Warning: Some material in this presentation and related videos may be too graphic for some people. T. Trimpe 2009

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Warning: Some material in this presentation and related videos may be too graphic for some people. T. Trimpe 2009 http://sciencespot.net/

What do they do? Forensic entomologists apply their knowledge of entomology to provide information for criminal investigations. A forensic entomologist s job may include: Take picture of insects on the body at the crime scene before removing insects from corps. Identification of insects at various stages of their life cycle, such as eggs, larva, and adults. Collection and preservation of insects as evidence. Determining an estimate for the postmortem interval or PMI (the time between death and the discovery of the body) using factors such as insect evidence, weather conditions, location and condition of the body, etc. Testifying in court to explain insect-related evidence found at a crime scene. Did you know? Maggots can be used to test a corpse for the presence of poisons or drugs. Some drugs can speed up or slow down the insect s development. Cool Jobs: Forensic Entomology Discovery Video

Insects as Evidence Forensic entomologists use their knowledge of insects and their life cycles and behaviors to give them clues about a crime. Most insects used in investigations are in two major orders: 1 Flies (Diptera) and 2 Beetles (Coleoptera) Blow Fly Carrion Beetle Species succession may also provide clues for investigators. Some species may to feed on a fresh corpse, while another species may prefer to feed on one that has been dead for two weeks. Investigators will also find other insect species that prey on the insects feeding on the corpse. Images: Top Right - http://www.insectinvestigations.com/aboutfe.htm, Chart - http://www.clt.uwa.edu.au/ data/page/112507/fse07_forensic_entomology.pdf

Arthropods arthropods constitute over 90% of the animal kingdom and are classified in the phylum Arthropoda. They are distinguished from other animals by: an exoskeleton (a skeleton on the outside of the body) body divided into distinct parts jointed legs and appendages bilateral symmetry (both sides of the body are the same)

Taxonomic identification of the insects found on corpses is essential to the reconstruction of events surrounding criminal cases involving death. Systems of classification of biological organisms are used to facilitate their identification.

Weather data is also an important tool in analyzing insect evidence from a corpse. Investigators will make note of the temperature of the air, ground surface, the interface area between the body and the ground, and the soil under the body as well as the temperature inside any maggot masses. They will also collect weather data related to daily temperature (highs/lows) and precipitation for a period of time before the body was discovered to the time the insect evidence was collected. Other factors that might affect their PMI estimates: 1. Was the body enclosed in an area or wrapped in a material that would have prevented flies from finding the corpse and laying eggs? 2. Were other insect species present that may have affected the development of the collected species? 3. Were there drugs or other poisons in or on the body that might have affected the larvae s development? Did you know The Body Farm in Knoxville, Tennessee is a university research facility to investigate human decomposition under various conditions in order to understand the factors which affect its rate. Click the image to view a video about the Body Farm!

Maggot Mass Effect Maggot mass effect is the forming of maggots on a decomposed animal or human body. Temperature plays a large part on how fast a maggot mass forms and grows. Maggots grow slower in cooler temperatures. Metabolic activity can cause temperatures within the maggot mass to rise by 5-20 C compared to the ambient and ground temperatures.

Blow Fly Metamorphosis Blow flies are attracted to dead bodies and often arrive within minutes of the death of an animal. They have a complete life cycle that consists of egg, larva, pupa, and adult stages. 1st Adult flies lay eggs on the carcass especially at wound areas or around the openings in the body such as the nose, eyes, ears, anus, etc. 2nd Eggs hatch into larva (maggots) in 12-24 hours. Pupa Adult Eggs 3rd Larvae continue to grow and molt (shed their exoskeletons) as they pass through the various instar stages. 1st Instar - 5 mm long after 1.8 days 2nd Instar - 10 mm long after 2.5 days 3rd Instar 14-16 mm long after 4-5 days 4th The larvae (17 mm) develop into pupa after burrowing in surrounding soil. 5th Adult flies emerge from pupa cases after 6-8 days. 3 rd Instar Larva 2 nd Instar Larva 1 st Instar Larva It takes approximately 14-16 days from egg to adult depending on the temperatures and humidity levels at the location of the body. Image: http://www.umext.maine.edu/images/flylife.jpg Information: http://www.kathyreichs.com/entomology.htm and http://www.forensicentomologist.org/

Examples of Diptera (Flies) Early Stage Decomposition Blow & Greenbottle Flies (Calliphoridae) Metallic thorax and abdomen Flesh Fly (Sarcophagidae) Striped thorax Life Cycle of a Calliphoridae Fly Late Stage Decomposition House Fly (Muscidae) Cheese Skipper (Piophilidae) Informational Source: http://naturalsciences.org/files/documents/csi_tg_overview.doc Images: Top Left - http://www.scienceinschool.org/repository/images/issue2forensic3_large.jpg, Middle-Left: http://forensicfact.files.wordpress.com/2008/04/blowfly053.jpg, Top Right - http://users.usachoice.net/~swb/forensics/p1.jpg, Bottom - http://www.deathonline.net/decomposition/corpse_fauna/flies/index.htm

Examples of Coleoptera (Beetles) Early Stage Decomposition Early to Late Stage Decomposition Carrion Beetles (Silphidae) Adults & larvae feed on fly larvae Rove Beetles (Staphylinidae) Predator of fly eggs Clown Beetles (Histeridae) Predator of fly eggs Late Stage Decomposition Ham & Checkered Beetles (Cleridae) Predator of flies & beetles; also feed on dead tissue Skin Beetles (Dermestidae) Feed on dried skin & tissues Hide Beetles (Scarabidae) Usually the last to arrive Informational Source: http://naturalsciences.org/files/documents/csi_tg_overview.doc Images: http://www.cals.ncsu.edu/course/ent425/library/spotid/coleoptera/coleoptera.html & http://www.forensicflies.com/beetles.htm

Let s give it a try Click the image above or click here to visit the website at http://www.pbs.org/wnet/nature/episodes/crime-scene-creatures/interactive-determine-the-time-of-death/4390/