29 2 Vol. 29, No. 2 2010 5 JOURNAL OF OCEANOGRAPHY IN TA IWAN STRA IT May, 2010 2001 2 6 1, 2, 2 (1., 510632; 2., 361005) : (RP2HPLC) 2001 2 6, CHEMTAX,. RP2HPLC, 1 /3,. RP2HPLC, 2001 2 6, ( fucoxanthin) 19 2 ( 19 2butanoyloxyfucoxanthin) 19 2 (19 2hexanoyloxyfucoxanthin) (peridinin), 267. 824 655. 076 20. 118 126. 764 65. 693 214. 115 26. 955 203. 927 g/m 3. CHEMTAX :,, 36. 14% 79. 27% 4. 50% 37. 40% 4. 83% 21. 37%. 2 6,.,. : ; ; ; DO I: 10. 3969 /J. ISSN. 100028160. 2010. 02. 012 : P 735 : A : 100028160 (2010) 0220228206,,,, [ 1 ].,,.,. (RP2HPLC),,,,., ( ) HPLC,. HPLC,,. CHEMTAX,, [ 2 ].,,,,.,, CHEMTAX HPLC., 2001 2 6 CHEMTAX,. : 2009206212 : ( 49806004 ) ; ( 51208049 ) ; (9451063201002243) : (1978 ),,, ; E2mail: hexuejia@hotmail. com : (1966 ),, ; E2mail: xypeng@ xmu. edu. cn
2 : 2001 2 6 229 1 1. 1, 7, 4 : (Bacillariophyceae) : ( Thalassiosira w eissfloyi) ; ( Prymnesiophycea) : ( Isochrysis ga lbana) ; (Chlorophycea) : (D una liella sp. ), (Ch lorella au totropica), ( P latym onas sp. ) ; (Cyanophyceae) : (Spirulina sp. ), (Synechococcus sp. ).. f/2, 20, 12 000 lx, 12 12; 1. 2,, ( P < 266hPa) (W hatman GF /C, = 47mm, Cat. No. 1825047). 1cm 3,,,. 1. 3 2001 2 6 ( 1) 3d. 5dm 3 1. 6dm 3, 130 m,. 1. 4, 5cm 3, 2Apo28 carotenol ( trans) ( Sigma2A ldrich, USA ), 5cm 3, 3 5m in, ( - 20 ) 24h. (W hatman GF /C, = 24mm) ( P < 266hPa), ( 2. 0 cm 3 ).,. 1. 5 HPLC HPLC Agilent HP1100 Series, Hewlett Packard, (DAD ) 440nm 300 750nm. : A: 0. 5mol/dm 3 = 65 20 15 (V /V, ph 7. 2) ; B: = 60 40 (V /V ). : 16m in, A B, 8m in,. 1. 6 DAD.. 1. 7 CHEMTAX (CHEMTAX 1. 95), ( D inophyceae ), ( Bacillariophyceae ), ( Prymnesiophycea ), (Chlorophyta) (Cyanophyceae). (1999) [ 3 ] Mackey(1996) [ 2 ].
