Exploring Creation. Sample. with. Biology. 2 nd Edition. by Dr. Jay L. Wile and Marilyn F. Durnell. Used by Permission

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Exploring Creation with Biology 2 nd Edition by Dr. Jay L. Wile and Marilyn F. Durnell

Exploring Creation With Biology, 2 nd Edition Published by Apologia Educational Ministries, Inc. 1106 Meridian Plaza, Suite 220 Anderson, IN 46016 www.apologia.com Copyright 2005 Apologia Educational Ministries, Inc. All rights reserved. Manufactured in the United States of America Seventh Printing, March 2011 ISBN: 978-1-932012-54-5 Printed by Courier, Inc, Kendallville, IN Cover photos: zebra (Dawn Strunc), cell Illustration( Imagineering I Custom Medical Stock Photo), leaf in background( Creatas, Inc.), cells in background( Brand X Pictures) Cover design by Kim Williams 11

Exploring Creation With Biology Table of Contents MODULE #1: Biology: The Study of Life... I Introduction........................ 1 What Is Life?........................................ 1 DNA and Life.................................................... 1 Energy Conversion and Life.................. 2 Sensing and Responding to Change........... 6 All Life Forms Reproduce............................. 7 Life's Secret Ingredient......................... 8 The Scientific Method............... 9 Limitations of the Scientific Method................. 12 Spontaneous Generation: The Faithful Still Cling to It!........... 15 Biological Classification....................... 16 Characteristics Used to Separate Organisms into Kingdoms........................ 18 The Definition of Species............ 20 Biological Keys..................... 21 Experiment 1.1: Using a Biological Key............................... 24 Naming Organisms Based on Classification.................................. 27 Alternate Forms of Taxonomy.............. 27 The Microscope........................ 30 Experiment 1.2: Introduction to the Microscope.................... 30 MODULE #2: Kingdom Monera... 37 Introduction................................ 37 Bacteria............................ 37 The Eating Habits of Bacteria..................... 41 Asexual Reproduction in Bacteria..................... 44 Genetic Recombination in Bacteria................. 47 Transformation and Transduction.................. 49 Endospore Formation....................... 50 Bacterial Colonies.................................... 50 Experiment 2.1: Pond Life, Part A.............. 52 Classification in Kingdom Monera..................................... 53 Classes in Kingdom Monera................ 54 A Few Words on Other Classification Systems.............. 56 Specific Bacteria............ 56 Conditions for Bacterial Growth......... 58 Preventing Bacterial Infections................................... 59 Take a Look at the Microscopic World................ 60 Experiment 2.2: Pond Life, Part B.................... 60 vm

MODULE #3: Kingdom Protista... 67 Introduction..................... 67 Experiment 3.1 : Pond Life, Part C.................. ~7 Classification in Kingdom Protista........................ 68 Sub kingdom Protozoa............................. 71 Phylum Sarcodina............... 71 Other Sarcodines....................... 73 Phylum Mastigophora................. 74 Other Mastigophorites.............. 75 Phylum Ciliophora....................... 78 Other Members of Phylum Ciliophora.................... 79 Phylum Sporozoa.................................. 80 Experiment 3.2: Subkingdom Protozoa........................ 82 Subkingdom Algae............................. 84 Phylum Chlorophyta.............................. 85 Phylum Chrysophyta........................ 87 Phylum Pyrrophyta......... 88 Phylum Phaeophyta................. 89 Phylum Rhodophyta.................... 91 Experiment 3.3: Subkingdom Algae................. 91 Summing Up Kingdom Protista............ 92 MODULE #4: Kingdom Fungi... 97 Introduction..................... 97 General Characteristics of Fungi............ 97 Reproduction in Kingdom Fungi............... 101 Classification in Kingdom Fungi............ 102 Phylum Basidiomycota......... 103 Other Members of Phylum Basidiomycota........................ 106 Experiment 4.1: Phylum Basidiomycota...... 107 Phylum Ascomycota.................... 109 Yeasts............... 109 Experiment 4.2: Yeast and the Fermentation Process................ 110 Other Members of Phylum Ascomycota.................. 111 Phylum Zygomycota..................... 112 Experiment 4.3: Molds................. 114 Phylum Chytridiomycota......... 115 Phylum Deuteromycota: The Imperfect Fungi............ 115 Optional Experiment 4.4: Imperfect Fungi...... 116 Phylum Myxomycota........... 117 Symbiosis in Kingdom Fungi................. 119 Summing Up Kingdom Fungi............... 120 Used by IX Permission

