12V 20 W. 2 1~8 Narrow Band Interference Filters Additional Aspirate Read. Contrast 5. Header. Work area. Message bar.

Similar documents
Brooklyn College Department of Chemistry

CEDIA CYCLOSPORINE PLUS (LOW RANGE) APPLICATION BECKMAN COULTER AU480 /AU680 /AU5800

Simple Operation Manual

Direct Determination of Aluminium in Milk by Graphite Furnace Atomic Absorption Spectrometry

Instructor s Advance Preparation

Direct Determination of Cadmium and Lead in honey by Electrothermal Atomization Atomic Absorption Spectrometry with Zeeman Effect Correction

NOTICE: This method: The laboratories have to study the instrumental conditions appropriate for their own instrumentation.

Determining the Concentration of a Solution: Beer s Law

USGS Troy WSC Laboratory Ion Chromatography SOP EPA 300.0, Rev Jordan Road SOP. No. 1 Rev. No. 1.7 Troy, NY Date 01/25/18 Page 1 of 7

Kinetics of Crystal Violet Bleaching

Technical Procedure for Glass Refractive Index Measurement System 3 (GRIM 3)

User Defined Assay Reference Guide

Predictor Assay Setup Guide on the BMG LABTECH CLARIOstar Microplate Readers

The Determination of an Equilibrium Constant

Introduction. Concepts Kinetics Order of reaction Reaction rate Colorimetry. Background

STANDARD OPERATING PROCEDURE (SOP) FOR ESTIMATION OF DIETARY INTAKE OF SODIUM (DIS) IN 24 HOUR URINE USING ELECTROLYTE ANALYZER

ELISA QUALITY ASSURANCE Analytical Phase

Predictor Assay Setup Guide on the Tecan Safire 2 Microplate Reader

Experiment 2: The Beer-Lambert Law for Thiocyanatoiron (III)

Determining the Concentration of a Solution: Beer s Law. Evaluation copy. Figure 1

Gentian Canine CRP Immunoassay Application Note for ABX Pentra * 400

Determining the Concentration of a Solution: Beer s Law

Rate Law Determination of the Crystal Violet Reaction. Evaluation copy

GeNei TM Enzyme Kinetics Teaching Kit Manual

[C] [D] K eq = [A] [B]

RATE LAW DETERMINATION OF CRYSTAL VIOLET HYDROXYLATION

The Determination of an Equilibrium Constant

2 (aq) [FeSCN [Fe 3JSCN] Figure 1

Excel SOLVER for Fitting Bioanalytical Data to Weighted Polynomial Equations

PREPARATION FOR CHEMISTRY LAB: FLUORIDE IN WATER

Clinical Chemistry Quality Control Policies, Processes, & Procedures

Gentian Canine CRP Immunoassay Application Note for Siemens ADVIA * 1800/1650

SFT3000S Measurement of Sn-Cu Coating

User Defined Assay (UDA) Guide

Determination of the Rate of a Reaction, Its Order, and Its Activation Energy

Spectrophotometry. Uranine Determination. 10-point-calibration for creating an own measurement method with photolab 7600 UV-VIS.

USGS Troy WSC Laboratory Inductively Coupled Plasma- NH4Cl Soil Extracts SOP 425 Jordan Road Rev. No. 2.0 Troy, NY Date: 03/16/2012 Page 1 of 7

425 Jordan Road SOP. No. 10 Rev. No. 1.3 Troy, NY Date: 03/14/2012 Page 1 of 7

ION570. Reference Manual. Ion Analyser D21M073

1iI1E. The Determination of 0 an Equilibrium Constant [LU. Computer

ION570. Reference Manual. Ion Analyser D21M073

The Determination of an Equilibrium Constant

Determining the Concentration of a Solution: Beer s Law

Experiment 2: The Beer-Lambert Law for Thiocyanatoiron (III)

Spectrophotometry Materials

SILICA, Low Range SiLR

Experiment 11 Beer s Law

Experiment 11 Beer s Law

Creating Empirical Calibrations

HbA. Reagent ID: 035 ISE. Range. General. Yes. Test Name: HbA1cΨ

Reviewed by. Joseph V. Bonventre. Document History Date Comment Reviewer Signature 12/20/17 Updated SOP Venkata Sabbisetti

MEASUREMENT: PART II

The Determination of an Equilibrium Constant

CEDIA Tacrolimus Application Ortho Clinical Diagnostics VITROS 5600 Integrated System and VITROS 5,1 FS and 4600 Chemistry Systems

Modified Adams Assay for Phenolics in Wine

TitraLab 580 and 585. KF Titration Workstations. Reference Manual D21T083

Automated determination of the uniformity of dosage in quinine sulfate tablets using a fiber optics autosampler

