Number of strains/strain Accession Serogroup Country/Province(s)(n) Source ctxb*

Table S1. Strains used in this study Year of isolation Number of strains/strain Accession Serogroup Country/Province(s)(n) Source ctxb* This study 2001 1 - Non-O1/O139 Guangdong(1) Water - 2002 1 - Non-O1/O139 Fujian(1) Water - 2003 2 - Non-O1/O139 Chongqing(2) Water - 2005 1 - Non-O1/O139 Guangdong(1) Water - 2006 51 - Non-O1/O139 Fujian(7),Liaoning(2),Guangdong(41),Jiangxi(1) Water - 2007 57 - Non-O1/O139 Fujian(7),Liaoning(10),Guangdong(40) Water - 2008 102 - Non-O1/O139 Fujian(4),Liaoning(40),Guangdong(55),Hainan(3) Water - 2008 1 - Non-O1/O139 Sichuan(1) Water + 2009 76 - Non-O1/O139 Guangdong(76) Water - 2010 3 - Non-O1/O139 Zhejiang(2),Shandong(1) Water - 2001 1 - O139 Jiangxi(1) Water - 2002 1 - O139 Guangdong(1) Water - 2005 2 - O139 Jiangxi(1),Jiangsu(1) Water - 2006 2 - O139 Jiangxi(1),Guangdong(1) Water - 2008 2 - O139 Jiangxi(2) Water - Reference strains 1910 NCTC_8457(ATCC14033) AAWD00000000 O1 Saudi Arabia Unknown - 1937 M66-2 NC_012578 O1 Indonesia Unknown - 1965 O395 NC_012582 O1 India Unknown + 1968 V52 AAKJ00000000 O37 Sudan Patient + 1975 N16961 NC_002505 O1 Bangladesh Unknown + 1

1978 116063 NZ_APFK00000000 O1 Brazil Patient - 1980 2740-80 AAUT00000000 O1 United States Water - 1980 TM_11079-80 ACHW00000000 O1 Ogawa Brazil Sewage - 1985 12129(1) ACFQ00000000 O1 Inaba Australia Water - 1987 V51 AAKI00000000 O141 USA Patient + 1991 Amazonia AFSV00000000 O1 Brazil Patient - 1993 4260B NZ_AMVL00000000 O139 Bangladesh Patient + 2001 G4222 NZ_ANNB00000000 O1 South Africa: Gauteng Patient - 2001 MZO-2 AAWF00000000 O14 Bangladesh Patient - 2001 AM-19226 AATY00000000 O39 Bangladesh Patient - 2001 MZO-3 AAUU00000000 O37 Bangladesh Patient - 2004 VC35 AMBR00000000 O1 Malaysia Patient - 2010 NHCC-008D NZ_APGC00000000 O1 Bangladesh Patient - 2011 EM-1676A NZ_APFY00000000 O1 Bangladesh Water - Unknown LMA3984-4 NC_017270 O1 Brazil Water - Unknown PS15 AIJR00000000 O106 USA Sediment in Puget Sound, WA - * +, positive; -, negative 2

Table S2. PCR primers used in this study Primer Nucleotide sequence (5' to 3') Amplicon size (bp) Reference ctxb-f GCCGGGTTGTGGGAATGCTCCAAG ctxb-r CATGCGATTGCCGCAATTAGTATGGC rstr ET -F GAGCTAAAATACAGCAACCAATGC rstr ET -R ACTCACCTTGTATTCG rstr class -F TATTGGGATTGTAAACAGCTGTCC rstr class -R ACTCACCTTGTATTCG rstr calc -F TAGCGCGCCATGTTTGAGCG rstr calc -R GGACTCAAGGGGAGCTAGCCGAA rstr-4**-f GCGGAAACTTTAAATACTTAG rstr-4**-r CTGTTTTGTCGCTGAAAAACTTTG rstr-5-f CATGCTGTAAATACACATGCACGC rstr-5-r GCGAGTTTCACTCTAAC rstr6-f CCGATTTGAATTCAAGTATGCAA rstr6-r GCACTCACCTTGTATTCG rstr232-f CACTTTTTGACCGCGAAGCG rstr232-r ATGCGTCCCAACGAATGGAA 536 1 487 2 480 3 456 4 339 5 312 5 362 6 649 7 rstr-18f rstr-18r tcpa72f TAGCGTGCGGAGTCGCGTTG CCGTTCAAACGCCATGCACCA CACGATAAGAAA ACCGGTCAAGAG 557 8 tcpa 477R CGAAAGCACCTTCTTTCACGTTG 451 (El Tor) 9 tcpa647r TTA CCAAATGCA ACGCCGAATG 620 (Classical) tcpa1185-f TGCTGTCCGTGTCGCACCAG tcpa1185-r CGGCAGAGGCCAAAATCGCCT 1185 this study VSP-I VC0175F VC0175R TGGATGCTCTCTTCTTCA CGCTCACTCACTAATACCGAG 2834 VC0178F VC0178R VC0180F VC0180R AGAGGCTTGTTTACTATCAG ATCGGTACTGTCAGGGCT GGATGAGCAAATACAGCTAAC CTAGGAAGAATTTTATCGGC 2053 2283 10 VC0183F VC0183R CAGTAAGAGTGTAGCGTGCC CCTGCACATCGAGATGC 3389 3

