www.essenbioscience.com Ion Channel Discovery Services Customer: Customer PO: Essen contact: Date of assay: Customer contact: Essen Quote No: Srini Kanumilli Lab Notebook Ref: Date of Report: LNB-2650 Experiment overview Ten undisclosed compounds were tested for inhibition of the human ether a go-go related gene (herg) K + channel using IonWorks patch clamp electrophysiology. 8-Point concentration-response curves were generated using 3-fold serial dilutions from the maximum final assay concentration shown in the table below. Results summary Compound pic 50 IC 50 (mm) Curve Profile ID No Value 1 95% CI 3 Value 2 95% CI 4 Slope [Max] mm Cell count 5 QC Notes 6 SN00001 1 5.2 0.12 5.9 [4.4-7.6] 1.1 11 30 PASS SN00002 2 6.8 0.08 0.1 [0.1-0.1] 1.4 11 30 PASS SN00003 3 5.8 0.08 1.5 [1.2-1.7] 1.0 11 26 PASS SN00004 4 5.3 0. 5.0 [3.1-7.9] 1.0 11 30 PASS SN00005 5 <5.0 - >11 - - 11 26 PASS 1±7% inhibition at 11µM. SN00006 6 5.3 0.13 5.3 [3.9-7.1] 1.2 11 25 PASS SN00007 7 <5.0 - >11 - - 11 29 PASS 32±3% inhibition at 11µM. SN00008 8 5.0 0.23 10.5 [6.1-18.0] 0.9 11 27 PASS SN00009 9 <5.0 - >11 - - 11 29 PASS 46±9% inhibition at 11µM. SN00010 10 <5.0 - >11 - - 11 21 PASS 38±9% inhibition at 11µM. Quinidine 11 6.0 0.28 1.1 [0.5-2.0] 1.0 11 28 PASS 1 pic 50 (i.e. the negative logarithm of the IC 50 ) and 2 IC 50 values were obtained from a 4 parameter logistic fit of the concentrationresponse data. Greater or less than values indicate where the potency estimates could not be fully resolved due to the limited concentration range tested, or insolubility. In such cases % maximum inhibition values are returned in the notes column. 3 95% Confidence limits for the pic 50 and 4 IC 50 value from the curve fit. 3 Values <0.5 indicate that the pic 50 is resolved within ±0.5 of a log unit with 95% probability. 5 Denotes the number of data points (cells) included in the curve fit. Up to 4 cells per test concentration may pass the cell QC criteria. Thus, for an 8 point concentration response curve a maximum of 32 cells may be obtained. 6 Solubility observations record the visual presence of precipitate in the assay ready compound plate where samples are prepared at 3x the final test concentration. Solubility observations are reported with reference to the final assay concentration in order to flag the possibility of compromised data points. * Essen BioScience, Biopark, Broadwater Road, Welwyn Garden City Hertfordshire, UK AL7 3AX, ) +44 1707 358686 Page 1 of 5
Compound 1 Compound 2 SN00001 Compound 3 Compound 4 Compound 5 Compound 6 SN00005 SN00003 SN00002 SN00004 SN00006 In each graph the log of molar concentration of test compound is plotted against the effect of the compound on the herg current, expressed as a percentage of the pre-compound signal. In this experiment an average of 7±2% signal decay was observed in the time- and vehicle (DMSO) control. Data are normalised for this vehicleresponse such that a value of % = no drug effect. The smaller grey symbols represent data from individual cells. The larger blue points show the mean data at each test concentration. The line of best fit (4 parameter logistic equation) is generated from the individual cell data points. Outlier data points (boxed symbols) were manually excluded from the curve fit. No more than two per curve were excluded, other than where compounds were visibly out of solution. * Essen BioScience, Biopark, Broadwater Road, Welwyn Garden City Hertfordshire, UK AL7 3AX, ) +44 1707 358686 Page 2 of 5
Compound 7 Compound 8 SN00007 SN00008 Compound 9 Compound 10 SN00009 SN00010 Compound 11 Quinidine In each graph the log of molar concentration of test compound is plotted against the effect of the compound on the herg current, expressed as a percentage of the pre-compound signal. In this experiment an average of 7±2% signal decay was observed in the time- and vehicle (DMSO) control. Data are normalised for this vehicleresponse such that a value of % = no drug effect. The smaller grey symbols represent data from individual cells. The larger blue points show the mean data at each test concentration. The line of best fit (4 parameter logistic equation) is generated from the individual cell data points. Outlier data points (boxed symbols) were manually excluded from the curve fit. No more than two per curve were excluded, other than where compounds were visibly out of solution. * Essen BioScience, Biopark, Broadwater Road, Welwyn Garden City Hertfordshire, UK AL7 3AX, ) +44 1707 358686 Page 3 of 5
Methods summary Electrophysiological recordings were made from a Chinese Hamster Lung cell line stably expressing the full length herg channel. Single cell ionic currents were measured in the perforated patch clamp configuration ( µg ml -1 ) amphotericin) at room temperature (21-23⁰C) using an IonWorks Quattro instrument. The internal solution contained (mm): KCl, 1 MgCl 2, 1 EGTA, HEPES and was buffered to ph 7.3. The external solution contained (mm): 138 NaCl, 2.7 KCl, 0.9 CaCl 2, 0.5 MgCl 2, 8 Na 2 HPO 4, 1.5 KH 2 PO 4 also buffered to ph7.3. Cells were clamped at a holding potential of -70mV for 30s and then stepped to +40mV for 1s. This was followed by a hyperpolarising step of 1s to -30mV to evoke the herg tail current. This sequence was repeated 5 times at a frequency of 0.25Hz. Currents were measured from the tail step at the 5th pulse, and referenced to the holding current. Compounds were then incubated for 6-7 minutes prior to a second measurement of the herg signal using an identical pulse train. Quality control The following QC conditions were applied: (1) Individual cells with any of the following properties were excluded from subsequent analysis (1) seal resistances <50MΩ (2) herg currents <150pA (3) seal resistances that changed by >50% during the experiment (2) A minimum of 17 cells were required for each pic 50 curve fits (3) pic 50 curve fits with a 95% confidence limit of > ±0.5 log were failed (4) Entire assay plates in which the pic 50 of the standard compound (quinidine) was outside of the normal range [5.6-6.3] were failed. * Essen BioScience, Biopark, Broadwater Road, Welwyn Garden City Hertfordshire, UK AL7 3AX, ) +44 1707 358686 Page 4 of 5
SIGNED BY: POSITION: DATE: SIGNATURE: For and on behalf of Essen BioScience Ltd. * Essen BioScience, Biopark, Broadwater Road, Welwyn Garden City Hertfordshire, UK AL7 3AX, ) +44 1707 358686 Page 5 of 5