Standard Operating Procedure. Immunology

SIEMENS DIMENSION VISTA 1 The NGAL Test Reagent Kit

STANDARD OPERATING PROCEDURES

EXPERIMENT 23. Determination of the Formula of a Complex Ion INTRODUCTION

CEDIA Cyclosporine PLUS High Range Application Ortho Clinical Diagnostics VITROS 5600 Integrated System, VITROS 5,1 FS and 4600 Chemistry Systems

WISE Regression/Correlation Interactive Lab. Introduction to the WISE Correlation/Regression Applet

CHEMISTRY 206 Experiment 4: A KINETIC STUDY

Chemical Equilibrium: Finding a Constant, Kc

RINCIPLE OF COLORIMETER AND SPECTOPHOTOMETER AND VARIOUS TYPE OF ANALYSER USED IN CLINICAL BIOCHEMISTRY


BCA Protein Quantitation Kit

Determining the Conductivity of Standard Solutions

A new chapter. medicacorp.com

CEDIA Cyclosporine PLUS Low Range Application Ortho Clinical Diagnostics VITROS 5600 Integrated System and VITROS 5,1 FS and 4600 Chemistry Systems

DETERMINATION OF AN EQUILIBRIUM CONSTANT

Double Star Observations

L-malic acid measurement in wines using the SpectraMax Plus 384 Microplate Reader

Connect the Vernier spectrometer to your lap top computer and power the spectrometer if necessary. Start LoggerPro on your computer.

Photosynthesis. LabQuest OBJECTIVES

EnAlgae SOP sheet: Silicate - SFA. 1. Purpose This procedure is to analyse seawater and freshwater for silicate, SiO2.

V. LAB REPORT. PART I. ICP-AES (section IVA)

Reagent ID: 181. # User Defined * Values set for. Range. General ISE. Test Name: DBIL. Pre-Dilution Rate: 1 Min OD. Volume R1 Volume.

The Clinical Chemistry System that offers more so you do less!

Protein assay of SpectroArt 200

Bio 120 Lab 5: Quantitative Analysis

Conductometric Titration & Gravimetric Determination of a Precipitate

Store User Guide - Forecasting. Jimmy John s

AP Chemistry Laboratory #15: Reaction Rate of Crystal Violet and Sodium Hydroxide. Lab days: Thursday and Friday, February 1-2, 2018

aa + bb cc + dd Equation 1

Concentrations that absorb. Measuring percentage transmittance of solutions at different concentrations

Biology 3B LABORATORY Quantitative determination of chlorophyll using spectroscopy

Chemical Equilibrium: Finding a Constant, Kc

Appendix II- Bioanalytical Method Development and Validation

Acid-Base Titration. Evaluation copy

Saleh Nasiri (Ph.D.) IBTO-Research Center

Reagent ID: 032. # User Defined. * Values set for insert. Range ISE. General. Test Name: Pre-Dilution Rate: 1 Min OD. Max OD

The NGAL Test Reagent Kit

[Unless otherwise stated all tolerances are ±5%] SAMPLES

ICP-OES DETERMINATION OF IRON. Introduction:

3D-Printed Fluidic Devices Enable Quantitative Evaluation of Blood Components in Modified Storage Solutions for Use in Transfusion Medicine

Automated Clinical Analyzer

Bradford Reagent, 5x

MORE LIGHTS, COLOR, ABSORPTION!

Transcription:

12V 20 W 1 10 2 1~8 Narrow Band Interference Filters Additional 10-9 3 58 4 32 Shift Leds Shift + Reset 0.5 Delete 1.5 ml Flow Cell Aspirate Read Rinse Led Exit Enter Reset Contrast 5 Header Work area Message bar Function keys Run Test Screen

6 Start Up Help Home Back Main Menu Soft Ware Basics 6 0~9 Enter 0.5 SEC Calib 700 ~340 Pri wavelen Calibration Screen 7 On 8 Soft Ware RX Monza Enter Flow Cell Main Menu

Start up and perform daily checks Perform gain and pump calibration Select test from the test menu, edit assay parameters if required and press save Enter The Calibration Detail S In The Calibration Menu And Press Save Prepare samples and reagents in test tubes Incubate reaction at the required temperature Place aspiration tube into the reaction mixture Press aspiration switch to begin measurement The absorbance value will be calculated according to the test parameters, and will calculate results based on the stored calibration Retrieve results of samples, calibration and QC data ي جد 192 بزنامج مصنفة في ستة صفحات جشء من ىذه البزامج مبزمج من قبل شزكة راند كس الجشء األخز مفت ح لبزمجتو من قبل مستثمز الجياس 9

