L A1981,50 5L A1981,9010 10 L A1981,9050 50 L A1961,10 1L A1961,50 5L A1961,9010 10 L A1961,9050 50 L William's Medium E William's Medium E William's Medium E without Phenol red William's Medium E without Phenol red William's Medium E A1309,10 1L A1309,50 5L A1309,9010 10 L A1309,9050 50 L A1910,10 1L A1910,50 5L A1910,9010 10 L A1910,9050 50 L A3029,10 1L A3029,50 5L A3029,9010 10 L A3029,9050 50 L A3136,10 1L A3136,50 5L A3136,9010 10 L A3136,9050 50 L A1966,10N 1L A1966,50 5L A1966,9010 10 L A1966,9050 50 L 743
William's Medium E A1962,10 1L A1962,50 5L A1962,9010 10 L A1962,9050 50 L 744
Table for the addition of sodium hydrogen carbonate Description Article-No. NaHCO3 (g / L) ME, asal medium with Earle's salts A1310 2.2 ME, asal medium with Earle's salts A1311 2.2 10X ME, asal medium with Earle's salts A1977 2.2 / L 1X 10X ME, asal medium with Earle's salts A1983 2.2 / L 1X ME, asal medium with Earle's salts, with HEPES A1915 2.2 ME, asal medium with Earle's salts, with HEPES A1916 2.2 ME, asal medium with Earle's (diploide) A1984 2.2 ME, asal medium with Earle's (diploide) A1312 2.2 ME, asal medium with Hanks' salts A1917 0.350 ME, asal medium with Hanks' salts A1313 0.350 10X ME, asal medium with Hanks' salts A1990 0.350 / L 1X 10X ME, asal medium with Hanks' salts A1988 0.350 / L 1X ME, asal medium with Hanks' salts; autoclavable A1976 30 ml 7.5 % - Solution / L after autoclaving ME, asal medium with Hanks' salts; autoclavable A1978 5 ml 7.5 % - Solution / L after autoclaving ME, asal medium with Hanks' salts, with HEPES A1918 0.350 ME, asal medium with Hanks' salts, with HEPES A1919 0.350 DMEM - Medium A1924 3.7 DMEM - Medium A1314 3.7 DMEM - Medium with HEPES A1925 0.850 DMEM - Medium with HEPES A1315 0.850 DMEM - Medium A1926 3.7 DMEM - Medium A1316 3.7 DMEM - Medium A1985 3.7 DMEM - Medium A1986 3.7 DMEM - Medium with HEPES A1950 0.850 DMEM - Medium with HEPES A1965 0.850 DMEM - Medium with HEPES A1979 0.850 DMEM - Medium with HEPES A1951 0.850 DMEM - Medium A0826 3.7 DMEM - Medium A3134 3.7 DMEM - Medium A3137 3.7 DMEM - Medium A3139 3.7 DMEM/F12 (1:1) - Medium A1920 1.2 DMEM/F12 (1:1) - Medium A1318 1.2 DMEM/F12 (1:1) - Medium with 15 mm HEPES A1922 1.2 DMEM/F12 (1:1) - Medium with 15 mm HEPES A1317 1.2 DMEM/F12 (1:1) - Medium A1923 1.2 DMEM/F12 (1:1) - Medium A1921 1.2 Earle's balanced salts A1712 2.2 Earle's balanced salts A2039 2.2 Earle's balanced salts A3026 2.2 Earle's balanced salts A3127 2.2 Gamborg's 5 - Plant salts A1911 none Glasgow - MEM (HK 21) A2024 2.750 Glasgow - MEM (HK 21) A1320 2.750 Glasgow - MEM (HK 21) A2025 2.750 Glasgow - MEM (HK 21) A1321 2.750 Grace's - Insect medium A2023 0.350 Grace's - Insect medium A1529 0.350 Ham's F10 - Medium A1970 1.2 Ham's F10 - Medium A1525 1.2 Ham's F10 - Medium with HEPES A1989 1.2 Ham's F10 - Medium with HEPES A1982 1.2 Ham's F10 - Medium with HEPES A1952 1.2 Ham's F10 - Medium with HEPES A2026 1.2 Ham's F12K - Medium A1963 2.5 Ham's F12K - Medium A1927 2.5 Ham's F12 - Medium A1948 1.176 Ham's F12 - Medium A1526 1.176 Ham's F12 - Medium with HEPES A1969 1.176 Ham's F12 - Medium with HEPES A1953 1.176 Ham's F12K - Medium A1963 2.5 Ham's F12K - Medium A1927 2.5 Hanks' balanced salts A1711 0.350 Hanks' balanced salts A3140 0.350 745
