DFS Magnetic Sector GC/MS

m a s s s p e c t r o m e t r y DFS Magnetic Sector GC/MS Designed for Highest Sensitivity Analyze Detect Measure Control

DFS MAGNETIC SECTOR GC/MS 2DFS Magnetic Sector GC/MS In continuation of a 50 year history in high performance magnetic sector mass spectrometers, Thermo Electron advances this technology by introducing the DFS double focusing, high resolution magnetic sector. The DFS is a high resolution magnetic sector mass spectrometer like none before. It is the highest performance MS ever built for target compound analysis, and the first completely new double focusing instrument of its class in more than 15 years. The DFS has all the best performance of a magnetic sector MS, but it neither looks nor feels like one.

The DFS delivers Highest sensitivity Highest signal/noise Lowest limits of detection Lowest limits of quantitation Highest specificity Highest sample throughput Unattended automatic operation Running the DFS is as simple and straightforward as operating a low resolution benchtop MS. Automatic tuning Automatic universal mass calibration Intuitive user interface Intuitive method and sequence set-up Comprehensive and flexible data evaluation and reporting The DFS is friendly Fits into a small laboratory Uses less power Quieter Requires little installation time Immediately productive.and it is versatile Highest sensitivity EI/CI Positive and negative ions Solids probe FAB...and other ionization and inlet techniques The DFS neither looks nor feels like a traditional magnetic sector MS. It defines a new class of high performance mass spectrometers. 3

DFS Magnetic Sector GC/MS EXPERIENCE THE NEW DIMENSION The revolutionary ion optics and the intuitive user interface make operation of the instrument easy and straightforward. Technology Several new technologies have been incorporated in this revolutionary mass spectrometer. All these combined, provide the most powerful GC/MS mass spectrometer ever designed. Novel Analyzer The newly designed analyzer is the first with virtually no image aberrations. It is perfectly double focusing, employing an ultra high precision toroidal electrostatic analyzer and a carefully refined magnetic analyzer. This directly translates into stability and ruggedness Tuning for highest sensitivity does not influence the preset resolution Fully automatic ion source tuning Automatic setting of mass resolution No tuning of other ion optical elements Mass calibration completely independent from ion Single universal mass calibration for all modes of operation The DFS is forgiving and robust while delivering the highest sensitivity and specificity. Ion Source The ion source has been designed with special emphasis on sensitivity, durability and ease of maintenance. Low maintenance and short downtime increase productivity. Exchange of ion volume and filament without venting Plug-in ion source for easy maintenance Optimized long lifetime filaments for EI and CI New easy-to-use high stability reference inlet system Electronics The DFS has completely new, state-of-the-art electronics. It uses dedicated micro-controllers on all boards, enabling efficient and fast computer control and read-back of all important parameters and voltages through a common interconnect bus. It is verified to be completely immune against high voltage arcing another contribution to robustness and reliability. The magnetic field regulator is fast and stable, yet very energy efficient, utilizing power-on-demand switching power supplies. It fully complies with the EU power factor correction directive. The magnetic field is controlled with 20 bit digital resolution and receives its feedback from a unique magnetic field probe, supporting the single universal mass calibration. Chassis and Cabinet The new small-footprint cabinet is aesthetic and functional. It contains the analyzer, the electronics, and the complete pumping system, including roughing pumps. No precious space is wasted. Through its ingenious design it allows the DFS to be shipped completely assembled with the magnet in place. This means very short installation time, no need for a crane, and the security that the system arrives exactly the way it has been tested in the factory. Due to the innovative technology the mass spectrometer weights less than conventional magnetic sector instruments, thus minimizing the demands on the laboratory environment. Tune Software TunePlus is the renowned user interface for ion trap and triple quadrupole MS (Finnigan LCQ, LTQ, LXQ, TSQ Quantum ). For the first time, a magnetic sector mass spectrometer uses the same concept, making tuning, experiment and sequence set-up intuitive and simple. High resolution MID is an integral part of the user interface. GC/MS Productivity The DFS delivers unprecedented flexibility and productivity for large sample series. It can be optionally equipped with two Trace GC Ultra units. The two GC units are simultaneously installed at the same ion source for separation on two independent GC setups. It is a system designed for unattended operation, in which the TriPlus autosampler offers highest sample capacity and ultimate sampling flexibility while serving one or two Trace GC units from shared sample trays. GCs can be equipped with a wide range of injectors, from cold on-column and split/splitless injectors, going to advanced temperature programmed injectors (PTV), utilizing latest developments in large volume injection techniques. 4

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DFS HIGH PERFORMANCE MAGNETIC SECTOR GC/MS With its innovative ion optics and revolutionary design, the DFS is the new gold standard in POP analysis. 6

