CRDF Grower Bactericide Field Trials

CRDF Grower Bactericide Field Trials Version 1, 4/3/17 Citrus Research & Development Foundation 1

CRDF Grower Bactericide Field Trials Purpose To gather as much data as possible to provide growers with more information on use of Mycoshield, Fireline and Firewall by evaluating grower bactericide programs in simple field trials Variability between trials Any adjuvant, tank mix, timing etc. All regions Any variety Citrus Research & Development Foundation 2

Grower Bactericide Field Trials - Trial Locations CRDF Evaluated Trials Other Evaluator 42 31 Variety Number of Trials Valencia 41 Hamlin 16 Grapefruit 11 Other 6 3

Grower Bactericide Field Trials - Trial Set-up Side-by-side blocks Untreated rows within a treated block Treated Untreated Rows Block 1 Block 2 Treated Block 4

Grower Bactericide Field Trials - Evaluation Methods Disease Severity (Disease Indexing) Divide canopy into quadrants Score each quadrant for visible symptoms on the -5 scale Score the quadrants of both sides of the tree Combine scores for a -4 DI 5

Grower Bactericide Field Trials - Evaluation Methods Disease Severity (Disease Indexing) 1 2 3 4 5 Foliar disease symptoms on <% of the quadrant. A dense quadrant with no twig dieback and minimal blotchy mottle. Foliar disease symptoms on - 4% of the quadrant. Dense quadrant, some twig dieback, some blotchy mottle, and possibly some tufted growth on the canopy. Foliar disease symptoms on 4 6% of the quadrant. Thinning quadrant with noticeable twig dieback and a few areas of open canopy. Blotchy mottle of leaves is common and some tufted growth is apparent. Foliar disease symptoms on 6 8% of the quadrant. Abundantly thin quadrant with obvious twig dieback. The majority of the branches have blotchy mottle and/or tufted growth. Foliar disease symptoms on >8% of the quadrant. The decline has resulted in dieback of large branches and remaining leaves are small, have blotchy mottle and may be deformed 6

Grower Bactericide Field Trials - Evaluation Methods Other Evaluation Methods PCR (copy number or C t ) Fruit Drop Yield For more information see Field Trial Evaluation Methods for Growers on the CRFD website https://app.box.com/s/t961im9c33ue3zzmtp5ju2eym1s8yzoz 7

25.. 15. AB ABC BC ABC CRDF Bactericide Trial One year, 6 yo Hamlin 4 replications, 6 trees per replication 3 applications (spring, summer & fall) AB C AB Disease Severity ABC AB AB AB A AB AB A AB No significant improvement in health AB AB B B 1. 5.. DI Jan16 DI May 16 DI Aug. 16 DI Nov. 16 DI Feb. 17 UTC OTC-1 OTC-2 Streptomycin 8

CRDF Bactericide Trial 7. % Fruit Drop & Yield (kg) A 6. 5. AB AB AB 4. 3. AB A A A. 1.. % Fruit Drop Yield Kg -Foliar OTC1-Foliar OTC2-Foliar Strep-Foliar No significant differences between treatments 9

CRDF Bactericide Trial 35 Fruit Quality 3 25 15 1 5 Lbs Solids Brix/Acid Ratio Color UTC OTC-1 OTC-2 Streptomycin No significant differences between treatments 1

Grower Bactericide Field Trials - Trial Results Variable results Disease severity data shows little separation between treatment and control PCR results extremely variable Some separation between treatment and controls in fruit drop and yield PCR results are reported as copy number, this is a measure of the individual bacterial cells. 11

Untreated 6 5 4 3 1 For these tables, when the p value is.5 or less, then the treatment is significantly different from the untreated control. Grapefruit-1 7. 6. 5. 4. 3.. 1.. Grapefruit-3 Grapefruit-1 N Mean Std Error Mean Std Error DI April16 4 16.7.18 16.8.22 p =.62 DI Sept16 4 11.73.39 11.3.64 p =.3387 DI Oct16 4 9.35.56 1.33.7 p =.1219 DI Nov16 4 11.65.42 11.4.54 p =.5511 DI Jan17 4 13.83.69 14.53.85 p =.7 DI Jan317 4 16.38.49 16.75.37 p =.368 CopyNumberPer1ngDNA_April16 4 433.1 481.51 1176.42 548.39 p =.1518 CopyNumberPer1ngDNA_Jan17 4 42.4 31.19 2.1 123.84 p =.349 FruitDropPercent_Jan17 4 5.83 4.63 4.55 5.1 p =.21 YieldKg_Jan17 4 39.29 4.6 39.15 4.3 p =.972 Grapefruit-3 N Mean Std Error Mean Std Error DI April16 4 16.68.15 16.85.12 p =.3867 DI Sept16 4 9.85.41 8.85.24 p =.259 DI Oct16 4 1..49 9.13.32 p =.573 DI Nov16 4 11.8.32 1.43.24 p =.6 DI Jan17 4 13.98.69 11.98.53 p =.145 DI Jan317 4 16.73.26 16.35.23 p =.2252 CopyNumberPer1ngDNA_April16 4 195.4 157.75 973.99 825.81 p =.358 CopyNumberPer1ngDNA_Jan17 4 113.1 4.27 174.99 72.51 p =.4591 FruitDropPercent_Jan17 4 55.44 3.5 37.56 2.61 p = <.1 YieldKg_Jan17 4 3.9 2.79 44.73 2.59 p =.1 12