Table for the addition of sodium hydrogen carbonate Description Article-No. NaHCO3 (g / L) Hanks' balanced salts A2729 0.350 Hanks' balanced salts A2512 0.350 HEPES balanced salts A0629 none Iscove's mod. Dulbecco's - Medium (IMDM) A2028 3.7 Iscove's mod. Dulbecco's - Medium (IMDM) A1909 3.7 Iscove's mod. Dulbecco's - Medium (IMDM) A2029 3.024 Iscove's mod. Dulbecco's - Medium (IMDM) A1322 3.024 IPL 41 - Medium A2027 0.350 IPL 41 - Medium A1964 0.350 Joklik - MEM, modified for spinner culture A1974 2.0 Joklik - MEM, modified for spinner culture A1539 2.0 Leibovitz's L-15 - Medium A1967 none Leibovitz's L-15 - Medium A1323 none Leibovitz's L-15 - Medium with HEPES A1968 none Leibovitz's L-15 - Medium with HEPES A1954 none Linsmaier, ednar & Skoog Plant salts A0591 none McCoy's 5A - Medium (modified) A2034 2.2 McCoy's 5A - Medium (modified) A1324 2.2 McCoy's 5A - Medium (modified) A2035 2.2 McCoy's 5A - Medium (modified) A1955 2.2 Medium 199 with Earle's salts A2022 2.2 Medium 199 with Earle's salts A1325 2.2 Medium 199 with Earle's salts, with HEPES A2030 0.850 Medium 199 with Earle's salts, with HEPES A1326 0.850 Medium 199 with Hanks' salts A2031 0.350 Medium 199 with Hanks' salts A1327 0.350 Medium 199 with Hanks' salts, with HEPES A2021 0.350 Medium 199 with Hanks' salts, with HEPES A1328 0.350 a - MEM A1971 2.2 a - MEM A1330 2.2 a - MEM A1913 2.2 a - MEM A1329 2.2 a - MEM with HEPES A1980 0.850 a - MEM with HEPES A1972 0.850 a - MEM with HEPES A1949 0.850 a - MEM with HEPES A1914 0.850 MEM with Earle's salts A1331 2.2 MEM with Earle's salts A1332 2.2 MEM with Earle's salts A2018 2.2 MEM with Earle's salts A1334 2.2 MEM with Earle's salts, with HEPES A2019 0.850 MEM with Earle's salts, with HEPES A1333 0.850 MEM with Earle's salts, with HEPES A2032 0.850 MEM with Earle's salts, with HEPES A1956 0.850 MEM with Earle's salts; autoclavable A2597 30 ml 7.5 % - Solution / L after autoclaving MEM with Hanks' salts A1957 0.350 MEM with Hanks' salts A1335 0.350 MEM with Hanks' salts A2041 0.350 MEM with Hanks' salts A1337 0.350 MEM with Hanks' salts, with HEPES A2042 0.350 MEM with Hanks' salts, with HEPES A1336 0.350 MEM with Hanks' salts, with HEPES A2043 0.350 MEM with Hanks' salts, with HEPES A1958 0.350 S-MEM, modified for spinner culture A1338 2.2 S-MEM, modified for spinner culture A1308 2.2 S-MEM, modified for spinner culture; autoclavable A1975 30 ml 7.5 % - Solution / L after autoclaving Murashige & Skoog - Plant salts A1534 none Murashige & Skoog - Plant salts A1535 none NCTC 135 - Medium A2020 2.2 NCTC 135 - Medium A1528 2.2 PS buffer (1X, Dulbecco) - Powder A0964 none PS buffer (10X, Dulbecco) - Powder A0965 none PS buffer (1X, Dulbecco) - Powder A1908 none RPMI 1640 A1959 2.2 RPMI 1640 A1339 2.2 746
Table for the addition of sodium hydrogen carbonate Description Article-No. NaHCO3 (g / L) RPMI 1640 with HEPES A1960 0.850 RPMI 1640 with HEPES A1538 0.850 RPMI 1640 A2040 2.0 RPMI 1640 A3135 2.0 Click-RPMI A2044 1.175 Click-RPMI A2504 1.175 Schneider's Drosophila - Insect medium A2036 0.4 Schneider's Drosophila - Insect medium A1533 0.4 TC-1 - Medium A2033 0.350 TC-1 - Medium A2017 0.350 TNM-FH - Insect medium A2037 0.350 TNM-FH - Insect medium A1570 0.350 Tris - Citrate balanced salts A0692 none Waymouth's M 752/1 - Medium A1973 2.240 Waymouth's M 752/1 - Medium A1527 2.240 Waymouth's M 752/1 - Medium with 20 mm HEPES A1981 0.850 Waymouth's M 752/1 - Medium with 20 mm HEPES A1961 0.850 William's Medium E A1309 2.2 William's Medium E A1910 2.2 William's Medium E with HEPES A1966 0.850 William's Medium E with HEPES A1962 0.850 William's Medium E A3029 2.2 William's Medium E A3136 2.2 747
