Pepper cultivation on a substrate consisting of soil, natural zeolite and olive mill waste sludge-changes in soil properties

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Pepper cultivation on a substrate consisting of soil, natural zeolite and olive mill waste sludge-changes in soil properties S. Kosmidis 1, M. K. Doula 2, A. Assimakopoulou 1, Ch. Kolovos 2, Y. Troyanos 2, A. Papadopoulos 2, P. Kostopoulos 3 1 TEI of Peloponnese, Department of Agricultural Technology 2 Benaki Phytopathological Institute 3 Agricultural University of Athens, Naxos, June 2018

Characteristics: Seasonal production (from November to February) High water content High organic load High content in polyphenols High electrical conductivity High content of nutrients (potassium, iron) High oil content Olive Mill Waste Management of olive mill wastes: Disposal in evaporation ponds Disposal on soils and on water recipients Treatment in special installations (production of soil improvers, use in the irrigation of the treated liquid part) Treatment and retrieval of phenols

OMW : Uncontrolled disposal on soils LIFE PROSODOL ph Electrical Conductivity Organic Matter Total Nitrogen Available Phosphorus Exchangeable Potassium Polyphenols Available iron Olive wastes on soil Electrical Conductivity up to 10 ms/cm Exchangeable Potassium up to 20 cmol/kg Available Phosphorus up to 440 ppm Available Iron up to 380 ppm Total Nitrogen up to 20 mg/g Polyphenols up to 600 ppm

Solid Olive Mill Waste 3 doses 0%, 2.5%, 5.0% 12 weeks greenhouse experiment Pepper plants in 36 pots 3 doses of zeolite (clinoptilolite) 0%, 2.5%, 5.0% 0% ZEOLITE 2.5% ZEOLITE 5.0% ZEOLITE Z0AP0 Z1AP0 Z2AP0 Z0AP1 Z1AP1 Z2AP1 Z0AP2 Z1AP2 Z2AP2 The pepper seedlings were planted in 9 different soil/solid olive mill waste/zeolite mixtures. Εach kind of mixture was placed in 4 pots.the experimental design was based on a split plot pattern. Plants were irrigated every other day by adding 1000ml of water, while leachates, were collected and analyzed weekly. Peppers and pot soils were harvested and collected respectively, for further research and conclusions. Naxos, June 2018

Table 1: OMW properties Parameters 12 weeks greenhouse experiment Value Organic Matter, % 97 Electrical 2,6 Conductivity, ms/cm ph 4,10 Polyphenols, % 0,32 Nitrogen, % 0,51 Potassium, % 1,1 Calcium, % 0,11 Magnesium, % 0,05 Sodium, % 0,04 Phosphorus, % 0,05 Iron, mg/kg 41 Copper, mg/kg 12 Zinc, mg/kg 4,3

5.0% OMW Number of crops harvested 6 5 4 3 2 1 0 Z0AP0 Z0AP1 Z0AP2 Z1AP0 Z1AP1 Z1AP2 Z2AP0 Z2AP1 Z2AP2 By increasing waste dose, the number of crops were increased. The highest waste dose resulted in the highest number of crops Zeolite seems to affect the number of crops For no waste case, increased zeolite percentage seems to increase crops number, but.. Zeolite and waste effect : Delays in crops growth The first crops were harvested with two weeks delay

Επίδραση των μεταχειρίσεων στο νωπό (FWT) και Crops Fresh (FWT) and Dry Weight (DWT) ξηρό βάρος (DWT) των καρπών 100 80 60 40 20 0 Z0AP0 Z0AP1 Z0AP2 Z1AP0 Z1AP1 Z1AP2 Z2AP0 Z2AP1 Z2AP2 FWT DWT

Substrate Organic Matter OM, % Treatments But if only the increased waste amount increases yield, is zeolite necessary?

Potassium in leachates WEEKS WEEKS With 0% zeolite: increased leaching as waste dose increases As zeolite dose is increasing, leaching is inhibited, therefore 1. Prevent leaching of nutrients 2. Keep nutrients in soil

Exchangeable Potassium in the substrate K, cmol/kg K, cmol/kg Treatments Treatments

Substrate Electrical Conductivity but this results to higher substrate EC EC, ms/cm Treatments

Electrical Conductivity, ms/cm WEEKS Although effects of waste and zeolite doses can be seen, in general the EC of the leachates are considered low...and also in comparison with what is happening when fertilizers are added on soils!!! WEEKS

Boron in Substrates B, mg/kg Treatments

Avalable Fe in Substrates Fe, mg/kg Treatments

WEEKS High Na concentrations released mainly during the first 3 weeks, which then reduced. It is due to zeolite, and increases as zeolite dose Increases. However, ESP remains < 6 (no salinity risk) but it affects production (e.g. Delays, WEEKS less crops) (Na 0.2 K 0.6 Mg 0.7 Ca 2.0 Al 6.2 Si2 9.8 O 72.19.6H 2 O

Sodium in Substrates Na, cmol/kg Treatments Organic matter of OMW increases the number of crops harvested and also the fresh and dry weight of the crops Zeolite prevent leaching of potassium and keep K in the substrate The EC of the substrate increases but t s not considered riksy for the 2.5% of zeolite and waste It is necessary to foresee a period of 2 3 weeks to wash Na from the substrate Compared to fertilization, the substrate used produced leachates with significant lower Electrical conductivity Because of the high Na content, the increased EC of soil and substrate, the delays in crop growth, the case of Z1AP1 or Z1AP2 (?) seem to be more appropriate, but measurements in crops are still in progress

Thank you for your attention Dr. Maria K. Doula Benaki Phytopathological Institute Greece