Untreated Grapefruit-5 Grapefruit-6 18 16 14 1 1 8 6 4 18 16 14 1 1 8 6 4 Grapefruit-5 N Mean Std Error Mean Std Error DI May16 21.45.34 21..22 p =.2167 DI uly16 18.5.52 18.5.48 p =.5311 DI Aug16 14.3.15 14.95. p =.99 DI Sept16 18.25.39 17.25.48 p =.1151 DI Oct16 19.95.57 17.4.57 p =.9 DI Nov16 19.85.26 18.75.24 p =.18 DI Jan17 23.95.57 23.75.62 p =.7941 CopyNumberPer1ngDNA_May16 12 4575.9 1558.67 2186.56 512.41 p =.3465 CopyNumberPer1ngDNA_Dec16 6928.21 19.74 6394. 154. p =.7151 CopyNumberPer1ngDNA_Jan17 19 2956.55 552.95 3582.58 718.3 p =.4536 FruitDropPercent_Dec16 4.41.37 8.54.67 p = <.1 YieldKg_Dec16 146.53 7.53 86.23 4.92 p = <.1 Grapefruit-6 N Mean Std Error Mean Std Error DI May16.55.23 21..23 p =.1158 DI uly16 16.3.59 17.5.65 p =.396 DI Aug16 14.3.18 14.8.19 p =.632 DI Sept16 16.5.29 16.75.57 p =.6873 DI Oct16 17..41 18..62 p =.1948 DI Nov16.1.32 19.25.42 p =.85 DI Jan17 23.5.53 23.4.52 p =.89 CopyNumberPer1ngDNA_May16 12 2823.71 112.74 169.73 317.4 p =.1346 CopyNumberPer1ngDNA_Dec16 19 4738.94 8.36 7475.39 1113.21 p =.72 CopyNumberPer1ngDNA_Jan17 2856.25 47.5 4662.3 986.11 p =.1283 FruitDropPercent_Dec16 4.37.43 9.47 1. p = <.1 YieldKg_Dec16 155.7 5.79 65.99 4.87 p = <.1 13

Untreated Grapefruit-7 Grapefruit-8 16 14 1 1 8 6 4 35 3 25 15 1 5 Grapefruit-7 N Mean Std Error Mean Std Error HLBDIMax4_May16.6..4. p =.3947 HLBDIMax4_July16 17..49 16..53 p =.2392 HLBDIMax4_Aug16 14.7.19 14.35.29 p =.277 HLBDIMax4_Sept16 15.95.38 16.3.33 p =.4672 HLBDIMax4_Oct16 15.9.32 15.85.55 p =.9243 HLBDIMax4_Nov16 18.95.29 19.1.19 p =.6727 HLBDIMax4_Jan17 24.35.45 23.9.64 p =.5275 CopyNumberPer1ngDNA_May16 13 61.9 1817.66 1575.69 487.96 p =.664 CopyNumberPer1ngDNA_Dec16 16 611.35 824.83 4629.91 561.88 p =.367 CopyNumberPer1ngDNA_Jan17 19 26.36 124.55 99.23 96.87 p =.312 FruitDropPercent_Dec16 4.96.53 5.44.37 p =.475 YieldKg_Dec16 128.2 9.8 128.73 6.76 p =.9466 Grapefruit-8 N Mean Std Error Mean Std Error HLBDIMax4_May16.3.22.6.22 p =.3112 HLBDIMax4_July16 16.7.56 17..55 p =.6715 HLBDIMax4_Aug16 14.25.23 14.35.24 p =.729 HLBDIMax4_Sept16 16.1.3 16.75.38 p =.864 HLBDIMax4_Oct16 16.25.54 16..53 p =.9457 HLBDIMax4_Nov16 18.95.3 18.8.26 p =.7164 HLBDIMax4_Jan17 25.55.6 24.85.49 p =.3863 CopyNumberPer1ngDNA_May16 79.93 43.91 838.44 325.64 p =.124 CopyNumberPer1ngDNA_Dec16 17 431.61 973.72 5375.32 966.3 p =.3496 CopyNumberPer1ngDNA_Jan17 19 3431.34 679.33 59. 43.17 p =.852 FruitDropDec_16 22. 2.32 28. 3.11 p =.97 14

Untreated Hamlin-1 Hamlin-2 3 25 25 15 1 5 15 1 5 Hamlin-1 N Mean Std Error Mean Std Error DI March16 21.6.62 21.5.39 p =.8884 DI Sept16 16.55.28 16.55.28 p = 1 DI Oct516 18.7.45 18.35.6 p =.6261 DI an17 19.2.39 18.9.44 p =.6168 CopyNumberPer1ngDNA_March16 19 329152.9 44554.16 255979.39 34197.44 p =.54 CopyNumberPer1ngDNA_Jan17 17 5896.73 1257.5 6683.32 1764.42 p =.7158 FruitDropJan17 253.8 17.65 213.55 11.36 p =.557 Yield (Boxes/Acre) 215 Hamlin-2 N Mean Std Error Mean Std Error DI March16 21.15.47.95.22 p =.655 DI Sept16 17.. 16.5.25 p =.341 DI Oct516 16.9.29 18.5.34 p =.6 DI an17 19..33.95.32 p =.3 CopyNumberPer1ngDNA_March16 335638.83 51686.22 348813.63 36218.84 p =.976 CopyNumberPer1ngDNA_Jan17 18 765.45 2144.31 4683.1 135.37 p =.316 FruitDropJan_17 193.9 7.17 151.8 11.28 p =.32 Yield (Boxes/Acre) 215 268 15

