Agilent InfinityLab Solutions EXTEND YOUR APPLICATION REACH WITH MAXIMUM EFFICIENCY
INTRODUCTION EXTEND YOUR APPLICATION REACH WITH MAXIMUM EFFICIENCY Agilent s new supercritical CO 2 -based technologies deliver robust, reliable, and reproducible separation results for diverse sample types with the widest selectivity from polar to nonpolar. What s more, Agilent InfinityLab Solutions maximize efficiency, use less solvents, and lower costs while being fully compatible with the full range of Agilent mass spectrometers. 2 15 1 5.5 1. 1.5 2. UHPLC 1 8 6 4 2 HPLC ANALYTICAL EFFICIENCY Unleash the power of. Separate at high flow rates up to 6 bar. Achieve analysis speeds up to 1-times faster than HPLC. INSTRUMENT EFFICIENCY Exploit the full orthogonality of supercritical fluid chromatography. Compare and UHPLC results on one system. LABORATORY EFFICIENCY Reduce costs of solvent purchase and waste disposal by a factor of 5. Eliminate toxic solvents. Make your lab leaner and greener. 2
COMPLETE SOLUTIONS Agilent InfinityLab Solutions are truly complete end-to-end solutions, combining superior instruments and columns, smart supplies, intuitive software, and dedicated services. InfinityLab Instruments, Columns and Supplies Designed to work together in perfect harmony, Agilent InfinityLab instrumentation and single quadrupole mass selective detectors combine with Agilent columns and InfinityLab supplies to maximize the efficiency of your workflows. Services and Support Learn how to gain the maximum benefit from your InfinityLab Solutions through our comprehensive suite of services and learning solutions. Software and Informatics Stay in full control of all your applications through OpenLAB CDS software, and optimize development of your methods with Method Scouting Wizard. 3
TECHNOLOGY EXPERIENCE ROBUST PERFORMANCE FOR HIGHEST CONFIDENCE IN YOUR DAILY RESULTS InfinityLab Solutions are fully-fledged members of the InfinityLab family. While some modules have been reengineered to cater for the demands of liquefied CO 2 as mobile phase, many modules remain unchanged and are common to both and UHPLC systems. EFFICIENT COLUMN HANDLING Hands-free selection of up to four columns. Independent heating zones for optimum separation and postcolumn conditioning. Agilent 126 Infinity II /UHPLC Hybrid System Switch between and UHPLC with a simple mouse click! With the easy-to-install addition of a 2-position/1-port valve and a 126 Infinity II LC Pump, your system becomes a fully operational hybrid /UHPLC solution. The option of running both and UHPLC applications on the same system allows you to maximize the utilization of your analytical equipment. The core of the hybrid solution is the 126 Infinity II Multisampler. With feed injection for sampling and classic flow-through injection for UHPLC sampling, the Multisampler is flushed automatically when switching between techniques. UHPLC HYBRID, MULTIPURPOSE SAMPLING New, unique feed injection for sampling. Classic flowthrough injection for UHPLC sampling. Flexible choice of sample containers such as 2 or 6 ml vials, or microtiter plates. Highest capacity up to 432 samples in 2 ml vials. feed injection Extended injection volume range from.1 to 9 µl Full choice of sample transfer solvent and injection speed No delay volume Reduced sample solvent effects SUPERIOR SOLVENT DELIVERY Highest flow accuracy and precision up to 5 ml/min at 6 bar. Improved usability and extended lifetime through integrated degasser, solvent selection, valve and active seal wash. 4
PERFECTLY MATCHED SUPPLIES Smaller footprint, easy-to-grip solvent bottles with Stay Safe caps make solvent handling more convenient just two of the many InfinityLab LC supplies that help deliver reliable, robust performance. EXTENDED APPLICATION REACH Check out our broad range of detection options, see page 1. CONVENIENT CO 2 HANDLING Programmable backpressure gradients for shorter analysis times. Full-flow transfer to MS or ELSD with new lowdispersion nozzle. Increased robustness and extended lifetime through major overhaul of booster pump. 5
