HPLC Products. New 1.9μm Pinnacle DB Small Particle HPLC Columns. 2007/08 Edition. Chromatography Products. hplc columns.

HPLC Products 2007/08 Edition hplc columns hplc accessories hplc replacement parts analytical reference materials vials & syringes New.9μm Pinnacle DB Small Particle HPLC Columns See page 8 for details. Chromatography Products

New.9μm Pinnacle DB Small Particle HPLC Columns See page 8 for details. We're At Your Service. customer service 800-356-688 (ext. 3) Ordering Prices and Availability Delivery Information Order Status Return Authorizations (due to ordering errors) 2. technical service 800-356-688 (ext. 4) e-mail: support@restek.com Product Recommendations Applications Information Troubleshooting Solutions Custom Products Return Authorizations (due to technical issues) 3. literature request 800-356-688 (ext. 5) also see our website For customer service or technical service outside the U.S.: please contact your local Restek representative. Ordering Information. CALL: 800-356-688 (ext. 3) or 84-353-300 (ext. 3) Monday Friday 8:00 a.m. 6:00 p.m. Eastern Time 2. FAX: 84-353-309 24-hours a day 3. ON LINE: 24-hours a day To order from outside the U.S.: please contact your local Restek representative. Restek s payment terms are net 30 days. U.S. Terms FOB Bellefonte, PA Insurance and shipping costs are prepaid and added to invoice. Net due in 30 days,.5% late charge-per-month added after 30 days. For your convenience, we accept payment by MasterCard, Visa, or American Express. Prices and specifications subject to change without notice. Orders are subject to acceptance. Shipping Standard orders: UPS & Federal Express Rush orders: UPS Overnight or Federal Express Priority (Others available upon request.) If you do not receive your Air or Ground Track package on time, call our customer service department. We can track your package.

What's New HPLC Columns Small particle HPLC columns: our popular Pinnacle DB packings in.9µm particle size. Pages 8-9. Capillary HPLC columns for micro LC. Page. phidelity C8 columns: ph stable columns up to ph 2. Page 0. Allure Biphenyl columns: enhanced selectivity for unsaturated compounds. Now in both 3µm & 5µm particle sizes. Page 20. Allure AK columns: resolve 3 carbonyl compounds in 5 minutes. Page 2. New phases on Viva Wide Pore silica: Viva Biphenyl and Viva PFP Propyl HPLC columns, Restek manufactured wide pore Viva silica. Page 30. Restek is now your primary source for all Kromasil columns and packings. Pages 32-33. HPLC Accessories Outlet cap, outlet cap & gold seal assembly, and connecting tubing for Agilent instruments. Page 4. Capillary assemblies for Agilent systems. Page 40. Syringes for CTC/Leap instruments. Page 65. Two-way, three-way, prime/purge, and check valves. Page 45. Restek Pack in a Box Kit: everything you need to pack your own analytical HPLC columns. Page 59. Table Of Contents HPLC Columns.................................................2-35 Bulk Packing Materials....................................36-37 HPLC Guard Columns.......................................38-39 HPLC Replacement Parts and Accessories........... 40-59 Technical Tips...................................................60 Vials & Syringes............................................ 6-65 Analytical Reference Materials............................ 65 Applications................................................. 66-96 Compound Index (Applications)........................... 97

2 Column Selection Selecting an HPLC Column Choosing the best column for your application requires consideration of stationary phase chemistry, retention capacity, particle size, and column dimensions. Identifying the best stationary phase for your separation is the most critical step of column selection, and your decision should be based on sample solubility and the chemical differences among the compounds of interest. Figure is a handy tool for stationary phase selection. Becky Wittrig HPLC Products Marketing Manager 6+ years of service! Figure Stationary phase selection. Water or Polar Organics Sample Solubility Nonpolar Organics HPLC COLUMNS ordering note For assistance in selecting an HPLC column, please contact Restek Technical Service at 84-353-300 or 800-356-688 (ext. 4) or support@restek.com. Reversed Phase Acidic Ionic Aqueous C8 IBD Organic Acids Basic Amino Cyano Basix IBD PFP PFP Propyl Neutral Hydrophobic C C4 C8 C8 Phenyl Biphenyl Hydrophilic Aqueous C8 IBD Organic Acids Mixed Basix IBD Normal Phase Silica Cyano Amino IBD Free Literature HPLC Tech Tips Wall Chart Almost everything you need to remember about HPLC, condensed into 3 feet by 2 feet: mobile phase basics, buffers (types, pk a values, ph ranges, formula masses, more), miscibility and solubility chart (invaluable!), system setup and optimization, detector tips, pressure conversion factors, most-used chromatographic equations, column storage essentials. (lit. cat.# 59894A) Reversed phase columns (e.g. alkyl, phenyl, cyano) work well for water-soluble hydrophobic compounds. Some stationary phases incorporate both polar and nonpolar functionality and can be used in either reversed phase or normal phase modes (e.g. Ultra IBD, Allure Basix, and Allure PFP Propyl). While straight chain alkyl (e.g. C8) stationary phases are historically the most commonly used, many newer phases provide better separations. Alkyl phases are best suited for analyzing neutral compounds with a high ratio of carbon:heteroatoms where the major distinction among analytes is their hydrophobicity. However, for analyzing compounds that are highly polar, aromatic, or halogenated, nonalkyl stationary phases often provide significantly better selectivity (Figure 2). Retention capacity is another important consideration and is influenced by surface area and carbon load (% carbon in the packing material). Allure columns were designed for maximum retention using a high density of ligands bonded to a large surface area silica. Ultra, Kromasil, Pinnacle II and Pinnacle DB columns have the same high ligand density, but are more moderately retentive due to their lower surface areas. Surface area is inversely proportional to pore size; thus, larger pore sizes result in less retention. However, wide pore (e.g. 300Å) packings, such as Viva, are ideal when analyzing larger molecules, as a larger pore size is necessary to allow the analytes to fit into the pores. Particle size and column dimensions also influence column choice. In selecting a particle size, the primary consideration is efficiency (plates/meter) versus column pressure. A 3µm column will have approximately 50% more efficiency than a 5µm column, if all other conditions are constant for both columns. As particle size is further decreased (e.g. <2µm), theoretically, efficiencies will increase proportionally, based on the Van Deemter equation (and the usable flow rate range is much wider). Please note that column backpressure also increases as particle size decreases. Column dimensions include internal diameter and length, where the most commonly used internal diameter (ID) for HPLC columns is 4.6mm. In theory, resolution and pressure should be independent of column ID as long as flow rate is adjusted to maintain the same mobile phase linear velocity (flow rate is proportional to column cross-sectional area). Table I shows the approximate optimum flow rates for four column IDs. Table I Approximate optimum flow rates for various analytical column IDs. 5µm Particles 3µm Particles ID (mm) Optimum Flow Rate (ml/min.) Optimum Flow Rate (ml/min.) 4.6.00.5 3.2 0.50 0.73 2. 0.20 0.3.0 0.05 0.07

Column Selection 3 Figure 2 Stationary phase comparison. Silica Surface Si Si Si Si Si Si Silica Surface CH3 Side Si Si Si Si P3 P2 Si CH2 Polar Group P C8 Alkyl phases (e.g. C8) Alkyl-based stationary phases, such as C8, are best suited for analyzing hydrophobic molecules with a high carbon:heteroatom ratio. Alkyl phase with polar functional group An alkyl-based stationary phase with either an embedded polar group (P), a polar side chain (P2), or a polar end-cap (P3), has significantly greater interaction with polar compounds than a traditional alkyl phase. HPLC COLUMNS Si Conventional Propyl Phenyl Si Biphenyl Phenyl & Biphenyl phases Phenyl stationary phases interact with compounds containing aromatic groups or unsaturated bonds through π-π interactions. The biphenyl stationary phase has even greater interaction due to the higher concentration of aromatic rings. Si Si Si Si Si Si Si Si Silica Surface Silica Surface Si Si F F F F F Pentafluorophenyl Propyl N C Cyano Cyano & Fluorinated phases Fluorinated phases (such as the pentafluorophenyl propyl (PFP propyl)) and cyano-based phases interact strongly with basic, nitrogen-containing and halogenated analytes. Si Si Si Si Si Si

4 Physical Characteristics of Restek HPLC Columns Restek End Pore Carbon HPLC Column Cap? Size (Å) load (%) Applications Pinnacle DB C8 Y 40 Hydrophobic C8 phase suitable for analyses of a wide range of compounds, from acidic through slightly basic. Pinnacle DB C8 Y 40 6 Pinnacle DB Cyano Y 40 4 Applications similar to Pinnacle DB C8, but with less hydrophobic retention. Less retention can be useful for shortening analysis time, if resolution is adequate. Suitable for a wide range of compounds, from acidic through slightly basic. Also useful for confirmation of analyses on a C8 or C8 column. Can be used in normal phase or reversed phase mode of separation. Pinnacle DB Phenyl Y 40 5.3 Suitable for polar aromatic compounds, fatty acids, purines and pyrimidines. Pinnacle DB Silica NA 40 NA Normal phase mode of separation. Pinnacle II C8 Y 0 3 Superior general purpose C8 for non-basic analytes. HPLC COLUMNS Pinnacle II PAH Y 0 Maximum resolution of polynuclear aromatic hydrocarbons. Pinnacle II C8 Y 0 7 Superior general purpose C8 for non-basic analytes. Pinnacle II Cyano Y 0 4 Superior general purpose cyano for weakly-basic analytes. Used in either normal or reversed phase analyses. Pinnacle II Phenyl Y 0 6 Superior general purpose phenyl for neutral analytes. Pinnacle II Amino N 0 2 Excellent general purpose amino phase. Excellent choice for carbohydrate analysis. Pinnacle II Biphenyl Y 0 Multiple aromatic ring structures; excellent for explosives. Pinnacle II Silica NA 0 NA Ideal for polar analytes. Allure C8 Y 60 27 Allure Aqueous C8 N 60 Ideal for MS and light-scattering detection of neutral to slightly polar solutes. Separates basic compounds, showing good deactivation; excellent for explosives or steroids. Ideal for analyses that require >90% water in the mobile phase. Excellent for highly water soluble or poorly organic soluble compounds. Excellent for water-soluble vitamins and organic acids. More retention than Ultra Aqueous columns. Allure AK Y 60 Ideal for the analysis of aldehydes and ketones as DNPH derivatives. Allure Basix Y 60 2 Ideal for LC/MS of basic solutes. Excellent for basic pharmaceuticals or other amine-containing compounds. Allure PFP Propyl Y 60 7 Ideal for MS, ELSD, or NPD detection of nucleosides, nucleotides, purines, pyrimidines, or halogenated compounds. Allure Organic Acids N 60 NA Excellent resolution of challenging organic acids. Allure Biphenyl Y 60 23 Multiple ring structure; excellent for aromatic and unsaturated compounds. Increased retention over traditional phenyl phases. Allure Silica NA 60 NA Highly retentive phase for normal phase separation. Ultra C8 Y 00 20 Ultra Aqueous C8 N 00 5 Ultra IBD N 00 2 Ideal for anilines, barbiturates, carbonyls, fat-soluble vitamins, fatty acids, glycerides, phthalates, PTH amino acids, steroids, other acids. Ideal for analyses that require >90% water in the mobile phase. Excellent for highly water soluble or poorly organic soluble compounds. Excellent for water-soluble vitamins and organic acids. A polar group assists in deactivating surface silanols and contributes to unique separation selectivities for acids, bases, zwitterions, and other polar compounds. Ultra C8 Y 00 2 Selectivity and peak shape similar to Ultra C8, but less hydrophobic retention. Ultra C4 Y 00 9 Ideal for peptides and small proteins. Ultra C NA 00 5 Alternative selectivity to Ultra C8 or C8 columns, especially for polar analytes. Shortest chain alkyl phase available for reversed phase separations. Ultra Cyano Y 00 8 Excellent for basic pharmaceuticals, steroids (normal or reversed phase conditions), or other basic compounds. Ultra Phenyl Y 00 0 Ideal for fatty acids, polynuclear aromatic hydrocarbons, purines and pyrimidines, and polar aromatics. Ultra Amino N 00 2 Superior general purpose amino phase. Ideal for carbohydrates. ph ranges and temperature limits: see product listings on pages listed here. Column lifetime will be shorter when operating at ph and/or temperature extremes.

Physical Characteristics of Restek HPLC Columns 5 Chromatographic Properties Similar Phases USP Code Page # Highly base-deactivated spherical silica manufactured by Restek. Monomeric C8 bonding. Hypersil BDS C8, Zorbax Eclipse XDB-C8 L 2 Highly base-deactivated spherical silica manufactured by Restek. Monomeric C8 bonding. Similar to Pinnacle DB C8, but the shorter alkyl chain provides less hydrophobic retention. Hypersil BDS C8 L7 2 Highly base-deactivated spherical silica manufactured by Restek. Cyano bonding. Hypersil BDS Cyano, Zorbax Eclipse XDB-CN L0 3 Highly base-deactivated spherical silica manufactured by Restek. Phenyl bonding. Hypersil BDS Phenyl, Zorbax Eclipse XDB-Phenyl L 3 Highly base-deactivated spherical silica manufactured by Restek. L3 3 Intermediate carbon load and surface area, suitable for a wide range of neutral to acidic compounds. Silica manufactured by Restek. Hypersil ODS, Spherisorb ODS L 4 Proprietary stationary phase; resolves 6 PAHs in US EPA Method 60. Silica manufactured by Restek. Unique 4 Provides shorter retention times for hydrophobic compounds than C8. Silica manufactured by Restek. Hypersil C8, Spherisorb C8 L7 5 More rugged than bare silica for normal phase analyses. Silica manufactured by Restek. Hypersil CPS, Spherisorb Cyano L0 5 Offers unique selectivity versus traditional alkyl chain phases, especially for aromatic compounds. Silica manufactured by Restek. Hypersil Phenyl, Spherisorb Phenyl L 6 Silica manufactured by Restek. Hypersil APS 2 Amino, Spherisorb Amino L8 6 Silica manufactured by Restek. Unique biphenyl phase. Unique L 7 Superior value phase for normal phase separation of polar analytes. Lower retention than Ultra C8. Silica manufactured by Restek. Hypersil Silica L3 7 Most retentive phase for hydrophobic and slightly polar analytes. Mobile phase containing higher percentage of organic modifier contributes to higher sensitivity in ESI-based LC/MS. Highly retentive and selective for reversed phase separations of polar analytes. Highly base deactivated. Compatible with highly aqueous (up to 00%) mobile phases. Highly retentive, highly selective phase, developed specifically for the analysis of aldehydes and ketones as DNPH derivatives. Ultracarb C8, BetaMax Neutral, Discovery C8 L 8 L 9 Unique 2 HPLC COLUMNS Highly retentive phase for analytes containing amino functionality. BetaMax Base, Maxsil CN L0 8 A pentafluorophenyl phase with a propyl spacer. Highly retentive for basic analytes. Excellent for beta-blockers, halogenated compounds, nucleosides, nucleotides, pyridines, pyrimidines, tricyclic antidepressants. Discovery HS F5 9 Single 30cm column performs equally to two C8 columns in series. (AOAC Method 986.3) Unique 20 High purity, highly retentive phase for aromatic and unsaturated compounds. Unique L 20 High purity, highly retentive phase for normal phase separation of polar analytes. Very high surface area. Maxsil Si L3 2 A very retentive, high-purity phase that exhibits excellent peak shape for a wide range of compounds. Recommended as a general purpose reversed phase column. Highly retentive and selective for reversed phase separations of polar analytes. Highly base deactivated. Compatible with highly aqueous (up to 00%) mobile phases. One of a group of intrinsically base-deactivated (IBD) phases, with a polar group within, or intrinsic to, the alkyl bonded phase. Provides unique selectivity and high level of base deactivation while reducing or eliminating the need for mobile phase additives. Very retentive, high-purity, base-deactivated reversed phase packing that exhibits excellent peak shape for a wide range of compounds. Exceptionally stable C4 packing, with high bonding coverage and silanol base-deactivation. Exhibits shorter retention than C8 or C8 phases. Exceptionally stable C packing resists hydrolysis, even under acidic mobile phase conditions. Least retentive reversed phase hydrocarbon packing. High-purity cyano phase with reduced silanol activity. Often a better choice than C8 for basic pharmaceuticals. Cyano is the most stable bonded phase for normal phase mode. High-purity, highly retentive, base-deactivated phase with alternate selectivity to hydrocarbon phases, especially for aromatic analytes. Recommended for normal phase analyses of mono- and disaccharides and other similar compounds. Can also serve as a weak anion exchanger, with aqueous buffers. Discovery C8, Symmetry C8, Hypersil Gold C8, Luna C8, Zorbax C8, Kromasil C8, LiChrospher RP-8, Inertsil ODS-2, Develosil C8 AQUA C8, Aquasil C8, Hypersil Gold AQ, YMC ODS-Aq L 22 L 23 SymmetryShield, Discovery ABZ & ABZ+, Prism 22 Luna C8, Symmetry C8, Hypersil Gold C8 L7 23 Supelcosil Butyl (C4), Delta-Pak C4 L26 24 Spherisorb C L3 24 Platinum CN, Develosil Cyano, Luna CN, Hypersil Gold CN Platinum Phenyl, Supelcosil Phenyl, Betasil Phenyl L0 25 L 25 Platinum Amino, Develosil NH2 L8 26 Continued on next page...

6 Physical Characteristics of HPLC Columns; USP Descriptions Restek HPLC Column End Cap? Pore Size (Å) Carbon load (%) Applications Ultra PFP Y 00 7 Ideal for taxol and precursors, or halogenated compounds, amines, esters, or ketones. Ultra Silica NA 00 NA Ideal for normal phase applications. Ultra Carbamate 00 Rapid analysis of carbamates. Ultra Quat 00 Proprietary phase for the analysis of paraquat and diquat and other quaternary amines. Kromasil C8 Y 00 20 HPLC COLUMNS Kromasil C8 Y 00 2 Kromasil C4 Y 00 8 Kromasil C Y 00 Kromasil Phenyl Y 00 4 Kromasil Amino Y 00.7% nitrogen Kromasil Silica 00 Viva Wide Pore C8 Y 300 9 Proteins and other higher molecular weight compounds. Viva Wide Pore C8 Y 300 5 Proteins and other higher molecular weight compounds. Less retentive than C8 phase. Viva Wide Pore C4 Y 300 3.5 Proteins and other higher molecular weight compounds. Less retentive than C8 and C8 phases. Viva Wide Pore Biphenyl Viva Wide Pore PFP Propyl Y 300 6.7 Exhibits excellent peak shape for a wide range of compounds; ideal for large molecule and biomolecule assays. Y 300 5 Exhibits excellent peak shape for a wide range of compounds, including nucleosides, nucleotides, and halogenated compounds. Viva Wide Pore Silica 300 Normal phase applications for highly retained high molecular weight compounds. phidelity C8 NA 200 Hydrophobic C8 phase suitable for analyzing a wide range of compounds; enhanced stability under basic and acidic conditions. ph ranges and temperature limits: see product listings on pages listed here. Column lifetime will be shorter when operating at ph and/or temperature extremes. US Pharmacopoeia Cross Reference L L3 L7 L8 L0 L L3 L26 L43 Octadecyl silane chemically bonded to porous silica or ceramic microparticles,.7 to 0µm in diameter, or a monolithic rod. Allure C8 (p. 8), Pinnacle DB C8 (p. 2), Pinnacle II C8 (p. 4), Ultra C8 (p. 22), Allure Aqueous C8 (p. 9), Ultra Aqueous C8 (p. 23), Viva C8 (p. 29), Kromasil C8 (p. 32) Porous silica particles, 5 to 0µm in diameter. Allure Silica (p. 2), Pinnacle DB Silica (p. 3), Pinnacle II Silica (p. 7), Ultra Silica (p. 27), Viva Silica (p. 3), Kromasil Silica (p. 33) Octylsilane chemically bonded to totally porous silica particles,.7 to 0µm in diameter. Pinnacle DB C8 (p. 2), Pinnacle II C8 (p. 5), Ultra C8 (p. 22), Viva C8 (p. 29), Kromasil C8 (p. 33) An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 3 to 0µm in diameter. Pinnacle II Amino (p. 6), Ultra Amino (p. 26), Kromasil Amino (p. 33) Nitrile groups chemically bonded to porous silica particles, 3 to 0µm in diameter. Pinnacle DB Cyano (p. 3), Pinnacle II Cyano (p. 5), Ultra Cyano (p. 25), Allure Basix (p. 8) Phenyl groups chemically bonded to porous silica particles,.7 to 0µm in diameter. Pinnacle DB Phenyl (p. 3), Pinnacle II Phenyl (p. 6), Ultra Phenyl (p. 25), Allure Biphenyl (p. 20), Pinnacle II Biphenyl (p. 7), Viva Biphenyl (p. 30), Kromasil Phenyl (p. 33) Trimethylsilane chemically bonded to porous silica particles, 3 to 0µm in diameter. Ultra C (p. 24), Kromasil C (p. 33) Butyl silane chemically bonded to totally porous silica particles, 3 to 0µm in diameter. Ultra C4 (p.24), Viva C4 (p.30), Kromasil C4 (p.32) Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 5 to 0µm in diameter. Allure PFP Propyl (p. 9), Ultra PFP (p. 26), Viva PFP Propyl (p. 30)

Physical Characteristics of HPLC Columns; USP Descriptions 7 Chromatographic Properties A pentafluorophenyl phase. Unique selectivity by interaction with functional groups of organohalogens or other basic analytes. Similar Phases Fluophase PFP, Fluosep -RP Phenyl, Curosil PFP USP Code Page # L43 26 High purity, high surface area. L3 27 Proprietary stationary phase can process up to twice as many samples per hour, compared to a conventional C8 phase. Unique 27 High purity silica. Unique 28 High purity phase with excellent peak shape for a wide range of compounds. Good general pupose reversed phase column. Discovery C8, Symmetry C8, Hypersil Gold C8, Luna C8, Zorbax C8, LiChrospher RP-8, Inertsil ODS-2, Develosil C8 L 32 High purity, reversed phase packing for a wide range of compounds. Luna C8, Symmetry C8, Hypersil Gold C8 L7 33 High purity, reversed phase packing for a wide range of compounds. Less retentive than C8 and C8. Supelcosil Butyl (C4), Delta-Pak C4 L26 32 High purity, reversed phase packing for a wide range of compounds. Less retentive than C8, C8, and C4. Spherisorb C L3 33 High purity, base deactivated reversed phase packing. Alternate selectivity to alkyl phases. Platinum Phenyl, Supelcosil Phenyl, Betasil Phenyl L 33 High purity, base deactivated reversed phase packing. Alternate selectivity to alkyl phases. Platinum Amino, Develosil NH2 L8 33 High purity, base deactivated packing. L3 33 Silica manufactured by Restek. Silica manufactured by Restek. Silica manufactured by Restek. BioBasic 8, Symmetry 300 C8, Jupiter 300 C8, Zorbax 300 OSB C8, Synchropak C8, 208 TP C8 BioBasic 8, Zorbax 300 OSB C8, Synchropak C8, 208 TP C8 BioBasic 4, Symmetry 300 C4, Jupiter 300 C4, Synchropak C4, 208 TP C4 L 29 L7 29 L26 30 HPLC COLUMNS Silica manufactured by Restek. Unique L 30 Silica manufactured by Restek. Unique L43 30 Silica manufactured by Restek. L3 3 Excellent stability under extreme ph conditions. True C8 selectivity in a silica-based stationary phase. Unique 0 tech tip Managing High Backpressure High backpressure is one of the most common problems encountered in HPLC analyses. Normal column backpressure is observed after a new column has been installed and equilibrated with mobile phase. Unfortunately, this pressure often will increase as the column is used because particles collect on the column inlet frit. These particles can be sample impurities, mobile phase contaminants, or materials from the injector or autosampler rotor seal. In addition to increasing backpressure, particles on the frit can cause split peaks, peak tailing, and, eventually, over-pressure shut-down. In some circumstances, these problems can be corrected by back-flushing the column. However, in many cases the result is an unusable column. To minimize backpressure problems, all samples and mobile phase solvents must be filtered before use, and rotor seals should be changed on a routine basis. Along with these preventive measures, it is advisable to use precolumn filters such as the Trident guard column protection system. Particles build up on the inexpensive, replaceable frit in the filter, instead of on the permanent frit at the column inlet. Free Literature HPLC Column Selection Guide A useful chart to keep with your workbooks, or post on a wall. Quickly scan important characteristics of Restek HPLC columns. Includes brief, practical guidelines for choosing stationary phase, particle size, pore diameter, and column dimensions. Also includes USP designations for each phase and lists similar phases from other suppliers. (lit. cat.# 59454C)

8 New.9μm Pinnacle DB C8 Small Particle HPLC Columns free literature HPLC Small Particle Columns Call Restek at 800-356-688 or 84-353-300, ext. 5, or contact your Restek representative, to request your free copy! lit. cat.# 58047A Restek s popular Pinnacle DB HPLC columns are now available in a <2μm particle size. Ruggedness and reproducibility are guaranteed, as we control every step in the process, from base silica to bonded phase to final packed column. The silica particles are classified and selected to give an exceptionally tight distribution around.9µm, while eliminating <µm particles that can contribute to a poorly packed bed. Highly base-deactivated Pinnacle DB stationary phases are an excellent choice when analyzing a wide range of compounds, from acidic to basic. To optimize your ultra high pressure HPLC methods, reach for Restek small particle HPLC columns!.9μm Pinnacle DB C8 HPLC Columns Physical Characteristics: particle size:.9µm endcap: yes pore size: 40Å ph range: 2.5-0 carbon load: % temperature limit: 80 C Chromatographic Properties: Highly base-deactivated spherical silica manufactured by Restek. Monomeric C8 bonding. 2.mm ID Length cat.# price.9µm Columns 30mm 944232 50mm 944252 00mm 94422 Additional phases available June 2007! Visit or call Restek Technical Service for a full listing of our available phases.

Small Particle HPLC Columns 9 Figure Pinnacle DB silica particle size distribution chart shows an exceptionally tight, symmetrical distribution around.9μm with no <μm particles. Rick Lake Innovations Chemist 2+ years of service! HPLC COLUMNS Figure 2 Excellent peak symmetry and efficiency on a.9μm Pinnacle DB C8 column, using a reversed phase test mix.. uracil 3.00 mg/ml 2. benzene 0.02 mg/ml 3. naphthalene 0.50 mg/ml 4. biphenyl 0.06 mg/ml Performance: (calculations for biphenyl) Efficiency: 27,69 n/m Asymmetry:.0 Pressure: 4,500 psi Inj.: 2µL Sample diluent: water:methanol (25:75) Sample temp.: ambient Column: Pinnacle DB C8 Cat. #: 944252 Dimensions: 50 x 2.mm Particle size:.9µm Pore size: 40Å water:acetonitrile (45:55) 0.4 ml/min. Temp.: 25 C (or ambient) UV @ 254nm LC_EX0427

0 phidelity Columns phidelity C8 Columns Stable under extreme ph conditions. Patented technology protects the silica particle from dissolution. True C8 selectivity. HPLC COLUMNS Application Page # Basic Drugs..............94 Serotonin Reuptake Inhibitors...............90 Restek is pleased to offer phidelity C8 columns, designed for analyses that require extreme ph conditions. Using technology patented by Selerity Technologies (US Patent 2005/09503A), phidelity silica-based columns have exceptional stability under aggressive ph conditions. In phidelity columns, a polycarbosilane barrier layer protects the silica particle from extremely basic conditions. This layer, with multiple points of attachment to the silica particle, yields a modified surface with enhanced stability. A second layer is then attached, providing the functional group (C8). Using this approach to shield the silica surface, a highly durable stationary phase is created. Even after 50 hours of exposure to base (ph 0) at 60 C, a phidelity column maintains its original column efficiency. (A decrease in column efficiency indicates the dissolution of support silica and loss from the column.) This exceptional performance ensures phidelity columns offer greater resolution under harsh mobile phase conditions and will continue to resolve your target compounds over a long column lifetime. A phidelity column also maintains a very consistent capacity factor, k, after exposure to ph 0 and 60 C. Under extremely basic conditions, a decrease in retention time indicates loss of both support silica and bonded phase. Backpressure in the phidelity C8 column is very stable, indicating little or no degradation of the packing material. The patented process used to protect the support silica in phidelity columns ensures more consistent retention times over the lifetime of the column. phidelity C8 Columns Physical Characteristics: particle size: 3µm pore size: 40Å ph limit: up to 2 temperature limit: 80 C Chromatographic Properties: Excellent stability under extreme ph conditions. True C8 selectivity in a silica-based stationary phase. 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price 3µm Columns 30mm 9579332 9579333 9579335 50mm 9579352 9579353 9579355 00mm 957932 957933 957935 50mm 9579362 9579363 9579365 ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: 97435-700 Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39.

Capillary LC Columns Restek Capillary HPLC Columns High quality, Restek manufactured packing materials. Superior packing technology ensures rugged, reproducible columns. Wide range of phases and dimensions available please inquire. new! Bruce Albright HPLC Innovations Chemist 4+ years of service! Physical Characteristics: Allure Basix: particle size: 3µm, spherical pore size: 60Å carbon load: 2% endcap: fully endcapped ph range: 2.5 to 7.5 temperature limit: 80 C Allure Biphenyl: particle size: 3µm, spherical pore size: 60Å carbon load: 23% endcap: yes ph range: 2.5 to 7.5 temperature limit: 80 C Allure PFP Propyl: particle size: 3µm, spherical pore size: 60Å carbon load: 7% endcap: fully endcapped ph range: 2.5 to 7.5 temperature limit: 80 C Ultra Aqueous C8: particle size: 3µm, spherical pore size: 00Å endcap: no ph range: 2.5 to 7.5 temperature limit: 80 C Ultra C8: particle size: 3µm, spherical pore size: 00Å carbon load: 20% endcap: fully endcapped ph range: 2.5 to 7.5 temperature limit: 80 C Viva C8: particle size: 3µm, spherical pore size: 300Å carbon load: 9% endcap: yes ph range: 2.5 to 0 temperature limit: 80 C Chromatographic Properties: Available in a wide variety of stationary phases for acidic to basic compounds. Columns are suitable for small molecules using Allure and Ultra phases and large biomolecules using Viva C8. ordering note Looking for another stationary phase or capillary dimension? Please contact Restek Technical Service at 84-353- 300 or 800-356-688 (ext. 4) or support@restek.com to inquire. HPLC COLUMNS 0.3mm ID Length cat.# price Allure Basix 3µm Capillary Columns 50mm 9635B 50mm 9636B Allure Biphenyl 3µm Capillary Columns 50mm 96635B 50mm 96636B Allure PFP Propyl 3µm Capillary Columns 50mm 96935B 50mm 96936B Ultra Aqueous C8 3µm Capillary Columns 50mm 97835B 50mm 97836B Ultra C8 3µm Capillary Columns 50mm 97435B 50mm 97436B Viva C8 3µm Capillary Columns 50mm 95435B 50mm 95436B

2 Pinnacle DB C8 and C8 Columns Pinnacle DB Columns: 3 or 5μm particle sizes; 40Å pore size Prepared using a highly base-deactivated silica support; ideal for analyses of basic compounds, or bases mixed with acids/neutrals. Silica manufactured at Restek, for total control of quality and reproducibility. HPLC COLUMNS Application Page # Vanilla Bean Extract.......8 Xanthines...............95 also available More chromatograms available! Visit or see our annual product catalog. Pinnacle DB C8 Columns (USP L) Physical Characteristics: Restek Manufactured particle size: 3µm or 5µm, spherical endcap: yes Silica pore size: 40Å ph range: 2.5-0 carbon load: % temperature limit: 80 C Chromatographic Properties: Highly base-deactivated spherical silica manufactured by Restek. Monomeric C8 bonding. Hydrophobic C8 phase suitable for analyses of a wide range of compounds, from acidic through slightly basic. Replaces Hypersil BDS C8 and Pinnacle ODS Amine..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 94433 944332 944333 944335 50mm 94435 944352 944353 944355 00mm 9443 94432 94433 94435 5µm Columns 30mm 94453 944532 944533 944535 50mm 94455 944552 944553 944555 00mm 9445 94452 94453 94455 50mm 94456 944562 944563 944565 200mm 94452 944522 944523 944525 250mm 94457 944572 944573 944575 Pinnacle DB C8 Columns (USP L7) Physical Characteristics: Restek Manufactured particle size: 3µm or 5µm, spherical endcap: yes Silica pore size: 40Å ph range: 2.5-0 carbon load: 6% temperature limit: 80 C Chromatographic Properties: Highly base-deactivated spherical silica manufactured by Restek. Monomeric C8 bonding. Similar to Pinnacle DB C8, but the shorter alkyl chain provides less hydrophobic retention. Less retention can be useful for shortening analysis time, if resolution is adequate. Replaces Hypersil BDS C8 and Pinnacle C8 Amine..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 94333 943332 943333 943335 50mm 94335 943352 943353 943355 00mm 9433 94332 94333 94335 5µm Columns 30mm 94353 943532 943533 943535 50mm 94355 943552 943553 943555 00mm 9435 94352 94353 94355 50mm 94356 943562 943563 943565 200mm 94352 943522 943523 943525 250mm 94357 943572 943573 943575 ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: 97435-700 Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39.

