Soybean Insecticide Screening Test 1 Field north of TRIA house Beaumont, TX 2014 I II III IV

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North Soybean Insecticide Screening Test 1 Field north of TRIA house Beaumont, TX 2014 PLOT PLAN I II III IV 1 7 (W) 8 3 (Y) 15 6 (O) 22 4 (P) 2 6 (O) 9 1 (B) 16 5 (LG) 23 7 (W) 3 5 (LG) 10 2 (R) 17 7 (W) 24 1 (B) 4 4 (P) 11 4 (P) 18 1 (B) 25 2 (R) 5 3 (Y) 12 6 (O) 19 2 (R) 26 3 (Y) 6 1 (B) 13 7 (W) 20 4 (P) 27 5 (LG) 7 2 (R) 14 5 (LG) 21 3 (Y) 28 6 (O) Plot size: 4 rows, 30 inch row spacing, 30 ft long Variety: AG6732 Note: smaller numbers in italics are plot numbers TREATMENT DESCRIPTIO, RATES AND TIMINGS Treatment. no. Flag color Description 1 Blue Untreated --- 2 Red Belt SC 2 3 Yellow Belt SC 2.5 4 Pink Belt SC 3 5 Light green Besiege SC 7 6 Orange Prevathon SC 14 7 White Karate Z 2 Agronomic and Cultural Information Experimental design: Randomized complete block with 7 treatments and 4 replications Planting: Planted test (AG6732) on Jul 30 (60 lb/a) Plot size = 4 rows, 30 inch row spacing, 40 ft long Emergence on Aug 6 66

Herbicide: Irrigation: Treatments: First @ 0.75 oz/a and Dual Magnum @ 2.5 pt/a were applied preemergence with a 2-person hand-held spray boom (13- No. 2 cone nozzles, 50 mesh screens, 15 gpa final spray volume) on Jul 31. Plots were not irrigated---rainfed only Treatments 2 7 applied with a hand-held spray boom (2 nozzle boom (Conejet TSS cone nozzles, 50 mesh screens, 22 gpa final spray volume) on Sep 30 Sampling: Soybeans @ R5 on Sep 30, Oct 1 and Oct 3, and R6 on Oct 9 and Oct 13 Insect sweeps (12 sweeps/plot) on Oct 1, Oct 3, Oct 9 and Oct 13 Discussion One day after treatment (DAT), none of the treatments significantly reduced (compared to the untreated) Lepidoptera populations composed mainly of green cloverworm (GCW) (Table 1). Also, populations of stink bugs were too low for meaningful comparisons (Table 2). However, threecornered alfalfa hoppers (TCAH) were significantly less than the untreated in Besiege SC and Karate Z plots (Table 3). Banded cucumber beetle (BCB) and spider populations were too low for meaningful comparisons. Three DAT, total Lepidoptera populations in the untreated were low, but all treatments significantly reduced these populations (Table 4). Stink bug populations were too low for meaningful comparisons (Table 5). TCAH populations were significantly less in Besiege SC and Karate Z plots (Table 6). Again, spider populations were too low for meaningful comparisons (Table 6). Nine DAT, Lepidoptera and stink bug populations were too low for meaningful evaluation (Tables 7 and 8). TCAH populations were increasing and only Besiege SC and Karate Z provided control (Table 9). Besiege SC and Karate Z also controlled BCB (Table 9). Thirteen DAT, Lepidoptera populations were again too low for meaningful evaluation (Table 10). Redbanded stink bug (RBSB) populations were increasing and only Besiege SC and Karate Z provided good control (Table 11). TCAH and BCB populations also were controlled by Besiege Sc and Karate Z (Table 12.). Plots were not harvested because of the late planting date--- July 30. Plots were not harvested due to the late planting date. In conclusion, the Besiege SC and Karate Z treatments gave the best control of a broad spectrum of pest insects. Unfortunately, Lepidoptera populations were not as high as desired. 67