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8DFS Magnetic Sector GC/MS

UNPRECEDENTED TARGET COMPOUND PERFORMANCE Pesticides The routine confirmation and quantitation of pesticides on the DFS high resolution GC/MS is demonstrated from a complex biological matrix sample extract measured in high resolution negative CI mode. More than 40 compounds from the target list, representing various pesticides and herbicides from multiple chemical compound classes, are monitored using high resolution MID and unambiguously identified on the retention time combined with the accurate mass information. Excellent sensitivity and quantitative linearity over a wide concentration range down to the low femtogram level is shown in Figure 1 and 2 for Tolylfluanid, a phenylsulfamide fungicide. Figure 1 presents a measurement of ca. 25 fg, analyzed directly from a raw sample extract. Very little matrix interference is observed. Figure 2 shows the excellent linearity of the method over the investigated concentration range. Dioxins and Furans The new DFS high resolution GC/MS is the reference instrument for the analysis on polybrominated and polychlorinated dioxins and furans (PCDD/F), delivering reliable results on the lowest detection levels ever achieved, withstanding any legal interrogation. Figure 3 presents a typical measurement of a food sample containing low concentrations of 2,3,7,8-substituted PCDD/F congeners. The chromatogram presented here on 2,3,7,8- tetrachloro dibenzo-p-dioxin corresponds to an approximate level of 20 fg of the substance injected on-column. Depending on the matrix interferences, detection levels in the single digit femtogram range can be achieved. Relative Abundance 100 95 90 85 80 75 70 65 60 55 50 45 40 21.09 35 30 21.02 25 RT: 21.21 AA: 2258 21.70 21.53 21.55 21.40 21.47 21.32 21.80 22.04 21.88 21.98 21.0 21.2 21.4 21.6 21.8 22.0 Time (min) 0.120 0.115 0.110 0.105 0.100 0.095 0.090 0.085 0.080 0.075 Tolylfluanid 0.070 0.065 R 2 =0.9997 0.060 0.055 0.050 0.045 0.040 0.035 0.030 0.025 0.020 0.015 0.010 0.005 0.000 0 1 2 3 4 5 6 7 8 9 10 11 12 13 Area Ratio Relative Abundance m/z 212.06 RT: 12.89 AA: 927 100 90 80 70 60 50 40 30 20 11.90 11.33 11.51 12.11 12.33 12.60 13.33 13.67 13.12 13.92 10 13.39 0 100 RT: 12.89 AA: 1117 90 80 70 60 50 40 30 20 12.99 11.91 12.28 12.79 11.40 10 12.36 13.21 13.75 13.29 11.27 11.58 12.00 13.70 13.99 0 11.5 12.0 12.5 13.0 13.5 14.0 Time (min) weighting: equal m/z 319.897 m/z 321.894 Figure 1: High resolution MID chromatogram for 25 fg Tolylfluanid injected on-column in NCI mode Figure 2: Calibration curve for Tolylfluanid in NCI mode Figure 3: High resolution MID chromatogram of TCDD in a typical food sample 9

DFS Magnetic Sector GC/MS THE DFS REDEFINES PERFORMANCE The innovative analyzer design provides the sensitivity to routinely analyze compounds in the low femtogram range. Performance The DFS redefines performance. The innovative analyzer design provides the sensitivity to routinely analyze compounds in the low femtogram range. 100 fg of 2,3,7,8-tetrachloro dibenzo-p-dioxin injected on-column will give a signal-to-noise ratio of better than 800:1 on unsmoothed data, when measured in high resolution lock mode MID. The unprecedented performance of the DFS will help you to gain the largest amount of data from precious samples and lets you cut down on the amount of sample to extract. This is especially important in the ultra trace analysis of lipophilic POPs. The cleanup for these compounds is a lengthy and cost-intensive process employing several chromatographic steps. In cases where sensitivity is not sufficient, a higher amount of sample needs to be cleaned up, which in certain cases can only be accomplished by parallelization of the process. The sensitivity of DFS allows the lowest amount of sample use for quick cleanup. DFS Features Mass Range of 2-6000 Da Scan speeds continuously variable from 0.1-10,000 sec/decade Radially laminated magnet Field based calibration, i.e. mass calibration is independent of scan speed, polarity, ionization mode High performance toroidal ESA Detection system with ± 20kV conversion dynode and long-life secondary electron multiplier for efficient detection of ions Full range of ionization modes available: EI, CI, FAB, ESI, APCI, FD/FI Pneumatically actuated exchange lock for ventless exchange of ionization volumes Water cooled DI probe and DCI probe available Ultra low detection limits: 100 fg TCDD on-column in high resolution MID mode gives S/N > 800:1 Dual Trace GC Ultra configuration with one TriPlus XT autosampler allows GC columns of different polarity used in one sequence. Samples can be injected from one vial into two different GCs in fully automated fashion Removable ion volumes for easy source maintenance 10