Pinnacle DB Cyano, Phenyl, and Silica Columns 3 Pinnacle DB Cyano Columns (USP L0) Physical Characteristics: particle size: 5µm, spherical endcap: yes pore size: 40Å ph range: 2.5-7.5 carbon load: 4% temperature limit: 80 C Restek Manufactured Silica Chromatographic Properties: Highly base-deactivated spherical silica manufactured by Restek. Cyano bonding. Suitable for analyses of a wide range of compounds, from acidic through slightly basic. Also useful for confirmation of analyses on a C8 or C8 column. Can be used in normal phase or reversed phase mode of separation. Replaces Hypersil BDS Cyano and Pinnacle Cyano Amine..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 5µm Columns 30mm 94653 946532 946533 946535 50mm 94655 946552 946553 946555 00mm 9465 94652 94653 94655 50mm 94656 946562 946563 946565 200mm 94652 946522 946523 946525 250mm 94657 946572 946573 946575 Pinnacle DB Phenyl Columns (USP L) Physical Characteristics: particle range: 5µm, spherical endcap: yes pore size: 40Å ph range: 2.5-7.5 carbon load: 5.3% temperature limit: 80 C Restek Manufactured Silica Chromatographic Properties: Highly base-deactivated spherical silica manufactured by Restek. Pinnacle DB Phenyl columns offer alternate selectivity to straight chain hydrocarbon phases, especially for aromatic analytes. Replaces Hypersil BDS Phenyl and Pinnacle Phenyl Amine. HPLC COLUMNS.0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 5µm Columns 30mm 94553 945532 945533 945535 50mm 94555 945552 945553 945555 00mm 9455 94552 94553 94555 50mm 94556 945562 945563 945565 200mm 94552 945522 945523 945525 250mm 94557 945572 945573 945575 Pinnacle DB Silica Columns (USP L3) Physical Characteristics: particle size: 5µm, spherical endcap: no pore size: 40Å ph range: 2.5-0 carbon load: none temperature limit: 80 C Restek Manufactured Silica Chromatographic Properties: Highly base-deactivated spherical silica manufactured by Restek. Useful for normal phase separations. Replaces Hypersil BDS and Pinnacle Amine..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 5µm Columns 30mm 94053 940532 940533 940535 50mm 94055 940552 940553 940555 00mm 9405 94052 94053 94055 50mm 94056 940562 940563 940565 200mm 94052 940522 940523 940525 250mm 94057 940572 940573 940575

4 Pinnacle II C8 and PAH Columns Pinnacle II Columns: 3μm or 5μm particles; 0Å pore size Silica manufactured at Restek, for total control of quality and reproducibility. Excellent replacement for the original Hypersil material. Physical and chromatographic properties similar to our original Pinnacle materials, but with greater lot-to-lot uniformity. HPLC COLUMNS Application Page # Allicin..................77 Capsaicinoids............79 Morphine Sulfate.........85 Phenolic Antioxidants.........77, 79 Pinnacle II C8 Columns (USP L) Physical Characteristics: Restek Manufactured particle size: 3µm or 5µm, spherical endcap: fully endcapped Silica pore size: 0Å ph range: 2.5 to 0 carbon load: 3% temperature limit: 80 C Chromatographic Properties: Excellent choice as a general purpose C8 column. Intermediate carbon loading and surface area, suitable for a wide range of acidic to neutral hydrophobic compounds. Replaces Hypersil ODS and Pinnacle C8..0mm ID 2.mm ID 3.2mm ID 4.0mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 92433 924332 924333 924335 50mm 92435 924352 924353 924355 00mm 9243 92432 92433 92435 5µm Columns 30mm 92453 924532 924533 924535 50mm 92455 924552 924553 924555 00mm 9245 92452 92453 92454 92455 50mm 92456 924562 924563 924564 924565 200mm 92452 924522 924523 924525 250mm 92457 924572 924573 924575 new and Improved! Pinnacle II PAH Columns Physical Characteristics: particle size: 4µm, spherical pore size: 0Å endcap: fully endcapped ph range: 2.5 to 0 temperature limit: 80 C Restek Manufactured Silica Chromatographic Properties: Developed specifically for challenging analyses of polynuclear aromatic hydrocarbons. The Pinnacle II PAH stationary phase incorporates a proprietary C8 bonding that enables unique shape selectivity to resolve to baseline all 6 PAHs listed in US EPA Method 60. Every lot of Pinnacle II PAH bonded phase material is tested to ensure baseline resolution of the Method 60 PAHs, using a simple water/acetonitrile mobile phase gradient. Further, because we make Pinnacle II PAH columns using our own silica, we have greater control over quality and reproducibility. Replaces Pinnacle PAH columns. If you are analyzing PAHs, Pinnacle II PAH columns are the reliable, cost-effective columns you need. Application Page # PAHs...................76 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price 4µm Columns 50mm 929452 929453 929455 00mm 92942 92943 92945 50mm 929462 929463 929465 200mm 929422 929423 929425 250mm 929472 929473 929475 a plus story The Pinnacle II PAH column gives an excellent separation of the 8 target PAHs that we commonly analyze. Column lifetime far exceeded our expectations-after more than 5000 injections, the columns still maintain resolution and peak shape. Other PAH columns typically had lifetimes of 2000 to 2500 injections. We actually had one Pinnacle II PAH column last for more than 0,000 injections! Norm Farmer, Technical Director, Accutest Laboratories

Pinnacle II C8 and Cyano Columns 5 Pinnacle II C8 Columns (USP L7) Physical Characteristics: Restek Manufactured particle size: 3µm or 5µm, spherical endcap: fully endcapped Silica pore size: 0Å ph range: 2.5 to 0 carbon load: 7% temperature limit: 80 C Chromatographic Properties: Reliable performance and symmetrical peaks for neutral to acidic compounds. Provides shorter retention times for hydrophobic compounds, compared to C8 phases. Replaces Hypersil C8 and Pinnacle C8..0mm ID 2.mm ID 3.2mm ID 4.0mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 92333 923332 923333 923335 50mm 92335 923352 923353 923355 00mm 9233 92332 92333 92335 5µm Columns 30mm 92353 923532 923533 923535 50mm 92355 923552 923553 923555 00mm 9235 92352 92353 92354 92355 50mm 92356 923562 923563 923564 923565 200mm 92352 923522 923523 923525 250mm 92357 923572 923573 923575 Pinnacle II Cyano Columns (USP L0) Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 0Å ph range: 2.5 to 7.5 carbon load: 4% temperature limit: 80 C Restek Manufactured Silica Chromatographic Properties: Can be used in either reversed phase or normal phase mode. More rugged than bare silica for normal phase applications. Replaces Hypersil Cyano and Pinnacle CN. Application Page # Parabens...............78 also available More chromatograms available! Visit or see our annual product catalog. HPLC COLUMNS.0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 92633 926332 926333 926335 50mm 92635 926352 926353 926355 00mm 9263 92632 92633 92635 5µm Columns 30mm 92653 926532 926533 926535 50mm 92655 926552 926553 926555 00mm 9265 92652 92653 92655 50mm 92656 926562 926563 926565 200mm 92652 926522 926523 926525 250mm 92657 926572 926573 926575 Application Page # Corticosteroids..........9 Piperine................79 ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: 97435-700 Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39.

6 Pinnacle II Phenyl and Amino Columns HPLC COLUMNS Application Page # Parabens...............78 Sorbic & Benzoic Acids....79 also available More chromatograms available! Visit or see our annual product catalog. Application Page # Lactulose Concentrate............95 Maple Syrup.............8 Sugars.................8 Pinnacle II Phenyl Columns (USP L) Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 0Å ph range: 2.5 to 7.5 carbon load: 6% temperature limit: 80 C Restek Manufactured Silica Chromatographic Properties: The Pinnacle II Phenyl phase offers unique selectivity versus traditional alkyl chain phases, especially for aromatic compounds. Replaces Hypersil Phenyl and Pinnacle Phenyl..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 92533 925332 925333 925335 50mm 92535 925352 925353 925355 00mm 9253 92532 92533 92535 5µm Columns 30mm 92553 925532 925533 925535 50mm 92555 925552 925553 925555 00mm 9255 92552 92553 92555 50mm 92556 925562 925563 925565 200mm 92552 925522 925523 925525 250mm 92557 925572 925573 925575 Pinnacle II Amino Columns (USP L8) Physical Characteristics: particle size: 3µm or 5µm, spherical pore size: 0Å ph range: 2.5 to 7.5 carbon load: 2% temperature limit: 80 C Restek Manufactured Silica Chromatographic Properties: HPLC analysis using an amino-based stationary phase is the most popular technique for routine analyses of simple sugars, using isocratic elution (e.g., acetonitrile:water, 75:25) and a refractive index detector (RID) or an evaporative light scattering detector (ELSD). The Pinnacle II Amino column is ideal for mono- and disaccharide analyses. Replaces Hypersil Amino and Pinnacle Amino..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 92733 927332 927333 927335 50mm 92735 927352 927353 927355 00mm 9273 92732 92733 92735 5µm Columns 30mm 92753 927532 927533 927535 50mm 92755 927552 927553 927555 00mm 9275 92752 92753 92755 50mm 92756 927562 927563 927565 200mm 92752 927522 927523 927525 250mm 92757 927572 927573 927575 ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: 97435-700 Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39.

Pinnacle II Biphenyl and Silica Columns 7 Pinnacle II Biphenyl Columns (USP L) Physical Characteristics: particle size: 5µm, spherical endcap: yes pore size: 0Å ph range: 2.5 to 7.5 temperature limit: 80 C Inj.: 0µL Conc.: 30µg/mL (30ppm) each Sample diluent: acetonitrile Sample temp.: ambient Standard: Cat. #: 3452, 3453, 3450, 345 Column: Pinnacle II Biphenyl Cat. #: 9209565 Dimensions: 50 x 4.6mm Pore size: 0Å Temp.: water:methanol, 50:50, v/v.5 ml/min. ambient UV @ 254nm. HMX 2. RDX 3. nitrobenzene 4.,2-dinitrobenzene (SS) 5. 4-amino-2,6-dinitrotoluene 6. 2-amino-4,6-dinitrotoluene 7.,3-dinitrobenzene 8. 2-nitrotoluene 9a. 3-nitrotoluene 9b. 4-nitrotoluene 0. 2,6-dinitrotoluene. 3,4-dinitrotoluene (IS) 2.,3,5-trinitrobenzene 3. 2,4-dinitrotoluene 4. tetryl 5. 2,4,6-trinitrotoluene Restek Manufactured Silica Chromatographic Properties: The Pinnacle II Biphenyl phase offers alternate selectivity to straight-chain hydrocarbon phases, and enhanced selectivity and retention for unsaturated compounds, compared to traditional phenyl phases. An excellent confirmation column for explosive compounds, as in EPA method 8330. 4.6mm ID Length cat.# price 5µm Column 50mm 9209565 250mm 9209575 Explosives by US EPA Method 8330 on a Pinnacle II Biphenyl column. Application Page # Explosives...........7, 70 HPLC COLUMNS LC_0322 Pinnacle II Silica Columns (USP L3) Physical Characteristics: Restek Manufactured particle size: 3µm or 5µm, spherical endcap: no Silica pore size: 0Å ph range: 2.5 to 0 carbon load: none temperature limit: 80 C Chromatographic Properties: Good general purpose packing for normal phase separations. Moderate surface area. Replaces Hypersil and Pinnacle Silica..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 92033 920332 920333 920335 50mm 92035 920352 920353 920355 00mm 9203 92032 92033 92035 5µm Columns 30mm 92053 920532 920533 920535 50mm 92055 920552 920553 920555 00mm 9205 92052 92053 92055 50mm 92056 920562 920563 920565 200mm 92052 920522 920523 920525 250mm 92057 920572 920573 920575 Application Page # Hydrocodone Bitartrate....85 Tocopherols.............77

8 Allure C8 and Basix Columns Allure Columns: 3μm or 5μm particles; 60Å pore size Small pore size in a high-purity, Type B silica provides a large surface area. High carbon loads, highly retentive. An excellent choice for light scattering (ELSD) and MS detectors, in which more organic solvent in the mobile phase gives better sensitivity. HPLC COLUMNS Application Page # Antibiotics..............89 Herbicides..............73 Pesticides...............74 also available More chromatograms available! Visit or see our annual product catalog. Allure C8 Columns (USP L) Excellent Columns for LC/MS and ELSD Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 60Å ph range: 2.5 to 7.5 carbon load: 27% temperature limit: 80 C Chromatographic Properties: Most retentive of our alkyl stationary phases due to large surface area of the base silica and high-density bondings. Provides excellent peak shapes for a wide range of compounds..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 96433 964332 964333 964335 50mm 96435 964352 964353 964355 00mm 9643 96432 96433 96435 5µm Columns 30mm 96453 964532 964533 964535 50mm 96455 964552 964553 964555 00mm 9645 96452 96453 96455 50mm 96456 964562 964563 964565 200mm 96452 964522 964523 964525 250mm 96457 964572 964573 964575 Application Page # Acetaminophen/Narcotic Analgesics.............84 Acetaminophen, Pseudoephedrine & Caffeine.............96 Antiarrhythmics.......86, 87 Antidepressants..........89 Albuterol...............9 Herbicides..............72 Ibuprofen & Pseudoephedrine........96 Sedatives...............95 Steroid Hormones........94 Sulfamethoxazole.........89 Allure Basix Columns (USP L0) Excellent Columns for LC/MS and ELSD Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 60Å ph range: 2.5 to 7.5 carbon load: 2% temperature limit: 80 C Chromatographic Properties: Highly retentive propyl cyano phase. Excellent choice for basic compounds and for analytes containing amine group functionality..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 9633 96332 96333 96335 50mm 9635 96352 96353 96355 00mm 963 9632 9633 9635 5µm Columns 30mm 9653 96532 96533 96535 50mm 9655 96552 96553 96555 00mm 965 9652 9653 9655 50mm 9656 96562 96563 96565 200mm 9652 96522 96523 96525 250mm 9657 96572 96573 96575

Allure PFP Propyl and Aqueous C8 Columns 9 Allure PFP Propyl Columns (USP L43) Excellent Columns for LC/MS and ELSD Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 60Å ph range: 2.5 to 7.5 carbon load: 7% temperature limit: 80 C Chromatographic Properties: A pentafluorophenyl phase with a propyl spacer. Highly retentive for basic analytes. An excellent phase for separating nucleosides, nucleotides, purines, pyrimidines, halogenated compounds, β-blockers, and tricyclic antidepressants. Application Page # Catecholamines..........68 Cocaine/Ecgonine Methyl Ester............67 Nucleic Acid Bases.......66 Pesticides...............74.0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 96933 969332 969333 969335 50mm 96935 969352 969353 969355 00mm 9693 96932 96933 96935 5µm Columns 30mm 96953 969532 969533 969535 50mm 96955 969552 969553 969555 00mm 9695 96952 96953 96955 50mm 96956 969562 969563 969565 200mm 96952 969522 969523 969525 250mm 96957 969572 969573 969575 HPLC COLUMNS Allure Aqueous C8 Columns (USP L) Excellent Columns for LC/MS and ELSD Physical Characteristics: particle size: 5µm spherical endcap: no pore size: 60Å ph range: 2.5 to 7.5 temperature limit: 80 C Chromatographic Properties: Highly retentive and selective phase for separating polar analytes, including polar acidic compounds. Compatible with highly aqueous (up to 00%) mobile phases. Highly base deactivated. An excellent choice when analyzing a wide range of compounds, as in LC/MS screening methods..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 5µm Columns 30mm 96853 968532 968533 968535 50mm 96855 968552 968553 968555 00mm 9685 96852 96853 96855 50mm 96856 968562 968563 968565 200mm 96852 968522 968523 968525 250mm 96857 968572 968573 968575 ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: 97435-700 Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39.

20 Allure Biphenyl and Organic Acids Columns HPLC COLUMNS Application Page # Corticosteroids..........92 Steroid Hormones........93 Steroids (Contraceptive)...93 Tetracyclines............90 free literature Allure Biphenyl HPLC Columns Flyer Analyses of corticosteroids, contraceptive steroids, and endogenous steroid hormones show the unique separation mechanism of the Allure Biphenyl phase - pi-pi interactions - provides markedly better selectivity, relative to a C8 or phenyl phase, for molecules that differ in the number or positions of multiple bonds. For saturated analytes the Allure Biphenyl phase has retention and selectivity similar to a C8 phase, but superior to a phenyl phase. Download your free copy from! lit. cat.# 58005 Application Page # Carboxylic Acids..........80 Fruit Juice Acids.........8 Allure Biphenyl Columns (USP L) Physical Characteristics: new! particle size: 3µm or 5µm, spherical endcap: yes pore size: 60Å ph range: 2.5 to 7.5 carbon load: 23% temperature limit: 80 C Chromatographic Properties: Highly retentive and selective for aromatic and unsaturated compounds. Increased retention and selectivity, compared to phenyl phases. Excellent selectivity for steroids, tetracyclines, explosives, and other unsaturated compounds..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 96633 966332 966333 966335 50mm 96635 966352 966353 966355 00mm 9663 96632 96633 96635 5µm Columns 30mm 96653 966532 966533 966535 50mm 96655 966552 966553 966555 00mm 9665 96652 96653 96655 50mm 96656 966562 966563 966565 200mm 96652 966522 966523 966525 250mm 96657 966572 966573 966575 Allure Organic Acids Columns Physical Characteristics: particle size: 5µm, spherical pore size: 60Å Chromatographic Properties: Allure Organic Acids columns provide enhanced retention and selectivity for polar organic acids, allowing the separation to be performed on a single 30cm column. An Allure Organic Acids column effectively resolves key organic acids such as tartaric and quinic acids, using the chromatographic conditions specified in AOAC method 986.3. Retention is stable and reproducible, even with the 00% aqueous mobile phase specified in the AOAC method. Note: Other dimensions available on request. endcap: no ph range: 2.5 to 7.5 temperature limit: 80 C 3.2mm ID 4.6mm ID Length cat.# price cat.# price 5µm Column 50mm 965563 965565 250mm 965575 300mm 965585 ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: 97435-700 Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39.

Allure Silica and AK Columns 2 Allure Silica Columns (USP L3) Physical Characteristics: particle size: 3µm or 5µm, spherical pore size: 60Å endcap: no ph range: 2.5 to 7.5 temperature limit: 80 C Chromatographic Properties: Highly retentive phase for normal phase separations. Very high surface area, Type B silica packing..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 96033 960332 960333 960335 50mm 96035 960352 960353 960355 00mm 9603 96032 96033 96035 5µm Columns 30mm 96053 960532 960533 960535 50mm 96055 960552 960553 960555 00mm 9605 96052 96053 96055 50mm 96056 960562 960563 960565 200mm 96052 960522 960523 960525 250mm 96057 960572 960573 960575 Allure AK Columns Physical Characteristics: particle size: 5µm pore size: 60Å endcap: yes ph range: 2.5-7.5 temperature limit: 80 C new! Chromatographic Properties: This highly retentive, highly selective phase, unique to Restek, was developed specifically for the analysis of aldehydes and ketones as DNPH derivatives. Allure AK is a reversed phase HPLC material that has the unique ability to separate all thirteen carbonyl compounds specified in California Air Resources Board (CARB) Method # 004, using a simple acetonitrile/water gradient, in less than 5 minutes. Other columns require long analysis times or the use of tetrahydrofuran. Application Page # Carbonyls............2, 69 HPLC COLUMNS 3.2mm ID 4.6mm ID Length cat.# price cat.# price 5µm Columns with Trident Integral Inlet Fittings 200mm 959523-700 959525-700 Carbonyls by CARB Method 004 on an Allure AK column. dinitrophenylhydrazine (DNPH) derivatives of aldehydes/ketones Inj.: 0µL Conc.: 3µg/mL each analyte, as aldehyde/ketone Sample diluent: acetonitrile Column: Allure AK Cat.#: 959525-700 Dimensions: 200 x 4.6mm Pore size: 60Å A) water : B):acetonitrile Time (min.) %B 0 60 8 70 0 00.5mL/min. Temp.: 30 C UV @ 360nm Ret. Time Peak (min.) DNPH derivative of:. formaldehyde 4.74 2. acetaldehyde 5.78 3. acrolein 6.86 4. acetone 7.09 5. propionaldehyde 7.3 6. crotonaldehyde 8.9 7. methacrolein 8.55 8. butyraldehyde 8.79 9. methylethylketone 9.06 0. benzaldehyde 0.03. valeraldehyde 0.39 2. m-tolualdehyde.08 3. hexaldehyde.36 Frank Dorman Director of Technical Development + years of service! LC_EV0393

22 Ultra C8 and C8 Columns Ultra Columns: 3μm or 5μm particles; 00Å pore size Our broadest selection of stationary phases, including unique phases. High density bondings, for maximum retention. High-purity, Type B silica gives excellent peak shapes for a wide range of compounds. HPLC COLUMNS Application Page # Acetaminophen/Narcotic Analgesics.............84 Aldehydes/Ketones.......69 Beclomethasone.........88 Corticosteroids..........92 Explosives..............70 Herbicides..............7 Hydrocodone Bitartrate/ Acetaminophen.........96 Nitrofuran Metabolites......83 Vitamins (Fat Soluble).....78 Ultra C8 Columns (USP L) Excellent for a wide range of analyses Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 00Å ph range: 2.5 to 7.5 carbon load: 20% temperature limit: 80 C Chromatographic Properties: A retentive, high-purity packing that exhibits excellent peak shape for a wide range of compounds. Excellent general-purpose reversed phase column..0mm ID 2.mm ID 3.2mm ID 4.0mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 97433 974332 974333 974335 50mm 97435 974352 974353 974355 00mm 9743 97432 97433 97435 5µm Columns 30mm 97453 974532 974533 974535 50mm 97455 974552 974553 974555 00mm 9745 97452 97453 97454 97455 50mm 97456 974562 974563 974564 974565 200mm 97452 974522 974523 974525 250mm 97457 974572 974573 974575 Application Page # Oxycodone..............85 Vanillin & Ethyl Vanillin....82 Heather Johnson Special Events Coordinator 0+ years of service! Ultra C8 Columns (USP L7) Excellent for a wide range of analyses Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 00Å ph range: 2.5 to 7.5 carbon load: 2% temperature limit: 80 C Chromatographic Properties: A retentive, high-purity, base-deactivated reversed phase packing that exhibits excellent peak shape for a wide range of compounds. Less retention for neutral, hydrophobic compounds, compared to the Ultra C8 column..0mm ID 2.mm ID 3.2mm ID 4.0mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 90333 903332 903333 903335 50mm 90335 903352 903353 903355 00mm 9033 90332 90333 90335 5µm Columns 30mm 90353 903532 903533 903535 50mm 90355 903552 903553 903555 00mm 9035 90352 90353 90354 90355 50mm 90356 903562 903563 903564 903565 200mm 90352 903522 903523 903525 250mm 90357 903572 903573 903575

Ultra Aqueous C8 and IBD Columns 23 Ultra Aqueous C8 Columns (USP L) Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: no pore size: 00Å ph range: 2.5 to 7.5 temperature limit: 80 C Chromatographic Properties: Highly retentive and selective for reversed phase separations of polar analytes. Highly base-deactivated. Compatible with highly aqueous (up to 00%) mobile phases..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 97833 978332 978333 978335 50mm 97835 978352 978353 978355 00mm 9783 97832 97833 97835 5µm Columns 30mm 97853 978532 978533 978535 50mm 97855 978552 978553 978555 00mm 9785 97852 97853 97855 50mm 97856 978562 978563 978565 200mm 97852 978522 978523 978525 250mm 97857 978572 978573 978575 Application Page # Amino Acids (Aromatics)...67 Analgesics..............84 Carboxylic Acids..........80 Herbicides...........7, 72 Phenethyl Glucosinolate...77 Sudan Dyes.............82 also available More chromatograms available! Visit or see our annual product catalog. HPLC COLUMNS Ultra IBD Columns Specialized Columns for Mixed Polar and Nonpolar Compounds Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: no pore size: 00Å ph range: 2.5 to 7.5 carbon load: 2% temperature limit: 80 C Chromatographic Properties: An intrinsically base-deactivated (IBD) phase, containing a polar group within, or intrinsic to, the hydrocarbon bonded phase. Unique selectivity and a high level of base deactivation, while reducing or eliminating the need for mobile phase additives..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 97533 975332 975333 975335 50mm 97535 975352 975353 975355 00mm 9753 97532 97533 97535 5µm Columns 30mm 97553 975532 975533 975535 50mm 97555 975552 975553 975555 00mm 9755 97552 97553 97555 50mm 97556 975562 975563 975565 200mm 97552 975522 975523 975525 250mm 97557 975572 975573 975575 Application Page # Antiarrhythmics..........86 Antibiotics...........88, 89 Cimetidine..............9 Diflubenzuron (Pesticide)..7 Glyburide...............9 Nucleosides, Nucleotides & Nucleic Acid Bases.....66 Vitamins (Water Soluble)...78 ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: 97435-700 Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39.

24 Ultra C4 and C Columns Ultra C4 Columns (USP L26) Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 00Å ph range: 2.5 to 7.5 carbon load: 9% temperature limit: 80 C Chromatographic Properties: Exceptionally stable C4 packing, with high bonding coverage and base deactivation. Less retention than C8 or C8 phases. HPLC COLUMNS also available More chromatograms available! Visit or see our annual product catalog..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 90233 902332 902333 902335 50mm 90235 902352 902353 902355 00mm 9023 90232 90233 90235 5µm Columns 30mm 90253 902532 902533 902535 50mm 90255 902552 902553 902555 00mm 9025 90252 90253 90255 50mm 90256 902562 902563 902565 200mm 90252 902522 902523 902525 250mm 90257 902572 902573 902575 Ultra C Columns (USP L3) Physical Characteristics: particle size: 3µm or 5µm, spherical pore size: 00Å ph range: 2.5 to 7.5 carbon load: 5% temperature limit: 80 C Chromatographic Properties: Exceptionally stable C phase. Least retentive reversed phase hydrocarbon packing..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 9033 90332 90333 90335 50mm 9035 90352 90353 90355 00mm 903 9032 9033 9035 5µm Columns 30mm 9053 90532 90533 90535 50mm 9055 90552 90553 90555 00mm 905 9052 9053 9055 50mm 9056 90562 90563 90565 200mm 9052 90522 90523 90525 250mm 9057 90572 90573 90575 ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: 97435-700 Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39.

Ultra Cyano and Phenyl Columns 25 Ultra Cyano Columns (USP L0) Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 00Å ph range: 2.5 to 7.5 carbon load: 8% temperature limit: 80 C Chromatographic Properties: High-purity cyano phase with few silanol sites. Often a better choice than C8 phases for basic pharmaceuticals, especially regarding peak shape and selectivity. Cyano phases are more rugged than bare silica for normal phase analyses because they are less sensitive to small amounts of water in the mobile phase. Application Page # Antiarrhythmics..........85.0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 90633 906332 906333 906335 50mm 90635 906352 906353 906355 00mm 9063 90632 90633 90635 5µm Columns 30mm 90653 906532 906533 906535 50mm 90655 906552 906553 906555 00mm 9065 90652 90653 90655 50mm 90656 906562 906563 906565 200mm 90652 906522 906523 906525 250mm 90657 906572 906573 906575 HPLC COLUMNS Ultra Phenyl Columns (USP L) Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 00Å ph range: 2.5 to 7.5 carbon load: 0% temperature limit: 80 C Chromatographic Properties: High-purity, highly retentive, base-deactivated phase with alternative selectivity to straight chain hydrocarbon phases, especially for aromatic analytes. Application Page # Guaifenesin/Codeine......96.0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 90533 905332 905333 905335 50mm 90535 905352 905353 905355 00mm 9053 90532 90533 90535 5µm Columns 30mm 90553 905532 905533 905535 50mm 90555 905552 905553 905555 00mm 9055 90552 90553 90555 50mm 90556 905562 905563 905565 200mm 90552 905522 905523 905525 250mm 90557 905572 905573 905575 a plus story After the disaster of 9/, Diazald, a highly explosive compound used in herbicide analysis, was immediately controlled by the U.S. government which made shipment impossible. Restek was instrumental in helping me to develop an isocratic HPLC method that did not require the use of Diazald. This method is not only safer, but it saves us time and money. Thanks, Restek! Chris Domaradzki, Organics Manager, Environmental Testing Laboratories Vernon Bartlett HPLC Senior Innovations Chemist 9+ years of service!

26 Ultra Amino and PFP Columns HPLC COLUMNS also available More chromatograms available! Visit or see our annual product catalog. Ultra Amino Columns (USP L8) Physical Characteristics: particle size: 3µm or 5µm, spherical pore size: 00Å ph range: 2.5 to 7.5 carbon load: 2% temperature limit: 80 C Chromatographic Properties: Recommended for normal phase analyses of mono- and disaccharides, or similar compounds..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 90733 907332 907333 907335 50mm 90735 907352 907353 907355 00mm 9073 90732 90733 90735 5µm Columns 30mm 90753 907532 907533 907535 50mm 90755 907552 907553 907555 00mm 9075 90752 90753 90755 50mm 90756 907562 907563 907565 200mm 90752 907522 907523 907525 250mm 90757 907572 907573 907575 Application Page # Digitalis Extract..........86 Levothyroxine............94 Purines/Pyrimidines......67 Randy Romesberg HPLC Senior Innovations Chemist 2+ years of service! Ultra PFP Columns (USP L43) Unique Selectivity Physical Characteristics: particle size: 3µm or 5µm, spherical endcap: fully endcapped pore size: 00Å ph range: 2.5 to 7.5 carbon load: 7% temperature limit: 80 C Chromatographic Properties: A pentafluorophenyl phase. Unique selectivity for compounds containing organohalogens or other basic functional groups..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 97633 976332 976333 976335 50mm 97635 976352 976353 976355 00mm 9763 97632 97633 97635 5µm Columns 30mm 97653 976532 976533 976535 50mm 97655 976552 976553 976555 00mm 9765 97652 97653 97655 50mm 97656 976562 976563 976565 200mm 97652 976522 976523 976525 250mm 97657 976572 976573 976575 ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: 97435-700 Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39.