MODULE #5: The Chemistry of Life... 125 Introduction...................... 125 Atoms: The Basic Building Blocks of Matter..................... 125 Elements...... 128 Molecules..................... 130 Changes in Matter......................... 132 Physical Change.............................. 133 Experiment 5.1: Diffusion........... 134 Experiment 5.2: Osmosis................. 135 Chemical Change........................ 13 9 Photosynthesis......... 140 Organic Chemistry........................ 142 Carbohydrates........................ 142 Organic Acids and Bases......... 146 Lipids.............. 148 Proteins and Enzymes..................... 149 Experiment 5.3: The Fragility of an Enzyme... 152 DNA................................................. 154 MODULE #6: The Cell... 161 Introduction............. 161 Cellular Functions........................... 161 Cell Structure............ 164 The Cell Wall..................... 165 The Plasma Membrane.......................... 165 The Cytoplasm............... 166 The Mitochondrion............... 167 The Lysosome........................... 167 Ribosomes.................. 168 The Endoplasmic Reticulum........... 168 The Plastids............... 168 Vacuoles and Vesicles............... 169 Golgi Bodies............ 171 Centrioles.......................... 172 The Nucleus.......................... 172 The Cytoskeleton.................... 173 As If This Isn't Already Complicated Enough!..................... 174 Experiment 6.1: Cell Structure I............ 175 How Substances Travel In and Out of Cells............ 176 Experiment 6.2: Cell Structure II........... 181 How Cells Get Their Energy....................... 182 ATP and ADP............ 186 X

MODULE #7: Cellular Reproduction and DNA... 195 Introduction....................... 195 Genes, Chromosomes, and DNA...................... 1 ~5 Experiment 7.1: DNA Extraction.................. 197 Protein Synthesis - Part 1: Transcription................... 198 Protein Synthesis - Part 2: Translation... 201 Mitosis: Eukaryotic Asexual Reproduction........ 205 Experiment 7.2: Mitosis.............................. 210 Diploid and Haploid Cells.......................... 21 1 Meiosis: The Cellular Basis of Sexual Reproduction.............. 213 Viruses....................... 218 MODULE #8: Mendelian Genetics... 227 Introduction....................... 227 Gregor Mendel......... 227 Mendel's Experiments................................................ 228 Updating the Terminology...................... 233 Punnett Squares............................ 236 Pedigrees..................... 23 8 Experiment 8.1: Making Your Own Earlobe Pedigree........................ 241 More Complex Crosses........................ 242 "Experiment" 8.2: A Dihybrid Cross......................... 246 Sex and Sex-Linked Genetic Traits................. 247 "Experiment" 8.3: Sex-Linked Genetic Traits..................... 249 A More Complete Understanding of Genetics......................... 250 Genetic Disorders and Diseases...................... 252 Summing Up.......................... 255 Experiment 8.4: Environmental Factors and Their Effect on Radish Leaf Color..... 255 MODULE #9: Evolution: Part Scientific Theory, Part Unconfirmed Hypothesis... 261 Introduction................................. 261 Charles Darwin............................... 262 Darwin's Theory.............................. 264 Microevolution and Macroevolution......................................... 267 Inconclusive Evidence: The Geological Column............... 270 The Details of the Fossil Record: Evidence Against Macroevolution...... 273 The Cambrian Explosion............... 280 Structural Homology: Formerly Evidence for Macroevolution, Now Evidence against It..... 282 Molecular Biology: The Nail in Macroevolution's Coffin................... 285 Macroevolution Today............ :........... 289 Why Do So Many Scientists Believe in Macroevolution?......... 293 Used by Xl Permission

