Last Amended on 28.03.2014 Page 1 of 8 I. FOOD & AGRICULTURAL PRODUCTS 1. All type of Food and Agri. products Aerobic Plate Count IS: 5402 2012 ISO: 4833 Part 1 2013 Yeast & Mould Count IS: 5403 1999 ISO-21527-2:2008 Coliform Count IS: 5401 (Part 2) 2012 3 MPN/g ISO-4831:2006 ISO-4832:2006 IS: 5401 (Part 1) 2002 E. coli IS: 5887 (Part 1) 1976 ISO-7251:2005 3 MPN/g Detection of S. aureus IS: 5887 (Part 2) 1976 Enumeration of S. aureus IS: 5887 (Part 8) : Sec 1 2002/ ISO 6888 Part 1 2003 Mesophilic spore count Foods APHA 4th Edition 2001 (Chap 22)
Last Amended on 28.03.2014 Page 2 of 8 Flat Sour Spore Count Foods APHA 4th Edition 2001 (Chap 24, 25) Detection of Clostridium perfringens IS: 5887 (Part 4) : 1999 1 10 4 cfu/g Anaerobic spore count Foods APHA 4th Edition 2001 (Chap 23,26) Faecal Streptococci count IS: 5887 (Part 2) 1976 Detection of Vibrio cholerae IS: 5887 (Part 5) 1976 Detection of Vibrio parahaemolyticus IS: 5887 (Part 5) 1976 Detection of Heat Resistant Moulds Foods 4th Edition 2001 (Chap 21) Enumeration of Bacillus cereus IS: 5887 (Part 6) -2012 Enterobacteriaceae Health Protection Agency (2005) National Standard Method F-18 ISO 21528 2 2004
Last Amended on 28.03.2014 Page 3 of 8 Detection of Shigellaspp IS: 5887 (Part 7) 1999 Detection of Salmonellaspp IS: 5887 (Part 3) 1999 ISO: 6579 2002 By VIDAS MC/SOP/MIC/15, Issue No.02, Issue Date: 01-07- 2013 Detection of Listeria monocytogenes IS 14988 (Part 1) 2001 2. Animal Feed products Aerobic Plate Count IS: 5402-2012 ISO: 4833 Part 1-2013 Yeast & Mould Count IS: 5403-1999 ISO-21527-2:2008 Coliform Count IS: 5401 (Part 2) - 2012 3 MPN/g ISO-4831:2006 ISO-4832:2006 IS: 5401 (Part 1) - 2002 E. coli IS: 5887 (Part 1) - 1976 ISO-7251:2005 3 MPN/g Detection of S. aureus IS: 5887 (Part 2) - 1976
Last Amended on 28.03.2014 Page 4 of 8 Enumeration of S. aureus IS: 5887 (Part 8) : Sec 1-2002/ ISO 6888 Part 1 2003 Mesophilic spore count Flat Sour Spore Count Detection of Clostridium perfringens Anaerobic spore count Faecal Streptococci count Detection of Vibrio cholerae Detection of Vibrio parahaemolyticus Detection of Heat Resistant Moulds Foods - APHA 4th Edition 2001 (Chap - 22) Foods - APHA 4th Edition 2001 (Chap - 24, 25) IS: 5887 (Part 4) : 1999 Foods - APHA 4th Edition 2001 (Chap - 23,26) IS: 5887 (Part 2) - 1976 IS: 5887 (Part 5) - 1976 IS: 5887 (Part 5) - 1976 Foods - 4th Edition 2001 (Chap - 21) 1 10 4 cfu/g
Last Amended on 28.03.2014 Page 5 of 8 Enumeration of Bacillus cereus IS: 5887 (Part 6) -2012 Enterobacteriaceae Health Protection Agency (2005) National Standard Method F-18 ISO 21528-2 - 2004 Detection of Shigellaspp IS: 5887 (Part 7) - 1999 Detection of Salmonellaspp IS: 5887 (Part 3) - 1999 ISO: 6579-2002 By VIDAS MC/SOP/MIC/15,Issue No.02, Issue Date: 01-07-2013 II. WATER 1. WATER FROM ANY SOURCE (Drinking, Domestic, Irrigation, Packaged, Process, Mineral, Distilled, Effluent Water, Water used in Processed Food Water, any other water source) Detection of Listeria monocytogenes IS 14988 (Part 1) 2001 Aerobic Plate Count IS: 1622-1981 (Reaffirmed 2009) 1-10 7 cfu/ml IS: 5402 2012 Coliform Count IS: 1622-1981 (Reaffirmed 2009) IS: 5401 (Part 1) - 2002 IS: 15185 2002 1-10 7 cfu/ml 1 MPN /100ml 2 MPN /100ml 1-10 7 cfu/ml E. coli IS: 1622-1981 (Reaffirmed 2009) IS: 5887 (Part 1) - 1976
