A Study on Vertically Propagating Tidal and Gravity Waves During Continuous Convections Events over the Equatorial Tropical Region

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[ VOLUME 5 I ISSUE 1 I JAN. MARCH 2018] E ISSN 2348 1269, PRINT ISSN 2349-5138 A Study on Vertically Propagating Tidal and Gravity Waves During Continuous Convections Events over the Equatorial Tropical Region 1,2M. S. Arunachalam 1E.W.R.E. Division, Department of Civil Engineering, I.I.T. Madars, Chennai-600 036 2Department of Physics, Sri Venkateswara University, Tirupati-517502 Received Dec. 06, 2017 Accepted Jan. 07, 2018 ABSTRACT Propagation of tropospheric gravity waves and tides upward into the mesosphere and lower thermosphere (MLT) region during continuous convection events over two equatorial tropical stations have been studied with the help of vertical velocity information from MST Radar, MLT winds from Medium Frequrency (MF) radar at Tirunalveli, Meteor radar at Trivandrum and winds and temperature from European Centre for Medium- Range Weather Forecasts (ECMWF) Re-Analysis data. Short period gravity waves of ~2-4 hr periodicities were noticed (27 th and 29 th September 2004) with increase in amplitude with height within the tropospheric region. Wavelet amplitude spectrum of temperature and meridional wind has shown that the tidal oscillations are strong enough at almost all height regions with increase in amplitude within the troposphere and stratospheric (TS) region. Both tidal and gravity wave amplitudes of MLT winds show almost increasing trend with altitude over Trivandrum and opposite trend at Tirunalveli. Except zonal wind, the meridional wind and temperature profiles in the TS region has shown that the tidal amplitudes are increasing along latitude which is similar to the direction of motion of the convective core as seen from outgoing long wave radiation. The weak tidal amplitudes in the lower stratospheric region are due to the influence of strong planetary wave amplitudes within that region. Meridional wind shows more prominent tidal amplitudes throughout the observational period when compared with zonal wind in the MLT region. Further we have noticed that the amplitude of wind and temperature perturbations increasing with height. Hence energy is conserved in the middle atmosphere for present study. Downward phase propagation was dominant for the entire period of observations leading to upward energy propagation. 1. Introduction: Understanding of tidal variability in the tropospheric and stratospheric (TS) regions and modulation of the wave amplitudes with wave-mean-flow interactions is necessary for the parameterization of tides in the middle atmospheric general circulation models. Tidal waves that are generated in the TS region propagate upward into the middle atmosphere, and they reach their maximum amplitudes and then get dissipated in the lower thermospheric region because of turbulence and molecular diffusion [Ortland and Alexander, 2006]. Induced nonlinear interactions and breaking of waves often takes place in the MLT region with increase in tidal and gravity wave amplitudes with height, and is due to the effect of decrease in background density of atmosphere [Thayaparan et al., 1995]. Hence, tides play a dominant role in the energy balance of the middle atmosphere. Tidal amplitudes can go beyond 50 m/s and 20 K corresponding to that of horizontal wind and temperature, respectively, with overall dominance comprises of diurnal variations (are substantially dominant around equinoxes than around solstices) and local dominance comprises to that of semidiurnal and terdiurnal tides [McLandress, 2002a; A.K. Smith, 2004]. Tides comprise of high phase speeds and are very less affected by the background atmosphere. For gravity and planetary waves which is quite opposite. The diurnal tides signify the response of the atmosphere to the principal constituent of diabatic heating and insolation, and is one of the prime objective of the present study. It has been reported that the diurnal tide is dominated by zonal wavenumber s=1, that of the migrating diurnal tide at low latitudes [Wu et al., 2006, 2008], which itself is dominated by the upward moving diurnal (1,1) Hough mode, accounting for peaks around 20 30 latitude in both hemispheres in horizontal wind fields[l.c. Chang et al., 2012]. Existence of PWs has been confirmed in the lower, middle and upper atmospheric regions, and contributes extensively to the variability of atmospheric parameters in these regions. PWs show prominent wind amplitudes (>10 ms 1) in the TS region [C. M. Huang et al., 2009]. Vineeth et al., [2011] has shown that the planetary wave-tidal interaction and the subsequent modification of the tidal components are found to be the reason for the wave amplification in the MLT region in the day-to-day variability in the Equatorial Electro Jet (EEJ). Huang et al., [2012] using a combination of bispectral and correlation analyses verified the occurrence of nonlinear interactions among different tidal components such as strong tide- 56 IJRAR- International Journal of Research and Analytical Reviews Research Paper