TECHNOLOGY DEVELOP YOUR METHODS WITH MAXIMUM EFFICIENCY InfinityLab Method Development Solutions comprise instruments, columns, software, and services that work together in perfect harmony, helping you to develop your chromatographic methods with maximum efficiency. Dedicated hardware for the highest degree of automation The flexibility of the InfinityLab solutions helps you to solve your toughest development challenges. Simply install an external solvent-selection valve and gain immediate, automated access to 15 different solvents. Cluster two, three, or even four 129 Infinity II Multicolumn Thermostats for automated screening of up to 32 columns. 15 32 Easy, automated access to columns and solvents gives you ultimate flexibility in method development. Columns and supplies for simple and quick method development ZORBAX and InfinityLab Poroshell columns are manufactured end-to-end by Agilent and monitored with multiple QC tests, so you can be assured of rugged, reproducible performance and long column lifetimes. From Quick Connect capillaries through Stay Safe caps, our wide range of parts, kits, and accessories are designed, manufactured, and tested under a quality system registered to ISO 91. Download our Quick Reference Guide for Supplies from www.agilent.com, search for 5991-8116EN. 6
Advanced tools for advanced applications When combining your solution with triple quadrupole or quadrupole time-of-flight mass spectrometers, Agilent MassHunter software is designed to make your MS analyses faster, easier, and more productive. MassHunter s modular architecture lets you integrate new features to match your workflow requirements. Dedicated software accelerates method development Agilent OpenLAB CDS ChemStation Edition provides the versatility you need to develop your methods. Agilent Method Scouting Wizard helps you to generate methods sequences for complex screening campaigns within minutes. Agilent MassHunter Walkup software allows users of different skill levels to perform their own and /MS analyses. Results show up in the submitter s in-box automatically. Method Scouting Wizard is an easy-to-use but highly effective tool to develop and UHPLC methods with less effort. Use this add-on for OpenLAB CDS ChemStation to reduce time spent on manual scanning of different parameters such as mobile phases and columns. The right tools for the job Whether you run a small lab with few instruments or a larger operation with a requirement for networked solutions, OpenLAB software products cover all demands. OpenLAB CDS 2 increases productivity in analytical QC labs and gives you peace of mind when regulatory agencies audit or inspect your operation. With support for and single quadrupole mass selective detection, OpenLAB CDS 2 helps you to: Deliver results quickly and securely by performing calculations and producing reports in the protected environment of the software Identify nonconforming samples quickly with Peak Explorer visualization tool and MatchCompare add-on OpenLAB CDS 2 One software for Agilent, LC, LC/MS, GC, GC/MS and other vendors instruments provides the same user experience and enables viewing data across techniques and instruments with the same user interface. 7
AT A GLANCE SWITCH TO AND PROTECT YOUR INVESTMENT AND THE ENVIRONMENT Switch to now and benefit from an immediate reduction in the costs of solvent purchase and waste disposal. What s more, the virtual elimination of hazardous solvents makes your lab a cleaner place to work as well as protecting the environment. : CO 2 and organic modifiers : CO 2, modifiers, additives, and water : pure CO 2 Normal-phase HPLC : CO 2, modifiers and additives HILIC Reversed-phase HPLC Ion chromatography Ion pairing INCREASING POLARITY Hydrocarbons Aldehydes and ketones Hydroxy acids and poly acids Peptides Ions Fatty acids and waxes Analine and benzoic acids Primary amines Proteins Ethers and esters Zwitter ions DNA/RNA Except for extremely polar separation techniques such as ion exchange chromatography, largely covers the same application areas as normal-phase LC, reversed-phase LC, and hydrophilic interaction chromatography (HILIC). The applicability of ranges from nonpolar hydrocarbons through high-polarity amines, hydroxy acids, and peptides. Leaner and greener but where can I use? As a fully orthogonal separation technology to liquid chromatography, has the potential to replace LC in a huge number of applications that span a wide range of analyte polarities. An impressive number of publications on completely diverse applications report as the superior choice to LC. The main area where delivers better results covers applications currently using normal-phase LC such as the analysis of chiral molecules. 8