ME, asal medium with Earle's salts Inorganic salts Other Components Components A1310 A1311 A1977 A1983 A1915 A1916 A1984 A1312 A1976 autoclavable Calcium chloride 264.92 264.92 2649.20 2649.20 264.92 264.92 264.92 264.92 264.92 x2h 2O Magnesium 93.68 93.68 chloride anhydrous Magnesium 139,52 139,52 1395,20 1395,20 139,52 139,52 139,52 sulfate dried Potassium 4. 4. 40. 40. 4. 4. 4. 4. 4. chloride Sodium chloride 68. 68. 680. 680. 63. 63. 68. 68. 68. Sodium dihydrogen phosphate x H2O 140. 140. 14. 14. 140. 140. 140. 140. 140. D(+)-Glucose 10. 10. 1. 1. 10. 10. 10. 10. 10. anhydrous HEPES 5958. 5958. Phenol red 10. 10. 1. 1. 10. 10. 10. 10. 6. Succinic acid 75. Succinic acid sodium salt 1. Amino acids L-Arginine x HCl 21. 21. 210. 210. 21. 21. 21. 21. 21. L-Cystine 12. 12. 120. 120. 12. 12. 12. 12. 12. L-Glutamine 0 292. 0 2920. 0 292. 0 292. 0 L-Histidine ase 8. 8. 80. 80. 8. 8. 8. 8. L-Histidine x HCl 10.81 10.81 15. xh2o L-Isoleucine 26. 26. 260. 260. 26. 26. 26. 26. 26. L-Leucine 26. 26. 260. 260. 26. 26. 26. 26. 26. L-Lysine x HCl 36.47 36.47 364.70 364.70 36.47 36.47 36.47 36.47 36.47 L-Methionine 7.50 7.50 75. 75. 7.50 7.50 7.50 7.50 7.50 L-Phenylalanine 16.50 16.50 165. 165. 16.50 16.50 16.50 16.50 16.50 L-Threonine 24. 24. 240. 240. 24. 24. 24. 24. 24. L-Tryptophan 4. 4. 40. 40. 4. 4. 4. 4. 4. L-Tyrosine 18. 18. 180. 180. 18. 18. 18. 18. 18. L-Valine 23.50 23.50 235. 235. 23.50 23.50 23.50 23.50 23.50 Vitamins D(+)-iotin 1. 1. 10. 10. 1. 1. 1. 1. 1. D-Calcium 1. 1. 10. 10. 1. 1. 1. 1. 1. pantothenate Choline bitartrate 1.80 Choline chloride 1. 1. 10. 10. 1. 1. 1. 1. Folic acid 1. 1. 10. 10. 1. 1. 1. 1. 1. myo-inositol 2. 2. 20. 20. 2. 2. 2. 2. 2. Nicotinamide 1. 1. 10. 10. 1. 1. 1. 1. 1. Pyridoxal x HCl 1. 1. 10. 10. 1. 1. 1. 1. 1. Riboflavin 0.10 0.10 1. 1. 0.10 0.10 0.10 0.10 0.10 Thiamine x HCl 1. 1. 10. 10. 1. 1. 1. 1. 1. Weight (g / L) 8.99 9.28 89.87 92.79 14.44 14.74 8.95 9.24 9.17 749
ME, asal medium with Hanks' salts Inorganic salts Other Components Components A1917 A1313 A1990 A1988 A1918 A1919 A1978 autoclavable Calcium chloride 185.44 185.44 1854.44 1854.44 185.44 185.44 185.44 x2h 2O Magnesium 139.52 139.52 1395.20 1395.20 139.52 139.52 139.52 sulfate dried Potassium 4. 4. 40. 40. 4. 4. 4. chloride Potassium 60. 60. 6. 6. 60. 60. 60. dihydrogen phosphate anhydrous Sodium chloride 80. 80. 8. 8. 75. 75. 80. di-sodium hydrogen phosphate 47.88 47.88 478.80 478.80 47.88 47.88 47.88 D(+)-Glucose 10. 10. 1. 1. 10. 10. 10. anhydrous HEPES 5958. 5958. Phenol red 10. 10. 1. 1. 10. 10. 6. Succinic acid 75. Succinic acid sodium salt 1. Amino acids L-Arginine x HCl 21. 21. 210. 210. 21. 21. 21. L-Cystine 12. 12. 120. 120. 12. 12. 12. L-Glutamine 0 292. 0 2920. 0 292. 0 L-Histidine ase 8. 8. 80. 80. 8. 8. L-Histidine x HCl 15. xh2o L-Isoleucine 26. 26. 260. 260. 26. 26. 26. L-Leucine 26. 26. 260. 260. 26. 26. 26. L-Lysine x HCl 36.47 36.47 364.70 364.70 36.47 36.47 36.47 L-Methionine 7.50 7.50 75. 75. 7.50 7.50 7.50 L-Phenylalanine 16.50 16.50 165. 165. 16.50 16.50 16.50 L-Threonine 24. 24. 240. 240. 24. 24. 24. L-Tryptophan 4. 4. 40. 40. 4. 4. 4. L-Tyrosine 18. 18. 180. 180. 18. 18. 18. L-Valine 23.50 23.50 235. 235. 23.50 23.50 23.50 Vitamins D(+)-iotin 1. 1. 10. 10. 1. 1. 1. D-Calcium 1. 1. 10. 10. 1. 1. 1. pantothenate Choline bitartrate 1.80 Choline chloride 1. 1. 10. 10. 1. 1. Folic acid 1. 1. 10. 10. 1. 1. 1. myo-inositol 2. 2. 20. 20. 2. 2. 2. Nicotinamide 1. 1. 10. 10. 1. 1. 1. Pyridoxal x HCl 1. 1. 10. 10. 1. 1. 1. Riboflavin 0.10 0.10 1. 1. 0.10 0.10 0.10 Thiamine x HCl 1. 1. 10. 10. 1. 1. 1. Weight (g / L) 10.07 10.37 1.75 103.67 15.53 15.82 10.25 750