4 VC0185F AGGAGGCGTGTAAGTCATAGC 1110 VC0185R AGACCACGAATACCTGCTCC VSP-II VC0490F CGTGAAGGGATATAGGAG 2337 10 VC0490R TGCAGTTGTTGAATGGAC VC0493F AATGCTTCTCAGGGGGGTCTT 3600 VC0493R CGCTCTTCTTTCCACGCTTCA VC0498F AGGTGGTATCGGGCTGGT 4140 VC0498R TGCGGCTGGAATGGAGTCTG VC0502F TCATCAGTTAGCACACGAAC 476 VC0502R GCTATCGTTATACTTGGCG VC0504F CAGCAAAGGCGGAAGAGGTAG 3240 VC0504R AGCCCGAAATGAATCCCAAAA VC0512F CAGTGGCTTCGCAGAGGA 3900 VC0512R CCCTCCACTGCTATTCCG VC0514F TTATGATCCAAGGAGTAGGG 2089 VC0514R AGGCTGAAAAACAACTTGAG VC0516F GTTTTCTGCGTTGTTCGAG 965 VC0516R TCCTGATGTCTCTCTTGCCG VC0517F CCCACTTCTTCCAGAGTATG 1753 VC0517R CGCAGTCACAGCTTAAACAAC Putative accessory virulence genes TTSS(vcsV2)-F ATGCAGATCTTTTGGCTCACTTGATGGG 742 11 TTSS(vcsV2)-R ATGCGTCGACGCCACATCATTGCTTGCT IS1004-F ATTGTCATCCCTAAACCACC 603 IS1004-R AGGCGGTTTTAATATAAGCC ST-F TATTATTTTCTTCAATCGCATTTAGC 206 12 ST-R ATTTAAACATCCAAAGCAAGCTGG hlya-cl-f GAGCCGGCATTCATCTGAAT 738 9 hlya-cl-r CTCAGCGGGCTAATACGGTTTA hlya-et-f GGCAAACAGCGAAACAAATACC 481 hlya-et-r CTCAGCGGGCTAATACGGTTTA msha-f CGCACAATGAGGTTCGCCAAG 512 13 msha-r CCGAAAATTGACCGCCATTATC chxa-f TGTGTGATGATGCTTCTGG 2000 14