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DFS Magnetic Sector GC/MS XCALIBUR A UNIFIED PLATFORM FOR MS One intuitive platform for GC/MS, LC/MS, and Advanced MS instruments provides confident control from method development to reporting. Xcalibur Platform Xcalibur is the most powerful data system available today, delivering a unique combination of functionality, system control, and ease of use. The software is designed to guide you through your daily analytical tasks. This powerful simplicity combined with the advanced features of the Microsoft Windows operating system and Microsoft Office productivity tools provides an analytical platform that s second to none. The DFS generates high quality data regardless of matrix and concentration, low or high resolution, scanning or MID data. The enormous amount of information hidden in the data can be revealed using the built-in features of Xcalibur. Xcalibur provides complete control of the DFS, the Finnigan Trace GC Ultra, and the TriPlus autosampler, as well as other devices. Xcalibur contains a built-in audit trail to ensure compliance with your laboratory s SOPs and quality programs. Generating reports from your data has never been easier than with XReport, Xcalibur s new reporting application. Designed with ease of use in mind, XReport provides a flexible visual interface for intuitive report template creation and report generation. Simple drop-down menus and drag-and-drop objects provide complete flexibility for your reporting needs. Integrates layered applications, including Mass Frontier Simplifies interpretation of mass spectra with improved predictive fragmentation tools, and user friendly classification modules. EnviroLab Forms Automated reporting forms package designed for the regulated environmental laboratory. Installation Requirements 12 Power Temperature Humidity Cooling Water Helium CI Gases Compressed Air Pump Exhaust 3-phase, 230/400V ± 10% 50/60 Hz, fused 25A per phase 15-29 C (59-79 F) Air conditioning with better than 2 C stability recommended 30-70%, non-condensing, non-corrosive ca. 13 L/min, Temperature 20-25 C 3-5 bar (43-65 psi) For GC carrier gas: 99.996% or better, ultra-high purity. Total hydrocarbons should be less than 2.0 ppm Methane, isobutane, or ammonia; 99.8% or better For operation of the pneumatic valves: 6-8 bar, filtered for particulate matter, oil mist and moisture 25 mm, 1 L/min

TARGETQUAN The dedicated quantitation package is specifically designed for the isotope dilution technique, covering protocols like EPA 1613, JIS K 0311/0312, or EN 1948. Dedicated Quantitation Package TargetQuan, a dedicated quantitation package specifically designed for isotope dilution quantitation is available as part of Xcalibur. It has been tailored to the EPA, EN, and JIS protocols for analysis of persistent organic pollutants, such as halogenated dioxins and furans, polychlorinated biphenyls, brominated flame retardants, and many more. The software package consists of three individual programs, QuanDesk, Response, and Reporter. QuanDesk is the main quantitation application, which includes the user interface for method setup, peak integration, and result viewing. Response deals with calibration curves, while Reporter helps to created printed or electronic chromatogram documents. Features Quantitation based on relative response factors (RRF) User definable dependency of standards One quantitation mass and up to two ratio masses for confirmation based on isotopic abundance Freely configurable spreadsheet for data viewing and method setup Automated or manual peak integration Retention time correction Toxicity equivalents (TEQ) according to WHO definition including lower, medium, and upper boundary calculation User definable summation of calculated amounts or TEQs for reporting of sum TEQ values Allows quantitation based on average response of selected compound (for EPA 1613) Ready To Go The software comes with templates for quantitation of polychlorinated dioxins, furans (EPA 1613 and others), and other POPs. Automation Built-in scripting allows for full automation from creating a calibration curve to measuring samples unattended. The simple scripting functionality enables you to tailor the software to follow the workflow of your laboratory. Reporting Spreadsheets can be printed, saved, exported, or emailed directly from the QuanDesk application. The look of these spreadsheets are user definable, such that columns (representing values), rows (representing individual compounds), headings, and display settings can be arranged in any desired arrangement, and can be saved as layout files for easy access. The Reporter application comes with an intuitive editor for creating reporting templates using drag-and-drop objects. Using the templates, chromatogram reports can be either printed or stored as document files automatically from a sequence. LIMS Export Any selected set of parameters and compound can be exported to a LIMS system using CSV, ASCII, or XLS file export. File export can be initiated automatically through a sequence, or manually. 1251 1611 2091 1611 1251 Single GC configuration Ø 780 322.5 585 1567.5 Dual GC configuration Ø 780 322.5 585 1567.5 099 099 13 270 870 870 870

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