Parasitology Research Electronic Supplementary Material for: Sarcophaga (Liosarcophaga) tibialis Macquart 1851 (Diptera: Sarcophagidae): micromorphology of preimaginal stages of a fly of medical and veterinary interest Ángela Paños-Nicolás, Mª Isabel Arnaldos, Mª Dolores García, Nicolás Ubero-Pascal Concise Title. Micromorphology of immature stages of S. (L.) tibialis. Affiliation and adresses of authors Area of Zoology. Department of Zoology and Physical Anthropology. Biology Faculty. Murcia University. 30100 Murcia. Spain. Corresponding author Nicolás Ubero-Pascal; nubero@um.es; +34 868 884960 1
Fig. S1 Selected morphological features of the first instar larva of S. (L.) tibialis by light microscopy. a Ventral view of pseudocephalon. b Lateral view of cephalic lobe. c Detail of maxillary palpus, ventral organ and mouthhook. d Central area of cephalopharyngeal skeleton in lateral view. e Central area of cephalopharyngeal skeleton in dorsal view. f-h Central area of cephalopharyngeal skeleton in ventral view at different planes following dorso-ventral axis, ventral tip of anterior labial sclerite plane, intermediate sclerite plane and labrum plane respectively. i Spines of anterior spinose band in dorsal area of ai. j Spines in ventral creeping welt of aiii. k Spines of lateral creeping welt of aiii. l Detail of edge of spiracular cavity. Abbreviations: ads additional sensilla, an antenna, asb anterior spinose band, cc central cluster, ds dental sclerite, is intermediate sclerite, la labrum, ls-a anterior labial sclerite, ls-p posterior labial sclerite, mk mouthhook, mp maxillary palpus, or oral ridge, p1-p4, p7 posterior papillae, pb parastomal bar, pr-mk protuberance of mouthook, shl hair-like spines, vo ventral organ. 2
Fig. S2 Selected morphological features of the first instar larva of S. (L.) tibialis by SEM. a Antenna. b Tips of mouthhook and striae. c Labium. d Spines of dorsal area of anterior spinose band of ti. e Spines of ventral area of anterior spinose band of ti. f Keiling s organs. g Infant anterior spiracle. h Ventral creeping welt of aiii. i Spines of lateral creeping welt of of aiii. j Detail of spines of ventral creeping welts. k Detail of posterior papillae. l Detail of posterior spiracle. Abbreviations: ae non-functional respiratory slit, ans additional sensillum in antenna, as anterior spiracle, br antennal basal ring, dm antennal dome, ko Keiling s organs, ll labial lobe, lo labial organ, mk mouthhook, mo functional mouth opening, or oral ridge, p2-p3 posterior papillae, pls placoid-like sensillum, pt peristigmatic tuft, rs respiratory slit, sc coeloconic sensilla, shl hair-like spines, sp pit sensillum, srt striae. 3
Fig. S3 Interpretative drawings of cephalopharingeal skeleton in the three larval stages. a and b Lateral and dorsal views of sclerite arrangement in the first instar larva. c and d Lateral and ventral views of sclerites arrangement in the second instar larva. e and f Lateral and ventral views of slcerites arrangement in the third instar larva. Abbreviations: db dorsal bridge, dc dorsal cornu, ds dental sclerite, is intermediate sclerite, la labrum, ls-a anterior labial sclerite, ls-p posterior labial sclerite, mk mouthhook, pb parastomal bar, pr-mk protuberance of mouthook, vb ventral bridge, vc ventral cornu, vp vertical plate, w window 4