[VOLUME 5 I ISSUE 1 I JAN. MARCH 2018] e ISSN 2348 1269, Print ISSN 2349-5138 http://ijrar.com/ Cosmos Impact Factor 4.236 gravity wave, tide-planetary wave, and tide-tide interactions in the middle thermosphere. They found that the energy transfer trend changes very frequently during tide-tide interactions, indicating the redistribution of that tidal energy is frequently among different tidal components. In the present study we tried to investigate the effect of modulation of tidal amplitudes by planetary waves in the TS region and tidal-gravity interactions in the MLT region during deep convection events in equatorial tropical regions from 24 th September to 04 th October 2004. 2. Data 2.1. MF radar, Tirunelveli The MLT winds (88-98 km) from the MF radar operated at Tirunelveli (8.71N, 77.81E), India is utilized for the present study. It is operated at frequency of 1.98 MHz. It measures the wind using the spaced antenna technique in the 68 98 km height range during the day time and from 70 km and above during the night time (Rajaram and Gurubaran, 1998). The data acceptance rate (Briggs, 1984) is relatively high at heights above 84 km with the largest acceptance rate at around 88 km. Winds are recorded every 2 min at 2 km height intervals. The present study utilizes the hourly averaged data from the raw winds for every 2-min acquired during 24 th September to 4 th October 2004, when the radar was operated continuously with only a few data gaps, which are linearly interpolated. 2.2. Meteor Radar, Trivandrum MLT winds from all Sky interferometric METeor (SKiYMET) radar from Trivandrum has been utilized for the present study. SKiYMET radar is operated with an operating frequency of 35.25 MHz, with a peak power of 40 kw. In this radar, four three-element Yagi antennas are used for transmission, and five two-element Yagi antennas are used for reception. Detailed experimental description and specifications are given by Kumar et al, [2006]. Various experiments were conducted in the past to understand the MLT dynamics over Trivandrum, but very least information was found during deep convective events. During most of these experiments, MLT winds and temperature measured by these radars will provide unique information about the MLT region. For the present study, MLT winds from the meteor radar, operated round the clock during continuous convection events from 24 th September to 4 th October 2004 has been utilized. The meteor radar provided valuable wind information in the MLT region in the height domain of 82-100 km with a height resolution of 3 km and a time resolution of 1 hour. These datasets are readily used for studying the diurnal/semi diurnal tides, gravity waves and their interaction. 2.3. MST Radar, Gadanki We have collected the vertical velocity information from the VHF radar (53MHz) located over low latitude tropical station Gadanki. Detailed description of MST radar located at Gadanki has been given in Rao et al, [1995]. The MST radar was operated in the vertical direction (Zenith-x) from 27-30 September 2004. Thunderstorm and squall-line events occurred during 27 th and 29 th, respectively. On remaining days there was no continuous wind information in the lower atmosphere over this region. 2.4. ERA-interim data In this paper, we use a few ERA-Interim parameters, namely, zonal wind, meridional wind and temperature, available for the 37 pressure levels from the surface to 1 hpa at the ECMWF (European Center for Medium Range Weather Forecasting) website (http://www.esrl.noaa.gov). These data were prepared by ECMWF using their variational data assimilation system. The ERA-interim data set consists of results from analysis conducted at six-hour intervals (viz., 00UTC, 06UTC, 12UTC and 18UTC) available for a 1.5 o latitude longitude grid. 2.5. NCEP OLR data Un-Interpolated daily outgoing longwave radiation (OLR) data attained by NOAA satellite are accessible from the NOAA s website (http://www.cdc.noaa.gov) for 2.5 latitude longitude grid, were used as a proxy for tropical convection. The OLR data has been explored during 24 th September to 4 th October 2004. 3. Results and Discussions: Convection plays an important role in the generation of waves in the troposphere (source region). In order to notice the convective moment during the observational period we have plotted the NCEP Un-Interpolated OLR for the observational period in the Fig.1. Research Paper IJRAR- International Journal of Research and Analytical Reviews 57