MODULE #10: Ecology... 299 Introduction....................................... 299 Energy and Ecosystems.................. 301 Mutualism.................................... 305 The Physical Environment................................ 309 The Water Cycle............................... 311 The Oxygen Cycle................................... 314 The Carbon Cycle..................... 316 Experiment 10.1: Carbon Dioxide and the Greenhouse Effect........ 317 The Nitrogen Cycle.......................... 322 Summing Up........................ 324 MODULE #11: The Invertebrates of Kingdom Animalia... 329 Introduction............... 329 Symmetry....................................... 329 Phylum Porifera: The Sponges.................. 332 Experiment 11.1: Observation of the Spicules of a Sponge.............. 334 Phylum Cnidaria..................... 335 Specific Members of Phylum Criidaria................. 337 Experiment 11.2: Observation of a Hydra...... 339 Phylum Annelida....................... 342 Feeding Habits of the Earthworm........................ 343 The Respiratory and Circulatory Systems in an Earthworm............ 344 The Earthworm's Reproductive System......... 345 Other Segmented Worms........................ 346 Experiment 11.3: Earthworm Dissection.............. 347 Phylum Platyhelminthes: The Planarian.................... 350 Experiment 11.4: Observation of a Planarian..................... 351 Other Members of Phylum Platyhelminthes............ 352 Phylum Nematoda............ 352 Phylum Mollusca........................... 354 Summing Up the Invertebrates................... 356 MODULE #12: Phylum Arthropoda... 361 Introduction........................................ 361 General Characteristics of Arthropods................ 361 Class Crustacea: The Crayfish.............. 365 The Crayfish's Respiratory System.................................... 366 The Crayfish's Circulatory System................. 368 The Crayfish's Digestive System................. 370 The Crayfish's Nervous System.............................. 370 The Crayfish's Reproductive System.................. 371 Other Crustaceans....................... 371 Experiment 12.1: Crayfish Dissection............................. 373 Class Arachnida.................... 376 Xll

The Spider............ 377 The Major Points oflnterest in Spider Anatomy...... 378 Classes Chilopoda and Diplopoda... 380 Class Insecta.................. 3 81 The Basic Anatomy of an Insect............... 3.82 Respiration and Circulation in Insects...... 382 The Feeding Habits of Insects...... 3 83 Reproduction and Development in Insects............... 383 A Few Orders in Class Insecta........... 385 Experiment 12.2: Insect Classification...... 389 MODULE #13: Phylum Chordata... 393 Introduction.................. 393 Subphylum Urochordata................ 394 Subphylum Cephalochordata....................... 395 Subphylum Vertebrata.............. 396 The Endoskeleton......... 396 The Circulatory System............. 399 The Nervous System............... 399 Reproduction......................... 401 Class Agnatha......... 403 Class Chondrichthyes............ 404 Class Osteichthyes............ 409 The Diversity of Class Osteichthyes.................. 414 Experiment 13.1: Perch Dissection............ 416 Class Amphibia............ 419 Specific Creatures in Class Amphibia............... 421 Experiment 13.2: Frog Dissection... 422 Alternate Experiment For Module #13 : Field Study II......... 422 Summing Up........ 423 MODULE #14: Kingdom Plantae: Anatomy and Classification... 429 Introduction... 429 Basic Plant Anatomy............ 429 The Macroscopic Structure of a Leaf............ 431 Experiment 14.1: Leaf Collection and Identification...... 435 The Microscopic Structure of a Leaf........................ 436 Leaf Color....:...... 438 Experiment 14.2: How Anthocyanins and ph Help Determine Leaf Color............. 439 Roots........................... 442 Stems.................... 446 Experiment 14.3: Cross Sections of Roots, Stems, and a Leaf........... 449 Classification of Plants... 452 The Bryophytes........ 452 Seedless Vascular Plants... 455 Seed-Making Plants............... 457 Xlll