chxa-r TTATTTCAGTTCATCTTTTCGC rtxa-f GGGATACAATGCCCTCTGGCA rtxa-r TGGGTTGGCGGTTGGATTTTAC 977 rtxc-f CGACGAAGATCATTGACGAC 265 rtxc-r CATCGTCGTTATGTGGTTGC 15 SXT-F SXT-R MLST adk-f adk-r gyrb-f gyrb-r TCGGGTATCGCCCAAGGGCA GCGAAGATCATGCATAGACC CATCATTCTTCTCGGTGCTC AGTGCCGTCAAACTTCAGGTA GTACGTTTCTGGCCTAGTGC GGGTCTTTTTCCTGACAATC 986 16 572 730 mete-f CGGGTGACTTTGCTTGGT 802 mete-r CAGATCGACTGGGCTGTG mdh-f mdh-r ATGAAAGTCGCTGTTATTGG TAGCTTGATAGGTTGGG 794 17 pnta-f pnta-r GGCCAGCCCAAAATCCT GATATTGCCGTCTTTTTCTT 739 purm-f purm-r GGTGTCGATATTGATGCAGG GGAATGTTTTCCCAGAAGCC 715 pyrc-f pyrc-r ATCATGCCTAACACGGTTCC TTCAAACACTTCGGCATA 709 References list 1. Kumar P, Jain M, Goel AK, Bhadauria S, Sharma SK, Kamboj DV, Singh L, Ramamurthy T, Nair GB. 2009. A large cholera outbreak due to a new cholera toxin variant of the Vibrio cholerae O1 El Tor biotype in Orissa, Eastern India. J Med Microbiol 58:234-238. 2. Davis, B. M., K. E. Moyer, et al. (2000). "CTX prophages in classical biotype Vibrio cholerae: functional phage genes but dysfunctional phage genomes." J Bacteriol 182(24): 6992-6998. 3. Waldor MK, Mekalanos JJ. 1996. Lysogenic conversion by a filamentous phage encoding cholera toxin. Science 272:1910-1914. 4. Davis BM, Kimsey HH, Chang W, Waldor MK. 1999. The Vibrio cholerae O139 Calcutta bacteriophage CTXphi is infectious and encodes a novel repressor. J Bacteriol 181:6779-6787. 5. Mukhopadhyay AK, Chakraborty S, Takeda Y, Nair GB, Berg DE. 2001. Characterization of VPI pathogenicity island and CTXphi prophage in environmental strains of Vibrio cholerae. J Bacteriol 183:4737-4746. 5

6. Li M, Kotetishvili M, Chen Y, Sozhamannan S. 2003. Comparative genomic analyses of the vibrio pathogenicity island and cholera toxin prophage regions in nonepidemic serogroup strains of Vibrio cholerae. Appl Environ Microbiol 69:1728-1738. 7. Maiti D, Das B, Saha A, Nandy RK, Nair GB, Bhadra RK. 2006. Genetic organization of pre-ctx and CTX prophages in the genome of an environmental Vibrio cholerae non-o1, non-o139 strain. Microbiology 152:3633-3641. 8. Wang D, Wang X, Li B, Deng X, Tan H, Diao B, Chen J, Ke B, Zhong H, Zhou H, Ke C, Kan B. 2014. High prevalence and diversity of pre-ctxφ alleles in the environmental Vibrio cholerae O1 and O139 strains in the Zhujiang River estuary. Environmental Microbiology Reports Article first published online: 5 DEC 2013,DOI: 10.1111/1758-2229.12121. 9. Rivera IN, Chun J, Huq A, Sack RB, Colwell RR. 2001. Genotypes associated with virulence in environmental isolates of Vibrio cholerae. Appl Environ Microbiol 67:2421-2429. 10. O'Shea YA, Reen FJ, Quirke AM, Boyd EF. 2004. Evolutionary genetic analysis of the emergence of epidemic Vibrio cholerae isolates on the basis of comparative nucleotide sequence analysis and multilocus virulence gene profiles. J Clin Microbiol 42:4657-4671. 11. Chatterjee S, Ghosh K, Raychoudhuri A, Chowdhury G, Bhattacharya MK, Mukhopadhyay AK, Ramamurthy T, Bhattacharya SK, Klose KE, Nandy RK. 2009. Incidence, virulence factors, and clonality among clinical strains of non-o1, non-o139 Vibrio cholerae isolates from hospitalized diarrheal patients in Kolkata, India. J Clin Microbiol 47:1087-1095. 12. Jagadeeshan S, Kumar P, Abraham WP, Thomas S. 2009. Multiresistant Vibrio cholerae non-o1/non-o139 from waters in South India: resistance patterns and virulence-associated gene profiles. J Basic Microbiol 49:538-544. 13. Singh DV, Matte MH, Matte GR, Jiang S, Sabeena F, Shukla BN, Sanyal SC, Huq A, Colwell RR. 2001. Molecular analysis of Vibrio cholerae O1, O139, non-o1, and non-o139 strains: clonal relationships between clinical and environmental isolates. Appl Environ Microbiol 67:910-921. 14. Awasthi SP, Asakura M, Chowdhury N, Neogi SB, Hinenoya A, Golbar HM, Yamate J, Arakawa E, Tada T, Ramamurthy T, Yamasaki S. 2013. Novel cholix toxin variants, ADP-ribosylating toxins in Vibrio cholerae non-o1/non-o139 strains, and their pathogenicity. Infect Immun 81:531-541. 15. Chow KH, Ng TK, Yuen KY, Yam WC. 2001. Detection of RTX toxin gene in Vibrio cholerae by PCR. J Clin Microbiol 39:2594-2597. 16. Bhanumathi R, Sabeena F, Isac SR, Shukla BN, Singh DV. 2003. Molecular characterization of Vibrio cholerae O139 bengal isolated from water and the aquatic plant Eichhornia crassipes in the River Ganga, Varanasi, India. Appl Environ Microbiol 69:2389-2394. 17. Octavia S, Salim A, Kurniawan J, Lam C, Leung Q, Ahsan S, Reeves PR, Nair GB, Lan R. 2013. Population structure and evolution of non-o1/non-o139 Vibrio cholerae by multilocus sequence typing. PLoS One 8:e65342. 6

