THE STUDY ON FACTORS AFFECTING TIE SURVIVAL OF ESCHEHCHU COLI IN DREDGED MARINE SOILS / A &esis.siili.ttted ifi Mfillrnent of the requirement for the award of the D-egree of Master of Civil Engineering Faculty of Civil and Environmental Engineefing Universiti Tun Hussein Om Malaysia SEPTEMBER 20 15
ABSTRACT Dredging work involves a range of marine mils, varying fiom cwse to fine, clean to contaminated. Dredging involves excavation and disposal and both processes could affect the marine environment through release of possible contaminants. Dredged marine soil OMS) samples with different physico-chemical properties from two dredging locations were analyzed for identification of pathogenic kteria, Identification process was a first element in the detection of biological hazards in marine soils. Due to the potential for transmission of diseases, this hazard becomes a major concern as the DMS bas its om values for reuse or recycle purposes. To prevent the needless cast and the involved in assessing several of pathogenic bacteria, the itldicator bacteria, Esc&rfchfcr eoli (E. eolu has been used to assess the level of biologicd con~tion in marine environmenti The effect of natural factors including salinity, ph, marine soil particle she, solar exposure Ad depth of soil were / investigated. The main god is to understand the bacterial survival ability, as an appmach 0 deal with the hamda. Uiider ~e condition without the existence of predatory microorganisms, experiments is performed at nine salinity level; ranging fiom 0 % to 35 %, ph 6 to 9 and nine hours of solar exposure. It was observed that salinity ha8 induced ph changes. The highest survival was observed at higher salinity after 42 days of expasure. Besides salinity, ph also affects the survival. The ph experimental revealed the high survival of E. culi in high ph. High number of E. culi was found in clay rather than silt soil indicated the survival of bacteria was affected by the particle size as well. In lahmtoq mlar exposure experiments, the data dealing with the survival of bacteria showed that the cell reduction was more pronounced when compared to the absence of solar threat. After an extended period of solar exposures, 2-logs of E, colt have been removed. Therefore, based on the result, the bacteria survivability in DMS was af3ktd by the particle size, dmityf ph and solar exposures.
Kerja penggenrkan melibatkin pelbagai jenis tanah m;arin, yang berbeza-beza dari kaar kepada halw, bersih kepi& tercemw. Pmggemkm melibatkin pnggdim dm pelupusan dan kedua-dua proses boleh menjejaskan alam sekitar rnarin melalui pelepsm b W cemw ymg mugkin. Tanah ma& ke&m (DMS) smpel defigm sifat-sifat fiziko-ma yang berbeza dari dua lokasi penggeruwran didisis unhk menged pasti bslktetia patdgea. Pmss pefigenalaui b&eria adalah eleniefi pmma dalam pengesanan bahaya biologi &dam tanah marin. Berdasarkan kepada potensi untuk penyebaran penyakit, h ad ini menjadi fokus kerana DMS mempunyai nilai untuk tujuan digunakan semula atau kikx semda. Un& mengel&an kas ymg sia- sia dm masa yang terlibat da.lam menilai beberapa bakteria pathogen, bakteria penunjuk, Escherichia coli (E.colij telah digmakin untuk menilai tahap pencemaran biologi dalam persekitarm &. Kesan f&or semula jadi termasuk tahap kernasinan, ph, sak mah tanah m e pdedahan solar dm kedalaman tanah telah dikaji. Matlamat utama ildalah wtuk memahami keupaym survival bakteria, sebagai satu pendekatan untuk mamgani hazad. Dalam keadaan tanpa kewujudan mikroorgmisma ~ ~ g s rekspmhen t, di.d- di sembilan tahap kernashan; antara 0 hingga 35 960 ph 6 hingga 9 dm sembilan jam pendedahan solar. Ia adalah diperhatw bahawa kern- telah mmdarong petubw ph. Swivd terthggi diperhatikan di kemasinan tinggi selepas 42 hari pendedahan, Selain kemasinan, ph jugs memberi kern kep* swivd. Eksperitneh ph mendea sw-ivd ymg tinggi E. coli dalam ph yang tinggi. Jumlah tin& E. coli yyang terdapat dalam tanah liat h b h tan& kelom menunjukkan survival Weria terjejas oleh &z i ~ & juga. Dalsun eksperimen pendedahan solar, data yang berkaitan dengan sunrival bakteria menunjukkan bahawa pengurangan sel adalah lebih ketara jika dibandingkan dengau ketidixm aucarnan solar. Selepas temph ymg panjaug penciecbhan mlar, 2- logs daripada E. coli telah dikwangh. Oleh itu, berkkan keputusan, kemmdirian bakteria dalm DMS terjejas oleh saiz zarah, kernasinan, ph dan pendedahan solar.
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