[ VOLUME 5 I ISSUE 1 I JAN. MARCH 2018] E ISSN 2348 1269, PRINT ISSN 2349-5138 Fig.1. Longitude-Latitude variation of NCEP daily un-interpolated OLR for the period of study. From the Fig.1 it can be noticed that there are continuous convective events occurred over and around the region of study with a net movement of convection towards the North-East direction. Among these, 24-30 September 2004 are convection events associated with thunderstorm events and 1-4 October 2004 are deep convection events associated with cyclone (over Bay of Bengal). Fig.2. Wavelet amplitude spectrum of peak vertical velocities (ms -1 ) in the height regions 5-10 km ((a) & (b)), 10-15 km ((c) & (d)) and 18-22 km ((e) & (f)) for 27 September 2004(left panel) and 29 September 2004 (right panel), respectively. We have collected vertical velocity information from MST radar and applied wavelet analysis in order to obtain the time history of peak gravity wave amplitudes. The magnitude of vertical velocities are found to show maximum updrafts of 4.21 m/s and 4.44 m/s weaker compared to downdraft 6.46 m/s and 4.69 m/s on 27 th and 29 th, respectively (which is not shown here). Short period gravity wave (~2-4) 58 IJRAR- International Journal of Research and Analytical Reviews Research Paper

[VOLUME 5 I ISSUE 1 I JAN. MARCH 2018] e ISSN 2348 1269, Print ISSN 2349-5138 http://ijrar.com/ Cosmos Impact Factor 4.236 amplitudes have been noticed from the wavelet amplitude spectrum of vertical velocity fluctuation within the tropospheric region between 10:05:39 hr to 18:06:57 hr on 27 th September (during thunderstorm) and 02:35:17 hr to 10:31:25 hr on 29 th September (during Squall-line passage) has been shown in Fig. 2. The wavelet amplitude spectrum has obtained for middle (5-10 km), upper (10-15 km) troposphere and lower (17-22 km) stratospheric regions, respectively. Time history of peak vertical velocities obtained by taking average over a given height region are plotted here. In the middle troposphere (Fig. 2(a) & 2(b)), there are harmonics with 8 min, 24 min, 48 min and 128 min periodicities on 27 th and 16 min, 24 min, 48 min 80 min and 128 min periodicities on 29 th. In the upper troposphere (Fig. 2(c) & 2(d)), there are harmonics with 20 min, 32 min, 40 min, 64 min and 108 min periodicities on 27 th and 12 min, 16 min, 32 min, 108 min and 240 min periodicity on 29 th. In the lower stratosphere (Fig. 2(e) & 2(f)), there are harmonics with 20 min,32 min and 128 min periodicities on 27 th and 16 min,30 min and 64 min periodicities on 29 th. Hence it may be stated here that these gravity waves are getting filtered or modulated by some other waves which was not clearly discernible here. For further understanding of the effect of filtering or modulation of gravity waves by other sources we have investigated the ECMWF Re-Analysis data sets. Fig.3. Height-Time section of background zonal wind (ms -1 ) (top), meridional (ms -1 ) wind (middle) and Temperature (K) (bottom) in (a-b) and their corresponding fluctuations in (d-f) for the period of study from ERA-Interim Reanalysis data. Figure 3 shows the height-time intensity plots of zonal wind (m/s), meridional wind (m/s), temperature (K) (in (a-c)) along with their fluctuations (in (d-f)) for the present observational period. From the zonal wind it is found that the TEJ strength was getting intensified from ~15 m/s to 28 m/s in the height regions ~15-16.5 km. Both zonal and meridional components are showing increase in intensity with time. Zonal wind fluctuation shows large scale features throughout the observational period. Whereas meridional wind and temperature fluctuations are showing planetary scale features similar to zonal wind fluctuation in the tropospheric regions but in the stratosphere above 20 km there are strong day to day variations were found. In the present study, the zonal wind, meridional wind and temperature fluctuations (Fig.3) in the TS region gave the clue of planetary and tidal waves. However, to confirm the generation of these planetary and tidal waves from the convective cores, their propagation characteristics need to be understood. To study the wave activity in the TS region in response to these continuous convective events, the zonal wind, meridional wind and temperature fluctuations in the TS region are subjected to wavelet analysis. It is an unique tool for analyzing non-stationary and time localized oscillations, is chosen to study the time evolution of planetary and tidal waves. Research Paper IJRAR- International Journal of Research and Analytical Reviews 59

