NMDA receptor dependent functions of hippocampal networks in spatial navigation and memory formation de Oliveira Cabral, H.

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UvA-DARE (Digital Academic Repository) NMDA receptor dependent functions of hippocampal networks in spatial navigation and memory formation de Oliveira Cabral, H. Link to publication Citation for published version (APA): de Oliveira Cabral, H. (214). NMDA receptor dependent functions of hippocampal networks in spatial navigation and memory formation General rights It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulations If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 112 WP Amsterdam, he Netherlands. You will be contacted as soon as possible. UvA-DARE is a service provided by the library of the University of Amsterdam (http://dare.uva.nl) Download date: 18 Jun 218

1 3 1 3 1,2 1 2 3

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session # trial # surgery pre-training recording 1 2 3 4 5 1 1 2 3 4 15 1 8 15 18 P P P training trial P P P P P P P P P P P probe trial firing rate (Hz) 41.4 31.1 2. 1.5 interneuron pyramidal 16.65 2.5-3 3 time (ms).25 9.63 15.15 2.6 mean AC (msec) -3 3 time (ms) 26.21 31.74 2.55 prob < 7% prob > 7% prob > 8% prob > 9% 71.4 53.92 36.79 19.67 ISVD (%) > ± th

µ µ

a RAINING PLACE SEQUENCE b speed (cm/sec) 4 2 4 2.5 1.5 1 4 2.5 1 4 2.5 1 normalized trial length 4 2 4 2.5 1.5 1 CR KO average velocity (cm/sec) 2 16 12 8 4 CR RAINING PLACE SEQUENCE KO

ISV D = 1 V v V.26 A PV, v.26 v PV %

x P f(x) p(x)f(x)log2 F SpatialInf ormation =, F x x x x Sparsity = (P p(x)f(x)) 2 P (p(x)f(x) 2 ) idx idx idx o o

<

a long short b PLACE SEQUENCE reward localization score c 95 85 75 65 55 CR KO 1 3 5 7 9 pre-training session surgery 1 3 5 7 9 11 13 15 recording session d trials fraction trials fraction CR 1 KO 1 1 2 3 4 5 experiment block PLACE SEQUENCE serial random < x

trials fraction ratio correct choices a b 1.8.6.4.2 1.8.6.4.2 ** ** short long CR KO CR KO CR KO raining PLACE SEQUENCE short 1.8.6.4.2 raining long 1.8.6.4.2 * CR KO ratio correct choices ratio correct choices c 1.8.6.4.2 d 1.8.6.4.2 short short SEQUENCE long 1.8.6.4.2 1.8.6.4.2 PLACE 1.8.6.4.2 long 1.8.6.4.2 *** % e 6 4 2 probability correct outcome f 6 4 % 2 % choice LONG # * % 85 75 65 g * localization score long short * short long pretraining recording 55 pretraining recording 2 < 2 <

2 < 2 < < 2 2 < 2 < < 2 < 2 < < < 2 < 2 <

2 < 2 < < 2 2 < < < <

fraction CR KO area PF.16.8 *** ratio in-field FR increase 12 8 4 *** Hz 6 4 2 max FR # 1.6.8 # of PFs *** bits/spike Spatial Information.8.4 ***.4.2 sparsity *** < < <

9y a CR r PLACE 13.2 8.7 b KO SEQ 7.6 PLACE r 5.5 c SEQ 4.2 72 3.3 2.9 11.1 11.3 3.2 3.8 * ** 6 6 4 4 2 1.8 7.2 d 3. 2. 2.9 1.5 C 2.4 5.1 3.4 R KO 4.2 2 C 2.5 firing rate (Hz) 2.3 R KO 2.3 Sidx 2.5 Pidx 1.4 3 * RAINING PLACE SEQUENCE 2 1 CR 1.9 12.3 2.3 11.3 13.1 e KO 2 CR KO 3.4 21.2 11.1 15.8 Pidx 1 4.8-1 -5 5 15 Sidx 6B;m`2 kxe hq [m MiB7v i?2b2 2z2+ib- r2 + H+mH i2/ irq bbkbh `Biv BM/B+2b, H +2 USidx V M/ b2[m2m+2 BM/2t Uaidx VX h?2 }`bi r b + H+mH i2/ b i?2 S2 `@ bqmƕb +Q``2H ibqm #2ir22M }`BM; ` i2 K b BM i` BMBM; i`b Hb M/ `Q#2 i`b Hb- mbbm; i?2 Qp2`H BM; Q`iBQM Q7 i?2 `Qmi2bX aidx Bb i?2 S2 `bqmƕb +Q``2H ibqm #2ir22M i?2 }`BM; ` i2 K b BM i` BMBM; i`b Hb M/ i?2 }`BM; ` i2 K BM b2[m2m+2@bi` i2;v i`b Hb- `Qi i2/ #v dko iq K F2 i` BMBM; M/ `Q#2 /2 `im`2 `Kb +QBM+B/2X "Qi? +Q``2H ibqmb r2`2 MQ`K HBx2/ #v K2 Mb Q7 b?m 2/ +QM/BiBQM Ub22 J2i?Q/bVX "Qi? *h_ M/ L_R@EP KB+2 b?qr2/ 2H2p i2/ Sidx p Hm2b- b2p2` H@7QH/?B;?2` i? M b?m 2/ +QM@

idx idx < idx idx o idx < < < idx idx < < idx idx < < <

< idx idx < idx idx < idx 8 * SEQ. short SEQ. long idx < S Idx 6 4 2 CR

P idx 1 8 6 4 2 3-5 6-8 9-11 12-15 S idx 6 4 2 3-5 6-8 9-11 session block 12-15 CR KO idx idx idx < < idx < < < idx <

fraction of place cells.8.4 CR 1 2 3 4 5 6 7.8.4 KO Spatial Information (spikes/bin) sessions 1-7 sessions 8-15 1 2 3 4 5 6 7 < < < < < 1 idx < fraction.8.6.4.2 CR KO

r Distribution of P idx in SEQ trials SEQ CR 8.7 5.6 PC fraction.2.15.1.5-1 1.25.2.15.1.5 KO -1 1 n.sig sig r P idx Distribution of S idx in PLACE trials PLACE.2 CR.2 P idx KO 16.8 4.1 PC fraction.15.1.5.15.1.5-1 1 S idx -1 1 S idx th < < idx

idx idx idx

idx idx idx <