Untreated 5 45 4 35 3 25 15 1 5 Hamlin-3 16 14 1 1 8 6 4 Hamlin-4 Hamlin-4 Hamlin-3 N Mean Std Error Mean Std Error DI April16 19.9.46.8.47 p =.661 DI Sept16 12.7.42 13.9.58 p =.32 DI Oct16 16.8.24 17.5.45 p =.1387 DI Nov16 17.15.32 16.95.38 p =.6191 DI Feb16 15.4.39 15.6.65 p =.7168 CopyNumberPer1ngDNA_April16 26.28 1859.36 675.24 2865.56 p =.2 CopyNumberPer1ngDNA_Dec16 5132.25 1218.27 3886.71 1652.78 p =.4998 FruitDropPercent_Dec16 18.58 2.23 19.67 2.12 p =.6334 YieldKg_Dec16 4.87 3.75 36.72 3.7 p =.1891 N Mean Std Error Mean Std Error DI May16 24.65.18 25..21 p =.683 DI Sept16.5.28 22.65.6 p =.4 DI Oct516 17.15.5 18.55.61 p =.834 DI Oct1916 18.7.21 21.5.47 p = <.1 DI Octave16 17.93.32 19.8.45 p =.13 DI Nov16 26.9 1.37 33.15 3.77 p =.68 DI an17 22.15.58 26..45 p = <.1 CopyNumberPer1ngDNA_May16 14 4771.43 1352.58 1158.36 2997.51 p =.1782 CopyNumberPer1ngDNA_Dec16 18 3519.67 721.35 559.1 131.67 p =.1598 FruitDropPercent_Dec16 12.56.93 18.92 2.4 p =.33 YieldKg_Dec16 142.53 7.7 117.8 8.58 p =.48 16

Untreated Hamlin-5 Hamlin-6 1 9 8 7 6 5 4 3 1 18 16 14 1 1 8 6 4 Hamlin-5 N Mean Std Error Mean Std Error DI May16 14.4.6 12.5.15 p = <.1 DI Sept16 14.55.32 14.7.35 p =.768 DI Oct16 15.55.21 16.5.29 p =.82 DI an17 15.85.75 18.35.52 p =.22 CopyNumberPer1ngDNA_May16 27.69 26.86 14.91 11.48 p =.6655 CopyNumberPer1ngDNA_Jan17 1349.41 8475.73 3477.97 1246.53 p =.4531 FruitDropPercent_Jan17 44.4 1.9 76.95 1.3 p =.137 Hamlin-6 N Mean Std Error Mean Std Error DI May16. 16.5.15 17.5.11 p = <.1 DI Sept16. 15.15.34 15.95.47 p =.849 DI Oct16. 16.75.4 16.6.24 p =.762 DI Jan17. 16.35.43 14.9.77 p =.79 CopyNumberPer1ngDNA_May16..3.3.. p =.3248 CopyNumberPer1ngDNA_Jan17. 117.48 117.48 46786.3 448676.36 p =.36 FruitDropPercent_Jan17. 5.1.56 6.7.94 p =.368 YieldKg_Jan17. 157.33 12.97 139.77 1.26 p =.294 17

Untreated Hamlin-7 Hamlin-8 45 4 35 3 25 15 1 5 6 5 4 3 1 Hamlin-7 N Mean Std Error Mean Std Error DI May16 16.85.17 16.7.16 p =.527 DI Sept16 15.15.4 14.5.39 p =.2421 DI Oct516 16.95.34 16.35.18 p =.373 DI Jan17 16.95.27 16.5.22 p =.147 CopyNumberPer1ngDNA_May16 19 844.17 2927.85 946.86 66.98 p =.212 CopyNumberPer1ngDNA_Jan17 19 3924.57 742.54 2968.79 51.74 p =.2545 FruitDropPercent_Jan17 34.7 3.23 37.67 3.27 p =.549 YieldKg_Jan17 31.485 2.8 28.26 3.35 p =.4663 Hamlin-8 N Mean Std Error Mean Std Error DI May16 16.65.17 17.45. p =.2 DI Sept16 14.35.41 16.95.41 p = <.1 DI Oct516 17.55.22 18.5. p =.38 DI Dec516 17.9.25 18.65.26 p =.596 CopyNumberPer1ngDNA_May16 148.93 18.91 2371.41 121.57 p =.823 CopyNumberPer1ngDNA_Dec16 18 2464.3 685.73 646.74 129.92 p =.4 FruitDropPercent_Dec16 26.5 3.77 48.72 4.51 p = <.1 YieldKg_Dec16 43.53 4.39 29.4 3.96 p =.72 18