Ultra Silica and Carbamate Columns 27 Ultra Silica Columns (USP L3) Physical Characteristics: particle size: 3µm or 5µm, spherical pore size: 00Å carbon load: none Chromatographic Properties: High surface area, Type B silica packing. endcap: no ph range: 2.5 to 7.5 temperature limit: 80 C.0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 90033 900332 900333 900335 50mm 90035 900352 900353 900355 00mm 9003 90032 90033 90035 5µm Columns 30mm 90053 900532 900533 900535 50mm 90055 900552 900553 900555 00mm 9005 90052 90053 90055 50mm 90056 900562 900563 900565 200mm 90052 900522 900523 900525 250mm 90057 900572 900573 900575 HPLC COLUMNS Ultra Carbamate Columns Physical Characteristics: particle size: 3µm or 5µm, spherical pore size: 00Å ph range: 2.5 to 7.5 temperature limit: 80 C restek exclusive! Application Page # Pesticides (Carbamates)...73 Chromatographic Properties: Restek chemists developed the Ultra Carbamate column specifically for carbamates analysis. The unique packing separates 0 target carbamates in just over 0 minutes. The column is compatible with fluorescence or LC/MS detection.* An Ultra Carbamate column can process as many as 3 to 4 samples per hour, versus less than 2 samples per hour on a general-purpose C8 column. In addition to increased sample throughput, this much faster analysis will significantly reduce solvent usage and the costs of disposing of solvent waste..0mm ID 2.mm ID 3.2mm ID 4.0mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price cat.# price 3µm Columns 50mm 97735 977352 977353 977354 977355 00mm 9773 97732 97733 97735 5µm Columns 250mm 977575 *For post-column derivatization / fluorescence detection applications for a 4.6mm ID column the total system dead volume, including the post-column reactor, must be less than 650µL. For standard post-column reactor systems, we recommend a 250 x 4.6mm, 5µm column. Call Restek technical service for more information. restek innovation! Faster analyses and reduced solvent use! An Ultra Carbamate column can process as many as 3 to 4 samples per hour, versus less than 2 samples per hour on a general-purpose C8 column. also available We also have syringe filters! See

28 Ultra Quat Columns HPLC COLUMNS restek innovation! An Ultra Quat column and Ultra Quat Reagent Solution eliminate the need for ion pairing reagents in paraquat/diquat analysis. Application Page # Paraquat/Diquat......28, 75 for more info For more information about the Ultra Quat system, review Restek Advantage 2004 vol. 3 at. Ultra Quat Columns Physical Characteristics: particle size: 5µm, spherical pore size: 00Å Ultra Quat Reagent Solution Use with Ultra Quat HPLC column. Dilute to liter mobile phase, per instructions. In water, 20mL/bottle cat. # 3244 (ea.) ph range: 2.5 to 7.5 temperature limit: 80 C Paraquat & Diquat Calibration Mix diquat dibromide paraquat dichloride,000µg/ml each in water, ml/ampul cat. # 32437 (ea.) Consistent resolution, retention times, and peak symmetry for paraquat and diquat on an Ultra Quat column. 2 restek exclusive! Chromatographic Properties: A retentive, high-purity, base deactivated reversed phase packing. Ideal for the analysis of paraquat and diquat or other quaternary amines when used with Ultra Quat Reagent Solution mobile phase additive (cat. # 3244). 4.6mm ID Length cat.# price 5µm Column 50mm 98565 free literature Peak time (min.). diquat 7.5 2. paraquat 8.8 308 nm 257 nm Simple, Sensitive HPLC/UV Analysis for Paraquat and Diquat These highly charged quaternary amines are poorly retained on alkyl stationary phases. Using only acetonitrile, water, and a solvationblocking reagent, our separation system alters the interactions among analyte, mobile phase, and stationary phase, and promotes solubility of the analytes in the stationary phase. In our system, the detection limit is 6ppb for either herbicide, and the analysis is completed in less than 0 minutes. An optimized solid phase extraction cartridge concentrates the herbicides for the analysis. Inj.: 20µL Conc.: 20ppm each component (above); 20µg/mL, 40µg/mL, and 00µg/mL each component at 308nm (left) and at 257nm (right) Sample diluent: water Column: Ultra Quat Cat. #: 98565 Dimensions: 50 x 4.6mm Temp.: 27 C Ultra Quat Reagent Solution (cat.# 3244): acetonitrile, 95:5 (v/v).0 ml/min. UV @ 257nm (paraquat) UV @ 308nm (diquat) 2 min. Call Restek at 800-356-688 or 84-353-300, ext. 5, or contact your Restek representative, to request your free copy! 308 nm 257 nm LC_032 min. lit. cat.# 580006

Viva C8 and C8 Columns 29 Viva Wide Pore HPLC Columns: 3μm or 5μm particles; 300Å pore size Excellent for separating peptides or proteins. Rugged, spherical particles, with 300Å pore size. High proportion of pore/surface area available to large molecules. Viva columns are based on a wide pore material we designed for optimal large molecule separations. In developing Viva silica, we found that although many commercial wide-pore silicas meet the standard 300Å mean pore size, most have very broad distributions about this mean, with a significant portion of their pore volume falling below 50Å. This means a large portion of the surface area is unavailable to larger molecules. Viva columns have a narrow distribution about the mean pore size, permitting a larger portion of the silica surface to play a role in the separation. Viva C8 Columns (USP L) Physical Characteristics: Restek Manufactured particle size: 3µm or 5µm, spherical endcap: yes Silica pore size: 300Å ph range: 2.5 to 0 carbon load: 9% temperature limit: 80 C Chromatographic Properties: Highly base-deactivated wide pore packing that exhibits excellent peak shape for a wide range of compounds. Excellent general-purpose column for analyzing large molecules and biomolecules..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 3µm Columns 30mm 95433 954332 954333 954335 50mm 95435 954352 954353 954355 00mm 9543 95432 95433 95435 50mm 95436 954362 954363 954365 5µm Columns 30mm 95453 954532 954533 954535 50mm 95455 954552 954553 954555 00mm 9545 95452 95453 95455 50mm 95456 954562 954563 954565 200mm 95452 954522 954523 954525 250mm 95457 954572 954573 954575 restek innovation! Viva silica has a narrow distribution about the mean pore size, permitting a larger portion of the silica surface to play a role in the separation of large molecules and biomolecules. Application Page # Peptides................68 Proteins................68 new! ordering note If you need another column dimension or phase, please call for a quote. HPLC COLUMNS Viva C8 Columns (USP L7) Physical Characteristics: Restek Manufactured particle size: 5µm, spherical endcap: yes Silica pore size: 300Å ph range: 2.5 to 7.5 carbon load: 5% temperature limit: 80 C Chromatographic Properties: Highly base-deactivated wide pore packing that exhibits excellent peak shape for a wide range of compounds. Less retention in reversed phase assays than Viva C8..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 5µm Columns 30mm 95353 953532 953533 953535 50mm 95355 953552 953553 953555 00mm 9535 95352 95353 95355 50mm 95356 953562 953563 953565 200mm 95352 953522 953523 953525 250mm 95357 953572 953573 953575 ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: 97435-700 Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39. also available 3µm particles are available for all Viva phases please inquire. Tawnya Childs Call Center Manager 0+ years of service!

30 Viva C4, Biphenyl and PFP Propyl Columns HPLC COLUMNS Tim Herring Technical Service Specialist 3+ years of service! for more info For more information, request our Viva Wide Pore HPLC Columns flyer. lit. cat# 59939 also available 3µm particles are available for all Viva phases please inquire. Viva C4 Columns (USP L26) Physical Characteristics: Restek Manufactured particle size: 5µm, spherical endcap: yes Silica pore size: 300Å ph range: 2.5 to 7.5 carbon load: 3.5% temperature limit: 80 C Chromatographic Properties: Highly base-deactivated wide pore packing that exhibits excellent peak shape for a wide range of compounds. Less retention in reversed phase assays than Viva C8 or Viva C8..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 5µm Columns 30mm 95253 952532 952533 952535 50mm 95255 952552 952553 952555 00mm 9525 95252 95253 95255 50mm 95256 952562 952563 952565 200mm 95252 952522 952523 952525 250mm 95257 952572 952573 952575 new! Viva Biphenyl Columns (USP L) Physical Characteristics: particle size: 5µm pore size: 300Å carbon load: 6.7% endcap: yes ph range: 2.5 to 7.5 temperature limit: 80 C restek exclusive! Chromatographic Properties: Highly base-deactivated wide pore packing that exhibits excellent peak shape for a wide range of compounds; ideal for large molecule and biomolecule assays. Highly retentive and selective phase for aromatic and unsaturated compounds, with increased retention, relative to phenyl phases..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 5µm Columns 30mm 95653 956532 956533 956535 50mm 95655 956552 956553 956555 00mm 9565 95652 95653 95655 50mm 95656 956562 956563 956565 200mm 95652 956522 956523 956525 250mm 95657 956572 956573 956575 new! Viva PFP Propyl Columns (USP L43) Physical Characteristics: particle size: 5µm, spherical pore size: 300Å carbon load: 5% endcap: yes ph range: 2.5 to 7.5 temperature limit: 80 C Restek Manufactured Silica Chromatographic Properties: A pentafluorophenyl phase with a propyl spacer. Highly retentive for basic analytes. Highly base-deactivated wide pore packing that exhibits excellent peak shape for a wide range of compounds, including nucleosides, nucleotides, and halogenated compounds..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 5µm Columns 30mm 95953 959532 959533 959535 50mm 95955 959552 959553 959555 00mm 9595 95952 95953 95955 50mm 95956 959562 959563 959565 200mm 95952 959522 959523 959525 250mm 95957 959572 959573 959575

Viva Silica Columns (USP L3) Physical Characteristics: particle size: 5µm, spherical pore size: 300Å ph range: 2.5 to 7.5 temperature limit: 80 C Viva Silica Columns; Fast LC 3 Restek Manufactured Silica Chromatographic Properties: Highly base-deactivated wide pore packing that exhibits excellent peak shape for a wide range of compounds in normal phase separations..0mm ID 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price cat.# price 5µm Columns 30mm 95053 950532 950533 950535 50mm 95055 950552 950553 950555 00mm 9505 95052 95053 95055 50mm 95056 950562 950563 950565 200mm 95052 950522 950523 950525 250mm 95057 950572 950573 950575 ordering note To order a 2.mm, 3.2mm, or 4.6mm ID column with a Trident Integral Inlet Fitting, add -700 to the catalog number for the column. Nominal additional charge Example: 00mm x 4.6mm ID Ultra C8 column with Trident Integral Inlet Fitting: 97435-700 Also order an XG-XF fitting (cat.#25026 or 25062), see page 37. For guard cartridges for these columns, see page 39. HPLC COLUMNS Fast LC Method Development Kits Fast, efficient separations in an economical cartridge format. The design allows rapid substitution of one cartridge for another. The holder is available with either standard fittings or with a universal PEEK tip fitting. The universal fitting gives the user the option of threading the column directly into the detector inlet, reducing system dwell volume. The cartridges are 30mm long, with either 2.mm or 4.0mm ID. The 2.mm and 4.0mm ID cartridges have the same outside diameter, so the holder will accept either. Each kit includes four 3µm Fast LC Cartridges: Ultra C8 (USP L), Ultra Aqueous C8 (USP L), Ultra Cyano (USP L0), Ultra PFP (USP L43), and a Fast LC cartridge holder. Fast LC Development Kits Description qty. cat.# price Fast LC Development Kit, four 30 x 2.mm columns kit 25296 Fast LC Development Kit, four 30 x 4.0mm columns kit 25297 3μm Fast LC Cartridges Description Length 2.mm ID 4.0mm ID price Ultra C8 Fast LC Cartridge 30mm 9743320 9743340 Ultra Aqueous C8 Fast LC Cartridge 30mm 9783320 9783340 Ultra Cyano Fast LC Cartridge 30mm 9063320 9063340 Ultra PFP Fast LC Cartridge 30mm 9763320 9763340 Fast LC Cartridge Holders Description qty. cat.# price Fast LC Cartridge Holder with PEEK Tip ea. 25298 Fast LC Cartridge Holder with Standard Fittings ea. 25299 Fast LC saves time and conserves solvent. also available Additional column kits and Fast LC cartridges are available. Please call for more information.

32 Kromasil Columns Kromasil Chromatography Products Perfectly spherical, totally porous HPLC silica products from Kromasil. Wide range of bonded phases and particle sizes. Excellent chemical purity and stability, for highly efficient, reproducible separations. Kromasil HPLC silica products consist of highly spherical, porous particles in sizes from 3.5µm to 6µm and larger. The surface properties of the silica have been optimized, and include a narrow pore size distribution and a well-defined pore structure. For chromatographic separations, these properties ensure higher efficiencies, lower pressure drops, and excellent lot-to-lot reproducibility. HPLC COLUMNS Table I Typical chemical purity for Kromasil spherical silicas. Content Metal (ppm) Na <20 Al <0 Fe <0 Values based on AAS or ICP measurements. Kromasil spherical silicas are produced using a sol-gel technique, which yields a mechanically strong particle with a large surface area (330m 2 /g for 00Å silica). Metal impurities are carefully monitored, as trace metals in the silica structure increase the surface acidity and can lead to tailing peaks for basic or chelating compounds. Typical metal content for Kromasil silicas is shown in Table I. Kromasil bonded phases are manufactured using monofunctional silanes, for higher surface coverage and greater reproducibility. Bonding is followed by an end-capping procedure, which further increases chemical stability. Restek offers all Kromasil phases, in a full range of column dimensions and silica particle sizes. A sampling of our product offering is shown here. For further information about Kromasil products available from Restek, please contact Restek technical service at 800-356-688, ext. 4, 84-353-300, ext. 4, or support@restek.com Kromasil HPLC Columns Physical Characteristics: particle size: 3.5µm or 5µm, spherical pore size: 00Å endcap: fully endcapped ph range:.5 to 0 also available For guard cartridges for these columns, see page 39. Kromasil C8 Columns (USP L) average carbon load: 20% 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price 3.5µm Columns 30mm 9204332 9204335 50mm 9204352 9204355 00mm 920432 920435 50mm 9204362 9204365 5µm Columns 30mm 9204532 9204533 9204535 50mm 9204552 9204553 9204555 00mm 920452 920453 920455 50mm 9204562 9204563 9204565 200mm 9204522 9204523 9204525 250mm 9204572 9204573 9204575 Kromasil C4 Columns (USP L26) average carbon load: 8% 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price 5µm Columns 30mm 9202532 9202533 9202535 50mm 9202552 9202553 9202555 00mm 920252 920253 920255 50mm 9202562 9202563 9202565 200mm 9202522 9202523 9202525 250mm 9202572 9202573 9202575

Kromasil Columns 33 Kromasil C8 Columns (USP L7) average carbon load: 2% 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price 3.5µm Columns 30mm 9203332 9203335 50mm 9203352 9203355 00mm 920332 920335 50mm 9203362 9203365 5µm Columns 30mm 9203532 9203533 9203535 50mm 9203552 9203553 9203555 00mm 920352 920353 920355 50mm 9203562 9203563 9203565 200mm 9203522 9203523 9203525 250mm 9203572 9203573 9203575 Kromasil C Columns (USP L3) 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price 5µm Columns 30mm 920532 920533 920535 50mm 920552 920553 920555 00mm 92052 92053 92055 50mm 920562 920563 920565 200mm 920522 920523 920525 250mm 920572 920573 920575 Kromasil Phenyl Columns (USP L) average carbon load: 4% 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price 5µm Columns 50mm 9205552 9205553 9205555 50mm 9205562 9205563 9205565 250mm 9205572 9205573 9205575 Sean Nolan Materials Manager 7+ years of service! also available Other Kromasil column dimensions, phases, and particle sizes are available. Please contact Technical Service at 800-356-688 or 84-353-300, ext. 4, to inquire. HPLC COLUMNS Kromasil Amino Columns (USP L8) nitrogen load:.7% 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price 5µm Columns 50mm 9207552 9207553 9207555 50mm 9207562 9207563 9207565 250mm 9207572 9207573 9207575 Kromasil Silica Columns (USP L3) 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price 5µm Columns 30mm 9200532 9200533 9200535 50mm 9200552 9200553 9200555 00mm 920052 920053 920055 50mm 9200562 9200563 9200565 200mm 9200522 9200523 9200525 250mm 9200572 9200573 9200575 free literature Kromasil Columns & Bulk Packings Call Restek at 800-356-688 or 84-353-300, ext. 5, or contact your Restek representative, to request your free copy! lit. cat.# 580089

34 Prep Columns HPLC Prep Columns Easy scale-up from Restek analytical columns. Popular bonded phases. Restek makes it easy to scale up your separations. We offer a wide range of semi-preparative and preparative-scale columns packed with many of our popular Ultra, Allure, Pinnacle DB, and Pinnacle II bonded phases on a 5µm spherical silica. Other stationary phases and particle sizes are available if you need a phase on 7µm, 0µm or 5µm particles, please call. HPLC COLUMNS Supersize without surprise! ordering note We strongly recommend ordering a semi-prep or prep column only after scouting the desired separation on an equivalent analytical-scale column. Because we cannot re-use a column or the silica it contains once it has left our facility, we cannot accept returns of large-scale columns (except in cases of our error). Pinnacle DB HPLC Prep Columns Dimensions Pinnacle DB C8 Pinnacle DB C8 Pinnacle DB Cyano Pinnacle DB Silica Length x ID cat.# cat.# cat.# cat.# price 50 x 0mm 944557 943557 946557 940557 50 x 2.2mm 944558 943558 946558 940558 50 x 30mm 944559 943559 946559 940559 50 x 50mm 944550 943550 946550 940550 00 x 0mm 94457 94357 94657 94057 00 x 2.2mm 94458 94358 94658 94058 00 x 30mm 94459 94359 94659 94059 00 x 50mm 94450 94350 94650 94050 50 x 0mm 944567 943567 946567 940567 50 x 2.2mm 944568 943568 946568 940568 50 x 30mm 944569 943569 946569 940569 50 x 50mm 944560 943560 946560 940560 250 x 0mm 944577 943577 946577 940577 250 x 2.2mm 944578 943578 946578 940578 250 x 30mm 944579 943579 946579 940579 250 x 50mm 944570 943570 946570 940570 Pinnacle II HPLC Prep Columns Dimensions Pinnacle II C8 Pinnacle II C8 Pinnacle II Cyano Pinnacle II Silica Length x ID cat.# cat.# cat.# cat.# price 50 x 0mm 924557 923557 926557 920557 50 x 2.2mm 924558 923558 926558 920558 50 x 30mm 924559 923559 926559 920559 50 x 50mm 924550 923550 926550 920550 00 x 0mm 92457 92357 92657 92057 00 x 2.2mm 92458 92358 92658 92058 00 x 30mm 92459 92359 92659 92059 00 x 50mm 92450 92350 92650 92050 50 x 0mm 924567 923567 926567 920567 50 x 2.2mm 924568 923568 926568 920568 50 x 30mm 924569 923569 926569 920569 50 x 50mm 924560 923560 926560 920560 250 x 0mm 924577 923577 926577 920577 250 x 2.2mm 924578 923578 926578 920578 250 x 30mm 924579 923579 926579 920579 250 x 50mm 924570 923570 926570 920570

Prep Columns 35 Allure HPLC Prep Columns Dimensions Allure C8 Allure Basix Allure PFP Propyl Allure Silica Length x ID cat.# cat.# cat.# cat.# price 50 x 0mm 964557 96557 960557 50 x 2.2mm 964558 96558 960558 50 x 30mm 964559 96559 960559 50 x 50mm 964550 96550 960550 00 x 0mm 96457 9657 96057 00 x 2.2mm 96458 9658 96058 00 x 30mm 96459 9659 96059 00 x 50mm 96450 9650 96050 50 x 0mm 964567 96567 960567 50 x 2.2mm 964568 96568 969568 960568 50 x 30mm 964569 96569 969569 960569 50 x 50mm 964560 96560 960560 250 x 0mm 964577 96577 960577 250 x 2.2mm 964578 96578 960578 250 x 30mm 964579 96579 960579 250 x 50mm 964570 96570 960570 Ultra HPLC Prep Columns HPLC COLUMNS Dimensions Ultra C8 Ultra Aqueous C8 Ultra C8 Ultra IBD Ultra Cyano Ultra Phenyl Ultra PFP Ultra Silica Length x ID cat.# cat.# cat.# cat.# cat.# cat.# cat.# cat.# price 50 x 0mm 974557 978557 903557 975557 906557 905557 976557 900557 50 x 2.2mm 974558 978558 903558 975558 906558 905558 976558 900558 50 x 30mm 974559 978559 903559 975559 906559 905559 976559 900559 50 x 50mm 974550 978550 903550 975550 906550 905550 976550 900550 00 x 0mm 97457 97857 90357 97557 90657 90557 97657 90057 00 x 2.2mm 97458 97858 90358 97558 90658 90558 97658 90058 00 x 30mm 97459 97859 90359 97559 90659 90559 97659 90059 00 x 50mm 97450 97850 90350 97550 90650 90550 97650 90050 50 x 0mm 974567 978567 903567 975567 906567 905567 976567 900567 50 x 2.2mm 974568 978568 903568 975568 906568 905568 976568 900568 50 x 30mm 974569 978569 903569 975569 906569 905569 976569 900569 50 x 50mm 974560 978560 903560 975560 906560 905560 976560 900560 250 x 0mm 974577 978577 903577 975577 906577 905577 976577 900577 250 x 2.2mm 974578 978578 903578 975578 906578 905578 976578 900578 250 x 30mm 974579 978579 903579 975579 906579 905579 976579 900579 250 x 50mm 974570 978570 903570 975570 906570 905570 976570 900570 ordering note We strongly recommend ordering a semi-prep or prep column only after scouting the desired separation on an equivalent analytical-scale column. Because we cannot re-use a column or the silica it contains once it has left our facility, we cannot accept returns of large-scale columns (except in cases of our error). Kromasil Prep Columns Dimensions Kromasil C8 Kromasil C8 Length x ID cat.# cat.# price 50 x 0mm 9204557 9203557 50 x 2.2mm 9204558 9203558 50 x 30mm 9204559 9203559 50 x 0mm 9204567 9203567 50 x 2.2mm 9204568 9203568 50 x 30mm 9204569 9203569 250 x 0mm 9204577 9203577 250 x 2.2mm 9204578 9203578 250 x 30mm 9204579 9203579 new!

36 Bulk Packing Materials Bulk Packing Materials Use our bulk packing materials to pack your own columns! Restek is among the small group of column manufacturers capable of producing their own high purity silica. We offer our Pinnacle II and Pinnacle DB silica and bonded phases in bulk. Pinnacle II is an excellent replacement for Hypersil silica. Pinnacle DB is a highly base-deactivated material for analyses of basic compounds and an excellent alternative to Hypersil BDS silica. Bonded phases from our Ultra, Allure, and Viva lines also are available in bulk. Our extensive QC program ensures the high quality and reproducibility of these silicas. Each lot of material is tested for mean particle size and distribution, pore diameter, surface area, and total metals analysis. You can be confident that you are getting consistent, high quality product. HPLC COLUMNS also available Other stationary phases and particle sizes are available; please inquire. Use these materials for easy scale up to preparative scale chromatography, or for packing your own columns. Bulk Packing Materials Description min. qty. cat.# 5-99 grams 00-499 grams 500-999 grams 000 grams 3µm Pinnacle II Bulk Packing Materials Pinnacle II C8 Bulk Packing 5g 9233 /gram /gram /gram /gram Pinnacle II C8 Bulk Packing 5g 9243 /gram /gram /gram /gram Pinnacle II Cyano Bulk Packing 5g 9263 /gram /gram /gram /gram Pinnacle II Phenyl Bulk Packing 5g 9253 /gram /gram /gram /gram 5µm Pinnacle II Bulk Packing Materials Pinnacle II Amino Bulk Packing 5g 9275 /gram /gram /gram /gram Pinnacle II C8 Bulk Packing 5g 9235 /gram /gram /gram /gram Pinnacle II C8 Bulk Packing 5g 9245 /gram /gram /gram /gram Pinnacle II Cyano Bulk Packing 5g 9265 /gram /gram /gram /gram Pinnacle II Phenyl Bulk Packing 5g 9255 /gram /gram /gram /gram 5µm Pinnacle DB Bulk Packing Materials Pinnacle DB C8 Bulk Packing 5g 9445 /gram /gram /gram /gram Pinnacle DB C8 Bulk Packing 5g 9435 /gram /gram /gram /gram Pinnacle DB Cyano Bulk Packing 5g 9465 /gram /gram /gram /gram Description qty. cat.# -9 bottles 0-49 bottles 50-99 bottles 00 bottles 5µm Ultra Bulk Packing Materials Ultra C Bulk Packing 0g/btl. 905 /btl. /btl. /btl. /btl. Ultra C4 Bulk Packing 0g/btl. 9025 /btl. /btl. /btl. /btl. Ultra C8 Bulk Packing 0g/btl. 9035 /btl. /btl. /btl. /btl. Ultra C8 Bulk Packing 0g/btl. 9745 /btl. /btl. /btl. /btl. Ultra Amino Bulk Packing 0g/btl. 9075 /btl. /btl. /btl. /btl. Ultra Cyano Bulk Packing 0g/btl. 9065 /btl. /btl. /btl. /btl. Ultra Phenyl Bulk Packing 0g/btl. 9055 /btl. /btl. /btl. /btl. new! Silica Bulk Materials Description min. qty. cat.# 5-99 grams 00-499 grams 500-999 grams 000 grams Silica Bulk Materials Pinnacle II Silica Bulk Packing, 3µm 5g 9203 /gram /gram /gram inquire/gram Pinnacle II Silica Bulk Packing, 5µm 5g 9205 /gram /gram /gram /gram Pinnacle DB Silica Bulk Packing, 5µm 5g 9405 /gram /gram /gram /gram qty. cat.# -9 bottles 0-49 bottles 50-99 bottles 00 bottles Ultra Silica Bulk Packing, 5µm 0g/btl. 9005 /btl. /btl. /btl. /btl. Kromasil Bulk Packings High-purity packing materials in 0 and 6µm. All Kromasil phases available, please inquire. Description min. qty. cat.# 200-499 grams 500-999 grams 000 grams Kromasil 00Å Silica, 0µm 200g 92000 /gram /gram /gram Kromasil 00Å Silica, 6µm 200g 9200 /gram /gram /gram Kromasil 00Å C8, 0µm 200g 92030 /gram /gram /gram Kromasil 00Å C8, 6µm 200g 9203 /gram /gram /gram Kromasil 00Å C8, 0µm 200g 92040 /gram /gram /gram Kromasil 00Å C8, 6µm 200g 9204 /gram /gram /gram Kromasil 00Å Chiral DMB, 0µm 200g 92080 /gram /gram /gram Kromasil 00Å Chiral DMB, 6µm 200g 9208 /gram /gram /gram Kromasil 00Å Chiral TBB, 0µm 200g 9990 /gram /gram /gram Kromasil 00Å Chiral TBB, 6µm 200g 999 /gram /gram /gram

Restek s Exclusive Trident Integral System Convenient and economical leak-free guard cartridge system, extremely easy to install. Versatile configuration protects against all levels of contamination. Integral design eliminates troublesome tubing connections. Trident Integral Guard Cartridge System 37 The system s foundation consists of the analytical column configured with our exclusive Trident end fitting and XF fitting. This configuration contains the standard internal frit as well as a replaceable cap frit, which easily can be changed without disturbing the packed bed. Changing the external frit can reverse the effects of accumulated particles, such as high backpressure or peak distortion. To obtain this basic configuration, simply order any Restek HPLC column, and add the suffix -700 to the catalog number for the column. (Nominal additional charge. ) Lori McGarry Environmental Health & Safety Officer + year of service! For maximum protection against contaminants and particulate matter, the system can be configured with an integral guard cartridge holder (XG-XF), a guard cartridge, and a replaceable external frit. To obtain this configuration, simply order any Restek HPLC column, add the suffix -700 to the catalog number for the column, and order the appropriate XG-XF male fitting (cat.# 25026 or 25062, below) and Trident guard cartridges (page 39). Description qty. cat.# price XG-XF Fitting for 0mm Guard Cartridge ea. 25026 XG-XF Fitting for 20mm Guard Cartridge ea. 25062 Replacement XF Filter Fitting ea. 25024 Replacement Cap Frits: 4mm, 2.0µm 5-pk. 25022 Replacement Cap Frits: 4mm, 0.5µm 5-pk. 25023 Replacement Cap Frits: 2mm, 2.0µm 5-pk. 25057 HPLC COLUMNS Trident Integral Guard System Assembled column with Trident integral guard system XF End Fitting Cap Frit XG-XF Fitting Guard Cartridge End Fitting Column with Trident Integral Inlet Fitting (to order add -700 to catalog number of column) and guard cartridge, XG-XF fitting, cap frit, and XF end fitting. Remove the XF end fitting and install the guard cartridge in the end of the column. Add the XG-XF fitting (order cat.#25026 for 0mm guard cartidges, cat.#25062 for 20mm guard cartridges). Re-install the XF end fitting with cap frit. The cap frit can be easily replaced if it becomes contaminated/plugged.

