William D. Singer, Ph.D. billsinger@dcccd.edu EDUCATION AND TRAINING University of Texas Southwestern Medical Center; Dallas, Texas 1991-1995 ~ Postdoctoral Studies, Cellular Signal Transduction University of Kansas; Lawrence, Kansas 1987-1991 ~ Ph.D., Biochemistry Washburn University; Topeka, Kansas 1982-1986 ~ B.S., Chemistry (Minor in Mathematics) RESEARCH HISTORY Research Scientist 2008-2013 Research Scientist 2004-2008 Research Associate 1995-2004 Postdoctoral Research Fellow 1991-1995 Doctoral Dissertation Research 1987-1991 Research Assistant 1991 Teaching Assistant 1988 Research Assistant 1985-1986 Department of Pathology (with Dr. Errol C. Friedberg) University of Kansas (with Dr. Richard H. Himes) Dissertation: Studies of the Interaction of Vinca Alkaloids with Tubulin, Microtubules, and Cells in Culture U.S. Antarctic Research Program (Project S-O37) Palmer Station, Antarctica (with Dr. H. William Detrich III) Division of Biological Science University of Kansas, Course Title: Cell and Tissue Culture Laboratory Department of Chemistry Washburn University (with Dr. Clifford A.L. Becker and Dr. Janice Barton)
AWARDS AND PROFESSIONAL SERVICE 1998-Present 2000-2004 Postdoctoral Fellowship The Welch Foundation American Association for the Advancement of Science 1992-1994 National Research Service Award Individual Postdoctoral Fellowship National Institutes of Health (1-F32-GM15359-01) 1990 Newmark Graduate Research Award University of Kansas 1988 Borgendale Graduate Seminar Award University of Kansas 1986 Graduated summa cum laude Washburn University PEER-REVIEWED PUBLICATIONS Increased Dietary Cholesterol Intake Stimulates Mutagenesis of DNA in Polymerase Kappa-deficient Mice Singer, W.D., Osimiri, L.C., and Friedberg, E.C. DNA Repair 12: 817-823 (2013) Recognition of the Activated States of G 13 by the rgrgs Domain of PDZRhoGEF Chen, Z., Singer, W.D., Danesh, S.M., Sternweis, P.C., and Sprang, S.R. Structure 16: 1532-1543 (2008) Structure of the p115rhogef rgrgs Domain-G 13/i1 Chimera Complex Suggests Convergent Evolution of a GTPase Activator Chen, Z., Singer, W.D., Sternweis, P.C., and Sprang, S.R. Nat. Struct. Mol. Biol. 12: 191-197 (2005) Mapping the G 13 Binding Interface of the rgrgs Domain of p115rhogef Chen, Z.*, Singer, W.D.* (*co-first authors), Wells, C.D., Sprang, S.R., and Sternweis, P.C. J. Biol. Chem. 278: 9912-9919 (2003) Endothelin-B Receptors Activate Galpha13 Kitamura, K., Shiraishi, N., Singer, W.D., Handlogten, M.E., Tomita, K., and Miller, R.T. Am. J. Physiol. 276: C930-C937 (1999) p115 RhoGEF, a GTPase Activating Protein for G 12 and G 13 Kozasa, T., Jiang, X., Hart, M.J., Sternweis, P.M., Singer, W.D., Gilman, A.G., Bollag, G., and Sternweis, P.C. Science 280:2109-2111 (1998) Curriculum Vitae William D. Singer, Ph.D. Page 2
Direct Stimulation of the Guanine Nucleotide Exchange Activity of p115 RhoGEF by G 13 Hart, M.J.*, Jiang, X.* (*co-first authors), Kozasa, T., Roscoe, W., Singer, W.D., Gilman, A.G., Sternweis, P.C., and Bollag, G. Science 280:2112-2114 (1998) The Monomeric G-Proteins Rac1 and /or Cdc42 Are Required for the Inhibition of Voltage-dependent Calcium Current by Bradykinin Wilk-Blaszczak, M.A., Singer, W.D., Quill, T., Miller, B., Frost, J.A., Sternweis, P.C., and Belardetti, F.B. J.Neurosci. 17:4094-4100 (1997) Three Distinct G Protein Pathways Mediate Inhibition of Neuronal Calcium Current by Bradykinin Wilk-Blaszczak, M.A., Singer, W.D., and Belardetti, F. J.Neurophysiol. 76: 3559-3562 (1996) G q/11 and PLC-β1 Mediate the Substance P-induced Inhibition of an Inward Rectifier K + Channel in Brain Neurons Takano, K., Yasufuku-Tkano, J., Kozasa, T., Singer, W.D., Nakajima, S., and Nakajima, Y. J. Neurophysiol. 