DMEM - Media Inorganic salts Other Components Components A1924 A1314 A1925 A1315 A0826 A1926 A1316 A1985 A1986 A1950 A1965 A1979 A1951 A3134 A3137 A3139 Calcium chloride x 264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92 264.92 2H 2O Iron(III)-nitrate x 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 9H 2O Magnesium sulfate 139.57 139.57 139.57 139.57 139.57 139.57 139.57 139.57 139.57 139.57 139.57 139.57 139.57 139.57 139.57 139.57 dried Potassium chloride 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. Sodium chloride 64.- Sodium dihydrogen phosphate x H 2O D(+)-Glucose anhydrous 64.- 59.- 59.- 64.- 64.- 64.- 64.- 64.- 54.- 54.- 54.- 54.- 64.- 64.- 64.- 125. 125. 125. 125. 125. 125. 125. 125. 125. 125. 125. 125. 125. 125. 125. 125. 10.- 10.- 10.- HEPES 5958.- 10.- 5958.- 10.- 45.- 45.- 45.- 45.- 45.- 0 5958.- Phenol red 15. 15. 15. 15. 15. 15. 15. 15. 15. 15. 15. 15. Sodium pyruvate 110. 110. 110. 110. 110. 110. 110. 110. 110. 110. 110. 110. Amino acids L-Arginine x HCl 84. 84. 84. 84. 84. 84. 84. 84. 84. 84. 84. 84. 84. 84. 84. 84. L-Cystine 48. 48. 48. 48. 48. 48. 48. 48. 48. 48. 48. 48. 48. 48. 48. 48. L-Glutamine 0 584. 0 584. 0 0 584. 0 584. 0 584. 0 584. 584. 0 584. Glycine 30. 30. 30. 30. 30. 30. 30. 30. 30. 30. 30. 30. 30. 30. 30. 30. L-Histidine x HCl x 42. 42. 42. 42. 42. 42. 42. 42. 42. 42. 42. 42. 42. 42. 42. 42. H 20 L-Isoleucine 105. 105. 105. 105. 105. 105. 105. 105. 105. 105. 105. 105. 105. 105. 105. 105. L-Leucine 105. 105. 105. 105. 105. 105. 105. 105. 105. 105. 105. 105. 105. 105. 105. 105. L-Lysine x HCl 146. 146. 146. 146. 146. 146. 146. 146. 146. 146. 146. 146. 146. 146. 146. 146. L-Methionine 30. 30. 30. 30. 30. 30. 30. 30. 30. 30. 30. 30. 30. 30. 30. 30. L-Phenylalanine 66. 66. 66. 66. 66. 66. 66. 66. 66. 66. 66. 66. 66. 66. 66. 66. L-Serine 42. 42. 42. 42. 42. 42. 42. 42. 42. 42. 42. 42. 42. 42. 42. 42. L-Threonine 95. 95. 95. 95. 95. 95. 95. 95. 95. 95. 95. 95. 95. 95. 95. 95. L-Tryptophan 16. 16. 16. 16. 16. 16. 16. 16. 16. 16. 16. 16. 16. 16. 16. 16. L-Tyrosine 72. 72. 72. 72. 72. 72. 72. 72. 72. 72. 72. 72. 72. 72. 72. 72. L-Valine 94. 94. 94. 94. 94. 94. 94. 94. 94. 94. 94. 94. 94. 94. 94. 94. Vitamins D-Calcium 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. pantothenate Choline chloride 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. Folic acid 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. myo-inositol 7.20 7.20 7.20 7.20 7.20 7.20 7.20 7.20 7.20 7.20 7.20 7.20 7.20 7.20 7.20 7.20 Nicotinamide 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. Pyridoxal x HCl 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. Riboflavin 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 Thiamine x HCl 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. 4. Weight (g / L) 9.46 10.05 14.20 15.50 9.45 12.85 13.44 12.96 13.55 17.81 18.39 17.92 18.50 10.03 12.95 13.53 45.- 5958.- 45.- 5958.- 45.- 5958.- 10.- 45.- 45.- 751
DMEM / F12 - Media Components A1920 A1318 A1922 A1317 A1923 A1921 Inorganic salts Other Components Calcium chloride x 154.45 154.45 154.45 154.45 154.45 154.45 2H 2O Copper(II)-sulfate 0.13 0.13 0.13 0.13 0.13 0.13 x5h 2O Iron(II)-sulfate x 0.417 0.417 0.417 0.417 0.417 0.417 7H2O Iron(III)-nitrate x 0.05 0.05 0.05 0.05 0.05 0.05 9H 2O Magnesium 61. 61. 61. 61. 61. 61. chloride x 6H 2O Magnesium 69.76 69.76 69.76 69.76 69.76 69.76 sulfate dried Potassium 311.80 311.80 311.80 311.80 311.80 311.80 chloride Sodium chloride 6999.50 6999.50 6999.50 6999.50 6999.50 6999.50 Sodium dihydrogen phosphate x H 2O 62.50 62.50 62.50 62.50 62.50 62.50 di-sodium hydrogen phosphate anhydrous Zinc sulfate x 7H 2O 71.02 71.02 71.02 71.02 71.02 71.02 0.432 0.432 0.432 0.432 0.432 0.432 D(+)-Glucose 3151. 3151. 3151. 3151. 3151. 3151. anhydrous HEPES 3574.50 3574.50 5958. 5958. Hypoxanthine 2.10 2.10 2.10 2.10 2.10 2.10 Linoleic acid 0.042 0.042 0.042 0.042 0.042 0.042 DL-alpha-Lipoic 0.105 0.105 0.105 0.105 0.105 0.105 acid Phenol red 8.10 8.10 8.10 8.10 8.10 8.10 Putrescine x 2HCl 0.081 0.081 0.081 0.081 0.081 0.081 Sodium pyruvate 55. 