MODULE #15: Kingdom Plantae: Physiology and Reproduction... 463 Introduction........... 463 How a Plant Depends on Water...................... 463 Water Absorption in Plants......... 465 Water Transport in Plants.................. 466 Plant Growth.................................... 469 Insectivorous Plants.......................... 472 Reproduction in Plants..................................... 473 Vegetative Reproduction........... 4 73 Sexual Reproduction in Phylum Anthophyta.................... 475 Experiment 15.1 : Flower Anatomy.............................. 4 78 The Reproductive Process in Anthophytes, Part 1: Forming Pollen and Embryo Sacs......... 480 The Reproductive Process in Anthophytes, Part 2: Pollination......... 482 The Reproductive Process in Anthophytes, Part 3: Fertilization..... 484 Seeds and Fruits.................. 485 Experiment 15.2: Fruit Classification........ 487 Germination and Early Growth......... 489 MODULE #16: Reptiles, Birds, and Mammals... 495 Introduction....................... 495 Class Reptilia..................... 495 Classification of Reptiles.................... 498 Order Rhynchocephalia......................... 499 Order Squamata.................. 499 Lizards............................. 500 Snakes...................... 501 Order Testudines.................... 503 Order Crocodilia.......................... 504 Dinosaurs............... 505 Class Aves............................. 507 Experiment 16.1: Bird Embryology.......................... 508 A Bird's Ability to Fly................... 509 Classification in Class Aves........................ 514 Experiment 16.2: Bird Identification...... 517 Class Mammalia......................... 518 Classification in Class Mammalia... 520 Summing It All Up............................... 526 Glossary... 531 Appendix A... 543 Appendix B... 545 Appendix C... 577 Index... 583 XIV

MODULE #1: Biology: The Study of Life Introduction In this course, you're going to take your fust detailed look at the science of biology. Biology, the study of life itself, is a vast subject, with many subdisciplines that concentrate on specific aspects of biology. Microbiology, for example, concentrates on those biological processes and structures that are too small for us to see with our eyes. Biochemistry studies the chemical processes that make life possible, and population biology deals with the dynamics of many life forms interacting in a community. Since biology is such a vast field of inquiry, most biologists end up specializing in one of these subdisciplines. Nevertheless, before you can begin to specialize, you need a broad overview of the science itself. That's what this course is designed to give you. What Is Life? If biology is the study of life, we need to determine what life is. Now to some extent, we all have an idea of what life is. If we were to ask you whether or not a rock is alive, you would easily answer "No!" On the other hand, if we were to ask you whether or not a blade of grass is alive, you would quickly answer "Yes! " Most likely, you can intuitively distinguish between living things and nonliving things. Even though this is the case, scientists must be a little more deliberate in determining what it means to be alive. Thus, scientists have developed several criteria for life. Not all scientists agree on all of these criteria, but in general, most biology courses will list at least some of the following criteria for life: 1. All life forms contain deoxyribonucleic ( dee ahk' see rye boh noo klay' ik) acid, which is called DNA. 2. All life forms have a method by which they extract energy from the surroundings and convert it into energy that sustains them. 3. All life forms can sense changes in their surroundings and respond to those changes. 4. All life forms reproduce. If something meets all of these criteria, we can scientifically say that it is alive. If it fails to meet even one of the criteria, we say that it is not alive. Now if you're not sure exactly what each of these criteria means, don't worry. We will discuss each of them in the next few sections of this module. DNA and Life Our first criterion states that all life contains DNA. Now we 're sure you've at least heard about DNA. It is probably, however, still a big mystery to you at this point. Why is DNA so special when it comes to life? Basically, DNA provides the information necessary to take a bunch of lifeless chemicals and turn them into an ordered, living system. Suppose, for example, we were to analyze an organism and determine every chemical that made up the organism. Suppose further that we went into a laboratory and made all of those chemicals and threw them into a big pot. Would we have made

