1999 1 ENV IRONM EN TAL SC IEN CE Jan, 1999 3 (, 361005 E2m ail: kelqb@ jingxian xm u edu cn) (, 361005), (P hanerochaete ch ry sosp orium ) Pb 2+, ph 415, 10 6 gm l, 80 011%, 150rgm in, 39 3d, 115 117mm,,, Pb 2+ 012mo lgl N aoh, 25m ggl 95%, Pb 2+,,, Pb 2+,,,, The Effects of Physica l and Chem ica l Cond ition s on Form ing M ycelia l Pellet of P hanerochaete ch rysosp orium and B iosorption of L ead L i Q ingb iao W u Juan Yang Hongquan D eng Xu L u Y inghua (D epartm ent of Chem ical Engineering, X iam en U niversity, X iam en 361005 E2m ail: kelqb@ jingxian xm u edu cn) Hong L iyu Cai L izhe (Research Center of Environm ent and Science, X iam en U niversity, X iam en 361005) Abstract T he research on the grow th of P hanerochaete ch ry sosp orium in the fo rm of pellet w as m ade in th is paper to exam ine the effects of physical and chem ical condition s on the fo rm ation of m ycelial pelletṡ T he experim en tal resu lts show ed that the m icroo rgan ism pellets of sm oo th su rface, even size (diam eter) and good m echan ical p roperties cou ld be ob tained at the fo llw o ing cu ltu re condition s: ph 415, 10 6 spo resgm l in inocu lum, 011% Tw een 80 in m edium, h igh CgN and on the shaker of 150rgm in fo r 3d U sing P ch ry sosp orium m ycelial pellets in the b io so rp tion of Pb 2+ from aqueou s so lu tion, the up take reached the m ax im um w hen the diam eter of pellet w as in the range of 115 117mm W hen the pellets w ere p retreated by bo iling in 012m o lgl N aoh fo r 40m in, the Pb 2+ up take w as far h igher than the pellets w ithou t p retreatm en t, and the rem ova l efficiency cou ld reach above 95% T he resea rch resu lt s show ed tha t the rem ova l of Pb 2+ from aqueou s so lu tion by P ch ry sosp orium m ycelial pellets w as p ractical Keywords P hanerochaete ch ry sosp orium, m ycelial pellet, b io so rp tion, Pb 2+, CgN, b iom ass, spo re su spen sion, su rface2active agen ṫ L inko [ 2 ] [ 1-3 ], ( P ch ry sosp orium ) [ 4, ],, Knapp [ 5 ] Sharm a [ 1 ] 3 :, 35,, (A sp erg illus p a rasiticus) : 1998204206 1994-2007 China Academic Journal Electronic Publishing House All rights reserved http://wwwcnkinet
34 20 (O range g ),, 98% (2) (A sp erg illus f lavus) [ 6 ],, 1 1 (S accha rom y ces cerev isiae) [ 7 ], 24h, (Z oog loea ram ig era) [ 8 ] 50m ggl 100m l, 012m o lgl Engl [ 7 ] 015m o lgl ph ( 415), 012g ( ) ( ), 26 16h,, PE3030 (, Perk in2e lm er ) Pb 2+,,, (3) 012m o lgl N aoh 40m in,,, 1 111 (1) (m g g) (P hanerochaete ch ry sosp orium ),, ( ( 1L ) : Basal g ) m edium 100m l, 10g, (011m o lgl ) 100m l, 01001g, N H 4H 2PO 4 0125g, 80 ( 011% ) 50m l, 211 60m l, 100m l Basal g m edium (CgN = 131, ph 415) (1L ) : KH 2PO 4 2g,M gso 4 7H 2O 5g, CaC l2, 10% 9 10 5 gm l 1g, 100m l,, ( 2 ) 150rgm in, 39 1 012500g, 100m l, 1+ 1 1 20m l,, 1L, 250m ggl 112 (1), ph S23C ph, 2 gh gmm 11 19 0180 0195 26, 0190 1110 36, 1130 1140 48,, 1140 1150 60, 1160 1165 121 20m in, 72,,, 1160 1170, 80 1160 1170, 1,, 10%,, 3d :, GQ 70E2,,, ; 1994-2007 China Academic Journal Electronic Publishing House All rights reserved http://wwwcnkinet
1 35 116 117mm,, 72h 212 ph,, ph,, 2 ph 2, 1 1 11 21Pb 2+ ph 415 615 (117 119ggL ), ph 7, ph 2, ph 415 615,, ph 7,,,,,,, ph ph 1,,, 19 60h, ;,, 72h ph, 48h, [ 9 ] ph, 72h Pb 2+, 16107m g g,, ph 415 615 ph gmm 2 ph Pb 2+ 1) g L - 1 Pb 2+ gm g g - 1 210 010663 01342 5178 315 010323 118 119 11572, 9110 415 010370 116 117 11837, 16134 515 010375 116 117 11797, 16101 615 010380 116 117 11668, 15185 715 010386 114 115 11581 13182 815 010396 112 113 11494 12158 915 010444 019 110 11224 11181 1015 010671 018 019 01978 9161 1) : = 9 10 5 gm l, CgN = 131, 39, 150rgm in, 3d Pb 2+, ph 16134m g g 66164%, 213 116 117mm,,,,,,,, ph 415 1994-2007 China Academic Journal Electronic Publishing House All rights reserved http://wwwcnkinet
