Radiation Detection & Dosimetry Using Plant Electrical Potential and Optical Spectra Douglas Janssen 1, Mohammad Islam 2, Wenze Xi 3, David J. Y. Feng 4, Joshua Lay 2, Jerry Gu 5, Fow-Sen Choa 2 1 Greater Grace Christian Academy, 6063 Moravia Park Drive, Baltimore, MD 21206, USA 2 University of Maryland Baltimore County, Dept. of CSEE, 1000 Hilltop Circle, Baltimore, MD 21250 3 Thomas Jefferson National Lab, Newport News, Virginia 23606 USA 4 Department of Electrical Engineering, National University of Kaohsiung, Kaohsiung, 811 Taiwan 5 Marriotts Ridge High School, 12100 Woodford Drive, Marriottsville, MD 21104, USA email: choa@umbc.edu
Previous Work in Plant Electrophysiology Mid-IR laser stimulation of Dionaea muscipula (Venus fly trap) D. Eisen, D. Janssen, X. Chen, F-S. Choa, D. Kostov, and J. Fan, Trap Closure of Venus Flytrap via Mid-IR Stimulation, Biomedical Engineering Society Annual Meeting, paper:p-sat-b-29, Atlanta, GA, Oct. 24-27, 2012. > Multi-wavelength study, laser stimulation of insect and annelid nervous systems. Optical power dependence of system potential spiking in black soldier fly Hermetia illucens.
Previous Work in Plant Electrophysiology Laser stimulation of plant vascular tissue caused system potential changes. Below: 820nm laser illumination, 120 mw, targeting xylem tissue of Acer negundo. Physical / chemical stimuli are known to initiate transient changes in biopotential. Some plants exhibit wavelength and temperature-dependent response. Above: System potential changes in Schefflera arboricola when isopropyl alcohol is added to leaf along conduction pathway.
Research Motivations 1. Plant photosynthetic processes and associated pigments are sensitive to radioactivity. Before After
Research Motivations 2. Changes in plant pigments, such as the ratio of chlorophyll a / b, may be key natural indicators for environmental radiation hazards.
Research Motivations 3. Measuring changes in plant electrophysiology, such as changes in system potential and current, can provide evidence for specific environmental hazards.
Please visit poster # 37 References [1] Burdon-Sanderson J. Note on the electrical phenomena which accompany irritation of the leaf of Dionaea muscipula. Proc R Soc. Lond (1873);21:495 6. [2] Arabidopsis Genome Initiative. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature, 408, 796-815. (2000). doi:10.1038/35048692 [3]Islam, M., Wenze Xi, David J. Y. Feng, Fow-Sen Choa; The electrical response of plants under radiation. Proc. SPIE 9073, Chemical, Biological, Radiological, Nuclear, & Explosives Sensing XV (2014) [4] Volkov AG, editor. Plant electrophysiology. Berlin: Springer; 2006. [5] D.S. Fensom, The bioelectric potentials of plants and their functional significance: Some daily and seasonal changes in the electrical potential and resistance of living trees, Can. J. Bot. 41 (1963) 831 851. [6] P. Morat, J.-L. Le Moue l, A. Granier, Electrical potential on a tree. A measurement of the sap flow? C.R. Acad. Sci. Paris 317 (1994) 98 101. [7] Opritov V.A., Retivin V.G., On the mechanism of propagating excitation in higher plant,. Fiziol Rast 29: 915-924, 1982 [8] D. Eisen, D. Janssen, X. Chen, F-S. Choa, D. Kostov, and J. Fan, Trap Closure of Venus Flytrap via Mid-IR Stimulation, SPIE Photonics West, San Francisco, California, 2-7 Feb. 2013. [9] Janssen, D., Islam, M., Chao, D., Gu, J., Eisen, D., Fow-Sen Choa, Electricity Derived from Plants, (in publication) [10] Hartman E, Influence of light on the bioelectric potential of the bean (Phaseolus vulgaris) hypocotyl hook, Physiol Plant, 33: 266-275, 1975. [11] Dominique Gibert, Jean-Louis Le Moue, Luc Lambs, Florence Nicollin, Frederic Perrier, Sap flow and daily electric potential variations in a tree trunk, Plant Science, 171, p. 572 584, 2006. [12] Blackburn, G.A., Spectral indices for estimating photosynthetic pigment concentrations: a test using senescent tree leaves, Int. Journal of Remote Sensing, (1998), 19, 4, 657-675 [13] Hashimoto et al., X-Ray fluorescent and mid-infrared spectroscopic analysis of tomato leaves, Proc. SICE-ICASE Int l Joint Conference, Busan, Korea, 18-21 Oct. 2006 [14] Jovanic, B.R., Radenkovic, B., Despotovic-Zrakic, M., Bogdanovic, Z., Panic, B., Impact of nuclear radiation on plants photosynthesis and chlorophyll content after bombing with U238 enriched bombs., American-Eurasian Journal of Sustainable Agriculture, 6, 1, (2012) 33-43 This material is based upon work supported by the National Science Foundation under Grant No. EEC-0540832