55. 55. 55. 55. 55. Amino acids L-Alanine 4.45 4.45 4.45 4.45 4.45 4.45 L-Arginine x HCl 147.50 147.50 147.50 147.50 147.50 147.50 L-Asparagine x 7.50 7.50 7.50 7.50 7.50 7.50 H2O L-Aspartic acid 6.65 6.65 6.65 6.65 6.65 6.65 L-Cysteine x HCl x 17.56 17.56 17.56 17.56 17.56 17.56 H 20 L-Cystine 24. 24. 24. 24. 24. 24. L-Glutamic acid 7.35 7.35 7.35 7.35 7.35 7.35 anhydrous L-Glutamine 0 365. 0 365. 0 365. Glycine 18.75 18.75 18.75 18.75 18.75 18.75 L-Histidine x HCl x 31.48 31.48 31.48 31.48 31.48 31.48 H 20 L-Isoleucine 54.47 54.47 54.47 54.47 54.47 54.47 L-Leucine 59.05 59.05 59.05 59.05 59.05 59.05 L-Lysine x HCl 91.25 91.25 91.25 91.25 91.25 91.25 L-Methionine 17.24 17.24 17.24 17.24 17.24 17.24 L-Phenylalanine 35.48 35.48 35.48 35.48 35.48 35.48 L-Proline 17.25 17.25 17.25 17.25 17.25 17.25 L-Serine 26.25 26.25 26.25 26.25 26.25 26.25 L-Threonine 53.45 53.45 53.45 53.45 53.45 53.45 L-Tryptophan 9.02 9.02 9.02 9.02 9.02 9.02 L-Tyrosine 38.70 38.70 38.70 38.70 38.70 38.70 L-Valine 52.85 52.85 52.85 52.85 52.85 52.85 Vitamins D(+)-iotin 0.35 0.35 0.35 0.35 0.35 0.35 D-Calcium 2.24 2.24 2.24 2.24 2.24 2.24 pantothenate Choline chloride 8.98 8.98 8.98 8.98 8.98 8.98 2'-Deoxythymidine 0.365 0.365 0.365 0.365 0.365 0.365 Folic acid 2.65 2.65 2.65 2.65 2.65 2.65 myo-inositol 12.60 12.60 12.60 12.60 12.60 12.60 Nicotinamide 2.02 2.02 2.02 2.02 2.02 2.02 Pyridoxal x HCl 2. 2. 2. 2. 2. 2. Pyridoxine HCl 0.031 0.031 0.031 0.031 0.031 0.031 Riboflavin 0.219 0.219 0.219 0.219 0.219 0.219 Thiamine x HCl 2.17 2.17 2.17 2.17 2.17 2.17 Vitamin 12 0.68 0.68 0.68 0.68 0.68 0.68 Weight (g / L) 11.70 12.07 15.28 15.64 17.66 18.02 752
Earle's balanced salts Components A1712 A2039 A3026 A3127 Inorganic salts Calcium chloride x 2H 2O 264.92 264.92 Magnesium sulfate dried 139.57 139.57 Potassium chloride 4. 4. 4. 4. Sodium chloride 68. 68. 68. 68. Sodium dihydrogen phosphate x 140. 140. 140. 140. H2O Other D(+)-Glucose anhydrous 10. 10. 10. 10. Components Phenol red 10. 10. Weight (g / L) 8.75 8.74 8.35 8.34 Gamborg's 5 Plant salts Components A1911 Inorganic salts Ammonium sulfate 134. oric acid 3. Calcium chloride x 2H 2O 149.98 Cobalt(II)-chloride x 6H 2O 0.025 Copper(II)-sulfate x 5H 2O 0.025 Iron(II)-sulfate x 7H2O 27.80 Magnesium sulfate dried 172.11 Manganese(II)-sulfate x H2O 10. Potassium iodide 0.75 Potassium nitrate 25. Sodium dihydrogen phosphate x H 2O 150. Sodium molybdate x 2H 2O 0.25 Zinc sulfate x 7H2O 2. Vitamins D-Calcium pantothenate 0.874 myo-inositol 1. Nicotinamide 1. Pyridoxal x HCl 1. Riboflavin 0.015 Thiamine x HCl 10. Weight (g / L) 3.26 753
Glasgow - MEM (HK 21) Components A2024 A1320 A2025 A1321 Inorganic salts Calcium chloride x 2H 2O 264.92 264.92 238.43 238.43 Iron(III)-nitrate x 9H 2O 0.10 0.10 0.09 0.09 Magnesium sulfate dried 139.52 139.52 125.64 125.64 Potassium chloride 4. 4. 360. 360. Sodium chloride 64. 64. 6260. 6260. Sodium dihydrogen phosphate x 124. 124. 111.60 111.60 H2O di-sodium hydrogen phosphate 250. 250. Other D(+)-Glucose anhydrous 45. 45. 4250. 4250. Components Peptone from casein 10. 10. Peptone from meat 5. 5. Phenol red 15. 15. 13.50 13.50 Yeast extract 5. 5. Amino acids L-Arginine x HCl 42. 42. 37.80 37.80 L-Cystine 24. 24. 21.60 21.60 L-Glutamine 0 292. 0 262.80 L-Histidine x HCl x H 2O 21. 21. 18.90 18.90 L-Isoleucine 52.40 52.40 47.16 47.16 L-Leucine 52.40 52.40 47.16 47.16 L-Lysine x HCl 73.10 73.10 65.79 65.79 L-Methionine 15. 15. 13.50 13.50 L-Phenylalanine 33. 33. 29.70 29.70 L-Threonine 47.60 47.60 42.84 42.84 L-Tryptophan 8. 8. 7.20 7.20 L-Tyrosine 36.20 36.20 32.52 32.52 L-Valine 46.80 46.80 42.12 42.12 Vitamins D-Calcium pantothenate 2. 2. 1.80 1.80 Choline chloride 2. 2. 1.80 1.80 Folic acid 2. 2. 1.80 1.80 myo-inositol 3.60 3.60 3.24 3.24 Nicotinamide 2. 2. 1.80 1.80 Pyridoxal x HCl 2. 2. 1.80 1.80 Riboflavin 0.20 0.20 0.18 0.18 Thiamine x HCl 2. 2. 1.80 1.80 Weight (g / L) 12.31 12.60 14.03 14.29 754