A supercritical fluid is any substance at a temperature and pressure above its critical point, where distinct liquid and gas phases do not exist. Close to the critical point, small changes in pressure or temperature result in large changes in density. These characteristics make it possible to adjust many physical properties so that a supercritical fluid can be used as a substitute for organic solvents in industrial and laboratory processes such as chromatography. In supercritical fluid chromatography, carbon dioxide is the most commonly used solvent whereby cosolvents are added to modify the mobile phase polarity and thereby change the selectivity of the separation system. P [bar] 1, 1, 1 1 1 2 solid liquid triple point supercritical fluid critical point gas 25 3 35 4 T [K] Benefits at a Glance GHS classification Reduce hazardous solvents Replacing normal-phase LC by means a significant reduction in the need for hazardous solvents such as acetonitrile, dichloromethane, or heptane. Your lab becomes a greener place to work where your only concern is the GHS classification of typical modifiers such as methanol, ethanol, or isopropanol. Heptane NPLC Acetonitrile RPLC (+ modifier) Reduce solvent costs InfinityLab Solutions use food-grade carbon dioxide as the primary mobile phase. Food-grade CO 2 can be purchased at a fraction of the cost of the primary solvents used in other modes of liquid chromatography. Significant savings are also made in waste disposal with only small amounts of modifiers being used. Heptane NPLC Acetonitrile RPLC Cost per liter CO2 Reduce waste production The proportion of organic modifier used in gradients is generally significantly lower than in normal- or reversed-phase LC. As a result, generates far less waste. The major solvent, carbon dioxide, simply evaporates and diffuses safely into the atmosphere. 1 % NPLC 5-95 % RPLC Proportion of organic solvents 5-4 % 9
APPLICATIONS MATCH DETECTION TECHNIQUE TO YOUR APPLICATION NEEDS InfinityLab Solutions extend and broaden your application reach by providing an orthogonal separation mechanism that can be combined with a wide variety of different detection techniques. Choose a detector that matches your needs for selectivity and sensitivity, the number of analytes, and the complexity of your sample matrix. Flame ionization detection* 126 Infinity II Sensitive analysis of fuels by flame ionization, according to standard methods such as ASTM D5186. *SIM Scientific Instruments Manufacturer GmbH Evaporative light scattering detection 129 Infinity II ELSD Quantitative and qualitative analysis of nonvolatile analytes that lack UV-visible chromophores such as sugars. UV-visible detection 126 Infinity II DAD Determination of enantiomeric ratios or quantification of a small number of known analytes. Full-flow transfer for highest sensitivity Full flow ELSD/MSD To achieve highest sensitivity, transfer the complete effluent containing your precious sample to the ELSD, MSD, or LC/MS. The new low-dispersion nozzle significantly reduces the adverse effects of passing through the backpressure regulator. 1
Number of analytes Mass selective detection InfinityLab MSD Simplified peak tracking in method development or quantification of a few analytes in simple matrixes. Triple quadrupole mass spectrometry 647 Triple Quadrupole LC/MS Highly sensitive forensic screening, confirmation, and quantification of drugs of abuse, pesticides, or vitamins in challenging matrixes. Quadrupole time-of-flight mass spectrometry 6545 Quadrupole Time-of-Flight LC/MS Full screening of suspect analytes or unknowns in complex matrixes using data-mining tools, and personal compound databases and libraries (PCDL). Flow splitting for highest resolution Split flow MS To achieve highest resolution, use a flow splitter in front of the backpressure regulator so that only a portion of the eluent is transferred to the detector. This setup keeps the dispersion volume to a minimum and reduces band broadening. 11
APPLICATIONS EXTEND YOUR APPLICATION REACH WITH STATE-OF-THE-ART InfinityLab Solutions allow you to separate and quantify complex mixtures faster and more efficiently than ever before. Deploying in your lab gives you a technique that is orthogonal to normal- or reversed-phase LC and enables you to reach beyond your current application spectrum. Use to separate your most challenging samples Vitamin E tocopherols and tocotrienols have different biological activities and chemical properties so it is important to identify and quantify each vitamer separately. enables complete resolution of all vitamers within a significantly shorter analysis time. Download Application Note from www.agilent.com, search for 5991-1546EN. mau 12 1 8 6 4 2 PG TBQH TROLOX BHA OG LG BHT δ-tt γ-tt α-tt δ-tp γ-tp α-tp UHPLC mau 8 7 6 5 4 3 2 1 BHT BHA α-tp α-tt β-tp γ-tp δ-tp γ-tt δ-tt TBQH TROLOX 2.5 5 7.5 1 12.5 15 17.5 2 22.5 min 2.5 5 7.5 1 12.5 15 min Separation and UV detection of 14 antioxidants found in vegetable oils by UHPLC (left) and by (right). The concentration of each antioxidant was 1 μg/ml. 12
