Analytical Reference Materials Delta-9-THC Standard Catalog # 3467 Lot # A82745 11 Benner Circle Bellefonte, PA 16823-8812 (814) 353-13 FOR LABORATORY USE ONLY. READ MSDS PRIOR TO USE. RAW MATERIAL TEST INFORMATION AVAILABLE UPON REQUEST MANUFACTURED UNDER RESTEK'S ISO 91 REGISTERED QUALITY SYSTEM Page 1 of 11
11 Benner Circle Bellefonte, PA 16823-8812 Tel: (8)356-1688 Fax: (814)353-139 www.restek.com Certificate of Analysis FOR LABORATORY USE ONLY-READ MSDS PRIOR TO USE. Catalog No. : 3467 Lot No.: A82745 Description : Delta-9-THC Standard Delta-9-THC Standard, 1,µg/ml, Methanol, 1ml/ampule Container Size : 5 2 ml Pkg Amt: 5 > 1 ml Expiration Date : July 213 Storage: 1 C or colder Elution Order Compound CAS # Percent Purity 2 Grav. Conc. (weight/volume) 3 Grav.Uncert. (95% C.L.; K=2) 1 delta-9-tetrahydrocannabinol 1972-8-3 93% 1. mg/ml +/- <.1 mg/ml 4 Solvent: Methanol 67-56-1 99% Column: 3m x.25mm x.25um Rtx-5 (cat.#1223) Carrier Gas: hydrogen-constant pressure 1 psi. Temp. Program: 75 C (hold 1 min.) to 33 C @ 2 C/min. (hold 5 min.) Inj. Temp: 2 C Det. Temp: 33 C Det. Type: FID Page 2 of 11
Date Passed: 6-Jul-211 Balance: 11283695 1 Expiration date of the unopened ampule stored at the recommended storage condition is the last day of the month listed. 2A Purity and chemical identity are determined by one or more of the following techniques: GC/FID, HPLC, GC/ECD, GC/MS. Purity value is rounded to the nearest whole number. See data pack or contact Restek for further details. 2B Compounds with a listed purity of less than 99% have been weight corrected to compensate for impurities. 2C The following types of compounds will have a listed purity of less than 99%: Aldehyde/Ketone-DNPH compounds, Bromides, Chlorides, HCL salts, HBR salts, sulfates, hydrates, and other compounds as necessary. The listed purity is a correction factor that is equivalent to the percentage of parent compound in the molecule. This correction factor is used to calculate the amount of compound necessary to achieve the desired concentration of the parent compound in solution. The concentration listed on the certificate is the concentration of the parent compound in the solution. 2D Purity of isomeric compounds is reported as the sum of the isomers. Value is rounded to the nearest whole number after summation. 3 Based upon gravimetric preparation with balance calibration verified using NIST traceable weights (seven mass levels) and/or class A glassware used for dilutions. 4 Uncertainties determined using data for balances and glassware from measurement systems analysis methodology, raw material purity, and, when significant, equipment tolerances or calibration results. 5A Containers are overfilled to ensure the packaged amount, as a minimum. 5B Restek supplies deactivated vials along with most standards packed in 2 ml ampules, for the handling and storage of standards. Due to space constraints, Restek does not supply vials for larger volume ampules. Samples should be transferred into deactivated vials for handling and storage. Restek sells DMDCS for the purpose of glassware deactivation as catalog number 3184, which includes complete instructions. Restek will also deactivate larger volume vials from our inventory, as a custom ordered item. Contact your Restek sales or customer service representative for details. Manufactured under Restek's ISO 91:28 Registered Quality System Certificate #FM 8397 Note: Refer to data pack for complete raw material analysis. Page 3 of 11