Table 1. Mean lepidopterous larvae data in 12 sweeps on Oct 1 (1 DAT) for soybean insecticide screening test. Beaumont, TX. 2014. Treatment Soybean looper Green cloverworm Velvetbean caterpillar Total leps Untreated --- 0.3 4.3 0.5 5.0 Belt SC 2 0 2.0 1.0 3.0 Belt SC 2.5 0 1.3 1.0 2.3 Belt SC 3 0.3 1.8 0.5 2.5 Besiege SC 7 0 1.0 0.3 1.3 Prevathon SC 14 0.5 1.0 0.5 2.0 Karate Z 2 0 1.0 0.5 1.5 Means in a column are not significantly different (P = Table 2. Mean stink bug data in 12 sweeps on Oct 1 (1 DAT) for soybean insecticide screening test. Beaumont, TX. 2014. Redbanded stink bug Brown stink Total stink Treatment Adults Nymphs Total bug adults bugs Untreated --- 0.5 0 0.5 0.3 0.8 Belt SC 2 0.8 0.3 1.0 0.3 1.3 Belt SC 2.5 1.5 0.3 1.8 0.3 2.0 Belt SC 3 0.8 0 0.8 0 0.8 Besiege SC 7 0.8 0 0.8 0 0.8 Prevathon SC 14 0.3 0 0.3 0 0.3 Karate Z 2 0.5 0 0.5 0 0.5 Means in a column are not significantly different (P = 68

Table 3. Mean threecornered alfalfa hopper and miscellaneous insect data in 12 sweeps on Oct 1 (1 DAT) for soybean insecticide screening test. Beaumont, TX. 2014. Banded Threecornered alfalfa hopper cucumber Treatment Adults Nymphs Total beetle Spiders Untreated --- 6.5 ab 0.5 7.0 a 0.8 ab 1.5 ab Belt SC 2 7.0 a 0.3 7.3 a 1.3 a 0.8 abc Belt SC 2.5 3.0 b 1.0 4.0 ab 0.5 ab 2.0 a Belt SC 3 3.8 ab 0.3 4.0 ab 1.8 a 1.8 a Besiege SC 7 0.3 c 0.3 0.5 c 0 b 0.8 abc Prevathon SC 14 6.3 ab 0.8 7.0 a 1.0 ab 0.3 bc Karate Z 2 0.5 c 0.8 1.3 bc 0 b 0 c Means in a column followed by the same or no letter are not significantly () different (P = Table 4. Mean lepidopterous larvae data in 12 sweeps on Oct 3 (3 DAT) for soybean insecticide screening test. Beaumont, TX. 2014. Treatment Soybean looper Green cloverworm Velvetbean caterpillar Total leps Untreated --- 0.3 2.8 a 0.8 a 3.8 a Belt SC 2 0 0.3 b 0 b 0.3 b Belt SC 2.5 0 0 b 0 b 0 b Belt SC 3 0 0 b 0 b 0 b Besiege SC 7 0 0 b 0 b 0 b Prevathon SC 14 0 0.3 b 0 b 0.3 b Karate Z 2 0 0.8 b 0 b 0.8 b Means in a column followed by the same or no letter are not significantly () different (P = 69

Table 5. Mean stink bug data in 12 sweeps on Oct 3 (3 DAT) for soybean insecticide screening test. Beaumont, TX. 2014. Redbanded stink bug Brown stink Total stink Treatment Adults Nymphs Total bug adults bugs Untreated --- 0.3 0.8 1.0 0.3 1.3 Belt SC 2 0.8 0.8 1.5 0 1.5 Belt SC 2.5 0.8 0.8 1.5 0 1.5 Belt SC 3 0.8 0 0.8 0 0.8 Besiege SC 7 0.5 0 0.5 0 0.5 Prevathon SC 14 1.3 0.3 1.5 0.3 1.8 Karate Z 2 0.8 0 0.8 0 0.8 Means in a column are not significantly different (P = Table 6. Mean threecornered alfalfa hopper and miscellaneous insect data in 12 sweeps on Oct 3 (3 DAT) for soybean insecticide screening test. Beaumont, TX. 2014. Threecornered alfalfa hopper Treatment Adults Nymphs Total Spiders Untreated --- 4.5 b 0.5 5.0 b 1.0 ab Belt SC 2 10.0 a 0 10.0 a 0.3 b Belt SC 2.5 8.3 ab 0.3 8.5 ab 1.0 ab Belt SC 3 6.5 ab 0 6.5 ab 2.5 a Besiege SC 7 1.0 c 0.3 1.3 c 0.3 b Prevathon SC 14 6.5 ab 0.8 7.3 ab 2.0 a Karate Z 2 0.3 c 0.3 0.5 c 1.3 ab Means in a column followed by the same or no letter are not significantly () different (P = 70