Untreated Hamlin-9 Hamlin-1 6. 3. 5. 4. 3.. 1.. 25.. 15. 1. 5.. DI Baseline DI Oct DI March PCR Baseline PCR Jan 17 Fruit Drop Yield Ratio s Hamlin-9 N Mean Std Error Mean Std Error DI June16 22.9.72 23.85.22 p =.8 DI Sept16 16.1 2.34 14.65.33 p =.169 DI Nov16 19.1 3.14 19..6 p =.8818 DI _Jan17 21. 5.42.65.64 p =.5623 CopyNumberPer1ngDNA_June16 14732.63 11283.84 12367.33 2335.99 p =.5145 CopyNumberPer1ngDNA_Dec16 637.77 3412.64 6455.81 739.12 p =.894 FruitDropPercent_Dec16 16.58 12.45 11.82 1.44 p =.125 YieldKg_Dec16 37.41 11.51 47.22 2.51 p =.114 Hamlin-1 s DI Baseline DI Oct DI March PCR Baseline PCR Jan 17 Fruit Drop Yield Ratio N Mean Std Error Mean Std Error 25 23..26 23.4.34 p =.9287 25 22.32.26 22.4.3 p =.8491 25 23.44.29 25.88.69 p =.28 25 38.79.79 29.55 1.91 25 33.19 1.61 28.34 1.56 p = <.1 p =.26 25 12.84 4.29 82.48 4.27 p =.12 25 248.92 8.65 226. 11.48 p =.18 25 19.58.15 19.4.13 p =.6999 19

Untreated 35 Hamlin-11 Midsweet-1 3 18 16 25 14 1 1 8 15 6 4 1 5 DI Baseline DI Oct DI March PCR Baseline PCR Jan 17 Fruit Drop Yield s Midsweet-1 N Mean Std Error Mean Std Error s Hamlin-11 Mean Std Error Mean Std Error DI Baseline 22.64.36 22.16.36 p=.381 DI Oct 23.48.33 25.32.49 p=.2 DI March 21.96.61 23.72 1.2 p=.113 PCR Baseline 25.52 1.48 21.94 1.14 p=.66 PCR Jan 17 24.53.78 23.93.29 p=.499 Fruit Drop 188.12 1.55 185.64 11.95 p=.882 Yield 277.86 15.14 221.66 15.74 p=.9 Ratio 13..23 12.47.17 p=.28 HLBDIMax4_March16 25 19.16.46 18.84.43 p =.6132 HLBDIMax4_Oct516 25 17.64.41 17.48.34 p =.7636 HLBDIMax4_Nov516 25 23.12.29 22.72.23 p =.2846 HLBDIMax4_Dec16 25 4.84 3.26 39.12 3.65 p =.7251 HLBDIMax4_Jan17 25 21.4.68 28.36 7.26 p =.355 CopyNumberPer1ngDNA_March16 25 1311.96 1311.96 641.32 61197.12 p =.3274 CopyNumberPer1ngDNA_Jan17 22 2488.88 85.45 4777.7 1494.15 p =.2742 FruitDropJan_17 25 173.28 1.84 169.22 1.47 p =.7737

Untreated 18 16 14 1 1 8 6 4 Midsweet-2 1. 1. 8. 6. 4... Midsweet-3 Midsweet-2 N Mean Std Error Mean Std Error DI April16 22.45.64 21..46 p =.97 DI Oct16 19.6.94.5.73 p =.1825 DI Nov16 21.95.54.7.81 p =.769 CopyNumberPer1ngDNA_April16 8482.9 3627.43 1561.81 1235.95 p =.358 CopyNumberPer1ngDNA_Jan17 1249.95 41.61 711.47 314.67 p =.2142 FruitDropPercent_Feb17 15.75 2.51 13.85 3.34 p =.6183 YieldKg_Feb17 19.19 15.41 141.83 17.49 p =.915 Midsweet-3 N Mean Std Error Mean Std Error DI April16.9.35 21.45.38 p =.1884 DI October16 16.85.8 18.65.63 p =.265 DI Nov16 19.4.35.7.46 p =.92 DI anuary17 15.6.62 16.75.8 p =.1279 CopyNumberPer1ngDNA_April16 94.37 32.16 1123.63 96.38 p =.289 CopyNumberPer1ngDNA_Jan16 27.61 878.74 2472.68 1167.84 p =.733 FruitDropPercent 8.4 1.66 13.66 1.49 p =.61 YieldKg_Jan17 98.23 6.54 93.71 9.29 p =.61 21

Untreated Parson Brown-1 Parson Brown-2 25 25 15 15 1 1 5 5 DI Baseline DI Oct DI March PCR Baseline PCR Jan 17 Fruit Drop Yield Ratio DI Baseline DI Oct DI March PCR Baseline PCR Jan 17 Fruit Drop Yield Ratio s s Parson Brown-1 s Mean Std Error Mean Std Error DI Baseline 22.96.38 22.44.33 p=.357 DI Oct 23.6.31 24..28 p=.144 DI March 28.6.75 29.64.67 p=.3274 PCR Baseline.2.22 21.14.33 p=.124 PCR Jan 17 23.57.15 22.47.48 p=.281 Fruit Drop 123.68 11.2 128.68 15.32 p=.7996 Yield 187.28 13.4 198.77 18.1 p=.647 Ratio.1.12 19.18.36 p=.3146 Parson Brown-2 s Mean Std Error Mean Std Error DI Baseline 24.4.21 22.76.38 p=.4 DI Oct 21.8.25 21.76.36 p=.122 DI March 23.88.4 21.84.39 p= <.1 PCR Baseline 21.33 1.8 23.14 1.43 p=.312 PCR Jan 17.89 1..77.72 p=.919 Fruit Drop 29.64 3.19 31.4 2.15 p=.6182 Yield 188.98 1.15 191.4 13.99 p=.8926 Ratio 23.65.44 23.48.37 p=.8982 22

