YMC-Triart Prep Robust and ph stable hybrid silica process material The Selectivity Company Robust Improved particle properties result in better chromatographic performance Flexible Chemical stability ph 1-12 Durable against alkaline CIP Economic Purify more endproduct on less stationary phase www.ymc.de
2 YMC-Triart Prep Particle Technology... page 3 Physicochemical Characteristics... page 4-5 Mechanical Stability... page 6 Comparing YMC-Triart Prep C18-S to competitor material... page 7 Cleaning-in-place (CIP)... page 8-9 Applications... page 10 Ordering Information... page 11
3 YMC-Triart Prep With YMC-Triart Prep, a ph stable preparative grade HPLC stationary phase gets available. It is produced in lot sizes of up to several hundred kg. This innovative multi-layer hybrid material sets a new benchmark for RP HPLC phases. It is well suited for production scale applications, as it is available up to multi-ton scale. YMC-Triart Prep is chemically stable up to ph 12.0. This provides more flexibility for method development and allows for more efficient cleaning-in-place (CIP) procedures. From real life process development work YMC-Triart Prep has shown to outperform traditional silicabased materials 2- to 4-fold in terms of durability. Longer column lifetimes lead to more kilogram of product produced per kilogram of stationary phase. The results are: improved production procedures and reduced overall costs. Particle technology YMC-Triart Prep is a multilayered material prepared from a well-controlled particle formation process which is adapted from micro-reactor technology. The recently developed production step results in truly narrow particle and pore size distributions! With YMC-Triart Prep otherwise, challenging ph and high temperature conditions are no longer a limitation even to the day-to-day work in laboratories or production. Most importantly, due to its unique particle composition, a balanced hydrophobicity and silanol activity are achieved which makes YMC-Triart Prep a First Choice material in method development! YMC-Triart Prep hybrid structure inorganic siloxane layer organic siloxane layer
4 Specification Base material YMC-Triart Prep C18-S YMC-Triart Prep C8-S inorganic/organic multi-layered hybrid Particle size [µm] 10,15, 20 10, 15, 20 Pore size [nm] 12 20 Specific surface area [m 2 /g] 360 220 Bonding polymeric C18 polymeric C8 End-capping yes yes ph range 1.0 ~ 12.0 1.0 ~ 12.0 Homogeneous and uniform particles* YMC-Triart (12 nm, 5 µm) YMC-Triart Prep (20 nm, 15 µm) X-Bridge HILIC ( TM of Waters Corp.) (13.5 nm, 5 µm) YMC-Triart Prep is produced using micro-reactor technology in the granulation process. The results are spherical particles with a highly uniform particle and pore size distribution. This leads to a reduction of the back-pressure and to more reproducibility in surface modification. * by courtesy of YMC Co., Ltd.
5 Excellent ph stability Acidic condition (ph 1, 70 C) Alkaline condition (ph 11.5, 50 C) (%) of initial retention 100 80 60 40 20 0 The columns are stored in acetonitrile/water/tfa (10/90/1) 70 C, and the performance tested every 20 hours. 0 20 40 60 Time (hr) YMC-Triart Prep C18-S, 15 µm Kromasil C18, 16 µm (Kromasil) ODS-AP, 15 µm (Daiso) Chromatorex C18, 15 µm (Fuji) 80 100 (%) of initial theoretical plate 100 80 60 40 20 0 Chromatorex C18, 15 µm (Fuji) ODS-AP, 15 µm (Daiso) Kromasil C18, 16 µm, Kromasil YMC-Triart Prep C18-S, 15 µm 0 100 200 300 Time (hr) Column: 250 x 6.0 mm ID Eluent: acetonitrile / water (60/40) Flow rate: 1.7 ml/min Temperature: 37 C Detection: UV at 254 nm Sample: butyl benzoate Column: 150 x 4.6 mm ID Eluent: 50 mm TEA in methanol / 50 mm TEA in water (ph 11.5) (20/80, v/v) Flow rate: 1.0 ml/min Detection: UV at 254 nm Sample: caffeine At high ph YMC-Triart Prep C18-S shows lifetimes of up to 4 times longer compared to conventional silica materials. This enables new separations at high ph which are not possible with silica material. Furthermore, the material can endure more CIP-cycles than conventional phases used in industrial processes (see page 8). Advantages of YMC-Triart Prep YMC-Triart Prep ph-stability 1-12 Chemical and mechanical stability Uniform particle size Narrow pore size distribute Your benefit Flexibility Method development Durable against CIP-conditions Cost savings High production throughput Improved packing = improved chromatographic performance High loadability Application Data by courtesy of YMC Co., Ltd.
