10.1071/FP11237_AC CSIRO 2012 Accessory Puliction: Functionl Plnt Biology 2012, 39(5), 379 393. Supplementry mteril Tle S1. Effect of wter regime nd genotype on different growth prmeters: spike dry mtter (Spike DM), culm dry mtter (Culm DM), lef dry mtter (Lef DM), root nitrogen content (Root N), flg lef nitrogen content (Flg lef N) nd spike nitrogen content (Spike N) For ech genotype nd tretment dt shown re the mens of the four replictions. Mens followed y different letters were significntly different (P < 0.05) y Tukey s test., well wtered plnts; wter stressed plnts; G: Genotype; T: Tretment; G T: genotype y tretment interction. The ssocited sum of squres type III nd proilities (ns, not significnt; * P < 0.05; ** P < 0.01; *** P < 0.001) re shown T G Spike Culm Lef Root Flg lef Spike DM DM DM N N N (g plnt 1 ) (g plnt 1 ) (g plnt 1 ) (%) (%) (%) KS194 0.78 2.20 0.61 1.48 3.58 3.13 KS230 1.07 2.10 0.72 1.22 3.28 3.55 c RIL2108 1.05 2.78 0.70 1.34 4.36 2.57 RIL2510 0.95 2.88 0.67 1.09 4.55 2.53 Men 0.96 2.49 0.67 1.28 3.94 2.95 KS194 0.30 1.27 0.23 0.81 3.17 c 2.58 KS230 0.39 1.62 0.40 c 0.80 2.44 3.15 c RIL2108 0.68 d 1.51 0.24 0.67 2.99 2.18 RIL2510 0.58 c 1.69 0.32 0.66 3.58 c 2.01 Men 0.49 1.52 0.30 0.73 3.05 2.48 ANOVA G 0.45*** 1.54*** 0.08** 0.28* 6.21*** 5.90*** T 1.81*** 7.44*** 1.14*** 2.40*** 6.44*** 1.74*** G x T 0.13 ns 0.75* 0.02 ns 0.12 ns 0.94* 0.04 ns
Tle S2. Effect of wter regime nd genotype on the root dry mtter weight (Root DM), nd the root length (Root L) in ech of the three different soil sections, s well s the totl vlues for oth trits through ll the soil sections For ech trit the numer following the cronym refers to the soil section where the trit ws estimted: 1 refers to soil upper section (0.00 0.26 m); 2 refers to soil middle section (0.26 0.52 m); nd 3 refers to soil ottom section (0.52 0.78 m), while T efore the cronym refers to the totl trit vlue through the three soil sections. Dt shown is the men of the four replictions of ech genotype in ech tretment. Mens followed y different letters were significntly different (P < 0.05) y the Tukey s test., well wtered plnts;, wter stressed plnts; G, genotype; T, tretment; G T, genotype y tretment interction. The ssocited sum of squres type III nd proilities ( * P < 0.05; ** P < 0.01; *** P < 0.001) re shown T G Root DM 1 Root DM 2 Root DM 3 Totl Root DM Root L 1 Root L 2 Root L 3 Totl Root L (g) (g) (g) (g) m m m m KS194 0.18 0.07 0.07 0.31 4.04 2.16 1.75 7.95 KS230 0.14 0.07 0.10 0.32 4.18 3.06 4.23 c 11.47 RIL2108 0.30 0.08 0.09 0.48 7.36 3.16 2.92 13.44 RIL2510 0.26 0.11 0.13 c 0.49 6.67 5.15 4.94 c 16.75 c Men 0.22 0.08 0.10 0.40 5.56 3.38 3.46 12.40 KS194 0.18 0.09 0.05 0.32 3.01 2.02 1.20 6.22 KS230 0.22 0.11 0.09 0.42 5.11 3.09 2.35 10.55 c RIL2108 0.25 0.10 0.09 0.44 4.28 2.22 1.86 8.36 RIL2510 0.32 0.13 c 0.10 0.55 6.57 c 3.25 2.54 12.36 c Men 0.24 0.11 0.08 0.43 4.74 2.65 1.99 9.38 ANOVA G 0.08*** 0.01*** 0.014*** 0.20*** 44.00*** 18.99*** 24.17*** 223.46*** T 0.00 ns 0.00*** 0.00** 0.01 ns 5.37* 4.31*** 17.39*** 73.33*** G T 0.020* 0.001 ns 0.001 ns 0.02 ns 17.44** 4.64*** 4.07* 24.33*
RWD (g m -3 ) 450 400 350 300 250 200 150 () 100 50 RLD (m m -3 10-4 ) 0 1.0 0.9 0.8 0.7 0.6 0.5 0.4 0.3 () c 0.2 0.1 0.0 45 (c) SRL (m g -1 ) 40 35 30 25 20 15 10 5 0 KS194 KS230 RIL2108 RIL2510 Genotypes Fig. S1. Averged vlues through ll the soil sections for () root weight density (RWD), () root length density (RLD) nd (c) specific root length (SRL) for genotypes KS194, KS230, RIL2108 nd RIL2510. Dt include oth well-wtered (, white rs) nd wterstressed (, lck rs) plnts. Errors r represent the stndrd error of the men (SEM). Mens followed y different letters were significntly different (P < 0.05) y Tukey s test. Genotype, tretment, nd genotype y tretment interction were significnt for ll trits except for RWD the tretment ws not significnt.