38 Trident Direct Guard Cartridge System / Cartridge Holders Trident Direct provides three levels of protection Trident Direct Guard Cartridge System Easy to Use, Low Dead Volume The Ultimate Combination of Convenience and Column Protection Unlike one size fits all guard systems, the Trident Direct system gives you the power to select the right level of protection for your analysis. The system offers three levels of protection and guard cartridges in four dimensions, with a variety of bonded phases to match your analytical column. The economical, leakfree cartridge design provides an unprecedented combination of convenience, economy, and reliability. The foundation of the Trident Direct system is a reusable direct connect holder that easily attaches to any HPLC column using CPI- or Waters-style end fittings.* The system is available in configurations to match different protection level needs: in-line filter, in-line filter with holder for 0mm guard cartridge, and in-line filter with holder for 20mm guard cartridge. The guard cartridges are available in 2. and 4.0mm ID and are interchangeable within the appropriate length holder. HPLC COLUMNS Trident Direct high-pressure filter Protection against particulate matter. Trident Direct 0mm guard cartridge holder with filter Protection against particulate matter and moderate protection against irreversibly adsorbed compounds. *The standard PEEK tip in Trident Direct systems is compatible with Parker, Upchurch, Valco, and other CPI-style fittings. To use Trident Direct systems with Waters-style end fittings, replace the tip with cat.# 25088. Description qty. cat.# price High-pressure filter ea. 25082 0mm guard cartridge holder without filter ea. 25083 0mm guard cartridge holder with filter ea. 25084 20mm guard cartridge holder without filter ea. 25085 20mm guard cartridge holder with filter ea. 25086 Connection tip for Waters-style end fittings ea. 25088 PEEK tip standard fittings ea. 25087 Replacement Cap Frits: 4mm, 2.0µm 5-pk. 25022 Replacement Cap Frits: 4mm, 0.5µm 5-pk. 25023 Replacement Cap Frits: 2mm, 2.0µm 5-pk. 25057 Trident Direct 20mm guard cartridge holder with filter Protection against particulate matter and maximum protection against irreversibly adsorbed compounds. Cap frits Trident HPLC In-Line Guard Cartridge Holders A Trident in-line guard cartridge holder can be used with almost any HPLC column by connecting it with a short piece of /6" tubing, appropriate nuts and ferrules, or finger-tight fittings. The system can be used with Restek columns, or with columns from other manufacturers. Holders are available for either 0mm or 20mm guard cartridges. Either size can be purchased with or without a prefilter, which provides added protection against the particles that can shorten the lifetime of the guard cartridge. Description qty. cat.# price Holder for 0mm guard cartridge ea. 2502 Holder with filter for 0mm guard cartridge ea. 25040 Holder for 20mm guard cartridge ea. 2506 Holder with filter for 20mm guard cartridge ea. 25060 Replacement Cap Frits: 4mm, 2.0µm* 5-pk. 25022 Replacement Cap Frits: 4mm, 0.5µm 5-pk. 25023 Replacement Cap Frits: 2mm, 2.0µm* 5-pk. 25057 *Standard porosity. Holder for 0mm guard cartridge Holder with filter for 0mm guard cartridge Holder for 20mm guard cartridge Holder with filter for 20mm guard cartridge

Trident Guard Cartridges 39 Trident HPLC Guard Cartridges 3-pk. 3-pk. 2-pk. 2-pk. Guard Cartridges (0 x 2.mm) (0 x 4.0mm) (20 x 2.mm) (20 x 4.0mm) price Allure AK new! 9595020 Allure Basix 965022 965020 9650222 9650220 Allure C8 9645022 9645020 96450222 96450220 Allure PFP Propyl 9695022 9695020 96950222 96950220 Allure Silica 9605022 9605020 96050222 96050220 Allure Organic Acids 9655022 9655020 96550222 96550220 Allure Aqueous C8 9685022 9685020 96850222 96850220 Allure Biphenyl 9665022 9665020 96650222 96650220 phidelity C8 95793022 95793020 957930222 957930220 Pinnacle II Amino 9275022 9275020 92750222 92750220 Pinnacle II C8 9235022 9235020 92350222 92350220 Pinnacle II C8 9245022 9245020 92450222 92450220 Pinnacle II Cyano 9265022 9265020 92650222 92650220 Pinnacle II PAH 9295022 9295020 92950222 92950220 Pinnacle II Phenyl 9255022 9255020 92550222 92550220 Pinnacle II Biphenyl 92095020 920950220 Pinnacle II Silica 9205022 9205020 92050222 92050220 Pinnacle DB C8 9435022 9435020 94350222 94350220 Pinnacle DB C8 9445022 9445020 94450222 94450220 Pinnacle DB Cyano 9465022 9465020 94650222 94650220 Pinnacle DB Phenyl 9455022 9455020 94550222 94550220 Pinnacle DB Silica 9405022 9405020 94050222 94050220 Ultra Amino 9075022 9075020 90750222 90750220 Ultra Aqueous C8 9785022 9785020 97850222 97850220 Ultra C 905022 905020 9050222 9050220 Ultra C4 9025022 9025020 90250222 90250220 Ultra C8 9035022 9035020 90350222 90350220 Ultra C8 9745022 9745020 97450222 97450220 Ultra Carbamate 9775022 9775020 97750222 97750220 Ultra Cyano 9065022 9065020 90650222 90650220 Ultra IBD 9755022 9755020 97550222 97550220 Ultra PFP 9765022 9765020 97650222 97650220 Ultra Phenyl 9055022 9055020 90550222 90550220 Ultra Silica 9005022 9005020 90050222 90050220 Ultra Quat 985022 985020 9850222 9850220 Viva C8 9545022 9545020 95450222 95450220 Viva C8 9535022 9535020 95350222 95350220 Viva C4 9525022 9525020 95250222 95250220 Viva PFP Propyl new! 9595022 9595020 95950222 95950220 Viva Biphenyl new! 9565022 9565020 95650222 95650220 Viva Silica 9505022 9505020 95050222 95050220 Kromasil C 9205022 9205020 92050220 Kromasil C4 92025022 92025020 920250220 Kromasil C8 92035022 92035020 920350220 Kromasil C8 92045022 92045020 920450220 Kromasil Amino 92075022 92075020 920750222 920750220 Kromasil Phenyl 92055022 92055020 920550222 920550220 Kromasil Silica 92005022 92005020 920050220 0 & 20mm Guard Cartridges HPLC COLUMNS

40 Replacement Parts HPLC ACCESSORIES Outlet Ball Valve Needle Seat Assembly new! Lamp, VWD G34A Restek Replacement Parts for Agilent HPLC Systems Description Model # Similar to Agilent part # qty. cat.# price Preventive Maintenance Kit (Includes: rotor seal, needle seat, needle assembly, seat cap) 050 0078-6872 kit 25259 Autosampler Preventive Maintenance Kit (Includes: rotor seal, needle assembly, needle seat) 00 G33-68709 kit 2527 Pump Maintenance Kit (Includes: PTFE frit, outlet cap, active inlet cartridge, gold disk seal, 2 piston seals, glass solvent filter) 050 & 00 G3-6870 kit 25270 Outlet Ball Valve, Binary Pump 00 G32-6002 ea. 25267 Outlet Ball Valve 050 & 00 G3-6002 ea. 25276 Sieves for Outlet Valve 050 & 00 5063-6505 0-pk. 25266 Check Valve Cartridge Assembly 090 79835-670 ea. 25344 Piston Seals, Teflon w/graphite 050 & 00 5063-6589 2-pk. 22482 Piston Seals, Teflon w/graphite 050 & 00 5063-6589 0-pk. 22483 Piston Seals (Black) 090 5062-2494 4-pk. 25347 Seal Wash Kit, Binary Pump (4 seals, 4 gaskets) 00 kit 25268 Seal Wash Kit (2 seals, 2 gaskets) 00 kit 25269 Wash Seal 050 & 00 0905-75 ea. 25277 Sapphire Piston 050 & 00 5063-6586 ea. 25273 Sapphire Piston 090 6980-0672 ea. 25345 Needle Seat 050 79846-670 ea. 25258 Needle Seat 090 79846-670 ea. 25348 Needle Seat Assembly 00 G33-870 ea. 25265 Needle Assembly 00 G33-8720 ea. 25278 Rotor Seal (not for use with 725 injection valve) 050 00-0626 ea. 25272 Rotor Seal 00 000-853 ea. 25275 Rotor Seal (Rheodyne -style) 090 00-0623 ea. 25349 Frits, PTFE 050 & 00 008-22707 5-pk. 25466 Seal, Gold Disk (outlet) 050 & 00 500-3707 ea. 25467 Outlet Cap 050 & 00 5062-2485 4-pk. 2539 Outlet Cap & Gold Seal Assembly 050 & 00 2-pk. 2540 Connecting Tube 050 & 00 G3-67304 ea. 25058 Bottle Head Assembly G3-60003 ea. 25059 Detector Lamp, 090 DA, 050 VW/DA/MWD 090, 050 79883-60002 ea. 25260 Lamp, DAD G35A, G365A 00 240-0590 ea. 2526 Lamp, VWD G34A 00 G34-6000 ea. 25262 8453 Deuterium Lamp 240-0605 ea. 25263 G32 Fluorescence Detector Flash Lamp 240-0600 ea. 25264 Lamp, DAD Long Life Deuterium (2000 hours) 00 58-530 ea. 25399 new! Capillary SS Tubing with fittings Mixing Capillary Assembly Capillary Stainless Steel Tubing Assemblies for Agilent HPLC Systems Description Model # Similar to Agilent part # qty. cat.# price Capillary SS Tubing, 30mm x 0.7mm ID, with fittings 090 0090-87305 ea. 26525 Capillary SS Tubing, 800mm x 0.7mm ID, with fittings 050 0078-87305 ea. 26526 Capillary SS Tubing, 80mm x 0.7mm ID, with fittings 00 G33-87305 ea. 26527 Capillary SS Tubing, 700mm x 0.25mm ID, with fittings 050 008-67305 ea. 26528 Capillary SS Tubing, 700mm x 0.25mm ID, with fittings 050 0078-87306 ea. 26529 Seat Capillary, SS Tubing, 0.7mm ID 050 0078-87303 ea. 26530 Capillary SS Tubing, 05mm x 0.7mmID 502-86 ea. 2653 Mixing Capillary Assembly 00 G32-67302 ea. 26532 Capillary SS Tubing, Valve to Metering Head 00 G33-8730 ea. 26533 Capillary SS Tubing, 50mm x 0.7mm ID 502-87 ea. 26534 Capillary SS Tubing, 280mm x 0.7mm ID 502-88 ea. 26535 Capillary SS Tubing, 400mm x 0.7mm ID 502-89 ea. 26536 /6" SS Fitting, Front and Back Ferrules 5062-248 0-pk. 26537

Outlet Cap and Gold Seal Assembly Tool for Agilent 050 & 00 HPLC Systems Easily install the gold seal into the outlet cap. Replacement Parts 4 new! Put Outlet Cap on male part of Assembly Tool. Push the Assembly Tool together. Hold onto Outlet Cap and pull Assembly Tool apart. Put Gold Seal over pin on male part of Assembly Tool. Restek Replacement Parts for Beckman HPLC Systems Push the Assembly Tool together and press the Gold Seal in the Outlet Cap. Pull the Assembly Tool apart and remove assembled Outlet Cap and Gold Seal. Description qty. cat.# price Outlet Cap and Gold Seal Assembly Tool for Agilent 050 & 00 HPLC Systems ea. 24989 Description Model # Similar to Beckman part # qty. cat.# price Inlet Check Valve Assembly 0A&B, 2, 4M, 6, 8, 25, 26, 27, 28 Pumps 240720 ea. 25439 Outlet Check Valve Assembly 0A&B, 2, 4M, 6, 8, 25, 26, 27, 28 Pumps 24072 ea. 25440 Inlet Check Valve Cartridge for Check Valve Assembly 0A&B, 2, 4M, 6, 8, 25, 26, 27, 28 Pumps 240620 ea. 2544 Outlet Check Valve Cartridge for Check Valve Assembly 0A&B, 2, 4M, 6, 8, 25, 26, 27, 28 Pumps 24062 ea. 25442 Guide Sleeve for Graphite Plunger Guide 0 Series Pumps 24373 ea. 25444 Outlet Check Filter Frit Assembly 0 Series Pumps 24069 ea. 25445 Piston Guide Assembly 0 Series and 2 Pumps 243045 ea. 25446 Plunger 0 Series and 2 Pumps 243053 ea. 25447 Plunger Seal 0 Series Pumps 88738 ea. 25448 Plunger Seal 2 Pumps 236797 ea. 25449 Sapphire Plunger 4M, 6, 8, 25, 26, 27, 28 Pumps 24074 ea. 25450 Plunger Seal 4M, 6, 8, 26, 27, 28 Pumps 24037 ea. 2545 Pump Seal, Gold* 4, 6, 25, 26, 27, 28 Pumps 24037 ea. 25452 Plunger Wash Seal 25, 26, 27, 28 Pumps 728772 ea. 25453 Deuterium Lamp DU60, 62, 64, 65 59679 ea. 25454 *Ultra-high molecular weight polyethylene (UHMWPE). Outlet Check Valve Assembly Sapphire Plunger HPLC ACCESSORIES Restek Replacement Parts for Hitachi HPLC Systems Description Model # Similar to Hitachi part # qty. cat.# price SS Check Valve Cartridges ( /6" Ruby Ball) 655/6000/6200 AN0-085 2-pk. 25455 Inlet Check Valve Assembly 655/6000/6200 AN0-0833 ea. 25456 Outlet Check Valve Assembly 655/6000/6200 AN0-0834 ea. 25457 Plunger Assembly 655, 6000, 6200, 700 80-033 ea. 25458 Plunger Seal L655A, 6000, 6200, 700 655-080 ea. 25459 Plunger Seal, Gold 655, 6000, 6200, 700 655-080 ea. 25460 Inlet Check Valve Assembly L-700 AN0-0836 ea. 2546 Outlet Check Valve Assembly L-700 AN0-0837 ea. 25462 Rotor Assembly for Dilutor Valve AS-7200 Autosampler 80-3085 ea. 25463 Rotor Seal Kit AS-7200, AS-7250 Injection Valve AN0-088 ea. 25464 Deuterium Lamp, Prealigned L4000, L4200, L4250, L7400 885-3570 ea. 25465

42 Replacement Parts HPLC ACCESSORIES new! Restek Replacement Parts for PerkinElmer HPLC Systems Description Model # Similar to PerkinElmer part # qty. cat.# price Inlet Check Valve PE 200, 4, 250, 400, 40, 620 254077 ea. 25438 Outlet Check Valve PE 200, 4, 250, 400, 40, 620 254097 ea. 25437 High Pressure Seal Kit PE Series 200 Pumps--, 2, 3, 3B, 4, 0, 250, 400, 40, 620, Int. 4000 Pump 2540275 ea. 25433 Standard High Pressure Seal PE Series 200 Pumps--, 2, 3, 3B, 4, 0, 250, 400, 40, 620, Int. 4000 Pump 9907324 ea. 25434 Low Pressure Seal PE Series 200 Pumps 9907339 ea. 25435 Needle PE Series 200 Pumps N2930023 ea. 25432 Deuterium Lamp PE Lambda--2, 5, 7, 8, 0,, 2, 4, 5, 6, 7, 8, 9, 20, 40, 800, 900 B06097 ea. 25436 Deuterium Lamp PE 200/785A N292049 ea. 2543 Restek Replacement Parts for Shimadzu HPLC Systems Description Model # Similar to Shimadzu part # qty. cat.# price Inlet Check Valve LC-6A, LC-0AS 228-2353-9 ea. 25287 Inlet Check Valve LC-600, LC-9A, LC-0AD 228-8522-9 ea. 25295 Inlet Check Valve LC-0ADvp 228-39093-92 ea. 24984 Inlet Check Valve LC-0AT, LC-0ATvp 228-3266-9 ea. 2652 Outlet Check Valve LC-6A, LC-0AS 228-09054-93 ea. 25288 Outlet Check Valve Rebuild Kit LC-6A, LC-0AS 228-200-9 2-pk. 25289 Outlet Check Valve LC-600, LC-9A, LC-0AD, LC-0AT 228-8522-92 ea. 25282 Outlet Check Valve LC-0ADvp, LC-0ATvp 228-34976-9 ea. 24983 Plunger Seal LC-6A 228-999-00 ea. 25285 Plunger Seal, Polyethylene LC-0AS, LC-0AT, LC-0ATvp 228-2975-00 ea. 25290 Plunger Seal LC-600, LC-9A, LC-0AD 228-8745-00 ea. 25293 Plunger Seal LC-0ADvp 228-3546-00 ea. 24980 Plunger Seal, Gold LC-0ADvp 228-32628-00 ea. 2498 Plunger Seal SIL-0ADvp, LC-0ATvp 228-3545-00 ea. 24985 Plunger Rinse Seal LC-0AS 228-28499-00 ea. 25292 Sapphire Plunger LC-6A 228-2904-93 ea. 25286 Sapphire Plunger LC-0AS 228-709-93 ea. 2529 Sapphire Plunger LC-600, LC-9A, LC-0AD 228-8523-9 ea. 25294 Needle Seal SIL-0A, 0XL, 0ADvp 228-33355-04 ea. 25468 Rotor Seal SIL-0ADvp 228-227-97 ea. 24986 Rotor Seal Assembly SIL-0A, 0AXL 228-227-9 ea. 25469 Stator Assembly SIL-0A, 0AXL 228-2220-9 ea. 25470 Syringe, 500µL SIL-0A, 0AXL 228-25237-04 ea. 2547 Plunger Assembly, Ceramic LC-0ADvp 228-3560-9 ea. 25472 Plunger Assembly, Ceramic LC-0ATvp 228-35009-92 ea. 25473 Plunger Assembly, Sapphire LC-0ADvp 228-3560-92 ea. 24982 Deuterium Lamp SPD-6A 062-65056-02 ea. 25283 Deuterium Lamp SPD-0A, 0AV, M20A 228-3406-02 ea. 25284 Julie Kowalski Innovations Chemist 2+ years of service!

Replacement Parts 43 Restek Replacement Parts for Thermo Separation HPLC Systems Description Model # Similar to SP/TSP part # qty. cat.# price Inlet Check Valve Assembly SP8800 & P-Series Pumps A3495-00 ea. 25474 Outlet Check Valve Assembly SP8800 Series Pumps A3490-00 ea. 25475 Piston SP8800 & P-Series Pumps A302-00 ea. 25476 Back-up Seal SP8800 & P-Series Pumps A2963-00 ea. 25477 Plunger Seal, Gold Superseal SP8800 & P-Series Pumps A2962-00 ea. 25478 Check Valve and Transducer Assembly P-Series Pumps A3990-00 ea. 25479 Kel-F Washer P-Series Pumps A2973-00 ea. 25480 TSP AS00, 300, 000, 3000, 3500, Rotor Seal Assembly, Rheodyne 700 8875, and 8880 Autosamplers 700-039 ea. 2548 TSP AS00, 300, 000, 3000, 3500, Syringe Assembly, 250µL 8875, and 8880 Autosamplers A3588-020 ea. 25482 Syringe, 500µL 8800 Series 330-000 ea. 25483 Lamp, UV Linear UV-200, 203, 204, 205, 206, and UV 00, 50, 000, and 2000 Detectors 955-0023 ea. 25484 Description Model # Similar to TSP part # qty. cat.# price Check Valve Cartridge LDC Constametric Pumps 900946 ea. 25485 Sapphire Plunger LDC Constametric Pumps 80306 ea. 25486 Plunger Seal Kit, Gold LDC Constametric Pumps 3-36-00754 ea. 25487 Plunger Seal, Black LDC Constametric Pumps 2062900 ea. 25488 Plunger Seal, Gold LDC Constametric Pumps 2065600 ea. 25489 Lamp, Deuterium LDC SM-I, II, III, 3000, 300, 300X, and 4000 Detectors 08035 ea. 25490 Lamp, Deuterium Pre-aligned LDC 3200 and 400 Detectors 9009800 ea. 2549 Lamp, UV Piston Syringe Assembly, 250μL HPLC ACCESSORIES Restek Rheodyne Style Replacement Parts for Waters HPLC Systems Description Similar to Rheodyne part # qty. cat.# price 700 Vespel Rotor Seal 700-039 ea. 25279 725 Vespel Rotor Seal 725-047 ea. 25280 Isolation Seal, 700 700-05 ea. 2528 For easy access to Rheodyne fittings, use a ValvTool Wrench see page 54. Restek Replacement Parts for Waters Detectors Similar to Description Model # Waters part # qty. cat.# price Window Gasket 484, 486, 490 80335 ea. 25423 Lamp Side Gasket 484, 486, 490 80336 ea. 25424 Quartz Lens 486 80687 ea. 25427 Xenon Lamp (w/o holder or mirror) 470 ea. 25404 Xenon Lamp 474 ea. 25405 Deuterium Lamp (UV/Vis) 480, 48 99499 ea. 25403 Deuterium Lamp (UV/Vis) 484 80357 ea. 25406 Deuterium Lamp (UV/Vis) 486 80678 ea. 25407 Deuterium Lamp 996, 2996 WAT052586 ea. 25408 Deuterium Lamp 2487 WAS0842 ea. 25409 Deuterium Lamp, long life (2000 hours)* 486 80678 ea. 2540 *Standard lamps have nominal 000 hour life. also available HPLC autosampler syringes. See pages 64-65.

44 Replacement Parts HPLC ACCESSORIES Preventative Maintenance Kit for 77 Autosampler Inlet Check Valve Assembly new! 77 Seal Pack (Rebuilt) with Needle new! Restek Replacement Parts for Waters HPLC Systems Description Model # Similar to Waters part # qty. cat.# price Preventative Maintenance Kit Includes: sparge diffuser, filter insert, compression screws, SS ferrule, battery for 2690/77, 250µL WISP syringe, seal wash plunger seal kits (2), wash tube seal kits (4), 2690 seal pack rebuild kit (steel bodies not included), 2690 head plunger seal kits (2), solvent reservoir 20µm filters (4), Alliance check valve cartridges (2), Alliance plunger assemblies (2), 2690 face seals (4) Alliance 2690, 2695 WAT270944 kit 2543 Preventative Maintenance Kit Includes: PerformancePLUS cartridges (2), sparge diffusers (4), Super Seals (2), solvent reservoir 20µm filters (2), sapphire plungers (2), reference valve rebuild kit, inlet manifold kit 600 Pump WAT052675 kit 2544 Preventative Maintenance Kit Includes: 77 seal pack with needle, filter insert, 250µL WISP syringe 77 Autosampler WAT052669 kit 2545 Preventative Maintenance Kit Includes: 66/326/625 plunger assemblies (2), pump seal kit, sparge diffusers (4), solvent reservoir 20µm filters (4), 66 cartridge assemblies (4) 66 Pump WAT052672 kit 2546 Preventative Maintenance Kit Includes: PerformancePLUS check valves (2), sparge diffuser, solvent 0µm filter, sapphire plungers (2), plunger seals (2) 55 Pump WAT052587 kit 2659 Inlet Check Valve Assembly M6KA, 50, 50, 55, 590, 600E 33679, 2524 ea. 25360 Inlet Check Valve Housing M6KA, 50, 50, 55, 590, 600E 25203 ea. 2536 Inlet Check Valve Rebuild Kit M6KA, 50, 50, 55, 590, 600E 60495 2-pk. 25362 Outlet Check Valve Assembly (Actuator Style) M6KA, 50, 50, 55, 590, 600E 25030 ea. 25363 Outlet Check Valve Housing (Actuator Style) M6KA, 50, 50, 55, 590, 600E 2522 ea. 25364 Outlet Check Valve Rebuild Kit (Actuator Style) M6KA, 50, 50, 55, 590, 600E 2606 2-pk. 25365 Outlet Check Valve Assembly (Ball & Seat Style) M6KA, 50, 50, 55, 590, 600E 2526 ea. 25366 Outlet Check Valve Housing (Ball & Seat Style) M6KA, 50, 50, 55, 590, 600E 25207 ea. 25367 Outlet Check Valve Rebuild Kit (Ball & Seat Style) M6KA, 50, 50, 55, 590, 600E 2604 2-pk. 25368 Inlet Check Valve Assembly, 225µL (Extended Flow) M6KA, 50, 50, 55, 590, 600E 60307 ea. 25369 PerformancePLUS Check Valve Cartridge M6KA, 50, 50, 55, 590, 600E 700000254 2-pk. 25370 Check Valve Rebuild Kit (Extended Flow) M6KA, 50, 50, 55, 590, 600E 88223 2-pk. 2537 PerformancePLUS Check Valve Housing M6KA, 50, 50, 55, 590, 600E ea. 25372 Check Valve Cartridges Alliance WAT27094 2-pk. 25373 Super Seal for Analytical Heads M6KA, 50, 50, 55, 590, 600E 22946, 22934 ea. 25374 Plunger Seal, Gold for Analytical Heads* M6KA, 50, 50, 55, 590, 600E 22934 ea. 25375 Plunger Seal, Black for graphite-filled Teflon M6KA, 50, 50, 55, 590, 600E 2663 ea. 25378 Plunger Seal, Black for EF Heads 50, 590, 600E 26644 ea. 25379 Plunger Seal, Gold for EF Heads 50, 590, 600E 26644 ea. 25380 Seal Wash Plunger Seal Alliance WAT2708 2-pk. 25386 Head Plunger Seal Kit Alliance WAT270938 2-pk. 25387 Head Plunger Seal Kit (Black) Alliance WAT27066 2-pk. 25388 Insert Seal Parts Kit M6KA, 50, 50, 55, 590, 600E 6002 kit 25389 Sapphire Plunger M6KA, 50, 590, 600 25656 ea. 2538 Sapphire Plunger (Extended Flow) 50, 590, 600E 60304 ea. 25382 Sapphire Plunger M45, M50 26524 ea. 25383 Sapphire Plunger Assembly Kit M55 WAS207069 ea. 25384 Sapphire Plunger 66, 625, 626 3788 ea. 25420 Sapphire Plunger Alliance WAT270959 ea. 25385 Pressure Transducer M6KA, M45, 50, 50, 590, 600E WAT060329 ea. 2539 Draw-Off Tube Assembly 50, 55, 600, 60 25470 ea. 25392 Inlet Manifold Kit M45, 50, 50, 590, 600E 60448 kit 2542 Ferrule, Stainless Steel 55 22330 ea. 2547 Wash Face Seal Alliance 2690 WAT2707 ea. 25428 Wash Tube Seal Kit Alliance 2690 WAT270940 4-pk. 25429 Rebuild Kit, Reference Valve 600 Pump 25746 ea. 25492 Compression Screws, Stainless Steel Alliance 2690 2-pk. 25493 Battery Alliance 2690, 77, 486, 484 ea. 25494 Seal Pack Rebuild Kit, without Seal Wash Tube Assembly Alliance 2690 WAT2709 ea. 25495 77 Seal Pack (Rebuilt) with Needle 77 Autosampler 4559 ea. 25496 Seal Kit, Pump 66 Pump, 626, 625 3790 ea. 25497 Cartridge Assembly, Stainless Steel 66 Pump 24960 ea. 25498 *Ultra-high molecular weight polyethylene (UHMWPE).

Valves 45 ASI Piston Seals for Agilent HPLC Systems Over time the piston seal in every HPLC system becomes worn and must be replaced. Our piston seals are constructed of Teflon material with an additive that reduces their tendency to cold flow and improves their wear characteristics. Teflon seals are more resistant to any HPLC solvent than UHMWPE seals, but they are less wear resistant and rugged. For Agilent 050 and 00 HPLC systems. Description qty. cat.# price ASI Piston Seal ea. 2575 ASI Piston Seal 0-pk. 2576 ASI Check Valves ASI subjects each valve to a rigorous series of tests, including self-priming capability, which means you typically won t need a syringe or draw-off valves to prime your pump. A high-pressure test ensures that the valve will not leak, even at pressures up to 2,000psi (82,737kPa). A low-pressure test verifies that the valve closes properly, even without a high backpressure force. The cartridge design allows easy, convenient replacement of worn or damaged valves. ASI Check Valves for Agilent 050 & 00 Description Material qty. cat.# price Outlet Check Valve (cartridge and housing) Sapphire & Ruby Ball & Seat ea. 2570 Outlet Check Valve (cartridge and housing) Ceramic Ball & Seat ea. 2573 Outlet Cartridges Sapphire & Ruby Ball & Seat ea. 257 Outlet Cartridges Ceramic Ball & Seat ea. 2574 Outlet Housing ea. 2572 ASI Check Valves for Waters Alliance Model 2690 Description Material qty. cat.# price Inlet and Outlet Cartridges Sapphire & Ruby Ball & Seat ea. 2560 Inlet and Outlet Cartridges Ceramic Ball & Seat ea. 2562 Inlet and Outlet Check Valves for Waters Instruments HPLC ACCESSORIES Valves for HPLC Our valves have low internal volumes and are rated to 5,000psi (03,42kPa). They feature a 2-piece stem assembly in which the rotating upper shank is coupled through a ball joint to a static lower stem. Only fingertight torque is required to make the seal. new! Description qty. cat.# price Two-Way Through Valve for HPLC, /6" Fittings, /4-28, Stainless Steel, Includes Nuts and Ferrules ea. 26400 Two-Way Angle Valve for HPLC, /6" Fittings, /4-28, Stainless Steel, Includes Nuts and Ferrules ea. 2640 Three-Way Bottom Vent Valve for HPLC, /6" Fittings, /4-28, Stainless Steel, Includes Nuts and Ferrules ea. 26402 Side Vent Valve for HPLC, /6" Fittings, /4-28, Stainless Steel, Includes Nuts and Ferrules ea. 26403 Dual Stem Three Way Valve for HPLC, /6" Fittings, /4-28, Stainless Steel, Includes Nuts and Ferrules ea. 26404 Prime/Purge Valve for HPLC, /4-28 Flanged Seat, Stainless Steel, with Tubing and Fittings ea. 26406 Prime/Purge Valve Repair Kit for Prime/Purge Valve kit 26407 Side Vent Valve In-Line Check Valves for HPLC Our 36 stainless steel in-line check valves have a soft seat and /6" fittings. Nuts and ferrules are included. Description qty. cat.# price Check Valve, Soft Seat, for HPLC, /6" Fittings, /4-28, Stainless Steel, Includes Nuts and Ferrules ea. 26405 new!

46 Filters High-Pressure Frit-Type In-Line Filter Restek s high-pressure in-line filter is a stand-alone version of the Trident column protection system, specifically designed for ease of use, low dead-volume, and flexibility. The filter has a replaceable, PEEK encapsulated 36 stainless steel frit with a surface area of 2mm². The standard frit with 2.0µm porosity may be replaced with an optional 0.5µm porosity frit. Use of this filter can greatly extend column life, thereby reducing costs and saving maintenance time. Tubing OD /6"; connectors CPI. Description Porosity qty. cat.# price Frit-Type In-Line Filter 2.0µm ea. 2504 Replacement Cap Frits: 4mm 0.5µm 5-pk. 25023 Replacement Cap Frits: 4mm 2.0µm 5-pk. 25022 HPLC ACCESSORIES High-Pressure Cup-Type In-Line Filter This high-pressure cup-type filter can be used in fluid streams operating to 5,000psi (03,42kPa). The cup-shaped filter elements have a large (2.5 cm 2 ) surface area to give long operating lifetime. Mounted in screw-type adapters, they are easily removed for cleaning. Normally, backflushing and cleaning in an ultrasonic bath with an appropriate solvent will restore them. If they become permanently clogged, replacement elements are available. Housings and all wetted parts are 36 stainless steel. Filters are packaged with gland nuts and ferrules. A bulkhead type fitting is available for thru-panel mounting. Tubing OD /6"; connectors CPI. Description Porosity qty. cat.# price Cup-Type In-Line Filter 0.5µm ea. 25000 Cup-Type In-Line Filter 2.0µm ea. 2500 Replacement Filter Elements & Seals 0.5µm 2-pk. 25002 Replacement Filter Elements & Seals 2.0µm 2-pk. 25003 Low-Pressure Slip-On Inlet Filter for Mobile Phase Reservoir A type 36 stainless steel tip with a Tefzel collar seals to a corrosion-resistant type 36 stainless steel filter element. The slip-on filter easily attaches to the pump inlet line, without the use of wrenches. The universal tip accommodates standard Teflon tubing inner diameters. The cylindrical filter is standard 0µm porosity. /8" OD. Fits Altex, ISCO, LDC, Varian, Waters, PerkinElmer, and other pumps. Description qty. cat.# price Slip-on Inlet Filter ea. 25008 Low-Pressure CPI Inlet Filter for Mobile Phase Reservoir A 36 stainless steel knurled cap and Tefzel CPI ferrule seal to /8" OD Teflon tubing when finger-tightened onto the precision-machined filter holder. The filter element is replaceable. Standard 0µm porosity protects delicate pump components from particles but introduces very little pressure drop. /8"OD.May be used as a helium sparging diffuser. Description qty. cat.# price CPI Inlet Filter ea. 25009 Replacement Elements: 0µm filter 2-pk. 2500 also available Trident Direct high-pressure filter protection against particulate matter. See page 38 for details.