Table S3. Characterization of VSP-I/II clusters and their relationship with elements of CTX prophage and TCP genes No. of strain(s) CTX prophage TCP VSP-I VSP-II ctxb rstr tcpa tcpi VC0175 VC0178 VC0180 VC0183 VC0185 VC0490 VC0493 VC0498 VC0502 VC0504 VC0512 VC0514 VC0516 3 -+++---------+--- 5 -+++------------- 1 ++--------------- 1 -+--------------- 1 -----+-++----+--- 1 --------+----+--+ 1 -----+-++-------- 1 --------+-------- 3 ---------++++++++ 2 ---------+++++-++ 1 ---------++--+--+ 1 ---------++-+--++ 1 -------------+--- 1 -------------+--+ 7

Table S4. Correlations of putative virulence genes and CTX prophage/tcp/vsp-i,ii genes. tcpa ctxb rstr VSP-I VSP-II rtxc TTSS SXT chxa msha rtxa ST IS1004 hlya tcpa 1-0.01.891** -0.02.257** 0.056-0.024 0.103-0.019-0.066 0.076-0.033-0.051 0.037. 0.868 0 0.738 0 0.336 0.684 0.076 0.748 0.26 0.191 0.574 0.379 0.524 ctxb -0.01 1.311** -0.007-0.013 0.02-0.027-0.025-0.038 0.013 0.027-0.011 0.055 0.013 0.868. 0 0.907 0.824 0.737 0.641 0.672 0.512 0.83 0.648 0.844 0.346 0.824 rstr.891**.311** 1-0.022.222** 0.001-0.039 0.077-0.042 -.142* 0.036-0.037-0.048-0.046 0 0. 0.707 0 0.986 0.506 0.188 0.478 0.014 0.537 0.528 0.408 0.428 VSP-I -0.02-0.007-0.022 1.250** 0.039 0.098 0.032 0.051 0.025-0.024-0.023 0.052 0.026 0.738 0.907 0.707. 0 0.5 0.094 0.587 0.386 0.667 0.683 0.693 0.374 0.654 VSP-II.257** -0.013.222**.250** 1 0.022.228**.127* -0.043-0.03 0.06-0.044.147* 0.05 0 0.824 0 0. 0.702 0 0.029 0.467 0.611 0.305 0.452 0.012 0.394 rtxc 0.056 0.02 0.001 0.039 0.022 1-0.018 0.08 0.075.256**.350** 0.066 0.087.558** 0.336 0.737 0.986 0.5 0.702. 0.76 0.169 0.202 0 0 0.257 0.137 0 TTSS -0.024-0.027-0.039 0.098.228** -0.018 1-0.076-0.038 0.057.167** 0.001.176** 0.104 0.684 0.641 0.506 0.094 0 0.76. 0.194 0.513 0.325 0.004 0.985 0.002 0.073 SXT 0.103-0.025 0.077 0.032.127* 0.08-0.076 1-0.032 0.045-0.005-0.083 0.004 0.095 0.076 0.672 0.188 0.587 0.029 0.169 0.194. 0.58 0.44 0.925 0.153 0.948 0.105 chxa -0.019-0.038-0.042 0.051-0.043 0.075-0.038-0.032 1.141*.261** -0.051.132*.147* 0.748 0.512 0.478 0.386 0.467 0.202 0.513 0.58. 0.015 0 0.379 0.023 0.012 msha -0.066 0.013 -.142* 0.025-0.03.256** 0.057 0.045.141* 1.208** 0.042 0.095.496** 0.26 0.83 0.014 0.667 0.611 0 0.325 0.44 0.015. 0 0.47 0.103 0 rtxa 0.076 0.027 0.036-0.024 0.06.350**.167** -0.005.261**.208** 1 0.09.395**.488** 0.191 0.648 0.537 0.683 0.305 0 0.004 0.925 0 0. 0.123 0 0 ST -0.033-0.011-0.037-0.023-0.044 0.066 0.001-0.083-0.051 0.042 0.09 1-0.101 0.044 0.574 0.844 0.528 0.693 0.452 0.257 0.985 0.153 0.379 0.47 0.123. 0.083 0.452 IS1004-0.051 0.055-0.048 0.052.147* 0.087.176** 0.004.132* 0.095.395** -0.101 1.236** 0.379 0.346 0.408 0.374 0.012 0.137 0.002 0.948 0.023 0.103 0 0.083. 0 hlya 0.037 0.013-0.046 0.026 0.05.558** 0.104 0.095.147*.496**.488** 0.044.236** 1 0.524 0.824 0.428 0.654 0.394 0 0.073 0.105 0.012 0 0 0.452 0. Correlations (Spearman's rho,n=295); **. Correlation is significant at the 0.01 level (2-tailed); *. Correlation is significant at the 0.05 level (2-tailed). 8

