DART Presentation Brian Musselman BioCop & Confidence Workshop April 26-27 th ICT Prague
The Art of DART Everyone wants to knows what s is in their sample Most methods require significant sample prep to get answers DART simplifies the process by generating simple MS data.
The Beginning
Formulated Products Utilize Thermal Desorption Technique For small tablets you can subsequently load up to 10 tablets then analyze for content by DART-MS in under 2 minutes Vary the carrier gas temperature to thermally profile the product Small tablets are held it place by a spring loaded plate pushing the tablets against a template Large tablets can simply be placed in the tablet tray
DART-SVP with Angle Insert the Tablet Carrier onto the ET (Experiment Table) Vary the carrier gas temperature while scanning across the surface to thermally desorb layer after layer of product Operation at up to 45 degree angle for efficient desorption
Thermal Desorption Method Development Daytime Caplet 4 DART-ET Temperatures - Search for 3 Active Ingredients A 150 ºC A D 250 ºC A P D 350 ºC A P D 450 ºC
Night time Caplet DART-ET 350 ºC Dextromethorphan HBr 1 Chlorine Isotope Pattern Chlorpheniramine Maleate 0.3%
DART Analysis of Raw Milk C:\Xcalibur\...\stds_x2_350C_25k 9.11.2009 12:59:12 stds_x2_350c_25k #663 RT: 2,33 AV: 1 NL: 2,31E5 T: FTMS {0;0} + p NSI Full ms [55,00-400,00] 100 90 80 70 60 50 40 30 20 10 Dimethyl furfural Interference 127,0390 MEL [M+H] + - 0.085ppm 127,0727 127,1117 500ppb MELAMINE SPIKED LIQUID 0 126,90 126,95 127,00 127,05 127,10 127,15 127,20 127,25 C:\Xcalibur\...\stds_x2_350C_25k 9.11.2009 12:59:12 m/z stds_x2_350c_25k #931 RT: 3,23 AV: 1 NL: 1,86E5 T: FTMS {0;0} + p NSI Full ms [55,00-400,00] 100 90 80 70 Interference 127,0389 100ppb 60 50 40 30 20 MEL [M+H] + - 0.479ppm LOD ~ 70ppb 10 127,0726 127,1117 0 126,90 126,95 127,00 127,05 127,10 127,15 127,20 127,25 m/z
Matrix Effects Water vs Milk 2_5ppm_solvent_stdx3_matrix_stdx3_350... 9.11.2009 11:28:48 RT: 1,20-3,19 100 90 80 STANDARD SM: 7B IN WATER (melamine 2.5ppm) 1,73 2,00 NL: 1,18E7 m/z= 127,06-127,08 MS 2_5ppm_solvent _stdx3_matrix_st dx3_350c_25k 70 1,40 1,41 60 50 40 30 RAW MILK (melamine 2.5ppm) 20 10 2,36 2,64 2,66 2,67 2,96 0 1,2 1,4 1,6 1,8 2,0 2,2 2,4 2,6 2,8 3,0 Time (min)
Rapid Analysis of Liquids For liquids you can analyze 12 samples per minute at high speed for qualitative analysis Or slow the presentation speed to deliver more quantitative analysis Analysis of 64 samples of Virgin Olive Oil in 22 minutes looking for di- and triglyceride content
High Throughput with No Sample Prep 48 Samples in under 20 minutes
Oils Positive-Ion Spectral Extra Virgin Olive Oil Fingerprints 100% Pure Olive Oil Mild Olive Oil Pure Vegetable Oil Toasted Sesame Oil
Oils Negative-Ion Spectral Fingerprints Extra Virgin Olive Oil 100% Pure Olive Oil Pure Vegetable Oil Toasted Sesame Oil
Raw Materials The Tweezer Block permits analysis of irregular shaped objects Or determine the content of active ingredients in seconds
Simple Sample Preparation Mix Metal with Powder Position in Desorption Ionization Region
What does this look like? Magnetic Field to High
Acquiring the Sample? Magnetized nail insert into vial
Scanning Multiple Tips Array of Magnetized nails in 96 well format
Tylenol Cold Tablet Nighttime Caplet Acetaminophen 325 mg/caplet Phenylephrine HCl 5 mg/caplet Chlorpheniramine Maleate 2 mg/caplet
10% Tylenol Powder in Metal TIC irregular Mass chromatogram for each API s have consistent ratios Acetaminophen = A Phenylephrine = P Chlorpheniramine Maleate = C Relative abundance vary from sample to sample
Mustard Powder Mix 1% Mustard Powder with Magnetite Vortex 30 seconds Deposit 3mg aliquot on glass slide Analyze 450C 350C
Mustard Powder Replicat e analyses Intact triglycerid es at 450C 450C
Thyme Leaves Positive Ion MS 350 degree Centigrade Helium gas Negative Ion MS
Thyme Leaves MS and MS/MS
McCormick Curry Powder Positive Ion MS 350 degree Centigrade Helium gas Negative Ion MS
Expanding DART s Range Desorption is typically protonated molecule for positive ion and deprotonated for negative ion Thermally Stable Molecules Derivatized Molecules Making stable molecules in the DART experiment
Crushed TIC TAC Disaccharide?