[ VOLUME 5 I ISSUE 1 I JAN. MARCH 2018] E ISSN 2348 1269, PRINT ISSN 2349-5138 Fig.4. Wavelet amplitude spectrum of zonal wind fluctuation for different height levels obtained using ERA- Interim Reanalysis data. Fig.5. Same as Fig.4, but for meridional wind fluctuation. 60 IJRAR- International Journal of Research and Analytical Reviews Research Paper

[VOLUME 5 I ISSUE 1 I JAN. MARCH 2018] e ISSN 2348 1269, Print ISSN 2349-5138 http://ijrar.com/ Cosmos Impact Factor 4.236 Fig.6. Same as Fig.4, but for temperature fluctuation. In order to investigate the time variation of wave periodicities at different height levels we have plotted the (normalized) amplitude spectrum of wavelet for zonal, meridional and temperature fluctuations. From the Figs 4 & 5 (zonal and meridional) it is found that at almost all the height levels diurnal tidal amplitude are dominant along with ~20-40 hr and ~40-60 hr /40-70 hr oscillations. But the amplitude spectrum of temperature (Fig 6) shows that the diurnal tidal oscillations only dominant throughout the observational period with increase in amplitude with height. Compared with amplitude spectrum of zonal and meridional wind, in the temperature spectrum ~20-40 hr and ~40-60 hr /40-70 hr are insignificant with both height and time. Hence from the Figs 4-6 it is clear that the other than tidal oscillations, there are PW periodicities which are mainly due to the effect of background wind in the zonal and meridional components whose effect is least significant as seen from the temperature spectrum (Fig 6). Fig.7. Longitude-Height and Latitude-Height variation of diurnal tide amplitude (top panel) and phase (bottom panel) computed from the zonal wind (ms -1 ), meridional wind (ms -1 ) and temperature (K) fluctuations using FFT analysis for the present observational period. To find the amplitude and phase variation along the latitude and longitude at different height levels, we have subjected the zonal wind, meridional wind and temperature fluctuations to Fast Fourier Transform Research Paper IJRAR- International Journal of Research and Analytical Reviews 61

[ VOLUME 5 I ISSUE 1 I JAN. MARCH 2018] E ISSN 2348 1269, PRINT ISSN 2349-5138 (FFT) analysis. Fig 7 shows the latitude-height and longitude-height section of amplitude and phase of diurnal tides computed using Fast Fourier Transform of zonal, meridional winds and temperature. From the Fig 7 it is seen that the amplitudes of diurnal tide are increasing with altitude along both latitude and longitude. Phase profiles of diurnal tides computed using FFT of zonal wind, meridional wind and temperature fluctuations are distributed at higher local time range, which is greater than 20 h along both latitude and longitudinal directions. Overall, the phases of zonal wind, meridional wind and temperature, exposes higher value throughout the altitude and also along the horizontal direction. A consistent pattern of significant phase coherence (almost same phase) is observed over the region of study at higher values (most prominent in the zonal wind), and it is clearly discernible from the plot. Fig.8. Time variation of zonal (top panel) and meridional wind (bottom panel) at different levels for MFRTV location (represented by different colors) during the present observational period, 62 IJRAR- International Journal of Research and Analytical Reviews Research Paper

[VOLUME 5 I ISSUE 1 I JAN. MARCH 2018] e ISSN 2348 1269, Print ISSN 2349-5138 http://ijrar.com/ Cosmos Impact Factor 4.236 Fig.9. Same as Fig.8, but for MRTM location. To verify whether these oscillations are propagating up into the MLT region we have investigated the MLT winds from two equatorial tropical stations MF Radar, Tirunalveli (MFRTV) and Meteor Radar, Trivandrum (MRTM). Fig 8&9 shows the time-height section of zonal and merdional for both the regions. It is found that the zonal wind is showing maximum of ~70 m/s to -80 m/s in the eastward and westward directions during deep convection events (Fig 8a). Except on 25 th September and 1-3 October, the meridional wind is showing maximum (~130 m/s to -120 m/s) in the southward direction in comparison with northward wind (Fig 8b). Zonal wind (from Fig 9a) has shown equivalent oscillations both in the eastward and westward direction on almost all the days with a maximum speed of -120 m/s (on 29 th September) in the westward direction. Similar to zonal wind, meridional wind (in Fig 9b) is showing equivalent oscillations in both northward and southward directions on almost all the days with a maximum speed of 120 m/s (on 25 th and 27 th September) in the southward direction. From the Figs 8&9 it is seen that the magnitude of winds are almost invariable with height over MFRTV and are showing increase in magnitude with height over MRTM. Research Paper IJRAR- International Journal of Research and Analytical Reviews 63