Table S5 Characterization of putative accessory virulence genes and relationships with CTX prophage/tcp genes No. of strain(s) tcpa ctxb rstr VSP-I VSP-II rtxc TTSS(vcsC2) SXT chxa msha rtxa ST IS1004 hlya 1 + - + - + + - - + + + - + + 1 + - + - + + - - - - + - + + 1 + - + - + + - + - + + - - + 1 + - + - - + + - + + + - - + 2 + - + - - + - + - + + - - + 1 + - + - - + - - - + + - - + 1 + - + - - + - - - + + - + + 1 - + + - - + - - - + + - + + 1 - - + - - - - - - - - - - - 1 - - - + + + + - - + + - + + 1 - - - + + + - + - + - - - + 1 - - - + - + + - + + + - + + 1 - - - + - + - - + + + - + + 2 - - - - + + + + - + + - + + 1 - - - - + + + - + + + - + + 3 - - - - + + + - - + + - + + 1 - - - - + + - - - + + - + + 1 - - - - + + - - + + + - + + 1 - - - - + - + + - + + - + + 1 - - - - - - + + - + + - + + 1 - - - - - - + - + + + - - + 1 - - - - - - + - - + + - - + 2 - - - - - - + - - + - - + + 4 - - - - - - - - + + + - + + 1 - - - - - - - - + + + - - + 2 - - - - - - - - - + + - + + 2 - - - - - - - - - + + - - + 2 - - - - - - - - - + - - + + 1 - - - - - - - - - + - - - + 6 - - - - - - - - - + - - - - 5 - - - - - - - - - - - - - - 1 - - - - - + + + - + + - + + 1 - - - - - + + - + + + + + + 6 - - - - - + + - + + + - + + 3 - - - - - + + - + + + - - + 1 - - - - - + + - - + + + - + 17 - - - - - + + - - + + - + + 8 - - - - - + + - - + + - - + 1 - - - - - + + - - - + - + + 8 - - - - - + - + + + + - + + 3 - - - - - + - + + + + - - + 1 - - - - - + - + + + - - - + 10 - - - - - + - + - + + - + + 7 - - - - - + - + - + + - - + 1 - - - - - + - + - + - - + + 5 - - - - - + - + - + - - - + 1 - - - - - + - + - - + - - + 1 - - - - - + - - + + + + - + 9