Solve separation problems faster and more reliably with The E7 liquid crystal mixture can be separated and quantified within two minutes using with UV detection. This is up to 4-times faster than analysis based on normal-phase LC. Further, delivers improved peak shape for more reliable quantification. Download Application Note from www.agilent.com, search for 5991-6436EN. mau 2 175 15 DAD1 A, Sig=28,4 Ref=36,1 5CB 1.385 7CB 1.468 8OCB 1.573 5CT 1.753 4-cyano-4'-n-pentyl-biphenly (5CB) CAS number: 4817-8-1 4-cyano-4'-n-heptyl-biphenyl (7CB) CAS number: 41122-71-8 H 3 C H 3 C C C N N 125 1 75 5 25 4-cyano-4'-n-oxyoctyl-biphenyl (8OCB) CAS number: 52364-73-5 4-cyano-4''-n-pentyl-terphenyl (5CT) CAS number: 54211-46- H 3 C H 3 C O C C N N.25.5.75 1. 1.25 1.5 1.75 2. 2.25 min Separation of a mixture of liquid crystal compounds 5CB, 7CB, 8OCB, and 5CT (5 μg/ml, each) within a short run time using a fast gradient. Benefit from unmatched injection precision with flexible injection volumes The new feed-injection principle achieves unmatched precision and linearity with negligible carryover. It provides full flexibility in the selection of injection volume and eliminates the disadvantages associated with the widely used fixed-loop injection technique. Download Technical Overview from www.agilent.com, search for 5991-7623EN. mau 25 2 15 1 5 8 µl, 7 µl, 6 µl 5 µl 4 µl 3 µl 2 µl 1 µl 3.2 3.4 3.6 3.8 4. 4.2 4.4 4.6 4.8 Feed injection delivers unprecedented area linearity over a wide volume range from.1 to 9 μl. min Peak area 3.2 3.4 3.6 3.8 4. 4.2 4.4 4.6 4.8 Linearity of peak area from large volume injection 1,6 1,4 y = 18.76x + 3.9655 R 2 =.9999 1,2 1, 8 6 4 2 1 2 3 4 5 6 7 8 Injection volume (µl) Peak area RSD (%) 13
APPLICATIONS THE PERFECT TOOL FOR METHOD AND COMPOUND SCREENING The analysis speed and the flexibility to handle large and small injection volumes make InfinityLab Solutions the perfect tool for method and compound screening. What s more, our systems combine seamlessly with high-end mass spectrometry. Develop and optimize new methods for your chiral separations The InfinityLab Method Development Solution with Agilent Method Scouting Wizard facilitates software-aided method scouting of four chiral columns to develop and optimize new methods. Download Application Note from www.agilent.com, search for 5991-7624EN. For Research Use Only. Not for use in diagnostic procedures. mau 25 2 15 1 4.495 Separation of propranolol enantiomers under isocratic conditions: 3 % MeOH 5.44 5.731 25 % MeOH 2 % MeOH Chiral column: IB 7.87 7.855 9.928 mau 3 25 2 15 1 3.85 3.561 5 5 2 4 6 8 1 min 2. 2.5 3. 3.5 4. min is a fast approach to identify speed-optimized methods for separation of racemates such as propranolol. The final optimized method is shown right. 14
Determine drugs of abuse at trace levels using with triple quadrupole MS is an ideal separation technique for fast, quantitative screening of different classes of drugs, ranging from simple amines to complex morphine-like structures such as those analyzed in forensic toxicology applications. Highest analytical sensitivity is achieved by combining with triple quadrupole mass spectrometry. Download Application Note from www.agilent.com, search for 5991-6747EN. For Forensic Use. 1 5 6. 2.957 5. Counts 4. 3. 2. 1..8 1. 1.2 1.4 1.6 1.8 2. 2.2 2.4 2.6 2.8 3. 3.2 3.4 3.6 3.8 4. 4.2 4.4 4.6 Acquisition time (min) separation and DMRM detection of 25 drugs within 5 minutes. The limit of detection (LOD) was 3 pg/ml. Identify and quantify more than 2 pesticides in a single /MS run The combined performance of and triple quadrupole MS makes it possible to identify and quantify over 2 pesticides in a single run. Using dynamic multiple reaction monitoring (DMRM) significantly improves the sensitivity of the analysis. Download Application Note from www.agilent.com, search for 5991-6151EN. 1 6 3. 2.5 Counts 2. 1.5 1..5 6.856 1 2 3 4 5 6 7 8 9 1 11 12 13 14 15 16 17 18 Acquisition time (min) Determination of 223 pesticides by DMRM. The first 195 compounds eluted within 1 minutes. 15
Learn more www.agilent.com/chem/sfc Find an Agilent customer center www.agilent.com/chem/contactus USA and Canada 1-8-227-977 agilent_inquiries@agilent.com Europe info_agilent@agilent.com Asia Pacific inquiry_lsca@agilent.com This information is subject to change without notice. Agilent Technologies, Inc. 217 Published in the USA, October 15, 217 5991-8326EN