6 Mechanical stability test Test procedure 1. Column packing Column: DAU-50-1000 Bed length: 100 x 50 mm ID Pressure: 6.5 MPa 2. Pressure measurement Eluent: methanol / water (85/15) Flow rate: 50 ml/min Temperature: ambient 3. Unpacking Psi/m 2000 Mechanical stability (dynamic) 1500 1000 500 0 1 5 10 Packing number YMC-Triart Prep (10 µm, 20 nm) (Hybrid silica) YMC HG-series (10 µm, 20 nm) (High grade silica) Traditional Silica (10 µm, 20 nm) Shearing and crushing of silica particles leads to the formation of fines, which results in an increased backpressure. By using mechanically stable, spherical particles the formation of fines can be reduced. Here, high mechanical stability of YMC-Triart Prep is demonstrated by means of repeated packing of a DAC column. Even after more than 10 repacking cycles for the same material the pressure does not increase. The absence of fines is proven by a constant backpressure. This makes YMC-Triart Prep a valuable asset for method development and especially for process optimisation!
7 YMC Triart Prep C18-S vs. competitor material (client data*) Client data confirms that YMC-Triart Prep C18-S shows retention for hydrophobic compounds comparable to conventional silica materials. At the same time polar molecules are exceptionally well separated. * Client data shows non-optimized scouting runs performed on the respective C18 material packed into 250 x 4.6 mm ID columns. Narrow pore distribution 4500 d Vp/ d logd 4000 3500 3000 2500 2000 1500 1000 500 YMC-Triart Prep Conventional silica-based particle X-Bridge HILIC 0 0 5 10 15 20 Pore size (nm) 25 30 35 A narrow pore size distribution of a stationary phase beneficially affects peak width and sample loading in liquid chromatography. Where micropores account for an artificially high surface area of a stationary phase but are mostly inaccessible to target molecules, mesopores decreases the column efficiency due to a steric hindrance effect, which significantly slows mass transfer. YMC-Triart Prep C18-S exhibits a narrower pore size distribution with fewer micro- and mesopores. This results in improved peak shapes and higher sample loading in your preparative processes. Application Data by courtesy of YMC Co., Ltd.
8 Cleaning-in-place (CIP) Test procedure Sample injection (insulin in human serum) injected 20 times Regeneration with alkaline solution Wash with alkaline solution 0.1 M NaOH / acetonitrile (50/50) 10 CV Neutralisation and wash with organic solvent 1. acetonitrile / water (20/80) 2. acetonitrile / water (90/10) CIP performance of YMC-Triart Prep C8-S Effect of CIP with alkaline solution Durability under repeated CIP Column: YMC-Triart Prep C8-S (20 nm, 10 µm) 250 x 4.6 mm ID Eluent: A) water / TFA (100/0.1) B) acetonitrile / TFA (100/0.1) 28-35% B (0-15 min) Flow rate: 1.0 ml/min Temperature: 25 C Detection: UV at 220 nm Injection: 30 µl Sample: 10 mg/ml insulin bovine + human serum (2:1) Application Data by courtesy of YMC Co., Ltd.
9 CIP performance of YMC-Triart Prep C8-S YMC-Triart Prep C8-S (10 µm, 20 nm) Silica based C8 material (10 µm, 20 nm) Column: 50 x 4.6 mm ID Eluent: A) water / TFA (100/0.1) B) acetonitrile / TFA (100/0.1) 29-36% B (0-2 min), 36% B (2-3 min), 29% B (3-6 min) Flow rate: 1.0 ml/min Temperature: 25 C Detection: UV at 220 nm Injection: 6 µl Sample: 10 mg/ml insulin bovine + human serum (2:1) Column lifetime extended by factor > 2 In production repeated injections of a solution of insulin in human plasma/serum are carried out. Consequently, absorption of protein and other impurities to the surface of the packing material reduces the retention capacity of the column. Methodology: At this point a wash step with an alkaline solution (e.g. 0.1 M NaOH) removed the impurities and restores the full capacity of the column. Problem: Silica materials are unsuitable for alkaline wash conditions, because of their poor durability at high ph. Solution: Hybrid silica based YMC-Triart Prep has an excellent durability at high ph. It is amenable to alkaline wash conditions and thus enables longer column lifetime. This in turn reduces production costs: Lower consumption of packing material and fewer downtimes due to column repacking. An extension of column lifetime by a factor of > two has been achieved! Application Data by courtesy of YMC Co., Ltd.