230 29 2 2. 1 18 [ 2 2Apo28 carotenol ( trans) ] ( 1)., ( cis2fucoxanthin) a. a ( chlorophyllide a) b ( chlorophyllide b), ( Prasinophyceae) (Cryp tophyceae) (p rasinoxanthin) ( alloxanthin),,.,, ( TLC) (HPTLC), HPLC,,,,.,. HPLC (1999) [ 3 ] Nelson (1993) [ 4 ], A, 1 /3 ( 36m in 24m in), 18,,.,,,. 1 HPLC DAD Tab. 1 Pigments separated and identified using HPLC with characteristics measured using DAD /m in / nm 1 chlorophyll C1 + C 2 4. 40 C1 + C 2 445, 440, 469 CHL C1 + C 2 2 peridine 6. 30 445, 468 PER ID 3 19 2butanoyloxyfucoxanthin 6. 76 19 2 444, 467 BUT2FUCO 4 fucooxanthin 7. 28 452, 468 FUCO 5 neoxanthin 8. 00 417, 440, 469 NEO 6 violaxanthin 8. 25 418, 441, 470 V IOLA 7 19 2hexanoyloxyfucoxanthin 9. 15 19 446, 476 HEX2FUCO 8 cis2fucooxanthin 9. 50 cis2 446, 476 cis2fuco 9 diadinoxanthin 9. 94 447, 465 D IAD INO 10 alloxanthin 10. 21 420, 446, 473 ALLO 11 diatoxanthin 11. 00 420, 442, 470 D IATO 12 zeaxanthin 11. 20 452, 479 ZEA 13 lutein 11. 50 441, 469 LUT b2apo28 carotenol( trans) 13. 80 460 3-14 chlorophyll b 15. 72 b 446, 650 CHL b 15 chlorophyll a ( ep imer) 16. 75 a ( ) 432, 666 CHL a EP IM 16 chlorophyll a 17. 23 a 430, 666 CHL a 17 chlorophyll a ( allomer) 18. 18 a ( ) 430, 665 CHL a ALLOM 18 2carotene 19. 85 2 452, 476 2CAR : 3
2 : 2001 2 6 231 2. 2 2. 2. 1 HPLC, 18, ( zeanxanthin), 2., ( fucoxanthin) 19 2 ( 19 2butanoyloxyfucoxanthin) 19 2 ( 19 2 hexanoyloxyfucoxanthin) (peridinin), 267. 824 655. 076 20. 118 126. 764 5. 693 214. 115 26. 955 203. 927 g/m 3., 100 g/m 3. 2. 2. 2 CHEMTAX, a ( 3), ( 2). 2001 2 6 a 736. 685 1 570. 882 g/m 3, (2003) [ 5 ]. 2, 36. 14% 79. 27%,, 4. 50% 37. 40% 4. 83% 21. 37%, 6. 84% 14. 77%,. [ 5-6 ], 3 (53. 97% ) 1995 1996 : 55. 78% 59. 51% 47. 84% [ 6 ],,,. (2003) [ 5 ],,,.,, ( Prasinophyceae) (Cryp tophyceae),,., 6. 6. 2 5, 66. 79%, 6 79. 27%,, 6. a ( 2), 5,. 5 ( 291. 330 g/m 3 ). (1990) 6 [ 7 ],.,. 2 2001 2 6 Tab. 2 Concentrations of diagnostic carotenoids, chlorophyll and carotene in western Xiamen waters from February to June, 2001 / g m - 3 2 3 4 5 6 PER 26. 955 90. 073 48. 064 203. 927 89. 995 BUT2FUCO 20. 118 131. 310 105. 116 126. 764 0. 000 HEX 99. 967 103. 52 65. 693 214. 115 111. 110 FUCO 309. 288 493. 851 267. 824 363. 341 655. 076 NEO 30. 933 12. 261 10. 204 10. 727 14. 779 V IOLA 28. 062 20. 992 24. 897 37. 666 26. 598 D IAD INO 1. 295 1. 756 1. 610 1. 666 1. 605 D IATO 42. 226 25. 489 29. 817 60. 378 83. 584 ZEA 0. 000 0. 000 0. 000 0. 000 0. 000 LUT 7. 417 8. 8050 10. 465 22. 142 15. 398 CHL b 19. 399 19. 886 13. 511 43. 811 42. 015 2CAR 39. 813 35. 696 42. 657 130. 119 115. 012 CHL a 736. 685 1 186. 626 711. 899 1 363. 382 1 570. 882 : 2 9, 10
232 29 3 2001 2 6 a Tab. 3 B iomass ( chlorophyll a) of major phytop lankton group s of southern Xiamen waters from February to June, 2001 a / g m - 3 2 3 4 5 6 35. 577 123. 900 67. 203 291. 330 125. 854 492. 031 640. 476 288. 903 492. 695 1 245. 226 101. 883 341. 809 266. 256 416. 018 70. 723 108. 830 81. 137 91. 936 155. 345 127. 932 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 7. 993 1. 148 736. 685 1 186. 626 711. 899 1 363. 382 1 570. 882 2. 2. 3.,, 2 15. 4 5 25. 0 ( 3),,,.,,.,,,. 25 ( 3),,. 2 3 5 6 0. 69 0. 65 0. 50 0. 36 mg/dm 3, 0. 04 0. 03 0. 02 0. 02 mg/dm 3, 4,,,, ( ),,.,, 6. 3 HPLC, HPLC, 2001 2 6, CHEMTAX 2001 2 6.,,, 36. 14% 79. 27%,.,.
