columns IonPac SCS Silica Cation Separator The SCS Silica Cation Separator is designed for use with nonsuppressed conductivity detection, or single-column ion chromatography (SCIC). This column is particularly suited for analysis of the common inorganic cations, ammonium, select alkanolamines, and transition metals such as zinc and copper. The SCS is recommended when extended calibration linearity for ammonium or alkanolamines is required. The IonPac SCS is targeted for applications in the power generation, chemical, petrochemical, and environmental industries.
Unique Carboxylate Cation Exchanger The IonPac SCS Silica Cation Separator is a unique hydrophilic, lowcapacity weak cation exchanger designed for cation determinations using nonsuppressed conductivity detection. Figure shows the bonded phase bead structure of the IonPac SCS. The substrate for the IonPac SCS is silicabased poly(butadiene-maleic acid) with a particle diameter of. µm. The substrate bead is coated with a unique carboxylic acid functionalized layer. The SCS can be used with eluents of ph 7 and is compatible with typical organic solvents (such as acetonitrile and acetone) at concentration levels up to %. Note: Alcohols should be avoided. Determination of the Common Inorganic Cations plus Ammonium and Ethanolamine Figure A illustrates the separation of the common inorganic cations plus ammonium and ethanolamine using the recommended operating conditions. When using mm methanesulfonic acid at elevated temperature ( C) coupled with nonsuppressed conductivity detection, these analytes can be separated in approximately 8 min (analytical column only). Figure B shows the separation of the common inorganic cations plus ammonium and ethanolamine using the guard and analytical column. The analysis time is approximately min when the guard is added to the IC system. Determination of Trace Sodium in High Ethanolamine An important application for the power industry is the ability to determine trace concentrations of sodium in the presence of high concentrations of ethanolamine. Figure shows a simulated sample that contains ppb ethanolamine spiked with. ppb sodium. A sample volume of. ml was loaded onto a TCC-LP concentrator column using a DXP single-piston pump. The sodium peak is well resolved from the ethanolamine using mm MSA eluent on the SCS column. Carboxylated Functional Layer Figure. Structure of the IonPac SCS Silica Cation Separator packing particle.,, 6 7 Silica Particle Core Diameter:.-µm silica core Surface area: m /g Pore size: Å Column: See chromatograms Eluent: mm methanesulfonic acid Flow Rate:. ml/min Inj. Volume: µl Peaks: A B. Lithium.. mg/l (ppm). Sodium.6. Ammonium... Ethanolamine.. Potassium. 6. Magnesium.. 7. Calcium. Figure. Determination of the common inorganic anions plus ammonium and ethanolamine using the IonPac SCS.,79,8 (A) IonPac SCS, mm 6 7 (B) IonPac SCG, SCS, mm Conc. Column: IonPac TCC-LP, mm Eluent: mm methanesulfonic acid Flow Rate:. ml/min Inj. Volume: ml Peaks:. Sodium. µg/l (ppb). Ammonium. Ethanolamine Figure. Determination of trace sodium in high ethanolamine using the IonPac SCS. 6 987 988 97
Determination of Trace Ammonium in High Sodium Determining trace concentrations of ammonium in the presence of high concentrations of sodium is an important application for the environmental industry. Figure illustrates the separation of trace-level ammonium in the presence of high sodium using mm MSA eluent on the SCS column. The maximum ratio determined for this column is,: sodium to ammonium. The IonPac CS6 column with suppressed conductivity detection is recommended for larger concentration ratios of sodium and ammonium. The IonPac SCS can be used for regulatory compliance monitoring of alkali and alkaline earth cations and ammonium in water and wastewater. The SCS meets or exceeds performance requirements of ASTM Method D699- (see method for further details). Determination of Alkanolamines and the Common Inorganic Cations in Chemical