Filters 47 Mobile Phase Sparge Filter; Inlet Filter The 2µm helium sparge filter is an inexpensive way to prepare and maintain mobile phases free of dissolved gas. Both filters are made from 36 stainless steel and PEEK and are compatible with most solvents. Description qty. cat.# price Mobile Phase Sparge Filter: 2µm ea. 253 Inlet Filter: 0µm ea. 2532 Inlet Filter: 20µm ea. 2533 Last Drop Filter The flat filter element sits parallel to the bottom of the mobile phase reservoir, allowing the filter to draw 98% of the mobile phase without drawing air into the system. Conventional cylindrical mobile phase filters begin to draw air into the system when approximately 0% of the solvent remains in the reservoir. The Last Drop Filter allows more analyses per batch of mobile phase and helps reduce hazardous waste. 22.mm OD. Description qty. cat.# price Last Drop Filter: 2µm ea. 2534 Last Drop Filter: 0µm ea. 2535 HPLC ACCESSORIES Kontes Membrane Microfiltration Glassware 47mm filtration apparatus with fritted glass support is recommended for routine filtration of corrosive liquids and removal of particles from HPLC solvents. The ground joint connection eliminates phthalate contamination that can occur when using silicone or neoprene stoppers. The support base has a coarse porosity glass frit and an integral vacuum connection, located above the drip tip to prevent contamination of the vacuum line with filtrate droplets. Each apparatus includes a funnel, an anodized aluminum clamp, a 47mm fritted glass support base, and a filtration flask. Kontes All-Glass Microfiltration Apparatus qty. cat.# price 300mL Funnel, 000mL Flask ea. KT953825-0000 500mL Funnel, 2000mL Flask ea. KT953835-0000 000mL Funnel, 4000mL Flask ea. KT953845-0000 Replacement Parts for Kontes Microfiltration Apparatus qty. cat.# price 40/35 PTFE Joint Sleeve 6-pk. KT67600-4035 Flask Cap, 40/35 Outer Joint ea. KT953830-0000 Fritted Glass Support, 47mm, 40/35 Joint ea. KT953826-0000 Glass Funnel, 47mm, 00mL ea. KT95376-0000 Glass Funnel, 47mm, 300mL ea. KT95375-0000 Glass Funnel, 47mm, 500mL ea. KT95377-0000 Glass Funnel, 47mm, 000mL ea. KT95378-0000 Flask, 000mL, 40/35 Joint ea. KT953827-0000 Flask, 2000mL, 40/35 Joint ea. KT953828-0000 Flask, 4000mL, 40/35 Joint ea. KT953829-0000 Aluminum Clamp, 47mm ea. KT953753-0000 Membrane Filters qty. cat.# price Polyproylene Membrane Filters, 47mm, 0.45µm 00-pk. 26396 Polyproylene Membrane Filters, 47mm, 0.22µm 00-pk. 26397 Nylon Membrane Filters, 47mm, 0.45µm 00-pk. 26398 Nylon Membrane Filters, 47mm, 0.22µm 00-pk. 26399 new! tech tip Mobile Phase Additives Mobile phase additives such as triethylamine, trifluoroacetic acid, and ion-pairing reagents can compete with sample ions, decreasing sensitivity and, in some cases, reducing sample ion intake into the MS. To obtain symmetric peaks and/or sufficient retention, use base deactivated, state-of-the-art Type B silica packings that minimize the need for additives.

48 Mobile Phase Accessories HPLC ACCESSORIES Degasys Ultimate Degasser provides highly stable baselines Ultimate Degasser Off LC_096 Ultimate Degasser On Mobile Phase: water:methanol 50:50.0 ml/min. UV @ 20nm Mobile Phase Degasser Dissolved oxygen can cause flow rate instability and increased baseline noise. Also, it has a quenching effect on fluorescence detection and increases the background of UV detectors. Dissolved gases can out-gas in the HPLC system, forming bubbles in check valves, at connections, or in detector flow cells. In-line vacuum degassing is more effective at removing dissolved gas from mobile phases than sonication or helium sparging. In-line degassers work by withdrawing gas across a gas-permeable membrane encased in a sealed chamber. Traditionally, the membrane has been made of PTFE tubing, but the Degasys Ultimate Degasser uses tubing composed of an amorphous fluoropolymer that is 200 to 300 times more gas permeable than PTFE. This translates into the ability to use shorter tubing for removing dissolved gas. This new material also has better tubular burst strength than PTFE. To prevent cross contamination, each channel on this Degasys unit is individually encased within its own vacuum chamber. Specifications: Residual Oxygen Pressure Loss Internal Volume Wetted Parts Max Flow Rate 0.9ppm 0.24psi (.65kPa) 500µL Teflon AF, PTFE, ETFE, PPS 7mL/min./channel At a flow rate of ml/min. Description qty. cat.# price 0V Mobile Phase Degasser (4 Channel, 7mL/min./channel) ea. 2589 220V Mobile Phase Degasser (4 Channel, 7mL/min./channel) ea. 2594 Do not use the Degasys system with solutions containing TFA at concentrations greater than 5%. Solvent Debubbler Bubbles in an HPLC system can cause check valve malfunctions and pump cavitation, seriously affecting pump performance. The debubbler removes bubbles from the fluid stream before it enters the pump. Special geometry at the base of the housing allows bubbles entrained in the inlet fluid stream to rise and be trapped in the reservoir. The gas/liquid interface is easily visible through the translucent wall of the device. Loosening the airtight cap releases the trapped gas. The debubbler is fitted with a bracket and universal connecting tips. Description qty. cat.# price Solvent Debubbler with Bracket ea. 2504 Sonic Debubbler Fast. Easy to use. Less solvent waste; less clean-up. Just touch the Sonic Debubbler to the inlet line or check valve sonic vibrations will quickly dislodge or redissolve trapped air bubbles. Reduces downtime or conversion time from one mobile phase to another. Description qty. cat.# price Sonic Debubbler (0V) ea. 20444 Sonic Debubbler (220V) ea. 25098 Terry Reid Technical Service Representative 8+ years of service!

Bottle Tops 49 Opti-Cap Bottle Top The most economical way to helium-sparge and deliver HPLC mobile phases. The Opti-Cap top fits all standard GL45 bottles and has two /8-inch holes and one /6-inch hole for tubing. Description qty. cat.# price Opti-Cap (Cap and PEEK Plug) ea. 25300 Opti-Cap Kit (Opti-Cap, 3 meters of tubing, sparging filters) kit 2530 Opti-Cap Kit with L Bottle kit 25302 Opti-Cap Kit with 2L Bottle kit 25303 Related items and replacement parts qty. cat.# price SS Mobile Phase Mobile Phase Sparge Filter: 2µm ea. 253 Mobile Phase Inlet Filter: 0µm ea. 2532 Teflon Tubing, /8" OD x 0.094" ID x 3m (2.4mm ID) 3m 25307 Teflon Tubing, /8" OD x 0.063" ID x 3m (.6mm ID) 3m 25306 PEEK Plug, /4"-28 threads 3-pk. 2539 L Graduated Safety-Coated Bottle GL-45 threads ea. 25304 2L Graduated Safety-Coated Bottle GL-45 threads ea. 25305 Eco-Cap Bottle Top, PTFE Fits all standard GL-45 bottles; has two /8-inch holes and one /6-inch hole for tubing. Description qty. cat.# price Eco-Cap Bottle Top, includes Male Luer Plug ea. 25395 Opti-Cap Kit with bottle HPLC ACCESSORIES Hub-Cap 4 Liter Bottle Tops Most bottles use a GL45 cap. New Hub-Cap bottle tops are a great way to neatly keep your mobile phase lines where they belong. Use instead of parafilm, aluminum foil, or tape on your mobile phase reservoirs. new! Hub-Cap (assembly of the bottle cap and plug) Description qty. cat.# price Hub-Cap (assembly of the bottle cap and plug) kit 2654 Hub-Cap Multi-pack 3-pk. 26542 Hub-Cap Adapters Allow the use of the Opti-Cap with 4-liter solvent bottles. new! Description qty. cat.# price Hub-Cap Adapter ea. 26538 Hub-Cap Adapter Multi-pack 3-pk. 26539 Hub-Cap Adapter and Opti-Cap kit 26540 Hub-Cap Filter Kit Kit includes: bottle adapter, bottle adapter nut, filter inlet cap, grid support, vacuum hose barb, tube compression fitting, 47mm grid, 47mm.22µm filter membrane, 47mm.45µm filter membrane, /4" OD x /8" ID ultra chemical resistant, Teflon FEP lined Tygon tubing (3'), 6" x 6" box with shrink wrap insert. Description qty. cat.# price Hub-Cap Filter Kit kit 26395 Replacement Membrane Filters qty. cat.# price Polyproylene Membrane Filters, 47mm, 0.45µm 00-pk. 26396 Polyproylene Membrane Filters, 47mm, 0.22µm 00-pk. 26397 Nylon Membrane Filters, 47mm, 0.45µm 00-pk. 26398 Nylon Membrane Filters, 47mm, 0.22µm 00-pk. 26399 Hub-Cap Adapter new! Hub-Cap Adapter and Opti- Cap

50 Column Heater and Heater/Cooler, Gas Trapping System new! Sidewinder Column Heater Easy to set up! Operation range: 5 C above ambient to 85 C, ± C. Lightweight, compact design fits in small spaces. Column holder can be placed in any orientation. HPLC ACCESSORIES Sidewinder temperature control module new! This unique design completely encloses any HPLC analytical column up to 25cm in length. Two lengths of heater jackets are available: the short column holder accommodates columns up to 0cm in length, while the long column holder holds columns up to 25cm in length. The control module provides optimum heating performance, accuracy to within C, and stability to within 0. C. The new Sidewinder controller has fast 0Hz sampling for improved responsiveness. Power requirements: 24V control unit for maximum stability; RS232 control allows external programming. Description qty. cat.# price Temperature Control Module and Long Column Holder, 25cm Holder ea. 2656 Temperature Control Module and Short Column Holder, 0cm Holder ea. 2657 Sidewinder Heater/Cooler Temperature Control Module Operation range: 5-55 C, ±0.2 C. Ability to program multiple temperature points. Accommodates columns up to 30cm in length and 7.8mm ID. Compact design. The Sidewinder heater/cooler unit has a doubly insulated cover to maintain the programmed temperature to within 0.2 C. The 24V control unit provides maximum stability and rapid equilibration times; RS232 control allows external programming. Description qty. cat.# price Sidewinder Heater/Cooler Temperature Control Module ea. 2658 All Sidewinder temperature control products carry the value recognized CE mark. Each unit meets the demanding electromagnetic emission standards of the new European Union Directives, United States standards, and Canadian standards. Mobile Phase Pre-heater Heats mobile phase before it enters a heated column. Minimizes temperature changes, to help keep analyte peaks sharp. Description qty. cat.# price Mobile Phase Pre-heater ea. 22484 it s a fact A Super-Clean quick-change cartridge system efficiently removes hydrocarbons from nitrogen! Charcoal Filters Super-Clean Gas Trapping System for LC/MS Changing filters is quick and easy. Up to 20L of hydrocarbon-free nitrogen per minute. 2-Position Base Plate The Super-Clean Gas Trapping System is the latest technology in cartridge-style gas filtration for purifying nitrogen, and is ideal for use in LC/MS systems. The two-position baseplate (installed in the gas line) allows cartridges to be exchanged without introducing oxygen into the system. Spring-loaded check valves seal when a cartridge is removed and open only when a new cartridge has been locked in place. There is no need for loosening and tightening fittings every time you change cartridges, and your system cannot become contaminated during the changing process. To meet the high flow needs of the LC/MS system, the charcoal-filled cartridges are positioned and connected in parallel. The incoming gas stream is split equally between the cartridges, and the two streams are rejoined after purification but before the gas exits the baseplate. This approach allows longer contact between the nitrogen and the adsorbent, ensuring higher gas purity and eliminating a potential source of contaminants to your analyses. A handy date wheel, included with the system, indicates the cartridge installation date and the recommended replacement date. Description qty. cat.# price Super-Clean Gas-Trapping System (2-position baseplate, 2 charcoal filters) ea. 22062 2-Position Baseplate ( /4" Fittings) ea. 22060 Replacement Hydrocarbon (Charcoal) Filters 2-pk. 2206

Survival Kits 5 PEEK Survival Kit for HPLC The PEEK Survival Kit is an invaluable parts kit that contains tubing, fittings, and tools essential for setting up and maintaining your HPLC system: PEEK tubing, connectors, and elbows, Teflon tubing, a tubing cutter and extra blades, a ValvTool wrench, open-end wrenches, and more. For start-up and maintenance in all HPLC systems. Description qty. cat.# price PEEK Survival Kit for HPLC kit 25322 Contents: Description qty. cat.# price PEEK Column Connector (beige, round body) 0-pk. 2505 PEEK Tubing, /6" OD x 0.005" ID x 3m (red stripe) ea. 25065 PEEK Tubing, /6" OD x 0.007" ID x 3m (yellow stripe) ea. 25066 PEEK Tubing, /6" OD x 0.00" ID x 3m (blue stripe) ea. 25067 PEEK Tubing Elbow 90 5-pk. 25308 PEEK Tubing Elbow 80 5-pk. 25309 Teflon Tubing, /8" OD x 0.063" ID x 3m (.6mm ID) 3m 25306 Teflon Tubing, /8" OD x 0.094" ID x 3m (2.4mm ID) 3m 25307 Tubing Clip 5-pk. 2530 ValvTool Wrench ea. 2532 Open-End Wrenches ( /4" x 5 /6") 2-pk.* 200 Clean-Cut Tubing Cutter ea. 25069 Replacement Blade for Clean-Cut Cutter ea. 25070 PEEK Union Connector /6" 2-pk. 25323 Mobile Phase Sparge Filter: 2µm ea. 253 Inlet Filter: 0µm ea. 2532 *Kit contains wrench, replacement (cat.# 200) is 2-pk. PEEK Survival Kit HPLC ACCESSORIES Stainless Steel Survival Kit for HPLC The stainless steel survival kit contains a wide range of tubing, fittings, and tools necessary to set up and maintain your HPLC system: a selection of lengths and IDs of /6-inch tubing, plus nuts, ferrules, a ValvTool wrench, and a zero-dead-volume union. Description qty. cat.# price Stainless Steel Survival Kit for HPLC kit 25097 Contents: Description color qty. cat.# price HPLC Capillary Tubing, SS /6" x 0.005" x 5cm red 3-pk. 25240 HPLC Capillary Tubing, SS, /6" x 0.005" x 0cm red 3-pk. 2524 HPLC Capillary Tubing, SS, /6" x 0.005" x 20cm red 3-pk. 25242 HPLC Capillary Tubing, SS, /6" x 0.005" x 30cm red 3-pk. 25243 HPLC Capillary Tubing, SS, /6" x 0.007" x 5cm gray 3-pk. 25244 HPLC Capillary Tubing, SS, /6" x 0.007" x 0cm gray 3-pk. 25245 HPLC Capillary Tubing, SS, /6" x 0.007" x 20cm gray 3-pk. 25246 HPLC Capillary Tubing, SS, /6" x 0.007" x 30cm gray 3-pk. 25247 HPLC Capillary Tubing, SS, /6" x 0.00" x 5cm blue 3-pk. 25248 HPLC Capillary Tubing, SS, /6" x 0.00" x 0cm blue 3-pk. 25249 HPLC Capillary Tubing, SS, /6" x 0.00" x 20cm blue 3-pk. 25250 HPLC Capillary Tubing, SS, /6" x 0.00" x 30cm blue 3-pk. 2525 HPLC Capillary Tubing, SS, /6" x 0.020" x 5cm yellow 3-pk. 25252 HPLC Capillary Tubing, SS, /6" x 0.020" x 0cm yellow 3-pk. 25253 HPLC Capillary Tubing, SS, /6" x 0.020" x 20cm yellow 3-pk. 25254 HPLC Capillary Tubing, SS, /6" x 0.020" x 30cm yellow 3-pk. 25255 /6" Rheodyne Style Nut 0-pk. 25095 /6" Rheodyne Style Ferrule 0-pk. 25096 ValvTool Wrench ea. 2532 /6" Stainless Steel Ferrules 0-pk. 20286 /6" Stainless Steel Nuts 0-pk. 20287 Zero-Dead-Volume Internal Union ea. 2048 Stainless Steel Survival Kit it s a fact Survival Kits are convenient and economical, compared to ordering individual items!

52 Fittings, Connectors, Tools PEEK Fitting Extractor Drill into the broken fitting, then screw the extractor into the fitting and remove it easily. Eliminates the need for heat or other techniques that could damage your column. HPLC ACCESSORIES Description qty. cat.# price PEEK Fitting Extractor ea. 25325 PEEK Union Connector Allows you to quickly and reliably connect two pieces of /6-inch tubing. 0.3mm union bore. End fittings included. Description qty. cat.# price PEEK Union Connector /6" 2-pk. 25323 Zero-Dead-Volume Internal Union Ends of tubing seat squarely at bottoms of fitting details. 300 series stainless steel. For /6-inch OD tubing. Stainless steel ferrules included. Description Union Bore Valco # qty. cat.# price Internal Union 0.5mm ZUXC ea. 2047 Internal Union 0.25mm ZUC ea. 2048 Internal Union 0.75mm ZU ea. 2049 Internal Union /6" ZUT ea. 2050 Figure Incompatible fittings create problems. Tubing and ferrule seated correctly Improving Column Connections A good connection is necessary for trouble-free chromatography. Connecting incompatible fittings from different manufacturers can result in leaks, poor peak shape, and increased void volume (Figure ). Each type of end-fitting has a unique seat depth or style. Generally, Restek, Valco, Parker, and Upchurch Scientific fittings are interchangeable; whereas Waters, SSI, Rheodyne, and Gasukura fittings are not (Figure 2). Our wrenchless universal 0-32 PEEK column connector (cat.# 2505, page 54) can be used with any style of end fitting, and all /6-inch tubing. It is reusable and will adjust to any seat, depth, or style. Figure 2 Fitting styles differ among various manufacturers. Tubing not seated, causing dead volume Parker Valco Waters Swagelok Parker A-Lok Ferrule can t seal Rheodyne

HPLC Piston Seal Insertion Tool Simplify pump maintenance: use one end to remove your old seal, then simply slip your new seal on the other end and push it flush into position. The tool cannot mar the surrounding metal surface of the pump housing. Remove a seal Fittings, Connectors, Tools 53 Seat a seal Description qty. cat.# price HPLC Piston Seal Insertion Tool ea. 2356 Use the flat side of the Piston Seal Insertion Tool to seat a Waters face seal. HPLC ACCESSORIES Secure-Fit Fittings A good connection between HPLC components is necessary for trouble-free chromatography. Secure-Fit connectors from Restek and Selerity Technologies ensure a consistent, leak-free seal and they eliminate excess dead volume! An internal spring mechanism holds the capillary tubing at the proper depth in the female fitting. This seal is maintained while you finger-tighten the nut. These fittings are available in stainless steel or PEEK, and in a variety of tubing lengths and internal diameters. Stainless Steel Secure-Fit Fittings Single Connector Length 0.005" ID 0.007" ID 0.00" ID price 6cm 258 2585 2590 0cm 2582 2586 259 20cm 2583 2587 2592 30cm 2584 2588 2593 50cm 2650 265 2652 Stainless Steel Secure-Fit Fittings Two Connectors Length 0.005" ID 0.007" ID 0.00" ID price 0cm 25208 252 2524 20cm 25209 2522 2525 30cm 2520 2523 2526 50cm 2653 2654 2655 Stainless Steel Secure-Fit Fitting PEEK Secure-Fit Fittings Single Connector Length 0.005" ID 0.007" ID 0.00" ID price 6cm 25230 25234 2527 0cm 2523 25235 2528 20cm 25232 25236 2529 30cm 25233 25237 25220 PEEK Secure-Fit Fittings Two Connectors Length 0.005" ID 0.007" ID 0.00" ID price 0cm 2522 25224 25227 20cm 25222 25225 25228 30cm 25223 25226 25229

54 Connectors, Tubing Accessories PEEK Tubing Elbows Tubing Elbows (90 and 80 ) are ideal for routing /6-inch PEEK tubing through your system. Simply snap the tubing into the elbow. Prevent pinching of PEEK tubing which can cause high pressure. Description qty. cat.# price PEEK Tubing Elbow 90 5-pk. 25308 PEEK Tubing Elbow 80 5-pk. 25309 Tubing Dressing Tool Opens stainless steel tubing bore and removes burrs. For /6-inch OD tubing or /8-inch OD tubing. HPLC ACCESSORIES Description qty. cat.# price /6" Tubing Dressing Tool ea. 2088 Replacement Insert for /6" Tubing Dressing Tool ea. 2089 /8" Tubing Dressing Tool ea. 2090 Replacement Insert for /8" Tubing Dressing Tool ea. 209 Clean-Cut Tubing Cutter Burr-free, perpendicular cuts that will not distort the tubing OD or close the ID. Use on PEEK, Teflon, Tefzel, other polymeric tubing. Description qty. cat.# price Clean-Cut Tubing Cutter ea. 25069 Replacement Blade for Clean-Cut Cutter ea. 25070 For stainless steel tubing cutters, see our general catalog or website. Universal 0-32 PEEK Column Connectors and Plugs Universal PEEK Connectors allow easy installation of all /6-inch tubing, including stainless steel. See Improving Column Connections data on page 52. Description qty. cat.# price PEEK Column Connector (beige, round body) 0-pk. 2505 PEEK Column Plug (black) 0-pk. 2506 PEEK Fingertight Fittings (blue, flat-sided) 0-pk. 25324 Rheodyne Style Nut and Ferrule Replacement long nut for connecting stainless steel tubing to a Rheodyne 6-port valve or other Rheodyne part. Description qty. cat.# price /6" Rheodyne Style Nut 0-pk. 25095 /6" Rheodyne Style Ferrule 0-pk. 25096 ValvTool Wrench The ValvTool is a time-saving device that provides easy access to many hard-to-reach Rheodyne or Valco valves. For /4-inch nuts. Description qty. cat.# price ValvTool Wrench ea. 2532

Tubing 55 Inert PEEK Tubing Replaces stainless steel, titanium, Teflon or Tefzel tubing. Less oxygen permeable and more temperature resistant (to 250 C) than Teflon or Tefzel tubing. Use with PEEK fingertight or flangeless fittings. Use to 7,000psi (48,263kPa). Description Color Code qty. cat.# price PEEK Tubing, /6" OD x 0.0025" ID x m pink dash stripe 3-pk. 25320 PEEK Tubing, /6" OD x 0.005" ID x 3m red stripe ea. 25065 PEEK Tubing, /6" OD x 0.007" ID x 3m yellow stripe ea. 25066 PEEK Tubing, /6" OD x 0.00" ID x 3m blue stripe ea. 25067 PEEK Tubing, /6" OD x 0.020" ID x 3m orange stripe ea. 25068 HPLC Stainless Steel Capillary Tubing 36 grade stainless steel. Precise pre-cut lengths. Smooth surface finish. Ultra clean. Whether you need to replace system tubing as part of your troubleshooting or are looking to reduce the dwell volume of your system as you move to narrower columns, Restek has the quality tubing in the lengths and IDs you need. Each ID is color-coded so it is easy to identify and replace correctly. HPLC ACCESSORIES /6" OD 0.005" ID (red) 0.007" ID (gray) 0.00" ID (blue) 0.020" ID (yellow) Length qty. cat.# price cat.# price cat.# price cat.# price 5cm 3-pk. 25240 25244 25248 25252 0cm 3-pk. 2524 25245 25249 25253 20cm 3-pk. 25242 25246 25250 25254 30cm 3-pk. 25243 25247 2525 25255 Teflon Tubing Ideal for mobile phase inlet lines. Chemically inert. Use to 500psi (3,447kPa) and 80 C. Description qty. cat.# price Teflon Tubing, /8" OD x 0.063" ID x 3m (.6mm ID) 3m 25306 Teflon Tubing, /8" OD x 0.094" ID x 3m (2.4mm ID) 3m 25307 Tubing Clip Securely holds /6-inch or /8-inch tubing in beaker, flask, or bottle up to 4mm thick. Description qty. cat.# price Tubing Clip 5-pk. 2530

56 Flow Splitters, Static Mixers QuickSplit Post-Column Flow Splitters Fluid resistor technology eliminates adjustments to capillary tubing for optimizing split ratio. Wide range of interchangeable resistors available. Fixed Flow Splitters for HPLC & LC/MS Split ratio not affected by changes in viscosity or pressure. High operating pressure limit: 0,000psi (68,948kPa). Low dead volume - negligible effect on analyte bandwidth. Total flow: 0. 5.0mL/min. HPLC ACCESSORIES did you know? We are pleased to offer HPLC accessory items from Analytical Scientific Instruments (ASI). ASI specializes in HPLC system components and parts that often exceed the original equipment manufacturer s performance. See page 45. Adjustable Flow Splitter Adjustable metering valve gives convenient control of split ratio. Split ratio not affected by changes in viscosity or pressure. High operating pressure limit: 5,000psi (34,474kPa). Low dead volume - negligible effect on analyte bandwidth. Total flow: 0. 5.0mL/min. Description Split Ratio qty. cat.# price Binary Fixed Flow Splitter 00:0:00 ea. 25326 50:0:00 ea. 25327 20:0 ea. 25328 0:0 ea. 25329 5:0 ea. 25330 Replacement Fixed Flow Splitter Resistor Set 00:0:00 ea. 2533 50:0:00 ea. 25332 20:0 ea. 25333 0:0 ea. 25334 5:0 ea. 25335 Adjustable Flow Splitter 5: to 00: ea. 25336 new! : to 20: ea. 25337 50: to 000: ea. 2646 Replacement Adjustable Flow Splitter Resistor Set 5: to 00: ea. 25338 : to 20: ea. 25339 new! HyperShear HPLC Mixers Reduced baseline noise. Increased sensitivity. Improved gradient accuracy, for more reproducible results. Increased reaction efficiency in post-column derivatization. An efficient cross-flow shearing mechanism and interchangeable cartridges produce vortex shear mixing over a wide range of flows. Stainless steel or PEEK. HyperShear Static In-Line Mixers Stainless Steel PEEK Description qty. cat.# price qty. cat.# price µl In-Line Mixer ea. 26409 ea. 2640 25µL In-Line Mixer ea. 264 ea. 2642 50µL In-Line Mixer ea. 2534 ea. 2643 50µL In-Line Mixer ea. 25342 ea. 2644 250µL In-Line Mixer ea. 25343 ea. 2645 Ternary Tee Mixer Description qty. cat.# price 25µL Ternary Tee Mixer ea. 2538

FlatLine Pulse Damper Rupture-proof, no diaphragm minimal risk of failure or leaks. Clean flush-out design no sample carryover. Low internal volume negligible effect on analyte bandwidth. Pulse Dampers, Backpressure Regulators 57 The ASI FlatLine Pulse Damper combines performance and reliability in a simple, easy-to-use housing. Standard 0-32 inlet and outlet ports allow quick connection into virtually any HPLC system. Solid core technology provides reliable long-term operation without the down time associated with ruptured or leaking membrane dampers. 600 700µL internal volume at ambient pressure. Description qty. cat.# price FlatLine Pulse Damper ea. 25340 MicroPulse Pulse Dampers Compact unit (2.5" x.5") can be placed almost anywhere. Small, 50µL dead volume at atmospheric pressure. PEEK unit can withstand pressures to 5,000psi (34,474kPa). 36 Stainless steel unit can withstand pressures to 6,000psi (4,369kPa). Improves system baseline stability while increasing the total system volume by only 50µL. The MicroPulse pulse damper is ideal for applications where minimizing the total system volume is critical. Stainless steel and PEEK options for a wide range of applications. HPLC ACCESSORIES Description qty. cat.# price MicroPulse Pulse Damper, Stainless Steel ea. 25238 MicroPulse Pulse Damper, PEEK ea. 25239 LO-Pulse Pulse Damper The LO-Pulse Pulse Damper is a patented, wide-dynamic-range 36 stainless steel device that smooths pulsations and maintains constant flow at system pressures up to 6,000psi (4,369kPa). The flow path volume is only 0.9mL and the path is efficiently swept, eliminating solvent memory effects when changing mobile phases. The LO-Pulse pulse damper also is available in a space-saving, economical kit that includes hardware for mounting the pulse damper on a bracket, or for installing feet on it for bench-top use. Description qty. cat.# price Model LP-2 LO-Pulse Pulse Damper ea. 2502 Pulse Damper Kit kit 2503 LO-Pulse Pulse Damper Kit Backpressure Regulators Backpressure regulators can improve detector performance by preventing bubble formation in the detector flow cell. They also are useful in post-column reaction lines and between detectors and fraction collectors in preparatory work. Regulators are superior to more specific alternative solutions, like smallbore tubing, in which pressure varies with flow rate. Our end-of-line and flow-through backpressure regulators are adjustable to assure constant backpressure over a wide range of mobile-phase viscosities and flow rates. The end-of-line model is available with /4-28 plastic flange-type fittings or high-pressure /6-inch compression fittings; this design adjusts from 5 to 60psi (03 to 44kPa). The flow-through design has /6-inch compression fittings and is adjustable from 7 to 75psi (48 to 57kPa). Description qty. cat.# price A) Backpressure Regulator: end-of-line, /6-inch OD tubing, flanged ea. 2507 B) Backpressure Regulator: end-of-line, high-pressure seat ea. 2508 C) Backpressure Regulator: flow-through, 5µL internal volume ea. 25020 AA B B C C

58 Column Hardware HPLC Column Tubing Restek tubing is manufactured from fine, chromatographic-grade 36 stainless steel. It is corrosion resistant, ultrasonically cleaned and passivated. We polish the tubing and promise a burr-free cut. You can use this tubing immediately there is no need for any additional treatment. HPLC ACCESSORIES 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price /4" OD Tubing 30mm 2500 2506 252 50mm 250 2507 253 00mm 2502 2508 254 50mm 2503 2509 255 200mm 2504 250 256 250mm 2505 25 257 /4-Inch HPLC Frits We manufacture our frits from fine, chromatographic-grade 36 stainless steel, and we offer sizes to fit most column and pore sizes. To choose the correct frit, check its pore size compatibility with the particle size of the packing in the column (see chart at left). If the packing has a smaller particle size than the pore size of the frit, the packing can clog the frit. Packing particle size 3 4µm 5 20µm Use this pore size: 0.5µm 2.0µm ID Pore Size qty. cat.# price 4.6mm 2.0µm 0-pk. 2507 4.6mm 0.5µm 0-pk. 25072 3.2mm 2.0µm 0-pk. 25073 3.2mm 0.5µm 0-pk. 25074 2.mm 2.0µm 0-pk. 25075 2.mm 0.5µm 0-pk. 25076 Column End-Fittings /4-inch end-fittings are compatible with Valco and Parker fittings. Description qty. cat.# price Column End-Fitting for 4.6mm Column ea. 25077 Column End-Fitting Flat Bottom ea. 25078 Empty Chromatography Columns 36 stainless steel tubing complete with end-fittings, frits, nuts, and ferrules. Preassembled prior to shipment, unless otherwise requested. Internal /6-inch seats are compatible with Valco and Parker fittings. 2.mm ID 3.2mm ID 4.6mm ID Length cat.# price cat.# price cat.# price /4" OD Tubing 30mm 258 2524 2530 50mm 259 2525 253 00mm 2520 2526 2532 50mm 252 2527 2533 200mm 2522 2528 2534 250mm 2523 2529 2535

Restek Pack in a Box Kit, HPLC Organizers 59 Restek Pack in a Box Kit Restek s new Pack in a Box Kit is a complete column-packing system. Everything you need to pack your own analytical HPLC columns is included: pump, reservoir, column hardware, packing material, and system control software (computer not included). Detailed packing instructions and a video also are included. The packing pump is electric, so no pressurized air is required. new! Description qty. cat.# price Pack in a Box Kit kit 26408 HPLC Organizers HPLC ACCESSORIES LCLocker HPLC Organizer Deluxe BenchBooster Organizer Mini phperch Storage Unit TopLoader BalanceBank Storage Unit Bill Grove Instrument Innovations + year of service! HPLC 30-Column Storage Cabinet Book Holders Open Supply Bins, 3-bin unit Glove Box Dispensers Description dimensions qty. cat.# price LCLocker 24 x 2 x 6" ea. 2549 BenchBooster 24 x 7 x 2" ea. 2550 Mini phperch 3 x 2 x 6" ea. 2547 TopLoader 2 x 2 x 7" ea. 2548 30 Column Cabinet 7 3 /8 x 5 x 2 7 /8" ea. 2559 Book Holder, Small 0.75" ID ea. 255 Book Holder, Large.5" ID ea. 2552 Open Supply Bin, 4-bin Unit 24 x 2 x 0" ea. 2553 Open Supply Bin, 5-bin Unit 2 x 6 x 0.5" ea. 2554 Open Supply Bin, 3-bin Unit 2 x 7.5 x 2" ea. 2555 Glove Box Dispenser, Single 5 3 /8 x /2 x 4 /2" ea. 2556 Glove Box Dispenser, Double 0 3 /8 x /2 x 4 /2" ea. 2557 Glove Box Dispenser, Triple 5 3 /8 x /2 x 4 /2" ea. 2558 *Please note: Accessories and supplies in photographs are not included.