2 Exploring Creation With Biology something that is alive? Of course not. We would not even have made something that resembles the organism we studied. Why not? In order to make life, we must take the chemicals that make it up and organize them in a way that will promote the other life functions mentioned in our list of criteria for life. In other words, just the chemicals themselves cannot extract and convert energy ( criterion #2), sense and respond to changes (criterion #3), and reproduce (criterion #4). In order to perform those functions, the chemicals must be organized so that they work together in just the right way. Think about it this way: suppose you go to a store and buy a bicycle. The box says, "Some assembly required." When you get it home, you unpack the box and pile all of the parts on the floor. At that point, do you have a bicycle? Of course not. In order to make the bicycle, you have to assemble the pieces in just the right way, according to the instructions. When you get done with the assembly, all of the parts will be in just the right place, and they will work together with the other parts to make a functional bike. In the same way, DNA is the set of instructions that takes the chemicals which make up life and arranges them in just the right way so as to produce a living system. Without this instruction set, the chemicals that make up a life form would be nothing more than a pile of goo. However, directed by the information in DNA, these molecules can work together in just the right way to make a living organism. Now of course, the exact way in which DNA does this is a little complicated. Nevertheless, in an upcoming module, we will spend some time studying DNA and how it works in detail. Energy Conversion and Life In order to live, organisms need energy. This is why our second criterion states that all life forms must be able to absorb energy from the surroundings and convert it into a form of energy that will sustain their life functions. The production and use of this energy is called metabolism (muh tab' uh liz uhm). Metabolism - The sum total of all processes in an organism which convert energy and matter from outside sources and use that energy and matter to sustain the organism's life functions Metabolism can be split into two categories: anabolism (uh nab' uh lizm) and catabolism (kuh tab' uh lizm). Anabolism - The sum total of all processes in an organism which use energy and simple chemical building blocks to produce large chemicals and structures necessary for life Catabolism - The sum total of all processes in an organism which break down chemicals to produce energy and simple chemical building blocks Although these definitions might seem hard to understand, think about them this way: when you eat food, your body has to break it down into simple chemicals in order to use it. Once it is broken down, your body will either bum those simple chemicals to produce energy or use them to make larger chemicals. The entire process of breaking the chemicals down and then burning them to produce energy is part of your body's catabolism. Once your body has that energy, it will use some of it to take simple chemicals and build large, complex chemicals that are necessary for your body to work correctly. The process of making those complex chemicals from simple chemicals is part of your body's anabolism. As we progress throughout the course, we will discuss specific examples of

Module # 1: Biology: The Study of Life 3 anabolism and catabolism, and that will help you better understand the distinction between them. One way to remember these two definitions is to notice that "catabolism" has the same prefix as "catastrophe," so they both involve things being broken down. Obviously, then, the energy that an organism gets from its surroundings is important. Where does it come from? Ultimately, almost all of the energy on this planet comes from the sun, which bathes the earth with its light. When you take chemistry, you'll learn a lot more about light. For right now, however, all you need to know is that light is a form of energy and that it is the main energy source for all living organisms on our planet. Green plants ( and some other things you will learn about later) take this energy and, by a process called photosynthesis (foh' toh sin thuh' sis), convert that energy into food for themselves. Photosynthesis - The process by which green plants and some other organisms use the energy of sunlight and simple chemicals to produce their own food We'll be looking at photosynthesis in great detail in a later module. Thus, if the definition is a little confusing to you, don't worry about it. What you need to know at this point is that photosynthesis allows plants and certain other organisms to convert the energy of sunlight into food. Photosynthesis is a part of anabolism, because the organism takes simple chemicals and converts them into food, which is composed of larger chemicals. If plants and other photosynthetic organisms absorb their energy from the sun, where do other life forms get their energy? Well, that depends. Some organisms eat plants. By eating plants, these organisms take in the energy that plants have stored up in their food reserves. Thus, these organisms are indirectly absorbing energy from the sun. They are taking the energy from plants in the form of food, but that food ultimately came from sunlight. Organisms that eat only plants are called herbivores (ur' bih vorz). Herbivores - Organisms that eat only plants So you see that even though herbivores don't get their energy directly from sunlight, without sunlight there would be no plants, and therefore there would be no herbivores. If an organism does not eat plants, it eats organisms other than plants. These organisms are called carnivores (kar' nih vorz). Carnivores - Organisms that eat only organisms other than plants Even though carnivores eat other organisms, their energy ultimately comes from the sun. After all, the organisms that carnivores eat have either eaten plants or have eaten other organisms that have eaten plants. The plants, of course, get their energy from the sun. In the end, then, carnivores also indirectly get their energy from the sun. Finally, there are organisms that eat both plants and other organisms. We call these omnivores (ahm nih' vors). Omnivores - Organisms that eat both plants and other organisms Ultimately, of course, these organisms also get their energy from the sun.