Process Solutions Alkanolamines, including monoethanolamine, diethanolamine, and triethanolamine, are most commonly used individually but are also used in combination to optimize scrubber treatment efficiency for specific chemical processes. In large plants, different alkanolamines may be used in adjacent units to accomodate different scrubbing requirements. The SCS has unique selectivity for alkanolamines and therefore can be used to resolve mixtures of these priority scrubber amines using a mm MSA eluent at elevated temperature, as illustrated in the Figure. Note under these conditions, potassium and diethanolamine are not baseline resolved. Alternatively, by adding % acetonitrile to the mm MSA eluent, these analytes can be resolved in approximately min, as illustrated in Figure 6.,7,77 6 8 6 8 Eluent: mm methanesulfonic acid Flow Rate:. ml/min Inj. Volume: µl Peaks:. Sodium mg/l(ppm). Ammonium. Figure. Determination of low concentrations of ammonium in high concentrations of sodium on the IonPac SCS column.,,8 Eluent: mm methanesulfonic acid Flow Rate:. ml/min Inj. Volume: µl Figure. Determination of alkanolamines using the IonPac SCS. 97 98 6 7 8 6 7 8 9 9 97 Peaks:. Lithium. mg/l (ppm). Sodium. Ammonium.. Monethanolamine. Potassium 6. Diethanolamine 7. Triethanolamine 8. Methyldiethanolamine 9. Magnesium.. Calcium Figure 6. Determination of alkanolamines and diisopropylamine using the IonPac SCS. 976 Eluent: mm methanesulfonic acid/% acetonitrile Column: Temperature: AminoPac C PA ( x mm) Flow Rate: Eluent: Inj. Volume:. ml/min NaOH µl gradient with a NaOAC gradient Detection: Flow Rate: Nonsuppressed conductivity. ml/min Detection: Peaks:. Pulsed Lithiumelectrochemical. mg/l (ppm) detection, gold electrode. Sodium Sample:. Fermentation Ammonium broth.. Monoethanolamine. Diethanolamine 6. Potassium 7. Triethanolamine 8. Methyldiethanolamine 9. Magnesium.. Diisopropylamine. Calcium 978
Determination of Alkanolamines, Transition Metals, and the Common Inorganic Cations in Simulated Feed Water Figure 7 illustrates an example of a -ml simulated feed water sample containing 7 ppm ethanolamine spiked with sub-ppm levels of common inorganic cations, diethanolamine, zinc, cobalt, and manganese. To achieve this separation, the. mm MSA eluent was modified with.8 mm oxalic acid to obtain the appropriate selectivity for this transition metal separation. All cations are well separated with the exception of magnesium and manganese. Determination of Transition Metals and the Common Inorganic Cations Figure 8 shows the separation of the six transition metals and common inorganic cations in a single analysis at the ppm concentration level using a -µl loop injection. An optimized eluent of. mm tartaric acid and. mm oxalic acid is used to achieve the selectivity shown in this example. Also, using these eluent conditions, magnesium and manganese are well resolved.,77,86 Conc. Column: IonPac TCC-LP, mm Eluent:. mm MSA/.8 mm oxalic acid Flow Rate:. ml/min Inj. Volume: ml Peaks:. Lithium.76 µg/l (ppb). Sodium 6.9. Ammonium 6.9. Ethanolamine 7. Potassium.6 6. Diethanolamine 6.9 9+ 7. Zinc 7. 8. Cobalt 6.6 6 7 8 9. Magnesium 9.. Manganese 6.9. Calcium 7. 97 Figure 7. Determination of alkanolamines, transition metals, and the common inorganic cations using the IonPac SCS. 87. 88. 6 7 8 9 Eluent: mm tartaric acid/ mm oxalic acid Flow Rate:. ml/min Inj. Volume: µl Peaks:. Copper mg/l (ppm). Lithium.. Sodium. Ammonium.. Nickel 6. Potassium 7. Zinc 8. Cobalt 9. Manganese. Magnesium.. Cadmium. Calcium 97 Figure 8. Determination of transition metals and the common inorganic cations using the IonPac SCS. TABLE. LINEARITY AND MDLs USING SUPPRESSED CONDUCTIVITY DETECTION a Range Linearity Calculated MDL c MDL Standard Analyte (mg/l) (r ) (µg/l) (µg/l) Lithium. 8.9999.9 Sodium..9999.8 Ammonium b..999. Potassium. 8.9999.6 Magnesium. 8.9999. Calcium. 8.9998.9 a Dionex ICS- system with a -µl injection. b Quadratic fit. c MDL = t s,99 where t s,99 =. for n = 7.