60 Tech Tips HPLC Pump Pressure Conversion Table Pressure psi atm kg/cm 2 torr kpa bar inches Hg psi = 0.068 0.0703 5.73 6.8948 0.06895 2.0359 atm = 4.696.0332 760 0.32.033 29.92 kg/cm 2 = 4.223 0.967 735.5 98.06 0.9806 28.958 torr = 0.093 0.0032 0.0036 0.330 0.0033 0.0394 kpa = 0.450 0.00987 0.002 7.52 0.000 0.2962 bar = 4.5038 0.9869.097 75.88 00 29.5300 in Hg = 0.4962 0.0334 0.0345 25.400 3.376 0.03376 HPLC ACCESSORIES Acetone Acetonitrile Dimethyl formamide Dimethyl sulfoxide Dioxane Ethanol Isopropyl alcohol Methanol Tetrahydrofuran Water Benzene Butanol Carbon tetrachloride Chloroform Cyclohexane,2-Dichloroethane Dichloromethane Ethyl acetate Ethyl ether Heptane Hexane Isooctane Methyl tert-butyl ether Methyl ethyl ketone Pentane Toluene Xylene Multiply units in the left-most column by the conversion factors listed in the columns to the right. e.g., 0 psi x 0.068 = 0.68atm 0 bar x 29.5300 = 295.300 inches Hg Acetone Acetonitrile Dimethyl formamide Dimethyl sulfoxide Dioxane Solvent Miscibility and Solubility Ethanol Isopropyl alcohol Methanol Tetrahydrofuran Water Benzene Butanol Carbon tetrachloride Chloroform Cyclohexane,2-Dichloroethane Dichloromethane Ethyl acetate Ethyl ether Miscible/Water Miscible Solvents Miscible Solvents Immiscible Solvents Heptane Hexane Isooctane Methyl tert-butyl ether Methyl ethyl ketone Pentane Toluene Xylene

mm Vials, 9mm Vials 6 Crimp-Top Vials, 2.0mL, 2 x 32mm, mm Crimp Finish White graduated marking spots are a standard feature unless otherwise noted. Crimp-Top Vials, Snap Seal Style 2 x 32mm, mm Crimp Description 00-pk. 000-pk. 2.0mL Clear Glass Vial w/white Graduated Marking Spot 24383 24384 2.0mL Amber Glass Vial w/white Graduated Marking Spot 24385 24386 2.0mL Clear Glass Vial without Graduated Marking Spot 252 253 mm Aluminum Crimp Seals with Septa Description 00-pk. 500-pk. 000-pk. Silver Seal, PTFE/Natural Rubber Septa 274 275 Blue Seal, PTFE/Red Rubber Septa 2435 24352 Green Seal, PTFE/Red Rubber Septa 24353 24354 Red Seal, PTFE/Red Rubber Septa 24355 24356 Yellow Seal, PTFE/Red Rubber Septa 24357 24358 Mixed Colors, PTFE/Rubber Septa 2724 Silver Seal, PTFE/Silicone Septa 24359 24360 Blue Seal, PTFE/Silicone Septa 2436 24362 Green Seal, PTFE/Silicone Septa 24363 24364 Red Seal, PTFE/Silicone Septa 24365 24366 Yellow Seal, PTFE/Silicone Septa 24367 24368 Mixed Colors, PTFE/Silicone Septa 2725 Silver Seal, PTFE/Silicone/PTFE Septa 24369 24370 for more info see page 62 for electronic crimpers and decappers. HPLC ACCESSORIES Convenience Kits: Vials, Caps, & Septa Vials packaged in a clear-lid tray. Caps with septa packaged in a plastic bag. Description 00-pk. 000-pk. 2.0mL Clear Vial, deactivated, PTFE/Natural Rubber Silver Seal 2467 24672 2.0mL Amber Vial, deactivated, PTFE/Natural Rubber Silver Seal 24673 24674 2.0mL Clear Vial, untreated, PTFE/Natural Rubber Silver Seal 296 297 2.0mL Amber Vial, untreated, PTFE/Natural Rubber Silver Seal 298 299 2.0mL Clear Vial, untreated, PTFE/Silicone Silver Seal 24646 24647 2.0mL Amber Vial, untreated, PTFE/Silicone Silver Seal 24648 24649 9mm Short-Cap, Screw-Thread Vials, 2 x 32mm, 9mm Opening Fit all 2.0mL, 2 x 32mm, Crimp-Top Vial Based Autosamplers. Shorter screw cap allows the robotic autosampler to function properly. Wider opening prevents syringe problems. Dimensions are effectively identical to the crimp-top vial. Variety of colors and liner material. 9mm Short-Cap, Screw-Thread Vials, 2 x 32mm, 9mm Opening Description 00-pk. 000-pk. 2.0mL Clear Vial, w/white Graduated Marking Spot 240 24 2.0mL Amber Vial, w/white Graduated Marking Spot 242 243 2.0mL Clear Vial, without Graduated Marking Spot 254 255 9mm Thread Pre-Assembled Short-Cap, Screw-Vial Closures Description 00-pk. 500-pk. 000-pk. Blue Cap, PTFE/Red Rubber Liner 24473 24474 Mixed Colors, PTFE/Red Rubber Liner 24668 Blue Cap, PTFE/Silicone Liner 24485 24486 Green Cap, PTFE/Silicone Liner 24487 24488 Yellow Cap, PTFE/Silicone Liner 24493 24494 Mixed Colors, PTFE/Silicone Liner 24669 Blue Cap, PTFE/Silicone/PTFE Liner 24497 24498 Black Cap, PTFE/Silicone/PTFE Liner 24495 24496 Mixed Colors, PTFE/Silicone/PTFE Liner Cap 24670

62 Crimpers and Decappers Safer than removing caps with pliers, knife or screwdriver blade! Aluminum Seal Crimpers and Decappers The crimper is adjustable for optimized sealing performance and is comfortable enough for prolonged use. If you need to save, transfer, or dispose of your samples, our decapper allows you to remove a crimptop cap safely and easily. Far safer than removing caps with pliers, knife or screwdriver blades, etc. If you aren t currently using a seal decapper, we recommend you order one today! HPLC ACCESSORIES Decapper Crimper Description qty. cat.# price 8mm Crimper ea. 2735 8mm Decapper ea. 2736 mm Crimper ea. 270 mm Decapper ea. 27 3mm Crimper ea. 2739 3mm Decapper ea. 2740 20mm Crimper ea. 2737 20mm Decapper ea. 2738 Hand-held Rechargeable Crimpers and Rechargeable Decappers Easy to use; comfortable grip. Hundreds of operations from one charge. Adjustable crimping force. All kits include battery charger. Powered by a standard Black and Decker Versapak battery, these electronic tools for mm or 20mm caps will cycle hundreds of times on a single charge. The cycle is controlled precisely, through an internal counter, and is adjusted with two buttons on the side of the case. The tools fit comfortably in the hand and weigh approximately 600 grams. The jaws can be positioned easily around closely-spaced vials in standard autosampler trays. Each kit includes the tool, a Versapak Gold rechargeable battery, which uses environmentally friendly NiMH technology, and a charger. Recharging generally takes 6-9 hours. Decapping has never been easier! one... two... three! The Electronic Crimper fits around vials in standard autosampler trays. The adjustable setting provides a precision crimp, vial after vial. Description qty. cat.# price mm Electronic Crimper (0 volt battery charger) kit 22853 mm Electronic Crimper (220 volt European battery charger) kit 22853-EUR mm Electronic Crimper (220 volt UK battery charger) kit 22853-UK mm Electronic Decapper (0 volt battery charger) kit 22854 mm Electronic Decapper (220 volt European battery charger) kit 22854-EUR mm Electronic Decapper (220 volt UK battery charger) kit 22854-UK 20mm Electronic Crimper (0 volt battery charger) kit 2285 20mm Electronic Crimper (220 volt European battery charger) kit 2285-EUR 20mm Electronic Crimper (220 volt UK battery charger) kit 2285-UK 20mm Electronic Decapper (0 volt battery charger) kit 22852 20mm Electronic Decapper (220 volt European battery charger) kit 22852-EUR 20mm Electronic Decapper (220 volt UK battery charger) kit 22852-UK

WISP Style Vials 63 Screw-Thread Step Vials, 4.0mL, WISP 48 Style, 3mm/425 Screw-Thread WISP 48 Screw-Thread Step Vials (5 x 45mm, 3/425 Screw-Thread) Description 00-pk. 000-pk. 4.0mL Clear Step Vial, White Grad. Marking Spot 24654 24655 4.0mL Amber Step Vial, White Grad. Marking Spot 24656 24657 Caps and Septa Description 00-pk. 000-pk. Top Hat, PTFE/Silicone Septa 2745 2746 Pre-Assembled, Red PTFE/Silicone, 0.065" 2743 2744 Caps, Black, Polypropylene, Open-Hole 274 2742 Septa, Red PTFE/Silicone, 0.040" 275 2752 Septa, Red PTFE/Silicone, 0.060" 2749 2750 Limited Volume Inserts Description 00-pk. 000-pk. 500µL, Glass, WISP, Flat Bottom* 2787 2788 *Also fit 4.0mL Snap Seal Vials (see our general catalog or website). 4.0mL Clear Step Vial HPLC ACCESSORIES Shell Vials,.0mL, WISP 96 Style, 8 x 40mm WISP 96 Style, 8 x 40mm Shell Vials Description 200-pk. 000-pk..0mL Clear Vial 24662 24663.0mL Amber Vial 24664 24665.0mL Polypropylene Vial 24678 24679 700µL Limited Volume Polypropylene Vial 24680 2468 8mm Starburst Plugs Description 200-pk. 000-pk. Snap Plug, Clear Polyethylene 2769 2770 Snap Plug, Conical, Clear Polyethylene 277 2772 Snap Plug, Silicone Lined, Clear Polyethylene 2773 2774 Limited Volume Inserts for Shell Vials Description 00-pk. 000-pk. 250µL Glass, Big Mouth Insert w/ Bottom Spring 2776 2777

64 LC Autosampler Syringes Syringes for Agilent 090 & 00 LC Autosamplers SGE Syringes 25μL, /4-32 UNEF Thread SGE Restek Volume Model cat.# qty. cat.# price 25µL 25D-HP090-GT 003670 ea. 22290 250µL 250D-HP090-GT 006670 ea. 2229 HPLC ACCESSORIES Syringe for Hitachi LC Autosamplers SGE Syringe 500μL, M0 X Thread SGE Restek Volume Model cat.# qty. cat.# price 500µL 500C-HITACH 007660 ea. 22292 Syringes for PerkinElmer LC Autosamplers SGE Syringes 250μL, /4-28 UNF Thread SGE Restek Volume Model cat.# qty. cat.# price 50µL 50D-CX-GT 004995 ea. 22295 00µL 00D-CX-GT 005990 ea. 22296 250µL 250D-CX-GT 006995 ea. 22297 500µL 500D-CX-GT 007995 ea. 22298 ml MD-C-GT 00885 ea. 22299 Syringes for Waters WISP LC Autosamplers SGE Syringes 250μL, /4-28 UNF Thread SGE Restek Volume Model cat.# qty. cat.# price 25µL 25D-WISP 003990 ea. 22293 250µL 250D-WISP 006690 ea. 22294 Syringes for Waters WISP LC Autosamplers Teflon PTFE-tipped plungers Hamilton Syringes Hamilton Restek Volume Model cat.# qty. cat.# price 25µL 702 80020 ea. 24528 250µL 725 80024 ea. 24529

LC Autosampler Syringes / Analytical Reference Materials 65 Syringes for CTC/LEAP LC Autosamplers Hamilton Syringes Hamilton 0μL LC Autosampler Syringe Volume Needle Needle Needle Point Hamilton Restek Term. Gauge Length Style Model cat.# qty. cat.# price 0µL N 22s 2"/5mm 3 70N 203073 ea. 22743 25µL N 22s 2"/5mm 3 702N 203075 ea. 22744 00µL N 22s 2"/5mm 3 70N Slim Line* 203077 ea. 22745 00µL N 22 2"/5mm 3 70N 203235 ea. 22746 250µL N 22 2"/5mm 3 725N 203079 ea. 22747 500µL N 22 2"/5mm 3 750N 203349 ea. 22748 *Barrel O.D. = 6.7mm; all other 25µL and 00µL syringes have a 7.9mm barrel O.D. SGE Syringes SGE 0μL LC Autosampler Syringe Volume Needle Needle Needle Point SGE Restek Term. Gauge Length Style Model cat.# qty. cat.# price 0µL F 22s 2"/5mm LC 0F-CTC-LC 00270 ea. 22737 25µL F 22s 2"/5mm LC 25F-C/T-LC 003984 ea. 22738 25µL* R 22s 2"/5mm LC 25R-C/T-GT-LC 003985 ea. 22739 50µL* F 22s 2"/5mm LC 50F-C/T-GT-LC 00480 ea. 22740 00µL* R 22s 2"/5mm LC 00R-C/T-GT-LC 005330 ea. 2274 500µL* F 22s 2"/5mm LC 500F-CTC-GT-LC(0.4) 007720 ea. 22742 *Gas-Tight syringe. new! new! HPLC ACCESSORIES Analytical Reference Materials HPLC Normal Phase Test Mix # Routine analysis using this mix can assist in determining the need to perform column and/or system maintenance. benzene.00mg/ml benzyl alcohol 3.00 benzaldehyde 0.04 4-methoxybenzyl alcohol 2.00 In hexane, ml/ampul cat. # 35004 (ea.) No data pack available. HPLC Reversed Phase Test Mix # Routine analysis using this mix can assist in determining the need to perform column and/or system maintenance. benzene 3.00mg/mL naphthalene 0.50 uracil 0.02 biphenyl 0.06 In methanol:water (75:25), ml/ampul cat. # 35005 (ea.) No data pack available. HPLC Performance Test Mix The National Institute of Standards and Technology (NIST) formulated a mixture that is highly effective for characterizing HPLC columns for efficiency, void volume, methylene selectivity, retentiveness, and activity toward chelators and organic bases. Results can be used for column classification, column selection, monitoring column performance over time, or for quality control. We test our material against the NIST 870 standard. amitriptyline quinizarin 94 hydrochloride 2800µg/mL toluene 400 ethylbenzene 700 uracil 28 In methanol, ml/ampul cat. # 3699 (ea.) HPLC OQ Linearity Test Mix Kit Linear detector responses to concentration variations are an important part of operation qualification (OQ) for HPLC instruments. Our kit of five aqueous solutions of caffeine can be used to generate simple plots of UV response versus concentration. Certificate of Analysis includes caffeine concentration, calculated variance in preparing each mixture, a linearity plot, and coefficient of determination (r²) for the linear plot. Caffeine at 5.0, 25.0, 25.0, 250.0, 500.0 µg/ml in water in a five ampul kit. ml each of these mixtures. cat. # 3805 (kit) No data pack available. Quantity discounts not available. Carbohydrate HPLC Performance Check Mix Performance qualification (PQ) determines the precision of the HPLC system. Our performance check mix for HPLC / RI consists of five simple sugars in varied concentrations. We prepare the reference material in water, lyophilize it, and pack it dry for enhanced stability. glucose 2.0mg maltose 4.5 fructose 2. sucrose 4.0 lactose 4.4 Dry components in 4mL screw-cap vial. Reconstitute in ml acetonitrile:water (75:25) to 2.0, 2., 4.4, 4.5, 4.0 mg/ml, respectively. cat. # 3809 (ea.) No data pack available.

66 Nucleotides, Nucleosides, Nucleic Acid Bases Nucleotides, Nucleosides, and Nitrogenous Bases on Ultra IBD Uracil, Uridine, UTP, UDP, and UMP on Ultra IBD HPLC APPLICATIONS BIOCHEMICAL/CLINICAL Conc. (µg/ml). CDP 46 2. CMP 3 3. ATP 7 4. ADP 5 45 5. cytidine 55 6. TMP 57 7. AMP 25 8. guanine 36 9. guanosine 03 0. adenine 38. adenosine 5 2 3 4 6 7 8 9 Column: Ultra IBD Cat. #: 975565 Dimensions: 50 x 4.6mm Pore size: 00Å 0 Inj.: 20µL Solvent: 20mM ammonium acetate, ph 5.8 0 2 4 6 8 0 2 4 6 8 min. LC_028 Thymine, Thymidine, TTP, TDP, and TMP on Ultra IBD 2 3 4 5 20mM ammonium acetate, ph 5.8:methanol (97.5:2.5).0mL/min. Temp.: 35 C UV @ 260nm Conc. (µg/ml). TTP 224 2. TDP 52 3. TMP 346 4. thymine 88 5. thymidine 38 Inj.: 20µL Solvent: 20mM ammonium acetate, ph 5.8 Column: Ultra IBD Cat. #: 975565 Dimensions: 50 x 4.6mm Pore size: 00Å 20mM ammonium acetate, ph 5.8: methanol (97.5:2.5).0mL/min. Temp.: 35 C UV @ 260nm 2 3 4 5 0 2 4 6 min. LC_033 Inj.: Conc. Solvent: Conc. (µg/ml). UTP 360 2. UDP 360 3. UMP 284 4. uracil 90 5. uridine 230 Inj.: 20µL Solvent: 20mM ammonium acetate, ph 5.8 Column: Ultra IBD Cat. #: 975565 Dimensions: 50 x 4.6mm Pore size: 00Å 20mM ammonium acetate, ph 5.8: methanol (97.5:2.5).0mL/min. Temp.: 35 C UV @ 260nm Nucleic Acid Bases on Allure PFP Propyl. cytosine 2. uracil 3. guanine 4. thymine 5. adenine 2 3.0µL 00µg/mL each 20 mm ammonium acetate, ph 5.0 Note: -3 drops of ammonium hydroxide added to keep guanine in solution Column: Allure PFP Propyl Cat. #: 969565 Dimensions: 50 x 4.6mm Pore size: 60Å 4 Temp.: 20mM ammonium acetate, ph 5.0: methanol (85:5, v/v).0ml/min. ambient UV @ 260nm 5 0 2 4 6 8 0 2 4 6 min. LC_032 0 2 3 4 5 6 7 8 9 min. LC_037

Purines/Pyrimidines, Amino Acids, Cocaine 67 Purines/Pyrimidines on Ultra PFP 2 3 4 5 6 7 8 9 0 0 5 0 5 20 25 min. Conc. (mg/ml). tyrosine 0.2 2. phenylalanine.6 3. tryptophan 0.4 Inj.: 20µL Solvent: mobile phase A LC_036 Aromatic Amino Acids on Ultra Aqueous C8 Column: Ultra Aqueous C8 Cat. #: 978565 Dimensions: 50 x 4.6mm Pore size: 00Å Mobile phases: A: 50mM potassium phosphate, ph 2.5 B: acetonitrile 5% - 20% B: 0-5 min. 20% - 5% B: 5-6 min. Hold at 5% B: 6-3 min..0ml/min. Temp.: 30 C UV @ 254nm Searching for a chromatogram? 2 3 2 3 4. cytosine 2. 5-methylcytosine 3. uracil 4. uric acid 5. hypoxanthine 6. xanthine 7. thymine 8. purine 9. theobromine 0. ethyluracil. 6-methylpurine 2.,7-dimethylxanthine 3. theophylline 4. propyluracil Inj.: 0µL Conc.: 00µg/mL each Solvent: mobile phase Column: Ultra PFP Cat. #: 976575 Dimensions: 250 x 4.6mm Pore size: 00Å Temp.: 0.00% formic acid in water: methanol (50:50, v/v).0ml/min. ambient UV @ 260nm Cocaine and Ecgonine Methyl Ester on Allure PFP Propyl. EME (ecgonine methyl ester) 2. COC (cocaine) Inj.: 0µL Conc.: µg/ml Solvent: water Temp.: 4 C Column: Allure PFP Propyl Cat. #: 969532 Dimensions: 30 x 2.mm Pore size: 60Å LC_026 Column temp.: XIC of +MRM (3 pairs) for 200.0 / 82.0 amu.30e3 cps 5mM ammonium formate, ph 3.0: acetonitrile (0:90, v/v) 0.6mL/min. ambient Applied Biosystems API 3200 LC/MS/MS System turbo ion spray, ESI Interface: Interface temp.: 50 C Ion mode: positive ESI probe voltage: 5000V Orifice: + 7V Ring: + 265V Collision gas: nitrogen Collision gas pressure: 2.2 mtorr Collision gas energy: 28 ev (COC) 26 ev (EME) Electron multiplier: 200 volts Auxilary gas flow: 7000cc/min. Nebulizer gas setting: 5lb/in.² Curtain gas setting: 2lb/in.² HPLC APPLICATIONS BIOCHEMICAL/CLINICAL 0 0.5.0.5 2.0 2.5 3.0 3.5 4.0 4.5 min. XIC of +MRM (3 pairs) for 304.0 / 82.0 amu 2.35e3 cps 2 0 2 4 6 8 0 min. LC_046 0 0.5.0.5 2.0 2.5 3.0 3.5 4.0 4.5 min. Data Courtesy of Shane Needham, Pfizer Inc.

68 Proteins, Peptides, Catecholamines Protein Mix on Viva C8 Viva Wide Pore C8 (cat.# 954562) Note excellent peak shapes and resolution on the Viva column! HPLC APPLICATIONS BIOCHEMICAL/CLINICAL mfr. H mfr. D mfr. C mfr. F Viva Manufacturer A Manufacturer B Manufacturer C Manufacturer D Peptide Mix on Viva C8 LC_0324 2 3 4 5 6 0.0 2.0 4.0 6.0 8.0 0.0 2.0 4.0 6.0 8.0 20.0 LC_BC0386 Inj.: 20µL peptide test mix Column: Viva C8 Wide Pore Cat.#: 954562 Dimensions: 50 x 2.mm Pore size: 300Å. val-tyr 2. val-tyr-val 3. meth enkephalin 4. val-4-angiotensin III 5. angiotensin II, III 6. leucine enkephalin 7. angiotensin I A - 0.08% trifluoroacetic acid in water (ph 2.0) B - 0.08% TFA in acetonitrile, 0% B to 40% B in 20 min. 0.2mL/min. UV @ 24nm * High levels of trace metals (~00ppm) in the silica enhance this separation. * 7 Peak List Ret. Time (min.). ribonuclease A.3 2. cytochrome c 4.65 3. holo-transferrin 6.32 4. apomyoglobin 20.34 Inj.: 20µL Conc.: 0.08mg/mL each protein Sample diluent: 0.0% TFA in water / 0.0% TFA in acetonitrile, 80:20, v/v Sample temp.: 25 C Columns: Wide Pore C8 Dimensions: 50 x 2.mm Pore size: 300Å Temp.: Catecholamines on Allure PFP Propyl LC_BA0349 A: 0.0% TFA in water, B: 0.0% TFA in acetonitrile, 20% B to 70% B in 30 min. 0.20mL/min. 25ºC (or ambient) UV @ 24nm Peak List Retention Time (min.). norepinephrine 3.50 2. levodopa 5.43 3. epinephrine 7.27 4. tyrosine 7.77 5. dopamine.3 Inj.: 0µL Conc.: 40µg/mL each component Sample diluent: mobile phase Sample temp.: ambient Column: Allure PFP Propyl Cat. #: 969565 Dimensions: 50 x 4.6mm Pore size: 60Å 50mM ammonium phosphate (ph 3.0, adjusted with formic acid) ml/min. Temp.: 35 C UV @ 266nm

Carbonyls by CARB Method 004 on Allure AK Aldehydes, Ketones new! 69 dinitrophenylhydrazine (DNPH) derivatives of aldehydes/ketones Inj.: 0µL Conc.: 3µg/mL each analyte, as aldehyde/ketone Sample diluent: acetonitrile Column: Allure AK Cat.#: 959525-700 Dimensions: 200 x 4.6mm Pore size: 60Å A) water : B):acetonitrile Time (min.) %B 0 60 8 70 0 00.5mL/min. Temp.: 30 C UV @ 360nm Aldehydes and Ketones (DNPH derivatives) on Ultra C8 (40 C) DNPH derivatives of:. formaldehyde 2. acetaldehyde 3. acetone 4. acrolein 5. propionaldehyde 6. crotonaldehyde 7. MEK 8. methacrolein 9. butyraldehyde 0. benzaldehyde. valeraldehyde 2. m-tolualdehyde 3. hexanaldehyde 2 3 4 5 LC_EV0393 Ret. Time Peak (min.) DNPH derivative of:. formaldehyde 4.74 2. acetaldehyde 5.78 3. acrolein 6.86 4. acetone 7.09 5. propionaldehyde 7.3 6. crotonaldehyde 8.9 7. methacrolein 8.55 8. butyraldehyde 8.79 9. methylethylketone 9.06 0. benzaldehyde 0.03. valeraldehyde 0.39 2. m-tolualdehyde.08 3. hexaldehyde.36 Inj.: 0µL Conc.: 3.0µg/mL each derivative Column: Ultra C8 Cat. #: 974565 Dimensions: 50 x 4.6mm Pore size: 00Å Solvent A: water Solvent B: acetonitrile Solvent C: tetrahydrofuran 50-30% A/30-65% B/20-5% C, 0-5 min. Flow rate:.5ml/min. UV @ 365nm Temp.: 40 C HPLC APPLICATIONS BIOCHEMICAL/CLINICAL 6 8 7 9 0 2 3 0 2 4 6 8 0 2 4 min. LC_0036

70 Explosives EPA 8330B Explosives on Ultra C8 HPLC APPLICATIONS ENVIRONMENTAL Ret. Time (min.). HMX 3.38 2. RDX 5.4 3.,3,5-TNB 7.39 4.,3-DNB 8.82 5. 3,5-DNA 9.7 6. NB 9.7 7. tetryl 9.88 8. 2,4,6-TNT.69 9. NG.69 0. 2-A-4,6-DNT 2.05. 4-A-2,6-DNT 2.6 2. 2,4-DNT 3.27 3. 2,6-DNT 3.64 4. 2-NT 5.92 5. 4-NT 7.05 6. 3-NT 8.32 7. PETN 2.08 EPA 8330B Explosives on Pinnacle II Biphenyl Ret. Time (min.). HMX 2.76 2. RDX 3.22 3. NG 4.94 4. 3,5-DNA 5.73 5. NB 6.3 6. 4-A-2,6-DNT 7.02 7. 2-A-4,6-DNT 7.43 8.,3-DNB 9.26 9. 2-NT 9.73 0. PETN 0.43. 3-NT.07 2. 4-NT.07 3. 2,6-DNT 2.36 4.,3,5-TNB 4.54 5. 2,4-DNT 5.46 6. tetryl 6.2 7. 2,4,6-TNT 20.7 Column: Ultra C8 Cat.#: 974575 Dimensions: 250mm x 4.6mm Pore size: 00Å 50µg/mL each compound diluted in acetonitrile 8330 Calibration Mix # (cat.# 3450) 8330 Calibration Mix #2 (cat.# 345) PETN Standard (cat.# 3600) 3,5-dinitroaniline Standard (cat.# 366) Nitroglycerin Standard (cat.# 3498) Inj.: 0µL water:methanol (44:56 v/v).0ml/min. Temp.: 30 C UV detection @ 20nm Column: Pinnacle II Biphenyl Cat.#: 9209565-700 Dimensions: 250mm x 4.6mm Pore size: 0Å LC_EV0428 50µg/mL each compound diluted in acetonitrile 8330 Calibration Mix # (cat.# 3450) 8330 Calibration Mix #2 (cat.# 345) PETN Standard (cat.# 3600) 3,5-dinitroaniline Standard (cat.# 366) Nitroglycerin Standard (cat.# 3498) Inj.: 0µL water:methanol (44:55 v/v).2ml/min. Temp.: 30 C UV detection @ 20nm LC_EV0429

Herbicides, Pesticides 7 Herbicides (Phenylurea) on Ultra C8 Herbicides on Ultra Aqueous C8. 2,4-D 2. Silvex (2,4,5-TP) 3.,4-dichlorobenzene (IS) Fast isocratic analysis! Conc. (µg/ml or ppm). tebuthiuron 2 2. thidiazuron 2 3. monuron 5 4. fluometuron 2 5. diuron 2 6. propanil 2 7. siduron 2 8. linuron 2 9. carbazole 5 0. diflubenzuron 2 2 Inj.: 0µL Conc.: 0ppm each Solvent: 0.05% phosphoric acid : acetonitrile, 50:50 Column: Ultra Aqueous C8 Cat. #: 978355 Dimensions: 50 x 4.6mm Particle size: 3µm Pore size: 00Å 0.05% phosphoric acid : acetonitrile, 60:40.0mL/min. Temp.: ambient UV @ 225nm 3 LC_039 Inj.: 20µL Conc.: see peak list Sample diluent: acetonitrile Column: Ultra C8 Cat. #: 974565 Dimensions: 50 x 4.6mm Pore size: 00Å A: 25mM phosphate buffer, ph 2.5 B: acetonitrile Time (min.) %B 0 40 8 40 4 55 8 55 9 40.5 ml/min. Temp.: ambient UV @ 245nm Diflubenzuron (Pesticide) on Ultra IBD. diflubenzuron 2. N,N'-bis-(chlorobenzyl)urea Inj.: 0µL Conc.: mg/ml Solvent: water:acetonitrile (:, v/v) Column: Ultra IBD Cat. #: 975365 Dimensions: 50 x 4.6mm Particle size: 3µm Pore size: 00Å Solvent A: Flow rate: Temp.: water:acetonitrile (45:55, v/v) 65% 0.M ammonium acetate ph 6.0.0mL/min. ambient UV @ 260nm 2 HPLC APPLICATIONS ENVIRONMENTAL 0 2 4 6 8 0 2 4 min. LC_0304 0 2 4 6 8 0 2 4 min. LC_008