Table 7. Mean lepidopterous larvae data in 12 sweeps on Oct 9 (9 DAT) for soybean insecticide screening test. Beaumont, TX. 2014. Treatment Soybean looper Green cloverworm Velvetbean caterpillar Total leps Untreated --- 0 1.0 a 0.8 a 1.8 a Belt SC 2 0 0 b 0 b 0 b Belt SC 2.5 0 0 b 0 b 0 b Belt SC 3 0 0 b 0 b 0 b Besiege SC 7 0 0 b 0 b 0 b Prevathon SC 14 0 0 b 0 b 0 b Karate Z 2 0.3 0 b 0 b 0.3 b Means in a column followed by the same or no letter are not significantly () different (P = Table 8. Mean stink bug data in 12 sweeps on Oct 9 (9 DAT) for soybean insecticide screening test. Beaumont, TX. 2014. Redbanded stink bug Brown stink bug Total Treatment Adults Nymphs Total Adults Nymphs Total stink bugs Untreated --- 0.3 2.5 2.8 0 0 0 2.8 Belt SC 2 0.8 0.5 1.3 0 0 0 1.3 Belt SC 2.5 0.8 3.0 3.8 0 0 0 3.8 Belt SC 3 0 2.5 2.5 0 0 0 2.5 Besiege SC 7 1.3 0 1.3 0 0 0 1.3 Prevathon SC 14 0.3 2.0 2.3 0 0.3 0.3 2.5 Karate Z 2 0.8 0.3 1.0 0.3 0 0.3 1.3 Means in a column are not significantly different (P = 71

Table 9. Mean threecornered alfalfa hopper and miscellaneous insect data in 12 sweeps on Oct 9 (9 DAT) for soybean insecticide screening test. Beaumont, TX. 2014. Banded Threecornered alfalfa hopper cucumber Treatment Adults Nymphs Total beetle Untreated --- 10.0 b 0 10.0 c 2.8 ab Belt SC 2 17.3 ab 0.3 17.5 abc 2.3 ab Belt SC 2.5 10.3 ab 0 10.3 bc 1.5 bc Belt SC 3 18.0 a 0.3 18.3 ab 3.5 ab Besiege SC 7 3.5 c 0 3.5 d 0.3 c Prevathon SC 14 18.5 a 0.3 18.8 a 4.3 a Karate Z 2 2.5 c 0 2.5 d 0.3 c Means in a column followed by the same or no letter are not significantly () different (P = Table 10. Mean lepidopterous larvae data in 12 sweeps on Oct 13 (13 DAT) for soybean insecticide screening test. Beaumont, TX. 2014. Treatment Soybean looper Green cloverworm Velvetbean caterpillar Total leps Untreated --- 0.3 1.0 a 0.3 1.5 a Belt SC 2 0 0 b 0 0 b Belt SC 2.5 0 0 b 0 0 b Belt SC 3 0 0 b 0 0 b Besiege SC 7 0 0 b 0 0 b Prevathon SC 14 0 0 b 0 0 b Karate Z 2 0 0 b 0 0 b Means in a column followed by the same or no letter are not significantly () different (P = 72

Table 11. Mean stink bug data in 12 sweeps on Oct 13 (13 DAT) for soybean insecticide screening test. Beaumont, TX. 2014. Southern Redbanded stink bug green stink Total stink Treatment bug nymphs Adults Nymphs Total bugs Untreated --- 0.5 1.0 6.8 a 7.8 a 8.3 a Belt SC 2 0 0.3 3.0 ab 3.3 ab 3.3 bc Belt SC 2.5 0 0 5.8 a 5.8 a 5.8 ab Belt SC 3 0 0.5 7.3 a 7.8 a 7.8 ab Besiege SC 7 0 0 0 c 0 c 0 d Prevathon SC 14 0 1.3 4.5 a 5.8 a 5.8 ab Karate Z 2 0 1.0 0.3 bc 1.3 bc 1.3 cd Means in a column followed by the same or no letter are not significantly () different (P = 0.05, ANOVA and LSD). Table 12. Mean threecornered alfalfa hopper and miscellaneous insect data in 12 sweeps on Oct 13 (13 DAT) for soybean insecticide screening test. Beaumont, TX. 2014. Banded Threecornered alfalfa hopper cucumber Treatment Adults Nymphs Total beetle Untreated --- 6.5 ab 0 6.5 a 3.3 a Belt SC 2 4.3 bc 0.3 4.5 a 1.8 ab Belt SC 2.5 4.3 ab 0 4.3 ab 2.0 ab Belt SC 3 8.3 a 0 8.3 a 2.0 ab Besiege SC 7 1.5 d 0 1.5 c 0.3 b Prevathon SC 14 7.5 ab 0 7.5 a 1.8 ab Karate Z 2 1.8 cd 0 1.8 bc 0.3 b Means in a column followed by the same or no letter are not significantly () different (P = 73