Fig. S4 Selected morphological features of the second instar larva of S. (L.) tibialis by light microscopy. a Ventral view of pseudocephalon. b Detail of maxillary palpus. c Spines in dorsal area of tii. d-f Central area of cephalopharyngeal skeleton in ventral view at different planes following dorso-ventral axis, ventral tip of anterior labial sclerite plane, intermediate sclerite plane and triangular-shaped protuberances of mouthhook plane respectively. g Anterior spiracle. h Spines in dorsal area of av and avi. i Detail of ventral creeping welt. j Detail of posterior spiracles. Abbreviations: av-avi abdominal segments, ads additional sensilla, an antenna, asb anterior spinose band, ds dental sclerite, is intermediate sclerite, ls-a anterior labial sclerite, ls-p posterior labial sclerite, mk mouthhook, mp maxillary palpus, pb parastomal bar, pr peritremet, pr-mk protuberance of mouthook, psb posterior spinose band, rs respiratory slit, sb1-3 basiconic sensilla of maxillary palpus, sc1-3 coeloconic sensilla of maxillary palpus, sp pit sensillum, asterisk discontinuity of anterior spinose band in dorsal area, vo ventral organ. 5
Fig. S5 Selected morphological features of the second instar larva of S. (L.) tibialis by SEM. a Detail of cephalic lobe. b Detail of antenna. c Ventral view of anterior spinose band of ti. d Spines of latero-ventral area of anterior spinose band of ti. e Spines of dorsal area of anterior spinose band of ti. f Detail of spines in dorsal area of anterior spinose band of tiii. g Lateral creeping welt of segment aiii. h Ventral creeping welt of aiii. i Detail of spiracular cavity of anal division. j Detail of posterior papilla. k Detail of posterior spiracle. Abbreviations: ae non-functional respiratory slit, an antenna, ans additional sensillum in antenna, asb anterior spinose band, asb-2 second branch of anterior spinose band of ti, br antennal basal ring, cw ventral creeping welt, dm antennal dome, ko Keiling s organs, lcw lateral creeping welt, mp maxillary palp, p1-p7 posterior papillae, pe posterior spiracle, pgs membranous-like globular papilla, pls placoid-like sensillum, pt peristigmatic tuft, pr peritreme, rs respiratory slit, rsp ring of pit sensillum in interbands, shl hair-like spines, sl scales, sp pit sensillum, vo ventral organ. 6
Fig. S6 Selected morphological features of the third instar larva of S. (L.) tibialis by light microscopy. a Detail of cephalic lobe. b Spines of ventral are of anterior spinose band of ti. c Detail of anterior spinose band of tiii. d-e Ventral view of central area of cephalopharyngeal skeleton at different planes following dorso-ventral axis, ventral tip of anterior labial sclerite plane and intermediate sclerite plane respectively. f Dorsal view of central area of cephalopharyngeal skeleton. g Ventral view of ventral bridge and intermediate sclerite. h Detail of posterior papillae. i Posterior spiracles. Abbreviations: b buttom, br basal ring, dm antennal dome, ds dental sclerite, fav foremost arm of ventral cornu, is intermediate sclerite, ls-a anterior labial sclerite, ls-p posterior labial sclerite, mk mouthhook, mp maxillary palpus, p4- p7 posterior papillae, pb parastomal bar, pr peritreme, pr-mk protuberance of mouthook, rs respiratory slit, shl hair-like spines, vb ventral bridge. 7
Fig. S7 Selected morphological features of the third instar larva of S. (L.) tibialis by SEM. a Detail of antenna. b Dorsal view of pseudocephalon. c Detail of scales, ventral organ and oral ridge. d Labial lobe. e Detail of tip of mouthhook. f Spines in dorsal area of anterior spinose band of tiii. g Spines in lateral area of anterior spinose band of ti. h Lateral creeping welt. i, spines in dorsal area of aiv. j Detail membranous-like globular papilla. k Detail hair-like spines of spiracular cavity. i Detail of posterior papilla. Abbreviations: aiv-av abdominal segments, asb anterior spinose band, br antennal basal ring, cl cephalic lobe, dm antennal dome, lcw lateral creeping welt, ll labial lobe, lo labial organ, or oral ridge, rsp ring of pit sensillum in interbands, sc coeloconic sensilla, sl scales, sp pit sensillum, vo ventral organ. 8