-26.4-27.2 (), r 2 =0.86**** Overll, r 2 =0.57**** δ 13 C ( ) -28.0-28.8-29.6-30.4 32.5 32.0 31.5 (), r 2 =0.45**** Overll, r 2 =0.42**** δ 18 O ( ) 31.0 30.5 30.0 29.5 29.0 1.5 2.0 2.5 3.0 3.5 4.0 WUE Aeril DM Fig. S2. Reltionships etween plnt time-integrted wter use efficiency (WUE Aeril DM ) versus () δ 13 C of the spike nd () δ 18 O of the spike. Dt include oth well-wtered (, open circles) nd wter-stressed (, filled circles) plnts. The fitting line is only included for the significnt reltionships. r 2 nd proility is shown: **** P < 0.0001.
-30.0-30.5-31.0 δ 13 C ( ) -31.5-32.0-32.5-33.0-33.5 27 28 29 30 31 32 33 δ 18 O ( ), r 2 =0.41**, r 2 =0.63*** Overll, r 2 =0.73**** Fig. S3. Simple liner regression etween flg lef δ 18 O nd δ 13 C. Correltion dt include oth well-wtered (, open circles) nd wter-stressed (, filled circles) plnts. The fitting line is only included in the significnt reltionships. r 2 nd proilities re shown : ** P < 0.01; *** P < 0.001; nd **** P < 0.0001).
6 () () (c) Aeril DM (g plnt -1 ) Aeril DM (g plnt -1 ) 5 4 3 2 1 100 200 300 400 500 600 6 5 4 3 2 RWD 1 (g m -3 ) RLD 1 (m m -3 10-4 ), r 2 =64***, r 2 =0.54**** 60 100 140 180 220 RWD 2 (g m -3 ), r 2 =0.39** 20 60 100 140 180 220 RWD 3 (g m -3 ), r 2 =0.54***, r 2 =0.32* 1 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 6 (d) (e) (f) RLD 2 (m m -3 10-4 ), r 2 =0.25* (g) (h) (i) RLD 3 (m m -3 10-4 ), r 2 =0.52*** Aeril DM (g plnt -1 ) 5 4 3 2 Overll, r 2 =0.35*** Overll, r 2 =0.58**** 1 5 15 25 35 15 25 35 45 55 SRL 1 (g m -1 ) SRL 2 (g m -1 ) 15 25 35 45 55 SRL3 (g m -1 ) Overll, r 2 =0.40**** Supplementry Figure
Fig. S4. Reltionships etween: root weight density (RWD, upper: c), root length density (RLD, middle: d f) nd specific root length (SRL, lower: g i) versus eril dry mtter (Aeril DM). The numer ehind the trit refers to the soil section where the trit ws estimted: 1, upper section (0.00 0.26 m); 2, middle section (0.26 0.52 m); nd 3, soil ottom section (0.52 0.78 m). Dt include oth well-wtered (, open circles) nd wter-stressed (, filled circles) plnts. The fitting line is only included in the significnt reltionships. r 2 nd proilities re shown: * P < 0.05; ** P <0.01; *** P < 0.001; nd **** P < 0.0001.
-25.5-26.5 (), r 2 =0.57*** Overll, r 2 =0.54**** δ 13 C ( ) -27.5-28.5-29.5-30.5 34 (), r 2 =0.31*, r 2 =0.26* 33 Overll, r 2 =0.36*** δ 18 O ( ) 32 31 30 29 0 1 2 3 4 5 6 7 T cum / TRoot DM Fig. S5. Reltionship etween the plnt cumultive trnspirtion per unit of Root DM (T cum /TRoot DM) versus () δ 13 C nd () δ 18 O of the spike. Dt include oth well-wtered (, open circles) nd wter-stressed (, filled circles) plnts. The fitting line is only included in the significnt reltionships. r 2 nd proilities re shown: * P < 0.05; *** P < 0.001; nd **** P < 0.0001.