10 Structurally similar peptides at ph > 7 Easy separation ph-shift Column: YMC-Pack Polymer C18, 250 x 4.6 mm ID Eluent: acetonitrile / water / TFA (18/82/0.05) (ph 2.2) Flow rate: 0.5 ml/min Detection: UV at 220 nm Injection: 10 µl Sample: 1. Angiotensin III 2. Angiotensin II 3. Angiotensin I Column: YMC-Triart Prep C8-S (20 nm, 10 µm) 250 x 4.6 mm ID Eluent: A) acetonitrile / 10 mm NH 4 H 2 PO 4 + NH 4 OH (50/50) (ph 2.2) B) 10 mm NH 4 H 2 PO 4 + NH 4 OH (ph 7) Gradient: 35-65% A in 25 min Flow rate: 1 ml/min Detection: UV at 215 nm Injection: 10 µl Sample: 1. Angiotensin II 2. Angiotensin III 3. Angiotensin I Methods developed on analytical YMC-Triart material are readily transferred to preparative conditions. Thus, YMC-Triart Prep is equally suitable for industrial scale purifications and in process controlls. Robust Improved particle properties result in better chromatographic performance Flexible Chemical stability ph 1-12 Durable against alkaline CIP Economic Purify more endproduct on less stationary phase Application Data by courtesy of YMC Co., Ltd.
11 Ordering information YMC-Triart Prep, preparative bulk media Pore size (nm) 12 YMC-Triart Prep C18-S Particle size (µm) Product Code 10 TAS12S11 Pore size (nm) YMC-Triart Prep C8-S Particle size (µm) Product Code 10 TOS20S11 15 TAS12S16 20 15 TOS20S16 20 TAS12S21 20 TOS20S21 Typical pack sizes: Laboratory scale: 100 g - 5 kg PE bottle Industrial scale: 5 kg - 50 kg in double lined PE bags inside metal drum Larger pack sizes on request Other particle and pore size combinations on request. Examples of existing customised material: TAS08S11, TOS12S11 Other available brochures: YMC*Gel HG-series High grade silica phases for preparative HPLC The Selectivity Company YMC*Gel HG-series brochure General purpose preparative phases on high grade silica base. Available as silica or with C18, C8, C4, C1, phenyl, cyano, amino or diol bonding. Particle size: 10 µm, 15 µm, 20 µm, 50 µm Pore size: 8 nm, 12 nm, 20 nm, 30 nm www.ymc.de YMC-BioPro prep brochure YMC-BioPro IEX materials Polymeric ion exchange material for biochromatography. Available as strong anion- or cation-exchanger. Excellent flow properties and high dynamic binding capacity. Prep Bulk media Extremely economic in industrial scale applications.
Your local distributor: Co., Ltd. YMC Karasuma-Gojo Bld. 284 Daigo-cho, Karasuma Nisiiru Gojo-dori Shimogyo-ku, Kyoto 600-8106 Japan TEL. +81(0)75-342-4515, FAX +81(0)75-342-4550 www.ymc.co.jp Europe GmbH Schöttmannshof 19 D-46539 Dinslaken Germany TEL. +49(0)2064/427-0, FAX +49(0)2064/427-222 www.ymc.de Taiwan Co., Ltd. 3F, No. 1353, Zhongzheng Rd., Taoyuan City, Taoyuan Country 330, Taiwan (R.O.C.) TEL. +886-3-2150-630, FAX +886-3-2150-286 www.ymctaiwan.com America, Inc. 941 Marcon Boulevard Suite 201 Allentown, PA18109 USA TEL. +1-610-266-8650, FAX +1-610-266-8652 www.ymcamerica.com Korea Co., Ltd. #208, Owners Tower, 16-5, Sunae-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-825 Korea TEL. +82-31-716-1631, FAX +82-31-716-1630 www.ymckorea.com India Ltd. CX - 07, 3rd Floor, Lobe - 1, A-Towers, The Corenthum, Plot No- A-41, Sector - 62, Noida - 201301 (U.P.) India. TEL. +91(0)120-4276020 - 25, FAX +91(0)120-4276026 www.ymcindia.com Co., Ltd. Shanghai Rep. Office Far East International Plaza A2404 No. 319 Xianxia Road, Shanghai 200051 P.R. China TEL: +86-21-6235-1388, FAX: +86-21-6235-1398 www.ymcchina.com