[ VOLUME 5 I ISSUE 1 I JAN. MARCH 2018] E ISSN 2348 1269, PRINT ISSN 2349-5138 (a) (b) Fig.10. (a) Height-Time intensity map of zonal (top panel) and meridional (bottom panel) wind fluctuations, for MFRTV location (represented by different colors) during the present observational period, (b) same as (a) but, for MRTM location. Figs 10 & 11 correspond to the height time section of fluctuations of zonal and meridional components over MFRTV and MRTM locations. Meridional wind amplitudes are becoming strong in magnitude when the zonal wind amplitudes are weaker and vice versa, as seen from the fluctuations of the winds from MFRTV. Both zonal and meridional wind amplitudes are showing similar trend except the meridional wind on 26-29 September 2004. In addition to that the zonal wind amplitudes are very weaker in 82-86 km altitude region when compared with meridional wind. Downward phase propagation was 64 IJRAR- International Journal of Research and Analytical Reviews Research Paper

[VOLUME 5 I ISSUE 1 I JAN. MARCH 2018] e ISSN 2348 1269, Print ISSN 2349-5138 http://ijrar.com/ Cosmos Impact Factor 4.236 evidently noticed from the Fig. 10(b). Obviously we can notice the tidal oscillations from the Fig. 10. In order to investigate the periodicities of the tides, the zonal and meridional wind fluctuations have been subjected to wavelet analysis. Figs. 11-14 show the amplitude spectrum of wavelet using zonal and meridional wind fluctuations of MFRTV and MRTM locations. Fig.11. Wavelet amplitude spectrum of zonal wind fluctuation for different height levels obtained using data from MFRTV. Amplitude spectrum of zonal wind (Fig. 11) from MFRTV shows that time-section of significant level corresponding to diurnal tides is decreasing with altitude. Coming to semidiurnal tides the timesection of significant level is invariable with altitude. Significance of terdiurnal tide amplitudes is dominant only from 92-98 km. Fig.12. Same as Fig.9, but for meridional wind fluctuation. Research Paper IJRAR- International Journal of Research and Analytical Reviews 65

[ VOLUME 5 I ISSUE 1 I JAN. MARCH 2018] E ISSN 2348 1269, PRINT ISSN 2349-5138 While that of meridional wind (Fig. 12) from MFRTV shows that time-section of significant level corresponding to diurnal tides is decreasing with altitude, similar to zonal wind. The semidiurnal tides the time-section of significant level is invariable with altitude but there is increase in amplitude with altitude. Significance of terdiurnal tide amplitudes is dominant only from 88-92 km, which is opposite to that of zonal wind in Fig.11. Fig.13. Wavelet amplitude spectrum of zonal wind fluctuation for different height levels obtained using data from MRTM. Amplitude spectrum of zonal wind (Fig. 13) from MRTM shows that time-section of significant level corresponding to diurnal tides is increasing with altitude from 88-98 km (since there is no significant amplitudes at 82 km and 85 km altitudes). Semidiurnal tidal amplitudes show similar significance as that of diurnal tides. Terdiurnal tidal amplitudes show opposite trend as that of diurnal and semidiurnal tides. Fig.14. Same as Fig.11, but for meridional wind fluctuation. 66 IJRAR- International Journal of Research and Analytical Reviews Research Paper

[VOLUME 5 I ISSUE 1 I JAN. MARCH 2018] e ISSN 2348 1269, Print ISSN 2349-5138 http://ijrar.com/ Cosmos Impact Factor 4.236 While the amplitude spectrum of meridional wind (Fig. 14) from MRTM show diurnal, semidiurnal and terdiurnal amplitudes similar to that of zonal wind (Fig.13), but are significantly different with each other. (a) (b) Fig.15. Amplitude (top panel) and phase (bottom panel) profiles of DT, ST, TT and GW (from left to right), respectively. Fig.15 shows height wise variation of amplitude and phase of gravity waves (of ~2-4 hr periodicity and tides. We have subjected the zonal and meridional wind fluctuations for the entire observational period to Fast Fourier Transform (FFT) analysis. The amplitude and phase profiles of diurnal (DT), semidiurnal Research Paper IJRAR- International Journal of Research and Analytical Reviews 67