Chemical Standard Batch Sheet Lot #: A82745 Catalog #: 3467 Target: 1. mg/ml Description: Delta-9-THC Standard Solvent: Methanol Solvent Lot: J47E7 Final Volume: ml Made by: Rebecca Lauver Date: 7/5/211 9:21:1AM Tested by: Diane Shaffer Pass Date: 7/5/211 2:55:45PM By: Chris Zucco Date: 7/6/211 8:38:32AM Packaged by: Joe Conway / Joe Conway Date: 7/5/211 11:35:38AM No. Units: 68 Pkg Size: Balance Used: ARMBALPC7 XP25 Serial #: 11283695 1 ml Compound delta-9-tetrahydrocannabinol 1972-8-3 CAS Storage Target Location Lot # Purity Conc(mg/mL) Target Actual Calc Conc(mg/mL) Vault MDG-197-25-2.93 1. 17.53 mg 17. mg 1. Page 4 of 11
QA Report: Delta-9-THA Standard (Cat.#3467) Runs of Lot # A82745 Runs of Lot # A81126 COMPONENT Run #1 Run #2 Run #3 AVG STD DEV % RSD Run #1 Run #2 Run #3 AVG STD DEV % RSD %D MEAN P/F Delta-9-Tetrahydrocannabinol 1623426 1643442 1613688 1626852 1517.93 17466 175867 17957 176515 22421 1.27-8. PASS Page 5 of 11 7/6/211 8:35:57 AM 1/1-1
Area % Report Page 1 of 1 Data File: \\Elmer\Ezchrom\Projects\standards\Data\delta-9-THC Lot#AMDG-197-25-2 Method: \\Elmer\Ezchrom\Projects\standards\Standardized Methods\RTX5-SV1.met Acquired: 3/29/211 8:22:1 PM Printed: 3/31/211 8:17:27 AM delta-9-thc Lot#AMDG-197-25-2 Volt 1..75..25 25.39 26.38 26.3 26.3 26.8 1..75..25 Volt 5 1 15 2 25 3 35 4 Minutes Front Results Retention Time Area Area Percent 25.39 76423 2.918 26.3 4994 1.95 26.3 2428146 92.75 26.38 3251 1.224 26.8 3277 1.249 Totals 2619. Page 6 of 11
** Search Report Page 1 of 1 ** Unknown: Scan 482 (25.714 min): DELTA-9-THC.D\data.ms Compound in Library Factor = 465 299 314 271 243 258 55 67 77 91 174 115 128 193 95 147 217 285 7 9 11 13 1 17 19 21 23 2 27 29 31 33 Hit 1 : Dronabinol C21H3O2; MF: 96; RMF: 966; Prob 91.9%; CAS: 1972-8-3; Lib: mainlib; ID: 151172. 299 314 OH 271 243 O 258 91 55 67 77 115 174 128 147 193 217 285 7 9 11 13 1 17 19 21 23 2 27 29 31 33 Page 7 of 11
** Search Report Page 1 of 1 ** Unknown: Scan 3942 (24.914 min): DELTA-9-THC.D\data.ms Compound in Library Factor = 429 55 67 79 91 17 121 174 134 147 193 246 161 27217 258 271281 299 314 7 9 11 13 1 17 19 21 23 2 27 29 31 Hit 1 : 1,3-Benzenediol, 2-[3-methyl-6-(1-methylethenyl)-2-cyclohexen-1-yl]-5-pentyl-, (1R-trans)- C21H3O2; MF: 924; RMF: 926; Prob 71.2%; CAS: 13956-29-1; Lib: mainlib; ID: 1387. OH HO 55 67 77 91 17 121 147 159 174 246 193 27217 258 271 286 299 314 7 9 11 13 1 17 19 21 23 2 27 29 31 Page 8 of 11
** Search Report Page 1 of 1 ** Unknown: Scan 445 (25.3 min): DELTA-9-THC.D\data.ms Compound in Library Factor = 336 55 67 77 91 174 17 119 134 147 165 187 193 21 215 7 9 11 13 1 17 19 21 23 2 27 29 31 33 246 258 271 281 299 314 Hit 1 :.DELTA.8-Tetrahydrocannabinol C21H3O2; MF: 9; RMF: 965; Prob 89.8%; CAS: 5957-75-5; Lib: replib; ID: 25244. OH 314 258 271 O 193 55 67 77 174 91 246 17 119 134 147 187 215 299 7 9 11 13 1 17 19 21 23 2 27 29 31 33 Page 9 of 11
** Search Report Page 1 of 1 ** Unknown: Scan 499 (25.811 min): DELTA-9-THC.D\data.ms Compound in Library Factor = -632 297 55 67 77 91 15 115 128 141 152 165 181 193 211 24 258 271 314 281 7 9 11 13 1 17 19 21 23 2 27 29 31 Hit 1 : Dronabinol C21H3O2; MF: 734; RMF: 765; Prob 51.9%; CAS: 1972-8-3; Lib: replib; ID: 26841. 299 OH 314 271 243 258 55 67 77 91 O 115 128 147 174 193 217 285 7 9 11 13 1 17 19 21 23 2 27 29 31 Page 1 of 11
** Search Report Page 1 of 1 ** Unknown: Scan 417 (26.217 min): DELTA-9-THC.D\data.ms Compound in Library Factor = 478 295 238 55 77 91 15 119 141 152 165 178 195 27 223 31 251 265 281 7 9 11 13 1 17 19 21 23 2 27 29 31 Hit 1 : Cannabinol C21H26O2; MF: 893; RMF: 918; Prob 97.3%; CAS: 521-35-7; Lib: replib; ID: 26764. 295 OH O 238 55 65 77 89 13 119 141 152 165 178 195 29 223 31 251 265 281 7 9 11 13 1 17 19 21 23 2 27 29 31 Page 11 of 11