Grace's - Insect media Components A2023 A1529 Inorganic salts Calcium chloride x 2H 2O 1324.60 1324.60 Magnesium chloride x 6H 2O 2278.86 2278.86 Magnesium sulfate dried 1939.81 1939.81 Potassium chloride 2240. 2240. di-sodium hydrogen phosphate 876.92 876.92 Other Components D(+)-Glucose anhydrous 7. 7. D(-)-Fructose 4. 4. Fumaric acid 55. 55. a-ketoglutaric acid sodium salt 425.66 425.66 DL-Malic acid 670. 670. Succinic acid 60. 60. D(+)-Sucrose 26680. 26680. Amino acids L-Alanine 2. 2. L-Arginine x HCl 7. 7. L-Asparagine x H 2O 350. 350. L-Aspartic acid 350. 350. L-Cystine 19.18 19.18 L-Glutamic acid anhydrous 6. 6. L-Glutamine 0 6. Glycine 650. 650. L-Histidine ase 25. 25. L-Isoleucine 50. 50. L-Leucine 75. 75. L-Lysine x HCl 625. 625. L-Methionine 50. 50. L-Phenylalanine 150. 150. L-Proline 350. 350. L-Serine 550. 550. L-Threonine 175. 175. L-Tryptophan 1. 1. L-Tyrosine 50. 50. L-Valine 1. 1. Vitamins p-aminobenzoic acid 0.02 0.02 D(+)-iotin 0.01 0.01 D-Calcium pantothenate 0.02 0.02 Choline chloride 0.20 0.20 Folic acid 0.02 0.02 myo-inositol 0.02 0.02 Nicotinic acid 0.02 0.02 Pyridoxine x HCl 0.02 0.02 Riboflavin 0.02 0.02 Thiamine x HCl 0.02 0.02 Weight (g / L) 45.52 46.12 755
Ham's F10 - Media Components A1970 A1525 A1989 A1982 A2026 A1952 Inorganic salts Other Components Calcium chloride x 2H 2O Copper(II)-sulfate x5h 2O Iron(II)-sulfate x 7H2O Magnesium sulfate dried Potassium chloride Potassium dihydrogen phosphate 44.09 44.09 44.09 44.09 44.09 44.09 0.25 0.25 0.25 0.25 0.25 0.25 0.834 0.834 0.834 0.834 0.834 0.834 106.06 106.06 106.06 106.06 106.06 106.06 285. 285. 285. 285. 285. 285. 83. 83. 83. 83. 83. 83. Sodium chloride 74. 74. 69. 69. 69. 69. di-sodium hydrogen phosphate 153.70 153.70 153.70 153.70 153.70 153.70 Zinc sulfate x 7H 2O 0.029 0.029 0.029 0.029 0.029 0.029 D(+)-Glucose 11. 11. 11. 11. 11. 11. anhydrous HEPES 5958. 5958. 5958. 5958. 2'-Deoxythymidine 0.73 0.73 0.73 0.73 0.73 0.73 Hypoxanthine 4.08 4.08 4.08 4.08 4.08 4.08 DL- 0.21 0.21 0.21 0.21 0.21 0.21 Phenol red 1.20 1.20 1.20 1.20 1.20 1.20 Sodium hydrogen 12. 12. carbonate Sodium pyruvate 110. 110. 110. 110. 110. 110. Amino acids L-Alanine 8.91 8.91 8.91 8.91 8.91 8.91 L-Arginine x HCl 211. 211. 211. 211. 211. 211. L-Asparagine x 15. 15. 15. 15. 15. 15. H 2O L-Aspartic acid 13.30 13.30 13.30 13.30 13.30 13.30 L-Cysteine x HCl x 35.12 35.12 35.12 35.12 35.12 35.12 H2O L-Glutamic acid 14.70 14.70 14.70 14.70 14.70 14.70 anhydrous L-Glutamine 0 146.20 0 146.20 0 146.20 Glycine 7.51 7.51 7.51 7.51 7.51 7.51 L-Histidine x HCl x 21. 21. 21. 21. 21. 21. H2O L-Isoleucine 2.60 2.60 2.60 2.60 2.60 2.60 L-Leucine 13.10 13.10 13.10 13.10 13.10 13.10 L-Lysine x HCl 29.30 29.30 29.30 29.30 29.30 29.30 L-Methionine 4.48 4.48 4.48 4.48 4.48 4.48 L-Phenylalanine 4.96 4.96 4.96 4.96 4.96 4.96 L-Proline 11.50 11.50 11.50 11.50 11.50 11.50 L-Serine 10.50 10.50 10.50 10.50 10.50 10.50 L-Threonine 3.57 3.57 3.57 3.57 3.57 3.57 L-Tryptophan 0.60 0.60 0.60 0.60 0.60 0.60 L-Tyrosine 1.81 1.81 1.81 1.81 1.81 1.81 L-Valine 3.50 3.50 3.50 3.50 3.50 3.50 Vitamins D(+)-iotin 0.024 0.024 0.024 0.024 0.024 0.024 D-Calcium 0.715 0.715 0.715 0.715 0.715 0.715 pantothenate Choline chloride 0.698 0.698 0.698 0.698 0.698 0.698 Folic acid 1.32 1.32 1.32 1.32 1.32 1.32 myo-inositol 0.541 0.541 0.541 0.541 0.541 0.541 Nicotinamide 0.615 0.615 0.615 0.615 0.615 0.615 Pyridoxine x HCl 0.21 0.21 0.21 0.21 0.21 0.21 Riboflavin 0.376 0.376 0.376 0.376 0.376 0.376 Thiamine x HCl 1.01 1.01 1.01 1.01 1.01 1.01 Vitamin 12 1.36 1.36 1.36 1.36 1.36 1.36 Weight (g / L) 9.71 9.85 15.17 15.31 16.37 16.51 756