Korean Ginseng from Capsule Mix metal w/powder - add TMAH
Ginsenioside Rb 1 THAH Derivative Sigma Standard 1 mg dispersed in metal 3 mg on slide Apply 5 ul 25% solution TMAH in methanol Analyze at 350C to complete insitu derivative
Ginsenioside Rb 1 MS/MS 1364
Utility of High Resolution for Very Rapid Analysis of Complex Mix Pesticide Mix 529
Automation of DART Analysis + + Need automated sampling mechanism Need efficient, less labor intensive data analysis
AUTOMATION HARDWARE DART ionization source positioned under the rail of a programmable CTC autosampler from LEAP Technologies DART sources is standard production Injector body is modified to accept a new mandrel capable of picking up pipettor tips Standard 96-well titer plates used to hold samples Custom tips developed to create more reliable sample positioning in the ionization zone Implementation of Thermo LC/MS permits integrates of sample information with Xcalibur software Customizable sampling routines can be completed
SYSTEM CONFIGURATION
TIP ACQUISITION
SAMPLING FROM PLATE
Applications of DART in Prep-HPLC R1 It was never separated on the prep-column Which fraction contains the bromo imp? Results Courtesy of W. Schafer, Merck, Ambient Pressure Desorption Ionization Mass Spectrometry In Support of Preclinical Pharmaceutical Development, submitted 10/2009
96 Well Plate Analysis CH 3 N N N H Exact Mass: 133.06 TIC oxidative enzymes SM (M+1=134) Prod (SM+16)
area DART Linearity on Agilent MSD 450000 400000 350000 300000 250000 200000 150000 100000 50000 0 M+1 extracted from scan SIM (M+1) = 221 150 0 0.1 0.2 0.3 0.4 0.5 0.6 mg/ml MW 220 Sensitive enough for to track product formation Semi-quantitative: allows chemist to quickly identify best hits on plate
DART-SVP 2-D Scanner Experimental Program and Results IonSense, Inc. December 4, 2009
2D Scanner for DART-SVP Domestic & Foreign Currencies
2D Scanning Method: Example Bank Note - Royal Bank of Penguins START END 10 scans total across the bank note with 1 cm spacing row to row Linear rail speed was set at 1 mm/s Could not scan the entire length of the bank notes with the current set-up www.schmi-dt.de/penguins/banknote.en.html
Well Circulated $1 Bill Bottom Right-hand Corner Cocaine easily detected off of the $1 bill at an intensity level of 7.7 E6.
$10 Used vs. New - Bottom Right-hand Corner USED $10 Bill NEW $10 Bill No presence of cocaine on the crisp $10 bill
Well Circulated $20 Bill Varying Cocaine Signal Top Right Center Bottom Right
Circulated 20 Note Fingerprint Ions Center Middle Right Bottom Right
Circulated 200 Czech Korunas Note Fingerprint Ions Dense Ink Area on Image of Jan Amos Komensky Bottom Right Corner
Conclusions IPOD Touch controlled Direct Analysis in Real Time provides a methods driven platform for solving problems