72 Herbicides Phenoxyacid Herbicides on Ultra Aqueous C8 new! HPLC APPLICATIONS ENVIRONMENTAL Mix A and Mix B. picloram 2. chloramben 3. 4-nitrophenol 4. dicamba 5. bentazon 2,4-dichlorophenyl acetic acid (surr.) (not included) 7. 2,4-D 8. MCPA 9. 3,5-dichlorobenzoic acid 0. MCPP. dichlorprop 2. 2,4,5-T 3. 2,4-DB 4. 2,4,5-TP (Silvex) 5. acifluorfen,4-dichlorobenzene (int. std.) (not included) 7. dinoseb 8. pentachlorophenol Phenoxyacid Herbicides on Allure Basix Mix A and Mix B. picloram 2. chloramben 3. 4-nitrophenol 4. dicamba 5. bentazon 2,4-dichlorophenyl acetic acid (surr.) (not included) 7. 2,4-D 8. MCPA 9. 3,5-dichlorobenzoic acid 0. MCPP. dichlorprop 2. 2,4,5-T 3. 2,4-DB 4. 2,4,5-TP (Silvex) 5. acifluorfen,4-dichlorobenzene (int. std.) (not included) 7. dinoseb 8. pentachlorophenol Chlorinated Acids by HPLC, Mix A (cat.# 3243), or Chlorinated Acids by HPLC, Mix B (cat.# 32430) 000µg/mL each component in acetonitrile Inj.: 0µL Conc.: 0ppm each analyte Sample diluent: acetonitrile Column: Ultra Aqueous C8 Cat.#: 978565 Dimensions: 50 x 4.6mm Pore size: 00Å Temp.: LC_EV0356 LC_EV0355 A) 0.05% H3PO4 B) acetonitrile Time (min.) %B 0 20.0 28 80.0 33 90.0 34 20.0.0mL/min. ambient UV @ 225nm new! Chlorinated Acids by HPLC, Mix A (cat.# 3243), or Chlorinated Acids by HPLC, Mix B (cat.# 32430) 000µg/mL each component in acetonitrile Inj.: 0µL Conc.: 0ppm each analyte Sample diluent: acetonitrile Column: Allure Basix Cat.#: 96565 Dimensions: 50 x 4.6mm Pore size: 60Å Temp.: A) 0.05% H3PO4 B) acetonitrile Time (min.) %B 0 20.0 28 80.0 33 90.0 34 20.0.0mL/min. ambient UV @ 225nm LC_EV0357 LC_EV0358

Herbicides, Pesticides 73 Herbicides on Allure C8 Ret. Time (min.). cyanazine 2.04 2. methabenzthiazuron 3.88 3. chlortoluron 4.59 4. secbumeton 5.74 5. isoproturon 6.8 6. diuron 6.78 7. linuron 22.48 8. flusilazole 24.29 9. ethofumesate 24.53 0. neburon 25.74. pendimethalin 3.46 LC_0309 Pesticides (Carbamates) on Ultra Carbamate Compound Ion Cone V. aldicarb sulfone 223.3 25V 2. aldicarb sulfoxide 207.3 8V 3. oxamyl 237.2* 0V 4. methomyl 63.2 5V 5. 3-hydroxycarbofuran 238.3 5V *Ammonium adduct (all other are [M+H] + ions) 3-hydroxycarbofuran oxamyl methiocarb aldicarb sulfone carbofuran propoxur aldicarb sulfoxide carbaryl Compound Ion Cone V 6. aldicarb 9.2 8V 7. propoxur 20.2 8V 8. carbofuran 222.3 22V 9. carbaryl 202.2 8V 0. methiocarb 226.3 9V Inj.: Conc.: Diluent: 5µL custom mix approx. 20µg/mL each acetonitrile Column: Allure C8 Cat. #: 964565 Dimensions: 50 x 4.6mm Pore size: 60Å Temp.: A: water B: acetonitrile Time (min.) %B 0 20 0 40 5 40 26 80 34 80 34. 20.2mL/min. ambient UV @ 220nm Inj.: 0µL Conc.: 00µg/mL each component Column: Ultra Carbamate Cat. #: 97735 Dimensions: 00 x 4.6mm Particle size: 3µm Pore size: 00Å Temp.: MSD Detector: Mode: Capillary V: 3.50 Extractor: 4.0 Ion energy: 0.4 Multiplier: 650 Source temp: 00 C A: 90% water: 0% methanol with 0mM ammonium formate B: 90% acetonitrile: 0% methanol with 0 mm ammonium formate Time (min.) %B 0.0 0% 5 90% 20 90%.0mL/min. (approx. 0.75mL/min. to UV detector, 0.25mL/min. to MSD) ambient UV @ 220nm; MSD Micromass ZMD ESI+ Desolv. temp: 250 C Gas flow: 490 L/hr. HPLC APPLICATIONS ENVIRONMENTAL aldicarb methomyl UV @220nm LC_0308

74 Pesticides Pesticides on Allure C8 HPLC APPLICATIONS ENVIRONMENTAL. desisopropyl atrazine 2. desethyl atrazine 3. simazine 4. terbuthylazine desethyl 5. cyanazine 6. methabenzthiazuron 7. chlortoluron 8. atrazine 9. secbumeton 0. isoproturon. diuron 2. terbumeton 3. terbuthylazine 4. linuron 5. flusilazole 6. ethofumesate 7. neburon Pesticides on Allure PFP Propyl. desisopropyl atrazine 2. desethyl atrazine 3. simazine 4. terbuthylazine desethyl 5. cyanazine 6. atrazine 7. methabenzthiazuron 8. secbumeton 9. chlortoluron 0. isoproturon. terbumeton 2. diuron 3. terbuthylazine 4. linuron 5. ethofumesate 6. flusilazole 7. neburon LC_030 Inj.: 5µL Conc.: approx. 0ppm each pesticide Solvent: acetonitrile Column: Allure C8 Cat. #: 964565 Dimensions: 50 x 4.6mm Pore size: 60Å Mobile phase A: 20mM K2HPO4, ph 7.0 B: acetonitrile Time (min.) %B 0 20 0 40 5 40 30 70 30. 20.2mL/min. Temp.: ambient UV @ 220nm Inj.: 5µL Conc.: approx. 0ppm each pesticide Solvent: acetonitrile Column: Allure PFP Propyl Cat. #: 969355 Dimensions: 50 x 4.6mm Particle size: 3µm Pore size: 60Å Mobile phase A: 20mM K2HPO4, ph 7.0 B: acetonitrile Time (min.) %B 0 20 0 30 2 30 8 50 26 50 26. 20.0 ml/min. Temp.: ambient UV @ 220nm LC_032

Herbicides 75 Paraquat and Diquat on Ultra Quat 2 Peak time (min.). diquat 7.5 2. paraquat 8.8 min. diquat 308nm 00µg/mL 40µg/mL 20µg/mL Paraquat and Diquat on Ultra Quat Fast, sensitive LC/MS/MS analysis! paraquat dichloride [CAS# 90-42-5] diquat dibromide [CAS # 85-00-7] LC_EV0383 Inj.: 20µL Conc.: 20ppm each component (above); 20µg/mL, 40µg/mL, and 00µg/mL each component at 308nm (left) and at 257nm (right) Sample diluent: water Column: Ultra Quat Cat. #: 98565 Dimensions: 50 x 4.6mm Ultra Quat Reagent Solution (cat.# 3244): acetonitrile, 95:5 (v/v).0 ml/min. Temp.: 27 C Peak List. diquat 2. paraquat *Data courtesy of Houssain El Aribi, Ph.D., LC/MS Product and Application Specialist, MDS SCIEX, 7 Four Valley Drive, Concord, Ontario, Canada, L4K 4V8 UV @ 257nm (paraquat) UV @ 308nm (diquat) 00µg/mL 40µg/mL 20µg/mL Inj.: 0µL Conc.: 5µg/mL each component Sample diluent: DI water Sample temp.: ambient Column: Ultra Quat Cat.#: 58352 Dimensions: 50 x 2.mm Particle size: 3µm Pore size: 00Å paraquat 257nm LC_032 0mM heptafluorobutyric acid:acetonitrile, 95:5 (v/v) 0.3mL/min. Temp.: ambient Applied Biosystems API 3200 Interface: electrospray Ion Mode: positive Temp.: 600 C Ion Spray voltage: 5500V Collision exit potential: 3V Curtain Gas : 5psi (03kPa) Gas supply : 70psi (483kPa) Gas supply 2: 60psi (44kPa) Quantitation: (MRM) Q/Q3: unit resolution Dwell time: 200 ms Precursor Ion Fragment Ion DP Collision Energy (amu) (amu) (V) (ev) diquat, 83+ 57+ 30 30 paraquat, 93 (2+) 7+ 20 20 min. new! HPLC APPLICATIONS ENVIRONMENTAL

76 Polynuclear Aromatic Hydrocarbons Polynuclear Aromatic Hydrocarbons (PAHs) on Pinnacle II PAH 6 HPLC APPLICATIONS ENVIRONMENTAL Inj.: 5µL Conc.: see peak list Sample Diluent: ::2 methylene chloride: methanol:acetonitrile Sample Temp.: ambient Column: Pinnacle II PAH Cat.#: 929463 Dimensions: 50mm x 3.2mm Particle size: 4µm Pore size: 0Å A: purified water B: acetonitrile Time (min.) %B 0 40 7 60 00 8 00 Temp.:.2mL/min. ambient UV @ 254nm 2 0 2 4 6 8 0 2 4 6 Time (min) 3 4 5 7 8 9 0 2 3 4 5 6 Conc. (µg/ml). naphthalene 500 2. acenaphthylene 500 3. acenaphthene 500 4. fluorene 500 5. phenanthrene 250 6. anthracene 500 7. fluoranthene 250 8. pyrene 250 9. benzo(a)anthracene 250 0.chrysene 250. benzo(b)fluoranthene 250 2. benzo(k)fluoranthene 250 3. benzo(a)pyrene 250 4. dibenzo(a,h)anthracene 250 5. benzo(ghi)perylene 250 6. indeno(,2,3-cd)pyrene 250 LC_EV0442 free literature Environmental HPLC Applications Columns Reference Materials Restek HPLC columns support environmental HPLC applications with rapid analysis times and effective analyte resolution. Sample turn-around can be 50% faster, or greater, than with alternative columns. In addition, we prepare analytical reference materials and sample preparation products for these applications. Analyses described in this 8-page publication include carbamates, carbonyls, explosives, paraquat/diquat, phenoxyacid herbicides, and polyaromatic hydrocarbons. Call Restek at 800-356-688 or 84-353-300, ext. 5, or contact your Restek representative, to request your free copy! lit. cat.# 5974A (domestic) lit. cat.# 5974A-INT (international)

Dietary Supplements: Plant Extracts 77 Allicin in Garlic Tablets on Pinnacle II C8 Phenethyl Glucosinolate on Ultra Aqueous C8. allicin Inj.: 20µL Conc.:.2mg/mL garlic tablet in water Solvent: deionized water Column: Pinnacle II C8 Cat. #: 924565 Dimensions: 50 x 4.6mm Pore size: 0Å water:methanol (50:50 v/v) 0.8 ml/min. Temp.: 30 C UV @ 240nm 0 2 4 6 8 min. LC_074 Phenolics in Echinacea on Pinnacle II C8 Inj.: 0µL Conc.: 6.7 mg/ml Echinacea capsule contents in sample diluent Solvent: ethanol:water (70:30, v/v) Sample temp.: 25 C Column: Pinnacle II C8 Cat. #: 924565 Dimensions: 50 x 4.6mm Pore size: 0Å A = 0.% phosphoric acid B = acetonitrile Time (min.) %B 0 0 3 22 4 40 4.5 40 5 0 20 0.5mL/min. Temp.: 35 C UV @ 330nm. caftaric acid 2. chlorogenic acid 3. echinacoside 4. cichoric acid. phenethyl glucosinolate Inj.: 0µL Conc.: 000µg/mL Solvent: water Column: Ultra Aqueous C8 Cat. #: 978565 Dimensions: 50 x 4.6mm Pore size: 00Å Temp.: LC_066 3 A: 50mM potassium phosphate, ph 2.5 B: acetonitrile Time (min.): % B 0.0 0 0 75 0 6 0.0mL/min. ambient UV @ 20nm Tocopherols in Soy Oil on Pinnacle II Silica. α-tocopherol 2. β-tocopherol 3. γ-tocopherol 4. δ-tocopherol Inj.: 0µL Conc.: approx..25% soy oil Solvent: hexane Column: Pinnacle II Silica Cat. #: 920565 Dimensions: 50 x 4.6mm Pore size: 0Å isopropyl alcohol:hexane (0.5:99.5) 0.6 ml/min. Temp.: 30 C UV @ 295nm HPLC APPLICATIONS NUTRACEUTICAL 4 2 LC_095 0 2 4 6 8 0 2 4 min. LC_097

78 Preservatives, Vitamins Parabens on Pinnacle II C8 Parabens on Pinnacle II Phenyl Column: Pinnacle II C8 Cat. #: 923565 Dimensions: 50 x 4.6mm Pore size: 0Å Column: Pinnacle II Phenyl Cat. #: 925565 Dimensions: 50 x 4.6mm Pore size: 0Å HPLC APPLICATIONS FOODS & FLAVORS Vitamins (Fat Soluble) on Ultra C8 Conc.. (mg/ml). solvent front n/a 2. menadione (vitamin K3) 0.45 3. all-trans-retinol (vitamin A) 0.34 4. vitamin D3 0.4 5. unknown n/a Solvent: diethyl ether 6. α tocopherol (vitamin E) 2.4 7. α tocopherol acetate (vitamin E acetate) 2.4 Column: Cat. #: Ultra C8 974575 8. unknown n/a Dimensions: 250 x 4.6mm 9. phylloquinone (vitamin K) 0.84 Pore size: 00Å 2 3 LC_076 4 acetonitrile:methanol (90:0, v/v) Time (min.) Flow (ml/min.).00.00 5.00.00 5.0 2.00 50.0 2.00 Temp.: 30 C UV @ 280nm Conc. (µg/ml). methyl paraben 0 2. ethyl paraben 20 3. propyl paraben 30 4. butyl paraben 40 Inj.: 5µL Solvent: methanol:water (50:50 v/v) Temp.: 0.% acetic acid in water: acetonitrile (50:50, v/v).0ml/min. ambient UV @ 254nm 0 2 3 4 5 min. Vitamins Thiamin (Vitamin B) and Ascorbic Acid (Vitamin C) on Ultra IBD 2 LC_077 Conc. (mg/ml). thiamin 2.5 2. ascorbic acid 2.5 Inj.: Solvent:.0µL mobile phase Column: Ultra IBD Cat. #: 975565 Dimensions: 50 x 4.6mm Pore size: 00Å Temp.: 3 4 50mM potassium phosphate, ph 2.5.0mL/min. ambient UV @ 254nm 6 7 9 5 8 0 0 20 30 40 min. LC_044 LC_0227

Preservatives, Flavor Compounds 79 Phenolic Antioxidants on Pinnacle II C8 Sorbic and Benzoic Acids on Pinnacle II Phenyl Conc. (ppm). propyl gallate 68 2. TBHQ 82 3. 2-BHA + 3-BHA 97 4. BHT 93 Black Pepper: Piperine on Pinnacle II Cyano. piperine Inj.: 5µL Conc.: 00µg/mL Solvent: methanol Column: Pinnacle II Cyano Cat. #: 926565 Dimensions: 50 x 4.6mm Pore size: 0Å Temp.: 2 0 2 4 6 8 0 2 4 6 8min. LC_098 water:acetonitrile (50:50 v/v).0ml/min. ambient UV/Vis @ 345nm 3 Inj.: 0µL Solvent: methanol Column: Pinnacle II C8 Cat. #: 924565 Dimensions: 50 x 4.6mm Pore size: 0Å 4 A = % acetic acid B = methanol Time B (min.) (%) 0 50 4 50 0 90 25 90 26 50.0mL/min. Temp.: 30 C UV @ 280nm Column: Pinnacle II Phenyl Cat. #: 925565 Dimensions: 50 x 4.6mm Pore size: 0Å % acetic acid:methanol (80:20).0 ml/min. Temp.: 25 C UV @ 230nm 0 2 4 6 8 0 2 4 6 8 min. LC_099 Capsaicinoids: Heat Level Assay for Habeñero Nuggets on Pinnacle II C8. unknown 2. capsaicin 3. dihydrocapsaicin Inj.: 0µL habañero nugget extract Conc.: 0.8g sample in 200mL Solvent: ethanol Column: Pinnacle II C8 Cat. #: 924565 Dimensions: 50 x 4.6mm Pore size: 0Å Temp.: A: % acetic acid in water (v/v) B: acetonitrile Time (min.) % B 0 50 2 50 9 80 2 80 3 50.0mL/min. ambient UV @ 280nm. sorbic acid 2. benzoic acid Inj.: 0µL Conc.: 00ppm each Solvent: methanol 2 HPLC APPLICATIONS FOODS & FLAVORS 0 2 3 4 5 6 7 min. LC_08 LC_060

80 Preservatives, Organic Acids Carboxylic Acids (Pyruvic and Lactic) on Ultra Aqueous C8 Carboxylic Acids on Ultra Aqueous C8 HPLC APPLICATIONS FOODS & FLAVORS Conc. (mg/ml). pyruvic acid 5 2. lactic acid 50 Inj.: 5µL Solvent: 50mM potassium phosphate, ph 2.5 Column: Ultra Aqueous C8 Cat. #: 978565 Dimensions: 50 x 4.6mm Pore size: 00Å 50mM potassium phosphate, ph 2.5.0mL/min. Temp.: 26 C UV @ 20nm LC_0297 Organic Acids on Allure Organic Acids. malic acid 2. isocitric acid restek innovation! Excellent peak symmetry in 00% aqueous mobile phase! Inj.: 5µL Conc.: approx. 0.2 mg/ml malic acid; 0.02 mg/ml isocitric acid Solvent: water Column: Allure Organic Acids Cat. #: 965575 Dimensions: 250 x 4.6mm Pore size: 60Å 00mM KH2PO4, ph 2.5 0.5mL/min. Temp.: ambient UV @ 226nm Conc. (µg/ml):. malonic acid 500 2. lactic acid 500 3. acetic acid 000 4. citric acid 000 5. succinic acid 2000 6. fumaric acid 0 Inj.: 0µL Solvent: water Column: Ultra Aqueous C8 Cat. #: 978565 Dimensions: 50 x 4.6mm Pore size: 00Å 50mM potassium phosphate, ph 2.5: acetonitrile (99:).5mL/min. Temp.: 25 C UV @ 20nm 2 0 2 3 4 min. LC_040 Organic Acids on Allure Organic Acids restek innovation! Baseline resolution of tartaric and quinic acids in 00% aqueous mobile phase! 3 Conc. (mg/ml). tartaric acid 2. quinic acid 3. malic acid 4. citric acid 5. fumaric acid 0.005 Inj.: Solvent: 4 0µL standard solution water Column: Allure Organic Acids Cat. #: 965585 Dimensions: 300 x 4.6mm Pore size: 60Å 00mM phosphate buffer, ph 2.5 0.5mL/min. Temp.: ambient UV @ 226nm 5 6 LC_03 LC_0238

Organic Acids, Sugars, Flavoring 8 Organic Acids in Cranberry Juice on Allure Organic Acids Sugars on Pinnacle II Amino (3μm). quinic acid 2. malic acid 3. citric acid Inj.: 0µL Conc.: cranberry juice:water (50:50, v/v) Solvent: water Column: Allure Organic Acids Cat. #: 965585 Dimensions: 300 x 4.6mm Pore size: 60Å 00mM phosphate buffer, ph 2.5 0.5mL/min. Temp.: ambient UV @ 226nm LC_0236 Sugars: Maple Syrup on Pinnacle II Amino. fructose 2. glucose 3. sucrose 3 Inj.: Conc.: Solvent: 50.0µL % maple syrup in solvent acetonitrile:water (70:30, v/v) Column: Pinnacle II Amino Cat. #: 927565 Dimensions: 50 x 4.6mm Pore size: 0Å water:acetonitrile (25:75).0mL/min. Temp.: 35 C refractive index @ 35 C Conc. (mg/ml). fructose 2.0 2. glucose 2. 3. sucrose 4.0 4. maltose 4.5 5. lactose 4.4 Inj.: 5µL Solvent: mobile phase Column: Pinnacle II Amino Cat. #: 927365 Dimensions: 50 x 4.6mm Particle size: 3µm Pore size: 0Å water:acetonitrile (25:75, v/v).5 ml/min. Temp.: 35 C refractive index @ 35 C LC_0223 Vanilla Bean Extract on Pinnacle DB C8 (Fast LC) Inj.: 5µL Conc.: 20% v/v commercial extract in water Column: Pinnacle DB C8 Cat. #: 944555 Dimensions: 50 x 4.6mm Pore size: 40Å 0.2% v/v phosphoric acid in water:methanol (80:20 v/v) 2mL/min. Temp.: 27 C UV @ 254nm Peak List Ret. Time (min.). vanillin 2.00 HPLC APPLICATIONS FOODS & FLAVORS 2 0 2 4 6 8 0 2 4 min. LC_0205 LC_0252

82 Flavorings, Food Contaminants HPLC APPLICATIONS FOODS & FLAVORS Vanillin on Ultra C8 Inj.: Conc.: Solvent: 0mL 5% solution of vanilla extract 40% ethanol Column: Ultra C8 Cat. #: 903565 Dimensions: 50x4.6mm Pore size: 00Å Mobile phases: A:.2% acetic acid in water B: methanol Time (min.) % B 0.0 20.0 5.0 20.0 5.0 40.0 20.0 40.0 25.0 20.0.0mL/min. Temp.: 28 C UV @ 254nm. vanillin 0 2 4 6 8 0 2 4 6 8 min. LC_049 Sudan Dyes on Ultra Aqueous C8 Photodiode Array Detector Vanillin and Ethyl Vanillin on Ultra C8 Conc. (mg/ml). vanillin 0.2 2. ethyl vanillin 0.04 Inj.: 0µL Solvent: 40% ethanol Column: Ultra C8 Cat. #: 903565 Dimensions: 50 x 4.6mm Pore size: 00Å Mobile phases: A:.2% acetic acid B: methanol Time (min.) %B 0.0 20.0 5.0 20.0 5.0 40.0 20.0 40.0 25.0 20.0.0mL/min. Temp.: 28 C UV @ 254nm 0 2 4 6 8 0 2 4 6 8 min. Fixed Dual Wavelength Detector LC_048 2 new!. Sudan I 2. Sudan II 3. Sudan III 4. Sudan IV wavelength change Monitor Sudan I, II, III, and IV in a single, isocratic analysis! LC_FF0326 LC_FF0327 Inj.: 20µL, mixture of Sudan I, Sudan II, Sudan III, Sudan IV Conc.: 20µg/mL each component Sample diluent: methanol Column: Ultra Aqueous C8 Cat.#: 978565 Dimensions: 50 x 4.6 mm Pore size: 00Å methanol:water, 97:3 ml/min. Temp.: ambient UV @ 476 / 493 / 52 / 357 nm (left) or UV @ 488 / 520 nm (right)

Food Contaminants 83 Nitrofuran Metabolites by LC-MS/MS on Ultra C8 d5-amoz AMOZ Conc. (ppb) Time (min). d5-amoz 0.3 3.7 2. AMOZ 0.3 3.8 3. AHD 0.3 5.46 4. d4-aoz 0.3 5.49 5. AOZ 0.3 5.5 6. SC 0.3 5.6 Column: Ultra C8 Cat. #: 97432 Dimensions: 00 x 2.mm Particle size: 3µm Pore size: 00Å A: 0.05% formic acid in methanol B: 0.05% formic acid 5 mm NH4 formate in water Time (min) %B 0 90 2.5 90 5 0 0 0 2 90 5 90 AHD d4-aoz AOZ LC_0323 200µL/min. Temp.: 30 C MS/MS triple quadrupoles (Thermo Scientific Discovery ) SC Analyzer Parameters: Ion source: ESI (electrospray ionization) Only segment: 5 min. Polarity: positive Data type: centroid Scan mode: SRM product Scan width (m/z): 0.7 Scan time (s): 0.25 Peak width: Q: within 0.7 Q2: 0.7 Collision gas pressure (mtorr):.5 (argon) Divert valve: active, with 3 positions Positions- 2 min., 2 8 min., 3 5 min. Analyte Prec. Ion Prod. Ion Collision E Tube Lens AOZ 236 34 2 V 20 AMOZ 335 29 0 V 00 SC 209 66 2 V 80 AHD 249 34 2 V 0 AMOZ = 3-amino-5-morpholinomethyl-2-oxazolidinone AHD = -aminohydantoin hydrochloride AOZ = 3-amino-2-oxazolidinone SC = semicarbazide HPLC APPLICATIONS FOODS & FLAVORS for more info For more information, review Restek Advantage 2005 vol.2, page, on our website. lit. cat.# 59923

84 Analgesics Hydroxybenzoic Acids on Ultra Aqueous C8 new!. 3,5-dihydroxybenzoic acid 2. 2,5-dihydroxybenzoic acid 3. 4-hydroxybenzoic acid 4. 3-hydroxybenzoic acid 5. 2,4-dihydroxybenzoic acid 6. benzoic acid 7. salicylic acid HPLC APPLICATIONS PHARMACEUTICAL Analgesic Acetaminophen and Narcotic Analgesics on Allure Basix 2. acetaminophen 2. codeine 3. oxycodone 4. hydroxycodone Inj.: 5µL Conc.: 00µg/mL Solvent: mobile phase Column: Allure Basix Cat. #: 96565 Dimensions: 50 x 4.6mm Pore size: 60Å LC_PH0400 20mM ammonium acetate, ph 4.5: acetonitrile (85:5, v/v) Flow rate:.0ml/min. Temp.: 30 C UV @ 280nm Conc. (µg/ml) U. unknown unknown. morphine sulfate 204 2. acetaminophen 92 3. codeine phosphate 26 4. oxycodone HCl 206 5. hydrocodone bitartrate 28 Inj.: 5µL Conc.: 50µg/mL each component Sample diluent: mobile phase Column: Ultra Aqueous C8 Cat.#: 978565 Dimensions: 50 x 4.6mm Pore size: 00Å A: 20mM potassium phosphate (ph 2.5); B: acetonitrile Time (min.) %B 0.00 20 5.00 50 0.00 50.0mL/min. Temp.: ambient UV @ 20nm Analgesic Acetaminophen and Narcotic Analgesics on Ultra C8 Inj.: Solvent: 4.0µL raw material mix mobile phase Column: Ultra C8 Catalog #: 974575 Dimensions: 250 x 4.6mm Pore size: 00Å A: 0mm potassium phosphate, ph 2.8 B: acetonitrile:methanol, (90:0, v/v) (85A:5B, v/v).0ml/min. Temp.: 35 C UV @ 235nm 3 4 0 2 3 4 5 6 7 8 9 min. LC_0082 LC_022

Analgesics, Antiarrhythmics 85 Narcotic Analgesic Morphine Sulfate Raw Material - USP 25: Resolution Solution on Pinnacle II C8 Conc. (µg/ml). unknown unknown 2. phenol 0.5 3. morphine sulfate 0.24 Inj.: Solvent: Column: Pinnacle II C8 Cat. #: 924575-700 Dimensions: 250 x 4.6mm Pore size: 0Å Temp.: 20.0µL mobile phase Column: Ultra C8 Cat. #: 903564 Dimensions: 50 x 4.0mm Pore size: 00Å A: 0.73g -heptane sulfonic acid and 0mL glacial acetic acid, diluted to 720mL with water, ph=2.33 B: methanol (72A:28B, v/v).00ml/min, 26.5 C UV @ 284nm LC_0209 Narcotic Analgesic Oxycodone HCl Raw Material by USP 25: Resolution Solution on Ultra C8 Conc. (µg/ml) Ret. Time (min.) RRT Tailing Res.. codeine phosphate 3 9.3 0.7. 2. oxycodone HCl 9 26.8.0 0.99 3.3 Inj.: 0µL resolution solution Solvent: mobile phase A: 5mM hexane sulfonic acid with 2mL TEA and 3mL of phosphoric acid per liter. ph adjusted to 2.5 B: methanol (90A:0B, v/v).5ml/min. Temp.: 40 C UV @ 206nm Narcotic Analgesic Hydrocodone Bitartrate by USP 25 on Pinnacle II Silica Ret. Time (min.) Inj. % RSD (n=5). hydrocodone bitartrate 3. 0.37% Inj.: 20µL Conc.: µg/ml assay standard Solvent: mobile phase Column: Pinnacle II Silica Cat. #: 920575 Dimensions: 250 x 4.6mm Pore size: 0Å.5mL/min. Temp.: 27 C UV @ 280nm Impurities resolved from primary peak. A: 800mL acetonitrile; 4mL water; ml diethylamine B: methanol (55A:45B, v/v) 0 2 3 4 min. LC_02 Antiarrhythmic Calcium Channel Blockers on Ultra Cyano. diltiazem 2. nifedipine impurity 3. verapamil 4. nifedipine 5. nicardipine Inj.: 5µL Conc.: 00mg/mL Solvent: acetonitrile:water (:) Column: Ultra Cyano Cat. #: 906565 Dimensions: 50 x 4.6mm Pore size: 00Å Flow rate: Temp.: 20mM potassium phosphate monobasic, ph 3.0:acetonitrile (70:30, v/v).2ml/min. 30 C (± C) 235nm 4 5 HPLC APPLICATIONS PHARMACEUTICAL 3 2 LC_023 0 2 3 4 5 6 7 8 9 min. LC_0062

86 Antiarrhythmics Digitalis Extracts/Derivatives on Ultra PFP Propyl Cartridge (Fast LC) Antiarrhythmic Furosemide on Ultra IBD HPLC APPLICATIONS PHARMACEUTICAL Conc. Ret. Time (min.). digoxigenin 00µg/mL 0.40 2. gitoxigenin 00µg/mL 0.80 3. digoxin 00µg/mL.0 4. gitoxin ~0µg/mL 2.20 5. digitoxin 00µg/mL 2.60 Inj.: 0µL Solvent: water:acetonitrile (80:20 v/v) Column: Ultra PFP Propyl Cartridge Dimensions: 30 x 4.0mm Particle size: 3µm Pore size: 00Å A: water B: acetonitrile Time (min.) %B 0.0 20.5 20.5 35 3.0 35 3. 20 2.0mL/min. Temp.: 27 C UV @ 230nm LC_0250 Antiarrhythmic Atenolol on Allure Basix 2. uracil (marker) 2. atenolol Inj.: µl Conc.: mg/ml Solvent: water:methanol (7:3, v/v) Column: Allure Basix Cat. #: 96565 Dimensions: 50 x 4.6mm Pore size: 60Å acetonitrile:20mm ammonium acetate, ph 4.5 (80:20, v/v).2ml/min. Flow rate: Temp.: 25 C UV @ 225nm. uracil (marker) 2. furosemide Inj.: 5µL Conc.: 000µg/mL Solvent: acetonitrile:water (60:40, v/v) Column: Ultra IBD Cat. #: 975565 Dimensions: 50 x 4.6mm Pore size: 00Å 20mM ammonium acetate, ph 4.5: acetonitrile (70:30, v/v) Flow rate: 2.0mL/min. Temp.: 27 C UV @ 280nm LC_007 Impurities in Furosemide (enlargement of baseline) Antiarrhythmic Enalapril Maleate on Allure Basix. maleate 2. enalapril Inj.: Conc.: Solvent: 2.5µL.mg/mL methanol:water (30:70) Column: Allure Basix Cat. #: 96565 Dimensions: 50 x 4.6mm Pore size: 60Å 0mM ammonium formate, ph 2.5: acetonitrile (20:80, v/v) Flow rate:.2ml/min. Temp.: 25 C UV @ 225nm 0 2 4 6 8 0 2 min. LC_0072 LC_009