2 : 2001 2 6 233 : [ 1 ] FawleyM W, Lee C M. Pigment composition of the scaly green flagellate M esostigm a viride (M icromonadophyceae) is sim ilar to that of the siphonous green alga B ryopsis plum ose (U lvophyceae) [ J ]. Journal of Phycology, 1990, 26: 6662670. [ 2 ] MackeyM D,Mackey D J, H iggins H W, et al. CHEMTAS2a p rogram for estimating chlass abundances from chem ical markers: app lication to HPLC measurements of phytop lankton[ J ]. Marine Ecology Progress Series, 1996, 144: 2652283. [ 3 ],,. [ J ]., 1999, 124 (4) : 629. [ 4 ] Nelson J R. Rates and possible mechanism of light2dependent degradation of p igments in detritus derived from phytop lankton [ J ]. Journal of M arine Research, 1993, 51: 1552179. [ 5 ],,,. [ J ]., 2003, 22 (3) : 16221. [ 6 ],.. [ J ]., 2000, 22 (3) : 942102. [ 7 ]. [ J ]., 1990, 10 (2) : 1392144. Phytop lankton comm unity structure in we ste rn Xiam en wa te rs from Februa ry to J une 2001 from ana lysis of pho to synthe tic p igm ents HE Xue2jia 1, GAO Ya2hui 2, PENG Xing2yue 2 ( 1. Research Center for Harmful A lgae and Aquatic Environment, J inan University, Guangzhou 510632, China; 2. School of L ife Sciences, Xiamen University, Xiamen 361005, China) Ab s tra c t: Photosynthetic p igments were quantified, using RP2HPLC, from phytop lankton samp les collected in western Xiamen waters from February to June in 2001. D iagnostic p igment data were converted into chlorophyll a biomass values to assess phytop lankton comm unity structure using CHEM TAX software. The effects of environm ental factors on phytop lankton comm unity were also analyzed. emp loyed which consistently reduced the separating tim e by a third. An imp roved RP2HPLC p rotocol was The separation of p igments using the imp roved RP2HPLC p rotocol suggested that the p rime diagnostic p igments in this area during the investigation were Fucoxanthin, 19 2butanoyloxyfucoxanthin, 19 2hexanoyloxyfucoxanthin and peridinin w ith average monthly concentrations varying in the following ranges: 267. 824 655. 076, 20. 118 126. 764, 65. 693 214. 115 and 26. 955 203. 927 g/m 3, respectively. CHEMTAX calculations demonstrated that the dom inant group was diatom s w ith a contribution of 36. 14% to 79. 27% to total phytop lankton biom ass, followed by p rym nesiophytes and dinophytes, which accounted for 4. 50% 37. 40% and 4. 83% 21. 37%, respectively. Total phytop lankton biomass increased from February to June, in response to the continuously increasing temperature. The relative contribution of diatom s varied in a trend opposite to that of p rymnesiophytes, competition was an important factor for phytop lankton community structure in this area. suggesting that inter2species Ke y wo rd s: marine biology; diagnostic p igm ents; phytop lankton community structure; western Xiam en waters DO I: 10. 3969 /J. ISSN. 100028160. 2010. 02. 012 ( : )