32 - - - - - + - - + + + - + + 1 - - - - - + - - + + - - - + 21 - - - - - + - - + + + - - + 2 - - - - - + - - - + + + + + 6 - - - - - + - - - + + + - + 48 - - - - - + - - - + + - + + 31 - - - - - + - - - + + - - + 23 - - - - - + - - - + - - - + 1 - - - - - + - - - + - - - - 2 - - - - - + - - - - + - + + 1 - - - - - + - - - - + - - + 1 - - - - - + - - - - - - - - 10

Table S6. Characteristics of virulence-associated genes and housekeeping genes from non-o1/o139 strains in this study Strain Serogroup tcpa ctxb rstr VSP-I VSP-II rtxc TTSS SXT chxa msha rtxa ST IS1004 hlya adk gyrb mdh mete pnta purm pyrc ZJ193-1 unknow - - - - - + - + + + + - + + 5 1 32 30 1 1 6 ZJ194-1 unknow - - - - - + - - + + + - + + 5 1 32 30 2 1 6 ZJ197-1 unknow - - - - - + - + - + - - + + 11 1 23 14 2 24 1 ZJ198-2 unknow - - - - - + - + + + + - + + 25 1 23 14 5 1 1 ZJ199-1 unknow - - - - + + + - + + + - + + 11 1 23 18 1 24 3 ZJ203-1 unknow - - - - - + - - - + + - + + 16 28 23 18 10 18 2 ZJ205-1 unknow - - - - - + - - + + + - - + 20 28 23 20 10 1 30 ZJ209-1 unknow - - - - - + - - - + + - - + 20 28 13 20 28 21 15 ZJ217-2 unknow + - + - - + + - + + + - - + 20 28 13 20 28 21 10 ZJ221-1 unknow - - - - - + + - - + + - + + 5 20 6 15 6 21 41 ZJ227-3 unknow + - + - + + - - + + + - + + 5 20 6 12 13 21 7 ZJ229-1 unknow - - - - - + - - + + + - + + 5 33 6 29 13 21 8 ZJ231-1 unknow - - - - - + - - + + + - + + 23 32 27 42 3 21 38 ZJ234-1 unknow + - + - + + - - - - + - + + 33 20 34 31 25 21 1 ZJ341-1 unknow - - - - - + + - + + + + + + 26 26 2 4 16 21 10 ZJ345-1 unknow - - - - + + - - - + + - + + 1 7 8 17 34 1 27 ZJ349-1 unknow - - - - - - - - + + + - - + 1 36 28 34 31 1 20 ZJ351-2 unknow - - - - - - + - + + + - - + 26 18 2 36 31 3 7 ZJ356-1 unknow - - - - - + - - + + + + - + 1 24 28 4 10 8 32 ZJ419-1 unknow - - - - - + - + + + + - - + 34 10 1 22 18 25 40 ZJ421-1 unknow - - - - + + - - + + + - + + 4 22 37 8 21 6 21 ZJ186-3 unknow - - - - - + - - - - + - - + 24 22 17 38 24 3 12 ZJ191-1 unknow - - - - - + - - - + + - + + 26 22 11 19 34 1 27 ZJ269-2 unknow - - - + + + + - - + + - + + 26 22 18 23 4 1 28 11

