Amber is a solid, glass-like material. Amber is formed from a thick, sticky liquid which oozes out of pine trees.

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Fossils give us information about organisms from a long time ago. (a) Amber is a solid, glass-like material. Amber is formed from a thick, sticky liquid which oozes out of pine trees. The image shows two fossil insects in amber. fkienas/istock/thinkstock (i) Suggest how the insects came to be preserved in the amber. (2) Give two other ways fossils are formed.. 2. (2) Page of 22

The fossil record shows that many organisms, including the dinosaurs, became extinct 65 million years ago. One theory was that volcanic activity might have caused this mass extinction. Many scientists believe that this extinction was caused when an asteroid collided with the Earth. (i) A new scientific theory may replace an old theory. Why might this happen? Tick ( ) one box. Evidence from amber is unreliable. Internet evidence is more reliable than fossil evidence. New technology provides more valid evidence. () Give three reasons, other than volcanic activity and collision with an asteroid, why a species may become extinct.. 2. 3. (3) (Total 8 marks) 2 In each question, draw a ring around the correct answer to complete the sentence. (a) Our understanding of how genes are inherited is mostly because of Darwin. the work of Lamarck. Mendel. () Page 2 of 22

A scientist investigated inheritance in pea plants. The scientist crossed tall pea plants with short pea plants. Diagram shows the results. Diagram Some tall offspring Some short offspring In the rest of this question, the following symbols are used to represent alleles. T = allele for tall t = allele for short Page 3 of 22

(i) The st cross in Diagram produced 20 offspring. All of these offspring were tall. TT. This shows that plant contained the alleles Tt. tt. () a dominant allele. Plant 3 is tall because of the environment. a recessive allele. () (c) Diagram 2 gives more information about the cross between plant 3 and plant 4. Diagram 2 This cross produced some tall offspring and some short offspring. :. The ratio of tall to short offspring in Diagram 2 is 2:. 3:. () Page 4 of 22

(d) Two short plants were crossed. This cross produced 00 offspring. 00 short plants. The expected offspring would be 50 tall plants and 50 short plants. 75 tall plants and 25 short plants. () (Total 5 marks) 3 Figure shows a fossil of a sea animal called a Plesiosaur. The Plesiosaur was alive about 35 million years ago. Figure By Andy Dingley (Own work) [CC-BY-SA-3.0 (http://creativecommons.org/licenses/by-sa/3.0)], via Wikimedia Commons (a) How can fossils give evidence for evolution? Tick ( ) one box. Newer fossils are simpler than older fossils. Fossils show change over time. All fossils show the bones of animals. () Page 5 of 22

Plesiosaurs lived in the sea. There was mud at the bottom of the sea. Suggest how the fossil shown in Figure may have been formed after the animal died. (3) (c) Figure 2 shows what scientists think a living Plesiosaur may have looked like. Figure 2 Andreas Meyer/Hemera/Thinkstock Scientists think that the Plesiosaur had smooth skin, with no scales. The scientists cannot be certain what the skin of a Plesiosaur was like. Suggest why. () Page 6 of 22

(d) Plesiosaurs are now extinct. Give two possible reasons why.. 2. (2) (Total 7 marks) 4 Scientists have produced many different types of GM (genetically modified) food crops. (a) Use words from the box to complete the sentence about genetic engineering. clones chromosomes embryos genes GM crops are produced by cutting out of the of one plant and inserting them into the cells of a crop plant. (2) Read the information about GM food crops. Herbicide-resistant GM crops produce higher yields. Scientists are uncertain about how eating GM food affects our health. Insect-resistant GM crops reduce the total use of pesticides. GM crops might breed naturally with wild plants. Seeds for a GM crop can only be bought from one manufacturer. The numbers of bees will fall in areas where GM crops are grown. Use this information to answer these questions. (i) Give two reasons why some farmers are in favour of growing GM crops.. 2. (2) Page 7 of 22

Give two reasons why many people are against the growing of GM crops.. 2. (2) (Total 6 marks) 5 When humans reproduce, chromosomes and genes are passed on to the next generation. In each of the following questions, draw a ring around the correct answer to complete the sentence. cellulose. (a) A gene is a small section of DNA. protein. () X and X. The sex chromosomes in the human male are X and Y. Y and Y. () 23 chromosomes. (c) (i) Most human body cells contain 46 chromosomes. 92 chromosomes. () Page 8 of 22