None 12 1 Point 2 Point PT-S Blank Rate A Absorbance 340 415 510 546 578 600 660 700 Assay Mode.1.2.3.4 1~999 0 20~40 SEC 999 ~ 2 999 Delay Time 999 ~15 Read Time Unit.5.6.7.8

Format.9 1~99 Replicate: 10MM Flow Cell Cuvette 0~999999 Ref- Low 0~999999 Ref- High 0~999999 Min Lin Lim 0~999999 Max Lin Lim 100 ~ 0 3.0000 ~ -0.25 Min Rx ABS 3.0000 ~ -0.25 Max Rx ABS 3.0000 ~ -0.25 Min Rgt ABS 3.0000 ~ -0.25 Max Rgt ABS 9999 ~ 200 µl ASP VOL 999 ~ 0.1 µl Sample VOL 6500 ~ 1 µl R1 R1 VOL 6500 ~ 0 µl R2 R2 VOL 6500 ~ 0 µl R3 R3 VOL 999999 ~ -999999 Slope A 999999 ~ -999999 Intercept B.10.11.12.13.14.15.16.17.18.19.20.21.22.23.24.25.26.27 Type Of 2 RX Monza Programming Calibration Parameters Parameters Main menu K-Factor Linear Repl 192 Curve

Page Flag Param S0~S11 STD Factor ABS 0.0001 9999999 Conc S0 ~S1 ± Fat Dev% Repl Lim 0 ~3.0000 'R' 0 ~1000 0 ~100 Curve Param Print Save Page Down Up Print Save Run Calib Enter the concentrations of the calibrators in the S0 and S1 fields

3 3 ID 999999 ~ 0.0001 Mean.1 999999 ~ 0.0001 2SD.2 999999 ~ 0.0001 C2 C2 Mean.3 999999 ~ 0.0001 C2 C2 2SD.4 999999 ~ 0.0001 Mean.5 999999 ~ 0.0001 2SD.6 QC

,C2 And QC Daily Graph QC-Daily Data QC-Monthly Graph QC-Monthly Data Accuracy Of Analysis Precision Of Analysis QC- Daily Graph- QC ±3SD SD ±2SD QC N Mean Value X Stanard Drviation SD CV% R Print C2 C2\\ Mthly\Daily Calib Data\ Graph QC-Daily Data

SD Mean 10 Stanard Drviation Mean Value N X SD CV% R SR.NO. Analysis Time Control Results Remark Flag Print C2 C2\\ Mthly\Daily Verify Exclude\ Include Save QC-Monthly Graph

QC 31 QC ±3SD SD ±2SD QC N Mean Value X Stanard Drviation SD CV% R Print C2 C2\\ Mthly\Daily Calib Data\ Graph QC-Monthly Data

QC SR.NO. Analysis Date Control Results Remark Flag Print C2 C2\\ Mthly\Daily Verify Exclude\ Include Save : Run Test Screen Actual temperature attained after flow cell/cuvette blanking Sample Information Sample Information

Assay Calibration Sample Analysis Quality Control Check.1.2.3 Calibration Pre Wavelen Sample VOL Factor Replicates SEC ABS Net Sample ABS Parameter Assay Mode ABS-S0 ASP VOL PRI ABS R2 VOL Sample Number Sample ABS SEC Wavelen Programmed R1VOL Real-Time ABS Sample Blank ABS Calculated % Linearty Sample Result With Flag Water Blank 1 Absorbance Assay Mode Rerun Water Blank Ok 2 Water Blank Fail Sample Aspiration 3

Aspirate Replicate M Of N ASP Of Sample No ##### SKIP Rerun Real Time Absorbance RX curve 4 Skip Print Rerun RX Data Reaction Data Real Time Primary Absorbance 5 Time Real Time Secondary Absorbance Real Time Absorbance

Page Down RX Data PAGE UP Print Rerun Rerun RE-Running Samples Aspirate Replicate 2 Of 3 Of Sample No 10 Skip Skipping Sample Analysis Skip Calibration 6

Full Run Test Calib RX Curve End Of Rerun Curve Cal Data Calibration Rerun RX Curve Reject Accept Curve\Data Sample Analysis 7

Flow-Cell Mode Run Test Screen Enter Aspirate Replicate M Of N Of Sample Number ######### Cuvette Mode Press Read Key For Replicate M Of N Of Sample Number ####### 6 Manual Entery Of Sample Number Aspirate Running Sample In Flow Cell Mode Read Running Sample In Cuvette Mode Read Do You Want To Add R2 Reagent? If Yes Press 1, If No Press 0 Read QC Flag RX CURVE\ RX Data Rerun Skip QC Analysis 8

RX Curve\ Skip Flags QC QC, Run QC Rerun RX Data Using The Flow Cell