[ VOLUME 5 I ISSUE 1 I JAN. MARCH 2018] E ISSN 2348 1269, PRINT ISSN 2349-5138 (ST) and terdiurnal (TD) tides along with gravity wave (GW) are shown in Fig.15 (a) & 15 (b) for MFRTV and MRTM respectively. The DT amplitudes are showing increasing trend with height over MFRTV and opposite trend over MRTM. The corresponding phase profiles show almost constant phase with height over MFRTV and varying phase with height over MRTM, with zonal and meridional components showing opposite phase with each other. While that of ST, TT and GW amplitudes of zonal component are showing almost decreasing trend with height where as the meridional component show increasing trend with height over MFRTV. The corresponding phase profiles of zonal and meridional components has shown similar trend with each other. Over MRTM the ST, TT and GW amplitudes of both zonal and meridional components show almost increasing trend with height and their corresponding phase profiles show opposite trend with each other. The phase profiles were observed to be regular for almost all heights over MFRTV. Except for few height levels, the phase difference between the meridional and zonal wind components was 22 hr for DT and 10 hr for ST 1 hr for TT and 0 hr for GW at almost all height levels over MFRTV. But, for MRTM the phase profiles were found to be irregular for almost all heights. The phase profiles were varying between 1 hr and 23 hr for DT, 1 hr and 11 hr for ST, 0 hr and 6 hr for TT and 0 hr and 1 hr for GW over MRTM. Gurbaran et al, [2001] in their study on mesospheric quasi-2 day wave found that the phase profiles were observed to be irregular when the wave amplitudes were smaller for a case and the phase difference between the meridional and zonal wind components was anywhere between 1 hr and 15 hr for another. Even, sometimes they found the difference exceeded 15 hr. In the present study it was similar for MRTM location but, different for MFRTV. 4. Conclusions We have presented the first simultaneous observation of tides associated with intense thunderstorm events followed by the cyclonic event over two equatorial tropical low latitude stations. Dominant diurnal tidal amplitudes with increasing amplitude with height (in the TS region) were noticed by applied wavelet analysis on ECMWF reanalysis data sets of zonal, meridional winds and temperature. Among the amplitude spectrum of these parameters temperature has shown most dominant diurnal tidal amplitude at almost all height levels with 95% significance. But, the amplitude spectrum of zonal and meridional winds show there was strong influence of PWs (of period quasi-2 day or 3 days) on diurnal tides. Even it is true in case of gravity waves, weakening of amplitude in the lower stratospheric region from the amplitude spectrum of vertical velocity that which might be getting modulated by the influence of PWs. It was suggested that the PWs in the mesosphere and ionosphere were the result of upward propagating PWs in the TS region (Forbes and Leveroni, 1992; Smith, 1997). But in the present study the PW amplitudes are restricted to TS region, after that they may be getting refracted to propagate laterally rather than upward, because there is no significant PW amplitudes noticed in MLT region at both the locations. NCEP OLR shows that the convective movement is taking place over and around the MFRTV and MRTM locations with net movement towards the North-East direction. Similarly, the longitudinal motion of tides show that the propagation is towards East and the latitudinal motion confine towards North. Hence, it can be stated that the tides may be propagating vertically up in the north-east ward direction along with the convective movement. Thus, our observations of diurnal tides from the model and observational results may conclude that the deep convective events in the tropical troposphere act as a triggering mechanism for the generation of vertically propagating tidal waves. In the present study it was found that when the amplitudes of zonal and meridional winds show similar trend then their phase profiles show opposite trend and vice versa. Acknowledgements: We sincerely acknowledge Equatorial Geophysical Research Laboratory, Tirunalveli, Space Physical Laboratory,Trivandrum, and Europian Centre for Medium range Weather Forecast for providing necessary data sets for the present study. We are profoundly grateful to Department of Science and Technology for providing fellowship to one of the authors (A.M.S.) and Sri Venkateswara University for providing the necessary laboratory facility to carryout present work. References: 1. Chang, L. C., Ward, W. E., Palo, S. E., Du, J., Wang, D.-Y., Liu, H.-L., Hagan, M. E., et al. 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