D ATA S H E E T EVAPORATIVE LIGHT SCATTERING DETECTOR ELSD for Routine HPLC and GPC Analysis Evaporative light scattering detectors are ideal for detecting analytes with no UV chromophore as they do not rely on the optical properties of a compound. The Evaporative Light Scattering Detector is the latest high performance ELS detector from Varian Polymer Laboratories, with almost 20 years experience in the design and manufacture of evaporative light scattering detectors. The Evaporative Light Scattering Detector is the smallest and most flexible ELSD available. Patented gas flow technology gives the 380-LC unrivalled detection capability at ambient temperatures and uniform response across a gradient. This precision instrument operates up to 20 C, with a heated nebulizer that makes it a perfect match for a wide range of applications and LC techniques, including GPC. To detect everything you inject with high sensitivity, the is the ELSD of choice for all high temperature applications. Key Benefits High sensitivity provides superb responses for all compounds, down to low nanogram levels. Unrivalled response to semi-volatile compounds at ambient temperature from patented gas flow technology. Operation up to 20 oc gives improved evaporation of difficult solvents and better response to non-volatile compounds. Low dispersion and high-speed data output rates are the perfect match for Fast LC applications. Superb reproducibility below 2 % gives reliable and accurate results. Full DMSO transparency ensures that responses from early eluting compounds are not hidden. Multi-vendor software control and data acquisition using Varian s Galaxie chromatography data system, and other vendors interfaces, eliminates the need for an analog to digital converter. Fully compatible with the Varian 920-LC analytical HPLC system for enhanced chromatographic performance. Complementary to LC/MS. The smallest ELSD footprint frees up bench space for a less cluttered and safer working environment. Simple set up means that you can be up and running in less than five minutes, straight out of the box.
The is an advanced evaporative light scattering detector from Polymer Laboratories, now a part of Varian, Inc. The 380-LC is ideal for the detection of non-volatile compounds, where temperatures of 00 o C or above are required. The instrument benefits from fast data output rates and extremely low dispersion for fast LC, and delivers a universal response down to the low-nanogram range for truly representative analysis. Reproducibility is less than 2 % for improved consistency of results. Control and digital data collection come as standard for multi-vendor platforms and so there is no need for an analog to digital converter. On-the-fly adjustment of light source intensity can save time during a run. All this in the smallest footprint around. Being complementary to LC/MS, and offering unrivalled flexibility and sensitivity, the is the ELSD of choice for demanding, high temperature applications. Varian s Family of Evaporative Light Scattering Detectors ELS detection is the powerful alternative to RI and UV for all compounds; just inject it and detect it, right down to low nanogram levels. ELSDs provide a universal response independent of the optical properties of the analyte and so there is no loss of important information. You can use Varian s ELSDs for a very wide range of analytical techniques, including HPLC (analytical and preparative), LC/MS, SFC, high throughput screening, GPC/SEC, TREF and GPEC. The range of application areas is equally broad, encompassing pharmaceuticals, nutraceuticals, combinatorial libraries, carbohydrates, lipids, phopspholipids, triglycerides, fatty acids, amino acids, polymers and surfactants. Choose the right Varian ELSD for your needs. HPLC analysis: Non-volatile compounds Semi-volatile compounds Highly volatile compounds Ambient GPC analysis High temperature GPC analysis Varian 385-LC Varian 380-LC PL-ELS 000 yes yes yes yes yes x yes x x yes yes yes x x yes Instrument Qualification - IQ, OQ All Varian Polymer Laboratories ELSD detectors are rigorously tested to a high specification before they are shipped, and detailed IQ, OQ documentation is included with every unit. In addition, our detectors can be incorporated into any HPLC system, and