Untreated Valencia-1 Valencia-11 8. 7. 6. 5. 4. 3.. 1.. 6 5 4 3 1 Valencia-1 N Mean Std Error Mean Std Error DI May16 18.8.16 18.9.16 p =.6727 DI Oct16 14.9.43 13.7.42 p =.19 DI Jan17 14.4.24 13.8.28 p =.159 CopyNumberPer1ngDNA_May16 19571.2 444.41 28182.7 4421.76 p =.1492 CopyNumberPer1ngDNA_Jan17 2254.8 441.41 1214.87 3.99 p =.488 FruitDropPercent_Feb17 17.52 1.38 13.76 1.4 p =.171 YieldKg_Feb17 56. 2.95 65.77 2.62 p =.71 Valencia-11 N Mean Std Error Mean Std Error DI May16 19.5.18 18.9.18 p =.5487 DI Oct16 15.85.31 15.3.4 p =.1852 DI Jan17 14.6.36 15.5.44 p =.82 CopyNumberPer1ngDNA_May16 2697.33 315.29 24225.89 3624.43 p =.5851 CopyNumberPer1ngDNA_Jan17 16 9184.56 2198.74 1186.31 331.15 p =.9 FruitDropPercent_Feb17 15.18.87 15.1 1.27 p =.914 YieldKg_Feb17 51.28 2.7 53.25 2.4 p =.4498 23

Bactericideand Timing March-May June-Aug. Sept.-Nov. Trial Site RS Age Average DI Location FW FL MS FW FL MS FW FL MS Grapefruit-1 Sour <5 16 IR 2 2 Grapefruit-3 Sour <5 16 IR 2 2 1 Grapefruit-5 Swingle + 21 IR 2 2 Grapefruit-6 Swingle + 21 IR 2 2 Grapefruit-7 Sour + 21 IR 2 2 Grapefruit-8 Sour + IR 2 2 Hamlin-1 Swingle + 21 R 2 1 1 Hamlin-2 Swingle + 19 R 2 1 1 Hamlin-3 Carrizo <5 SW 1 1 Hamlin-4 Sour + 25 IR 1 2 1 1 Hamlin-5 Swingle <5 13 SW 2 2 1 Hamlin-6 F 8 + 17 SW 1 1 1 1 Hamlin-7 Carrizo 5-1 17 SW 2 2 Hamlin-8 Swingle <5 17 SW 1 1 1 Hamlin-9 5-1 23 R Hamlin-1 Swingle + 23 SW 1 1 1 1 Hamlin-11 Carrizo + 23 SW 1 1 1 1 Parson Brown-1 Cleo + 24 SW 1 1 1 1 Parson Brown-2 Carrizo + 23 SW 1 1 1 Midsweet-1 Swingle 5-1 22 R 2 1 1 Midsweet-2 Carrizo 5-1 22 SW 1 1 Midsweet-3 Swingle 5-1 21 SW 1 1 Valencia-1 Swingle 5-1 19 IR 1 2 1 1 Valencia-11 Swingle 5-1 19 IR 1 2 1 1 FW = Firewall FL = Fireline MS = Mycoshield 24

Untreated The PCR results for the following trials are reported as Ct values of 1-4. The higher the number, the less bacteria in the sample. Hamlin-12 Hamlin-13 35. 4 3. 35 25. 3 25. 15. 15 1. 1 5. 5. DI Spring DI Winter Ct Spring Ct Winter DI Spring DI Winter Ct Spring Ct Winter Hamlin-12 Mean Std Error Mean Std Error DI Spring 23.9.54 22.8.62 p =.15 DI Winter 25..38 25.45.38 p =.39 Ct Spring 26.36.81 26.62.9 p =.83 Ct Winter 28.27 1.3 27.8 1.9 p =.77 Yield 1.82 1.93 Hamlin-13 Mean Std Error Mean Std Error DI Spring 27.85 1.39 28..36 p =.91 DI Winter 26.85.59 28.95.32 p =. Ct Spring 23.91.4 25.73.51 p =.1 Ct Winter 29.1 1.45 33.47 1.57 p =.2 Yield 1.85 2.17 25

Untreated Hamlin-14 Hamlin-16 35 35. 3 3. 25 25.. 15 15. 1 1. 5 5. DI Spring DI Winter Ct Spring Ct Winter. DI Spring DI Winter Ct Spring Ct Winter Hamlin-14 Mean Std Error Mean Std Error DI Spring 26.25.43 25.85.33 p =.6 DI Winter 21.7.79 23.25.82 p =.89 Ct Spring 25.97.58 26.65.42 p =.26 Ct Winter 29.55 1.8 29.88 1.11 p =.47 Yield 1.67 1.49 Hamlin-16 Mean Std Error Mean Std Error DI Spring 27.9.38 3.15.33 p = <.1 DI Winter 27.85.26 28..28 p =.21 Ct Spring 26.2.41 26.38.43 p =.53 Ct Winter 25.89.84 29.37 1.26 p =.3 Yield 1.91 1.92 26