The number of chromosomes in a human gamete (sex cell) the same number as is half the number in body cells. twice the number () fertilisation. (d) Gametes are produced by meiosis. mitosis. () (Total 5 marks) 6 The photograph shows a fossil footprint. The fossil was found in a rock at the bottom of a shallow river. Scientists believe this is the footprint of a dinosaur. The dinosaur was alive 0 million years ago. Pearl Jackson/iStock (a) (i) Suggest how the fossil shown in the photograph was formed. () Fossils may also be formed by other methods. Describe one other method of forming a fossil. () Page 9 of 22

Dinosaurs are now extinct. Give two factors that can cause extinction.. 2. (2) (c) How can fossils give evidence for evolution? () (d) Scientists are uncertain about how life began on Earth. Why? () (Total 6 marks) 7 In sexual reproduction, an egg fuses with a sperm. (a) (i) Draw a ring around the correct answer to complete the sentence. cloning. An egg and a sperm fuse together in the process of fertilisation. mitosis. () Egg cells and sperm cells each contain the structures given in the box. chromosome gene nucleus List these three structures in size order, starting with the smallest. (smallest) 2 3 (largest) (2) Page 0 of 22

(iii) The egg and the sperm contain genetic material. Draw a ring around the correct answer to complete the sentence. carbohydrate. The genetic material is made of DNA. protein. () The diagram below shows the inheritance of X and Y chromosomes. (i) Draw a tick ( ) on the part of the diagram that shows a sperm cell. () What is the chance of having a female child? Give the reason for your answer. (2) (Total 7 marks) Page of 22

8 (a) Complete the sentences about evolution. Draw a ring around the correct answer to complete each sentence. artificial (i) Darwin suggested the theory of evolution by natural selection. asexual () Darwin s theory of evolution says that all species of living things have artificial evolved from complex life forms. simple three billion () (iii) Most scientists believe that life first developed about three million three thousand years ago. () Darwin s theory of evolution was only slowly accepted by other people. Give two reasons why. 2 (2) Page 2 of 22

(c) Diagram shows one model of the relationship between some animals. Diagram (i) Complete the sentence. The model shown in Diagram is an evolutionary. () Which two of the animals in Diagram are most closely related? and () (iii) Diagram 2 shows a more recent model of the relationship between the animals. Diagram 2 Suggest one reason why scientists have changed the model of the relationships between the animals shown in the diagram. Draw a ring around the correct answer. more powerful computers new evidence from fossils new species discovered () (Total 8 marks) Page 3 of 22

9 This question is about evolution in humans. The graph shows: the estimated brain volume of different species of humans the time when the different species existed on Earth. The data is plotted for modern humans (Homo sapiens) and for three types of extinct ancestors of humans. Millions of years ago Key Each point plotted on the graph shows the estimate for one human. (a) (i) As humans evolved, their brain volume changed. What has happened to human brain volume over the past 4 million years? () Why is the evidence for estimated brain volume for Homo sapiens stronger than the evidence for Australopithecus afarensis? () Page 4 of 22

In a book, the brain volume of a different species, Australopithecus africanus, is stated to be about 600 cm 3. Use evidence from the graphic above to estimate when Australopithecus africanus lived on Earth. Estimate = million years ago () (c) Scientists believe that modern humans evolved by natural selection from Australopithecus afarensis. (i) Complete the following sentence. In the nineteenth century, the scientist who suggested the theory of evolution by natural selection was Charles. () In the nineteenth century, many people did not accept this scientist s theory. Give one reason why. () (Total 5 marks) Page 5 of 22

Mark schemes (a) (i) any two from: trapped / held (since sticky) engulfed / covered by resin allow engulfed / covered by amber prevented decay. 2 any two from: animal / plant (dies and) body covered in sediment / mud ignore ref to rock allow covered in tar / ice bones / shells / hard parts do not decay minerals enter bones / parts are replaced by other materials / mineralisation preserved traces / footprints / burrows / rootlet traces / impressions / casts. 2 (i) New technology provides more valid evidence. any three from: examples of physical factors, e.g. accept 3 physical factors or 3 biological factors or some of each for full marks flooding drought ice age / temperature change. ignore pollution examples of biological factors, e.g. (new) predators (allow hunters) (new) disease / named pathogen competition for food competition for mates competition must be qualified cyclical nature of speciation isolation lack of habitat or habitat change. if no other answers given allow natural disaster / weather change / catastrophic event / environmental change / climate change for mark 3 [8] 2 (a) Mendel Page 6 of 22