a complete Qualification Workbook is provided to help you. Uniform Mass Response Varian ELSDs are not dependent on a compound s optical properties and so the ELSD provides a more uniform response than UV-VIS, making it the ideal detector for purity analysis or where calibration standards are not available. Column: Polaris C8 5 µm, 50 x 4.6 mm Eluent: Water:Acetonitrile : Flow Rate:.0 ml/min Injection Volume: 0 µl Detection: (neb=30 o C, evap=30 o C, gas=.4 SLM) For non-volatile compounds, where temperatures of 00 C or above are required, the is the instrument of choice. The Varian 385-LC delivers sub-ambient evaporation down to 0 C, providing maximum sensitivity for compounds with significant volatility below 30 C. prednisone dexamethasone UV @ 280 nm The PL-ELS 000 is designed for routine GPC, including high boiling point solvents. For further information on Varian s other ELSDs, see our datasheets Varian 385-LC and PL-ELS 000. 0 2 min 3 4 5 Figure. The Varian ELSD delivers a more uniform mass response than UV from a : mixture. Page 2
Varian ELSDs - Better by Design Evaporative light scattering detection involves a three stage process.. Nebulization utilizing an inert gas stream to form a plume of uniformly sized droplets. 2. Evaporation of the eluent generating a plume of nonvolatile solute particles. 3. Optical detection where the intensity of scattered light is proportional to the mass of solute passing through the optical chamber. Nebulization Efficient nebulization using low gas flow rates is a feature of Varian ELS detectors. Independent nebulizer temperature control and digital gas flow control provide excellent stability and reproducibility. Baseline noise is minimized by the removal of any poorly nebulized eluent via a drain port. Separation of Non-volatile Compounds The is ideal for non-volatile compounds with a weak or no chromophore, such as surfactants, sugars, polymers and lipids, where evaporation temperatures of >80 o C are advantageous. Column: Phenyl hexyl 5 µm, 50 x 4.6 mm Eluent A: 0.5 g/l Ammonium acetate in Water Eluent B: Methanol Gradient: 70-00 % B in 4 min Temperature: 35 C Flow Rate:.0 ml/min Detection: (neb=35 C, evap=60 C, gas=. SLM) Peak Identification. Erucamide 2. Tinuvin 326 3. Irgafos 68 4. Irganox 2 3 4 Evaporation The nebulized stream passes through an independently temperature-controlled evaporator tube where solvent is removed at high temperature, leaving the less volatile solute particles behind. The features patented gas flow control technology with a short evaporator tube that gives an extremely low swept volume for minimal peak dispersion. This provides maximum resolution from the separation, especially important for work with small columns. 0.3 2.6 min 3.9 5.2 6.9 Figure 2. Excellent baseline stability of four polymer additives. Column: C8 5 µm, 250 x 4 mm Eluent A: Acetonitrile Eluent B: Dichloromethane Gradient: 25-45 % B in 45 min, 45-90 % B in 5 min, hold Flow Rate: 5 µl Detection: (neb=60 C, evap=50 C, gas=.6 SLM) diglycerides gas inlet detector eluent inlet triglycerides liquid waste light source Optical detection The solute particles are detected as they pass through the optical chamber. The high power LED and advanced design of the electronics delivers maximum sensitivity. monoglycerides fatty acids UK Patent 0304253.8, US Patent 6/0238744 0 min 30 Figure 3. Separation of a sample of sesame oil. Page 3
Separation of Semi-Volatile Compounds The is also ideal for separating compounds with different degrees of volatility, as shown in Figure 4. With the nebulizer and evaporator running at the same temperature, 50 o C, non-volatile indapamide gives a large response but the other more volatile components give very poor peak response due to loss through evaporation. As the temperatures are reduced to 35 o C, and even more so at ambient, the reveals much improved recovery data of the semi-volatile components, with no deterioration in baseline stability. Peak Identification. Acetanilide 2. Indapamide 3. Ibuprofen 4. Dibutylphthalate Sample: 2 mg/ml in DMSO 50 o C Column: C8, 5 µm, 50 x 4.6 mm Eluent A: 0. % TFA in Water Eluent B: 0. % TFA in Acetonitrile Gradient: 60-90 % in 5 min Flow Rate:.0 ml/min Injection Volume: 20 µl Sample Concentration: mg/ml Detection: Varian ELSD (gas=.6 