Untreated Hamlin-17 Midsweet-4 35 32 3 31 25 3 29 28 15 27 1 26 25 5 24 DI Spring DI Winter Ct Spring Ct Winter 23 DI Spring DI Winter Ct Spring Ct Winter Hamlin-17 Mean Std Error Mean Std Error DI Spring 25.5.39 26.25.28 p =.12 DI Winter 27..54 27.3.65 p =.9 Ct Spring 3.8 1.52 27.88 1.9 p =.21 Ct Winter 27.33 1.26 27.8 1.43 p =.82 Yield 1.28 1.47 Midsweet-4 Mean Std Error Mean Std Error DI Spring 3.15.33 3.35. p =.615 DI Winter 3.4.33 28.75.23 p =.3 Ct Spring 26..39 27.38.34 p =.5532 Ct Winter 28.37.96 27.64.89 p =.5165 Yield 1.47 1.63 27

Untreated 4. Valencia-28 35. Valencia-31 35. 3. 3. 25. 25... 15. 15. 1. 1. 5. 5.. DI Spring DI Winter Ct Spring Ct Winter. DI Spring DI Winter Ct Spring Ct Winter Valencia-28 Mean Std Error Mean Std Error DI Spring 25.76.26 26.12.24 p =.23 DI Winter.6.71 23.88.8 p =. Ct Spring 25.88.99 27.36 1.48 p =.37 Ct Winter 32.14 1.42 25.92 1.5 p = <.1 Valencia-31 Mean Std Error Mean Std Error DI Spring 25.5.31 25.85.25 p =.28 DI Winter 25.45.23 24.8.19 p =.2 Ct Spring 25.44.29 27.76.5 p = <.1 Ct Winter 25.26.44 26.96.61 p =.2 28

Untreated Valencia-32 Valencia-33 29 3 28 29 27 28 26 27 25 26 24 25 23 24 22 DI Spring DI Winter Ct Spring Ct Winter 23 DI Spring DI Winter Ct Spring Ct Winter Valencia-32 Mean Std Error Mean Std Error DI Spring 26.5.25 25.95.32 p =.834 DI Winter 25.95.34 26.65.38 p =.1627 Ct Sping 26.31.78 27.25 1.19 p =.518 Ct Winter 25.3.43 25.11.33 p =.7274 Valencia-33 Mean Std Error Mean Std Error DI Spring 25.8.35 25.7.27 p =.83 DI Winter 26.1.3 26.15.33 p =.9 Ct Spring.. p = Ct Winter 28.73.69 26.12.5 p =. Yield 2.56 2.48 29

Untreated 35 Valencia-34 29. 28. Valencia-35 3 27. 25 26. 25. 15 24. 1 23. 5 22. DI Spring DI Winter Ct Spring Ct Winter 21. DI Spring DI Winter Ct Spring Ct Winter Valencia-34 Mean Std Error Mean Std Error DI Spring 28.75.25 28.7.4 p =.963 DI Winter 28.95.35 29.7.62 p =.315 Ct Spring 3. 1.24 26.75.47 p =.4142 Ct Winter 25.32.91 26.41.92 p =.3991 Valencia-35 Mean Std Error Mean Std Error DI Spring 26.35.31 26.1.24 p =.53 DI Winter 26..33 27.4.61 p =.3 Ct Spring 25.28.39 27.4 1.9 p =.14 Ct Winter 24.8.57 24.46.75 p =.65 3

Untreated 35. Valencia-37 45. 4. Valencia-38 3. 35. 25.. 3. 25.. 15. 15. 1. 1. 5. 5.. DI Spring DI Winter Ct Spring Ct Winter. DI Spring DI Winter Ct Spring Ct Winter Valencia-37 Mean Std Error Mean Std Error DI Spring 23.55.42 23.65.64 p =.9 DI Winter 22..56 24.15.85 p =.3 Ct Spring 25.13.38 26.11.99 p =.38 Ct Winter 26.83.73 27.99 1.17 p =.24 Yield 1.97 1.59 Valencia-38 Mean Std Error Mean Std Error DI Spring 16.85.7 16.65.89 p =.8313 DI Winter 22..62 25.65.62 p = <.1 Ct Spring 36.88 1.31 33.6 1.71 p =.1599 Ct Winter 35.56 1.7 34.42 1.71 p =.441 31

Untreated Valencia-4 Grapefruit-9 4. 45 35. 4 3. 35 25. 3 25. 15. 15 1. 1 5. 5. DI Spring DI Winter Ct Spring Ct Winter DI Spring DI Winter Ct Spring Ct Winter Valencia-4 Mean Std Error Mean Std Error DI Spring.95.53 21..67 p =.9519 DI Winter 22.15.6 25.95.8 p =.4 Ct Spring 28.85 1.19 34.14 1.32 p =.4 Ct Winter 23.79.43 27.58 1.7 p =.33 Grapefruit-9 Mean Std Error Mean Std Error DI Spring 26.55.37 26.75.32 p =.6428 DI Winter 29.4.48 28.4.77 p =.1711 Ct Spring 37.76 1.6 36.24 1.33 p =.2538 Ct Winter 35.8 1.25 36.26 1.48 p =.83 32