(i) TT (c) : a dominant allele (d) 00 short plants [5] 3 (a) fossils show change over time. covered in sediment / mud or sinks into the mud soft parts decay / are eaten or bones / hard parts / shell do not decay minerals enter bones / parts are replaced by minerals / mineralisation accept turns to rock allow is an impression / imprint / cast (c) skin is soft / skin not preserved / not fossilised / skin decays accept not enough / no evidence / no-one has seen one allow this fossil is only bones (d) any two examples of: accept 2 physical factors or 2 biological factors or one of each for full marks physical factors such as volcanic activity (allow volcanoes) / earthquakes / asteroid (collision) / ice age / temperature change ignore pollution and / or biological factors such as predators / disease / named pathogen / competition/ lack of food / mates / cyclical nature of speciation / isolation / lack of habitat or habitat change if no other answers given allow natural disaster / climate change / weather change / catastrophic event / environmental change for mark 2 [7] Page 7 of 22

4 (a) genes chromosomes (i) higher yield less use of pesticides any two from: uncertain about effects on health fewer bees might breed with wild plant seeds only from one manufacturer 2 [6] 5 (a) DNA X and Y (c) (i) 46 chromosomes half the number (d) meiosis [5] 6 (a) (i) animal walking on soft material or suitably named material or further detail eg dries out / buried / hardens / turns to rock do not allow general descriptions of how fossils are formed or reference to bones not decaying Page 8 of 22

any one from: (from) bones / shells / hard parts or from parts that do not decay / rot or are preserved ignore imprint / impression animal trapped in resin / amber / ice / peat allow frozen infiltration with minerals / named any two from: examples of physical factors such as flooding, volcanic activity (allow volcanoes) asteroid collision, drought, ice age / temperature change accept 2 physical factors or 2 biological factors or one of each for full marks ignore pollution examples of biological factors such as predators (allow hunters), disease / named pathogen, competition lack of food / mates, cyclical nature of speciation / isolation / lack of habitat or habitat change If no other answers given allow natural disaster / climate change / weather change / catastrophic event / environmental change for mark 2 (c) older fossils simpler to gain the mark there must be implication of change or (d) change (with time) ignore evolve ignore extinction insufficient / no evidence / no remains or fossils survive ignore no people were there allow no proof [6] 7 (a) (i) fertilisation Page 9 of 22

in sequence: accept next to gene, 2 next to chromosome and 3 next to nucleus in box (iii) gene 2 chromosome 3 nucleus DNA allow mark for smallest or largest in correct position 2 (i) On diagram: tick drawn next to X and / or Y from Parent tick(s) must be totally outside grid squares allow ticks around parent extra ticks elsewhere cancel 0.5 / ½ / 50% / : / 50:50 / in 2 allow 2/4 / 2 in 4 / 2 out of 4 / even(s) / fifty fifty do not allow :2 or 50 / 50 or 50 50 2 (out of 4) boxes are XX or half of the sperm contain an X-chromosome allow XY is male and 2 (out of 4) boxes are XY [7] 8 (a) (i) natural (iii) simple three billion Page 20 of 22

any two from: reference to religion insufficient evidence / couldn t prove it / no proof ignore no evidence mechanism of inheritance / variation not known allow genes / DNA not known about reference to other theories reference to Darwin s status (c) (i) tree 2 hippopotamus and pig both required, either order allow hippo (iii) new evidence from fossils [8] 9 (a) (i) (volume) increases (with time) ignore numbers there is more evidence / specimens / results (for Homo sapiens) allow examples of this, eg more / better fossils allow converse if clearly referring to Australopithecus ignore reference to being more recent 2.5 3.5 (million years ago) accept any number in range (c) (i) Darwin Page 2 of 22

any one from: they believed in other theories allow they believed that God made all life insufficient evidence ignore no evidence no proof allow not enough proof genes / mechanism of inheritance not known / discovered [5] Page 22 of 22