Bio101 Lab Practicum Preparation Labs 1-7 Prof. Patricia Medina
Sketch and label according to our Week 1 outline, include Prokaryotic /Eukaryotic
Phylogenetic Tree of Life Life forms are divided into 3 domains: B A E Each domain is divided into Domain Eukarya is divided into 4 kingdoms: P P F A
Phylogenetic Tree of Life Life forms are divided into 3 domains: Bacteria or Eubacteria Archaea (extremophiles) ------------------------------------------------------------------------- Eukarya Each domain is divided into kingdoms Domain Eukarya is divided into 4 kingdoms: Protista Plantae Fungi Animalia
Phylogenetic Tree of Life Life forms are divided into 3 domains: Bacteria or Eubacteria Archaea (extremophiles) ------------------------------------------------------------------------- Eukarya Each domain is divided into kingdoms Domain Eukarya is divided into 4 kingdoms: Protista Plantae Fungi Animalia Prokaryotes Eukaryotes
Proper Microscopy: From 4x to 10x 1. Secure the slide on the stage using the slide holders 2. Start with stage up, move down (coarse)until you see (oculars) the image, fine focus (fine) 3. Look from the side and bring 10x in place (revolving nosepiece) 4. Fine focus until the image is clear
Storing your Microscope 1. Turn off 2. No slide 3. No objective 4. Stage down and centered 5. Remove cord and store in drawer 6. Protective bag on 7. Arm out in proper cabinet
Animal vs. Plant cell
Name the kind of cell: Name the staining agent: Name the group, Domain, and the Kingdom
What is the name of this type of cell? Tissue view Teased
Provide 3 characteristics of skeletal muscle cells: M S L
3 characteristics of skeletal muscle cells: Multinucleated Striated Long
What type of cell is shown here? Name of staining agent:
This is a slide showing cells. Has this specimen been stained? Why are the cells green?
What is this?
Identify DNA, histones, chromatin & chromosome
Name the phases of Mitosis
Mitotic Division Prophase Metaphase Anaphase Telophase
Sketch metaphase and label: chromosomes sister chromatids spindle fibers centrioles
Metaphase
Metaphase Spindle fibers Centrioles Sister chromatid
What is happening in Anaphase?
Is this mitosis in plant or animal cells?
Identify the mitotic phases
Are these plant or animal cells? What are these slides showing?
Explain what is happening.
What phenomenon is shown?
Explain the movement of water through the cell in a salty solution.
What reagent tests for the presence of starch? Reaction Result? Reaction Result?
Iodine tests for starch Negative Positive
Name the reagent that tests for glucose. Which contains more glucose?
What phenomenon will be observed?
Name the chemical with the largest and the smallest size. Define diffusion
Use this Osmometer to explain osmosis
What prokaryotic organisms are shown?
ARCHAEA
Bacteria/Eubacteria/Monera
Bacterial cell structure
Antibiotic Susceptibility Test Which is the best antibiotic against the bacteria growing on the plate?
Identify the zone of inhibition, lawn of bacteria, negative control, antibiotic resistance
How many complete cells is the picture showing? 7
Oscillatoria Provide the Domain and Group names. Are these photosynthetic?
Nostoc What are heterocysts?
Unicellular Protista Name the Domain & Group of this Kingdom http://livingkrb. wikispaces.com /Kingdom+Proti sta
Name this protist. What structure allows movement?
These cells are called:. Name their kingdom and domain. Which provides more atmospheric oxygen, plants or these cells?
Provide the phylum name: Provide 3 basic characteristics: Name the two plant structures or generations?
Provide the phylum name: Provide 3 basic characteristics: Identify the plant Structures or generations
Provide the phylum name: Provide 3 basic characteristics: Identify the male and female cones
Provide the phylum name: Provide 3 basic characteristics: Identify the flower parts
Angiosperms Monocots Dicots Number of cotyledons Location of Veins Arrangement of vascular bundles Number of petals
Angiosperms Monocots Dicots Number of cotyledons 1 2 Location of Veins Arrangement of vascular bundles Parallel Branched Scattered Ring Number of petals x3 X4 or x5
Place the following terms in the correct group: Ascus (sac of spores), Yeast, Penicillium, Sporangium (sporeforming body), bread mold & mycorrhizae, Deuteromycetes, Basidiocarp (produces spores) Mushrooms, bracket fungus, Geaster, Coprinus
Which picture corresponds to the definition of Hyphae and which to Mycellium?
For each animal provide: Phylum? Type of Symmetry? Cephalization? Diplo/Triploblastic or No tissues? Parasite? Complete digestive tract? Hermaphrodite?
Provide the name of the phylum, structures shown, location of the worm in the human host?
Label the external anatomy of the earthworm A
Label the external anatomy of the earthworm
Name the parts of the dissected earthworm. http://www.uwlax.edu/biology/zoo-lab/lab-7--annelids-and-smaller-ecdysozoans Click on #16
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