Last Amended on 28.03.2014 Page 6 of 8 Proteolytic organisms IS 4251-1967 (Reaffirmed 2009) 1-10 7 cfu/ml Lipolytic organisms IS 4251-1967 (Reaffirmed 2009) 1-10 7 cfu/ml Thermophilic Bacteria Count Sulphite Reducing Anaerobes IS 4251-1967 (Reaffirmed 2009) Annexure C, IS: 13428-2005 1-10 7 cfu/ml Pseudomonas aeruginosa Annexure D, IS: 13428-2005 IS: 14543-2004 Yeast & Mould Count IS: 5403-1999 1-10 7 cfu/ml Salmonellaspp IS: 15187-2002 Shigellaspp IS: 5887 (Part 7) - 1999 Faecal Streptococci IS: 5887 (Part 2) - 1976 IS: 15186-2002 Staphylococcus aureus IS: 5887 (Part 2) - 1976 Vibrio parahaemolyticus IS: 5887 (Part 5) - 1976 1 MPN /100ml 2 MPN /100ml 1-10 7 cfu/ml
Last Amended on 28.03.2014 Page 7 of 8 Vibrio cholerae IS: 5887 (Part 5) - 1976 MS2 Phage USEPA method 1602 Cryptosporidium ISO 15553 : 2006 Giardia ISO 15553 : 2006 III. ENVIRONMENT & POLLUTION 1. Food and Other Industrial environmental monitoring Air in factory locations, units, storehouses, dairies, kitchens, any food handling locations Air Sampling (i) Bacterial Count (ii) Yeast & Mould Count MC/SOP/MIC/11,Issue No.02,Issue Date: 30-09-2012 (based on ISO 7218) 1-10 3 cfu/min 1-10 3 cfu/plate 2. Food and Packaging Industries - environmental monitoring Sanitization efficiency of equipments, handlers, linen, cooking platforms, cooking accessories Swabs (i) Bacterial Count (ii) Yeast & Mould Count (iii) Coliform Count (iv) E.coli count MC/SOP/MIC/06,Issue No.01, Issue Date:04-06-2012 1-10 7 cfu/swab 3. Antiseptics and Disinfectants: 1. Soap Bars 2. Liquid Soap Antimicrobial efficacy testing MC/SOP/MIC/17,Issue No.02, Issue Date: 03-01-2014 (based on BS EN 1040:2005) Log reduction:3-5 for acceptability
Last Amended on 28.03.2014 Page 8 of 8 IV MOLECULAR ANALYSIS GMO TESTING 1 Soya & Soya based products Qualitative PCR test for 35 S promoter and NOS terminator. MC/SOP/GMO/03, Issue No.02, Issue Date: 05-08-2013 (based on IS/ISO 21569:2005, Reaffirmed - 2012) Qualitative,, LOD 0.1% 2 Cotton and Cotton based products Qualitative PCR test for 35 S promoter and NOS terminator. MC/SOP/GMO/04, Issue No.02, Issue Date:03-10-2013 (based on IS/ISO 21569:2005, Reaffirmed - 2012) Qualitative,, LOD 0.1% 3 Maize and Maize based product Qualitative PCR test for 35 S promoter and NOS terminator. MC/SOP/GMO/05, Issue No.02, Issue Date:10-11-2013 (based on IS/ISO 21569:2005, Reaffirmed - 2012) Qualitative,, LOD 0.1% -X-X-X-X-X-X-X-X-X-X-X-X-X-X-X-X- *NOTE: The Laboratory has demonstrated competence for the stated scope for WATER. This however does not fully cover the specification requirements of BIS for the Packaged Drinking Water as per IS:14543 and the Packaged Natural Mineral Water IS:13428.