Brachypodium distachyon,, a new model plant Richard Sibout
ALICE: Brachypodium seed stocks Leaf DNA M2 Phenotyping (visual and chemical) (Versailles) 6 seeds (plants) Mutagenesis M1 M2 seeds production Versailles Mutagenized seeds Leaf DNA M2 Tilling (URGV-Evry) 10 M2 seeds (plants) M3 seeds stocks (Versailles) M2 seeds stocks (Versailles) M2 Saccharification Leonardo Gomez (York-UK) M3 seeds stock Backup (York-UK)
Emergence stage Young plants not seedled Dead at early stage delay Devlopment strong delay Plantlet Visual phenotyping: From sawing to harvest albinos Vegetative stage Mature plants Whole Stem plant Leaf Shape Color Size Color Shape & arrangement Appearance Flowering stage Mature plants Spike culm Flowering time Color Organisation Height & appearance Architecture Tillering / Branching
Visual phenotyping: From sawing to harvest nb phenotypes no germination 234 dead dead dead at early stage 7 early devlopment 0 late devlopment 10 plant devlopment plant devlopment strong late devlopment 6 Erected plant 129 Fairly Erected 120 Poorly Erected 20 architecture compact 0 compact at the top 0 hyper compact 0 giant with long internodes 0 giant with normal internodes 0 long internodes 0 Small with less internodes 5 Plant architecture height and appearance small with Short internodes 16 miniature 13 dwarf(1/4-2/3 of N) 7 extrem dwarf 7 no tiller 1 tillers low tillers number 10 high tillers number 21 no branching 0 branching type low branching 0 strong branching 1 very narrow 8 narrow 23 size small 27 large 0 cholorotic 1 albinos 1 glossy 0 beige midrib 1 brown midrib 0 red midrib 0 beige margins 1 brown margins 0 red margins 0 color dark green 20 pale green 16 light green 25 light green stripes 1 vein/lam - veining 1 variegated 0 yellow 1 dégradé jaune à la base vert en haut de la 1 Virescent / Albescent 1 white upper leaf 8 leaf etiolated upper leaf 1 adherent 3 brittled 0 crinkled 9 drooped 0 mottled 9 necrosed 17 appearance spotted 0 stunted 0 tearing 0 thick 0 twisted 1 waxy 0 wilted 0 rhomboid-pointed 0 round 0 indented edge 1 distorted 0 shape and arrangements downcurling 0 margin raised 0 upcurling 1 coneshaped at leaf base 0 leaflets superimposed 0 thin 36 floppy 1 shape thick 1 stem fasciated 1 curved internode 10 color brown 2 Head Head Short internode between spikelets 2 early flowering 28 flowering time late flowering 4 Spikelet trapped in stem and upper leaf 1 no flower 0 low flower / spike 5 sterile flowers 4 flowers reproductive organs Abnnormal spike 20 white spike 20 partial white spike 21 cauliflower type inflorescence 0 abnormal all 0 beige 0 flower morphology pale 0 small 27 seed color large 0 crinkled 9 seed size crackled seed coat 0 wrinkled 0 shape extremely wrinkled 0 61 different phenotypes have been scored thin 36 floppy 1 stem shape thick 1 fasciated 1 curved internode 10 color brown 2 Head Head Short internode between spikelets 2 flowering time early flowering 28 late flowering 4 flowers reproductive organs flower morphology Spikelet trapped in stem 1 and upper leaf no flower 0 low flower / spike 5 sterile flowers 4 Abnnormal spike 20 white spike 20 partial white spike 21 cauliflower type inflorescence 0 abnormal all 0 beige 0 pale 0
Examples of mutants affected in leaf and stem development Erected Virescent Curved stems wt Leaf color Unbranched Dwarf
Examples of mutants affected in spike, leaf and stem formation Scorpion tail spikelets Short or long spikelets wt Aborted spikes Abnormal stems Brown-midrib leaf
Natural accessions are genetic resources for cell wall variability Bd21.3 Bd21 Bd3.1 Bd18