Protein Sequencer PPSQ-31A / 33A C297-E055B

Protein Sequencer PPSQ-31A / 33A C297-E055B

Protein Sequencer PPSQ-31A / 33A Achieving Greater Simplicity and Reliability in the Determination of Amino Acid Sequences

Analytical Stability Excellent baseline stability and retention time reproducibility P. 4 Repeated Use of Mobile Phase Reduced running costs and liquid waste in the analysis of PTH-amino acids using isocratic mode P. 5 Software with Excellent Operability Simple operations and easy-to-use data analysis functions P. 5

Analytical Stability Baseline Stability PPSQ series protein sequencers separate PTH-amino acids isocratically. This improves baseline stability and allows high-sensitivity analysis of PTH-amino acids. Retention Time Reproducibility Isocratic sequence analysis provides more stable retention times. Therefore, peaks detected in previous cycles can be cancelled using substation chromatogram processing, making it easier to identify sequences. 2.00 1.75 1.50 1.25 1.00 0.75 0.50 0.25 mv D E N Q S T H G A Y R M V P -0.25 0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 min W F PTH-AA K I L 10pmol Spot 1 Analysis Examples for Spot 1 (The results for cycle 2 onwards are subtraction chromatograms.) 2D Electrophoresis Image of Proteins Extracted from E. Coli cycle 1 cycle 2 1.0 mv DTT 1.0 mv 0.8 0.8 0.6 S 0.6 T 0.4 0.4 0.2 DPU 0.2 DTT 0.0 0.0 cycle 14 cycle 15 0.50 mv 0.50 mv 0.40 0.40 0.30 0.30 DTT Y 0.20 0.10 L 0.20 0.10 cycle 24 cycle 25 0.50 mv 0.50 mv 0.40 0.40 0.30 0.30 0.20 0.10 E DTT 0.20 0.10 DTT K 4

Repeated Use of Mobile Phase Analysis of PTH-Amino Acids Using Isocratic Mode Performing PTH-amino acid analysis in an isocratic mode in which eluents are recycled to allow repeated use of the mobile phase makes it possible to reduce liquid waste and running costs. Software with Excellent Operability Simple Operations Specialized protein sequencer software incorporating control functions for the reaction unit and HPLC analysis unit makes it easy to perform sequence analysis. Main Window Selection of sequencing schedule Summary display of currently executed cycle and step and estimated completion time Sequence Schedule Window Reagent Control Window Sequence analysis can be started by simply registering the number of analysis cycles, data file names, and other information, and then clicking the [Start] button. When a reagent is replaced, the inside of the reagent bottle is purged with nitrogen. The replacement dates and times of the reagents can be ascertained with a glance, and lot control is possible. PPSQ-31A / 33A Protein Sequencer 5

Simpl e, Eas y - t o- U s e D a t a A na l y s is F u n ctio n s Specialized protein sequencer software makes it simple to perform the reprocessing of chromatograms, the overlay of multiple chromatograms, and the automatic estimation of amino acid sequences, which are required for sequence analysis. R ep ro c e s s in g o f c h ro mato g ra m s re q u ire d fo r d ata a n a ly s is A ut om at ic es t im a ti on of am ino ac id s equences Chromatogram peak integration, printing, and other processes can be performed on a per-sample basis. Displays multiple chromatograms. Analysis cycles can be updated simply by clicking a button. Window allows easy identification of sequences. Calibrates PTH-amino acids. Retention times can be edited simply by selecting a PTH-amino acid and clicking a peak. Automatically generates a report containing analytical parameters, estimated sequences, yield rates, and other information. Au to mati c Seq u e n ce Es ti mati o n If the appropriate settings are entered before analysis starts, sequences are automatically estimated after each cycle is complete. Up to four candidate amino acids are displayed together with their certainty levels. 6 O ver lay of m ult iple c hrom at ogr am s C u s t o m ized Repor t s Yield G r aph D is play Reports containing information such as analytical parameters, estimated sequences, and yield rates are automatically created. Initial and repeated yield rates are calculated and displayed in graph form. Amino acids used for calculations can be freely selected.

Specifications Main Unit Reaction method Reaction time Number of reactors PPSQ-31A/33A Edman degradation PPSQ-31A: 47 min/cycle PPSQ-33A: 48.5 min/cycle PPSQ-31A: 1 PPSQ-33A: 3 Sample immobilization method Glass fiber disk (8 mm dia.) or PVDF membrane Reactor temperature control Room temperature + 10 C to 60 C Converter temperature control Room temperature + 10 C to 70 C Column temperature control Room temperature + 10 C to 60 C Number of reagents/solvents 8 (one is an option) Reagent/solvent delivery method Nitrogen gas pressure Dimensions W650 D500 H520 mm Weight PPSQ-31A: 55 kg Solvent Delivery Module Flow rate setting range Dimensions Weight Detector Wavelength setting range Dimensions Weight Control PC Computer Display LC-20AT 1 to 10 ml/min W260 D420 H140 mm 11 kg SPD-20A 190 to 700 nm W260 D420 H140 mm 13 kg IBM PC/AT or compatible OS: Windows XP, Windows 7 17-inch LCD Note: In the interests of product improvement, these specifications may change without notice. Note: Windows is a registered trademark of Microsoft Corporation in the U.S. and other countries. Startup Kit Part name Quantity Reagents for amino acid sequence analysis (7 types) 1 of each type Glass fiber disks 50 Polybrene reagent 1 Apomyoglobin standard 1 Mobile phase for PTH-amino acids 1 L Separation column for PTH-amino acids 1 Mixed standards for PTH-amino acids 2 ml x 4 Note: The startup kit is for initial installation. To order additional reagents, contact Wako Pure Chemical Industries, Ltd. Other Items Provided by User Installation space Power supply Nitrogen gas Exhaust equipment Desktop: W1,730 D600 H550 mm min. (Weight: Approx. 93 kg) 100 VAC, 1,100 VA, 50/60 Hz Purity: 99.9999% min. A cylinder pressure regulator and gas tubing (10 m) are provided as standard accessories. In order to provide an exhaust for gases produced by waste liquids, an exhaust tube must either be connected to exhaust equipment or led outside. An exhaust tube (20 m) is provided as a standard accessory. Note: The required installation space and power supply may change according to the PC and display used. Standard Configurations PPSQ-31A System Part number Part name 292-31310-XX PPSQ-31A (main unit) LC-20AT SPD-20A PPSQ-30 (control PC set) 292-05684-92 Startup kit PPSQ-31A Single Reactor Offers highly cost-effective performance. PPSQ-33A System Part number 292-31330-XX 292-05684-92 Part name PPSQ-33A (main unit) LC-20AT SPD-20A PPSQ-30 (control PC set) Startup kit PPSQ-33A Triple Reactor (Supports consecutive analysis of 3 samples.) Using a triple reactor makes it possible to save time and effort, and gives greater freedom in the formulation of analysis programs. PPSQ-31A / 33A Protein Sequencer 7

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