76: 2131-2136 (1996) Induction of Inducible Nitric-oxide Synthase by the Heterotrimeric G Protein G 13 Kitamura, K., Singer, W.D., Star, R.A., Muallem, S., and Miller, R.T. J. Biol. Chem. 271: 7412-7415 (1996) Regulation of Phospholipase D by Protein Kinase C is Synergistic with ADP-ribosylation Factor and Independent of Protein Kinase Activity Singer, W.D., Brown, H.A., Jiang, X., and Sternweis, P.C. J. Biol. Chem. 271:4504-4510 (1996) Resolved Phospholipase D Activity is Modulated by Cytosolic Factors Other than Arf Singer, W.D., Brown, H.A., Bokoch, G.M., and Sternweis, P.C. J. Biol. Chem. 270:14944-14950 (1995) G q and G 13 Regulate NHE-1 and Intracellular Calcium in Epithelial Cells Kitamura, K., Singer, W.D., Cano, A., and Miller, R.T. Am. J. Physiol. 268: C101-C110 (1995) The G Protein G 13 Mediates Inhibition of Voltage-dependent Calcium Current by Bradykinin Wilk-Blaszczak, M.A., Singer, W.D., Gutowski, S. Sternweis, P.C., and Belardetti, F. Neuron 13: 1215-1224 (1994) Purification and Characterization of the Subunit of G 13 Singer, W.D., Miller, R.T., and Sternweis, P.C. J. Biol. Chem. 269: 19796-19802 (1994) Polymerization of Antarctic Fish Tubulins at Low Temperatures: Role of Carboxy-terminal Domains Singer, W.D., Parker, S.K., Himes, R.H., and Detrich, H.W., III. Biochemistry 33: 15389-15396 (1994) Curriculum Vitae William D. Singer, Ph.D. Page 3
Cellular Uptake and Tubulin Binding Properties of Four Vinca Alkaloids Singer, W.D. and Himes, R.H. Biochem. Pharmacol. 43: 545-551 (1992) Binding of Vinblastine to Stabilized Microtubules Singer, W.D., Jordan, M.A., Wilson, L., and Himes, R.H. Molec. Pharmacol. 36: 366-370 (1989) Effect of Solution Variables on the Binding of Vinblastine to Tubulin Singer, W.D., Hersh, R.T., and Himes, R.H. Biochem. Pharmacol. 37: 2691-2696 (1988) Preparation of Tetrakis-(arylisocyanide) trialkylphosphitecobalt(i) Complexes With Sterically-hindered Arylisocyanides Becker, C.A.L., Singer, W.D., and Towler, E.M. Inorg. Chim. Acta 113: 75-79 (1986) REVIEWS AND METHODS Regulation of Rho Guanine Nucleotide Exchange Factors by G Proteins Sternweis, P.C., Carter, A.M., Chen. Z., Danesh, S.M., Hsiung, Y.F., and Singer, W.D. Adv. Protein Chem. 74: 189-228 (2007) Assays and Characterization of Mammalian Phosphatidylinositol 4,5-bisphosphate-sensitive Phospholipase D Jiang, X., Gutowski, S., Singer, W.D., and Sternweis, P.C. Methods Enzymol. 345: 328-334 (2002) Regulation of Eukaryotic Phosphatidlyinositol-specific Phospholipase C and Phospholipase D Singer, W.D., Brown, H.A., and Sternweis, P.C. Annu. Rev. Biochem. 66: 475-509 (1997) ABSTRACTS (POSTERS) Toward the Characterization of Different Forms of DNA Polymerase from Mouse Testis Singer, W.D., Guo, C., and Friedberg, E.C. University of Texas-Southwestern Medical Center, Department of Pathology Retreat (2011) A Small G Protein of the Rho-Rac Family Mediates Inhibition of Neuronal Calcium Currents by Bradykinin Wilk-Blaszczak, M.A., Singer, W.D., Sternweis, P.C., and Belardetti, F. Biophys. J. 70: A128 (1996) G q/11 Mediates Substance P-induced Inhibition of the Inwardly Rectifying K + Current in Nucleus Basalis Neurons Takano, K., Yasufuku-Takano, J., Kozasa, T., Singer, W.D., Sternweis, P.C., Nakajima, S., and Nakajima, Y. Curriculum Vitae William D. Singer, Ph.D. Page 4
Biophys. J. 68: A12 (1995) The G Protein, G 13, Mediates Inhibition of Voltage-dependent Calcium Current by Bradykinin Wilk-Blaszczak, M.A., Singer, W.D., Gutowski, S., Sternweis, P.C., and Belardetti, F. Abs. Soc. Neurosci. 20: 1468 (1994) Polymerization of Antarctic Tubulin at Low Temperatures: Role of C-terminal Domains Singer, W.D., Parker, S.K., Himes, R.H., and Detrich, H.W., III. J. Cell Biol. 115: 35a (1991) The Influence of Solution Conditions on the Binding of Vinblastine to Tubulin Singer, W.D., Hersh, R.T., and Himes, R.H. FASEB J. 2: A1354 (1988) Curriculum Vitae William D. Singer, Ph.D. Page 5