Untreated 4 Pineapple-1 5. Murcott-1 35 45. 3 4. 25 35. 3. 25. 15. 1 15. 1. 5 5. DI Spring DI Winter Ct Spring Ct Winter. DI Spring DI Winter Ct Spring Ct Winter Pineapple-1 Mean Std Error Mean Std Error DI Spring 26.55.34 27.35.39 p =.1283 DI Winter 28.95.41 28.75.43 p =.7244 Ct Spring 33. 1.48. p =.9619 Ct Winter 25.45 1.3 25.37 1.11 p = <.1 Yield 1 1 Murcott-1 Mean Std Error Mean Std Error DI Spring 25.3.79 27.95.79 p =.2 DI Winter 32.8.73 35.95.26 p =. Ct Spring 39.86.14 na. p = <.1 Ct Winter 29.6 1.72. 6.92 p =.5 Yield 1.39 1.29 33

Valencia-3 Valencia-29 25 4 35 3 15 25 1 15 5 1 5 DI Spring DI Winter Total # Fruit Yield (kg) DI Spring DI Winter Total # Fruit Yield (kg) Valencia-3 Mean Std ErrorMean Std Error DI Spring 25.6 27.8 p =.1 28.5 31.7 DI Winter p = <. 1 PCR Spring 25.5 26.6 p =.16 PCR Winter 26.5 27.9 p =.25 Total # Fruit 229.33 218.36 p =.45 Yield (kg) 36.14 33.15 p =.25 Valencia-29 Mean Std ErrorMean Std Error DI Spring 25.5 25.6 p =.42 DI Winter 27.5 26.6 p =.5 PCR Spring 26.5 26.5 p =.25 PCR Winter 27.11 26.7 p =.55 Total # Fruit 367.43 339.43 p =.24 Yield (kg) 56.17 58.19 p =.65 34

9 Valencia-15 1 Valencia-16 8 9 7 8 6 5 7 6 5 4 4 3 3 1 DI May16 DI Oct16 DI Jan17 % Fruit Drop Yield (kg) Valencia-15 Mean Std ErrorMean Std Error DI May16 23.4 23.4 p =.83 DI Oct16 18.6 18.6 p =.77 DI Jan17 16.7 17.7 p =.81 PCR May16 77174 12.77 118 15.34 p =.18 PCR Jan17 4175 2.97 3777 2.78 p =.71 % Fruit Drop 13.14 15.13 p =.26 Yield (kg) 8.21 78.21 p =.84 1 DI May16 DI Oct16 DI Jan17 % Fruit Drop Yield (kg) Valencia-16 Mean Std ErrorMean Std Error DI May16 24.1 24.1 p =.8 DI Oct16 19.1.1 p =.37 DI Jan17 18.1 18.1 p =.7 PCR May16 4389 2.8 71624 2.76 p =.17 PCR Jan17 2746.51 2264.48 p =.54 p = <. % Fruit Drop 21.2 13.2 1 Yield (kg) 83.3 86.3 p =.35 35

Valencia-38 Valencia-7 14 9 1 1 8 6 4 DI Spring DI Winter Total # Fruit Yield (kg) Valencia-38 Mean Std ErrorMean Std Err DI Spring 17.9 17.1 p =.86 DI Winter 22.8 26.8 p = <.1 PCR Spring 37.12 34.14 p =.14 PCR Winter 36.14 34.14 p =.65 Total # Fruit 1217 1.8 142 1.1 p =.25 Yield (kg) 185.38 145.37 p =.3 8 7 6 5 4 3 1 DI April16 DI Oct16 DI Jan17 % Fruit Drop Yield (kg) Valencia-7 Mean Std ErrorMean Std Err DI April16 22.5 22.5 p =.8 DI Oct16 14.5 15.6 p = <.1 DI Jan17 17.5 17.7 p =.15 PCR April16 87896 13.78 4129 9.32 p =.2 PCR Jan17 2697 3.86 217 2.11 p =.71 % Fruit Drop 23.19 22.14 p =.81 Yield (kg) 78. 77.18 p =.83 36

Valencia-28 4 35 3 25 15 1 5 DI Spring DI Winter Total # Fruit Yield (kg) Valencia-28 Mean Std ErrorMean Std Error DI Spring 26.4 26.4 p =.27 DI Winter 21.7 24.8 p =.1 PCR Spring 26.8 27.1 p =.35 32.1.9 PCR Winter 26 p = <. 1 Total # Fruit 371.4 355.42 p =.59 Yield (kg) 76. 68.19 p =.22 37

Bactericides and Timing March-May June-Aug. Sept.-Nov. Trial Site Region Rootstock Age Average DI at Start FW FL MS FW FL MS FW FL MS Grapefruit -1 IR Sour <5 16 2 2 1 Grapefruit -2 IR Sour <5 16 2 2 1 Grapefruit -3 IR Sour <5 16 2 2 1 Grapefruit -4 IR Sour <5 16 2 2 1 Grapefruit -5 IR Swingle + 21 2 2 Grapefruit -6 IR Swingle + 21 2 2 Grapefruit -7 IR Sour Orange + 2 2 Grapefruit -8 IR Sour Orange + 2 2 Grapefruit-9 1-27 1 1 1 1 1 FW = Firewall FL = Fireline MS = Mycoshield 38