Performance Comparison of Nonsuppressed vs Suppressed Conductivity Detection Tables and summarize the calibration data and method detection limits (MDLs) obtained for the common inorganic cations plus ammonium using the IonPac CS6 and SCS columns, respectively. Tables and summarize the recommendations for use of nonsuppressed vs suppressed conductivity detection. For further information on the comparison of suppressed vs nonsuppressed conductivity detection for the analysis of common inorganic cations, specific alkanolamines, and transition metals, refer to Application Notes 7 and 8. For regulatory methods that require a linear fit for ammonium or weak amines, the IonPac SCS Silica Cation Separator is the recommended column. For further information on this topic, refer to Application Note 7. Requirement TABLE. NONSUPPRESSED VS SUPPRESSED CONDUCTIVITY DETECTION RECOMMENDATIONS Sensitivity/Lowest MDLs TABLE. LINEARITY AND MDLS USING NONSUPPRESSED CONDUCTIVITY DETECTION a Range Linearity Calculated MDL b MDL Standard Analyte (mg/l) (r ) (µg/l) (µg/l) Lithium..9999. Sodium..9999.8 Ammonium..9999.9 Potassium..9999. Magnesium..9999 9.6 Calcium..9999 6.6 a Dionex ICS- system with a -µl injection. b MDL = t s,99 where t s,99 =. for n = 7. Ammonium/Amine Linearity Recommended Mode Suppressed Conductivity Nonsuppressed Conductivity Inorganic Cation Linearity Transition Metals High-Ionic-Strength Samples Suppressed Conductivity Nonsuppressed Conductivity Suppressed conductivity TABLE. LINEARITY AND MDLs USING NONSUPPRESSED CONDUCTIVITY DETECTION A Nonsuppressed Suppressed Feature Conductivity Detection Conductivity Detection Linearity for Up to orders of Over orders of Inorganic Cations magnitude magnitude Linearity for Weak Bases, Linear Nonlinear including Ammonium and (use quadratic curve) certain Amines MDLs for Cations ~ 7 µg/l ~..6 µg/l Sample ph Range Up to mm H + Up to mm H + (ph ) (ph ) Gradient Elution Not possible Commonly used Choice of Eluent Strong/weak acids Strong acids (only low conc.) (high conc. possible) No EG EG Compatible Sample Ionic Strength Low High Detection of Yes (common cations No Transition Metals may interfere)
IC System Operational Requirements The IonPac SCS Silica Cation Separator (-mm i.d.) is recommended for operation using an ICS- (with heater option), ICS- or ICS- IC systems. The IonPac SCS (-mm i.d.) is recommended for operation using the ICS- IC system or the DX-8 Process Analyzer. Eluent Delivery The SCS Silica Cation Separator should be used isocratically with handprepared bottled eluents. The use of the EG/EG Eluent Generator with this column is not recommended, because noise will be higher than hand-prepared bottled eluents. Applications that require eluent gradients or proportioning from two or more eluent bottles are also not recommended. Without the use of a suppressor, the background shift and the noise will be quite significant, making quantification of the analytes difficult. Temperature Control The SCS Silica Cation Separator can be used without long-term loss of performance at C with a mm methanesulfonic acid (ph.) eluent. We recommend not operating the SCS at temperatures higher than C. IonPac Mixer An IonPac Mixer is required for use with the SCS column. The IonPac Mixer is placed before the eluent inlet of the injection valve. The mixer averages any eluent concentration changes due to temperature fluctuation or pump pulsations. The eluent is mixed in this device before reaching the column and reduces the background noise by approximately two-fold. Dimensions: IonPac SCS Silica Cation Separator: mm IonPac SCS Silica Cation Guard: mm IonPac SCS Silica Cation Separator: mm IonPac SCS Silica Cation Guard: mm Maximum Operating Pressure: psi Mobile Phase Compatibility: Acidic eluents (ph 7), % HPLC solvents, alcohols should be avoided Substrate Characteristics: Bead Diameter (µm):. µm (Silica) Pore Size: Å SPECIFICATIONS ORDERING INFORMATION Ion-Exchange Group: Grafted carboxylic acid Functional Group Characteristics: Medium hydrophobic Capacity (µeq/column): mm analytical column: 8 µeq/col mm guard column: 6 µeq/col mm analytical column: 8 µeq/col mm guard column: 6 µeq/col Column Construction: PEEK with - threaded ferrule-style end fittings. All components are nonmetallic. In the U.S., call (8) 6-69 or contact the Dionex regional office nearest you. Outside the U.S., order through your local Dionex office or distributor. Refer to the part numbers listed. Note: Use of SRS with Eluent Suppressors The SCS Silica Cation Separator will contaminate the suppressor and should not be used with an eluent suppressor in-line. IonPac SCS Silica Cation Separator IonPac SCS Silica Cation Separator ( mm)... P/N 6 IonPac SCS Silica Cation Guard ( mm)...p/n 6 IonPac SCS Silica Cation Separator ( mm)... P/N 6 IonPac SCS Silica Cation Guard ( mm)...p/n 6 TCC-LP Cation Concentrator Column ( mm)... P/N 67 (for use with -mm and -mm columns) IonPac Mixer, µl...p/n 66 (required for low-noise operation) IonPac is a registered trademark of Dionex Corporation. Printed on recycled and recyclable paper. Dionex Corporation Dionex Corporation Dionex U.S. Regional Offices Dionex International Subsidiaries 8 Titan Way Salt Lake City Technical Center Sunnyvale, CA (8) 77-8 Austria () 66 Belgium () - 9 Canada (9) 8-96 China (8) 8 8 Denmark 6 6 9 9 P.O. Box 6 West South, Suite A Westmont, IL (6) 789-66 France 9 Germany 66-99- Italy (6) 66 Japan (6) 688- Korea 8 6 8 Sunnyvale, CA Salt Lake City, UT Houston, TX (8) 87-6 The Netherlands (6) Switzerland (6) 99 66 United Kingdom (76) 697 988-6 89-8 Atlanta, GA (77) -8 * Designed, developed, and manufactured under an NSAI registered ISO 9 Quality System. (8) 77-7 (8) 97-99 Marlton, NJ (86) 96-69 6 LPN 68 7M / Dionex Corporation