36 20 3 CgN Pb 2+ 1) CgN g L - 1 N H 4H 2PO 4 g L - 1 g L - 1 gmm Pb 2+ gm g g - 1 4110 10 00 8 00 3 148 2 0 2 1, 15106 25126 10 0 1 30 2 847 1 9 2 0, 15 47 65168 5 00 0 25 1 717 1 8 1 9, 16138 98163 7150 0 25 1 810 1 8 1 9, 16195 131135 10 00 0 25 1 927 1 6 1 7, 17118 1) : ph 415, = 211 10 6 gm l, 39, 150rgm in, 3d 3,,,,,, 100,, CgN 131 (116 117mm ), Pb 2+,,, 214,,, 80,,, 4 Pb 2+ 1) Tw een80 g% 0 0 05 0 1 0 2 0 3 0 5 1 0 1 5 2 0 gmm 1 2-1 5 1 6-1 7 1 6-1 7 1 8 1 9 1 7-1 8 117 118 1 5-1 6 1 4-1 5 1 4-1 5 g L - 1 1 656 2 133 2 269 2 282 2 303 21389 2 412 2 436 2 501 g% 57 22 65 00 65 65 58 57 59 79 60 57 65 47 57 65 56 19 gm g g - 1 14 31 16 25 16 41 14 64 14 95 15 14 16 37 14 41 14 05 1) : ph 415, = 8 1 10 5 gm l, CgN = 131, 39, 150rgm in, 3d ( 4), 80 0,, 2%,, 115 117mm,, 16141m g g, 65165% 80,, ; 215 80,,, 80 ( 5),,,, 80,,, 20 60,,, 80,,,,,, 4 2, 1994-2007 China Academic Journal Electronic Publishing House All rights reserved http://wwwcnkinet
1 37,, 216, 6, 3, 10 6 gm l, 5 Pb 2+ 1) g m l - 1 g L - 1 gmm g% 10 6 2 045 0 0391 1 6-1 7 65 89, 10 5 1 717 0 0326 2 4-2 5 33 86, 10 4 1 197 0 0302 3 5-3 6 32 67, 10 3 0 477 0 0284 4 5-5 0 28 61, 1) : ph 4 5, CgN = 131, 39, 150rgm in, 3d 1) 6 gr m in - 1 g L - 1 gmm 108 01410 010276 510 610,, 132 11833 010354 119 210,, 150 11894 010385 117 118,, 1) : ph 4 5, = 112 10 6 gm l, CgN = 131, 39, 3d 6, ( CO 2),, (150rgm in) ;,, 217 Ca 2+,,,, Ca 2+ ( 7), [ 10 ] CaC l2,,,, CaC l2 0104%, ;, CaC l2, 0105%, CaC l2, 0101% 011%,,, Ca 2+,,, ; 25m ggl,,, Ca 2+, Pb 2+ (, ),,, Pb 2+, 65% 1994-2007 China Academic Journal Electronic Publishing House All rights reserved http://wwwcnkinet
38 20 2) 7 CaCl2 CaC l2 g% 0101 0 02 0 04 0 05 0 10 gmm 1 5-1 6 1 6-1 7 1 7-1 8 1 6-1 7 1 7-1 8 g L - 1 1 811 1 888 2 319 1 781 1 762 0 0323 0 0331 0 0361 0 0423 0 0457 gm g g - 1 11 91 11 79 11 65 11 52 11 41 g% 95 25 94 29 93 19 92 14 91 22 1) : ph 4 5, = 1 4 10 6 gm l, CgN = 131, 39, 150rgm in, 3d 90%,,,, CaC l2 0104%,,, Pb 2+ 3 ( 1) ph, ph 131, ph 415, 80 011%, 10 6 gm l, 150rgm in 3d, (2), (3),,, of pellets A 1 Sharm a A, Padw al2d esai S R O n the relationsh ip be2 tw een pellet size and aflatoxin yield in A sp erg illus p ara2 siticus B io techno l and B ioeng, 1985, 27 ( 11): 1577 1580 2 L inko S P roduction and characterization of extracellular lignin peroxidase from immobilized P hanerochaete chry sosp orium in a 10L bio reacto ṙ Enzym e M icrob T echno l, 1988, 10 (6) : 410 417 3,, 1 CEH 1, 1996, 16 (3) : 25 26 4 P ro sser J I, Tough A J Grow th m echanism s and grow th kinetics of filam entous m icroo rganism B io techno logy, 1991, 10 (4) : 253 274 5 Knapp J S, Fu2m ing Zhang, T ap ley K N D eco lourisation of O range g by a wood2ro tting fungus J Chem T ech B io techno l, 1997, 69 (3) : 289 296 6 H afez N, A bdel2razek A S, H afez M B A ccum ulation of som e heavy m etals on A sp erg illus f lavuṡ J Chem T ech B io techno l, 1997, 68 (1) : 19 22 7 Engl A, Kunz B B io so rp tion of heavy m etals by S accha2 romy ces cerev isiae: effects of nutrient conditions J Chem T ech B io techno l, 1995, 63 (3) : 257 261 8 N o rberg A, Persson H A ccum ulation of heavy m etal ions by Z oog loea ram ig era B io techno l and B ioeng, 1984, 26 (3) : 239 246 9 M etz B, Ko ssen N W F T he grow th of mo lds in the fo rm eng, 1997, 110 (6) : 781 799 literature review B io techno l and B io2 10 1 1 :, 1995 114 1994-2007 China Academic Journal Electronic Publishing House All rights reserved http://wwwcnkinet