SLM) DMSO Transparency for High Throughput Screening The vast majority of combinatorial libraries screened by high throughput methods are stored in dimethylsulfoxide (DMSO). UV and mass spectrometry will miss potential drug compounds that have no UV chromophore and so ELSD is commonly used. However, at the low evaporator temperatures required to detect volatile components, the presence of DMSO in the sample can mask the response of early eluting compounds. In the Varian 380- LC, the addition of a carefully controlled stream of gas to the evaporation step enables complete removal of the DMSO to take place without increasing the operating temperature. Column: 5 µm, 50 x 4.6 mm Eluent A: 0. % HFBA in Water Eluent B: 0. % HFBA in Acetonitrile Gradient: 5-30 % B in 0 min, 30-80 % B in 5 min Flow Rate:.0 ml/min Injection Volume: 20 µl Detector: (neb=25 C, evap=25 C, gas as shown) 35 0 C DMSO 2 3 4 ambient gas=.2 SLM 0 min 9 Figure 4. The ability of the to operate at ambient improves data quality. gas=.8 SLM 0 min 5 Figure 5. Increasing gas flow on the provides DMSO transparency. Page 4
Superb RSD Excellent reproducibility below 2 % gives reliable and accurate results - you can have complete confidence in your data. Column: Pursuit C8 5 µm, 50 x 4.6 mm Eluent: Water:Acetonitrile 40:60 Flow Rate:.0 ml/min Injection Volume: 0 µl Detection: (neb=40 C, evap=40 C, gas=.4 SLM) Improved Sensitivity over an RI Detector The ELSD has better baseline stability and sensitivity than an RI detector, making it an extremely suitable choice for carbohydrate analysis. Column: PL Hi-Plex Ca, 250 x 4 mm Eluent: Water Flow Rate: 0.6 ml/min Temperature: 85 o C Injection Volume: 0 µl Detection: (neb=30 C, evap=30 C, gas=.6 SLM) RI Peak Identification. Melezitose 2. Maltose 3. Glucose 4. Mannose 5. Fructose 6. Ribitol (adonitol) 2 3 4 5 6 0 2 3 4 5 min Figure 6. Fifty caffeine injections produce an RSD of.27 %, demonstrating the excellent reproducibility of of the. Sophisticated Galaxie Software for Integrated Control of the System, Data Analysis and Reporting Control and data acquisition using Varian s Galaxie chromatography data system, with options for other multi-vendor control, provides maximum flexibility for the with any HPLC or GPC system. 0 min 23 Figure 7. The ELSD delivers more stable baselines and much improved sensitivity compared to an RI detector. Page 5
Specification Light source Detector LED 480 nm (Class LED Product) Photomultiplier tube digital signal processing Temperature range Evaporator OFF, 30-20 C ( C increments) Nebulizer OFF, 30-90 C ( C increments) Gas requirements Flow rate 0.9 SLM to 3.25 SLM @ 25 C with integrated controlled gas shutoff valve Eluent flow rate Analogue output Digital output Communication Instrument operation PC operation (software) Power requirements Detector status Pressure operating range 0.2-5 ml/min 0 - V FSD 24 bit digital data, 0 or 40 Hz Serial (RS232) Remote start Input Remote A/Z Contact closure 60 00 psi (4-6.7 bar) TTL Graphical vacuum fluorescent display with keypad Real-time control via ELSD Dimension Software Ten predefined methods Galaxie Chromatography Software, standalone control software utilities, Chromeleon Software, ChemStation Control Software, EZChrom Elite Control Software (released later in 2008) 90/20V AC or 220/250V AC 50/60 Hz 2A max Standby, run Size Unpackaged 200 x 450 x 45 mm (w x d x h) Weight Unpackaged 3 kg Safety features Gas shut off valve, vapor and leak detection Ordering Information Product Description Part Number (0 V) PL0890-00 (240 V) PL0890-0240 Varian ELSD Dimension Software PL0890-0375 Varian ELSD Driver for ChemStation PL0890-0380 Galaxie LC Driver Software 850244000 Varian, Inc. www.varianinc.com North America: 800.926.3000, 925.939.2400 Europe The Netherlands: 3.8.67.000 Asia Pacific Australia: 63.9560.733 Latin America Brazil: 55..3238.0400 Other sales offices and dealers throughout the world check our Web site GC LC MS GPC/SEC AA ICP ICP-MS UV-Vis-NIR FT-IR Fluorescence Dissolution NMR MRI FTMS Consumables Data Systems Polymer Laboratories (PL), the PL Logo, Galaxie, Polaris, Pursuit, Chromeleon, EZChrom Elite, Varian and the Varian Logo are trademarks or registered trademarks of Varian, Inc. in the U.S. and other countries. SI-287 03/08 Printed in UK 2008 Varian, Inc.