4 Exploring Creation With Biology Think about what we just did in the past few paragraphs. We took a large number of the organisms that live on this earth and placed them into one of three groups: herbivores, carnivores, or omnivores. This kind of exercise is called classification. When we classify organisms, we are taking a great deal of data and trying to organize it into a fairly simple system. In other words, classification is a lot like filing papers. When you file papers, you place them in folders according to their similarities. In this case, we have taken many of the organisms on earth and put them into one of three folders based on what they eat. This is one of the most important contributions biologists have made in understanding God's creation. Biologists have taken an enormous amount of data and have arranged it into many different classification systems. These classification systems allow us to see the similarities and relationships that exist between organisms in creation. Figure 1.1 illustrates the classification system you have just learned. Giraffes eat only plants; they are herbivores. FIGURE 1.1 Herbivores, Carnivores, and Omnivores Tigers eat only meat. This makes them carnivores. Giraffe Photo by Dawn Strunc Tiger Photo Comstock, Inc. Woman eating Photo Tan Kian Khoon Humans eat both plants and meats; we are omnivores. In biology, there are hundreds and hundreds of different ways that we can classify organisms, depending on what kind of data we are trying to organize. For example, the classification system we just talked about groups organisms according to what they eat. Thus, organisms that eat similar things are grouped together. In this way, we learn something about how energy is distributed from the sun to all of the creatures on earth. This is not the only way we can classify organisms to learn how energy is distributed from the sun to all of the creatures on earth. We could, alternatively, classify organisms according to these groups: producers, consumers, and decomposers. Producers - Organisms that produce their own food Consumers - Organisms that eat living producers and/or other consumers for food

Module # 1: Biology: The Study of Life 5 Decomposers - Organisms that break down the dead remains of other organisms In this system, plants are producers because they make their own food from chemicals and the sun's light. Omnivores, herbivores, and carnivores are all consumers, because they eat producers and other consumers. Certain bacteria and fungi (the plural of "fungus"), organisms we'll learn about in detail later, take the remains of dead organisms and break them down into simple chemicals. Thus, these creatures are decomposers. Once the decomposers have done their job, the chemicals that remain are once again used by plants to start the process all over again. This classification scheme, illustrated in Figure 1.2, gives us a nice view of how energy comes to earth from the sun and is distributed to all creatures in God's creation. Energy goes from the producers to the consumers. A consumer like the giraffe eats the producers directly. FIGURE 1.2 Producers, Consumers, and Decomposers 0 Energy comes from the sun Producers use simple compounds and the sun's energy to make their own food. and goes to the producers. All illustrations except the sun by Megan Whitaker Sun illustration from www.clipart.com Producers can use the simple compounds produced by decay to help support their life functions. Decomposers like bacteria and fungi cause dead organisms to decay into simple compounds, which ferti lize the soil. This decay also adds certain gases to the air. Energy can be transferred from consumers like the giraffe to other consumers. A consumer like the lioness eats the organisms that ate the producers. When the consumers die, the energy goes to the decomposers.