Bactericides and Timing March-May June-Aug. Sept.-Nov. Trial Site Region Rootstock Age Average DI at Start FW FL MS FW FL MS FW FL MS Hamlin-1 R Swingle + 21 2 1 1 Hamlin-2 R Swingle + 19 2 1 1 Hamlin-3 SW Carrizo <5 1 1 Hamlin-4 IR sour + 29 1 2 1 1 Hamlin-5 SW Swingle <5 13 2 2 1 Hamlin-6 SW F 8 + 17 1 1 1 1 Hamlin-7 SW Carrizo 5-1 17 2 2 Hamlin-8 SW Swingle <5 17 1 1 1 Hamlin-9 R 5-1 23 Hamlin-1 SW Swingle + 1 1 1 1 Hamlin-11 PR Carrizo + Hamlin-12 R 5-1 23 1 1 1 1 1 Hamlin-13 R 5-1 28 1 1 1 1 1 Hamlin-14 R 5-1 26 1 1 1 1 1 Hamlin-15 R 5-1 25 1 1 1 1 1 1 Hamlin-16 SW 5-1 29 2 1 1 1 1 Hamlin-17 R 5-1 26 1 1 1 1 1 1 FW = Firewall FL = Fireline MS = Mycoshield 39

Bactericides and Timing March-May June-Aug. Sept.-Nov. Trial Site Region Rootstock Age Average DI at Start FW FL MS FW FL MS FW FL MS Valencia-1 R + 21 1 1 Valencia-2 R Swingle + 21 1 1 Valencia-3 SW Carrizo <5.6 1 1 Valencia-4 SW Kuharske 5-1 25 1 1 1 1 1 1 Valencia-5 SW Kuharske 5-1 24 1 1 1 1 1 1 Valencia-6 SW Kuharske 1-25 1 1 1 1 1 Valencia-7 IR Swingle 5-1 22 1 1 1 1 1 Valencia-8 IR Swingle 5-1 23 1 1 1 1 1 Valencia-9 IR Swingle 5-1 23 1 1 1 1 1 Valencia-1 IR Swingle 5-1 19 1 2 1 1 Valencia-11 IR Swingle 5-1 19 1 2 1 1 Valencia-12 SW Carrizo + 25 1 1 1 1 Valencia-13 SW Carrizo + 21 1 1 1 1 Valencia-14 SW Carrizo + 25 1 1 1 1 Valencia-15 R Swingle 1-23 1 1 1 1 1 Valencia-16 R Swingle 1-24 1 1 1 1 1 Valencia-17 R Swingle 1-1 2 1 Valencia-18 R Swingle 1-1 2 1 Valencia-19 R Swingle 1-1 2 1 Valencia- R Swingle 1-1 2 1 FW = Firewall FL = Fireline MS = Mycoshield 4

Bactericides and Timing March-May June-Aug. Sept.-Nov. Trial Site Region Rootstock Age Average DI at Start FW FL MS FW FL MS FW FL MS Valencia-21 SW Carrizo + 22 1 1 Valencia-22 SW Carrizo + 25 1 1 1 1 Valencia-23 SW Swingle 5-1 24 2 2 1 Valencia-24 PR Swingle + 1 1 1 Valencia-25 R Volk-Carrizo + Valencia-26 SW Carrizo + 1 1 2 Valencia-27 SW Carrizo + Valencia-28 R 5-1 26 1 1 1 1 1 Valencia-29 R 5-1 25 1 1 1 1 1 1 Valencia-3 R 5-1 26 1 1 1 1 1 Valencia-31 SW 5-1 26 1 1 1 1 1 Valencia-32 SW 1-26 1 1 1 1 1 Valencia-33 R 1-26 1 1 1 1 1 1 Valencia-34 R 1-29 1 1 1 1 1 Valencia-35 R 1-26 1 1 1 1 1 Valencia-36 PR 5-1 28 1 2 1 Valencia-37 R 5-1 31 1 1 1 1 1 1 Valencia-38 R + 17 1 1 1 1 Valencia-39 R 1-27 1 1 1 1 1 Valencia-4 R 5-1 21 1 1 1 1 1 FW = Firewall FL = Fireline MS = Mycoshield 41

Bactericides and Timing March-May June-Aug. Sept.-Nov. Trial Site Region Rootstock Age Average DI at Start FW FL MS FW FL MS FW FL MS Midsweet-1 R Swingle 5-1 22 2 1 1 Midsweet-2 SW Carrizo 5-1 22 1 1 Midsweet-3 SW Swingle 5-1 21 1 1 Midsweet-4 SW 5-1 3 1 1 1 1 1 1 Murcott-1 R 1-27 1 1 1 Parson-1 PR Cleo + 1 1 1 1 Parson-1 PR Cleo + Parson-2 SW Carrizo + 1 1 1 Parson-2 SW Cleo + Pineapple-1 R 1-27 1 1 1 1 FW = Firewall FL = Fireline MS = Mycoshield 42