Ham's F12 - Media Components A1948 A1526 A1969 A1953 Inorganic salts Calcium chloride x 2H 2O 44. 44. 44. 44. Copper(II)-sulfate x 5H 2O 0.3 0.3 0.3 0.3 Iron(II)-sulfate x 7H 2O 0.834 0.834 0.834 0.834 Magnesium chloride x 6H 2O 122. 122. 122. 122. Potassium chloride 223.60 223.60 223.60 223.60 Sodium chloride 7599. 7599. 7099. 7099. di-sodium hydrogen phosphate 142.04 142.04 142.04 142.04 Zinc sulfate x 7H 2O 0.863 0.863 0.863 0.863 Other D(+)-Glucose anhydrous 1801.60 1801.60 1801.60 1801.60 Components HEPES 5958. 5958. 2'-Deoxythymidine 0.73 0.73 0.73 0.73 Hypoxanthine 4.08 4.08 4.08 4.08 Linoleic acid 0.084 0.084 0.084 0.084 DL-a-Lipoic acid 0.21 0.21 0.21 0.21 Phenol red 1.20 1.20 1.20 1.20 Putrescine x 2HCl 0.161 0.161 0.161 0.161 Sodium pyruvate 110.10 110.10 110.10 110.10 Amino acids L-Alanine 8.91 8.91 8.91 8.91 L-Arginine x HCl 210.70 210.70 210.70 210.70 L-Asparagine x H2O 15.01 15.01 15.01 15.01 L-Aspartic acid 13.31 13.31 13.31 13.31 L-Cysteine x HCl x H2O 35.12 35.12 35.12 35.12 L-Glutamic acid anhydrous 14.71 14.71 14.71 14.71 L-Glutamine 0 146.20 0 146.20 Glycine 7.51 7.51 7.51 7.51 L-Histidine x HCl x H 2O 20.96 20.96 20.96 20.96 L-Isoleucine 3.94 3.94 3.94 3.94 L-Leucine 13.12 13.12 13.12 13.12 L-Lysine x HCl 36.54 36.54 36.54 36.54 L-Methionine 4.48 4.48 4.48 4.48 L-Phenylalanine 4.96 4.96 4.96 4.96 L-Proline 34.53 34.53 34.53 34.53 L-Serine 10.51 10.51 10.51 10.51 L-Threonine 11.91 11.91 11.91 11.91 L-Tryptophan 2.04 2.04 2.04 2.04 L-Tyrosine 5.44 5.44 5.44 5.44 L-Valine 11.71 11.71 11.71 11.71 Vitamins D(+)-iotin 0.7 0.7 0.7 0.7 D-Calcium pantothenate 0.24 0.24 0.24 0.24 Choline chloride 13.96 13.96 13.96 13.96 Folic acid 1.32 1.32 1.32 1.32 myo-inositol 18.02 18.02 18.02 18.02 Nicotinamide 0.037 0.037 0.037 0.037 Pyridoxine x HCl 0.062 0.062 0.062 0.062 Riboflavin 0.038 0.038 0.038 0.038 Thiamine x HCl 0.34 0.34 0.34 0.34 Vitamin 12 1.36 1.36 1.36 1.36 Weight (g / L) 10.55 10.70 16.01 16.16 757
Ham's F12K - Media Components A1963 A1927 Inorganic salts Calcium chloride x 2H 2O 135.24 135.24 Copper(II)-sulfate x 5H 2O 0.25 0.25 Iron(II)-sulfate x 7H 2O 0.834 0.834 Magnesium chloride x 6H 2O 105.72 105.72 Magnesium sulfate dried 275.20 275.20 Potassium chloride 283.29 283.29 Potassium dihydrogen phosphate 58.52 58.52 Sodium chloride 7597.20 7597.20 di-sodium hydrogen phosphate 115.02 115.02 Zinc sulfate x 7H 2O 0.1437 0.1437 Other Components D(+)-Glucose anhydrous 1260. 1260. 2'-Deoxythymidine 0.7266 0.7266 Hypoxanthine 4.08 4.08 DL-a-Lipoic acid 0.2063 0.2063 Phenol red 3. 3. Putrescine x 2HCl 0.3222 0.3222 Sodium pyruvate 220. 220. Amino acids L-Alanine 17.82 17.82 L-Arginine x HCl 421.40 421.40 L-Asparagine x H2O 30.02 30.02 L-Aspartic acid 26.62 26.62 L-Cysteine x HCl x H2O 70.24 70.24 L-Glutamic acid anhydrous 29.42 29.42 L-Glutamine 0 292.20 Glycine 15.01 15.01 L-Histidine x HCl x H 2O 41.92 41.92 L-Isoleucine 7.87 7.87 L-Leucine 26.24 26.24 L-Lysine x HCl 73.04 73.04 L-Methionine 8.95 8.95 L-Phenylalanine 9.91 9.91 L-Proline 69.06 69.06 L-Serine 21.02 21.02 L-Threonine 23.82 23.82 L-Tryptophan 4.08 4.08 L-Tyrosine 10.87 10.87 L-Valine 23.42 23.42 Vitamins D(+)-iotin 0.0732 0.0732 D-Calcium pantothenate 0.477 0.477 Choline chloride 13.96 13.96 Folic acid 1.3242 1.32 myo-inositol 18.02 18.02 Nicotinamide 0.0366 0.0366 Pyridoxine x HCl 0.0617 0.0617 Riboflavin 0.0376 0.0376 Thiamine x HCl 0.3373 0.3373 Vitamin 12 1.3554 1.3554 Weight (g / L) 11.03 11.32 Hanks' balanced salts Components A1711 A3140 A2729 A2512 Inorganic salts Calcium chloride x 2H 2O 185.44 185.44 Magnesium sulfate dried 139.52 139.52 Potassium chloride 4. 