2009, 32 (2) : 10214 Journal of N anjing A gricultural U niversity http: / / nauxb1njau1edu1cn,,. b [ J ]., 2009, 32 ( 2) : 10214 b,, 3 (, 210095) : b ( 249) 5, ( P n ) Rubisco,, P n Rubisco b,, Rubisco, b 5 P n : b; ; ; ; Rubisco : Q945 : A : 100022030 (2009) 0220010205 Changes in photosyn thesis and chlorophyll fluorescence parameters during rice leaf senescence in low chlorophyll b mutant L I Rui, ZHOU W ei, LU W ei 3 (College of L ife Sciences, Nanjing Agricultural University, Nanjing 210095, China) Ab s tra c t: Low chlorophyll b rice mutant and wild type rice ( Zhenhui 249) were used to study the changes in net photosynthesis rate ( P n ), chlorophyll fluorescence parameters and relative content of Rubisco during leaf senescence. The results showed that af2 ter full expansion of leaves, P n, chlorophyll fluorescence parameters and relative content of Rubisco decreased significantly in both low chlorophyll b rice mutant and the wild type. P n in the mutant was higher than that of wild type. But there was no difference of chlorophyll fluorescence parameters between the mutant and wild type. Meanwhile, the decline of the relative Rubisco content was faster in the wild type than in the mutant, fested at the carbon assim ilation stage. so the effects of low chlorophyll b on senescence of the fifth leaf of rice was mainly mani2 Key wo rd s: low chlorophyll b; leaf senescence; net photosynthetic rate; chlorophyll fluorescence; relative content of Rubisco,,,,,, b, b, [ 1-4 ] b,,,, b,, [ 5-6 ] b,, b [ 7 ] Chen [ 8-9 ],, b Rubisco, b : 2007212229 :, :,,, E2mail: luw@njau1edu1cn, Tel: 025284395423
2, : b 11, 1 111 (O ryza sativa L1) 249 ( ) 249 b ( ) HgCl 2 15 m in,, 30 48 h, 24 h,,, 5 d (0 5 10 15 20 25 30 35 d) 5 1 112 (R SP ) [ 10 ],, 3 80% [ 11 ] 113 ( P n ) (APD ) L I2COR L I26400,, 8: 00 11 : 00, 25, 1 000 mol m - 2 s - 1 6, 3 50% [ 11 ] 114 PAM, 20 m in 0107 210 6 000 mol m - 2 s - 1 [ 12 ] : PS F v / F m = ( F m - F o ) / F m ; ( PS ) PS = ( F m - F s ) / F m ; qp = ( F m - F s ) / ( F m - F o ) ; qn = 1 - ( F m - F o ) / ( F m - F o ) ; ETR = PS ( Yield) ( PAR) AF 015 : AF (Ab2 sorbed PAR Fraction, AF) [ 13 ], 0186, 0156 3 115 R ub isco 015 g 5 3 ml 50 mmol L - 1 Tris2HCl ph 718 ( 10 mmol L - 1 MgCl 2, 10 mmol L - 1 DTT, 10 mmol L - 1 NaHCO 3, 1215% Glycerol), 10 000 g 10 m in 012 ml, ( 2% SDS, 10% Glycerol, 5% 2Mercap toetha2 nol, 1% B romophenol B lue), 3 m in, SDS2PAGE 4%, 12%, 160 V, 1 h M ini2p rotean B iorad CBB R2250, /, Rubisco Rubisco 116 Excel, O rigin715 2 211 a /b R SP P n APD 1,, a /b 35 d, 73189%, 52176%, 1715 d, 24 d, 5 5, 5 5 ; a /b, b,,, 2, 1,, 20 d, 35 d, 81149%, 76161%, 2715 d 2915 d,
12 32 1 ( a ) a /b ( b) F ig11 C hange s in to ta l ch lo rop hyll ( a ) a nd ch lo rop hyll a /b ( b) du ring na tu ra l se ne sce nce o f m u tan t a nd w ild typ e 2 d, SPSS, 212 32A, 0 25 d, F o,, F o PS, F o, PS F o, F o PS F v /F m, 2 P n, F v / F m F ig12 C hange in P n du ring na tu ra l se ne sce nce o f m u tan t ( 32B ),, a nd w ild typ e 35 817%, 811%, 3 F o F v /F m PS ETR qp qn F ig13 Change s in F o, F v /F m, PS, ETR, qp, qn du ring na tu ra l se ne sce nce o f m u tan t a nd w ild typ e rice le af
2, : b 13 32C, PS, 0 25 d, ( ETR) ( 32D), 0 25 d, PS ETR qp ( 32E), ( PQ ), qn PS,, 213 R ub isco qn ( 32E) qp qn, Calvin CO 2 [ 14 ], Rubisco [ 15 ] 4 Rubisco, Rubisco, Bandscan, 35 Rubisco 40%, 58%, Rubisco 3 4 5 R ub isco SD S 2PAGE F ig14 SD S 2PAGE e lec trop ho re sis ana lysis o f the fifth lea f o f m u ta n t and w ild typ e rice, b, PS,,, [ 4 ], b, F v / F m, F v / F m, PS b PS,, b,,, 5 P n, Rubisco, Rubisco [ 16-17 ] b,,,,,, Rubisco, ( ) [ 18 ] Rubisco Rubisco, [ 19 ] ;, Rubisco, : [ 1 ] Killough D T, HorlacherW R. The inheritance of virescent yellow and red p lant colors in cotton [ J ]. Genetics, 1933, 18: 3292333 [ 2 ] Falbel T G, Staehelin L A. Partial blocks in the early step s of the chlorophyll synthesis pathway: a common feature of chlorophyll b2deficient mutants [ J ]. Physiologia Plantarum, 1996, 97: 3112320
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