IN QUALITATIVE ANALYSIS,

IN QUALITATIVE ANALYSIS, YOU VE ALWAYS HAD TO CHOOSE BETWEEN ACCURACY AND THROUGHPUT. NOW THERE S NO REASON TO ever have to. WATERS QUALITATIVE ANALYSIS solutions

Waters ACQUITY UPLC System with the Waters Micromass LCT Premier Mass Spectrometer. THE CHALLENGE OF QUALITATIVE ANALYSIS In routine qualitative analysis, rigorous analytical requirements must be met to confidently confirm identities of knowns, rapidly identify unknowns, and characterize complex sample components. LC/MS has long been the technique of choice. Until now, lab scientists had not been able to achieve the analytical system performance demands that would allow the full potential of this technology to be reached. The expectation to produce quality data in less time impacts scientists in a variety of industries, including impurity profiling, trace analysis and environmental monitoring. Scientists are forced to balance the need for comprehensive sample analysis with the demand for more productivity. However, many current high throughput LC/MS approaches fail to achieve the necessary balance and often result in analyte co-elution, ion suppression and false positives. All of which hinder the quality of analysis and make data interpretation especially difficult. WATERS COMPLETE SYSTEM SOLUTION Today, Waters creates a complete system solution that conquers the challenges of qualitative analysis like no other LC/MS system can. Our solution combines the revolutionary chromatographic advances in the Waters ACQUITY Ultra Performance LC (UPLC ) System with the exact mass capabilities of the Waters Micromass LCT Premier Mass Spectrometer, in addition to advanced ACQUITY UPLC chemistries, specialized application managers, and comprehensive services and support. The combination is a first-of-its-kind integrated system that provides high resolution, high sensitivity analyses and gives scientists complete confidence in their qualitative results.

UPLC ULTRA PERFORMANCE LC: A REVOLUTION IN SEPARATION SCIENCE Better chromatographic resolution without sacrifices in speed and sensitivity is fundamental to qualitative analysis techniques. The Waters ACQUITY UPLC System incorporates innovative column chemistries, robust hardware and easy-to-use software. This revolutionary technology optimizes the potential of high-pressure sub-2 micron technology with the low dispersion fluidics and high-speed detectors necessary for successful chromatographic analysis. This makes the ACQUITY UPLC System ideally suited as an inlet to the LCT Premier Mass Spectrometer. The ACQUITY UPLC System s design characteristics enable three times the peak capacity produced by traditional 3.5 µm HPLC columns. This increased resolution reduces the effects of ion suppression, leading to enhanced sensitivity levels. Alternatively, the ACQUITY UPLC System can also be operated with the same resolution and sensitivity as standard chromatography, but with up to a 10x increase in throughput. With either approach, the ACQUITY UPLC System s unparalleled efficiency gives rise to sharper peaks, better resolution, greater sensitivity and more productivity. All of which makes the ACQUITY UPLC System ideal for varying demands of qualitative analysis and method development where enhanced resolution is critical for quality results. HPLC 2.1 X 150mm, 5 µm Rs (2,3) = 4.29 UPLC 2.1 X 50mm, 1.7 µm Rs (2,3) = 4.28 8X Speed 3.4X Sensitivity Same Resolution UPLC 2.1 X 100mm, 1.7 µm Rs (2,3) = 6.38 4.5X Speed 2X Sensitivity 1.5X Resolution Whether your objective is maximum speed (with the same resolution) or maximum resolution (in less time), UPLC allows you to achieve both in addition to increased sensitivity without compromising chromatographic integrity.

LCT Premier EXACT MASS IDENTIFICATION Complementing the ACQUITY UPLC System s superior chromatographic performance, the LCT Premier orthogonal time-of-flight mass spectrometer brings unrivalled sensitivity, reliability and mass accuracy for complex mixture analysis. The LCT Premier s exact mass capabilities enable you to identify the elemental composition of compounds with greater accuracy (sub-3 ppm). When combined with LCT Premier s high resolution (>10,000 FWHM), you can confidently identify compounds and determine elemental compositions. In addition, LCT Premier s new transfer optics offer improved functionality, resulting in superior MS sensitivity across the entire mass range. LCT Premier s positive/negative ion mode switching and Dynamic Range Enhancement (DRE) allow more data to be acquired in one analytical run, thus ensuring mass accuracy over a wide concentration range. With its market-leading capabilities, the easy-to-use LCT Premier gives you more significant information and a more comprehensive analysis for greater confidence in your results. Together with the ACQUITY UPLC, the combined power creates a truly optimized system platform for high-performance exact mass UPLC/MS for qualitative analysis. Enhanced spectral resolution provided by the LCT Premier allows an exact mass measurement to be performed with confidence. In this example, the LCT Premier s narrower spectral peaks yield superior specificity and selectivity, while the quadrupole instrument does not have sufficient spectral resolution to differentiate between sulfacetamide, the compound of interest, and a contaminant ion.

Source Technologies VERSATILITY AT YOUR FINGERTIPS For the highest confidence in your analytical UPLC/MS data, you need the right tools for the job. With Micromass MS technologies, Waters provides an array of easily configurable ionization and source capabilities with LCT Premier. These technologies are designed to maximize instrument uptime and provide versatility for rapid analysis of a wide range of compound classes: ZSpray Dual Orthogonal Source The most robust API ion source available for MS Provides maximum uptime even with dirty samples for maximum productivity Easy tuning of probe types ESCi Multi-Mode Ionization Source Alternating ESI and APCI from single probe Ideal for screening applications Increased ionization coverage for a wider range of compounds LockSpray Dual Electrospray Source Automatic, dedicated exact mass No need for teeing in post-column reference mass solution, removing plumbing issues No ion suppression of reference mass No change in ionization of reference with LC gradient Every spectrum is validated with a reference, removing issues associated with environmental variables

Application Managers OPTIMIZE YOUR QUALITATIVE DATA ANALYSIS Waters MassLynx Software has an array of data processing tools and specialized application managers for your qualitative solutions. Each application manager is individually designed and optimized for your specific data analysis and processing requirements. Waters informatics solutions eliminate the need for complex, time-consuming manual data review, so you can increase your productivity and be sure of your results. ChromaLynx Application Manager For semi-quantitative complex mixture analysis and simplified data mining Detection and location of the maximum number of real components in a sample Carries out comparisons between a real sample and a blank to help determine common and unique components Results are displayed in an easily viewable browser format OpenLynx Application Manager Easy-to-use, wizard-driven walk-up multi-user system operation for varying expertise levels Customizable batch processing Quality control capabilities to ensure satisfactory system operation Exact mass measurement made easier. With MassLynx Software's Elemental Composition Calculator and the new i-fit/filtering technology (turned off on the left and on on the right), the ability to intuitively simplify results has been provided to ensure straightforward data interpretation and accuracy in your decision making.

Waters: For Complete Confidence Waters Corporation is the world s leading provider of instruments, chemistries, software, and support services for chromatography, mass spectrometry, thermal analysis and laboratory informatics. For more than 45 years, Waters has been supplying scientists with the analytical tools that allow them to better understand the secrets of chemistry and of life itself. Around the globe, Waters systems and solutions are used by pharmaceutical, life science, proteomics, food and beverage, environmental, clinical, industrial, and biotechnology researchers to push the boundaries of analytical science. To learn more visit www.waters.com

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