4. 4. 4. Potassium dihydrogen 60. 60. 60. 60. phosphate Sodium chloride 80. 80. 80. 80. di-sodium hydrogen phosphate 47.88 47.88 47.88 47.88 anhydrous Other D(+)-Glucose anhydrous 10. 10. 10. 10. Components Phenol red 10. 10. Weight (g / L) 9.84 9.83 9.52 9.51 758
IPL-41 - Media Components A2027 A1964 Inorganic salts Ammonium heptamolybdate x 4H 2O 0.04 0.04 Calcium chloride x 2H 2O 662.30 662.30 Cobalt(II)-chloride x 6H 2O 0.05 0.05 Cupric chloride x 6H 2O 0.20 0.20 Iron(II)-sulfate x 7H2O 0.55 0.55 Magnesium sulfate dried 1311.40 1311.40 Manganese chloride x 4H2O 0.02 0.02 Potassium chloride 12. 12. Sodium chloride 2850. 2850. Sodium dihydrogen phosphate x H 2O 1160. 1160. Tin chloride 0.04 0.04 Other Components D(+)-Glucose anhydrous 25. 25. Fumaric acid 4.40 4.40 a-ketoglutaric acid sodium salt 34.05 34.05 DL-Malic acid 53.60 53.60 D(+)-Maltose x H 2O 1052.58 1052.58 Succinic acid 4.80 4.80 D(+)-Sucrose 1650. 1650. Amino acids b-alanine 3. 3. L-Arginine x HCl 8. 8. L-Asparagine x H2O 1477.14 1477.14 L-Aspartic acid 13. 13. L-Cystine 1. 1. L-Glutamic acid anhydrous 15. 15. L-Glutamine 0 10. Glycine 2. 2. L-Histidine ase 2. 2. L-Hydroxyproline 8. 8. L-Isoleucine 750. 750. L-Leucine 250. 250. L-Lysine x HCl 7. 7. L-Methionine 10. 10. L-Phenylalanine 10. 10. L-Proline 5. 5. L-Serine 2. 2. L-Threonine 2. 2. L-Tryptophan 1. 1. L-Tyrosine 250.02 250.02 L-Valine 5. 5. Vitamins p-aminobenzoic acid 0.32 0.32 D(+)-iotin 0.16 0.16 D-Calcium pantothenate 0.8 0.8 Choline chloride 20. 20. Folic acid 0.08 0.08 myo-inositol 0.40 0.40 Nicotinamide 0.16 0.16 Nicotinic acid 0.16 0.16 Pyridoxine x HCl 0.40 0.40 Riboflavin 0.08 0.08 Thiamine x HCl 0.08 0.08 Vitamin 12 0.24 0.24 Weight (g / L) 24.63 25.63 759
Iscove's modified Dulbecco's - Media (IMDM) Components A2028 A1909 A2029 A1322 Inorganic salts Calcium chloride x 2H 2O 218.56 218.56 218.56 218.56 Magnesium sulfate dried 139.52 139.52 139.52 139.52 Potassium chloride 330. 330. 330. 330. Potassium nitrate 0.0760 0.0760 0.0760 0.0760 Sodium chloride 55. 55. 4505. 4505. Sodium dihydrogen phosphate x 125. 125. 125. 125. H2O Sodium selenite anhydrous 0.0110 0.0110 0.0110 0.0110 Other D(+)-Glucose anhydrous 45. 45. 45. 45. Components HEPES 5958. 5958. Phenol red 15. 15. 15. 15. Sodium pyruvate 110. 110. 110. 110. Amino acids L-Alanine 25. 25. 25. 25. L-Arginine x HCl 84. 84. 84. 84. L-Asparagine x H 2O 28.40 28.40 28.40 28.40 L-Aspartic acid 30. 30. 30. 30. L-Cystine 70. 70. 70. 70. L-Glutamic acid anhydrous 75. 75. 75. 75. L-Glutamine 0 584. 0 584. Glycine 30. 30. 30. 30. L-Histidine x HCl x H2O 42. 42. 42. 42. L-Isoleucine 105. 105. 105. 105. L-Leucine 105. 105. 105. 105. L-Lysine x HCl 146. 146. 146. 146. L-Methionine 30. 30. 30. 30. L-Phenylalanine 66. 66. 66. 66. L-Proline 40. 40. 40. 40. L-Serine 42. 42. 42. 42. L-Threonine 95. 95. 95. 95. L-Tryptophan 16. 16. 16. 16. L-Tyrosine 74.86 74.86 74.86 74.86 L-Valine 94. 94. 94. 94. Vitamins D(+)-iotin 0.0130 0.0130 0.0130 0.0130 D-Calcium pantothenate 4. 4. 4. 4. Choline chloride 4. 4. 4. 4. Folic acid 4. 4. 4. 4. myo-inositol 7.20 7.20 7.20 7.20 Nicotinamide 4. 4. 4. 4. Pyridoxal x HCl 4. 4. 4. 4. Riboflavin 0.40 0.40 0.40 0.40 Thiamine x HCl 4. 4. 4. 4. Vitamin 12 0.0130 0.0130 0.0130 0.0130 Weight (g / L) 11.67 12.26 17.13 17.72 760