Antiarrhythmics 87 Antiarrhythmic Timolol Maleate on Allure Basix Antiarrhythmic Metoprolol on Allure Basix. maleate 2. timolol Inj.: 2.5µL Conc.:.2mg/mL Solvent: methanol:water (30:70) Column: Allure Basix Cat. #: 96565 Dimensions: 50 x 4.6mm Pore size: 60Å 0mM ammonium formate, ph 2.5: acetonitrile (20:80, v/v) Flow rate:.2ml/min. Temp.: 25 C UV @ 290nm LC_0092 Antiarrhythmic Triamterene and Hydrochlorothiazide on Allure Basix. hydrochlorothiazide 2. triamterene Inj.: µl Conc.: 500µg/mL Solvent: methanol:water: tetrahydrofuran (30:35:35) Column: Allure Basix Cat. #: 96565 Dimensions: 50 x 4.6mm Pore size: 60Å 20mM ammonium acetate, ph 4.5:acetonitrile (65:35,v/v) Flow rate:.2ml/min. Temp.: 25 C UV @ 225nm. unknown 2. metoprolol Inj.: Conc.: Solvent: 2.5µL.5mg/mL methanol:water (30:70) Column: Allure Basix Cat. #: 96565 Dimensions: 50 x 4.6mm Pore size: 60Å 0mM ammonium formate, ph 2.5: acetonitrile (0:90, v/v) Flow rate:.2ml/min. Temp.: 25 C UV @ 225nm LC_0090 Antiarrhythmic Verapamil HCl on Allure Basix. toluene (marker) 2. verapamil HCl Inj.: µl Conc.: mg/ml Solvent: water:acetonitrile (70:30, v/v) Column: Allure Basix Cat. #: 96565 Dimensions: 50 x 4.6mm Pore size: 60Å acetonitrile: 20mM ammonium acetate, ph 4.5 (65:35,v/v) Flow rate:.2ml/min. Temp.: 25 C UV @ 230nm 2 HPLC APPLICATIONS PHARMACEUTICAL 0 2 3 4 5 6 7 8 9 min. LC_0086 LC_0077

88 Antiasthmatics, Antibiotics Antiasthmatic Beclomethasone on Ultra C8 Antibiotic Amoxicillin on Ultra IBD HPLC APPLICATIONS PHARMACEUTICAL. beclomethasone 2. deschlorobeclomethasone Inj.: 0µL Conc.: 79.5µg/mL (beclomethasone) Solvent: water:acetonitrile (0:90, v/v) Column: Ultra C8 Cat. #: 974565 Dimensions: 50 x 4.6mm Pore size: 00Å water:acetonitrile (70:30, v/v).0ml/min. Temp.: 25 C UV @ 238nm 0 5 0 5 20 min. LC_020 Antibiotic Cephalexin on Ultra IBD 2. amoxicillin Inj.: 5µL Conc.:.5mg/mL Solvent: acetonitrile:water (0:90, v/v) Column: Ultra IBD Cat. #: 975565 Dimensions: 50 x 4.6mm Pore size: 00Å 0mM ammonium formate, ph 2.5: acetonitrile (95:5, v/v) Flow rate:.2ml/min. Temp.: 30 C UV @ 270nm 0 2 3 4 5 6 min. LC_0095 Antibiotic Penicillin V on Ultra IBD. cephalexin 2. cephradine Inj.: 0µL Conc.: 500µg/mL Solvent: acetonitrile:water (0:90, v/v) Column: Ultra IBD Cat. #: 975565 Dimensions: 50 x 4.6mm Pore size: 00Å 0mM ammonium formate, ph 2.5: acetonitrile (90:0, v/v) Flow rate:.2ml/min. Temp.: 30 C UV @ 270nm 2. penicillin V Inj.: Conc.: Solvent: 2.5µL.2mg/mL acetonitrile:water (0:90,v/v) Column: Ultra IBD Cat. #: 975565 Dimensions: 50 x 4.6mm Pore size: 00Å 0mM ammonium formate, ph 2.5: acetonitrile (95:5, v/v) Flow rate:.2ml/min. Temp.: 30 C UV @ 270nm 0 2 4 6 8 0 min. 0 2 3 4 5 6 7 8 min. LC_0094 LC_0096

Antibiotics, Antidepressants 89 Antibiotic Sulfamethoxazole on Allure Basix. sulfamethoxazole Inj.: 0µL Conc.: 00µg/mL Solvent: water:methanol (:, v/v) Column: Allure Basix Cat. #: 96565 Dimensions: 50 x 4.6mm Pore size: 60Å Flow rate: Temp.: water:methanol (70:30, v/v).0ml/min. ambient UV @ 254nm 0 2.5 5 7.5 0 2.5 5 7.5 min. LC_0066 Antibiotic Cephaloridine on Ultra IBD (Reversed Phase). cephaloridine Inj.: 5µL Conc.: mg/ml Solvent: acetonitrile:water (50:50, v/v) Column: Ultra IBD Cat. #: 975565 Dimensions: 50 x 4.6mm Pore size: 00Å acetonitrile: 20mM ammonium phosphate, ph 4.0 (20:80,v/v).2mL/min. Temp.: 27 C UV @ 254nm Antibiotic Sulfamethoxazole, Trimethoprim, and Naphthoate on Allure C8 2 3. trimethoprim 2. sulfamethoxazole 3. naphthoate Inj.: 0µL Conc.: 00µg/mL Solvent: water:methanol (:, v/v) Column: Allure C8 Cat. #: 964565 Dimensions: 50 x 4.6mm Pore size: 60Å Flow rate: Temp.: 0mM ammonium formate, ph to 3.0 with formic acid: methanol (50:50, v/v).0ml/min. ambient UV @ 220nm 0 2 4 6 8 0 2 4 min. LC_0067 Antidepressants (Tricyclic) on Allure Basix. doxepin 2. nordoxepin 3. amitriptyline 4. imipramine 5. desimipramine 6. nortriptyline 7. protriptyline 2 3 Inj.: 0µL Conc.: 00µg/mL each Solvent: mobile phase Column: Allure Basix Cat. #: 96565 Dimensions: 50 x 4.6mm Pore size: 60Å acetonitrile: 50mM KH2PO4 ph3:methanol (60:25:5, v/v) Flow rate:.2ml/min. Temp.: 30 C UV @ 254nm 4 5 6 HPLC APPLICATIONS PHARMACEUTICAL 7 0 2 3 4 5 6 7 8 min. 0 2 3 4 5 6 7 8 9 min. LC_0053 LC_002

90 Selective Serotonin Reuptake Inhibitors (SSRIs) HPLC APPLICATIONS PHARMACEUTICAL Tetracycline Mix on Allure Biphenyl Ret. Time Peak List (min.). oxytetracycline 4.59 2. degradation peak 5.9 3. tetracycline 6.32 4. degradation peak.6 5. chlortetracycline 5.8 6. doxycycline 9.66 Inj.: 30µL Conc.: 00µg/mL each component Sample diluent: methanol Column: Allure Biphenyl Cat. #: 966565 Dimensions: 50 x 4.6 mm Pore size: 60Å Temp.: 20mM ammonium phosphate (ph 2.5):acetonitrile, 80:20 ml/min. ambient UV @ 254 nm LC_PH0347 Selective Serotonin Reuptake Inhibitors (SSRIs) on phidelity C8 Oxytetracycline and Tetracycline on Allure Biphenyl Ret. Time Peak List (min.). oxytetracycline 4.92 2. degradation peak 5.66 3. tetracycline 7.0 Inj.: 20µL Conc.: 00µg/mL each component Sample diluent: methanol Column: Allure Biphenyl Cat. #: 966565 Dimensions: 50 x 4.6 mm Pore size: 60Å Temp.: 20mM ammonium phosphate (ph 2.5):acetonitrile, 80:20 ml/min. ambient UV @ 254 nm Peak List. uracil (marker) 2. fluvoxamine maleate 3. fluoxetine 4. sertraline HCl Inj.: 0µL Conc.: 00µg/mL each component Sample diluent: acetonitrile Column: phidelity C8 Cat.#: 9579365 Dimensions: 50 x 4.6 mm Particle size: 3µm Pore size: 200Å LC_PH0340 new! 0mM ammonium bicarbonate (ph ): acetonitrile:tetrahydrofuran, 45:45:0.5mL/min. Temp.: ambient UV @ 230 nm LC_PH036

Anti-Diabetic, Anti-Ulcerative, Bronchodilator, Corticosteroids 9 Anti-Diabetic Glyburide (Glybenclamide) on Ultra IBD 2 Anti-Ulcerative Cimetidine on Ultra IBD (LC/MS). unknown 2. glyburide Inj.: µl Conc.: 500mg/mL Solvent: water:methanol:acetone (::2, v/v) Column: Ultra IBD Cat. #: 975565 Dimensions: 50 x 4.6mm Pore size: 00Å water with 0.% (v/v) acetic acid: acetonitrile (95:05, v/v) Flow rate:.0ml/min. Temp.: 27 C UV @ 254nm 0 2 3 4 5 min. LC_0069 Bronchodilator Albuterol on Allure Basix. albuterol Inj.: Conc.: Solvent: 2.5µL.mg/mL methanol:water (30:70) Column: Allure Basix Cat. #: 96565 Dimensions: 50 x 4.6mm Pore size: 60Å 0mM ammonium formate, ph 2.5: acetonitrile (20:80, v/v) Flow rate:.2ml/min. Temp.: 25 C UV @ 225nm. cimetidine Inj.: 5µL Conc.: 0µg/mL Solvent: mobile phase Column: Ultra IBD Cat. #: 975552 Dimensions: 50 x 2.mm Pore size: 00Å water:acetonitrile (20:80, v/v) 0.2 ml/min. Column temp.: ambient PE/Sciex API 50 EX Interface: turbo ion spray Ion mode: positive Ion spray voltage: 4800V Temp.: 350 C Orifice: 0V Ring: 00V Auxiliary gas: 6000cc/min. 0 0.2 0.4 0.6 0.8.0.2.4.6.8 min. LC_05 Corticosteroids on Pinnacle II Cyano (Reversed Phase) Conc. (mg/ml). cortisone 0. 2. corticosterone 0.5 3. cortisone acetate 0.7 Inj.: Solvent: 5.0µL methanol Column: Pinnacle II Cyano Cat. #: 926565 Dimensions: 50 x 4.6mm Pore size: 0Å water:methanol (65:35, v/v).0ml/min. Temp.: 30 C UV @ 254nm Pressure: 33 bar 2 3 HPLC APPLICATIONS PHARMACEUTICAL 0 2 3 4 5 min. LC_0089 0 2 3 4 5 6 7 8 9 min. LC_086

92 Corticosteroids Corticosteroids on Ultra C8 Corticosteroids on Allure Biphenyl HPLC APPLICATIONS PHARMACEUTICAL. uracil 2. triamcinolone 3. hydrocortisone 4. dexamethasone 5. corticosterone 6. deoxycorticosterone Inj.: 5µL Conc.: 00µg/mL Solvent: mobile phase Column: Ultra C8 Cat. #: 974565 Dimensions: 50 x 4.6mm Pore size: 00Å water:methanol (30:70, v/v).0ml/min. Temp.: 30 C UV @ 220nm 2 0 2 3 4 5 6 min. LC_008 Corticosteroids on Allure Biphenyl 3 4 5 6 Ret. Time Peak List (min.). hydrocortisone 4.9 2. prednisone 4.79 3. cortisone 5.08 4. dexamethasone 6.37 5. corticosterone 9.0 6. cortisone acetate 5.75 7. desoxycorticosterone 25.94 Inj.: 5µL Conc.: 00µg/mL each component Sample diluent: methanol Column: Allure Biphenyl Cat. #: 966565 Dimensions: 50 x 4.6 mm Pore size: 60Å water:acetonitrile, 60:40 ml/min. Temp.: ambient UV @ 254 nm LC_PH0330 new! 4 Concentration Peak List (µg/ml). hydrocortisone 67 2. prednisone 53 3. cortisone 53 4. betamethasone 73 5. dexamethasone 52 Excellent resolution of isomers! 2 3 5 Inj.: 0µL Sample diluent: methanol Column: Allure Biphenyl Cat.#: 566365 Dimensions: 50 x 4.6 mm Particle size: 3µm Pore size: 60Å Temp.: water:acetonitrile, 70:30 (v/v).5ml/min. ambient UV @ 220nm LC_PH0384

Steroids 93 Endogenous Steroids on Allure Biphenyl Ret. Time Peak List (min.). β-estradiol 3.76 2. testosterone 5.08 3. progesterone 4.05 Inj.: 5µL Conc.: 00µg/mL each component Sample diluent: methanol Column: Allure Biphenyl Cat. #: 966565 Dimensions: 50 x 4.6 mm Pore size: 60Å water:acetonitrile, 40:60 ml/min. Temp.: ambient UV @ 220 nm Steroid Hormones on Allure Biphenyl LC_PH0334 Contraceptive Hormones on Allure Biphenyl Ret. Time Peak List (min.). β-estradiol 3.80 2. ethynyl estradiol 4.45 3. norethindrone 6.5 4. norgestrel 8.4 Inj.: 5µL Conc.: 00µg/mL each component Sample diluent: methanol Column: Allure Biphenyl Cat. #: 966565 Dimensions: 50 x 4.6 mm Pore size: 60Å Temp.: water:acetonitrile, 40:60 ml/min. ambient UV @ 220 nm LC_PH0328 new! HPLC APPLICATIONS PHARMACEUTICAL 2 3 Resolve steroid hormones in a simple, isocratic analysis. Excellent resolution, including isomers! Peak List. estriol 2. 7β-estradiol 3. 7α-estradiol 4. 7α-ethynyl estradiol 5. testosterone 6. estrone 7. norethindrone 4 5 6 7 Inj.: 0µL Conc.: 50µg/mL each component Sample diluent: acetonitrile:methanol, 4: (v/v) Column: Allure Biphenyl Cat.#: 566365 Dimensions: 50 x 4.6 mm Particle size: 3µm Pore size: 60Å LC_BP0385 Temp.: water:acetonitrile, 50:50 (v/v).5ml/min. ambient UV @ 220nm

94 Steroids, Levothyroxine, Basic Drugs Contraceptive Hormones on Allure Basix Hormone Levothyroxine on Ultra PFP HPLC APPLICATIONS PHARMACEUTICAL. uracil (marker) 2. norethindrone 3. medroxyprogesterone Inj.: 0µL Conc.: 00µg/mL Solvent: mobile phase Column: Allure Basix Cat. #: 96565 Dimensions: 50 x 4.6mm Pore size: 60Å water:acetonitrile (40:60, v/v).0ml/min. Flow rate: Temp.: 30 C UV @ 220nm 0 0.5.5 2 2.5 3 3.5 4 min. LC_0083 Basic Drugs on phidelity C8 2 3 2. unknown 2. levothyroxine Inj.: 20µL Conc.:.mg/mL Solvent: water:methanol (70:30) Add 3 drops of concentrated ammonium hydroxide to each 0mL of solvent. Levothyroxine will not dissolve unless the ph is basic. Column: Ultra PFP Cat. #: 976565 Dimensions: 50 x 4.6mm Pore size: 00Å 0mM ammonium formate, ph 2.5: methanol (60:40, v/v) Flow rate:.2ml/min. Temp.: 25 C UV @ 254nm 0 2 4 6 8 0 2 4 6 8 min. LC_0093 new! Retention Time Peak List pka (min.). lidocaine 7.9.904 2. verapamil 8.9 2.20 3. diphenhydramine 9.0 2.39 4. nortriptyline 9.7 3.366 Inj.: 0µL Conc.: 00µg/mL each component Sample diluent: acetonitrile Column: phidelity C8 Cat.#: 9579365 Dimensions: 50 x 4.6 mm Particle size: 3µm Pore size: 200Å Temp.: 0mM ammonium bicarbonate (ph ): acetonitrile: tetrahydrofuran, 35:55:0 (v/v/v).5ml/min. ambient UV @ 254 nm LC_PH0363

Lactulose Concentrate, Xanthines, Sedatives 95 Laxative Lactulose Concentrate by USP 25: Resolution Solution on Pinnacle II Amino Conc. (mg/ml) Ret. Time (min.) RRT Res. Inj. % RSD (n=5). fructose 0.4 4.6 0.44 2. galactose 6.4 6. 0.58 3. epi-lactose unknown 0.0 0.95 4. lactulose 40 0.5.00. 0.3% 5. lactose 4.8 2.7.2 2.9 Inj.: 20µL resolution standard Solvent: acetonitrile:water (50:50) Column: Pinnacle II Amino Cat. #: 927365 Dimensions: 50 x 4.6mm Particle size: 3µm Pore size: 0Å LC_0222 Sedatives Alprazolam and Lorazepam on Allure Basix 0mM sodium phosphate monobasic in water, ph 4.8: acetonitrile (22:78, v/v).3ml/min. Temp.: 40 C refractive index @ 40 C Xanthines on Pinnacle DB C8 2 0 2 3 4 5 6 7 min. Sedative Cyclobenzaprine on Allure Basix 3 Conc. (µg/ml). theobromine 225 2. theophylline 48 3. β-hydroxyethyltheophylline 48 4. caffeine 400 LC_024 4 Inj.: 2µL Solvent: mobile phase Column: Pinnacle DB C8 Cat. #: 944565 Dimensions: 50 x 4.6 mm Pore size: 40Å 20mM KH2PO4, ph 3.0: acetonitrile (90:0 v/v).0 ml/min. Temp.: 27 C UV @ 254nm 2 HPLC APPLICATIONS PHARMACEUTICAL. toluene (marker) 2. lorazepam 3. alprazolam Inj.: 0µL Conc.: 500µg/mL Solvent: water:acetonitrile (7:3, v/v) Column: Allure Basix Cat. #: 96565 Dimensions: 50 x 4.6mm Pore size: 60Å 0mM ammonium formate, ph 3.0:acetonitrile (70:30, v/v) Flow rate: 2.0mL/min. Temp.: 25 C UV @ 230nm 2 3. toluene (marker) 2. cyclobenzaprine HCl Inj.: µl Conc.:.5µg/mL Solvent: 00% water Column: Allure Basix Cat. #: 96565 Dimensions: 50 x 4.6mm Pore size: 60Å 0mM ammonium formate, ph 3.0 acetonitrile (25:75, v/v) Flow rate:.2ml/min. Temp.: 30 C UV @ 230nm 0 2 3 4 5 min. LC_0078 0 2 3 4 5 6 min. LC_0087

96 Admixed Drugs Admixtures: Analgesic Ibuprofen and Decongestant Pseudoephedrine on Allure Basix Admixtures: Analgesic Acetaminophen, Decongestant Pseudoephedrine and Stimulant Caffeine on Allure Basix HPLC APPLICATIONS PHARMACEUTICAL. uracil 2. ibuprofen 3. pseudoephedrine 2 3 LC_0098 Inj.: 5µL Conc.: 200µg/mL Solvent: mobile phase Column: Allure Basix Cat. #: 96565 Dimensions: 50 x 4.6mm Pore size: 60Å 0mM ammonium acetate, ph 6.9: methanol (75:25, v/v).0ml/min. Temp.: 25 C UV @ 20nm 0 2 4 6 8 0 2 4 min. Admixtures: Hydrocodone Bitartrate/Acetaminophen by USP 25 on Ultra C8 Conc. Ret. Time RRT Tailing Resolution Inj. %RSD (µg/ml) (min.) (n=5). acetaminophen 0.76 3.304.0.. 2. hydrocodone bitartrate 0.0076 6.664 2.0. 7.0 0.9 Inj.: 25µL 500mg acetaminophen with 5mg hydrocodone bitartrate (tablet) Solvent: mobile phase Column: Ultra C8 Cat. #: 974575 Dimensions: 250 x 4.6mm Pore size: 00Å Temp.: 27 C 6.8g potassium monobasic with 0.2mL TEA per liter: acetonitrile (85:5, v/v).5ml/min. UV @ 20nm (hydrocodone bitartrate) and 295nm (acetaminophen). 2 3. sample solvent 2. caffeine 3. acetaminophen 4. pseudoephedrine Inj.: 5µL Conc.: 00µg/mL Solvent: mobile phase Column: Allure Basix Cat. #: 96565 Dimensions: 50 x 4.6mm Pore size: 60Å 0mM ammonium acetate, ph 6.9: methanol (75:25, v/v).0ml/min. Temp.: 25 C UV @ 20nm 0 2 4 6 8 0 2 min. LC_0097 Admixtures: Expectorant/Antitussive Guaifenesin and Narcotic Analgesic Codeine on Ultra Phenyl Conc. (µg/ml). codeine 825 2. guaifenesin 375 Inj.: 5µL Solvent: methanol:water (50:50) Column: Ultra Phenyl Cat. #: 905565 Dimensions: 50 x 4.6mm Pore size: 00Å 0mM ammonium formate, ph 2.5: acetonitrile (80:20, v/v) Flow rate:.2ml/min. Temp.: 25 C UV @ 280nm 4 2 0 2 3 4 5 6 min. LC_022 LC_0088

HPLC APPLICATIONS 97 acenaphthene...................... 76 acenaphthylene..................... 76 acetaldehyde.................... 2, 69 acetaminophen.................. 84, 96 acetic acid......................... 80 acetone........................ 2, 69 acifluorfen......................... 72 acrolein........................ 2, 69 adenine........................... 66 adenosine......................... 66 ADP.............................. 66 AHD (-aminohydantoin hydrochloride).... 83 albuterol.......................... 9 aldicarb........................... 73 aldicarb sulfone.................... 73 aldicarb sulfoxide................... 73 allicin............................. 77 alprazolam......................... 95 2-amino-4,6-dinitrotoluene......... 7, 70 4-amino-2,6-dinitrotoluene......... 7, 70 amitriptyline....................... 89 amoxicillin......................... 88 AMOZ (3-amino-5-morpholinomethyl- 2-oxazolidinone).................. 83 d5-amoz (3-amino-5-morpholinomethyl- 2-oxazolidinone).................. 83 AMP.............................. 66 angiotensin I....................... 68 angiotensin II, III................... 68 anthracene........................ 76 AOZ (3-amino-2-oxazolidinone)......... 83 d4-aoz (3-amino-2-oxazolidinone)...... 83 apomyoglobin...................... 68 ascorbic acid....................... 78 atenolol........................... 86 ATP.............................. 66 atrazine........................... 74 beclomethasone.................... 88 bentazon.......................... 72 benzaldehyde................... 2, 69 benzene............................ 9 benzo(a)anthracene................. 76 benzo(b)fluoranthene................ 76 benzo(k)fluoranthene................ 76 benzo(ghi)perylene.................. 76 benzo(a)pyrene..................... 76 benzoic acid.................... 79, 84 betamethasone..................... 92 BHA.............................. 79 2-BHA............................ 79 3-BHA............................ 79 BHT.............................. 79 biphenyl............................ 9 butyl paraben...................... 78 butyraldehyde................... 2, 69 caffeine........................ 95, 96 caftaric acid....................... 77 capsaicin.......................... 79 carbaryl........................... 73 carbazole.......................... 7 carbofuran......................... 73 CDP.............................. 66 cephalexin......................... 88 cephaloridine....................... 89 cephradine........................ 88 chloramben........................ 72 chlorogenic acid.................... 77 chlortetracycline.................... 90 chlortoluron..................... 73, 74 chrysene.......................... 76 cichoric acid....................... 77 cimetidine......................... 9 citric acid....................... 80, 8 CMP.............................. 66 COC.............................. 67 cocaine........................... 67 codeine........................ 84, 96 codeine phosphate............... 84, 85 corticosterone................... 9, 92 cortisone....................... 9, 92 cortisone acetate................ 9, 92 crotonaldehyde.................. 2, 69 cyanazine...................... 73, 74 cyclobenzaprine HCl................. 95 cytidine........................... 66 cytochrome c....................... 68 cytosine........................ 66, 67 2,4-D.......................... 7, 72 2,4-DB............................ 72 deschlorobeclomethasone............ 88 desimipramine..................... 89 desisopropylatrazine................. 74 desoxycorticosterone................ 92 dexamethasone..................... 92 dibenzo(a,h)anthracene.............. 76 dicamba........................... 72,4-dichlorobenzene.............. 7, 72 3,5-dichlorobenzoic acid.............. 72 2,4-dichlorophenylacetic acid.......... 72 dichlorprop........................ 72 diflubenzuron...................... 7 digitoxin........................... 86 digoxigenin........................ 86 digoxin............................ 86 dihydrocapsaicin.................... 79 2,4-dihydroxybenzoic acid............. 84 2,5-dihydroxybenzoic acid............. 84 3,5-dihydroxybenzoic acid............. 84 diltiazem.......................... 85,7-dimethylxanthine................. 67,2-dinitrobenzene............... 7, 70,3-dinitrobenzene............... 7, 70 3,4-dinitrobenzene.................. 70 2,4-dinitrotoluene................ 7, 70 2,6-dinitrotoluene................ 7, 70 3,4-dinitrotoluene................ 7, 70 dinoseb........................... 72 diphenhydramine................... 94 diquat......................... 28, 75 diuron...................... 7, 73, 74 dopamine.......................... 68 doxepin........................... 89 doxycycline........................ 90 ecgonine methyl ester............... 67 echinacoside....................... 77 EME.............................. 67 enalapril.......................... 86 epi-lactose......................... 95 epinephrine........................ 68 7α-estradiol...................... 93 7β-estradiol...................... 93 β-estradiol........................ 93 estriol............................ 93 estrone........................... 93 ethofumesate................... 73, 74 ethyl paraben...................... 78 ethyl vanillin....................... 82 ethyluracil......................... 67 ethynyl estradiol.................... 93 7α-ethynyl estradiol................ 93 fluometuron........................ 7 fluoranthene....................... 76 fluorene........................... 76 fluoxetine.......................... 90 flusilazole...................... 73, 74 fluvoxamine maleate................. 90 formaldehyde................... 2, 69 fructose........................ 8, 95 fumaric acid....................... 80 furosemide........................ 86 galactose.......................... 95 gitoxigenin......................... 86 gitoxin............................ 86 glucose........................... 8 glyburide.......................... 9 guaifenesin........................ 96 guanine........................... 66 guanosine......................... 66 hexanaldehyde.................. 2, 69 HMX........................... 7, 70 holo-transferrin..................... 68 hydrochlorothiazide................. 87 hydrocodone bitartrate......... 84, 85, 96 hydrocortisone..................... 92 3-hydroxybenzoic acid................ 84 4-hydroxybenzoic acid................ 84 3-hydroxycarbofuran................. 73 hydroxycodone..................... 84 β-hydroxyethyltheophylline............ 95 hypoxanthine....................... 67 ibuprofen.......................... 96 imipramine........................ 89 indeno(,2,3-cd)pyrene............... 76 isocitric acid....................... 80 isoproturon..................... 73, 74 lactic acid......................... 80 lactose......................... 8, 95 lactulose.......................... 95 leucine enkephalin.................. 68 levodopa.......................... 68 levothyroxine....................... 94 lidocaine.......................... 94 linuron...................... 7, 73, 74 lorazepam......................... 95 maleate........................ 86, 87 malic acid...................... 80, 8 malonic acid....................... 80 maltose........................... 8 MCPA............................. 72 MCPP............................. 72 medroxyprogesterone................ 94 MEK.............................. 69 menadione......................... 78 meth enkephalin.................... 68 methabenzthiazuron.............. 73, 74 methacrolein.................... 2, 69 methiocarb........................ 73 methomyl.......................... 73 methyl ethyl ketone............... 2, 69 methyl paraben..................... 78 5-methylcytosine.................... 67 6-methylpurine..................... 67 metoprolol......................... 87 monuron.......................... 7 morphine sulfate................. 84, 85 N,N'-bis-(chlorobenzyl)urea........... 7 naphthalene..................... 9, 76 naphthoate........................ 89 neburon........................ 73, 74 nicardipine......................... 85 nifedipine......................... 85 nitrobenzene.................... 7, 70 4-nitrophenol....................... 72 2-nitrotoluene................... 7, 70 3-nitrotoluene................... 7, 70 4-nitrotoluene................... 7, 70 nordihydrocapsaicin................. 79 nordoxepin........................ 89 norepinephrine..................... 68 norethindrone................... 93, 94 norgestrel......................... 93 nortriptyline..................... 89, 94 oxamyl............................ 73 oxycodone......................... 84 oxycodone HCl................... 84, 85 oxytetracycline..................... 90 paraquat....................... 28, 75 pendimethalin...................... 73 penicillin V......................... 88 pentachlorophenol.................. 72 phenanthrene...................... 76 phenethyl glucosinolate.............. 77 phenol............................ 85 phenylalanine...................... 67 phylloquinone...................... 78 picloram.......................... 72 piperine........................... 79 prednisone........................ 92 progesterone....................... 93 propanil........................... 7 propionaldehyde................. 2, 69 propoxur.......................... 73 propyl gallate...................... 79 propyl paraben..................... 78 propyluracil........................ 67 protriptyline........................ 89 pseudoephedrine................... 96 purine............................ 67 pyrene............................ 76 pyruvic acid........................ 80 quinic acid...................... 80, 8 RDX........................... 7, 70 all-trans-retinol..................... 78 ribonuclease A..................... 68 salicylic acid....................... 84 SC (semicarbazide).................. 83 secbumeton..................... 73, 74 sertraline HCl...................... 90 siduron........................... 7 Silvex.......................... 7, 72 simazine.......................... 74 sorbic acid......................... 79 succinic acid....................... 80 sucrose........................... 8 Sudan I........................... 82 Sudan II.......................... 82 Sudan III.......................... 82 Sudan IV.......................... 82 sulfamethoxazole................... 89 2,4,5-T............................ 72 tartaric acid........................ 80 TBHQ............................. 79 TDP.............................. 66 tebuthiuron........................ 7 terbumeton........................ 74 terbuthylazine...................... 74 terbuthylazine desethyl............... 74 testosterone....................... 93 tetracycline........................ 90 Tetryl.......................... 7, 70 theobromine.................... 67, 95 theophylline..................... 67, 95 thiamin........................... 78 thidiazuron........................ 7 thymidine.......................... 66 thymine........................ 66, 67 timolol............................ 87 TMP.............................. 66 α-tocopherol.................... 77, 78 α-tocopherol acetate................ 78 β-tocopherol....................... 77 δ-tocopherol....................... 77 γ-tocopherol....................... 77 m-tolualdehyde.................. 2, 69 toluene........................... 95 2,4,5-TP (Silvex).................... 72 triamcinolone...................... 92 triamterene........................ 87 trimethoprim....................... 89,3,5-trinitrobenzene............. 7, 70 2,4,6-trinitrotoluene.............. 7, 70 tryptophan......................... 67 TTP.............................. 66 tyrosine........................ 67, 68 UDP.............................. 66 UMP.............................. 66 uracil............ 9, 66, 67, 86, 90, 92, 94 uric acid........................... 67 uridine............................ 66 UTP.............................. 66 val-4-angiotensin III................. 68 val-tyr............................ 68 val-tyr-val......................... 68 valeraldehyde................... 2, 69 vanillin......................... 8, 82 verapamil...................... 85, 94 verapamil HCl...................... 87 vitamin D3......................... 78 xanthine........................... 67 Compound Index Restek Trademarks: Allure, phidelity, Pinnacle, Restek logo, Sidewinder, Trident. 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