ZJ278-1 unknow - - - - - + - - - + - - - + 2 40 28 23 28 8 15 ZJ278-2 unknow - - - + + + - + - + - - - + 10 40 39 44 38 15 17 ZJ279-1 unknow - - - - - + + + - + + - + + 32 40 35 14 5 18 1 ZJ280-1 unknow - - - + - + + - + + + - + + 26 20 18 14 6 18 41 ZJ282-1 unknow - - - - - + + - - - + - + + 15 6 18 14 15 8 12 ZJ283-2 unknow - - - - - + - - - + + - - + 20 1 18 30 1 2 6 ZJ287-2 unknow - - - - - - - - - + - - - - 1 28 18 28 10 2 2 ZJ180-1 unknow - - - - - + - - - + + + + + 1 5 18 2 13 7 10 ZJ184-1 unknow - - - - - + + - - + + + - + 1 1 18 30 1 19 5 ZJ242-1 unknow - - - - - + - - + + + - + + 18 1 18 41 1 19 42 ZJ243-1 unknow - - - - - - + - - + - - + + 24 1 9 41 1 19 42 ZJ245-1 unknow - - - - - + - - - + + - + + 27 3 9 27 22 20 4 ZJ247-1 unknow - - - - - + + - + + + - + + 27 3 9 28 10 20 2 ZJ251-1 unknow - - - - - + - - - + + + - + 13 3 20 28 10 20 2 ZJ252-1 unknow - - - - - + - - - + + + - + 14 19 1 37 9 20 38 ZJ259-1 unknow + - + - + + - + - + + - - + 1 12 23 46 12 19 43 ZJ260-2 unknow + - + - - + - + - + + - - + 13 20 16 31 25 19 1 ZJ146-1 unknow - - - - - + - - - - + - + + 6 13 1 32 27 19 1 ZJ147-1 unknow - - - - - + - - - - + - + + 29 4 7 35 17 19 36 ZJ149-1 unknow - - - - - + - - + + + - + + 29 4 3 43 38 23 18 ZJ150-1 unknow - - - - - + - + + + - - - + 32 38 35 43 36 18 24 ZJ157-1 unknow - - - - - - - - - + + - - + 32 20 38 18 25 18 25 ZJ158-2 unknow - - - - - + - + - - + - - + 31 4 38 18 17 18 36 ZJ163-1 unknow - - - - - + - + - + + - - + 1 4 23 13 17 18 36 ZJ172-1 unknow - - - - - + - - + + + - + + 28 16 23 21 16 4 36 ZJ172-2 unknow - - - - - - - - - + - - - - 9 2 23 21 22 4 32 ZJ301-1 unknow - - - - - + - - - + + - + + 13 9 23 33 1 12 36 12

ZJ302-2 unknow - - - - - + - - - + + - + + 1 41 1 33 39 17 19 ZJ258-1 unknow - - - - - - - - - - - - - - 17 27 29 32 27 11 1 MJ-34 unknow - - + - - - - - - - - - - - 4 34 1 33 23 9 11 MJ-36 unknow - - - - - + - - - + + - + + 26 27 22 33 27 10 1 MJ-50 unknow - - - - - + - - + + + - + + 28 27 23 33 14 13 16 MJ-51 unknow + - + - - + - - - + + - + + 21 34 23 33 23 12 11 YL823006 unknow - - - - - + - - - + + - - + 20 37 15 47 37 2 23 YL823014 unknow - - - - - + + - - + + - + + 8 21 23 6 7 9 11 YL823016 unknow - - - - - + - - - + + + - + 26 21 24 15 7 5 39 YL823018 unknow - - - - - + - - - + + + - + 11 8 18 15 11 9 39 YL823019 unknow - - - - - + - - - + + + - + 22 8 9 15 11 2 39 YL823033 unknow - - + - + + + - - + + - + + 1 13 30 40 11 1 9 YL823034 unknow - - - - + + + - - + + - + + 32 13 38 40 8 1 9 YL714013 unknow - - - - - + + - + + + - - + 32 4 9 7 8 1 41 YL0882807 unknow - - - - - + - + - + - - - + 1 39 23 43 35 19 18 YL0882811 unknow - - - - + + + + - + + - + + 26 25 14 45 1 19 18 YL0882828 unknow - - - - - - + - - + - - + + 29 13 3 24 25 23 1 YL0882830 unknow - - - - + + + + - + + - + + 29 25 3 45 1 23 11 SC2008 unknow - + + - - + - - - + + - + + 13 31 3 3 19 3 43 2740-80 O1 + - - - - + + - - + - + - + 5 30 32 1 19 1 43 NCTC_8457(ATCC14033) O1 - - - - - + + + - + - + - + 5 31 32 1 19 1 43 12129(1) O1 Inaba - - - - - + + - - + - + - + 26 35 33 11 23 1 13 TM_11079-80 O1 Ogawa - - - - - + + - - + - + - + 1 13 5 39 26 8 1 Amazonia O1 - - - - - + + + + + - + - + 1 13 5 39 26 8 1 VC35 O1 - - - - - + + - - - - + - + 1 13 5 32 27 8 13 AM-19226 O39 - - - - - + - - - + - + - + 20 13 10 25 8 19 11 G4222 O1 + - - - - + - - - + - + - + 5 30 32 1 19 1 43 13

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